microscope components and diffractionjokr7175/docs/lesson 6.pdf · microscope components and...
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![Page 1: Microscope components and diffractionjokr7175/docs/Lesson 6.pdf · Microscope components and diffraction • Last class • Imaging • Absorption • Reflection • This class •](https://reader034.vdocument.in/reader034/viewer/2022050512/5f9ccaf77ad96b5682329062/html5/thumbnails/1.jpg)
Microscope components and diffraction
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• Last class• Imaging• Absorption• Reflection
• This class• Components of a microscope• Diffraction
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Anti-reflection coatings
Even at normal incidence, glass reflects ~4% of light
Apply coating with n in between air and glass
One coat of proper index takes reflection 4% -> 2%Set thickness = λ/4
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Real world anti-reflective coatings
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Microscope components
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Parts of a microscope
Light source
Collector Condenser
Objective
Eyepiece
Image
Camera
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Objectives are the most expensive, therefore most important component
Primary image forming opticSet resolution limits downstreamRequired to be small
Metrics for objectives:- Numerical Aperture- Magnification- Field number- Immersion medium- Aberration correction- Working distance- Depth of focus- Transmission
All of these things will contribute to image quality
Objective CFI S Fluor20Xremove
Image
Type Super Fluor
Primary Technique Brightfield
Price $$$$
Immersion Air
Magnification 20X
Numerical Aperture 0.75
Working Distance 1
Cover Glass Thickness 0.17
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Condensers
Concentrate light into a cone that can be adjusted with an iris
Brighter, higher resolution Dimmer, higher contrast
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Eye pieces
• Typically come in 10-20x• Don’t believe that things can
get to 1200x magnification, high mag, just blurry
Eye pieces look at an image formed in the microscope
More money gets you slightly better eyepieces
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Cameras – two flavors
• CCD vs CMOS
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Inverted vs upright
Trans vs epi illumination
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Diffraction – Yet another property of waves
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Diffraction
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Single slit diffraction
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Multi-slit diffraction
Light needs to recombine to form image
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Physical basis for diffraction• Hyugen’s principle• A plane wave is made
up of interfering spherical waves
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On to Matlab…