microarray technique, analysis, and applications in dermatology jennifer villaseñor-park 1 and alex...
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![Page 1: Microarray Technique, Analysis, and Applications in Dermatology Jennifer Villaseñor-Park 1 and Alex G Ortega-Loayza 2 1 Department of Dermatology, University](https://reader036.vdocument.in/reader036/viewer/2022082700/55147114550346284e8b6052/html5/thumbnails/1.jpg)
Microarray Technique, Analysis, and Applications in Dermatology
Jennifer Villaseñor-Park1
and Alex G Ortega-Loayza2
1Department of Dermatology, University of Pittsburgh Medical Center; 2Virginia Commonwealth University
![Page 2: Microarray Technique, Analysis, and Applications in Dermatology Jennifer Villaseñor-Park 1 and Alex G Ortega-Loayza 2 1 Department of Dermatology, University](https://reader036.vdocument.in/reader036/viewer/2022082700/55147114550346284e8b6052/html5/thumbnails/2.jpg)
Microarray: Introduction
• Used for– Assessing gene expression levels– Genome-wide studies and genotyping– Evaluating microRNA levels
![Page 3: Microarray Technique, Analysis, and Applications in Dermatology Jennifer Villaseñor-Park 1 and Alex G Ortega-Loayza 2 1 Department of Dermatology, University](https://reader036.vdocument.in/reader036/viewer/2022082700/55147114550346284e8b6052/html5/thumbnails/3.jpg)
How do microarrays work?• Microarray chips are created that contain many
thousands of small DNA segments (probes) • Probes are arranged in a known and orderly
fashion (the “array”)• Sample mRNA or its corresponding cDNA is
labeled and exposed to the DNA probe on the chip
• If a given gene is expressed, the mRNA hybridizes to the DNA segment on the chip and can be detected
![Page 4: Microarray Technique, Analysis, and Applications in Dermatology Jennifer Villaseñor-Park 1 and Alex G Ortega-Loayza 2 1 Department of Dermatology, University](https://reader036.vdocument.in/reader036/viewer/2022082700/55147114550346284e8b6052/html5/thumbnails/4.jpg)
Microarrays
• Types of array platforms:– Filter arrays (i.e., “macroarrays”)
• Lower probe density compared to spotted or oligonucleotide arrays
• Lower sensitivity (e.g., low-abundance sequences may be missed)
– Spotted glass slide arrays– In situ synthesized oligonucleotide arrays
• Common probes– Complementary DNA (cDNA)– Oligonucleotides
![Page 5: Microarray Technique, Analysis, and Applications in Dermatology Jennifer Villaseñor-Park 1 and Alex G Ortega-Loayza 2 1 Department of Dermatology, University](https://reader036.vdocument.in/reader036/viewer/2022082700/55147114550346284e8b6052/html5/thumbnails/5.jpg)
Oligonucleotide arraysControl
cellsExperimental
cellsCy
3
Cy5
Targets
Hybr
idize
targ
ets t
o pr
obes
![Page 6: Microarray Technique, Analysis, and Applications in Dermatology Jennifer Villaseñor-Park 1 and Alex G Ortega-Loayza 2 1 Department of Dermatology, University](https://reader036.vdocument.in/reader036/viewer/2022082700/55147114550346284e8b6052/html5/thumbnails/6.jpg)
cDNA arraysControl
cellsExperimental
cellsCy
3
Cy5
Targets
Hybr
idize
targ
ets t
o pr
obes
![Page 7: Microarray Technique, Analysis, and Applications in Dermatology Jennifer Villaseñor-Park 1 and Alex G Ortega-Loayza 2 1 Department of Dermatology, University](https://reader036.vdocument.in/reader036/viewer/2022082700/55147114550346284e8b6052/html5/thumbnails/7.jpg)
Applications in dermatology
• Melanoma• Cutaneous T-cell lymphoma• Psoriasis• Scleroderma• Lupus erythematosus• Others