molecular biology techniques (elisa)
TRANSCRIPT
INTRODUCTION
ELISA is a trustworthy mehtod for antigen or antibody presence
evaluation.
ELISA Constitutes an evidence for immunological interactions
SEROLOGICAL EVALUATION OF IMMUNITY TO THE
VARICELLA ZOSTER VIRUS BASED ON A NOVEL
COMPETITIVEFebruary 6, 2016.
1. Detection mode: Addition of antibodies to ELISA plates to capture recombinant virus protein
2. Use of labelled neutralized mAbs to block serum antibodies
3. Analysis. Blocking rate indicates neutralizing antibody on serum
I think it is a great begininig because the results of these techniques will allow the development of strategies that improve treatment of viral infections, immunization ways and identification of most expressed virus proteins and which antibodies are responsible to protect us against patogens.
Thanks to the studies realized was established a
new alternative method that open the door for new
investigations about the interactions between
monoclonal antibodies and virus proteins
STUDENT´S OBSERVATION
IMMUNODIAGNOSIS OF FASCIOLA GIGANTICA INFECTION USING
MONOCLONAL ANTIBODY-BASED SANDWICH ELISA AND
IMMUNOCHROMATOGRAPHIC ASSAY FOR DETECTION OF CIRCULATING
CATHEPSIN L1 PROTEASE
Faculty of science, Mahidol UniversityJanuary 1, 2016.
Parasitological diagnosis of fasciolosis is possesses low sensitivity.
Detection of circulating parasite antigens is thought to be a better alternative for
diagnosis of fasciolosis.
The study was realized in cattle and mice infected with
fasciola gigantica serum.
MoAb 4E3 and biotinylated rabbit anti-recombinant CatL1
antibody were used due to their high specificities.
I think that is interesting that despite of the immense quantity of efficient methods for diagnosis and treatment of different kind of diseases, scientists are constantly searching to improve it. This investigation have allowed an efficacy increment for diagnosis of an infection caused by a parasite, constituting the first step to begin its correct and fast treatment.
Main purpose of both investigations was to validate an accurate method to establish the immunity state of an
individual.
The Implementation of ELISA method
allows the identification of
presence of antigens in patients and the
establishment of how is functioning their immune system in
response of thereof.
I think this method can be used for epidemiological investigations and the results of these studies at the same time will contribute to the development of alternatives for
primary prevention such as better immunization programs.
BIBLIOGRAPHY Jian Liu & Xiangzhong Ye. (2016). Serological Evaluation of Immunity to the Varicella-
Zoster Virus Based on a Novel Competitive Enzyme-Linked Immunosorbent Assay. Febreo 28. 2016, de scientific reports Sitio web: http://www.nature.com/articles/srep20577#abstract
Anuracpreeda P, Chawengkirttikul R, Sobhon P. (2016). MEDLINE® Immunodiagnosis of Fasciola gigantica Infection Using Monoclonal Antibody-Based Sandwich ELISA and Immunochromatographic Assay for Detection of Circulating Cathepsin L1 Protease.. March 1, de Medline Sitio web: https://www-clinicalkey-es.consultaremota.upb.edu.co/#!/content/medline/2-s2.0-26731402
MARTINEZ SÁNCHEZ, Lina María. Biología molecular. 8va ed. Medellín: UPB. Fac. de
Medicina, 2015. 200,201 p.