modulation transfer function (mtf)
DESCRIPTION
The resolution and performance of an optical microscope can be characterized by a quantity known as the modulation transfer function (MTF), which is a measurement of the microscope's ability to transfer contrast from the specimen to the intermediate image plane at a specific resolution.TRANSCRIPT
By
AJAL.A.J
MTF
Exposure Systems
Contact Proximity Projection
AJAL.A.J
Introduction: Historical and cultural relevance of MTS
Motivations: Why MTF supported also by incompetents (like me)
Technical issues: How do we achieve MTF and analyse its data (a few examples for MTF)
Scientific rationale: What can we do reasonably and first with MTF ?
SLIDES PREPARED FOR S8 ECE . METS SCHOOL OF ENGINEERING , MALA THRISSUR, KERALA , INDIA ON 10-12-09
modulation transfer function (MTF)
Instrument properties: (1) Linearity
• Linearity tested using high-quality test target
Set of squares of precisely calibrated optical absorbance
Note that the measured absorbance is greater than the real one.
This is due to the extra absorbing elements through which the beam passes between the reference and imaging detectors.
Measured absorbance
Re
al
ab
so
rba
nc
e
Line of unity
Instrument properties: (2) MTF
• MTF measured using alternating bands .
MTF limited by diameter of laser beam.
It is the beam size as it passes through the sample that matters:
Laser scanner design needs to be modified if resolution is to approach that of pixelated (CCD) scanners.
0.5 mm resolution
Instrument properties: (3) SNR
• Experimental test using different dye concentrations
Projection SNR defined by A / (A): direct measurement for squares on HQTT
Note that SNR 0 as A 0
Reconstruction SNR calculated via measurement of uniform dye in matching tank and then using data to simulate a scanned cylinder.
Peak SNR of almost 400 possibility of dose precision to < 0.5% !
Instrument properties: (4) Speed
• To date
Acquisition rate up to 65536 samples / s demonstrated.
Equivalent to a 256 × 256 projection image in 1 s.
Gives 2563 3-D image in 400 s 7 mins. At full Nyquist sampling
Images shown are acquired at around 20 mins. for a 2563 3-D image
This is comparable with the best performance of our CCD scanner
• The future ...
This architecture is very similar to that used in 3-D confocal microscopy.
In that field images are acquired at video frame rates.
We confidently expect 3-D scans in a minute or less.
DEFINE MTF ?
• The resolution and performance of an optical microscope can be characterized by a quantity known as the modulation transfer function (MTF), which is a measurement of the microscope's ability to transfer contrast from the specimen to the intermediate image plane at a specific resolution.
• Computation of the modulation transfer function is a mechanism that is often utilized by optical manufacturers to incorporate resolution and contrast data into a single specification.
• The modulation transfer function (MTF) indicates the ability of an optical system to reproduce (transfer) various levels of detail (spatial frequencies) from the object to the image.
• Its units are the ratio of image contrast over the object contrast as a function of spatial frequency.
• It is the optical contribution to the contrast sensitivity function (CSF).
MTF: Cutoff FrequencyMTF: Cutoff Frequency
0
0.5
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1 mm2 mm4 mm6 mm8 mm
mo
du
lati
on
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nsf
er
spatial frequency (c/deg)
cut-off frequency
57.3cutoff
af
Rule of thumb: cutoff frequency increases by ~30 c/d for each mm increase in pupil size
Another useful concept is the modulation transfer function or MTF, defined as shown below
MTF is the ratio between image intensity modulation over the object intensity modulation
This parameter qualifies the capability of an optical system
Photolithography- MTF
Function describes contrast as a function of size of features on the mask
Generally, MTF needs to be > 0.5 for the resist to resolve features
Photolithography- MTF
Modulation transfer function
i(x, y) o(x,y) PSF(x,y) noise
I(kx ,ky )O(kx , ky )MTF(kx ,ky ) noise
Information Processing by Human Observer
• Visual perception– Concerns how an image is perceived by a human
observer• preliminary processing by eye this lecture• further processing by brains
– Important for developing image fidelity measures• needed for design and evaluate DIP/DVP algorithms &
systems
imageimage eyeeye perceived imageperceived image
understanding of content
The Eye
– Cross section illustrationFigure is from slides at Gonzalez/ Woods DIP book website (Chapter 2)
Two Types of Photoreceptors at Retina
• Rods– Long and thin– Large quantity (~ 100 million)– Provide scotopic vision (i.e., dim light vision or at low illumination)– Only extract luminance information and provide a general overall
picture
• Cones– Short and thick, densely packed in fovea (center of retina)– Much fewer (~ 6.5 million) and less sensitive to light than rods– Provide photopic vision (i.e., bright light vision or at high illumination)
– Help resolve fine details as each cone is connected to its own nerve end– Responsible for color vision
our interest (well-lighted display)
Light
• Light is an electromagnetic wave– with wavelength of 350nm to 780nm stimulating human visual response
• Expressed as spectral energy distribution I()– The range of light intensity levels that human visual system can adapt is
huge: ~ on 10 orders of magnitude (1010) but not simultaneously
Luminance vs. Brightness
• Luminance (or intensity)– Independent of the luminance of surroundings
I(x,y,) -- spatial light distributionV() -- relative luminous efficiency func. of visual system ~ bell shape
(different for scotopic vs. photopic vision; highest for green wavelength, second for red, and least for blue )
• Brightness– Perceived luminance– Depends on surrounding luminance
Same lum. Different brightness
Different lum.
