microfluidic construction kit thinxxs 30mar2011 · fields of microfluidics, - to test fluidic...

microfluidic construction kit

modular conceptbasic platform technology

plug and play

With our microfluidic construction kit you

will significantly accelerate the development

of your own Lab-on-Chip products. The modular

concept and the basic platform technology allow

- economical access to the

fields of microfluidics,

- to test fluidic single functions

and parameter studies,

- to generate complete protocols and

- to develop and verify your

product specifications.

The construction kit includes:

1 microplate assembly platform

5 snake mixer slides

5 fluid splitter slides

1 set of micro connectors, plugs, and tubing

assembly platform

standard components

Microplate assembly platforms (MTF0204)

are made of PC and allow to mount up

to four microfluidic construction kit slides.

The standard microplate platform is colored

black, but of course we realize customized

platforms (e.g. in terms of corporate design).

(all dimensions in mm)

MTF0204

4.5 6.5

SMS0104

snake mixer slide

The SMS0104 features a total of four passive

flow-through mixers, which intermix smallest

liquid volumes within milliseconds. The mixers

are integrated on a microscope slide and cover

a wide range of potentially interesting flow rates

or viscosities.

The slide is made of cyclo-olefin

copolymer (COC), which combines good optical

properties with biocompatibility and good

chemical resistance to most acids and bases.

A set of connectors is available which allows to

connect standard tubes or typical laboratory tools

such as pipettes or syringes to the fluid ports of

the SMS0104.

snake mixer slide

Technical specifications

max. inlet pressure* min. 6000 hPa ( 87 psi)

operating temperature 10° - 50° C

media temperature 10° - 50° C

storage temperature 10° - 50° C®material COC (Topas )

*at 20°C

~

Dimensionschannel no.* length** width/depth** internal volume* max. particle size

1 21 mm 100 µm 0.2 µl 5 µm

2 121 mm 640 µm 49.6 µl 5 µm

3 66 mm 320 µm 6.8 µl 5 µm

4 50 mm 320 µm 5.1 µl 5 µm

5 16 mm 320 µm 1.6 µl 5 µm

* cf. page 2, top figure

** between confluence and outlet

18

.0

25

.5

4.5

75.5

18.0

4.3

0.2

2.5

1.5

Main features

several mixers on one chip

made of transparent plastic

simple channel geometry (resulting in less fouling trouble)

slide format

standard components

SMS0104

TPS1304

twin pump slide

standard components

fluid splitter slideSCS0105

The SCS0105 fluid splitter slide features one 1-to-

8 and two 1-to-4 microfluidic manifolds. It allows

to divide a sample into up to eight equal streams

for parallel processing.

The slide is made of cyclo-olefin copolymer

(COC), which combines good optical properties

with biocompatibility and good chemical

resistance to most acids and bases.

A set of connectors is available which allows to

connect standard tubes or typical laboratory

tools such as pipettes or syringes to the fluid

ports of the SCS0105.


max. inlet pressure* min. 6000 hPa ( 87 psi)


media temperature 10° - 50° C

storage temperature 10° - 50° C®material COC (Topas )

*at 20°C

~

Main features

several splitters on one chip

made of transparent plastic

renders equal subsamples

slide format

Dimensionschannel no.* length width/depth outlets max. particle size

1 28.9 mm 300 µm 4 5 µm

2 28.9 mm 300 µm 4 5 µm

3 31.7 mm 300 µm 8 5 µm* cf. page 2, top figure

TPS1304

twin pump slide

additional components

cuvette chamber slideCCS0109

Main features

5 viewing chambers

Completed with adhesive to attach cover slips

(Cover slips not included)

Made of transparent plastic

Slide format


operating temperature 10° - 50° C®material COC (Topas )

Slide footprint 25.5 x 75.5 mmStorage: Store at room temperature (21ºC, 50%rel. humidity) out of direct sunlight

The cuvette slide provides a visualization

platform for biological samples that is consistent

with the slides existing in the thinXXS

Construction Kit. The slide consists of 5 viewing

chambers which are connected to separate

fluidic ports via inlet and outlet channels.

Standard glass cover slides (rectangular – 24x60

mm or circular – 10mm dia.) may be used to seal

the chambers and would allow the user to

perform tests with custom treated glass slides or

microarrays. The slide is equipped with an

adhesive to attach the glass cover slips (cover

slips are not included).

The viewing chamber is cylindrical with a

diameter of approximately 3mm and a height of

1,3mm. The corresponding sample volume in the

chamber is 10µl. A rectangular pre-chamber may

be utilized to trap small air bubbles.

top side bottom side

TPS1304

twin pump slide


linear channel slideLCS0109

The LCS0109 features 5 straight channels of

different cross sectional areas.

The slide is made from cyclo-olefin copolymer

COC, which combines excellent optical properties

with biocompatibility and good chemical

resistance to most acids and bases.

