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Microbiological solutionsfor agri-food industries

Catalogue

PRINTED 05/2009

Contents

page

PATHOGENS 5VIDAS® 6VIDAS Reagents 8Ready-to-Use Media 9Food Safety Handbook -Microbiological Challenges 12

QUALITY INDICATORS 13TEMPO® 14TEMPO Reagents 16Ready-to-Use Media 17

IDENTIFICATION OF MICROORGANISMS 21VITEK® 2 Compact 22VITEK 2 Compact Reagents 24API®/ID 32/API® 20 25APIWEB™ 27Additional accessories 28

MICROBIAL GENOTYPING 30DiversiLab® 30

ENVIRONMENTAL CONTROL 31airIDEAL® 3P™ 32Count-Tact® Range 33Ready-To-Use Media 34

MICROBIAL DETECTION 35BacT/ALERT® 3D Microbial Detection System 36BacT/ALERT Reagents 38

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In Food Safety

• Pathogen detection: with culture media or automated with VIDAS®.

• Identification of microorganisms:with API® /ID 32 or automated with VITEK®

and DiversiLab® ranges.

In Food Quality

• Quality indicators enumeration:with culture media or automated with TEMPO®.

• Environmental control:with Count-Tact® rangeculture media, or with airIDEAL® 3P TM.

• Sterility and in-process testing:with culture media, or automated with BacT/ALERT®.

All these solutions haveperformances adapted to yourdecision criteria, for raw materialqualification, in-process control (HACCP, environmental…) or finished product testing to defineshelf life.

To take into account specificcountry cultures, manufacturingprocesses, eating habits…,bioMérieux validates its solutionsfor a large variety of food products.Its worldwide presence plays anessential role in understandingwhat solution to provide. A major part of analyses are driven by laboratory needs foraccreditation according tointernational norms like ISO.With this in mind, bioMérieux submits its analytical solutions to international and national organisms for official validationsand approval such as AOACInternational, AFNOR, etc.

bioMérieux is always keen toreceive questions or suggestions about our products in order to better understand andserve specific industries. For any additional information,please, contact local bioMérieux representative.

bioMérieux Industry is strongly

involved in the protection of health

and life quality of consumers,

it therefore offers a wide range of

solutions for microbiological control,

from sample preparation to final

identification of microorganisms,

in the field of agri-food industries,

such as meat, poultry, dairy products,

chilled food… etc.

Pathogens

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AUTOMATED METHOD 6

VIDAS® 6VIDAS Reagents 8

READY-TO-USE MEDIA 9

BIOMÉRIEUX EDITIONS 12

Food Safety Handbook: 12Microbiological Challenges

Pathogens

AUTOMATED METHOD

VIDASVIDAS®

VIDAS TECHNOLOGY

6

N°1 Automated Solution for Rapid Pathogen Monitoring

A fixed antibody captures thetarget pathogen and a secondantibody conjugated withan enzyme fixes itself to thecaptured antigen.

The intensity of the fluorescenceis interpreted by the system.

Buffers and substrate are readyto use in the strip. All pipettingsteps are made automaticallywith the SPR®.

The computer module, withWINDOWS NT is used to manageand print out the results. It can handle up to two analyticalmodules simultaneously, givingthe system a capacity of 60 testsper hour.miniVIDAS® is a compact versionof the VIDAS system, with a built-in computer, keyboard and printer.

It has 2 independent compart-ments each accepting 6 tests, and can process up to 12 samplessimultaneously.VIDAS and miniVIDAS havebeen already chosen by thousands of food laboratories worldwide.

VIDAS is a multi-parameterautomated immunoanalyser.It includes an analytical module, a computer and a printer.The analytical module automati-cally performs all stages of theanalysis, including printing outthe full results. It contains fiveindependant compartments, each accepting up to 6 tests.

Productivity

• Throughput adapted to highvolume or single test.

• Optimized protocols for a flexibledaily workflow.

• Results within one hour afterenrichment.

• Rapid screening of samples.• Connectivity with laboratory

IT network.

Ease of use

• The analysis is entirely automatedfrom inserting the sample to reporting results.

• With ready-to-use kits, manipula-tions are reduced to a minimum.

• VIDAS® provides a completesolution: reagents and ready to use media.

State ot the art technology for all food laboratories

• A range in constant innovation to meet your evolving needs: 2nd generation tests, Next daysolutions, modified antibodies to increase specificicty.

• Innovative reagents and mediato improve performances.

VIDAS NEXT DAY

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VIDAS® yields a Next Daysolution.A complete result for presenceor absence allows rapid corrective action or a quickrelease of final product.VIDAS Next Day is available for Salmonella, with ICS + SLMand ICS2 + SLM*, for Listeriawith LSX, for E. coli O157with ECPT.VIDAS Next Day is validatedby both AFNOR and AOAC.By reducing the time to a NextDay result, VIDAS brings yousignificant operational profit:increased flexibility andefficiency, improved productivityand space optimization.

*Consult us for availability.

Optimal reliability

• The benefits of automationincludes an objective interpretation of results.

• Reduced risk due to manipulationor cross contamination duringthe analysis.

• Each analysis has completetraceability (bar code, completetest report).

• A wide range of official validationfor the reagents.

VIDAS Heat & GoA dry heating system, designed to heat 12 strips simultaneously.It enhances traceability and productivityand reduces risks associated with heating step.

VIDAS UP E. coli 0157 (including H7)is the latest innovation on VIDAS® using the recombinant phage protein technology.

Pathogens

AUTOMATED METHODS

8

VIDAS® ReagentsVIDAS® ReagentsIn order to assure reliabilityfor pathogen detection withVIDAS, bioMérieux has developeda range of enrichment mediawhere performance is controlledusing VIDAS as well as a largechoice of selective media.

For optimal use, these media areprovided in ready-to-use format(see page 9).bioMérieux also offers chromogenic media and the APIrange, the worldwide referencefor identification, for a practicaland standardized confirmation of positive results.

Solution Time to result ISO 16140/AFNOR AOAC HPBC EMMAS

SSLLMM 22 DDaayyss ✔ ✔ ✔ ✔

IICCSS ++ ppllaattee 22 DDaayyss ✔ ✔

IICCSS22 ++ ppllaattee** 22 DDaayyss ✔

LLIISS 22 DDaayyss ✔ ✔ ✔ ✔

LLMMOO22 22 DDaayyss ✔ ✔ ✔

LLDDUUOO 22 DDaayyss ✔ ✔

CCAAMM 22 DDaayyss SSEETT22 << 2244 hhoouurrss ✔

VIDAS NEXT DAY

ICS + SLM 1 Day ✔ ✔

ICS2 + SLM* 1 Day ✔

LSX 1 Day ✔ ✔

ECO up to 1 Day ✔ ✔

ECPT up to 1 Day ✔** ✔**

*Consult us for availability

**On going validations

VIDAS: A WORLDWIDE REFERENCE

SalmonellaVIDAS Salmonella SLM Detection of Salmonella in food products and 60 tests 30702

environmental samplesVIDAS Immuno-Concentration Salmonella ICS Immuno-concentration of Salmonella in food products 60 tests 30435VIDAS Immuno-Concentration Salmonella II* ICS2 Immuno-concentration of Salmonella in food products 60 tests 30708*

ListeriaVIDAS Listeria LIS Detection of Listeria in food products and 60 tests 30700

environmental samplesVIDAS Listeria Species Xpress LSX Detection of Listeria in selected food products 60 tests 30224

and environmental samplesVIDAS Listeria monocytogenes II LMO2 Detection of Listeria monocytogenes in food products 60 tests 30704

and environmental samplesVIDAS Listeria Duo LDUO Simultaneous and differenciated detection of Listeria

monocytogenes in food products and environmental samples 60 tests 30225

E. coli O157VIDAS E. coli O157 ECO Detection of E. coli O157 in food products 30 tests 30112VIDAS Immuno-Concentration E. coli O157 ICE Facilitates confirmation of E. coli O157 on 30 tests 30526

specific culture media

VIDAS UP E. coli O157 (including H7) ECPT Facilitates confirmation of E. coli O157 on 30 tests 30122specific culture media including H7

CampylobacterVIDAS Campylobacter CAM Detection of Campylobacter in food products 30 tests 30111

Staphylococcal enterotoxinsVIDAS Staph enterotoxin II SET2 Detection of staphylococcal enterotoxins A, B, 30 tests 30705

C1, C2, C3, D and E in food products


Pathogens

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Ready-to-Use MediaReady-to-Use MediaFood culture media solutions

42606 Cefixime - Tellurite Mixture (NF EN ISO 16654) ●Selective isolation of Escherichia coli O157: H7 6 x 4 ml (lyophilized)

42605 Chrom ID 0157:H7 (O157H7 ID-F) (NF EN ISO 16654) ●Chromogenic medium for the selective isolation and 6 x 200 mlpresumptive identification of Escherichia coli O157: H7

42630 ChromID 0157:H7 (O157H7 ID-F) (NF EN ISO 16654) ●Chromogenic medium for the selective isolation and presumptive 5 x 20 ml identification of Escherichia coli O157: H7

