mite preparations for identifications - biodiversity mite...preservation mite stored in alcohol mite...

Click here to load reader

Post on 05-Jul-2018

219 views

Category:

Documents

2 download

Embed Size (px)

TRANSCRIPT

  • Mite preparations for identifications

    Day 3

    Felicity Crotty

  • Collection Most common method for

    mite collection is the use of Tullgren funnels.

    Although others possible floatation / pooters.

    Collection / storage in 70% alcohol best method (unless want to further experiment)

    Saturated salt solution also used

  • Preservation Dissecting microscope to

    sort through fauna

    Compound microscope to observe external structures for key

    Highly sclerotised mites need to be cleared and disected before mounting on slides

    This is to make permanent slides

  • Preservation Mite

    stored in alcohol

    Mite soaked overnight in 90%

    lactic acid (on warmer)

    Mite placed on slide in PVA, coverslip

    edges sealed with DPX

    Dissecting microscope to sort through fauna

    Compound microscope to observe external structures for key

    Highly sclerotised mites need to be cleared and disected before mounting on slides

    This is to make permanent slides

  • Identification What level?

    - Order (Collembola or Mite)

    - Lineage (Mesostigmata/Oribatida)

    - Supercohort

    (Macropyline/Brachypyline)

    - Cohort (Palaeosomata/Mixonomata)

    - Superfamily (Phthiracaroidea/Lohmannioidea)

  • Methods of identification

    Computer based key Lucid

    Available online

    Major mite taxa home page

    http://itp.lucidcentral.org/id/mites/invasive_mite/Invasive_Mite_Identification/key/Major_Mite_taxa/Media/Html/Home_Major_Mite_Taxa.html

    Unfortunately doesnt cover that much

    Order: Opilioacarida, Holothyrida, and Ixodida; Suborder: Mesostigmata;

    Supercohort: Oribatida and Prostigmata.

    http://itp.lucidcentral.org/id/mites/invasive_mite/Invasive_Mite_Identification/key/Major_Mite_taxa/Media/Html/Home_Major_Mite_Taxa.htmlhttp://itp.lucidcentral.org/id/mites/invasive_mite/Invasive_Mite_Identification/key/Major_Mite_taxa/Media/Html/Home_Major_Mite_Taxa.htmlhttp://itp.lucidcentral.org/id/mites/invasive_mite/Invasive_Mite_Identification/key/Major_Mite_taxa/Media/Html/Home_Major_Mite_Taxa.htmlhttp://itp.lucidcentral.org/id/mites/invasive_mite/Invasive_Mite_Identification/key/Major_Mite_taxa/Media/Html/Home_Major_Mite_Taxa.html

  • Different Lucid Key

    Greater scope

    - COHORT

    Mesostigmata, Oribatid and Prostigmata.

    http://keys.lucidcentral.org/key-server/player.jsp?keyId=42

    http://keys.lucidcentral.org/key-server/player.jsp?keyId=42http://keys.lucidcentral.org/key-server/player.jsp?keyId=42http://keys.lucidcentral.org/key-server/player.jsp?keyId=42http://keys.lucidcentral.org/key-server/player.jsp?keyId=42http://keys.lucidcentral.org/key-server/player.jsp?keyId=42http://keys.lucidcentral.org/key-server/player.jsp?keyId=42http://keys.lucidcentral.org/key-server/player.jsp?keyId=42

  • Dichotomous Keys

    Used Tiling Key

    Paired statements of either words or images

    Have to follow specific order

    If character unknown / cant see it easy to make a mistake and misidentify

  • Dichotomous keys

    Greater depth

    Orders

    Lineages

    Families

    Genus

    Species

  • Acari

    Parasitiformes Acariformes

    Sub-Class

    Super-Order

    Order Opilloacarida Holothrida Mesostigmata Ixodida Sarcoptiformes Trombidiformes

