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From: Gsalaj7-at-aol.com (by way of Ask-A-Microscopist) Date: Sun, 1 Aug 2004 17:36:03 -0400 Subject: [Microscopy] AskAMicroscopist: SEM of eye cyst from a swan

Contents Retrieved from Microscopy Listserver Archives http://www.microscopy.com/MicroscopyListserver/MicroscopyArchives.html

Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Gsalaj7-at-aol.com) from http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Sunday, August 1, 2004 at 14:15:07---------------------------------------------------------------------------

Email: Gsalaj7-at-aol.comName: Sally Thomas Graziano

Organization: Orlando Science Center

Education: Undergraduate College

Location: Orlando Florida

Question: In my work on the SEM here at OSC. Along with our educational programs we have an ongoing research project. In that regard I have a very small eye cyst from a swan which I need to look at. As you can see most of my specimens are larger biological specimens. How should I mount it on the SEM stub. I use carbon tape for most things but am afraid the cyst will get lost in one of the bubbles of the tape.Also how long to coat. Any sugestions would be most helpful. Sally Graziano

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From: David Vowles : djv23-at-msm.cam.ac.uk Date: Mon, 2 Aug 2004 09:30:25 +0100 (BST) Subject: [Microscopy] Re: viaWWW: Export EDX spectrum


Juan,

You wrote:

} I took some EDX spectrum by using EDAX Phoenix 1998 Revision 3.1, but I } can not export the spectrum into a data file. Does anybody know the method? } Any suggestion would be highly appreciated.} EDAX have a free spectrum viewer that can be downloaded from: http://www.edax.com/support/EDS_Spectrum_Viewer.html

You can cut and paste the channel data from this programmme.

Alternatively, I have written an application which will display EDAX .spc files - as well as Noran, Link/Oxford and EMSA file formats - which allows export of data as well. Let me know if you want a copy.

David VowlesElectron Microscope UnitDept of Materials Science and MetallurgyUniversity of CambridgePembroke St CambridgeUK CB2 3QZTel: +44 (0)1223 334325Fax: +44 (0)1223 334567Email: djv23-at-cam.ac.uk

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From: Giles, Bill : William.Giles-at-TIMET.com Date: Mon, 2 Aug 2004 11:38:58 -0600 Subject: [Microscopy] Metallographic Sample Storage Containers


Hey Listers,

We are looking for a source of individual metallographic sample storagecontainers. Our mounts are typically 1" diameter and 0.5-1" tall.

Anyone have a source for these?

William GilesSenior Electron MicroscopistMetallography Lab CoordinatorP.O. Box 2128Henderson, Nevada 89009(702) 566-4436(702) 564-9038 (Fax)William.Giles-at-timet.com

*

From: Ritchie Sims : r.sims-at-auckland.ac.nz Date: Tue, 03 Aug 2004 06:28:05 +1200 Subject: [Microscopy] Asbestos by SEM?


Hi

Can someone point me in the direction of online information regarding techniques for the identification of asbestos by SEM and by other techniques?

thanks

rtch

--Ritchie Sims Ph DPhone : 64 9 3737599 ext 87713Microanalyst Fax : 64 9 3737435Department of Geology email : r.sims-at-auckland.ac.nzThe University of Auckland Private Bag 92019AucklandNew Zealand

From: White, Woody N. : nwwhite-at-bwxt.com Date: Mon, 2 Aug 2004 15:07:59 -0500 Subject: [Microscopy] Metallographic Sample Storage Containers


Let us know if you find something that is suitable and *inexpensive*.Microscopy supply vendors sell plastic boxes, but at the rate I go throughsamples, I would go broke and run out of storage room as well.

No inexpensive vials, jars, etc. that I have found protect the preparedsurface well - no mounting method. The best compromise is to use a snuglyfitting "Cap Plug" (poly closure used to close pipe ends, other holes) andpartially insert the mount into it.

If nothing else fits, I stuff the large mounts into a locking closureplastic bag. It is likely that if fragile or coated, this will harm thespecimen, however.

A great solution (not my idea) for 1/2" (12mm) pin-mounts is an "Opticlear"25 dram glass vial with a closed bottom poly cap. Punch or cut a snug holein the bottom (inside) of the cap, shove the stub pin in and place invial...

Regards,Woody

Woody White BWXT Services:http://www.bwxt.com/bwxt.htmlMy Site:http://woody.white.home.att.net

-----Original Message-----} From: Giles, Bill [mailto:William.Giles-at-TIMET.com] Sent: Monday, August 02, 2004 1:39 PMTo: 'Microscopy-at-microscopy.com'

Hey Listers,




*

From: David Henriks : henriks-at-southbaytech.com Date: Mon, 02 Aug 2004 14:23:34 -0700 Subject: [Microscopy] Re: Metallographic Sample Storage Containers


Bill:

We have some containers that are ideal for this application. You can see an image of them on our website at www.southbaytech.com. Do a keyword search for "containers". You will also find them listed with our extensive range of metallographic consumables under the "consumables" button. I will contact you off-line with pricing.

Best regards-

David

Giles, Bill wrote:

} ------------------------------------------------------------------------------} The Microscopy ListServer -- Sponsor: The Microscopy Society of America

-- David HenriksVice President

South Bay Technology, Inc.1120 Via CallejonSan Clemente, CA 92673 USA

TEL: +1-949-492-2600Toll-free in the USA: +1-800-728-2233FAX: +1-949-492-1499

email: henriks-at-southbaytech.com

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Please visit us online at www.southbaytech.com.

The information contained in this message and any attachments is privileged and confidential. This message is intended for the individual or entity addressed. If you are not the intended recipient, please do not read, copy or disclose this communication. Notify the sender of the mistake by calling +1-949-492-2600 and delete this message from your system.

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From: Gregory Mulhollan : gmulhollan-at-austin.rr.com Date: Mon, 2 Aug 2004 16:38:51 -0500 Subject: [Microscopy] Seeking manual for TN6500


Hello Microscopy folks,I am trying to resurrect an old spectroscopy system that uses a Tracor Northern TN-6500 box for controlling a linear diode readout. Anyone out there with a manual for the TN-6500? I am happy to pay photocopy charges or do anything (reasonable!) to get my hands on one. Thanks again.Greg Mulhollan-----------------------------------Gregory Mulhollan, Ph.D.Saxet Surface Science1001 S. Sunset Canyon DriveDripping Springs, TX 78620(512)858-2841 office(512)694-4879 cellmulhollan-at-saxetsurfacescience.comwww.saxetsurfacescience.com

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From: Garber, Charles A. : cgarber-at-2spi.com Date: Tue, 03 Aug 2004 02:10:03 -0500 Subject: [Microscopy] Storage containers for 1 metallographic mounts


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

William Giles wrote:==========================================================================================We are looking for a source of individual metallographic sample storagecontainers. Our mounts are typically 1" diameter and 0.5-1" tall.

Anyone have a source for these?==========================================================================================SPI Supplies has manufactured for some years our special mount storage boxfor 1" mounts, see URLhttp://www.2spi.com/cataelog/boxes/speci_box.shtml

This would be our SPI #02020-AB and each one holds eight mounts. There areinserts for desiccating capsules. And once the samples are loaded into thebox, and the lid closed, some ordinary Scotch Tape is put around the lip ofthe closed box to seal out air or anything else that might seep in anddamage samples.

Disclaimer: SPI Supplies is the manufacturer of this special kind ofstorage box.

Chuck

===================================================Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400President 1-(800)-2424-SPISPI SUPPLIES FAX: 1-(610)-436-5755PO BOX 656 e-mail: cgarber-at-2spi.comWest Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com

Look for us! ############################ WWW: http://www.2spi.com ############################==================================================

From: Giles, Bill : William.Giles-at-TIMET.com Date: Tue, 3 Aug 2004 07:51:12 -0600 Subject: [Microscopy] Re: Met Sample containers


Thanks for all the responses; I'll filter thru all the suggestions.


*

From: Szewczyk, Steven (Cont, ARL/WMRD) : sszewczyk-at-arl.army.mil Date: Tue, 3 Aug 2004 10:44:38 -0400 Subject: [Microscopy] Metallographic Sample Storage Containers


We use the "Cap Plugs" Woody has suggested and I find they work well ifyou're looking for inexpensive (a few cents per piece) individual samplestorage.