51015 Mac Conkey broth ●Selective enrichment for the VIDAS ECO 500 g

43391 SMAC CT Agar (NF EN ISO 16654) ●Selective isolation of Escherichia coli O157: H7 2 x 10 (90 mm)

42042 Buffered Peptone Water (NF EN ISO 6579 • NF EN ISO 6887-1 • NF EN ISO 11290-2) ●Diluent and pre-enrichment medium for the detection 6 x 90 mlof Salmonella in food products


42111 Buffered Peptone Water (NF EN ISO 6579 • NF EN ISO 6887-1 • NF EN ISO 11290-2) ●Diluent and pre-enrichment medium for the detection of Salmonella 100 x 9 mlin food products

42629 Buffered Peptone Water (NF EN ISO 6579 • NF EN ISO 6887-1 • NF EN ISO 11290-2) Other packagingDiluent and pre-enrichment medium for the detection of Salmonella 3 x 3 liter bagsin food products (Bags)

42729 Buffered Peptone Water (NF EN ISO 6579 • NF EN ISO 6887-1 • NF EN ISO 11290-2) Other packagingDiluent and pre-enrichment medium for the detection of Salmonella 10 x 225 ml Minibagsin food products (Minibags)

51094 Buffered Peptone Water (NF EN ISO 6579 • NF EN ISO 6887-1 • NF EN ISO 11290-2) ●Diluent and pre-enrichment medium for the detection of 500 gSalmonella in food products

43641 ChromID Ottaviani Agosti (OAA) (NF EN ISO 11290) ●Chromogenic medium for the selective isolation, enumeration 2 x 10 (90 mm)and presumptive identification of Listeria monocytogenes

43649 ChromID Ottaviani Agosti* (OAA) (NF EN ISO 11290) ●Chromogenic medium for the selective isolation, enumeration 10 x 10 (90 mm)and presumptive identification of Listeria monocytogenes

42046 FRASER Broth ●Selective pre-enrichment of Listeria in food products 6 x 225 ml

42072 FRASER Broth (NF EN ISO 11290-1) ●Selective enrichment of Listeria in food products 20 x 10 ml

42048 Half-FRASER Broth (NF EN ISO 11290-1) ●Selective pre-enrichment of Listeria in food or environmental products 6 x 225 ml

43741 ChromID Sakazakii (ESPM) ●Chromogenic medium for the selective isolation of E.sakazakii in infant food products. 2 x 10 (90 mm)

Tube Bottle Plate Dehydratedmedia

A wide range of culture media, specifically devised and evaluated forfood testing, is available to detectpathogens such as Listeria monocyto-genes, Salmonella, E. coli O157:H7,Campylobacter, Vibrio*, Enterobactersakazakii (Cronobacter).bioMérieux is ISO 9001-certified andcontrols all the stages of production, frommedium design (selection to each rawmaterial) through to manufacture, withperfect traceability.By means of rigorous raw material selec-tion, significant control of production andshelf-life validation according to a proce-dure including thermal shocks, bioMérieuxcan guarantee the performance androbustness of its ready-to-use media.The standardized formulas comply withfood industry standard recommendations

(generally NF, EN and/or ISO). They areadjusted and supplemented, as authorizedby the standard ISO/TS 11133-2:2003,to achieve the expected performancelevels and allow satisfactory equivalencebetween lots. Similarly, the quality controlprocedures performed on these mediacomply with the recommendations of the standard ISO/TS 11133-2 and thiscompliance has been evaluated byAFAQ/AFNOR Certification.Chromogenic culture mediaOn the basis of API® technology used todetect enzyme reactions, bioMérieux hasdevised a wide range of chromogenicmedia, named “chromID™”, including a specific range for food testing.These media, some of which arestandardized, offer very easy useand better performance criteria than conventional media.

They can be used according to short andsimple protocols, and validated by AFNORCertification.The range of chromogenic media intendedto screen for pathogens currently consistsof five* media:chromID Salmonella, chromID OttavianiAgosti, chromID O157:H7, chromIDSakazakii, chromID Vibrio*

E . c o l i O 1 5 7

E n t e r o b a c t e r s a k a z a k i i

L i s t e r i a

Pathogens

Ready-to-Use MediaReady-to-Use Media

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42112 Half-FRASER Broth (NF EN ISO 11290-1) ●Selective pre-enrichment of Listeria in environmental samples 20 x 10 ml

42615 Half-FRASER Broth (NF EN ISO 11290-1) ●Selective pre-enrichment of Listeria in food or environmental products 4 x 225 ml(for direct inoculation of raw milk)

42627 Half-FRASER Broth (NF EN ISO 11290-1) Other packagingSelective pre-enrichment of Listeria in food products (Bags) 3 x 3 liter bags

42727 Half-FRASER Broth (NF EN ISO 11290-1) Other packagingSelective pre-enrichment of Listeria in food products (Minibags) 10 x 225 Minibags

42117 LX Broth ●Broth for the selective enrichment of Listeria in food products. 20 x 6 mlFor use with the VIDAS® system

42120 LX Broth ●Broth for the selective enrichment of Listeria in food products. 20 x 10 mlFor use with the VIDAS® system

42625 LX Broth ●Broth for the selective enrichment of Listeria in raw milk samples. 4 x 225 mlFor use with the VIDAS system

42626 LX Broth ●Broth for the selective enrichment of Listeria in food products. 6 x 225 mlFor use with the VIDAS system

42628 LX Broth Other packagingBroth for the selective enrichment of Listeria in food products. 3 x 3 liter bagsFor use with the VIDAS system (Bags)

42635 LX Broth ●Broth for the selective enrichment of Listeria in food products. 6 x 100 mlFor use with the VIDAS system

43560 Oxford Agar (NF EN ISO 11290) ●Selective medium for isolation and enumeration of Listeria in food products 2 x 10 (90 mm)

43559 PALCAM Agar (NF EN ISO 11290) ●Selective medium for isolation and enumeration of Listeria in food products 2 x 10 (90 mm)



42111 Buffered Peptone Water (NF EN ISO 6579 • NF EN ISO 6887-1 • NF EN ISO 11290-2) ●Diluent and pre-enrichment medium for the detection of Salmonella 100 x 9 mlin food products

42629 Buffered Peptone Water (NF EN ISO 6579 • NF EN ISO 6887-1 • NF EN ISO 11290-2) Other packagingDiluent and pre-enrichment medium for the detection of Salmonella 3 x 3 liter bagsin food products (Bags)

42729 Buffered Peptone Water (NF EN ISO 6579 • NF EN ISO 6887-1 • NF EN ISO 11290-2) Other packagingDiluent and pre-enrichment medium for the detectionof Salmonella 10 x 225 ml Minibagsin food products (Minibags)

51094 Buffered Peptone Water (NF EN ISO 6579 • NF EN ISO 6887-1 • NF EN ISO 11290-2) ●Diluent and pre-enrichment medium for the detection of 500 gSalmonella in food products

43621 ChromID Salmonella (SM2) (NF EN ISO 6579) ●Chromogenic medium for the selective isolation 2 x 10 (90 mm)and differentiation of the genus Salmonella

43629 ChromID Salmonella (SM2) (NF EN ISO 6579) ●Chromogenic medium for the selective isolation and differentiation 10 x 10 (90 mm)of the genus Salmonella

43111 Hektoen Agar (NF EN ISO 6579) ●Selective isolation of Salmonella and Shigella 2 x 10 (90 mm)

43119 Hektoen Agar (NF EN ISO 6579) ●Selective isolation of Salmonella and Shigella 10 x 10 (90 mm)

51050 Hektoen (NF EN ISO 6579) ●Selective isolation of Salmonella and Shigella 500 g

42610 ICS Broth ●Non selective broth for the development of Salmonella 100 x 2 mlfollowing immuno-enrichment using the VIDAS® ICS

42077 M Broth ●Selective enrichment broth for the detection of Salmonella in food products 20 x 10 mlusing the VIDAS SLM.