Similar brightness
Luminance vs. Brightness
• Example: visible digital watermark– How to make the watermark
appears the same gray levelall over the image?
Testing MethodologyTesting Methodology
Study Objective• To compare the image quality of different designs using
the modulation transfer function (MTF) testing method
Modulation Transfer Function (MTF) Testing• Objective method of measuring image contrast
degradation at different spatial frequencies
Study Objective• To compare the image quality of different designs using
the modulation transfer function (MTF) testing method
Modulation Transfer Function (MTF) Testing• Objective method of measuring image contrast
degradation at different spatial frequencies
The optical bench setup
Pinhole Target
IOL
Light Source
CCD Camera
MTF measurement systemMTF measurement system MTF measurement systemMTF measurement system
paradigmsparadigms
The IQ ReSTORThe IQ ReSTOR®® SN6AD3 aspheric SN6AD3 aspheric
intraocular lensintraocular lens (IOL) (IOL) produced the highest produced the highest overall image quality for MTFoverall image quality for MTF
Clinical investigation is needed to determine whether Clinical investigation is needed to determine whether superior IOL optical quality demonstrated in optical superior IOL optical quality demonstrated in optical bench testing results in measurable visual bench testing results in measurable visual improvements in clinical practiceimprovements in clinical practice
The IQ ReSTORThe IQ ReSTOR®® SN6AD3 aspheric SN6AD3 aspheric
intraocular lensintraocular lens (IOL) (IOL) produced the highest produced the highest overall image quality for MTFoverall image quality for MTF
Clinical investigation is needed to determine whether Clinical investigation is needed to determine whether superior IOL optical quality demonstrated in optical superior IOL optical quality demonstrated in optical bench testing results in measurable visual bench testing results in measurable visual improvements in clinical practiceimprovements in clinical practice
Image quality
• Spatial resolution can be best described by modulation transfer function (MTF)
• The limiting resolution of an imaging system is where the MTF approaches zero
• Higher magnification modes (smaller fields of view) are capable of better resolution
• Video imaging system degrades the MTF substantially
Color of LightColor of Light
Perceived color depends on spectral content (wavelength composition)
– e.g., 700nm ~ red.– “spectral color”
A light with very narrow bandwidth
A light with equal energy in all visible bands appears white
“Spectrum” from http://www.physics.sfasu.edu/astro/color.html
Perceptual Attributes of Color Perceptual Attributes of Color
Value of Brightness (perceived luminance)
Chrominance– Hue
specify color tone (redness, greenness, etc.)
depend on peak wavelength
– Saturation describe how pure the color is depend on the spread
(bandwidth) of light spectrum reflect how much white light is
added
RGB HSV Conversion ~ nonlinear
HSV circular cone is from online documentation of Matlab image processing toolbox
http://www.mathworks.com/access/helpdesk/help/toolbox/images/color10.shtml
• Any color can be reproduced by mixing an appropriate set of three primary colors
(Thomas Young, 1802)
Representation by Three Primary Colors
Example: Seeing Yellow Without Yellow
mix green and red light to obtain perception of yellow, without shining a single yellow photon
520nm 630nm570nm
=
Fourier Transform
The co-ordinate (ω) in Fourier space is often referred to as spatial frequency or just frequency
Graphical Representation Of The Fourier Transform
Convolution
Transfer Functions
• In Fourier Space this representation is simplified
)()()( sTsXsX inputoutput
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x =
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Point Spread Function (PSF)
• The blurring of an imaginary point as it passes through an optical system
• Convolution of the input function with a
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dudvvuh
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YX
YX 2
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),(
)](2exp[),(),(
H
By international agreement, the function is known as the optical transfer function (OTF) of the system and it is also the normalized autocorrelation of the amplitude transfer function.
