molecular pathology catalog 2014

®With a family of Xmatrx systems, you have the freedom to

attend to more demanding tasks while delivering high-quality

and standardized results every time

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www.biogenex.com

In the U.S., call +1 (800) 421-4149

Outside the U.S., call +91-40-27185500

© 2014 BioGenex Laboratories, Inc. All Rights Reserved

For distribution worldwide except USA and CanadaISO 13485:2003

FM 78972

*Optional Software for Research Use Only

Doc. No. 937-4083.0

(International)

Three Simple Steps

Load Click View

Molecular Pathology Catalog

2014 - 2015

Microtome to Microscope

*IHC | Special Stains | FISH & ISH | miRNA | Multiplex IHC & ISH | in situ PCR

All-in-One All-at-Once • The World’s First and Only Fully Automated

Front-end FISH Processing System

• Run up to 40 slides under multiple protocols

• Reduce hands-on tech time from 7.5 hours to 30 minutes

T

• T

• R

• R

[email protected]

Dear Customer,

We are pleased to present the BioGenex Molecular Pathology Catalog for 2014 - 2015. As a vertically integrated company we develop, manufacture and market highly innovative and fully automated systems for cancer diagnosis, prognosis and therapy selection.

Xmatrx® systems redefine complete automation for the molecular pathology laboratory and standardize all the steps from baking through final cover-slipping in three simple steps - Load, Click and View. Compared to any other system on the market, Xmatrx® systems offer clean intense stain(s), automate more assay steps, and enable automation of technologies for the future molecular pathology laboratory.

• Xmatrx® ELITE integrates All-in-One and All-at-Once staining of IHC, ISH, special stains and beyond • Xmatrx® Infinity is a high-performance staining platform for life sciences and translational research • Xmatrx® ULTRA is the next-generation system with new features such as touch screen and SMS intelligence • Xmatrx® NANO is a ten-slide automated system specifically designed for FISH • Xmatrx® MINI enables in situ PCR and nucleic acid hybridization with tools for building micro-chamber

We also offer a series of i6000TM systems with very high throughput; 200 slides in an 8-hour shift.

To maintain our tradition of offering superior solutions for the emerging needs of your laboratory, we offer a broad range of molecular pathology products for IHC, ISH, miRNA, multiplex and special staining of tissues including 300+ primary antibodies, molecular probes, detection systems, and ancillaries. These are offered for standardized, reliable and consistent results to support the needs of molecular pathology laboratories of today, tomorrow and beyond.

BioGenex is committed to the core values of innovation, reliability, productivity, quality and superior after sales support and service for complete customer satisfaction. These values are represented by our company’s colors that stand for “energy and innovation” (orange) and “reliability” (blue).

I invite you to learn more about our exciting products and future development through this catalog and our new website at www.biogenex.com. Should you have any suggestions for improving our products and services, I encourage you to write me directly at [email protected].

Give us an opportunity and experience the difference.

Warm Regards,Krishan Kalra, Ph.D.CEO

iiwww.biogenex.com

Dr. Krishan Kalra

Innovation

Quality

Service

Reliability

Productivity

To become a global

molecular medicine company

providing affordable solutions

for life science research and

personalized medicine

“

“

[email protected]

Table of Contents

Overview.....................................................................................iv

General Information..................................................................v

Additional Information .............................................................v

Ordering Information.................................................................vi

Technical Information...............................................................vi

Automated Systems Xmatrx® ELITE...........................................................................02

Xmatrx® ULTRA.........................................................................03

Xmatrx® NANO..........................................................................04

Xmatrx® MINI............................................................................05

Xmatrx® Infinity.........................................................................06

Xmatrx® FISH (eFISHiency)......................................................07

i6000TM Diagnostics................................................................08

i6000TM Infinity........................................................................09

i500 PlusTM...............................................................................10

Xmatrx® Associated Reagents and Consumables.................11

i6000TM Associated Reagents and Consumables.................23

i500 PlusTM Associated Consumables....................................30

Tissue Pre-treatment & Antigen RetrievalEZ-DeWaxTM Solution................................................................32

Antigen Retrieval Method........................................................33

Antigen Retrieval Accessory Kit..............................................34

EZ-ARTM Elegance Solutions....................................................34

EZ-ARTM Solutions....................................................................35

Antigen Retrieval Solutions.....................................................36

Enzymes for Tissue Digestion.................................................38

NordicWare® Microwave Tender Cooker.................................39

Primary AntibodiesAntibody Look-Up Table.......................................................... 43

Antibody Details.......................................................................51

IHC Detection SystemsSuper SensitiveTM IHC Detection Systems...........................138

Super SensitiveTM One-Step Polymer-HRP Detection-

Systems.................................................................................139