43588 Modified Brillant Green Agar (NF EN ISO 6579) ●Selective isolation of Salmonella in food products 2 x 10 (90 mm)

42639 MSRV ●Modified semisolid Rappaport Vassiliadis for enrichment of Salmonella 6 x 225 ml

Tube Bottle Plate DehydratedmediaL i s t e r i a

S a l m o n e l l a

Pathogens


42114 Muller-Kauffmannn Broth (NF EN ISO 6579) MKTTn ●Selective enrichment broth for the detection of Salmonella in food products 20 x 10 ml

42073 Rappaport Vassiliadis Broth ●Selective enrichment broth for detection of Salmonella in food products 20 x 10 ml

42110 Rappaport Vassiliadis Soy Broth (NF EN ISO 6579) ●Selective enrichment broth for the detection of Salmonella in food products 20 x 10 ml

42052 Selenite Cystine Broth ●Selective enrichment broth for the detection of Salmonella in food products 20 x 10 ml

43291 SM® ID Agar ●Chromogenic medium for the selective isolation and differentiation of Salmonella 2 x 10 (90 mm)

43299 SM® ID Agar ●Chromogenic medium for the selective isolation and differentiation of Salmonella 10 x 10 (90 mm)

42121 SX2 Broth ●Selective enrichment for the Salmonella detection using the VIDAS SLM 20 x 10 ml(Food and environmental samples)

43563 XLD Agar (NF EN ISO 6579) ●Selective isolation of Salmonella and Shigella 2 x 10 (90 mm)

43564 XLD Agar (NF EN ISO 6579) ●Selective isolation of Salmonella and Shigella 10 x 10 (90 mm)

51049 XLD Agar (NF EN ISO 6579) ●Selective isolation of Salmonella and Shigella 500 g

43701 XLT4 Agar (ISO 6579 Annex D) ●Selective isolation of Salmonella 2x10 (90mm)

43111 Hektoen Agar ●Selective isolation of Salmonella and Shigella 2 x 10 (90 mm)

43119 Hektoen Agar ●Selective isolation of Salmonella and Shigella 10 x 10 (90 mm)

51050 Hektoen Enteric Agar ●Selective isolation of Salmonella and Shigella 500 g

43761 ChromID™ Vibrio (VID)* ●Chromogenic medium for the selective isolation of Vibrio and the presomptive identification of V. parahaemolyticus and V. cholerae

2 x 10 (90 mm)

43209 Yersinia CIN Agar (NF EN ISO 10273) ●Selective isolation of Yersinia 10 x 10 (90 mm)

43421 Yersinia CIN Agar (NF EN ISO 10273) ●Selective isolation of Yersinia 2 x 10 (90 mm)

42606 Cefixime - Tellurite Mixture (NF EN ISO 16654) ●Selective isolation of Escherichia coli O157: H7 6 x4ml (lyophilized)

43471 Campy Food Agar ●Selective isolation and enumeration of Campylobacter in food 2 x 10 (90 mm)and environmental samples

42642 Campy Food Broth* Other packagingSelective enrichment of Campylobacter in food and environmental samples 10 x 90 ml (Minibags)

42643 Campy Food Broth* Other packagingSelective enrichment of Campylobacter in food and environmental samples 10 x 225 ml (Minibags)

30551 Combibags Other packagingStomacher bags for Campylobacter enrichment to be used with Genbox microaer 3 x 10 (Bags)

96125 Genbox Microaer Other packagingAtmosphere generators Kit comprise: 10 aluminium sachets

each containing 1 generator

Tube Bottle Plate DehydratedmediaS a l m o n e l l a

99629 Pinch Dilutor*Gravimetric system for the automatic sample dilution using 3 liter bags. Can be used for preparing samples for pathogens detection as well as quality indicators enumeration.

99638 User manual CD

99641 Silicone tubing

99640 Stainless steel needle


A d d i t i o n a l e q u i p m e n t

Ye r s i n i a

S u p p l e m e n t

C a m p y l o b a c t e r

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PathogensbioMérieux Editions

12

FOOD SAFETY HANDBOOK: Microbiological Challenges

The Food Safety Handbook presents an easy to read overviewon the current worldwide foodsafety situation and explains thechallenges facing the array of stake-holders along the food chain in thecontext of a global food market.It provides extensive information on today's important foodbornepathogens and includes other related food safety topics, from the

implementation of HACCP plans, to future laboratory diagnostic toolsand emerging foodborne pathogensetc.The book benefits from the experience of more than 20 international experts with diverseexpertise and styles. It aims to provide a modern approach to thisincreasingly complex issue.

00118 FOOD SAFETY HANDBOOK: Microbiological Challenges

Language: EnglishFeatures: 240 pages, 21.5 x 27.5 cm / hardback cover

Date of Publication: December 2007

Publisher: bioMérieux

ISBN: 978-2-917162-08-8

Authors: Jean-Yves D'AOUST, Reisha BARNES, Mark CARTER,Stephenie Lynn DRAKE, Dave EVANSON, Séamus FANNING,Russel FLOWERS, Valerio GIACCONE, Lee-Ann JAYKUS, Jean-Louis JOUVE, Jean KENNEDY, Jan McCLURE, Ann Marie McNAMARA, Mogens MADSEN, Truls NESBAKKEN,Servé NOTERMANS, Stephen O'BRIEN, Franco OTTAVIANI, Milena OTTAVIANI, Michele SMOOT, Bala SWAMINATHAN,Christine VERNOZY-ROZAND, Patrick WALL, Paul WHYTE

F o o d S a f e t y

Quality indicators

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AUTOMATED METHOD 14

TEMPO® 14TEMPO Reagents 16

READY-TO-USE MEDIA 17

The agri-food industry routinely enumerates microbial flora, which indicatesthe microbiological quality of the entire production process, from raw materialsto finished products. Quality indicators are of major importance in establishinga HACCP approach and guarantee the commercial value of food products fromleaving the factory through to the end of their shelf life.Quality indicators enumerated in routine usually are:Total count, total/thermo tolerant coliforms, E. coli, S. aureus,Enterobacteriaceae, yeast & moulds…

Quality indicators

AUTOMATED METHODS

TEMPOTEMPO®

TEMPO® is an automated solution for the enumeration of quality indicators in food. One TEMPO configuration performs up to 500 tests per day. The TEMPO solution iscomposed of two ergonomic works stations:

• The preparation station to inoculate TEMPO cardswith the food sample and specific culture media.

• The reading station to read TEMPO cards and givefinal result in CFU/g of initial product.

It comprises of: • computer with a waterproof mini-keyboard, barcode

scanner and a WIFI card;• intuitive preparation software;• 144 tests capacity media stand;• 4 dispensers for media rehydratation;• TEMPO Filler instrument for inoculating and sealing

the cards;• 4 filling trays;• 12 incubation/reading trays.

It comprises of: • computer with a barcode scanner and a mouse;• intuitive software for reading and managing results;• administration software for managing the

configuration;• TEMPO Reader instrument for reading the cards;• wireless WIFI transmission router;• printer;• bidirectional connection with the lab computer

(optional).

PRINCIPLE

14

Relayed through a wireless WIFI connection, the two stations communicate in real time although they are not in the same room.

Each medium containsa fluorescent indicator basedon the formula of traditional

culture media and bioMérieux’sexpertise in bacteriology.

The TEMPO cardoffers the precision

of the MPN method in 16 tubes.

Ease of use

Using intuitive icons and userfriendly software, TEMPO® is veryeasy to operate and requiresless than two days of training to beroutinely used.

Standardisation

As most of the analytical stepsare automated, TEMPO improvesthe consistency of results fromplant to plant, day to day, countryto country and therefore operator variability is removed.

Time savings

With TEMPO results are one totwo days faster than conventionalmethods (i.e: 24 to 48 h), providing more rapid selection and orientation of raw materials,accelerated corrective actions in case of non compliance, speedier product release, improvedstock rotation…As the routine workflow is fullyoptimized, time is saved for otheractivities such as statistical andtrend analysis, monitoring ofcritical control points, reinforcing ofpathogen control plans or simplyperforming more routine analyses.

Cost savings

The TEMPO card covers severaldilutions, so only one TEMPO testis needed instead of multipleplates to obtain the most relevantresult according to each criteria. It also eliminates time consumingtasks such as media preparation,manual dilutions and identifications.

Traceability

Every consumable item is tracedthrough a barcode identification.The unique card barcode ensuresfull traceability throughout

the analytical procedure, from the sample ID until the final result.All data is saved and can be easilyretrieved for quality assurance purposes.

Innovation

bioMérieux using its experience and know-how in culture media, instrumentation, VITEK® cards and fluorescent reading, has developed TEMPO, the first automated solution for quality indicator enumeration.

15

After distribution of the culture media-sample mixture inthe wells, the TEMPO cards are placed into a normal incubator.The bacteria multiply during incubation and metabolisethe culture media.

According to the number and size ofthe positive wells (fluorescent or non-fluorescent), the TEMPO systemuses statistical methods to calculate the number of microorganisms present in the initial sample.

The finalresult isexpressed inCFU/g

The card, the heart of TEMPO system.

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TEMPO® ReagentsTEMPO® Reagents

Quality indicators

AUTOMATED METHODS

The TEMPO test is composed ofone vial of specific media and one associated card.