    Sub-Order Sejida

    Trigynaspida Monogynaspida Endeostigmata

    Oribatida

    Sphaerolichida

    Prostigmata

    Super-Cohort

    Palaeosomata

    Parhyposomata

    Brachypylina Enarthronota

    Mixonomata Desmonomata

    Cohort

    Cercomegistina

    Antennophorina

    Microgynina

    Heatherellina

    Heterozerconina

    Uropodina

    Gamasina

    Astigmata

    Labidostommatides

    Eupodides

    Anystides Eleutherengonides

    Anystina

    Parasitengonina

    Raphignathina

    Heterostigmata

    Sub-Cohort / Infra-Order

    Arctacariae

    Parasitiae

    Epicriiae

    Dermanyssiae

    Pycnonticae

    Poronoticae

    Euptyctima

    Dichosomata

  • Trachytidae; Dithinozerconidae; Polyaspididae; Protodinychidae; Dinychidae; Trachyuropodidae; Trematuridae; Oplitidae; Metagynellidae; Uropodidae

    Microgyniidae

    Epicriidae; Zerconidae

    Parasitidae Antennophorida; Celaenopsidae Veigaiidae; Ologamasidae;

    Rhodacaridae; Digamasellidae; Macrochelidae; Pachylaelapidae; Eviphididae; Podocinidae; Laelapidae; Amerosidae; Phytoseidae; Ascidae

    Sejida

  • Euphthiracarida; Phthiracaridae; Oribotritiidae; Oribotritiidae; Hydrozetidae; Peloppiidae; Autognetidae; Ctenobelbidae; Suctobelbidae; Caleremaeidae; Micreremidae; Amerobelbidae; Oppiidae; Scutoverticidae; Cepheidae; Tectocepheidae; Carabodidae; Thyrisomidae; Astegistidae; Cepheidae; Quadroppidae; Hermanneiellidae; Damaeidae; Poroliodidae; Eremaeidae; Ameronothridae; Damaeolidae; Cymbaeremaeid; Galumnidae; Microzetidae; Achipteriidae; Haplozetidae; Phenopelopidae; Oribatellidae; Ceratozetidae; Euzetidae; Schleloribatidae; Oribatulidae; Zetomimidae; Limnozetidae; Humerobatidae; Mycobatidae; Chamobatidae; Passlozetidae; Licneremaeidae; Enichthoniidae; Hypochthoniidae; Atopochthoniidae; Cosmochthoniidae; Brachychthoniidae; Gehypochthoniidae; Liacaridae; Gustaviidae; Malaconothrida; Trhypochthoniidae; Eulohmanniidae; Perlohmanniidae; Epilohmanniidae; Nanhermanniidae; Hermanniidae; Camisiidae; Nothridae; Adelphacaridae; Palaeacaridae

  • Euphthiracarida; Phthiracaridae; Oribotritiidae; Oribotritiidae; Hydrozetidae; Peloppiidae; Autognetidae; Ctenobelbidae; Suctobelbidae; Caleremaeidae; Micreremidae; Amerobelbidae; Oppiidae; Scutoverticidae; Cepheidae; Tectocepheidae; Carabodidae; Thyrisomidae; Astegistidae; Cepheidae; Quadroppidae; Hermanneiellidae; Damaeidae; Poroliodidae; Eremaeidae; Ameronothridae; Damaeolidae; Cymbaeremaeid; Galumnidae; Microzetidae; Achipteriidae; Haplozetidae; Phenopelopidae; Oribatellidae; Ceratozetidae; Euzetidae; Schleloribatidae; Oribatulidae; Zetomimidae; Limnozetidae; Humerobatidae; Mycobatidae; Chamobatidae; Passlozetidae; Licneremaeidae; Enichthoniidae; Hypochthoniidae; Atopochthoniidae; Cosmochthoniidae; Brachychthoniidae; Gehypochthoniidae; Liacaridae; Gustaviidae; Malaconothrida; Trhypochthoniidae; Eulohmanniidae; Perlohmanniidae; Epilohmanniidae; Nanhermanniidae; Hermanniidae; Camisiidae; Nothridae.