The caps come in a variety of shapes and sizes, and I'm sure there aremany sources for such items. The vendor we ordered from has a good pagewith drawings and dimensions for each:http://www.niagaracapsandplugs.com/nonthreaded_caps.htm

Regards,

Steven T SzewczykMaterials Science ContractorUS Army Research LabAberdeen Proving Ground, MD

-----Original Message-----} From: White, Woody N. [mailto:nwwhite-at-bwxt.com] Sent: Monday, August 02, 2004 4:08 PMTo: 'Giles, Bill'; 'Microscopy-at-microscopy.com'

Let us know if you find something that is suitable and *inexpensive*.Microscopy supply vendors sell plastic boxes, but at the rate I gothrough samples, I would go broke and run out of storage room as well.

No inexpensive vials, jars, etc. that I have found protect the preparedsurface well - no mounting method. The best compromise is to use asnugly fitting "Cap Plug" (poly closure used to close pipe ends, otherholes) and partially insert the mount into it.


A great solution (not my idea) for 1/2" (12mm) pin-mounts is an"Opticlear" 25 dram glass vial with a closed bottom poly cap. Punch orcut a snug hole in the bottom (inside) of the cap, shove the stub pin inand place in vial...

Regards,Woody


-----Original Message-----} From: Giles, Bill [mailto:William.Giles-at-TIMET.com]Sent: Monday, August 02, 2004 1:39 PMTo: 'Microscopy-at-microscopy.com'

Hey Listers,




*

From: George Langford, Sc.D. : amenex-at-amenex.com Date: Tue, 3 Aug 2004 11:40:28 -0400 (EDT) Subject: [Microscopy] Re: Commercial SEM labs in the Cleveland, OH area ?


Hello Microscopists !

Quite some time ago, in anticipation of a congenial meeting of interestedparties at a common-ground investigation of several broken steel bolts inthe Cleveland, Ohio, area, I asked about suitable SEM labs in the area.I received an excellent response from The List, which indicates that it isthe primary means of rapid and effective communication in the micrsocopycommunity. Alas, there was a slower response from the above-referencedinterested parties, and so no agreement about protocol or venue wasreached until we had all retired to our own offices. Later on, we wound upgoing back to the place selected originally by one of the parties way backat the beginning of the project, where 1000X is about the limit of usefulmagnification. I am sure any one of you could have done better.

Thanks to all who reponded.

Best regards,George Langford, Sc.D.Principal ConsultantAmenex Associates, Inc.amenex-at-amenex.comhttp://www.amenex.com/

From: elliott : elliott-at-uark.edu Date: Tue, 03 Aug 2004 14:39:33 -0500 Subject: [Microscopy] Wehnelt cleaning


Mr. Chapman,When you say "stock ammonia solution", are you talking about standard Ammonia(NH3)?

From: cnorton-at-wis-inc.net (by way of MicroscopyListserver) Date: Tue, 3 Aug 2004 19:52:30 -0400 Subject: [Microscopy] viaWWW: searching for service engineers


Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (cnorton-at-wis-inc.net) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, August 3, 2004 at 15:36:41---------------------------------------------------------------------------

Email: cnorton-at-wis-inc.netName: chris norton

Organization: Wafer Inspection Services, Inc.

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: We are searching for service engineers with KLA-Tencor service and applications experience on all models.Prometrix UV1250/1280; RS 75; 6420; AIT; Starlight; 8100 CD SEMS; etc.

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From: garyeaston-at-scannerscorp.com (by way of MicroscopyListserver) Date: Tue, 3 Aug 2004 19:51:53 -0400 Subject: [Microscopy] viaWWW: Cambridge/Leica/LEO S360 SEM


Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (garyeaston-at-scannerscorp.com) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, August 3, 2004 at 13:34:53---------------------------------------------------------------------------

Email: garyeaston-at-scannerscorp.comName: Gary M. Easton

Organization: Scanners Corporation

Title-Subject: [Microscopy] [Filtered] Cambridge/Leica/LEO S360 SEM

Question: I just picked up a used Cambridge S360 that gives me an error code 307(defective LAB6 switch). Does anyone out there that has a complete list of the software error codes and their explanation? The operator's manual offers no help. Also, if anyone has the setup software (mag cal,etc) for this series SEM and is willing to part with a copy, it would be greatly appreciated. Please reply offline to garyeaston-at-scannerscorp.com. Thanks in advance.

Gary M. Easton

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From: David Elliott : David.Elliott-at-yale.edu Date: Wed, 4 Aug 2004 08:09:17 -0700 Subject: [Microscopy] Leica ultramicrotome


Hi allI am looking at Leica ultra-microtomes. The new version (the UC6b) has two controllers, the key pad or the touch screen. I would appreciate any input or comments on these. I am especially interested in reasons to get one or the other.ThanxDavid

_____________________

David Elliott Ph.D.Research Assistant ProfessorDepartment of Cell Biology and AnatomyPO Box 245004Tucson, AZ 85724

Voice: 520-626-7870Fax: 520-626-2097

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From: Ann St. Amand : astamand-at-phycotech.com Date: Wed, 04 Aug 2004 11:57:12 -0400 Subject: [Microscopy] Need to borrow or rent 11 LM Phase scopes for a workshop in


Hi all, I am co-chair of a workshop being given on algae identification at the North American Lakes Management Society International Symposium in Victoria Canada. The workshop is being offered November 2, 2004. Normally, its not a problem to get the scopes, but I can't find a source in Canada. I need 10 teaching scopes with Phase and 400 objectives and 1 BX60 with Phase at 200 and 400 with a trinoc head and a digital camera. Can anyone help? I'm located in Michigan, United States. Thanks much, Ann.

Ann St. Amand, Ph.D.PresidentPhycoTech, Inc.620 Broad St., Suite 100St. Joseph, Michigan 49085 USA

269-983-3654 (voice)269-983-3653 (fax)mailto:astamand-at-phycotech.comwww.phycotech.com

Specializing in Aquatic Sample Analysis and Microscope Accessories

Director, Region V, North American Lake Management Society, www.nalms.org

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From: Sergey Ryazantsev : sryazant-at-ucla.edu Date: Wed, 04 Aug 2004 11:21:00 -0700 Subject: [Microscopy] Re: Leica ultramicrotome


Hi DavidKeypad does not have all features, touch screen has. It's quite confusing. For instance if you go with touch pad, you need to buy $5K interface (whichever it called) for their new cryo-attachment and so on. As far as I understand, touch-screen is sort of "standard" and they "invented" key-pad to make cheaper version of the ultratome. You need to check the compatibility issue, because UC6 is not compatible with previous generation stuff, like UCT, FSC etc. Good luck with shopping. Sergey

At 08:09 AM 8/4/2004, you wrote:


_____________________________________

Sergey Ryazantsev Ph. D.Electron MicroscopyUCLA School of MedicineDepartment of Biological Chemistry10833 Le Conte Ave, Room 33-089Los Angeles, CA 90095

Phone: (310) 825-1144 (office) (310) 206-1029 (Lab)FAX (departmental): (310) 206-5272mailto:sryazant-at-ucla.edu

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From: matthew.salanga-at-childrens.harvard.edu (by way of Ask-A-Microscopist) Date: Wed, 4 Aug 2004 22:04:22 -0400 Subject: [Microscopy] AskAMicroscopist: CFPYFP FRET control slides


Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (matthew.salanga-at-childrens.harvard.edu) from http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html#form on Wednesday, August 4, 2004 at 15:15:22---------------------------------------------------------------------------

Email: matthew.salanga-at-childrens.harvard.eduName: Matthew Salanga

Organization: Children's Hospital Boston

Education: Graduate College

Location: Boston, MA

Question: Greetings!

Does anybody know where I can obtain CFP-YFP FRET control slides. Or transfected a stable FRET positive cell line. Ideally I am looking for cells grown on coverslips which have tagged CFP and YFP proteins that are known to elicit a FRET response.

Thanks!

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From: sspence-at-flemingc.on.ca (by way of Ask-A-Microscopist) Date: Wed, 4 Aug 2004 22:05:36 -0400 Subject: [Microscopy] AskAMicroscopist: Grade School Teacher Needs Pictures of Cells


Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (sspence-at-flemingc.on.ca) from http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Wednesday, August 4, 2004 at 19:41:48---------------------------------------------------------------------------

Email: sspence-at-flemingc.on.caName: Susan Spence

Organization: Tottenham Public School

Education: 6-8th Grade Middle School

Location: Tottenham, Ontario Canada

Question: I have a very ruly group of grade 8's, very few microscopes, and even fewer quality slides from which to view organelles and unicellular organisms. DO you have any suggestions as to where I may find good images, either from a light microscope or an electron microscope? I want to show them what cells really look like through a microscope without actually having them to touch one. Thanks for your suggestions!