Product ISO 16140/AFNOR AOAC

TTEEMMPPOO TTVVCC ✔ ✔

TTEEMMPPOO TTCC ✔

TTEEMMPPOO CCCC ✔

TTEEMMPPOO EECC ✔ ✔

TTEEMMPPOO EEBB ✔ ✔

* At the date of printing

INTERNATIONAL VALIDATIONS FOR OFFICIAL RECOGNITION*

Product description Product number Quantity per package

TEMPO TVC 80007 48 testsEnumeration of aerobic mesophilic total flora in 40-48 hours

TEMPO EB 80003 48 testsEnumeration of Enterobacteriaceae in 24 hours

TEMPO TC 80006 48 testsEnumeration of total coliforms according to the ISO 4832 definition in 24 hours

TEMPO CC 80044 48 testsEnumeration of coliforms according to the FDA - BAM definition in 24 hours

TEMPO EC 80004 48 testsEnumeration of Escherichia coli in 24 hours

TEMPO STA 80002 48 testsEnumeration of Staph in 24 hours

TEMPO YM 80001 48 testsEnumeration of yeast and molds in 72 hours

TEMPO LAB 80071 48 testsEnumeration of lactic acid bacteria in 40-48 hours

TEMPO QC 80000 One testFor a periodical control of the TEMPO system

TEMPO Dispenser bottle 80014 10 bottlesGlass bottle of 1 liter capacity

TEMPO bags 80015 20x25 bagsFiltering bags for sample preparation

TEMPO Reader tray 80012 1 trayAdditional incubation/reading trays (20 positions)

Quality indicators

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Ready-to-Use MediaReady-to-Use MediaFood culture media solutions

42603 Iron Sulfite Agar (NF ISO 15213 • NF EN ISO 7937) ●Enumeration of sulfite-reducing bacteria growing under anaerobic conditions 6 x 200 mland Clostridium perfringens in food products

43401 Schaedler Agar + 5% Sheep Blood ●Isolation of anaerobic bacteria 1 x 20 (90 mm)

43279 Schaedler Agar + 5% Sheep Blood ●Isolation of anaerobic bacteria 10 x 10 (90 mm)

43301 Mueller Hinton 2 Agar ●Susceptibility to antibiotics and sulfonamides 2 x 10 (90 mm)



43321 Mueller Hinton 2 Agar + 5% Sheep Blood ●Susceptibility of pneumococci and other streptococci to antibiotics and sulfonamides 2 x 10 (90 mm)

43329 Mueller Hinton 2 Agar + 5% Sheep Blood ●Susceptibility of pneumococci and other streptococci to antibiotics and sulfonamides 10 x 10 (90 mm)

41864 Mueller Hinton 2 Agar ●Susceptibility to antibiotics and sulfonamides 6 x 200 ml

42619 D-cycloserine Supplement (NF ISO 15213 • NF EN ISO 7937 • NF V 08-056 • NF XP V 08-061) Other packagingEnumeration of Clostridium perfringens in food products 6 x 2 ml

42603 Iron Sulfite Agar (NF ISO 15213 • NF EN ISO 7937 • NF V 08-056 • NF XP V 08-061) ●Enumeration of sulfite-reducing bacteria growing under anaerobic conditions 6 x 200 mland Clostridium perfringens in food products

51048 TSN Agar ●Trypcase, Sulfite, Neomycin - Isolation of Clostridium perfringens 500 g

42017 chromID coli (coli ID) ●Selective and chromogenic medium for the detection and enumeration 6 x 200 mlof ß D-glucuronidase positive E. coli and other coliforms from food samples

51027 Desoxycholate Lactose Agar ●Isolation and enumeration of coliform bacteria in food products 500 g

51032 Endo Agar ●Selective isolation of enterobacteria and coliform organisms 500 g

42617 VRBL Agar (NF ISO 4832 • NF 08-050 • NF V08-060) ●Enumeration of coliforms and thermotolerant coliforms in food products 6 x 200 ml

Tube Bottle Plate DehydratedmediaA n a e r o b i c b a c t e r i a

A n t i b i o g r a m

C l o s t r i d i u m p e r f r i n g e n s

C o l i f o r m s / E . c o l i

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A wide range of culture media, specifically designed and evaluatedfor food testing, is available toenumerate quality indicator microorganisms such as Escherichiacoli, Enterobacteriaceae,Staphylococcus, yeasts/molds, sulfite-reducing anaerobes, etc..bioMérieux is ISO 9001-certified andcontrols all the stages of production, frommedium design (selection to each rawmaterial) through to manufacture, withperfect traceability.By means of rigorous raw material selec-tion, significant control of production andshelf-life validation according to a proce-dure including thermal shocks, bioMérieuxcan guarantee the performance androbustness of its ready-to-use media.

The standardized formulas comply withfood industry standard recommendations(generally NF, EN and/or ISO). They areadjusted and supplemented, as authorizedby the standard ISO/TS 11133-2:2003,to achieve the expected performancelevels and allow satisfactory equivalencebetween lots. Similarly, the quality controlprocedures performed on these mediacomply with the recommendations of the standard ISO/TS 11133-2 and thiscompliance has been evaluated byAFAQ/AFNOR Certification (2006).Chromogenic culture mediaOn the basis of API® technology used todetect enzyme reactions, bioMérieux hasdevised a wide range of chromogenicmedia, named “chromID™”, including aspecific range for food testing.

These media, some of which are standar-dized, offer very easy use and better per-formance criteria than conventional media. The range of chromogenic media intendedto screen for pathogens currently consistsof five media (see page 9).ChromID Coli medium is specifically designed for the simultaneous detectionand enumeration of E. coli and coliforms. It can be used at 2 incubation temperatures(37°C or 44°C) according to a protocolvalidated by AFNOR Certification.

Quality indicators

18


42111 Buffered Peptone Water (NF EN ISO 6579 • NF EN ISO 6887-1 • NF EN ISO 11290-2) ●Diluent and pre-enrichment medium for the detection of Salmonella in food products 100 x 9 ml (BPW-T)

51094 Buffered Peptone Water (NF EN ISO 6579 • NF EN ISO 6887-1 • NF EN ISO 11290-2) ●Diluent and pre-enrichment medium for the detection of Salmonella in food products 500 g

42042 Buffered Peptone Water (NF EN ISO 6579 • NF EN ISO 6887-1 • NF EN ISO 11290-2) ●Diluent and pre-enrichment medium for the detection of Salmonella in food products 6 x 90 ml

42043 Buffered Peptone Water (NF EN ISO 6579 • NF EN ISO 6887-1 • NF EN ISO 11290-2) ●Diluent and pre-enrichment medium for the detection of Salmonella in food products 6 x 225 ml (BPW-F)

42629 Buffered Peptone Water (NF EN ISO 6579 • NF EN ISO 6887-1 • NF EN ISO 11290-2) (Bags) Other packagingDiluent and pre-enrichment medium for the detection of Salmonella in food products 3 x 3 liter bags

42729 Buffered Peptone Water (NF EN ISO 6579 • NF EN ISO 6887-1 • NF EN ISO 11290-2) (Minibags) Other packagingDiluent and pre-enrichment medium for the detection of Salmonella in food products 10 x 225 ml Minibags

42021 Peptone Water (MRD) ●Diluent for the preparation of stock solutions and dilutions 6 x 90 ml (MRD-F)

42076 Peptone Water (MRD) ●Diluent for the preparation of stock solutions and dilutions 20 x 9 ml (MRD-T)

99629 Pinch Dilutor*Gravimetric system for the automatic sample dilution using 3 liter bags See Pathogens part for more informations

42113 Saline Solution ●Universal diluent 100 x 9 ml

43081 Eosin-Methylene Blue Agar ●Isolation of enterobacteria 2 x 10 (90 mm)

42087 Kliger Agar ●Differentiation of enterobacteria 20 (slope + base)

42601 VRBG Agar (NF ISO 21528-1 and 21528-2) ●Selective medium for isolation and enumeration of enterobacteria 6 x 200 ml

43151 D-Coccosel Agar ●Selective isolation of enterococci and group D streptococci 2 x 10 (90 mm)

51025 D-Coccosel Agar ●Selective isolation and differentiation of enterococci and group D streptococci 500 g

43041 Columbia Agar + 5% Sheep Blood ●Isolation of fastidious bacteria. Detection of hemolysis 2 x 10 (90 mm)

43049 Columbia Agar + 5% Sheep Blood ●Isolation of fastidious bacteria. Detection of hemolysis 10 x 10 (90 mm)

43050 Columbia Agar + 5% Horse Blood ●Isolation of fastidious bacteria. Detection of hemolysis 2 x 10 (90 mm)

43059 Columbia Agar + 5% Horse Blood ●Isolation of fastidious bacteria. Detection of hemolysis 10 x 10 (90 mm)

43071 Columbia ANC Agar + 5% Sheep Blood ●Selective isolation of fastidious bacteria. Detection of hemolysis 2 x 10 (90 mm)

43079 Columbia ANC Agar + 5% Sheep Blood ●Selective isolation of fastidious bacteria. Detection of hemolysis 10 x 10 (90 mm)

