microbial metabolism enzymes sp10

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M I C R O B I A L M E T A B O L ISME N Z Y M E S

C H A PT E R 5



Metabolism: sum of all chemical reactionswithin an organism.

Metabolism:

1. Anabolism:

2. Catabolism:



Metabolic Reactions

A metabolic pathway is a sequence of

enzymatically catalyzed chemical reactions

in a cell which are determined by enzymes.

1. Exergonic

2. Endergonic



Enzymes:

Biological catalysts

Protein in nature

Reaction runs 8-10 billion times faster



Figure 5.2

Energy Requirements of a Chemical Reaction



Enzyme Structure

Holoenzyme

1.

Apoenzyme

2. Cofactor

1) Coe

nzymes

: N A D+,

FA D,

CoA2) Metal ions



Figure 5.3

Components of a Holoenzyme



Mechanism of Enzymatic Action

Active site of an enzyme

Enzyme-substrate complex

Specificity of active site



Figure 5.4a

The Mechanism of Enzymatic

Action



Relationship betweenEnzyme Structure and Function

10, 20

, 30 and 40structure of an enzyme

Change of enzyme shape

Change of active site----denaturation



Factors affecting enzymatic activity

Temperature

pH

Substrate concentration

Inhibitors



Figure 5.5a

Effect of Temperature on Enzyme Activity



Inhibitors

Competitive inhibition

Noncompetitive inhibition



Figure 5.7a b

Enzyme Inhibitors: Competitive Inhibition



Enzyme Inhibitors: Competitive Inhibition



Figure 5.7a, c

Enzyme Inhibitors: Noncompetitive Inhibition

microbial metabolism enzymes sp10

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