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From: James Chalcroft : jchalcro-at-neuro.mpg.de Date: Thu, 5 Aug 2004 10:08:02 +0200 Subject: [Microscopy] Leica ultramicrotome


The Leica ultra-microtome ....?!This brings back memories of the original one, based on the design byHumberto Fernandez-Moran about 50 years ago. Anyone still remember it?Its design was absolutely unique in that the specimen chuck was mountedin a motor-driven massive cylinder which performed complete rotations inthe horizontal plane over two pairs of huge flat sapphire bearingsplaced in a "V" orientation. I imagine that it must have been a "cow ofa thing" to set up! It was possibly developed to utilize the newlyinvented diamond knives (also invented by F-M, whose genius contributedso much to new developments in the early days of TEM). I only ever sawadvertising brochures of the ultra-microtome, so would be interested tolearn of users' experiences from those times and perhaps also somethingabout the fate of these now-historic instruments.

-----Original Message-----} From: David Elliott [mailto:David.Elliott-at-yale.edu] Sent: Wednesday, August 04, 2004 5:09 PMTo: Microscopy ListServer


_____________________


Voice: 520-626-7870Fax: 520-626-2097

From: todd hamm : ripbnowell-at-hotmail.com Date: Thu, 05 Aug 2004 08:56:02 -0500 Subject: [Microscopy] Ultramicrotome problems


I do remember setting up something like that. Except my recollection is that it was designed by Sjostrand. The cylindrical chuck unit had a groove around it so that you would mount the motor on the wall, and run a long V-belt from the motor to the microtome. This was to minimize vibration. The other feature that I remember is that the cutting speed was quite fast, not at all like what we use today.

Joel

I am using a Reichart-Jung MT 6000 ultramicrotome for standard EM sectioning. After the specimen goes through the cutting range there is a beep and the microtome electronics will freeze up, I have tried reseting the knife and specimen advancements but the problem still persists, does anyone have a recommendation?

Thanks,

Todd HammResearch TechnicianOklahoma Medical Research Foundationtmhamm09-at-yahoo.com

_________________________________________________________________Express yourself instantly with MSN Messenger! Download today - it's FREE! http://messenger.msn.click-url.com/go/onm00200471ave/direct/01/

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From: Timo Junker : timojunker-at-holografie.com Date: Thu, 05 Aug 2004 16:54:08 +0200 Subject: [Microscopy] found this, maybe helps others old TEMs interfaced with digital


On my search for the possibility to get high tension and magnificationvalues out of older Philips and Zeiss TEMs I got no answer from the groupand finally found after an extensive search of the web the followinginterface-cards for these TEMs:

For Philips EM 400, 410, 420: http://www.stefan-diller.com/rem_tem3_en.htmFor Zeiss TEMs EM 10 A, B and C: http://www.stefan-diller.com/rem_tem4_en.htm

I hope this is useful for somebody having the same problems with wrongmag-values and doing new reference images after changing the HT-value on anold TEM...

-- Timo Junker HolografieLindenstr. 1097297 WaldbttelbrunnTel: ++49 (0)931 4520655Fax: ++49 (0)931 4520657www.holografie.com

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From: Joel Sheffield : jbs-at-temple.edu Date: Thu, 05 Aug 2004 12:04:49 -0400 Subject: [Microscopy] RE: RE: Leica ultramicrotome History


Dear Joel,

Thanks for your comments. The ultra-microtome as such was of course invented by Fritiof Sjostrand and I agree that his first models had a remote motor, just as you describe. I did not realize that his design also originally had 360 rotation of the specimen chuck holder. As far as I know his design was developed in conjunction with LKB-Produkter, Bromma Sweden whereas the slightly later Fernandez-Moran instrument was developed with Ernst Leitz, Wetzlar Germany. I seem to recollect that an example of the F-M instrument found its way to Dunedin, New Zealand, but there must have been others too.Perhaps others might be interested in taking the story further from here....Cheers,

Jim

-----Original Message-----} From: Joel Sheffield [mailto:jbs-at-temple.edu] Sent: Thursday, August 05, 2004 3:05 PMTo: James Chalcroft; Microscopy ListServer

Dear Jim,

I saw the Sjostrand microtome when I taught a course at Rutgers, Camden, in the 1970's. We actually got it set up and running. They also had a (barely) working RCA EMU-2 scope. I doubt if they have kept either.

The other thread in this history, of course, was the system developed by Keith Porter. In the original models of what eventually became the Porter-Blum microtome, the block followed the famous parallelogram path, but advance was thermal. We had a goosneck lab light mounted over the rod that held the specimen, and you sat there flashing the light with each pass of the block. If you were good, you could get beautiful ribbons of silver --but woe betide anyone who walked past you while you were sectioning!

Later on, Keith developed the offset gimble mechanical advance that led to the Sorval series of MT microtomes.

The other interesting approach to microtomy was that of Huxley, who used flexible springs for all of the hinge points, to replace bearings and reduce vibration. This machine was originally made by Cambridge, and later distributed by LKB.

} Dear Joel,} } Thanks for your comments. The ultra-microtome as such was of course} invented by Fritiof Sjostrand and I agree that his first models had a} remote motor, just as you describe. I did not realize that his design} also originally had 360 rotation of the specimen chuck holder. As far} as I know his design was developed in conjunction with LKB-Produkter,} Bromma Sweden whereas the slightly later Fernandez-Moran instrument} was developed with Ernst Leitz, Wetzlar Germany. I seem to recollect} that an example of the F-M instrument found its way to Dunedin, New} Zealand, but there must have been others too. Perhaps others might be} interested in taking the story further from here.... Cheers,} } Jim} } -----Original Message-----} From: Joel Sheffield [mailto:jbs-at-temple.edu] } Sent: Thursday, August 05, 2004 3:05 PM} To: James Chalcroft; Microscopy ListServer} Subject: [Microscopy] Re: RE: Leica ultra-microtome (History)} } I do remember setting up something like that. Except my recollection} is that it was designed by Sjostrand. The cylindrical chuck unit had} a groove around it so that you would mount the motor on the wall, and} run a long V-belt from the motor to the microtome. This was to} minimize vibration. The other feature that I remember is that the} cutting speed was quite fast, not at all like what we use today.} } Joel} } } Subject: [Microscopy] RE: Leica ultra-microtome (History)} Date sent: Thu, 5 Aug 2004 10:08:02 +0200 From: } "James Chalcroft" {jchalcro-at-neuro.mpg.de} To: "David} Elliott" {David.Elliott-at-yale.edu} Copies to: "Microscopy} ListServer" {Microscopy-at-MSA.Microscopy.Com} } } } } } } } --------------------------------------------------------------------} } -- -------- The Microscopy ListServer -- Sponsor: The Microscopy} } Society of America To Subscribe/Unsubscribe --} } http://www.msa.microscopy.com/MicroscopyListserver On-Line Help} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html} } --------------------------------------------------------------------} } -- ---------} } } } The Leica ultra-microtome ....?!} } This brings back memories of the original one, based on the design} } by Humberto Fernandez-Moran about 50 years ago. Anyone still} } remember it? Its design was absolutely unique in that the specimen} } chuck was mounted in a motor-driven massive cylinder which performed} } complete rotations in the horizontal plane over two pairs of huge} } flat sapphire bearings placed in a "V" orientation. I imagine that} } it must have been a "cow of a thing" to set up! It was possibly} } developed to utilize the newly invented diamond knives (also} } invented by F-M, whose genius contributed so much to new} } developments in the early days of TEM). I only ever saw advertising} } brochures of the ultra-microtome, so would be interested to learn of} } users' experiences from those times and perhaps also something about} } the fate of these now-historic instruments.} } } } -----Original Message-----} } } From: David Elliott [mailto:David.Elliott-at-yale.edu] } } Sent: Wednesday, August 04, 2004 5:09 PM} } To: Microscopy ListServer} } Subject: [Microscopy] Leica ultra-microtome } } } } } } } } --------------------------------------------------------------------} } -- -- ------ The Microscopy ListServer -- Sponsor: The Microscopy} } Society of America To Subscribe/Unsubscribe --} } http://www.msa.microscopy.com/MicroscopyListserver On-Line Help} } http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html} } --------------------------------------------------------------------} } -- -- -------} } } } Hi all} } I am looking at Leica ultra-microtomes. The new version (the UC6b)} } has two controllers, the key pad or the touch screen. I would} } appreciate any input or comments on these. I am especially} } interested in reasons to get one or the other. Thanx David} } } } } } _____________________} } } } David Elliott Ph.D.} } Research Assistant Professor} } Department of Cell Biology and Anatomy} } PO Box 245004} } Tucson, AZ 85724} } } } Voice: 520-626-7870} } Fax: 520-626-2097} } } } } } } } } } Joel B. Sheffield, Ph.D} Department of Biology} Temple University} Philadelphia, PA 19122} Voice: 215 204 8839} e-mail: jbs-at-temple.edu} }