43001 Trypcase Soy Agar + 5% Sheep Blood ●Isolation of bacteria. Determination of hemolysis 2 x 10 (90 mm)

43009 Trypcase Soy Agar + 5% Sheep Blood ●Isolation of bacteria. Determination of hemolysis 10 x 10 (90 mm)

43061 Trypcase Soy Agar + 5% Horse Blood ●Isolation of bacteria. Determination of hemolysis 2 x 10 (90 mm)

96118 Anaer Indicator Other packagingAnaerobic atmosphere indicator 50 strips

30551 Combibag Other packagingStomacher bags for Campylobacter enrichment to be used with Genbox microaer 3 x 10 bags

45532 GENbag Microaer Other packagingAtmosphere generators Kits comprise: 20 airlight bags - 20 generators - 2 dip seals

45533 GENbag CO2 Other packagingAtmosphere generators Kits comprise: 20 airlight bags - 20 generators - 2 dip seals

45534 GENbag Anaer Other packagingAtmosphere generators Kits comprise: 20 airlight bags - 20 generators - 2 dip seals

96124 GENbox Anaer Other packagingAtmosphere generators anaer: 10 aluminium sachets each containing 1 generator

96125 GENbox Microaer Other packagingAtmosphere generators microaer: 10 aluminium sachets each containing 1 generator

96126 GENbox CO2 Other packagingAtmosphere generators CO2: 10 aluminium sachets each containing 1 generator

96127 GENbox Jar 2,5 l Other packagingIncubation jars GENbox jar 2,5 L

96128 GENbox Jar 7,0 l Other packagingIncubation jars GENbox jar 7,0 L


E n t e r o c o c c u s

H e m o l y t i c b a c t e r i a

I n c u b a t i o n s y s t e m s

E n t e r o b a c t e r i a c e a e

D i l u e n t



42602 MRS Agar (NF ISO 15214) ●Enumeration of mesophilic lactic acid bacteria 6 x 200 ml

43565 Cetrimide Agar ●Selective isolation of Pseudomonas aeruginosa 2 x 10 (90 mm)

43521 Baird Parker Agar (NF EN ISO 6888-1) ●Enumeration of coagulase positive Staphylococcus in food products 2 x 10 (90 mm)

43529 Baird Parker Agar (NF EN ISO 6888-1) ●Enumeration of coagulase positive Staphylococcus in food products 10 x 10 (90 mm)

43531 Baird Parker RPF Agar (NF EN ISO 6888-2) ●Enumeration without confirmation of coagulase positive staphylococci/Coagulase test 2 x 10 (90 mm)

43539 Baird Parker RPF Agar (NF EN ISO 6888-2) ●Enumeration without confirmation of coagulase positive staphylococci/Coagulase test 10 x 10 (90 mm)

44003 Baird Parker RPF Agar (NF EN ISO 6888-2) ●Enumeration without confirmation of coagulase-positive staphylococci 4 x 90 ± 2 ml of Baird Parker base: R1 + 4 x 10 ml of RPF (Lyoph.): R2

55181 Lyophilized Rabbit Plasma (NF EN ISO 6888-1) Other packagingDetection of staphylocoagulase 8 x 0,5 ml

55182 Lyophilized Rabbit Plasma (NF EN ISO 6888-1) Other packagingDetection of staphylocoagulase 1 x 35 ml (lyophilized) > 10,5 ml

42081 Brain-Hearth Infusion Broth (NF EN ISO 6888-1) ●Culture of fastidious bacteria 20 x 9 ml

43101 Chocolate Agar + PolyViteX ●Isolation of fastidious bacteria 2 x 10 (90 mm)

43109 Chocolate Agar + PolyViteX ●Isolation of fastidious bacteria 10 x 10 (90 mm)

41536 Chocolate Agar/PolyViteX Mixture ●Isolation of fastidious bacteria 6 x 100 ml

51026 Columbia Agar ●Isolation of fastidious bacteria 500 g

42045 Standard Methods Agar (PCA) (NF EN ISO 4833) ●Enumeration of microorganisms at 30°C in food products 6 x 200 ml

43558 Standard Methods Agar (PCA) (NF EN ISO 4833) ●Enumeration of microorganisms at 30°C in food products 2 x 10 (90 mm)

51072 Standard Methods Agar (NF EN ISO 4833) ●Enumeration of microorganisms at 30°C in food products 500 g

42100 Trypcase Soy Broth ●Culture of non-fastidious microorganisms 20 x 9 ml

42101 Trypcase Soy Agar ●Culture of nonfastidious microorganisms 20 (slope)

43011 Trypcase Soy Agar ●Isolation of non-fastidious microorganisms 2 x 10 (90 mm)

43019 Trypcase Soy Agar ●Isolation of non-fastidious microorganisms 10 x 10 (90 mm)

51019 Trypcase Soy Broth ●Culture of non-fastidious microorganisms 500 g

51044 Trypcase Soy Agar ●Isolation of non-fastidious microorganisms 500 g

41146 Trypcase Soy Broth ●Growth of non-fastidious microorganisms 6 x 100 ml

41466 Trypcase Soy Agar ●Isolation of non-fastidious microorganisms 6 x 200 ml

41467 Trypcase Soy Agar ●Isolation of non-fastidious microorganisms 6 x 100 ml

42614 Trypcase Soy Broth ●Growth of non-fastidious microorganisms 6 x 90 ml


Quality indicators

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To t a l v i a b l e c o u n t

L a c t i c a c i d b a c t e r i a

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Quality indicators

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51095 Glucose Chloramphenicol Agar - (YGC) ●(NF ISO 7954 • NF ISO 7698 • NF ISO 13681 • ISO 6611) 500 gEnumeration of yeasts and moulds

42600 Glucose Chloramphenicol Agar - (YGC)(NF ISO 7954 • NF ISO 7698 NF ISO 13681 • ISO 6611) ●Enumeration of yeasts and moulds 6 x 100 ml

42066 Sabouraud 2 Agar ●Isolation of fungi 6 x 100 ml

51020 Sabouraud 2 Agar ●Isolation of fungi 500 g

42067 Sabouraud Chloramphenicol 2 Agar ●Selective isolation of fungi 6 x 100 ml

51021 Sabouraud Chloramphenicol 2 Agar ●Selective isolation of fungi 500 g

42056 Sabouraud Gentamicin Chloramphenicol 2 Agar ●Selective isolation of fungi 6 x 100 ml

42606 Cefixime - Tellurite Mixture (NF EN ISO 16654) ●Selective isolation of Escherichia coli O157: H7 6 x 4 ml (lyophilized)

55542 Color Gram 2 Other packagingStaining of microorganisms R1= 1x 240 ml • R2= 1x 240 ml • R3= 1x 240 ml • R4= 1 x 240 ml

55545 Color Gram 2 R1 Other packagingStaining of microorganisms R1= 1 x 2 liters (color GRAM 2R1-F)




42619 D-cycloserine Supplement (NF ISO 15213 • NF EN ISO 7937) Other packagingEnumeration of Clostridium perfringens in food products 6 x 2 ml

55822 Defibrinated Sheep Blood ●Culture of fastidious bacteria. Detection of hemolysis 1 x 100 ml

55823 Defibrinated Sheep Blood ●Culture of fastidious bacteria. Detection of hemolysis 1 x 20 ml

55832 Defibrinated Horse Blood ●Culture of fastidious bacteria. Determination of hemolysis 1 x 100 ml

55833 Defibrinated Horse Blood ●Culture of fastidious bacteria. Determination of hemolysis 1 x 20 ml

55842 Horse Serum ●Germ tube test for Candida albicans 1 x 100 ml

55561 ID Color Catalase ●Detection of catalase 2 x 5 ml dropper bottles

55181 Lyophilized Rabbit Plasma (NF EN ISO 6888-1) Other packagingDetection of staphylocoagulase 8 x 0,5 ml

55182 Lyophilized Rabbit Plasma (NF EN ISO 6888-1) Other packagingDetection of staphylocoagulase 1 x 35 ml (lyophilized) > 10,5 ml

55635 Oxidase Reagent Other packagingDetection of the enzyme cytochrome oxidase 50 ampules with 0,75 ml of reagent per ampule - 1 rensable ampule crusher

55651 PolyViteX Mixture Other packagingIsolation of fastidious bacteria R1= 4 x 1 ml (lyophilized) • R2= 4 x 1 ml of solvent

55652 PolyViteX Mixture Other packagingIsolation of fastidious bacteria R1= 4 x 10 ml (lyophilized) • R2= 4 x 10 ml of solvent

73112 Slidex® Staph-Kit Other packagingSlidex Staph-Kit is a rapid latex and red blood cell agglutination test for R1= 1 x 1,7 ml Dropper bottle with red cap the identification of Staphylococcus aureus strains from culture media R2= 1 x 1,7 ml Dropper bottle with white cap

73113 Slidex® Staph-Kit Other packagingSlidex Staph-Kit is a rapid latex and red blood cell agglutination test for R1= 5 x 1,7 ml Dropper bottle with red cap • R2= 5 x 1,7 ml Dropper bottle with white capthe identification of Staphylococcus aureus strains from culture media

73115 Slidex® Staph PLUS Other packagingSlidex Staph Plus is a rapid latex agglutination test for the identification R1= 1 x 0,85 ml Dropper bottle with red cap • R2= 1 x 0,85 ml Dropper bottle with red cap •of Staphylococcus aureus strains isolated from culture media 15 disposable cards with 8 reactions fields + 2 x 50 disposable stirrers (Kit for 50 tests)

73116 Slidex® Staph PLUS Other packagingSlidex Staph Plus is a rapid latex agglutination test for the identification R1= 2 x 2,15 ml Dropper bottle with red cap • R2= 2 x 2,15 ml rapper bottle with blue cap •of Staphylococcus aureus strains isolated from culture media 5 x 15 disposable cards with 8 reactions fields (Kit for 250 tests)


S u p p l e m e n t a n d c o n f i r m a t i o n

Ye a s t a n d M o u l d s

Identificationof microorganisms

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AUTOMATED METHOD 22

VITEK® 2 COMPACT 22VITEK 2 COMPACT Reagents 24

API METHOD 25

API®/ID 32/API® 20 25APIWEB™ 27Additional accessories 28

Identification of microorganisms

AUTOMATED METHODS

VITEK® 2 CompactVITEK® 2 Compact

22

Focusing on Achieving

Excellence in Identification

VITEK® 2 Compact is the nextgeneration of the gold standard in microbial identification. How is the best made better? One compact instrument has it all: vacuum filler, cassetteloader, card sealer, readercarousel and waste container all in one small footprint.

