observe

be inspired

reveal

APPROVED

Who said stress could be productive ?In the case of our adipocytes stress in fact goes

with the production…of fat.

Without really noticing it, the externalenvironment is often hostile for our skin.Repeated and daily exposure to exhaustfumes, UV radiation, pesticides, cigarettesmoke…generates oxidative stress in ourskin. It causes the production of freeradicals, veritable cellular missiles whichattack our cells, oxidise proteins andmembrane lipids, degrade DNA, disturbnormal cell function, and accelerate their

ageing.

Free radicals are implicated in fat storage through several ways:

• Alteration of the working order of adipocytes which become insensitive tolipolytic agents (whether produced by the organism or supplied by slimming

treatments)• Stimulation of the production of prostaglandins, especially PGE2 which favours

the production of VEGF, the development of blood vessels and therefore thesupplying of oxygen and fatty acids to adipocytes.

• The decrease in the synthesis of adipokin, especially adiponectin, a moleculeresponsible for the limitation of the expansion of adipose tissue.

At this stage, the adipocytes lose their lipolytic activity and continue to store fatswithout ever hydrolysing them.

Lotusmaritimus

Still called “PeaLotus” (Dragon’sTooth or SeaBirdsfoot Trefoil) itgrows particularly in wetregions near the seaacross all of western Europebut it is also found inland,including in the mountains up to 1600maltitude.

It is a small vigorous plant with a woody taproot and a horizontal stem which thenstraightens up. Its flowers are goldenyellow giving it the name “Golden Slipper”,or “Bride’s Slipper” in France.

Lotus has calming properties, in thepast it was given to reduce anxiety,nervousness and muscular spasms.Some use the flowers as a sleepingdraught. It was also taken to be themagic plant which made Ulysses’companions lose their memory in theOdyssey.

For the more mystical amongst us,Lotus corniculatus assistspsychic powers and helpsclairvoyance. An ancientSwedish tradition says thatyoung betrothed who putLotus flowers under theirpillows on Midsummer’sNight will have revealed tothem the face of theirfiancé.

OBSERVE

BE INSPIRED

Fat storage Fat Hydrolysis = Fat Hydrolysis Fat storage

Healthy tissue Cellulite

oxydativestress

Ageing

After several years research on the action of free radicals, CODIF Recherche et Nature hasdeveloped Hydrofiltrat Marine Lotus G. By protecting our adipocytes from oxidative stress and free radicals, it prevents loss offunctionality, delays ageing, restores lipolytic activity and boosts caffeine’s lipolytic effects.Deprived of its best allies, cellulite disappears and our silhouette regains harmony and beauty.

Adipocytes are generally considered to have the capacity tostore fatty acids. However they possess all the meansnecessary to hydrolyse fats, this mechanism being calledLipolysis. Lipolysis is induced by internal factors such asadrenaline, but can also be amplified by external lipolytic suchas caffeine.

APPROVEDHYDROFILTRAT MARINE LOTUS GOXIDATIVE STRESS…

OUR WORST ENEMY FOR CELLULITE.

2 factors are mainly involved in lipolysis :

• cAMP (cyclic Adenosine MonoPhosphate), which issynthesised from ATP and activates lipase byphosphorylation,• lipase which once it is activated catalyses triglyceridehydrolysis into fatty acids and glycerol.

cAMP is therefore at the heart of lipolysis and is the firsttarget of agents which activate or inhibit this mechanism.Phosphodiesterase (PDE) inhibits cAMP, while adrenalinefixation on its beta receptor stimulates its activity.

One of the first targets of free radicals in the cell is the mitochondrion. When they are damaged,mitochondria produce less ATP, the cAMP concentration falls and the lipolytic activity is therefore reduced.By protecting adipocytes and their mitochondria from oxidative stress, Hydrofiltrat Marine Lotus Gmaintains an optimal level of cAMP and restores the efficacy of lipolytic agents.

Oxidative stress and ageing are accompanied by a loss of adipocyte sensitivity to lipolyticagents, and therefore a reduction in lipolysis.Protecting the adipocytes from free radicals forearms them against any celllular dysfunctioning,delays their ageing, and therefore maintains and reinforces the effectiveness of lipolytic agentslike caffeine in slimming treatment.