Its modulus H is known as the modulation transfer function (MTF).
)(
)(PTFieMTFOTF
Where PTF is phase transfer function.
• MTF is the Fourier Transform Of the PSF
• MTF is a Transfer Function
CONCLUSION
)(
)(PTFieMTFOTF
Modulation Transfer Function (MTF)
• A representation of the point spread function in Fourier space
0
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x =
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Transfer functions:
Optical transfer function T (OTF)
Modulation transfer function M (MTF)
Phase transfer function (PTF)
Frequency convolution theorem:
}{}{2
1}{ hFfFhfF
Example: Transform of a Gaussian wave packet.
akkxikax ekFeea
xE 4/)( 200
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)( ,)(
Please prove it.
MTF Definition
• MTF is a measure of intensity contrast transfer per unit resolution of an image or signal.
• It is used in optics, electronics, and related signal processing applications.
Imaging TaskAs spatial separation decreases, the “good” system maintains clear separation of point source images, while the “poor” system eventually can no longer distinguish them.
MTF quantifies this phenomenon in terms of contrast between the center peak intensities versus intensity at their midpoint across a scale of separation distances.
At large separations, even a poor system can completely resolve the two images. As separation decreases, only the good systems can still recognize separate sources.
Good Poor
Contrast Modulation: A Basic MTF
Contrast Modulation is defined simply by averaging the difference of maximum and
minimum transmitted intensities:
“Spatial Frequency” typically implies an array of sine or bar targets at a given spacing, expressed in line-pairs-per-millimeter (lp/mm)
or cylces -per-milliradian (cy/mrad)
ContrastI I
I I
max min
max min
0 200 400 600 800 10000
0.1
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1
One line pair
Imax
Imin
Original Signal
Output Signal
Nyquist Sampling TheoryNyquist Theory: In order to achieve perfect reconstruction of an input signal which has a maximum spatial frequency "f" (the cutoff), sampling must occur at a rate of at least "2f". (Note: Phase is still an issue!)
Sample Intervals in Phase
Inpu
t Wav
efor
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ampl
ed O
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t
Samples Out of Phase
Inpu
t Wav
efor
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Optical MTFImaging optical systems perform sampling, with the maximum sample frequency determined by the “spot size” image of a perfect point source object (e.g., “Impulse Response”).
A “perfect” optical system is limited in resolution by wavelength dependent diffraction effects. Lens aberrations can only worsen performance.
The MTF of an optical system is found by Fourier operations on the “spot size”, or Point Spread Function.
ImageObject
System MTFThe MTF of cascaded optical assemblies is NOT equal to the product of component MTF’s!
Why? Lenses transmit not just intensity, but wavefront phase as well, and hence aberrations in one lens can cancel those in another.
MTF of cascaded objective lenses, detector, and displays may be multiplied for composite “System MTF”, with a component MTF measured at each intensity transfer point.
Focal Plane Image Focal Plane Image
MTFsystem = MTFobjective*MTFdetector*MTFdisplay*MTFeyepiece*MTFeye
MTFsystem MTFLens1*MTFLens2
System MTFExample System MTF calculation:
Freq. ObjLens FPA Display Eyepiece System
cy/mrad MTF MTF MTF MTF MTF
1.0 0.9999 0.9999 0.9999 0.9999 0.99
2.0 0.90 0.99 0.99 0.90 0.79
3.0 0.85 0.98 0.92 0.88 0.67
For average human observers, MTF values around 0.05 are considered barely resolvable. If the above system MTF reached 0.05 at 10 cy/mrad, for example, then you can predict that a human observer could identify (6 cylce criteria) a 2.4 meter taget through this sensor at a maximum range (this is a coarse estimate!) of about
Range mm cycles
mradkm( )
[2. ] [6 ]
tan[ / ]
4
1 104
Measurement: Knife EdgeKnife Edge Measure of the Line Spread Function (LSF):