The VITEK® 2 Compact systemis made up of multiple internalcomponents:

• Card cassette• Card filler mechanism• Cassette loading and

processing mechanism

2 • Scan barcode 3 • Identify sample1 • Enter cassette

VITEK® 2 COMPACT

Three-step secure sample tracking:

• Card sealer• Bar code reader• Cassette carousel

and incubator• Transmittance optics• Waste processing• Instrument control electronics

and firmware

Once the test cards are loaded intothe system, the instrument controlsincubation temperature, opticalreading and continually monitorsand transfers test data to the computer. When the test cycleis completed, the system automatically ejects the cards into a waste container.

• Improved performance… Expanded databases include orga-nisms unique to the industrialmicrobiology laboratory.

• Single-choice ID’s…Reduce supplemental testing

• Faster test results…Identifications are finalized inas little as two hours.

• Flexible workflow…Choice of two models to suitdifferent workloads: Virtual Cassetteor Load-and-Go.

• Improved productivity…3-step setup: prepare inoculum,scan barcode, load cassette.

• Streamlined workflow…Icon driven software providesintuitive maneuvering.

• Design improvements…New vacuum filler/sealer, internalcard barcode scanner, automaticcard waste disposal, continuousmonitoring.

• Choice of two models to suityour workload…30-test capacity, 60-test capacity.

• Simplicity…Objective technology checking inoculum turbidity withDensiCHEK.

23

The software is 21 CFR Part 11 compliant.

The VITEK 2 Compact includesmodem capability providing remotediagnostics.

Additional flexibility is available withoptions: The Networking packageallows remote functions and use ofthe laboratory's choice of hardware.OBSERVA® is a full data manage-ment system.MIMS® is a complete environmen-tal monitoring software.

SOFTWARE

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New test cardsout-perform all others:

• GN for Gram-negativeidentification.

• GP for Gram-positive identification.• YST for yeast-like organisms.• BCL for Gram-positive spore-

forming bacilli.• ANC for anaerobic bacteria and

Corynebacterium spp.• NH for Neisseria and

Haemophilus spp.• CBC* for Corynebacterium spp

and related genera.

* Consult us for availability.

VITEK® 2 Compact ReagentsVITEK® 2 Compact Reagents

Card Product number

GN Card 21341

GP Card 21342

YST Card 21343

BCL Card 21345

ANC Card 21347

NH Card 21346

CBC* Card 21348

Each card provides:

Efficiency: preinserted transfertubes; manual, external marksnot required.

Performance: 64-well cards withadvanced colorimetric substratesprovide higher accuracy.

Traceability: each card has a unique barcode identifier linking it to its sample.

Safety: each card is hermeticallysealed keeping the sample safelyinside.

Updated identification capabilities:Gram-negative fermenters and non-fermenters incorporated intoone card. Bacillus card includesan expanded database.Ability to identify Micrococcus.Ability to identify Corynebacteriaspp, Lactobacillus spp and fastidious organisms.


AUTOMATED METHODS

24

Identification cards are packaged 20per box.

The system is offeredin two models:

VITEK® 2 Compact 30:Product number 27630

VITEK® 2 Compact 60:Product number 27660

Single choice Time to result Unidentified

ANC 90% 6h 0,3%

BCL 87% 14h 1%

GN 94,3% 2-10h 0,3%

GP 93,2% 2-8h 0,3%

YST 85,2% 18h 2,2%

NH 86,3% 6h 0,8%

CBC* 85,4% 8h 0,5%

For added convenience, we provide the VITEK® 2 CompactValidation Package (Product numbers W2419 in the US; W2420 outside the US).

* Consult us for availability.


API® METHODS

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Innovation

API completely revolutionizedthe field of bacteriology.The API range introduced a standardized and miniaturizedversion of existing techniques, which,up until then, were complicated toperform and difficult to read. WithAPI, bacterial identification becamesimple, rapid and reliable.

API 20E was the first identificationsystem to be developed, combininga strip of biochemical tests and adatabase. The range was rapidlyexpanded, in collaboration with leading international reference centres in the USA, Japan,Australia and Europe…

Evolution of the technologiesenabled bioMérieux to improve itsIdentification range with the rangeID 32 strips which further increasedstandardization accuracy in theidentification of microorganisms.The innovative concept of API alsoinvolved the development of a calculation method, known asnumerical identification. This method in turn led to the design of software programs,making identification easierand more accurate.

API®/ID 32API®/ID 32

Today API continues to innovatemaking these interpretation toolsavailable anytime anywhere in theworld with access via the Internetto APIWEB.

The reference

Chosen by microbiologistsworldwide for ease-of-use andhigh-performance, the API range rapidly became the global reference for identification:

• Over 2000 scientific publicationrefer to API products.

• API systems have long beenestablished as the referencetechnique used to assess theperformance of any newidentification arriving on the market.

• API products have led to theidentification of previouslyunknown bacterial species.

• API systems are used to teach microbial identification in many higher education centres through-out the world.

The identification of allthe microorganisms

Today, the API® /ID 32 range is themost extensive range available.It includes 15 identification systemscovering all groups of bacteriaencountered in industrial microbiology laboratories: Listeria, Staphylococci, Entero-bacteriaceae and non fermentingGram negative rods, Bacillus, Lactobacillus… API can identify over 600 different species.

Reliability

Over its 30 years of existence, API has gained invaluable expe-rience in bacterial identification:around 1000 different biochemicaltests in routine use, an in depthknowledge of bacterial metabolismsand expertise in manufacturingtechniques. The scope of thisexperience makes bioMérieux the world leader in bacterial identification.In addition to regularly updating our databases and softwareapplications, we are dedicated toproducing high quality products and providing comprehensivecustomer service.

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API® 20API® 20

Reference Product name Presentation

Anaerobes20300 API 20 A 25 strips + 25 media

Campylobacter20800 API Campy 12 strips + 24 media

Corynebacteria20900 API Coryne 12 strips + 24 media

Enterobacteriaceae10100 API 10 S 50 strips20100 API 20 E 25 strips20160 API 20 E 100 strips20701 RapiD 20 E 25 strips

Gram Negative Rods20050 API 20 NE 25 strips + 25 media

Listeria10300 API Listeria 10 strips + 10 media03700 Rapidec® Lmono 20 tests

confirmation of Listeria monocytogenes identification carried out on chromID™ OAA

Neisseria - Haemophilus10400 API NH 10 strips + 10 media

Staphylococci20500 API Staph 25 strips + 25 media

Streptococci20600 API 20 Strep 25 strips + 25 media

Yeasts20210 API 20 C AUX 25 strips + 25 media10500 API Candida 10 strips + 10 media

Other50300 API 50 CH 10 strips50430 API 50 CHB/E medium 10 x 10 ml50410 API 50 CHL medium 10 x 10 ml25200 API ZYM 25 strips88818 Biotype 100 12 strips88819 Biotype Medium 1 12 bottles88820 Biotype Medium 2 12 bottles

RAPIDEC strips03300 RAPIDEC Staph 30 tests

API IDENTIFICATION


Anaerobes32300 Rapid ID 32 A 25 strips

Enterobacteriaceae32400 ID 32 E 25 strips32700 Rapid ID 32 E 25 strips

Gram Negative Rods32100 ID32 GN 25 strips + 25 media

Staphylococci32500 ID32 STAPH 25 strips

Streptococci32600 Rapid ID32 STREP 25 strips

Yeasts32200 ID32 C 25 strips + 25 media

ID 32/RAPID ID 32

API® METHODS


API® METHODS

APIWEBAPIWEB™Anytime, anywhere


40011 APIWEB 1 license

27

Simplified interpretationfor every laboratory

bioMérieux once again gives a newdimension to its API® /ID 32 rangeby making identification availableto everyone, at anytime, andanywhere.APIWEB, a tool which encompasses the technologies of today and the future, is theessential complement to manualidentification.

