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MoClo Plant Parts Kit

Description: This collection comprises 95 standardised parts to enable Golden Gate construction of multi-gene constructs for plant transformation. Thirty nine parts encode promoters and 5' untranslated regions; eleven parts encode antigenic tags; eight parts encode sub-cellular localisation signals; nineteen parts encode reporter genes; six parts encode selectable marker genes; nine parts encode terminators and 3' untranslated regions. A suppressor of silencing and two linkers are also included.

More information can be found at: www.addgene.org/cloning/MoClo/ www.addgene.org/cloning/MoClo/Patron/

Reference: A Golden Gate Modular Cloning Toolbox for Plants. Carola Engler, Mark Youles, Ramona Gruetzner, Tim-Martin Ehnert, Stefan Werner, Jonathan D. G. Jones, Nicola J. Patron, and Sylvestre Marillonnet ACS Synth. Biol., Just Accepted Manuscript • DOI: 10.1021/sb4001504 • Publication Date: 05 Feb 2014

Handling and Storage: Store glycerol stocks at -80oC and minimize freeze-thaw cycles. To access a plasmid, keep the plate on dry ice to prevent thawing. Using a sterile pipette tip, puncture the seal above an individual well and spread a portion of the glycerol stock onto an agar plate. To patch the hole, use sterile tape or a portion of a fresh aluminum seal.

Note: These plasmid constructs are being distributed to non-profit institutions for the purpose of basic research.

Please contact Addgene at help@addgene.org with any questions.

Plate Map

Instructions: To access a plasmid, keep the plate on dry ice to prevent thawing. Using a sterile pipette tip, puncture the seal above an individual well and spread a portion of the glycerol stock onto an agar plate. To patch the hole, use sterile tape or a portion of a fresh aluminum seal. !

MoClo Plant Parts Kit

Please visit www.addgene.org/cloning/MoClo/Patron/ for a list of the appropriate antibiotics for each plasmid.

1 2 3 4 5 6 7 8 9 10 11 12

A pICH41373 pICH45180 pICH51277 pICH45234 pICH87611 pICH78133 pICSL30008 pICSL50007 pICSL50004 pICH49477 pICSL80016 pICH44300

B pICH41388 pICH45125 pICH51288 pICH45244 pICH41402 pICH78141 pICSL30009 pICSL50009 pICSL50011 pICSL80004 pICSL70002 pICH77901

C pICH45089 pICH45131 pICSL12006 pICH45214 pICH44199 pAGM5331 pICSL30004 pICSL50010 pICSL50015 pICSL80001 pICSL70004 pICH77911

D pICH42211 pICH45152 pICH87633 pICH87655 pICH44222 pAGM1482 pICSL30006 pICSL50012 pICSL50006 pICH75111 pICSL70005 pICH41432

E pICH50581 pICH41551 pICH85281 pICH71292 pICH44233 pAGM5355 pICSL30003 pICSL50013 pICSL80014 pICH42222 pICSL70008 pICH71431

F pICH42760 pICSL13001 pICH88103 pICH71301 pICH44188 pAGM5343 pICSL30010 pICSL50014 pICH41531 pICH43844 pICH41414 pICH71411

G pICH44157 pICSL13002 pICH87644 pICH71311 pICH44179 pAGM1467 pICH37431 pICSL50008 pICSL80005 pICH44022 pICH72400 pICH71421

H pICH45173 pICH51266 pICH45195 pICH71342 pAGM1479 pICSL30005 pICH37326 pICSL50016 pICSL80007 pICSL80012 pICH41421 pAGT707

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How to Cite your Addgene Plasmids in Future Publications (Save for reference)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which they were created, and include Addgene in the Materials and Methods of your future publications.

Information pertinent to your requested plasmids:

Principal Investigator: Nicola Patron Article Reference: A Golden Gate Modular Cloning Toolbox for Plants. Carola Engler, Mark Youles, Ramona Gruetzner, Tim-Martin Ehnert, Stefan Werner, Jonathan D. G. Jones, Nicola J. Patron, and Sylvestre Marillonnet ACS Synth. Biol., Just Accepted Manuscript • DOI: 10.1021/sb4001504 • Publication Date: 05 Feb 2014

Addgene: MoClo Plant Parts Kit

If you have any questions about how to cite these plasmids, please contact Addgene at help@addgene.org or call (617) 225-9000.

Best wishes for many successful publications!