molecular axon guidance
DESCRIPTION
Molecular Axon Guidance. PC12 cell…. then NGF added. NGF is neurotropic and neurotrophic. Guidance Factors. Growth Factors. Netrin..was one of the first neurotropic factors. Findings. Netrin from FP. Netrin from FP, But perpendicular. Netrin in media. Cell locomotion like GCs. - PowerPoint PPT PresentationTRANSCRIPT
Molecular Axon Guidance
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PC12 cell….then NGF added
NGF is neurotropic and neurotrophic
Growth FactorsGuidance Factors
Netrin..was one of the first neurotropic factors
Findings
Netrin from FP
Netrin from FP,But perpendicular
Netrin in media
Cell locomotion like GCs
Cell moves forward by extending filipodia & lamellipodiaFocal adhesions are formedCell is pulled forward
Diffusible Contact dependent
Extracellular matrix
Extracellular matrix
Proteins of ECM - collagen
Extracellular matrix
Proteins of ECM - proteoglycans
Extracellular matrix
Proteins of ECM -
fibronectin
Extracellular matrix
Proteins of ECM - laminin
collagen
laminin
Interaction of cells with ECM
inactive
INTEGRINS
Interaction of cells with ECM
INTEGRINS
functions of outside-in signals
differentiationmotilitygrowth survival
ligands have RGD
motif
Interaction of cells with ECM
FOCAL ADHESIONS
FA
Interaction of cells with ECM
FOCAL ADHESION
S
Interaction of cells with cells
CELL ADHESION MOLECULES
Interaction of cells with ECM and other
cells
CELL ADHESION MOLECULES VS
ECM ATTACHMENT MOLECULES
Tcf/Lef
PE-Cadherin
-catenin p120
P
Wnt
Dsh
Frz II
ExtracellularSpace
actinfilaments
-catenin
Ca 2+
Gsk3
APCAxin
• Focus is often on adhesive function: Cadherins as molecular glue
Cadherins as Master Regulators of Morphology
Cadherins
Actin Cytoskeleton
-cateninRho-GTPasesArp2/3
Proliferation
CDKI: p27/p21Wnt
Motility
Passive InhibitionRho-GTPasesActive Promoter
Adhesion
AJ’sType of CadherinStabilize others junctions
ReceptorSignaling
EGFFGFPDGF
Transcriptional/Nuclear Role
-catenin/WntP120/kaisoIn nucleus