User Friendly

• Internet platform• Easy to use, integrated intuitive interface using Internet navigation tools

• Available 7 days a week, 24 hours a day

• Reading assistance foreach identification strip

High performance

APIWEB, contains all the API® /ID 32databases which are regularlyupdated.Containing more information thanAPI analytical profile indices, APIWEB interprets a great numberof profiles enabling the identifica-tion of 600 species of bacteriaand yeasts. It is a reliable, state ofthe art interpretation tool.

Each identification is associatedwith a complete report:

• species proposed;• comments reflecting the reliability of the identification, selected after the calculation of two indices:

- the identification percentage (probability of species identification) - and in the typicity index (typical character of theprofile studied);

• complete biochemical profile;• additional information and tests.

Security

• Secure website, personalized access codes.

• Data encoding ensuring theintegrity and confidentiality of your results.

• Highly reactive maintenance tool.

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API® Additional reagentsand accessories

API® Additional reagentsand accessories


20120 API 20 E Reagents 7 ampoules

50120 API M Medium 10 x 5 ml

20070 API NaCl 0.85% Medium (2ml) 100 ampoules



50110 API OF Medium 10 x 5 ml

70700 API Suspension Medium (2ml) 100 ampoules



70510 BCP 1 ampoule

70610 Sterile swabs 100

70520 EHR 1 ampoule

70562 FB 2 ampoules

70100 Mineral oil 1 x 125 ml

70542 James 2 ampoules

70900 McFarland Standard 6 standards

70491 NIN 2 ampoules

70442 NIT1 + NIT2 2 x 2 ampoules

70760 10µl sterile loops 50 x 10

70200 12 ampoule rack 1

70901 Ampoule protector 1 x 3

70250 Sterile Pipette (5ml) 400

70492 PYZ 2 ampoules

70402 TDA 2 ampoules

70422 VP1 + VP2 2 x 2 ampoules

70530 XYL 2 x 5 ml

70380 Zn 2 x 10g

70494 ZYMA 2 ampoules

70493 ZYMB 2 ampoules

ADDITIONAL REAGENTS AND ACCESSORIES

API® METHODS

Microbial Genotyping

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DIVERSILAB® 22

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DiversiLab®DiversiLab®

Microbial Genotyping

Reference Product name

270600 DDLL AAcciinneettoobbaacctteerr KKiitt

270601 DDLL AArrcchhaaeeaa KKiitt

270602 DDLL AAssppeerrggiilllluuss KKiitt

270603 DDLL BBaacciilllluuss KKiitt

270633 DDLL BBaacctteerriiaall KKiitt

270604 DDLL BBiiffiiddoobbaacctteerriiuumm KKiitt

270605 DDLL BBoorrddeetteellllaa KKiitt

270606 DDLL BBuurrkkhhoollddeerriiaa KKiitt

270607 DDLL CCaammppyylloobbaacctteerr KKiitt

270608 DDLL CCaannddiiddaa KKiitt

270609 DDLL CClloossttrriiddiiuumm KKiitt

270610 DDLL CC..ppeerrffrriinnggeennss KKiitt

270611 DDLL EEnntteerroobbaacctteerr KKiitt

270612 DDLL EEnntteerrooccooccccuuss KKiitt

270613 DDLL EEsscchheerriicchhiiaa KKiitt

270614 DDLL HHaaeemmoopphhiilluuss KKiitt

270615 DDLL KKlleebbssiieellllaa KKiitt

270616 DDLL LLaaccttoobbaacciilllluuss KKiitt

270617 DDLL LLeeggiioonneellllaa KKiitt

270618 DDLL LLiisstteerriiaa KKiitt

270650 DDLL MMoolldd KKiitt

270619 DDLL MMyyccoobbaacctteerriiuumm KKiitt

270620 DDLL MMyyccooppllaassmmaa KKiitt

270621 DDLL MM.. ttuubbeerrccuulloossiiss KKiitt

270622 DDLL NNeeiisssseerriiaa KKiitt

270623 DDLL PPrrooppiioonniibbaacctteerriiuumm KKiitt

270624 DDLL PPsseeuuddoommoonnaass KKiitt

270632 DDLL SSaacccchhaarroommyycceess KKiitt

270625 DDLL SSaallmmoonneellllaa KKiitt

270626 DDLL SSeerrrraattiiaa KKiitt

270627 DDLL SShhiiggeellllaa KKiitt

270628 DDLL SSttaapphhyyllooccooccccuuss KKiitt

270629 DDLL SStteennoottrroopphhoommoonnaass KKiitt

270630 DDLL SSttrreeppttooccooccccuuss KKiitt

270652 DDLL YYeeaasstt KKiitt

DIVERSILAB® FINGERPRINTING KITS (48 TESTS)


270670 DDLL LLaabbCChhiipp KKiitt ((2255 cchhiippss)) 332255 tteesstt

270678 DDLL LLaabbCChhiipp rreeaaggeennttss 332255 tteessttss

DIVERSILAB REAGENTS


270709 DDLL AAggiilleenntt 22110000 BBiiooaannaallyyzzeerr

DIVERSILAB ANALYSER

SSppeecciiffiiccaattiioonnss:: wweeiigghhtt == 1100 kkgg ((2222 llbbss))

DDiimmeennssiioonnss:: WW 116622 XX HH 441122 XX DD 229900mmmm ((WW 6644 XX HH 1166..22 XX DD 1111..44 iinncchheess))

CCoonnttaacctt yyoouurr bbiiooMMéérriieeuuxx rreepprreesseennttaattiivvee ffoorr mmoorree ddeettaaiillss aanndd pprroodduucctt aavvaaiillaabbiilliittyy..

The DiversiLab system provides best in class genotypiccharacterization of bacteria, yeast and mold, providingsame day strain typing for real time investigations.Meet your expectations for food safety, product controland investigations - rapidly locate the source and stop the spread of further contamination.The system automates and standardizes a traditionallycomplex process while increasing consistency, reproducibility and ease of interpretation.The DiversiLab system has wide applications in food, agricultural, and environmental control. Software is dynamic, with secure reports and 21CFR11 compliance.The DiversiLab system complements bioMerieux's complete offer in food safety and product control.

Environmentalcontrol

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airIDEAL® 3PTM 32

COUNT-TACT ® RANGE 33

READY-TO-USE MEDIA/Legionella 34

32

Environmental control

airIDEAL® 3PTMairIDEAL® 3PTMThe complete, practical and reliable solutionfor aerobiocontamination control

Reference Presentation

96 302 airIDEAL equipped with 90 mm diameter sampling grids

96 303 airIDEAL equipped with 65 and 70 mm diameter sampling grids

96 309 90 mm diameter additional sampling grids

96 304 65/70 mm diameter additional sampling grids

96 308 Aluminium tripod with ball joint and adapter

Optimized performancewith the bioMérieux pre-poured media range

bioMérieux is committed toenvironmental controls.Aerobiocontamination controldepends on the air samplerperformance and on the qualityof the culture media used.A wide range of ready-to-usestandard and irradiated culturemedia, developed and manufacturedin compliance with our QualitySystem, optimize airIDEAL’sperformance.

Conformity withstandards andrecommendations

The airIDEAL® aerobiocollectoroperates according to the impactionprinciple recommended by the ISOstandard 14698. The control of airintake and speed of impactensures the collection of viable

and non-altered particles.The instrument is CE, UL and CSAmarked (detailed certificatesdelivered with airIDEAL® 3PTM).

Greater user comfort

• Easy-to-use- Easy-to-handle and light-

weight (1.2 kg), its ergonomic,ambidextrous handle meansit can be held with only onehand.

- User-friendly software:storage of sampling parameters. An audible and visual alarmat the end of the cycle. It ispossible to program start-upwithout the user’s presence(1 to 60 minutes).

• Robust: monoshell made ininjected ABS plastic.

• Universal, 2 versions are availa-ble to adapt to the diameters ofcommercialized agar media plates

(65/70 and 90 mm diameter).

• Easy-to-maintain and resistantto disinfectants with its smoothsurface and no dead angles.

• Sampling grids autoclavableat 134 °C for 18 minutes.

Greater controlsecurity

Precise, metrologically tested (*)

air intake rate.

4-hour autonomy with an NiMHbattery, specially designed toprevent the progressive decreasein battery capacity.

The instrument’s design alsoguarantees continuous new airintake (intake air is evacuated onthe opposite side to air intake).

(*) by the external organism CETIAT:Technical Center for the Aeraulic andThermal Industries (www.cetiat.fr)

With the aerobiocollector airIDEAL® 3PTM, bioMérieux iscommitted to the microbiologicalcontrol of air. airIDEAL® 3PTM

ensures the effective measurementof aerobiocontamination.

Air quality control is a keyprocess in the Quality systemof agri-food industries.

This control must meet everhigher requirements defined byincreasingly strict regulations.