200

400

600

800

1000

1200

-49%

-70%

0Isoproterenol

Effect of adipocytes ageing on the efficacy of lipolytic agents

35-40 years old 40-50 years old 50-60 years old

Re

sp

on

se

to

lip

oly

sis

( μM

fa

tty a

cid

lib

era

te

d)

Caffeine

-20%

-60%

source CODIF International

FattyAcids

+Glycerol

Triglycerides

Lipase

Lipase

AMPc

PDE

2

1

-

Adrenaline

+

β

In vitro, the lipolytic action of adrenaline on adipocyte cultures is recreated using isoproterenol ornoradrenaline. Like adrenaline, these two lipolytic agents fix on the β adrenergic receptor and stimulatethe hydrolysis of fatty acids. Caffeine, a lipolytic agent widely used in cosmetics, inhibits PDE, removing theinhibition previously exercised by cAMP.

Effect of ageing and oxidative stress on lipolysis

Effect of oxidative stress on the efficacy of lipolytic agents:

concentration in Noradrenaline (mol/L)

Lip

oly

sis

(μg

/m

illio

n c

ell.h

ou

r)

Oxidising stress generated by addition of glucose oxidase

1

2

3

4

5

6

0

Source INSA, Lyon

Control

Stress

10-9 10-8 10-7,3 10-7 10-6,3 10-6

2000

4000

6000

8000

10000

12000

12300

1669

396

0

Hydrofiltrat MarineLotus G

ORAC Test

OR

AC

(μm

ol T

E/

L)

Cranberry1%

Quercetin1%

20

40

60

80

100

120

140

160

141148

94

0

Hydrofiltrat MarineLotus G

DPPH Test

DP

PH

(μm

ol T

E/

L)

Cranberry1%

Quercetin1%

•ORAC (Oxygen Radical AbsorbanceCapacity) : overall measure ofantioxidant capability, •DPPH : complementary test toORAC, antioxidant capacitycorrelated to polyphenol content.

Reactions standardised in relationto known concentrations of TROLOX(the water soluble equivalent ofVitamin E)Cranberry and Quercetin are usedas positive controls.

Hydrofiltrat Marine Lotus G has very high antioxidant effect.

In-vitro Test : antioxidant properties of Hydrofiltrat Marine Lotus G

MEMO ON CUTANEOUS BIOLOGY…

…THE LIPOLYTIC AGENTS

TE = Trolox Equivalence

In-vitro Test :effect of Hydrofiltrat Marine Lotus G 2% on adipocyte lipolytic activity

Protocol :Culture of mature adipocytes with or without oxidativestress in presence or absence of Hydrofiltrat MarineLotus G 2% for 1 week.

Incubation in presence of lipolytic agents ( isoproterenoland caffeine)for 5 hours

Measure of lipolytic activity (amount of glycerol liberatedby lipolysis)

Thanks to its strong antioxidant capacities, HydrofiltratMarine Lotus G neutralizes free radicals, restores thelipolytic activity of isoproterenol and boosts the efficacyof caffeine.

By protecting fibroblasts from oxidative stress, Hydrofiltrat Marine Lotus G limits thesecretion of PGE2 and therefore reduces the supplying of fatty acids to adipocytes.

Protocol :Culture of adipocytes with Hydrofiltrat Marine Lotus G for 7days, followed by UV exposure (oxidative stress).

Adiponectin is a factor released by adipocytes themselves,which limits the expansion of the adipose tissue. Under theeffect of oxidative stress, its production is significantlyreduced.

First of all, Hydrofiltrat Marine Lotus G has astimulating effect on the synthesis of adiponectin,then, thanks to its antioxidant capacities, it has aprotective effect when adipocytes are submitted tooxidative stress.These both complementary actions limit in apermanently way the expansion of the adipose tissue.

HMLG=Hydrofiltrat Marine Lotus G

In-vitro Test : effect of Hydrofiltrat Marine Lotus G 2% on the release of PGE2

The factor PGE2 (ProstaGlandin E2) isreleased by fibroblasts under stressfulconditions. PGE2 favours the production of VEGFwhich stimulates the development ofblood vessels and therefore the supplyingof oxygen and fatty acids to adipocytes.

Protocol :Culture of fibroblasts with HydrofiltratMarine Lotus G for 24 hours before andafter UV exposure.