1. Drag a knife-edge across the focal plane of the optic to be tested and record the intensity
2. Calculating the derivative of this data gives us the LSF we are looking for so we can continue with MTF.
Edge Response
Line Spread
Differe-ntiate
Measurements of Image Quality
• PSF = Point Spread Function
• LSF = Line Spread Function
• CTF = Contrast Transfer Function
• MTF = Modulation Traffic Function
Point Spread FunctionPSF
• “Point” object imaged as circle due to blurring
• Causes– finite focal spot size– finite detector size– finite matrix size– Finite separation between object and detector
• Ideally zero
– Finite distance to focal spot• Ideally infinite
Quantifying Blurring• Object point becomes image circle
• Difficult to quantify total image circle size– difficult to identify beginning & end of object
Intensity
?
Quantifying BlurringFull Width at Half Maximum (FWHM)
• width of point spread function at half its maximum value
• Maximum value easy to identify
• Half maximum value easy to identify
• Easy to quantify width at half maximum
FWHM
Maximum
HalfMaximum
Line Spread FunctionLSF
• Line object image blurred
• Image width larger than object width
Intensity
?
Contrast Response FunctionCTF or CRF
• Measures contrast response of imaging system as function of spatial frequency
Lower
Frequency
Higher
Frequency
Loss of contrast between light and dark areas as bars & spaces get narrower. Bars & spaces blur into one another.
Contrast Response FunctionCTF or CRF
• Blurring causes loss of contrast– darks get lighter– lights get darker
Lower
Frequency
Higher
Frequency
Higher
Contrast
Lower
Contrast
Modulation Transfer FunctionMTF
• Fraction of contrast reproduced as a function of frequency
Recorded
Contrast(reduced by blur)
frequency
MTF
1
0
Contrast provided
to film
Freq. =
line pairs / cm
50%
MTF
• Can be derived from– point spread function– line spread function
• MTF = 1 means– all contrast reproduced at this frequency
• MTF = 0 means– no contrast reproduced at this frequency
MTF• If MTF = 1
– all contrast reproduced at this frequency
Recorded
Contrast
Contrast provided
to film
MTF• If MTF = 0.5
– half of contrast reproduced at this frequency
Recorded
Contrast
Contrast provided
to film
MTF• If MTF = 0
– no contrast reproduced at this frequency
Recorded
Contrast
Contrast provided
to film
Modulation Transfer Function (MTF)
MTF = Imax- Imin
Imax + Imin
-MTF is a measure of the contrast of an aerial pattern,
-For well-separated images,MTF ~ 1,
-For smaller images, MTF<1
-In general, MTF should be >0.5.
IntensityIntensity
DisplacementDisplacement
Component MTF• Each component in an imaging system has its own
MTF– each component retains a fraction of contrast as function
of frequency
• System MTF is product of MTF’s for each component.
• Since MTF is between 0 and 1,
• composite MTF <= MTF of poorest component
Modulation Transfer Function
• MTF is a measure of an imaging system’s ability to recreate the spatial frequency content of scene
MTF is the magnitude of the Fourier Transform ofthe Point Spread Function / LineSpread Function.
1.0
Cut-off
Spatial frequency
3 steps for MTF Measurement of IJ Printer
MTF of an IJ printer
1. Print a test target page 2. Scan the printed target
3. Analyze scanned data
Test Target Page
MTS : for noise reduction example
Low frequency noise artifacts are visible in the sky, which has been enhanced (darkened and boosted in contrast) for aesthetic purposes.
This is clear evidence of noise reduction– in a camera has an excellent reputation for low noise. The CMOS sensor evidently requires noise reduction.
MTF measurement methods: 1] Point source METHOD
Xe lamp: 3kW Xe lamp: 1kW
MTF measurement methods: step edge
Step edge method– Image of a target (artificial or natural) with a sharp transition between
dark and bright area– With a slight edge inclination, we can interleave successive rows (or
columns) to rebuild a sufficiently sampled response to Heaviside function
• Again, this is not necessary with THR mode
– Modulus of ratio of FT (edge response) to FT (edge) = in-flight MTF
Two kinds of edge– Natural edge: agricultural fields
• Difficulty to find a good one and to validate it
– Artificial edge• A checkerboard target has been laid out (Salon-de-Provence in south of France)• 60 x 60 m
Method Description• Edge Method (MTF estimation method)
– Sub-pixel edge locations were found by Fermi function fit.
