MOLECULAR DIAGNOSTICS CORE

HOUSTON METHODISTRESEARCH INSTITUTE

Molecular Diagnostics Core

Core Director: Randall J. Olsen, MD, PhD713.441.6802Email: rjolsen@houstonmethodist.org

Core Staff: Dilzi Mody713.441.1771 Email: dfmody@houstonmethodist.org

houstonmethodist.org/molecular-diagnostics

Houston Methodist Research Institute

6670 Bertner Avenue

Houston, TX 77030

houstonmethodist.org/research

HMRI Communications & External Relations | RICORE-018 | MM | DH | 250 | 08.2014

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HeartInstitute

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MemorialHermannHospital

Garage1 LA

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FLEM

ING A

VE.

Ben TaubGeneralHospital

BaylorCollege

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Molecular Diagnostics Core

The Houston Methodist Research Institute Molecular Diagnostics Core facility provides Houston Methodist investigators and the Texas Medical Center community with access to advanced molecular tools for research studies.

All equipment is conveniently located in the Molecular Diagnostics Core Laboratory in Fondren 554. User training and instrument reservations can be scheduled using iLab.

FACILITY

The Molecular Diagnostics Core provides investigators access to cutting-edge instrumentation for expression analysis, SNP genotyping, and other molecular assays. Some of the available technologies include:

Capillary Electrophoresis – The Agilent 2100 is a microfluidics-based platform offering solutions for the analysis of DNA, RNA, proteins and cells. Typical applications include: • RNA sample QC prior to microarray or

qPCR experiments • DNA Analysis of PCR/mPCR and RT-PCR

products • Protein expression analysis • Protein purity during purification • Cell-based analysis in gene silencing or

apoptosis

Spectrophotometry – The NanoDrop® ND-1000 UV-Vis Spectrophotometer enables highly accurate analyses with remarkable reproducibility, eliminates the need for cuvettes and capillaries, and need for most dilutions. This technology is used for: • Nucleic Acid Measurement • Microarray labeling QC • Protein Measurement • Cell Culture

Microarray Instrumentation – The Affymetrix GeneChip® Instrument System is one of the most advanced microarray analysis platforms available to generate reliable, Gene Expression Analysis and DNA Analysis data. The Dell Precision 490 workstation accompanies the GeneChip System.

Software • Affymetrix Software • Partek Genomics Suite

The core offers training for the use of instrumentation, assistance in the design of experimental protocols, and ongoing consultation and support. Core instrumentation is available for self-use by trained investigators.

OurSpecializedServices

THE GENECHIP® INSTRUMENT SYSTEM