Mitosis Mix up activity

WATCH THIS FIRST:Chi Square video link

Normal untreated Onion root tip

Now let’s look at a different case

You should have done this yesterday

• Complete the mitotic mix up activity chart portion for both tissue samples

• Now we know what the normal percentage of time a ORT cell spends in each stage of mitosis

• We should have a percentage of cells in each stage for the caffeine treated and the untreated cells

1/12:Step by step

1. Label a page in your lab notebook: the effects of caffeine mitosis.

2. Do you think caffeine will effect the rate of mitosis?3. Let’s go with the null hypothesis.4. Write the null hypothesis for this experiment in your

notebook.5. Make sure you watched the video linked in the first

slide of this power point here it is again: https://www.youtube.com/watch?v=WXPBoFDqNVk

Observed Versus Expected

• Working from H0= Caffeine does not effect the rate of mitosis in the root tip of onion plants.

• Expected would be the normal rates(percentages) of each stage of the cell cycle

• Observed would be the percentages of cells in each stage of the cell cycle after caffeine treatment

Chi Square Chart1. Indicate what you are counting in your

notebook. Describe your brief procedure : briefly describe how you decided a cell was in each stage

2. Create the chart below and fill it inStage # observed

(O)# expected (E)

(O-E) (O-E)2 (O-E)2/E

Inter

Pro

Meta

Ana

Telo

X2=

Make a conclusion

• Determine the degrees of freedom ( you have 5 groups of data because there are 5 stages)

• Use a critical value of .05• Write a paragraph telling if you supported or

rejected your null hypothesis and why.• Insert your mitosis mix up completed • Turn this in for a major grade ( you may turn in

on Wednesday) If there are questions email me

Chi Square chart