Joel B. Sheffield, Ph.D.Biology Department, Temple University1900 North 12th StreetPhiladelphia, PA 19122jbs-at-temple.edu (215) 204 8839, fax (215) 204 0486http://astro.temple.edu/~jbs

From: Sergey Ryazantsev : sryazant-at-ucla.edu Date: Thu, 05 Aug 2004 11:07:28 -0700 Subject: [Microscopy] Re: RE: Leica ultramicrotome History


Leica ultratomes originated from Ultracut series by Reichert-Jung. Am I correct? It seems to me, Leica did not have their own ultratome development (would be nice to know details if they did). They just bought Reichert-Jung and LKB both. They killed SuperNova (LKB) and continued Ultracut. Sergey

At 01:08 AM 8/5/2004, you wrote:


_____________________________________

Sergey Ryazantsev Ph. D.Electron MicroscopyUCLA School of MedicineDepartment of Biological Chemistry10833 Le Conte Ave, Room 33-089Los Angeles, CA 90095

Phone: (310) 825-1144 (office) (310) 206-1029 (Lab)FAX (departmental): (310) 206-5272mailto:sryazant-at-ucla.edu

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From: James Chalcroft : jchalcro-at-neuro.mpg.de Date: Fri, 6 Aug 2004 10:45:59 +0200 Subject: [Microscopy] more Leica ultramicrotome history


Aha! Joel, Sergey and anyone else interested in historical matters,

I got motivated after reading your interesting comments and actually located here an old Leica brochure (53-12b, in German) dated June 1964, which described the original "Leica Ultra-Mikrotom nach Fernndez-Morn". It was equipped with a Rawyler diamond knife "of highest quality" and was apparently designed to cut just about anything. The accompanying TEM images show sectioned cells in Vestopal and Methacrylate also an Al/Ag alloy. The 3 kg steel rotor was belt-driven with 2 motors situated below the desk (one was used for the sectioning phase of rotation and cutting speed could be controlled from 50 down to 3 mm/sec, while the fast second motor took over for the rest of the rotation cycle).The object chuck was at the front of a long diametrically placed cylindrical invar holder (fastened at its back end to the back of the rotor) which had an inbuilt heating element, and the free front end moved by thermal expansion towards the knife for up to 30 mins. After that period the complete holder was removed and allowed to cool down for 15 mins, during which time a second holder could be used for sectioning.Visualization during sectioning was done with a 72x (Leitz Greenough-type?) stereomicroscope and associated fluorescent lamp. A plexiglas hood was supplied to protect the system from air movements and temperature fluctuations during sectioning.Other statistics: Weight (+ table) was 148 kg. Power draw-off was 130 W.The images shown corresponded to typical good quality methacylate sections of the 1950/60s but the instrument never became popular. I imagine that this brochure was one of the last that Leitz issued. It would seem that the main advantage of this instrument design was that complete 360 rotation obviated the need for specimen retraction. Retraction during the upwards stroke is absolutely necessary for any reciprocating system in order to prevent wetting of the knife front or even breakage of the delicate cutting edge when the specimen moves upwards after cutting because all thermal expansion systems have great inherent inertia and advance cannot be stopped successfully for such short time periods.One possible disadvantage of the system is the need for rotational bearings.I do remember vaguely another old ultra-microtome brochure from Leica which had a graph showing the excellent reproducibility of specimen position during cutting from one cycle to the next. This may be so (since German precision workmanship in those years was unbeatable) but the inherent fine-scale bearing rumbling, even if reproducible, would result in a slightly corrugated cut as the specimen would oscillate a little forwards and backwards with respect to the knife during cutting. Perhaps it would need only 10 nm lateral movements in a 100 mm thick section to be noticeable when highest quality ultra-thin sections were examined. Perhaps I am talking somewhat off the top of my head in this case but it would now be interesting to hear from others the real reason(s) for the unpopularity of this long-discontinued instrument - the very FIRST Leica Ultra-Microtome.Best regards & good sectioning to you all ...

Jim

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From: Gerald Bourne : grb-at-ufl.edu Date: Fri, 06 Aug 2004 11:05:07 -0400 Subject: [Microscopy] ion beam coater targets


Hello folks,

I have a Gatan mod. 681 Ion Beam Coater and I'm looking for targets for it. I'm on a limited budget so I'd rather not pay Gatan's prices. Anyone have sources or ideas for Au and Ti targets?

Jerry

-- Gerald BourneMajor Analytical Instrumentation CenterDepartment of Materials Science and EngineeringUniversity of Florida100 Rhines HallP.O. Box 116400Gainesville, FL 32611(352) 392-6985(352) 392-0390 fax

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From: Marie-Claude Blanger : mcbelanger6-at-hotmail.com Date: Fri, 06 Aug 2004 17:04:12 +0000 Subject: [Microscopy] camera, image format


Dear all,

We have a QImaging camera Retiga 1300i 12 bits. The company we deal with for the image acquisition (with a motorized microscope) uses the 16 bit format. So, they transform the 12 bit images to 16 bit images.

We want to use deconvolution on these images. Will the transformation to 16 bits impair image quality?

Thank you, have a good week-end!

Marie-Claude Belanger

_________________________________________________________________Balayez vos courriels entrants et sortants et les pices jointes et contribuez liminer les virus destructeurs susceptibles dy tre intgrs. http://join.msn.com/?pgmarket=fr-ca&page=features/virus Commencez ds maintenant profiter de tous les avantages de MSN Premium et obtenez les deux premiers mois GRATUITS*.

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From: David Henriks : henriks-at-southbaytech.com Date: Fri, 06 Aug 2004 10:17:41 -0700 Subject: [Microscopy] Re: ion beam coater targets


Jerry:

While we do make our own Ion Beam Sputter Deposition and Etching System, we can supply targets for the Gatan system as well. You will find our prices to be *very* reasonable. Please contact me off-line about your specific requirements and I will send you a quote.

Best regards-

David

-- David Henriks







Gerald Bourne wrote:

} } } ------------------------------------------------------------------------------ } } The Microscopy ListServer -- Sponsor: The Microscopy Society of America} To Subscribe/Unsubscribe -- } http://www.msa.microscopy.com/MicroscopyListserver} On-Line Help http://www.msa.microscopy.com/MicroscopyListserver/FAQ.html} ------------------------------------------------------------------------------- } } } Hello folks,} } I have a Gatan mod. 681 Ion Beam Coater and I'm looking for targets } for it. I'm on a limited budget so I'd rather not pay Gatan's } prices. Anyone have sources or ideas for Au and Ti targets?} Jerry}

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From: Gary Gaugler : gary-at-gaugler.com Date: Fri, 06 Aug 2004 12:22:36 -0700 Subject: [Microscopy] Re: camera, image format


Image quality is highest using the 16-bit format.Since the original image is 12-bits, imagequality would suffer (mostly due to loss of dynamicrange) if converted to 8-bits. The conversionfrom 12-bits to 16-bits is typically accomplishedby zero filling the four high order bits. Consequently,the image data is in all eight bits of the low orderbyte and in the four low order bits of the high order byte.

If your deconvolution program can handle 16-bit images,there should be no image degradation. Be advised that,depending on the app itself, your memory requirementsmay be more than you have available. Very often onecan get "Out of Memory" error messages. With modestpixel density and 16-bit TIFF files, you should be OK.

gary g.

At 10:04 AM 8/6/2004, you wrote:

} Dear all,} } We have a QImaging camera Retiga 1300i 12 bits. The company we deal with } for the image acquisition (with a motorized microscope) uses the 16 bit } format. So, they transform the 12 bit images to 16 bit images.} } We want to use deconvolution on these images. Will the transformation to } 16 bits impair image quality?} } Thank you, have a good week-end!} } Marie-Claude Belanger

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From: Alan Nicholls : nicholls-at-uic.edu Date: Fri, 06 Aug 2004 15:34:42 -0500 Subject: [Microscopy] Re: RE: UPS systems and magnetic fields


Hendrik

We also have our 2010F running on a UPS system. I have just done some measurements and while, yes, there is a very large field at the unit (} 500mG rms - off scale for my EMF meter) this decays away rapidly and drops below 1mG rms seven feet from the unit. In our case the column is about 15' from the UPS and we see no effect.

We are still using the same batteries as delivered in 1998. The 50% life time in the case of a power failure has diminished but they still provide sufficient protection for the field emission tip. The battery specifications from the manufacturer states 200 complete full load discharges - we were told a typical life was 4-5 years depending on the number of excursions. Through the manufacturer replacement of all the batteries was quoted at $5K.