– A least-square error line was calculated through the edge locations.
– Savitzky-Golay Helder-Choi filtering was applied on each line
– The filtered profile was differentiated to obtain LSF
– MTF calculated by applying Fourier transform to LSF.
Fig 1. Edge Method
• Pulse method– A pulse input is given to an imaging system.– Output of the system is the resulting image.– Edge detection and SGHC filtering was applied to get
output profile. – Take Fourier transform of the input and output.– MTF is calculated by dividing output by input.
Figure 2. Pulse method
10 12 14 16 18 20 22 24
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Edge detection
Pix
els
Pixels
Curve inflection pointLeast square fit line
MTF measurement methods: Bi-resolution
Principle– Same landscape acquired with two spatial resolutions (same
spectral band)• High resolution image = reference• Low resolution image = sensor under assessment
– In-flight MTF = Modulus of ratio of FT (LR image) to FT (HR image)
Two situations– Satellite image versus aerial image
• Attempt with SPOT4 HRVIR
– Both sensors on the same satellite• Attempt with SPOT4: VGT1 versus HRVIR
MTF measurement methods: Periodic target
Opportunity to acquire Center radial target
THRESHOLD (5m) THRESHOLD (2.5m)
APPLICATIONSAPPLICATIONSThe MTF curves are used to characterize photographic The MTF curves are used to characterize photographic
objectives and are determined by the comparison of input objectives and are determined by the comparison of input images with their photographic reproductions. images with their photographic reproductions.
With this study the procedure is also extended to cases With this study the procedure is also extended to cases where the characterization of the acquisition system is not where the characterization of the acquisition system is not
possible.possible.
?ACQUISITION SYSTEM
OUTPUT IMAGE
INPUT IMAGE
?
MTF(f
f
ACQUISITION SYSTEM
OUTPUT IMAGE
INPUT IMAGE
MTF(f
f
AIM: AIM: extension of the traditional MTF technique to evaluate the extension of the traditional MTF technique to evaluate the debated resolution of the body image of the Turin Shroud. debated resolution of the body image of the Turin Shroud.
"“Relic certainly it is…”"“Relic certainly it is…” (John Paul II, April 28(John Paul II, April 28thth 1989) 1989)““The Shroud is provocation to the intelligence … The Shroud is provocation to the intelligence …
The Church submits to the scientists the assignment toThe Church submits to the scientists the assignment to keep on investigating”keep on investigating” (John Paul II, Turin 1998(John Paul II, Turin 1998
Purdue UniversityHP-Purdue Confidential 77
MTF (Modulation Transfer Function)MTF (Modulation Transfer Function)
• The MTF of an imaging system The MTF is the magnitude of the OTF (optical transfer function) as
.)0(
)()()(
H
fHfOTFfMTF
If the input signal x(·) is an impulse, then the MTF can be obtained by calculating |Y(f)| / |Y(0)|, where Y(f) is the impulse response of the system.
The MTF describes how much the system attenuates the input modulation signal as a function of frequency.
• MTF measurement by using a set of sinusoidal signals Measure the output response for a set of sinusoidal signals.
Extract the output magnitude at the given frequency for each sinusoidal signal.
Normalize the extracted output magnitudes and estimate the MTF curve.
Questions & Contacts
AJAL.A.J --- [email protected]
Phone: (9633 – 910 911)
www.ajal4u.0catch.com
http://www.metsengg.org/dept_home.php?dept_name=ece&opt=faculty
References• Modulation Transfer Function (MTF)
– Implement and compare different methods for calculating/measuring the MTF of an imaging lens
– Reference:• Backmann et al., “Random target method for fast MTF inspection,”
Optics Express, vol. 12, no. 12, pp. 2610 (2004)
• MTF with defocus– Implement and evaluate the MTF for a defocusing error– Reference:
• C. S. Williams, O.A. Becklund, “Introduction to the Optical Transfer Function,” (1989)
Thanks so much. It has been a pleasure.
Enjoy the meeting!
AJAL.AJ, ME .Assistant ProfessorMETS School of EngineeringUniversity of CalicutMala , Thrissur ( Dt )Email: [email protected]