33

Count-Tact™ RangeCount-Tact® Range


43501 Count-Tact ® Agar (ISO 14698-1 • ISO 18593 • NF V 08-037) ●For the monitoring of biocontamination of surfaces in hospital 2 x 10 (55 mm)and industrial environments (4 neutralizers)

43582 Count-Tact ® GTS/TSA Agar (ISO 14698-1 • ISO 18593 • NF V 08-037) ●For the monitoring of biocontamination of surfaces in hospital 2 x 10 (55 mm)and industrial environments

43781 Count-Tact ® VRBG Agar (ISO 18593 • NF V 08-037) ●For the detection and enumeration of Entero- 2 x 10 (55 mm)bacteriaceae on surfaces in the environment of agri-food industries

43791 Count-Tact ® YGC Agar ●For the detection and enumeration of yeast and moulds 2 x 10 (55 mm)on surfaces in the environment of agri-food industries

96301 Bi-Box Other packagingSterile case for collection of Count-Tact plates for environmental controls 1 x 10 Bi-Box

96311 Bi-Box 90 Other packagingSterile case for collection of 90 mm plates for environmental controls 1 x 10 Bi-Box 90

96300 Count-Tact ® Applicator Other packagingApplicator for Count-Tact plates 1 applicator + 1 control certificatestandardization of surface sampling

Tube Bottle Plate DehydratedmediaTo t a l v i a b l e c o u n t

E n t e r o b a c t e r i a c e a e


E q u i p m e n t

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IRO

NM

ENTA

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43013 Legionella BCYE with L-Cysteine (NF T 90-431 • ISO 11731) ●Medium for confirmation of suspected Legionella 1 x 10 (90 mm)

43023 Legionella BCYE without L-Cysteine (NF T 90-431 • ISO 11731) ●Medium for confirmation of suspected Legionella 1 x 10 (90 mm)

43031 Legionella GVPC Agar (NF T 90-431 • ISO 11731) ●Selective isolation of Legionella 2 x 10 (90 mm)

43032 Legionella GVPC Agar (NF T 90-431 • ISO 11731) ●Selective isolation of Legionella 10 x 10 (90 mm)

73120 Slidex Legionella kit Other packagingIdentification of L.pneumophila and L.anisa from environmental waters 50 tests

43596 Sabouraud Dextrose Chloramphenicol Agar (ISO 14698-1 • ISO 18593 • NF V 08-037) ●Agar for the enumeration of yeasts and moulds 2 x 10 (90 mm)

43801 QUANTISWAB™ Other packagingSurface Sampling with swabs and a transport tube 200 swabs


A c c e s s o r i e s

L e g i o n e l l a



Microbial detection

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35

AUTOMATED METHOD 36

BacT/ALERT® 3D Microbial Detection System 36BacT/ALERT® Reagents 38

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Microbial detection

AUTOMATED METHODS

BacT/ALERT® 3D Microbial Detection SystemBacT/ALERT® 3D Microbial Detection System

Fast, reliable, sensitive, andproblem-free microbial spoilagedetection is possible and is inroutine use in several well-knownaseptic production facilities throughout the world.They are using bioMérieux’sBacT/ALERT® 3D MicrobialDetection System.

With the introduction of theBacT/ALERT, rapid testing andall the benefits it can providehave arrived. BacT/ALERT can beimplemented with relative easeinto any laboratory performingpresence/absence testingof spoilage microorganisms andeffectively improve the quality oftesting for many product types.The BacT/ALERT 3D also reduceshands-on time and eliminates

When a bottle is placed intoa cell in the detection unit, a small light-emitting diode(LED) exposes the sensor to a red light beam. The reflected-light is collected every ten minutes by a photodiode builtinto each cell, where it istransformed and amplifiedbefore being transmitted tothe computer system forinterpretation.

The computer software generatesa growth curve based on production of CO2 and othermetabolic by-products that are

DETECTION PRINCIPLE

dissolved in the culture media foreach sample. Through the useof complex algorithms, theBacT/ALERT 3D MicrobialDetection System discriminatesbetween constant CO2 and other metabolic by-products production and an acceleratedrate of CO2 and other metabolicby-products production causedby growing microorganisms.

As additional CO2 is generatedwithin the culture bottle, thebuilt-in sensor changes froma grayish color to a lighter yellowcolor. Even the slightest color

36

false positives, re-testing, lengthysample pre-incubation andinstrument standardization byproduct. There is no longer theneed to run batch controls or todevelop baselines to compensate

for background readings.All of this can be accomplishedwithout sacrificing accuracy andreliability.

Wide range ofproducts can be tested usingBacT/ALERT® 3D

• Different product types can berun simultaneously.

• Culture bottles available for highand low acid products.

• Each bottle is compared to itsprevious readings, not pre-setthresholds determined for eachproduct.

• Measurements not taken directlyfrom media/product mixture.

• No false positives due to initial pHor initial brix/acid ratio of product.

• No need for negative controls runwith each product to establishcutoffs.

• Results easily verified by wet prepor Gram stain performed frombottle.

BacT/ALERT® reduceshands-on time

• Sample bottles already containappropriate media for high or lowacid products.

• Bottles are bar-coded for easydata entry and bottle loading.

• Barcodes permit ease of sampleidentification and tracking.

• No pre-treatment of sample toremove non-microbial backgroundinterference.

• Once inoculated, the bottle isloaded into the instrument andrequires no additional handlingduring testing.

• May reduce staffing requirementsor allow increased productioncapacity without additionalpersonnel.

Large sample volumesoften eliminate the need for lenghtypre-incubations

• Sample size up to 10-20 mldepending on the bottle typeused.

• Large sample volumes provide for20-100 fold increase in sensitivity.

• Media developed to facilitategrowth in high acid or low acidproducts.

• Detection level as low as1 organism per sample.

• Instrument continuouslyincubates, agitates and monitorseach sample.

• Alerts lab as soon as bottle isdetected as positive.

• Spoilage organisms generallybecome positive in 24-48 hoursor less.

Standardization

• No need for negative controlsto set baseline or threshold fordifferent product types.

• Each bottle must pass strict QCprocedures during and aftermanufacture.

• Instrument is automaticallyQC’d regularly to ensure accuratereadings.

• Each bottle is compared to itsprevious readings, not pre-setthresholds determined foreach product.

• Sophisticated computeralgorithms to detect growthanalyze changes in bottlereadings.

change is detected by theinstrument and immediatelytransmitted to the BacT/ALERTcomputer. Since each sampleis compared to its own past performance rather than a preset threshold, true positivesare detected rapidly without anincrease in false positives.

When a sample is determinedpositive, the computer systemalerts the user and a lightappears at the cell of the bottleto be removed.

37 MIC

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AUTOMATED METHODS

BacT/ALERT® ReagentsBacT/ALERT® Reagents

The BacT/ALERT system utilizesdisposable culture bottles - in both aerobic and anaerobicmedia formulations - for testing avariety of low and high acid foodand beverage products.Each self-contained BacT/ALERTculture bottle is sterile andcontains an internal colorimetricsensor. Caps and labels arecolor-coded for easy identification.

All bottles feature a bar code label,which assigns a unique ID numberand identifies it by media type.While providing a convenienttracking system, these labels alsoreduce data entry and clericalerrors. With the bar code reader,bottles can be quickly and easilylogged into the system, andplaced directly in the instrumentfor monitoring.

38

Acetobacter pasteurianus ATCC® 12879

Alicyclobacillus acidoterrestris ATCC 49025

Bacillus coagulans ATCC 7050

Leuconostoc mesenteroides ATCC 10830

Lactobacillus plantarum ATCC 14917

Pediococcus pentosaceus ATCC 33316

Saccharomyces cerevisiae ATCC 36375

Zymomonas mobilis ATCC 31821

Aspergillus niger ATCC 16404

Byssochlamys fulva ATCC 10099

Paecilomyces lilacinus ATCC 90461

Zygosaccharomyces bailii ATCC 60484

Bacteroides vulgatus ATCC 8482

Escherichia coli ATCC 8739

Staphylococcus aureus ATCC 6538

Clostridium sporogenes ATCC 11437

Clostridium sporogenes ATCC 19404

Propionibacterium acnes ATCC 11827

Bacillus subtilis ATCC 6633

Pseudomonas aeruginosa ATCC 9027

Staphylococcus aureus ATCC 6538

Escherichia coli ATCC 8739

Candida albicans ATCC 10231

Aspergillus niger ATCC 16404

Micrococcus luteus ATCC 9341

BacT/ALERT® i LYM 259788

BacT/ALERT i LYM culture bottles provide a culturemedium with suitable nutritional and environmentalconditions for the growth and detection of a varietyof microorganisms that may be present in high acidfood and beverage products.

BacT/ALERT® i NST 259785

The BacT/ALERT i NST culture bottles provide culturemedium with suitable nutritional and environmentalconditions for the growth and detection of a varietyof anaerobic microorganisms that may be presentin processed or aseptically prepared products.

BacT/ALERT® i AST 259786

The BacT/ALERT i AST culture bottles providesa culture medium with suitable nutritional and environmental conditions for the growth and detection of a variety of aerobic microorganisms that may be present in processed or aseptically prepared products.

Examples of microorganisms detected



bioMérieux SA69280 Marcy l’EtoileFranceTel: (33) 04 78 87 20 00Fax: (33) 04 78 87 20 90

www.biomerieux.comwww.biomerieux-industry.com05

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