One thing to beware of, on multi-phase UPS systems if you are prone to one phase ONLY dying you can fry the UPS control board and you will need a voltage regulator before the UPS unit to protect the UPS.

Regards

Alan

At 11:08 AM 7/23/2004 -0700, Mardinly, John wrote:


Alan W Nicholls, PhDDirector of Research Service Facility (Electron Microscopy)Research Resources Center - East (M/C 337)Room 100 Science and Engineering South BuildingThe University of Illinois at Chicago845 West Taylor StChicago, IL 60607-7058

Tel: 312 996 1227Fax: 312 996 8091Office: Room 110

Web site www.rrc.uic.edu

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From: Richard Edelmann : edelmare-at-MUOhio.edu Date: Mon, 9 Aug 2004 15:54:11 -0400 Subject: [Microscopy] Re: Scanner


Robert:

We have a ArtixScan 2500. (as well as two Agfa Duoscan's). The software interface was a little cumbersome to use, but the image quality was very good.

However, I strongly recommend against Microtek. On the 2500 the lamp stays on all the time (i.e. no standby) - o.k., not a problem just wear on the lamp. The lamp burned out after 8 months - o.k., no problem we replaced many lamps in previsouly years on several scanners - 5 minutes later lamp in hand. . . Can NOT get a replacment lamp. We've been looking every where. Microtek will NOT sell the lamps, they wanted us to send the scanner (67 lbs) to across country and they would sell us a new shipping box. Fine - but 7 additional months later they can't come up with a shipping box, nor can they come up with a price for the lamp replacment and NOW they are telling us we're out of the 12 month warranty.

So here we sit - $3k scanner useless and for a $30 part.

} } I am looking for comment on overall functionallity of the Microtek} ArtixScan 1800f scanner. Reliability, resoultion, speed. Will use this} to scan EM neg. etc.} } Thanks} } Robert J. Kayton, Ph.D.} C.R.O.E.T. L606} Oregon Health & Science Univ.} kayton-at-ohsu.edu} 503-494-2504-Lab} 503-703-3938-Cell} www.ohsu.edu/croet/facilities/emicroscopy} }

Richard E. Edelmann, Ph.D.Electron Microscopy Facility Director350 Pearson HallMiami University, Oxford, OH 45056Ph: 513.529.5712 Fax: 513.529.4243E-mail: edelmare-at-muohio.eduhttp://www.emf.muohio.edu

"RAM disk is NOT an installation procedure."

From: Mark Aindow : m.aindow-at-uconn.edu Date: Fri, 30 Jul 2004 13:03:57 -0400 Subject: [Microscopy] Postdoc Position


University of ConnecticutInstitute of Materials Science

Postdoctoral PositionSPM manipulation of carbon nanotubes and biological systems

A post-doctoral position is available immediately within the Instituteof Materials Science at UConn to work on SPM imaging, manipulation, andlithography of carbon nanotubes and biological systems. The idealcandidate will have experience with AFM measurements in liquid, SPMlithography, and or SPM technique development. Experiments will beconducted in the newly constructed NanoMeasurement labs, featuring twonew AFM systems for in vitro and air measurements with simultaneousconfocal microscopy, as well as a UHV AFM/STM.

This position is a one-year appointment, with funding available forfurther years. To apply, please send a complete resume, together with alist of publications and contact details for 3 references, to Prof.Bryan Huey (bhuey-at-ims.uconn.edu). Screening of applications will beginimmediately, and will continue until the position is filled.Applications are encouraged from under-represented groups, includingminorities, women, and people with disabilities.

Bryan D. HueyThe Institute of Materials ScienceUniversity of Connecticut97 N. Eagleville Road, unit 3136Storrs, CT 06269 USAoffice: (860) 486 3284fax: (860) 486 4745http://www.ims.uconn.edu/bhuey-at-uconn.edu

From: gazzzoman-at-yahoo.com (by way of MicroscopyListserver) Date: Sat, 31 Jul 2004 21:23:50 -0400 Subject: [Microscopy] viaWWW: hybrid AFMoptical microscope


Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (gazzzoman-at-yahoo.com) from http://www.microscopy.org/MicroscopyListserver/MLFormMail.html on Thursday, July 29, 2004 at 09:21:46---------------------------------------------------------------------------

Email: gazzzoman-at-yahoo.comName: daniele gazzola

Organization: Biomed dept. -at-Brown Univ., Providence, RI, USA - Engineering dept. -at-Genova University, Italy

Title-Subject: [Microscopy] [Filtered] hybrid AFM-optical microscope

Question: I am trying to set up a hybrid microscope in order to use AFM (atomic force microscope) along with light microscopy(phase) on unstained biological samples.

The AFM that I am using adds two lenses to the optical path just above the sample. Unfortunately I neither know the specifications of those lenses, nor the characteristics of the other optical elements. I know that the plane of the sample is conjugated to a plane 32mm above the top lens. The whole AFM is practically a tube about 10cm long and 1cm of diameter.

I am trying to get informations about the optical elements of a long working distance condenser. Can anyone please send me a diagram?

I think that the AFM will add one conjugate focal plane to the system. Do you think that this will be a problem?What about using reflected light (for brightfield, phase, or DIC)?

I would like any comment or idea about this.thanks a lot,daniele

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From: Frida.Maiers-at-co.hennepin.mn.us (by way of MicroscopyListserver) Date: Sat, 31 Jul 2004 21:24:27 -0400 Subject: [Microscopy] viaWWW: TEM photographic supplies available


Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Frida.Maiers-at-co.hennepin.mn.us) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, July 30, 2004 at 08:32:06---------------------------------------------------------------------------

Email: Frida.Maiers-at-co.hennepin.mn.usName: Frida Maiers

Organization: Hennepin County Medical Center

Title-Subject: [Microscopy] [Filtered] MListserver: TEM photographic supplies available

Question: Due to digital camera installation we have the following available for a nominal fee: 1 case of D19 developer, 11 boxes of EM film (Kodak 4489, new formulation), 15 liquid kits of Kodak fixer, and 17 boxes Kodak Polymax II RC photographic paper (100 sheets each). Thank you.

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From: juan-at-nanostellar.com (by way of MicroscopyListserver) Date: Sat, 31 Jul 2004 21:24:52 -0400 Subject: [Microscopy] viaWWW: Export EDX spectrum


Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (juan-at-nanostellar.com) from http://microscopy.com/MicroscopyListserver/MLFormMail.html on Friday, July 30, 2004 at 22:47:04---------------------------------------------------------------------------

Email: juan-at-nanostellar.comName: Juan

Title-Subject: [Microscopy] [Filtered] Export EDX spectrum

Question:Hi,

I took some EDX spectrum by using EDAX Phoenix 1998 Revision 3.1, but I can not export the spectrum into a data file. Does anybody know the method? Any suggestion would be highly appreciated.

Juan

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From: Gsalaj7-at-aol.com (by way of Ask-A-Microscopist) Date: Sun, 1 Aug 2004 17:36:03 -0400 Subject: [Microscopy] AskAMicroscopist: SEM of eye cyst from a swan


Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (Gsalaj7-at-aol.com) from http://www.msa.microscopy.com/Ask-A-Microscopist/Ask-A-Microscopist.html on Sunday, August 1, 2004 at 14:15:07---------------------------------------------------------------------------

Email: Gsalaj7-at-aol.comName: Sally Thomas Graziano

Organization: Orlando Science Center

Education: Undergraduate College

Location: Orlando Florida

Question: In my work on the SEM here at OSC. Along with our educational programs we have an ongoing research project. In that regard I have a very small eye cyst from a swan which I need to look at. As you can see most of my specimens are larger biological specimens. How should I mount it on the SEM stub. I use carbon tape for most things but am afraid the cyst will get lost in one of the bubbles of the tape.Also how long to coat. Any sugestions would be most helpful. Sally Graziano

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From: David Vowles : djv23-at-msm.cam.ac.uk Date: Mon, 2 Aug 2004 09:30:25 +0100 (BST) Subject: [Microscopy] Re: viaWWW: Export EDX spectrum


Juan,

You wrote:

} I took some EDX spectrum by using EDAX Phoenix 1998 Revision 3.1, but I } can not export the spectrum into a data file. Does anybody know the method? } Any suggestion would be highly appreciated.} EDAX have a free spectrum viewer that can be downloaded from: http://www.edax.com/support/EDS_Spectrum_Viewer.html

You can cut and paste the channel data from this programmme.