50

100

150

200

250

100 100

48 54*

101**

225**

0

Without oxidative stress

Oxidative stress

Response to isoproterenol

Response to caffeine

*p<0.05 (student test)**p<0.01 (student test)

Lip

oly

tic

Ac

tiv

ity

(% o

f c

on

tro

l)

Oxidative Stress + HydrofiltratMarine Lotus G

2%

50

100

150

200

250

300

350

400

450

100

407

357

202**

0

Without Oxidative Stress

With OxidativeStress

** p<0.01 (Student test)

Re

lea

se

of

PE

G2

(% o

f th

e c

on

tro

l w

ith

ou

t o

xid

ativ

e s

tre

ss

)

Oxidative StressHydrofiltrat

Marine Lotus G 2%

Oxidative StressHydrofiltrat

Marine Lotus G 4% + +

20

40

60

80

100

120

140

160

100

Stimulating effect

Protective effect

124*

141*

76*83

89**

0

Withoutoxidative

stress

Withoutoxidative

stress+HMLG

1%

Withoutoxidative

stress+HMLG

2%

Withoxidative

stress

With oxidative

stress+HMLG

1%

With oxidative

stress+HMLG

2%

*p<0.05 **p<0,01 (Student Test)

Ad

ipo

ne

ctin

syn

th

es

is(%

of

th

e c

on

tro

l w

ith

ou

t o

xid

ativ

e s

tre

ss

)

In-vitro Test : effect of Hydrofiltrat Marine Lotus G 2% on the production and the protection of adiponectin

INCI : Glycerine (and) Water (and) Lotus maritimus extract

Land plant origin Hydrosoluble active ingredient in-vitro Test in-vivo Test ECOCERT validation

Protocol :• 15 volunteers between 30 and 50 years old• 2 applications daily for 28 days on thighs, hips and buttocks

Evaluation of slimming effectiveness :• fringe projection• measure of loss in centimetres• evaluation in a cosmetic of cellulite by macro-photography

FRINGE PROJECTION

CENTIMETRE LOSS AND VISIBLE REDUCTION IN CELLULITE

The strong antioxidant effect of Hydrofiltrat Marine Lotus G protects adipocytes from thenegative effects of free radicals. It restores the lipolytic capacity of fat cells, limits the supplyingof fats to adipocytes, controls the expansion of the adipose tissue and strengthens the slimmingproperties of caffeine. Used alone as a 2% solution or in combination with another slimming agent such as caffeine,Hydrofiltrat Marine Lotus G removes unsightly curves, sharpens the silhouette and removescellulite.

Significant decrease in the volume of thebuttocks observed by 73% of volunteers:-0.6% on average (p<0.01) and up to-2.65%

Significant decrease in the perimeter of thethighs observed by 73% of volunteers: -0.5%on average (p<0.01) and up to -1,8%.Significant decrease in the volume of thebuttocks by -4,4% (-0,9% on average).

A statistically significantcentimetre loss is measuredon the thighs, the buttocksand the hips, accompanied bya smoothing effect of theorange peel aspect.

Clinical Test : slimming effect of Hydrofiltrat Marine Lotus G 2%

REVEAL

Average and optimum centimetre losses

Thighs

Buttocks

Hips

Centimetric lost (cm) * p<0,001 / ** p<0,01

0

0,2

**0,1 0,4

*0,3

*0,3 0,7

0,8

0,4 0,6 0,8 1

D0

D0

D28

APPROVED

D0D28

-1,8%-2,65%

Controled management program ofnatural resources

HYDROFILTRAT MARINE LOTUS G

Cosmetic Activities

INCI

% concentration recommended

ANTIOXIDANT-SLIMMING

• Anti-free radical activity – Anti-inflammatory activity• Restoration of lipolytic activity• Stimulation and protection of adiponectin • Reinforcement of caffeine efficacy• Reduction in buttock and thigh volume• Elimination of orange peel effect

Glycerine (and) Water (and) Lotus maritimus extract

2%

Technical department : Codif Recherche & Natureinfotech@codif-recherche-et-nature.com

Commercial department: Codif Recherche & Naturecommercial@codif-recherche-et-nature.com

70, rue du Commandant l’Herminier - BP11709 - 35417 Saint-Malo cedex - FRANCETel : +33-2-23-18-31-07 Fax : +33-2-23-18-31-01

www.codif-recherche-et-nature.com

CODIF Recherche et Nature is committed to sustainable development.

All our wastewater is processed and purified using filtrating gardens.A succession of airtight horizontal and vertical filters is used to depollute wastewater by the

roots of plant species chosen for their draining properties.

Iris, water mint, flowering rush, willows etc… now form part of the image of the brand which isrecognized for its commitment to the preservation of natural resources R

PD

OC

087G

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