microbiology charts

7/30/2019 Microbiology Charts

1/17

1. Sheep Blood agar

2. Bacteroides Bile-esculin agar

3. Bile esculin agar

4. Brucella base agar

5. Blood with kanamycin and vancomycin

6. Phenylethyl alcohol agar (aerobic and anaerobic)

7. Bordet-gengou Blood agar

8. Buffered charcoal-yeast extract agar

9. Burkholderia cepacia selective agar

10. Campylobacter blood agar

11. Cefsulodin-Irgansan-Novobiovin agar

12. Chococlate agar

13. Chromagars

14. Simmons Citrate agar

15. Columbia agar with antibiotics

16. CTA sugars

17. Egg Yolk Agar

18. Eosin Methylene Blue Agar

19. Hektoen Enteric Agar

20. Lowenstein-Jensen medium21. Macconkey agar

22. Macconkey sorbitol agar

23. Mannitol salt agar

24. Modified Thayer Martin agar

25. Mueller Hinton agar

26. Mueller hinton agar with 4% NaCl and 6 ug Oxacillin

27. New York City Medium

28. Pseudomonas cepacia agar


2/17

29. Regan-Lowe medium

30. Salmonella-Shigella agar

31. Thiogylcolate broth

32. Thiosulfate citrate bile salts sucrose agar

33. Tinsdale agar

34. Triple Sugar Iron (TSI)

35. Kligers Iron Agar (KIA)

36. Trypticase Soy Agar

37. Urea Broth

38. Urea Agar

39. Xylose-Lysine-Desoxycholate Agar

40. Vaginalis Agar


3/17

Type

Enriched, Nonselective, and Differential (Detection of hemolysis)

Selective and Differential


Nonselective nutrient medium

Enriched and Selective

Selective

Enriched. May be made selective by adding antibiotics

Nonselective and Enriched


Selective


Enriched and Nonselective

Nonselective and Differential

Differential

Nonselective

Differential




NonselectiveSelective and Differential

Selective


Selective

Nonselective

Nonselective

Selective

Selective


4/17

Selective


Nonselective



Differential

Differential

Nonselective

Selective

Differential




5/17

Organisms That Will Grow

Fastidious/Nonfastidious organisms. Ex: Staph, Strep, GPR, Most Neisseria species, etc.

Isolation and Presumptive ID ofBacteroides fragilis group

Isolation of Enterococcus species

Cultivaton of many organisms

Isolation of obligately anaerobic organisms

Isolation of gram (+) organisms. Inhibition of gram (-) organisms

Isolation ofBordetella pertussis and B. parapertussis

Isolation ofLegionella. Will also support the growth ofBordetella, Brucella, and Francisella tularensis

Isolation of B. cepacia

Isolation of Campylobacter jejuni subspecies jejuni and C. coli

Isolation of Yersinia enterocolitica, which ferments mannitol and forms bullseye colonies. Also used to isolate Aeromonas from stool speci

Cultivation of fastidious organisms, including Haemophilus, Neisseria gonorrhoeae, and Brucella

Isolation of MRSA

Gram (-) bacteria ability to utilize citrate

Cultivation of many organisms

Determines if organisms can ferment various carbs: maltose, lactose, sucrose. Differentiates Neisseria and Moraxella species

Identifiation of certain anaerobic isolates

Isolation of nonfastidious GNR. Inhibition of gram (+) organisms. E. coli (Strong lactose fermenter), Shigella and Salmonella (Non-lactose f

Isolation ofSalmonella and Shigella. Inhibition of gram (+) organisms and some gram (-).

Cultivation of MycobacteriaInhibition of Gram (+) organisms. Isolation of many nonfastidious GNR, Ex. Enterics.

Isolation of E. coli H0157:H7

Isolation of S. aureus, which uses mannitol and turns medium yellow. Inhibition of most gram (-) and many gram (+) organisms.

Isolation of Neisseria gonorrhoeae

Antimicrobial Susceptibility Tests

Isolation of Neisseria gonorrhoeae

Isolation of Neisseria species

Isolation ofBurkholderia cepacia (Previously known as Pseudomonas cepacia)


6/17

Isolation of Bordetella pertussis and B. parapertussis

Isolation of Salmonella and Shigella. Inhibition of gram (+) organisms and some gram (-).

Supports the growth of aerobes, anaerobes, and facultatives

Isolation of Vibrio. Inhibition of many gram (+) and gram (-) organisms.