Alternatively, I have written an application which will display EDAX .spc files - as well as Noran, Link/Oxford and EMSA file formats - which allows export of data as well. Let me know if you want a copy.

David VowlesElectron Microscope UnitDept of Materials Science and MetallurgyUniversity of CambridgePembroke St CambridgeUK CB2 3QZTel: +44 (0)1223 334325Fax: +44 (0)1223 334567Email: djv23-at-cam.ac.uk

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From: Giles, Bill : William.Giles-at-TIMET.com Date: Mon, 2 Aug 2004 11:38:58 -0600 Subject: [Microscopy] Metallographic Sample Storage Containers


Hey Listers,




*

From: Ritchie Sims : r.sims-at-auckland.ac.nz Date: Tue, 03 Aug 2004 06:28:05 +1200 Subject: [Microscopy] Asbestos by SEM?


Hi

Can someone point me in the direction of online information regarding techniques for the identification of asbestos by SEM and by other techniques?

thanks

rtch

--Ritchie Sims Ph DPhone : 64 9 3737599 ext 87713Microanalyst Fax : 64 9 3737435Department of Geology email : r.sims-at-auckland.ac.nzThe University of Auckland Private Bag 92019AucklandNew Zealand

From: White, Woody N. : nwwhite-at-bwxt.com Date: Mon, 2 Aug 2004 15:07:59 -0500 Subject: [Microscopy] Metallographic Sample Storage Containers


Let us know if you find something that is suitable and *inexpensive*.Microscopy supply vendors sell plastic boxes, but at the rate I go throughsamples, I would go broke and run out of storage room as well.

No inexpensive vials, jars, etc. that I have found protect the preparedsurface well - no mounting method. The best compromise is to use a snuglyfitting "Cap Plug" (poly closure used to close pipe ends, other holes) andpartially insert the mount into it.


A great solution (not my idea) for 1/2" (12mm) pin-mounts is an "Opticlear"25 dram glass vial with a closed bottom poly cap. Punch or cut a snug holein the bottom (inside) of the cap, shove the stub pin in and place invial...

Regards,Woody


-----Original Message-----} From: Giles, Bill [mailto:William.Giles-at-TIMET.com] Sent: Monday, August 02, 2004 1:39 PMTo: 'Microscopy-at-microscopy.com'

Hey Listers,



William GilesSenior Electron MicroscopistMetallography Lab CoordinatorP.O. Box 2128Henderson, Nevada 89009

From: castrj01-at-endeavor.med.nyu.edu (by way of MicroscopyListserver) Date: Tue, 10 Aug 2004 08:02:33 -0500 Subject: [Microscopy] MicroscopyListserverviaWWW: siemens elmiskop 1a


Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (castrj01-at-endeavor.med.nyu.edu) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, August 10, 2004 at 07:38:02---------------------------------------------------------------------------

Email: castrj01-at-endeavor.med.nyu.edu Name: George Castro

Organization: New York University Medical Center

Title-Subject: [Microscopy] [Filtered] siemens elmiskop 1a

Question: } } I am writing in the hope that} you might refer us to someone able to repair and service a Siemens} Elmiskop 1A. I have obtained a limited amount of contacts through the} web, but with no knowledge of this scope because of its age. Perhaps} among your members there is a better chance.} I am very grateful for your time and attention.} George Castro} } George Castro} Dept. of Surgery} New York University Medical Center} 520 First Ave. #421} New York, NY 10016} Tel 212 263 6777} fax 212 263 0227} castrj01-at-popmail.med.nyu.edu}

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From: Peter Van Osta : pvosta-at-maia-scientific.com Date: Tue, 10 Aug 2004 16:36:28 +0200 Subject: [Microscopy] Discussion of a Human Cytome Project at the EMC


Hi,

Since the end of last year the idea of a Human Cytome Project has been discussed at several meetings. At the F.O.M. meeting (http://www.focusonmicroscopy.org/2004/program.html) this year the idea of a Human cytome Project was discussed in public for the first time.

The next occasion will be the European Microscopy Meeting (http://www.emc2004.be/) in Antwerp at the end of this month (Friday August 27, Session LS 18. Round table: The Human Cytome project).

I hope it will be an interesting discussion and I look forward to meet you at the meeting.

An interesting article on the idea of a Human Cytome Project:Cytomics - New Technologies: Towards a Human Cytome ProjectValet G., Trnok A.Cytometry 59A:167-171 (2004)

Some links on the topic:

http://www.biochem.mpg.de/valet/cytompr1.htmlhttp://www.biochem.mpg.de/valet/cytompr2.htmlhttp://ourworld.compuserve.com/homepages/pvosta/humcyt.htmhttp://www.cytomics.info/

Regards,

Peter Van Osta

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From: Michael Cammer : cammer-at-aecom.yu.edu Date: Tue, 10 Aug 2004 13:37:16 -0400 Subject: [Microscopy] NYTimes paean to microscopy


Dear Gerald,I have found that Abe Dayani of Refining Systems Inc. gives good prices andservice on sputtering and deposition targets of all types. He specializes intargets. You can contact him at:Refining Systems Inc.PO Box 72466Las Vegas, NV 89170phone: 702-368-0579, fax: 702-368-0933www.refiningsystems.comI am just a satisfied customer.Regards,Mary MagerElectron MicroscopistDepartment of Materials EngineeringUniversity of British Columbia6350 Stores RoadVancouver, B.C. V6T 1Z4CANADATel: 604-822-5648Fax: 604-822-3619e-mail: mager-at-interchange.ubc.ca----- Original Message ----- } From: "Gerald Bourne" {grb-at-ufl.edu} To: "Microscopy ListServer" {Microscopy-at-msa.microscopy.com} Sent: Friday, August 06, 2004 8:05 AM

Article in today's New York Times about joys of microscopy for children etc. If the link is still up:http://nytimes.com/2004/08/10/science/10essa.html?8hpib

____________________________________________________________________________Michael Cammer Analytical Imaging Facility Albert Einstein Coll. of Med.Jack & Pearl Resnick Campus 1300 Morris Park Ave. Bronx, NY 10461(718) 430-2890 Fax: 430-8996 URL: http://www.aecom.yu.edu/aif/

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From: Elaine Humphrey : ech-at-interchange.ubc.ca Date: Tue, 10 Aug 2004 12:34:22 -0700 Subject: [Microscopy] Spurr off the market


Hello EveryoneWe had a wonderful time in Savannah at the M&M meeting. Many thanks to all the organisers, vendors and particpants who made it so worthwhile for me.

But one piece of news I heard at the meeting was that Spurr resin is supposed to be going off the market next year. As far as I understand it one of the ingredients is being removed. Has anyone got any comments about this or heard of an alternative ingredient?Elaine

-- Dr. Elaine HumphreyDirector, BioImaging FacilityPresident, Microscopy Society of CanadaUniversity of British Columbia6270 University Blvd, mail-stop BotanyVancouver, BCCANADA, V6T 1Z4Phone: 604-822-3354FAX: 604-822-6089e-mail: ech-at-interchange.ubc.cawebsite: www.emlab.ubc.ca

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From: psconnel-at-sas.upenn.edu Date: Tue, 10 Aug 2004 18:45:49 -0400 Subject: [Microscopy] Re: [Microscopy: siemens elmiskop 1a


George,Peter Stolzenberg of Pesto Inc. had serviced the Siemens in the past but Ireceived word after I returned from the M&M 2004 meeting that he had recentlypassed away. He was the serviceman for my Philips 200 and I received themessage so quickly because I had requested that he do a routine for me when Ireturned. Consequently I am also looking for someone who can help me service myTEM if I get stuck.

A few years ago there was a serviceman working for JEOL whom I had first met atTemple University when he was servicing a Siemens Elmiskop there. UnfortunatelyI can not remember his name.

Pat ConnellyUniv. of PA, BiologyPhiladelphia, PA 19104-6018psconnel-at-sas.upenn.edu=========================} Organization: New York University Medical Center} Title-Subject: [Microscopy] siemens elmiskop 1a } } I am writing in the hope that} } you might refer us to someone able to repair and service a Siemens} } Elmiskop 1A. I have obtained a limited amount of contacts through the} } web, but with no knowledge of this scope because of its age. Perhaps} } among your members there is a better chance.} } I am very grateful for your time and attention.} } } } George Castro} } Dept. of Surgery} } New York University Medical Center} } 520 First Ave. #421} } New York, NY 10016} } Tel 212 263 6777} } fax 212 263 0227} } castrj01-at-popmail.med.nyu.edu

From: Terry Cooper : terry.cooper-at-btinternet.com Date: Wed, 11 Aug 2004 12:22:04 +0100 Subject: [Microscopy] Microscopy Ultramicrotome history


Dear Listers,

I am just catching up after a holiday so apologies for any delay. There hasbeen a lot of interesting info regarding early Leitz and Reichertultramicrotomes. I can add a little more to this dialogue.

