Microbiology Laboratory

Group # 2 2G-PH April 6, 2016Members: Alodia O. Castillo Paul D. Chiong Alison A. Claveria Regina DG. De Guzman Jehhan N. Decampong Aryssa Gem D. Dela Cruz Angelica B. Dela Rosa

I. Identification of Unknown From A Chemical Sample

Observations & Results

Name of the organism: Escherichia coli

A. Plated Media

Description of the colony Interpretation of the results

1. EMB Colorless Non-lactose fermenters

2.MacConkey Pink Lactose fermenters

3.SSA Colorless Non-lactose fermenters

Before inoculation:

EMB MacConkey SSA

After inoculation:

EMB MacConkey SSA

B. Tubed Media

Description of the Colony Interpretation of Results

1. TSI There is formation of bubbles and displacement of medium.

AA with gas production

2. LIA Purple KK (alkaline)

3. SIM Hydrogen Sulfide (-),Indole (+)

Motile

4. SCA Prussian Blue Positive (+)

5. Trypton Blue (TB) Red/Fuchsia ring Positive (+)

6. Methyl Red (MR) Red Positive (+)

7.Voges Proskauer (VP) Yellow Negative (-)

8. Urea Broth Salmon/Orange Negative (-)

Before inoculation: After inoculation:

TSI LIA TSI LIA

Before inoculation:

SIM SCA TB MR VP UB

After inoculation:

SIM SCA TB MR VP UB

Microbiology Laboratory

Group # 2 2G-PH April 6, 2016Members: Alodia O. Castillo Paul D. Chiong Alison A. Claveria Regina DG. De Guzman Jehhan N. Decampong Aryssa Gem D. Dela Cruz Angelica B. Dela Rosa

II.Pathogenicity Test For Staphylococci Mannitol Fermentation Test

Observations & Results:

MSA Organism A Organism B

Description of the Colony Yellow colonies Pink colonies

Interpretation of the Results Mannitol fermenters Non-mannitol fermenters

Before Inoculation: After Inoculation:

MSA MSA (A) MSA (B)

Name of Organism: Name of Organism:

Staphylococcus aureus Staphylococcus epidermidis