Isolation of Corynebacterium diphtheriae

Contains lactoe, sucrose, and glucose. Differentiates enteric organisms.

Differentiates gram (-) enteric bacilli.

Cultivation of fastidious and nonfastidious organisms. May be used as base for blood agar

Tests for the presence of the enzyme urease

Differentiates members of the Enterobacteriaceae group

Isolation of Salmonella and Shigella. Inhibition of gram (+) organisms and some gram (-).

Isolation of Gardnerella vaginalis, which is B-hemolytic on human blood.


7/17

Special Comments

Nutritional base

Inhibitors: Gentamicin and Bile

Bile salts and Esculin

Nutrients (Blood may be added)

Inhibits gram (+) organisms

Inhibitor: Phenylethyl alcohol

Glycerol, potato infusion, and blood. Nutrients. Also inactivate toxic substances.

Charcoal: Removes toxic substances. Nutrients: Yeast etract, cysteine, iron salts, and alpha-ketoglutarate

Isolated from respiratory secretions

Nutrient base, inhibitors: antimicrobial agents, blood may be added

Inhibitors: Cefsulodin, irgasan, novobiocin, bile salts, crystal violet. Carb: Mannitol

Nonfastidious organisms will also grow. Nutritional base, heated RBCs or other supplements

None

None

Nutrient, blood/antibiotics may be added

None

None

Inhibitors and pH indicators. Contains carbohydrate: lactose.

Inhibitor: Bile salts. pH indicators: Acid fuchsin and bromthymol blue. Carbs: Lactose, sucrose, salicin. H2S system.

Eggs, Malachite greenInhibitors: Bile salts and crystal violet. pH indicator: Neutral red. Carb: Lactose.

None

Inhibitor: 7.5% salt. pH indicator: Phenol red. Mannitol

None

Supplements may be added: Salt, cations (broth), sheep or lysed horse blood

None

None

Contains antimicrobial agents


8/17

None

Inhibitors: Bile salts, citrate, and brilliant green. pH indicator: Neutral red. Carb: Lactose. H2S system.

Reducing agents: Thioglycollate, cystine, sodium sulfite. Agar: Reduces O2 diffusion.

Inhibitors: Citrate, bile, and high pH. pH indicator: Bromthymol blue. Carb: Sucrose. H2S system.

Tellurite: inhibitors many organisms

None

None

Contains: Digested soybeans and casein

None

None

Inhibitor: Bile salts. pH indicator: Phenol red. Carbs: Lactose, sucrose, xylose. Lysine. H2S system

Contains: Nutrients, human blood


9/17

Bacitracin susceptibility

CAMP test

Hippurate Hydrolysis

PYR hydrolysis

Optichin susceptibility

Bile esculin hydrolysis

Salt-Tolerance Test

X and V Factor Requirement

Oxidase

Spot Indol

Catalase

Coagulase

Hydrogen sulfide production

Urease

Motility

Vibriotstatic compound, O/129 disc test

ONPG

Deoxycholate

Cefinase


10/17

Purpose/Principle

Some organisms are suseptible to 0.04 units of bacitracin. Inoculate agar for confluent growth; add dis

GBS make CAMP factor. S aureus makes B-lysin. Hemolysis enhanced when CAMP factor meets B-lysin.

Hippurate ---(Hippuricase)---> Sodium benzoate + glycine. Detect either benzoate or glycine. Benzoate

Chemical reaction - detects B-naphthylamine

Inoculate agar plate for confluent growth with isolate. Place disk onto inoculate area. Incubate plate;

40% bile inhibits many organisms. Esculin ---> Esculetin + Glucose. Esculetin + Ferric ions --> Black

6.5% salt inhibits many organisms. Inoculate broth with organism

Test organism inoculated onto trypticase soy agar or Mueller-Hinton agar, which lack X and V factors.

Chemical Reaction - Detects indophenol

Rub organism onto reagent-impregnated filter paper.