During my time with Reichert-Jung UK as EM product specialist I was offeredseveral old ultramicrotomes before they were to be thrown into the Skip(Dumpster in the US?). At one time I had around a dozen of these dinosaurscluttering up my house but most of them are now thankfully residing in theArchive Building of the Science Museum in London.

Some of those I had were as follows:

Sorvall MT1 and MT2Reichert Om U1 a design by Prof. H Sitte taken up by that companyLKB Sjostrand - the original ultramicrotome from LKB on which I learnedultramicrotomyLeitz - after a design by Fernandez-Moran, a massive but beautifully madeunitPhilips - yes even this company dabbled in ultramicrotomesCambridge Rocker - a factory modified microtome for ultra thin sectioningCooke and Perkins - a simple English ultramicrotme from the fiftiesCambridge HuxleySi-ro-flex - an excellent microtome with superbly novel and advancedfeatures made by the Fairey Aviation in Australia

For those interested the first attempts to cut "ultrathin" were a bit of acheat as the idea was to cut cake-type slice from a specimen and try toimage cells at the thinnest part of the wedge. Not entirely successful.There was an early diversion with high speed microtomes in the fortiesparticularly in the USA with massive rotational speeds up to 57,000 rpm butcost and probably aerodynamics (or lack of them) led to there demise.

For anyone who might be as 'barmy' as I am with the history of these thingsI was once talked into writing a short article for "Microscopy andAnalysis". The reference is:

The Thin End of The Wedge - A personal View of The Development of TheUltramicrotome by T W Cooper, Microscopy and Analysis, January 1990.

I have no electronic copy of this dry old missive, but for anyone unable totrack it down I do still have a few reprints available that I would happilysend one to any interested party,

Best regards

Terry CooperTAAB Laboratories Equipment Ltd3 Minerva House, Calleva ParkAldermaston, Berks, RG7 8NA, EnglandTel ++44 (0)118 981 7775 Fax ++44 (0)118 981 7881e-mail sales-at-taab.co.ukwww.taab.co.uk

From: Leona Cohen-Gould : lcgould-at-med.cornell.edu Date: Wed, 11 Aug 2004 09:46:03 -0400 Subject: [Microscopy] Re: [Microscopy: siemens elmiskop 1a


So sad to hear of Peter Stolzenberg's passing. He lived and had his offices in Cold Spring Harbor, NY (on Long Island) when I was a grad student at the CW Post campus of LIU, and he kept our ancient Hitachi Hu-11A in fine running order. I got to know Peter quite well during the protracted period of time it took to track down one circuit in the HV cabinet that would short out in a very unpredictable fashion. It was always fun to be working at the scope and hear what sounded like a crack of lightening followed by total loss of HV. It was a matter of having Peter there with his meters in the right place at the right moment. Since his home was just a few miles down the road from the campus, he would stop by in the morning on his way out to other calls and then again on his way home at the end of the day. This went on for about 2 weeks or so. We were finally lucky, he found the problem and even had the right tube (yes, it was all vacuum tubes, remember those?) in stock.I learned a lot of what I know about the inner workings of an EM from Peter, and with his coaching and coaxing, got past my uneasiness about tackling mechanically-related problems with that old beast. Skills that I've carried with me.Lee-- Leona Cohen-Gould, M.S.Sr. Staff AssociateDirector, Electron Microscopy Core FacilityManager, Optical Microscopy Core FacilityJoan & Sanford I. Weill Medical Collegeof Cornell Universityvoice (212)746-6146fax (212)746-8175

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From: Leona Cohen-Gould : lcgould-at-med.cornell.edu Date: Wed, 11 Aug 2004 09:53:49 -0400 Subject: [Microscopy] Re: Microscopy Ultramicrotome history


Terry's mention of the high speed microtomes reminded me of a talk by the then semi-retired Keith Porter that I was lucky enough to attend in which he discussed the early years of EM work at the Rockefeller University. One of the things he described was just such a microtome. He said that the cutting speed was very fast, and that the sections flew off the knife ( steel, if I remember correctly) and were caught against a wire screen cage surrounding the microtome. The sections were manually retrieved from the screen.I hope I've remembered this correctly. Dr. Porter's description was so animated, it created a very vivid impression.Talk about doing things the hard way!

Lee-- Leona Cohen-Gould, M.S.Sr. Staff AssociateDirector, Electron Microscopy Core FacilityManager, Optical Microscopy Core FacilityJoan & Sanford I. Weill Medical Collegeof Cornell Universityvoice (212)746-6146fax (212)746-8175

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From: Stacie Kirsch : Stacie-at-ems-secure.com Date: Wed, 11 Aug 2004 10:01:49 -0400 Subject: [Microscopy] [Norton AntiSpam] Spurr off the market


Good Morning Elaine and all other microscopy listservers whom areinterested in the Spurrs resin and its "whereabouts". Although it istrue that one of the constituents is being dwindled from the marketERL-4206 (due to safety issues) for the past 2 years all of ourScientific team at Electron Microscopy Sciences has been doingcomparitive testing of like and similar resins to find the one that bestworks as the original. Over the 2 years of testing we have been able tocome up with one and modify it to the point where it is basicallyundistinguishable from the original 4206. We call it the ERL4221(catalog number EMS 15004). Formulas do not have to change and the4221 will become an exact replacement fo the 4206 and none of us willever know the difference. Blocks, sectioning, staining all will remianunchanged. So this is the good news. At this time there is no bad newsat all.If you have any questions or I may be of any assistance please do nothesitate to contact us. We look forward to hearing from you

Sincerely

Stacie KirschElectron Microscopy SciencesTel: 215-412-8400Fax: 215-412-8450E-mail: sgkcck-at-aol.comWebsite: www.emsdiasum.com-----Original Message-----} From: Elaine Humphrey [mailto:ech-at-interchange.ubc.ca] Sent: Tuesday, August 10, 2004 3:34 PMTo: microscopy-at-msa.microscopy.com

Hello EveryoneWe had a wonderful time in Savannah at the M&M meeting. Many thanks to all the organisers, vendors and particpants who made it so worthwhile for me.

But one piece of news I heard at the meeting was that Spurr resin is supposed to be going off the market next year. As far as I understand it one of the ingredients is being removed. Has anyone got any comments about this or heard of an alternative ingredient? Elaine

-- Dr. Elaine HumphreyDirector, BioImaging FacilityPresident, Microscopy Society of CanadaUniversity of British Columbia6270 University Blvd, mail-stop BotanyVancouver, BCCANADA, V6T 1Z4Phone: 604-822-3354FAX: 604-822-6089e-mail: ech-at-interchange.ubc.cawebsite: www.emlab.ubc.ca

From: Kathleen Roberts : kgrobert-at-rci.rutgers.edu Date: Wed, 11 Aug 2004 10:48:42 -0400 Subject: [Microscopy] Re: Re: [Microscopy: siemens elmiskop 1a


Oh, I'm sorry to hear that! Mr. Stolzenberg was just here a couple of weeks ago to service our old Zeiss EM 10-he did a great job. Please send my condolences.

Does anyone know of anyone who can service our Zeiss EM 10CA? It's OK now, but we will certainly need someone in the future.

Thanks,Kathleen RobertsPrincipal Lab TechnicianNeurotoxicology LabsDept. of Pharmacology & ToxicologyErnest Mario School of PharmacyRutgers University41 B Gordon RdPiscataway, NJ 08854

psconnel-at-sas.upenn.edu wrote:


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From: David Henriks : henriks-at-southbaytech.com Date: Mon, 02 Aug 2004 14:23:34 -0700 Subject: [Microscopy] Re: Metallographic Sample Storage Containers


Colleagues

I have spoken with Eric Ambrose concerning this message. In my opinion itis not an advertisement and is definitely of interest and importance to the Microscopy Community.

Eric , thank you again for first checking with me on this.

NestorYour Friendly Neighborhood SysOp

----------------------------------------------------

Bill:

We have some containers that are ideal for this application. You can see an image of them on our website at www.southbaytech.com. Do a keyword search for "containers". You will also find them listed with our extensive range of metallographic consumables under the "consumables" button. I will contact you off-line with pricing.

