microbiology practical i the following slides were contributed by your fellow bio 251 students…...
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![Page 1: Microbiology Practical I The following slides were contributed by your fellow BIO 251 students… Use side “ ” key to view slides…](https://reader036.vdocument.in/reader036/viewer/2022062421/56649d4a5503460f94a26f26/html5/thumbnails/1.jpg)
Microbiology Practical I
The following slides were contributed by your fellow BIO 251 students…
Use side “” key to view slides…
![Page 2: Microbiology Practical I The following slides were contributed by your fellow BIO 251 students… Use side “ ” key to view slides…](https://reader036.vdocument.in/reader036/viewer/2022062421/56649d4a5503460f94a26f26/html5/thumbnails/2.jpg)
Lab 1: Eukaryotes and Microscopy
Know the parts of the microscope…
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Lab 1: Eukaryotes and Microscopy
Paramecium caudatum
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Lab 1: Eukaryotes and Microscopy
Euglena gracilis
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Lab 1: Eukaryotes and Microscopy
Amoeba proteus
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Lab 1: Eukaryotes and Microscopy
Fungus – Saccharomyces cerevisiae
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Lab 1: Eukaryotes and Microscopy
Cyanobacteria ~ Nostoc
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~Bacteria slides~
Micrococcus luteus
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~Bacteria Slides~
Bacillus subtilis
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~Bacteria Slides~
Bacillus anthracis
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~Bacteria Slides~
Rhodospirillum rubrumSorry not in focus…
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~Bacteria Slides~
See other file for Gram Stain, Endospore, Acidfast, and Capsule Staining…
[ Adobe Acrobat of Gram and Endospore ]
Some Endospore Staining ~
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Streak Plate:
Know colony characteristics (color, elevation, margin, etc.)
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Spread Plate vs. Streak Plate:
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Streak Plate:
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Spread Plate:Mixed – Bacillus subtilis & Serratia marcescens
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Practical I Unknown Tests:
~ Simple Stain~ Gram Stain~ Endospore Stain~ Acid-Fast Stain (Demo)~ Capsule Stain (Demo)~ Motility (Hanging Drop, Wet Mount, Soft Agar Deep)
~ Obligate Aerobe, Facultative Anaerobe, Aerotolerant Anaerobes, Microaerophiles (Gas Pak, Eugon Agar, Thioglycollate Broth)
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Gas Pak Anaerobic vs. Aerobic:
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Dilution Problems:
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Dilution Problems:
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Bacterial Growth Curve:Make sure you know how to:
Use a spectrophotometer to measure absorbance.
Produce a log graph of the calculations.
Calculate generation time from a growth curve.