    Canestriniidae; Histiostomatidae; Winterschmidtiidae; Glycophagidae; Acaridae

  • Euphthiracarida; Phthiracaridae; Oribotritiidae; Oribotritiidae; Hydrozetidae; Peloppiidae; Autognetidae; Ctenobelbidae; Suctobelbidae; Caleremaeidae; Micreremidae; Amerobelbidae; Oppiidae; Scutoverticidae; Cepheidae; Tectocepheidae; Carabodidae; Thyrisomidae; Astegistidae; Cepheidae; Quadroppidae; Hermanneiellidae; Damaeidae; Poroliodidae; Eremaeidae; Ameronothridae; Damaeolidae; Cymbaeremaeid; Galumnidae; Microzetidae; Achipteriidae; Haplozetidae; Phenopelopidae; Oribatellidae; Ceratozetidae; Euzetidae; Schleloribatidae; Oribatulidae; Zetomimidae; Limnozetidae; Humerobatidae; Mycobatidae; Chamobatidae; Passlozetidae; Licneremaeidae; Enichthoniidae; Hypochthoniidae; Atopochthoniidae; Cosmochthoniidae; Brachychthoniidae; Gehypochthoniidae; Liacaridae; Gustaviidae; Malaconothrida; Trhypochthoniidae; Eulohmanniidae; Perlohmanniidae; Epilohmanniidae; Nanhermanniidae; Hermanniidae; Camisiidae; Nothridae.

    Canestriniidae; Histiostomatidae; Winterschmidtiidae; Glycophagidae; Acaridae

    Alicohagiidae; Nanorchestidae; Alycidae; Terpnacaridae

  • Labidostommatidae; Cryptognathidae; Eriophyoidea; Penthalodidae; Scutacaridae; Tarsonemidae; Pygmephoridae; Acarophenacidae; Calyptostomatidae; Microtrombidiidae; Smarididae; Erythraeidae; Tanaupodidae; Johnstonianidae; Eutrombidiidae; Trombidiidae; Trombidulidae; Anystidae; Cheyletidae; Stigmaeidae; Bdellidae; Cunaxidae; Eupodidae; Penthaleidae; Rhagidiidae; Erynetidae; Tydaeidae; Iolnidae; Triophytdaeidae;

  • Basic mite taxonomy

    Day 3

    Felicity Crotty

  • Soil Mesofauna = Springtails and Mites Mites (Acari)

    Three main orders

    Mesostigmata

    Oribatids

    Prostigmata

    45,000 described spp

    Approx 64 spp in 1 grassland

  • Stable Community Composition: Mites

    11%

    23%

    35%

    31%

    Diversity mites in grassland (+/- 0.05 s.e.)

    ASTIGMATA MESOSTIGMATA

    ORIBATID PROSTIGMATA

    16%

    14%

    61%

    9%

    Diversity of mites in willow woodland

    (+/- 0.05 s.e.)

    ASTIGMATA MESOSTIGMATA

    ORIBATID PROSTIGMATA

  • Oribatid

  • Soil Mite or Tick?!

    Mesostigmata Ixodida

    Parasitiformes

  • Soil Mite or Tick?!

    Mesostigmata Ixodida

    Parasitiformes

    - Smaller than ~ 5mm - Peritremes and stigmatal opening

    present (at level of coxae II-IV) - Four pairs of setae on subcapitulum - Apotele with 2 or 3 tines - Tritosternum present - Female covered by 1-4 epigynal

    shields

    - Greater than ~5mm - Peritremes and stigmatal opening

    absent - Subcapitulum with denticulate

    hypostome - Palps usually 4 or fewer segments,

    without apotele - Tritosternum absent

  • Soil Mite or Tick?!

    Mesostigmata Ixodida

    Parasitiformes

  • Acari Key to main groups

    Stigmata usually without noticeable peritremes, and not situated at each side of the body near coxae I-IV. FEMALES: Genital shield jointed to open at one lateral edge of shield ..(next)

    Stigmata, one on each side of the body, situated in the region of co