Best regards-

David

Giles, Bill wrote:


-- David HenriksVice President







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From: Gregory Mulhollan : gmulhollan-at-austin.rr.com Date: Mon, 2 Aug 2004 16:38:51 -0500 Subject: [Microscopy] Seeking manual for TN6500


Hello Microscopy folks,I am trying to resurrect an old spectroscopy system that uses a Tracor Northern TN-6500 box for controlling a linear diode readout. Anyone out there with a manual for the TN-6500? I am happy to pay photocopy charges or do anything (reasonable!) to get my hands on one. Thanks again.Greg Mulhollan-----------------------------------Gregory Mulhollan, Ph.D.Saxet Surface Science1001 S. Sunset Canyon DriveDripping Springs, TX 78620(512)858-2841 office(512)694-4879 cellmulhollan-at-saxetsurfacescience.comwww.saxetsurfacescience.com

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From: Garber, Charles A. : cgarber-at-2spi.com Date: Tue, 03 Aug 2004 02:10:03 -0500 Subject: [Microscopy] Storage containers for 1 metallographic mounts


-- [ From: Garber, Charles A. * EMC.Ver #3.1 ] --

William Giles wrote:==========================================================================================We are looking for a source of individual metallographic sample storagecontainers. Our mounts are typically 1" diameter and 0.5-1" tall.

Anyone have a source for these?==========================================================================================SPI Supplies has manufactured for some years our special mount storage boxfor 1" mounts, see URLhttp://www.2spi.com/cataelog/boxes/speci_box.shtml

This would be our SPI #02020-AB and each one holds eight mounts. There areinserts for desiccating capsules. And once the samples are loaded into thebox, and the lid closed, some ordinary Scotch Tape is put around the lip ofthe closed box to seal out air or anything else that might seep in anddamage samples.

Disclaimer: SPI Supplies is the manufacturer of this special kind ofstorage box.

Chuck

===================================================Charles A. Garber, Ph. D. Ph: 1-(610)-436-5400President 1-(800)-2424-SPISPI SUPPLIES FAX: 1-(610)-436-5755PO BOX 656 e-mail: cgarber-at-2spi.comWest Chester, PA 19381-0656 USA Cust. Service: spi2spi-at-2spi.com

Look for us! ############################ WWW: http://www.2spi.com ############################==================================================

From: Giles, Bill : William.Giles-at-TIMET.com Date: Tue, 3 Aug 2004 07:51:12 -0600 Subject: [Microscopy] Re: Met Sample containers


Thanks for all the responses; I'll filter thru all the suggestions.


*

From: Szewczyk, Steven (Cont, ARL/WMRD) : sszewczyk-at-arl.army.mil Date: Tue, 3 Aug 2004 10:44:38 -0400 Subject: [Microscopy] Metallographic Sample Storage Containers


We use the "Cap Plugs" Woody has suggested and I find they work well ifyou're looking for inexpensive (a few cents per piece) individual samplestorage.

The caps come in a variety of shapes and sizes, and I'm sure there aremany sources for such items. The vendor we ordered from has a good pagewith drawings and dimensions for each:http://www.niagaracapsandplugs.com/nonthreaded_caps.htm

Regards,

Steven T SzewczykMaterials Science ContractorUS Army Research LabAberdeen Proving Ground, MD

-----Original Message-----} From: White, Woody N. [mailto:nwwhite-at-bwxt.com] Sent: Monday, August 02, 2004 4:08 PMTo: 'Giles, Bill'; 'Microscopy-at-microscopy.com'

Let us know if you find something that is suitable and *inexpensive*.Microscopy supply vendors sell plastic boxes, but at the rate I gothrough samples, I would go broke and run out of storage room as well.

No inexpensive vials, jars, etc. that I have found protect the preparedsurface well - no mounting method. The best compromise is to use asnugly fitting "Cap Plug" (poly closure used to close pipe ends, otherholes) and partially insert the mount into it.


A great solution (not my idea) for 1/2" (12mm) pin-mounts is an"Opticlear" 25 dram glass vial with a closed bottom poly cap. Punch orcut a snug hole in the bottom (inside) of the cap, shove the stub pin inand place in vial...

Regards,Woody


-----Original Message-----} From: Giles, Bill [mailto:William.Giles-at-TIMET.com]Sent: Monday, August 02, 2004 1:39 PMTo: 'Microscopy-at-microscopy.com'

Hey Listers,




*

From: George Langford, Sc.D. : amenex-at-amenex.com Date: Tue, 3 Aug 2004 11:40:28 -0400 (EDT) Subject: [Microscopy] Re: Commercial SEM labs in the Cleveland, OH area ?


Hello Microscopists !

Quite some time ago, in anticipation of a congenial meeting of interestedparties at a common-ground investigation of several broken steel bolts inthe Cleveland, Ohio, area, I asked about suitable SEM labs in the area.I received an excellent response from The List, which indicates that it isthe primary means of rapid and effective communication in the micrsocopycommunity. Alas, there was a slower response from the above-referencedinterested parties, and so no agreement about protocol or venue wasreached until we had all retired to our own offices. Later on, we wound upgoing back to the place selected originally by one of the parties way backat the beginning of the project, where 1000X is about the limit of usefulmagnification. I am sure any one of you could have done better.

Thanks to all who reponded.

Best regards,George Langford, Sc.D.Principal ConsultantAmenex Associates, Inc.amenex-at-amenex.comhttp://www.amenex.com/

From: elliott : elliott-at-uark.edu Date: Tue, 03 Aug 2004 14:39:33 -0500 Subject: [Microscopy] Wehnelt cleaning


Mr. Chapman,When you say "stock ammonia solution", are you talking about standard Ammonia(NH3)?

From: garyeaston-at-scannerscorp.com (by way of MicroscopyListserver) Date: Tue, 3 Aug 2004 19:51:53 -0400 Subject: [Microscopy] viaWWW: Cambridge/Leica/LEO S360 SEM


Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (garyeaston-at-scannerscorp.com) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, August 3, 2004 at 13:34:53---------------------------------------------------------------------------

Email: garyeaston-at-scannerscorp.comName: Gary M. Easton

Organization: Scanners Corporation

Title-Subject: [Microscopy] [Filtered] Cambridge/Leica/LEO S360 SEM

Question: I just picked up a used Cambridge S360 that gives me an error code 307(defective LAB6 switch). Does anyone out there that has a complete list of the software error codes and their explanation? The operator's manual offers no help. Also, if anyone has the setup software (mag cal,etc) for this series SEM and is willing to part with a copy, it would be greatly appreciated. Please reply offline to garyeaston-at-scannerscorp.com. Thanks in advance.

Gary M. Easton

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From: cnorton-at-wis-inc.net (by way of MicroscopyListserver) Date: Tue, 3 Aug 2004 19:52:30 -0400 Subject: [Microscopy] viaWWW: searching for service engineers


Below is the result of your feedback form (NJZFM-ultra-55). It was submitted by (cnorton-at-wis-inc.net) from http://www.msa.microscopy.com/MicroscopyListserver/MLFormMail.html on Tuesday, August 3, 2004 at 15:36:41---------------------------------------------------------------------------

Email: cnorton-at-wis-inc.netName: chris norton

Organization: Wafer Inspection Services, Inc.

Title-Subject: [Microscopy] [Filtered] MListserver:

Question: We are searching for service engineers with KLA-Tencor service and applications experience on all models.Prometrix UV1250/1280; RS 75; 6420; AIT; Starlight; 8100 CD SEMS; etc.

---------------------------------------------------------------------------

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From: David Elliott : David.Elliott-at-yale.edu Date: Wed, 4 Aug 2004 08:09:17 -0700 Subject: [Microscopy] Leica ultramicrotome



_____________________


Voice: 520-626-7870Fax: 520-626-2097

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From: Ann St. Amand : astamand-at-phycotech.com Date: Wed, 04 Aug 2004 11:57:12 -0400 Subject: [Microscopy] Need to borrow or rent 11 LM Phase scopes for a workshop in


Hi all, I am co-chair of a workshop being given on algae identification at the North American Lakes Management Society International Symposium in Victoria Canada. The workshop is being offered November 2, 2004. Normally, its not a problem to get the scopes, but I can't find a source in Canada. I need 10 teaching scopes with Phase and 400 objectives and 1 BX60 with Phase at 200 and 400 with a trinoc head and a digital camera. Can anyone help? I'm located in Michigan, United States. Thanks much, Ann.

Ann St. Amand, Ph.D.PresidentPhycoTech, Inc.620 Broad St., Suite 100St. Joseph, Michigan 49085 USA

269-983-3654 (voice)269-983-3653 (fax)mailto:astamand-at-phycotech.comwww.phycotech.com

Specializing in Aquatic Sample Analysis and Microscope Accessories

Director, Region V, North American Lake Management Societ

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