Place organism on slide and add drop of H2O2. Detect bubbles

Prepare saline suspension, mix in drop of plasma

H2S + Iron or lead ----> Black

Medium becomes alkaline as ammonia is produced

Stab organism into semisolid agar deep

Inoculate agar for confluent growth, add disks, incubate plate and measure zone

Chemical reaction - Yellow color change in positive reaction

Added to agar in order, visible pitting in the agar -- positive reaction

Beta-lactamase detection


11/17

Pos. Rxn

Susceptible: Any zone

Enhanced hemolysis

Benzoate: Precipitate, Glycine: Blue

Red

Susceptible: Zone > Cutoff

Black

Turbidity or acid pH

Growth around disk

Dark purple or Blue-black

Blue-green

Bubbles

Cell clumping

Black

Red/pink

Diffuse haze of growth throughout

Any zone

Yellow

Pitting in agar

Yellow to Red color change


12/17

Pos. Organism

Micrococcus, GAS

GBS

GBS, Listeria, C. jejuni

GAS, Enterococcus, Other gram (+) bacteria

S. pneumoniae

Enterococcus, GDS, Listeria

Enterococci

X only: H. ducreyi, V only: H. parainflu and H parahaemo., X and V: H. influ and H. haemo.

Neisseria, Moraxella, Pseudomonas, B. cepacia, Vibrio, Aeromonas, and others

E coli, P vulgaris, Morganella, Vibrio, Aeronomas, Plesiomonas, and others

Staph, Micrococcus, Corynebacterium, Many Bacillus, and others

S aureus

Edwardsiella, Salmonella, Proteus

K pneumoniae, Proteus, Morganella, Most Brucella, H pylori, and others

Vibrio and Campylobacter (Darting), Bacillus, Many enterics, Aeromonas, and others

Susceptible: Plesiomonas, Most V. cholerae

E coli, Citrobacter, K pneumoniae, Enterobacter, S marcescens

Enterics

N gonorrhoeae, H influenzae, M catarrhalis, Enterococcus, Staph, Anaerobic bacteria


13/17

Neg. Rxn

Resistant: No zone

No enhanced hemolysis

Benzoate: No precipitate, Glycine: No color

No color change

Resistant: No zone

No color

No turbidity or pH change

No growth around disk

No color change

No color

No bubbles

No clumping

No color

Yellow

Growth only near stab line

No zone

Colorless

No pitting in agar

No color change


14/17

Neg. Organism

Staph, Stomatococcus, GBS, Other B-strep

Groups A, C, D, F, and G Strep, Enterococci

Groups A, C, F, and G Strep, Enterococci, Helicobacter

Other Strep

Other Strep

Other Strep

GDS not enterococci, Other Strep

All other bacteria

Enterics, S. maltophilia, Acinetobacter, Gardnerella

Salmonella, K pneuoniae, Enterobacter, and others

Clostridium, Fusobacterium, and others

S saprophyticus, S epidermidis, Many other Staph species, Micrococcus

E coli, Shigella, Klebsiella, Enterobacter, Serratia

E coli, Shigella, Salmonella, S marcescens, P multocida

Corynebacterium, Lactobacillus, Shigella, Klebsiella, Brucella, and others

Resistant: Aeromonas

Salmonella, Proteus, Providencia, Morganella

Gram positive bacteria

Non-B-lactamase bacteria


15/17

Urinary tract

Genitals and STDs

Upper Respiratory Tract

Lower Respiratory Tract

Ocular

Skin and Soft Tissue (what about bone?)

Gastrointestinal + Agents of possible food poisoning

Central Nervous System

Circulatory (blood)


16/17

Pathogens

E coli, Other Enterobacteriaceae, S saprophyticus, P aeruginosa

N gonorrhoeae, C trachomatis, T pallidum, H ducreyi

S pyogenes, H influenzae

S pneumoniae, H influenzae, S aureus, M tuberculosis

S aureus, N gonorrhoeae, C trachomatis

B burgdorferi, R ricketsii

V cholerae, Salmonella, C jejuni, Shigella, C difficile, S aureus

C botulinum, C tetanus, N meningitidis

S pyogenes


17/17

Disease Associated Areas Affected

UTI, Pyelonephritis Bladder, Ureters, Kidneys

Gonorrhea, Chlamydia, Syphillis, Chancroid Genitals

Strep throat, Influenza Throat, Lungs

Pneumonia, Tuberculosis Lungs

Conjunctivitis (Infectious and Neonatal), Trachoma Eyes

Lyme disease, Rocky Mountain Spotted Fever Skin

Cholera, Salmonella food poisoning GI Tract

Botulism, Tetanus, Meningitis CNS

Sepsis, Endocarditis Heart, Blood Vessels

microbiology charts

Documents