The channels are covered with a thin COC film.


max. inlet pressure* min. 6000 hPa (~87psi)


media temperature 10° - 50° C®material COC (Topas )

Slide footprint 25.5 x 75.5 mm*at 20°C

Channel dimensions (width* x depth)

A 500 µm x 300 µm

B 200 µm x 200 µm

C 100 µm x 100 µm

D 50 µm x 50 µm

E 100µm x 50 µm

F 200µm x 50 µm

G 300µm x 50 µm

* at max. width. The slide walls are tapered (~7°)

Main features

8 straight channels

Various geometries


Slide format

TPS1304

twin pump slide


filter slideFs0409

Main features

5 filter chambers

Completed with filter membranes


Slide format

The filter slide provides platform for sample

preconditioning. The slide consists of 5 filter

chambers which are equipped filter membranes.

No adhesives are used to mount the membranes.

Each chamber features two inlets, allowing for

cross-flow filtration.

The slide is made from cyclo-olefin copolymer

COC, which combines excellent optical

properties with biocompatibility and good

chemical resistance to most acids and bases.


The filter is not exchangeable.


max. inlet pressure* min. 6000 hPa (~87psi)


media temperature 10° - 50° C®material COC (Topas )

filter type Whatman Mf1*at 20°C

Dimensions

Slide footprint 25.5 x 75.5 mm

Filter diameter 7 mm

Filter surface area approx. 28 mm²

TPS1304

twin pump slidex-slide

The chip features a simple cross architecture which might be used e.g. for capillary electrophoresis. Wider channels connect the cross geometry with the inlet/outlet ports.

The slide is made from cyclo-olefin copolymer COC, which combines excellent optical properties with biocompatibility and good chemical resistance to most acids and bases.



Max. inlet pressure (at 20°C) 6000hPa

Operating temperature 10°C – 50°C

Media temperature 10°C – 50°C

Storage temperature 10°C – 50°C

Slide Material COC (Topas)

Main features

Simple cross


Slide format

Dimensions

Channel length * width** depth

A 5 mm 50 µm 25 µm

B 5 mm 50 µm 25 µm

C 5 mm 50 µm 25 µm

D 35 mm 50 µm 25 µm

* Between cross intersection wider region of channel

** Measured at maximum width. The side walls are tapered (6°)


TPS1304

twin pump slideH-slide

The chip features a simple H-shaped architecture which might be used e.g. for laminar flow experiments.

The slide is made from cyclo-olefin copolymer COC, which combines excellent optical properties with biocompatibility and good chemical resistance to most acids and bases.








Main features

3 different H-structures


Slide format

Dimensions

Channel length * width** depth

A 18 mm 100 µm 50 µm

27 mm 200 µm 50 µm

13,5 mm 200 µm 50 µm

9 mm 200 µm 50 µm

B

C

D


B AC D

top view bottom view

TPS1304

twin pump slide


blank kit slide







Main features

Blank surface


Slide format

Design your own microfluidic chip

Beside the standard slides for pumping, mixing or splitting the fluid thinXXS offers a blank kit slide where you can design your own fluidics. The geometry of your fluidic layout can be incorporated by milling or laser ablation.

Regarding sealing of the milled parts can we offer our support.

The advantage is that very quickly different fluidic layouts can be tested and optimized before a mould insert is produced.

Outer dimensions

The footprint of the molded parts is based on the microfluidic construction kit. Standard slides have a footprint of 25.5mm x 75.5 mm, which is equivalent to the size of a standard microscopy slide. 28 fluidic access ports are arranged in two lines at the long sides of the slide. Custom channels may be designed in the back of the slide.

The necessary access ports can be drilled into the center of the fluidic ports.The fluidic ports have a pitch of 4.5mm. This is in accordance with the well spacing of a 384 well microplate.

An area of about 62 mm x 15 mm on the opposite side of the fluidic ports is available for individually designed microfluidic structures.

design area

TPS1304

twin pump slide

components

accessories

Main features

easy and flexible handling

combination of up to four slides per platform

tight, but non-permanent connections

connectors and plugs available in COC and PP

Micro connectors and plugs are made of either

COC or PP; they allow to link the fluid ports on

top of the slides with those of adjacent slides

(slide connectors, SLC0405), with typical

laboratory equipment such as syringes (micro

luer connector, MLC0105) and pipettes (micro

pipette connector, MPC0105), or with standard

tubes (micro fluid connector, MFC0204). Micro

plugs (MPG0105), in turn, allow to block selected

fluid ports.

micro plug

slide connectors (“fluid bridges“) lever(used to disconnect slide connectors)

micro pipette connector

type 2x2 type 1x2 type 4x2

micro fluid connector* micro luer connector

*Recommended tubing

type: Tygon Silicone tube

inner diameter: 1.6 mm 1.0 mm

outer diameter: 3.2mm 3.0 mm

material: PVC silicone

thinXXS Microtechnology AG

Amerikastrasse 21D-66482 Zweibruecken, GermanyTel +49-6332-8002-0Fax +49-6332-8002-22E-Mail [email protected] www.thinxxs.de

microfluidic construction kit thinxxs 30mar2011 · fields of microfluidics, - to test fluidic...

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