Super SensitiveTM Link-Label IHC Detection Systems.........140

Links and Labels...................................................................142

Multi-StainingSuper Sensitive™ Double Stain Polymer Detection System.......144

Super Sensitive™ Antibody Cocktails......................................145

ISH Probes & ISH Detection Systemsin situ Hybridization Probes.....................................................150

microRNA Probes......................................................................152

Hybridization Detection System...............................................158

eFISHiencyeFISHiency................................................................................160

eFISHiency probes....................................................................161

eFISH pretreatment kits...........................................................163

eFISH Processing systems.......................................................164

Special StainsSpecial Stains...........................................................................168

Ancillary ReagentsBuffers and Diluents................................................................176

Blocking Reagents....................................................................177

Substrates and Chromogens...................................................178

Negative Control Sera/Immunoglobulins...............................179

Counterstains and Mounting Media.......................................180

Microscope Slides and Accessories........................................181

Tissue ControlPositive Control Slides and Barrier Slides..............................184

IndexAlphabetical Product Name Index .........................................194

Catalog Number Index.............................................................210

Antibody Clone Index...............................................................219

Listing by Categories...............................................................228

General Terms and Conditions...............................................237

For latest product offerings visit our website www.biogenex.com or contact our customer support: [email protected]

ivwww.biogenex.com

Overview

BioGenex celebrated its 33rd anniversary serving the anatomic pathology market. We take great pride in providing premier service and support while bringing new and technologically advanced products to the market.

BioGenex provides a “Total Solution” for slide-based cell and tissue analysis. Our products include a wide variety of antibodies, highly sensitive detection kits, and probes for ISH. Our automated systems streamline operations in molecular and cellular pathology laboratories, providing effective tools for the detection and diagnosis of cancer and other diseases. BioGenex continues to innovate as evidenced by the launch of the Xmatrx®

Staining System which provides complete automation “From Microtome to Microscope”.

We are committed to providing our customers and our distributors with flexible, innovative and cost-effective tools for clinical diagnostics, life science research and drug discovery.

Service We value you and your business. We want our relationship to be one of total satisfaction. Our Technical Support Specialists provide fast troubleshooting advice and technical information and they are responsive to your individual needs. Just visit our website at www.biogenex.com, send an e-mail to [email protected] or call toll free at 1-(800)-421-4149 from 7:00 AM to 4:00 PM (PST), Monday through Friday, with your request.

Quality BioGenex is committed to excellence by providing high-quality products. We offer a broad range of products which are manufactured using state-of-the-art equipment in controlled environments. They are stringently tested to ensure that they meet or exceed functional, dimensional, and environmental requirements and are compliant with federal regulations. Our automated systems are designed for high-throughput at a low cost of ownership. They provide consistent quality results with ease of use and maximum flexibility for clinical diagnostics, life science research, and drug discovery markets.

Reliability BioGenex products give consistent, reproducible and reliable results. Our automated systems are highly reliable and dependable, giving our customer peace of mind.

Innovation BioGenex has a rich history of innovation in the field of Immunohistochemistry (IHC) and In situ Hybridization (ISH). BioGenex has a strong intellectual portfolio, consisting of several US and foreign-issued patents, in the areas of

• DNA labeling and amplification • Antigen retrieval and deparaffinization • Automation of tissue and cell sample preparation • Automated IHC, and staining of nucleic Acids • Antigen retrieval and nucleic acid retrieval for tissues

Productivity BioGenex has automated cell and tissue analysis to accelerate clinical diagnostics and drug discovery development. We have developed the total walk-away, industrial scale automated systems to streamline and standardize an array of processes for cell and tissue testing in IHC, ISH/CISH, FISH, and image analysis applications. We offer a “Total Solution” automating every aspect of the histology slide preparation “From Microtome to Microscope”. These technologies significantly increase laboratory operation productivity for clinical diagnostics, drug discovery and life sciences research applications by providing high-quality staining and imaging solutions.

[email protected]

Web Site For the latest information on new product releases, special offers and for placing an online quote request, please visit our new website, www.biogenex.com

Customer SupportOur technical support and customer service specialists are ready to provide fast and detailed Information for your questions and needs. Please call our toll-free number to reach us.

Technical Support Tel: 1-(800)-421-4149 (Option 2) Fax: 1-(510)-824-1490 E-mail: [email protected] Website: www.biogenex.com Customer ServiceTel: 1-(800)-421-4149 (Option 1) Fax: 1-(510)-824-1490 Fax: 1-(888)-866-2500 (Orders Only)E-mail: [email protected]

Corporate OfficeBioGenex Laboratories, Inc. 49026 Milmont Drive Fremont, CA 94538 Tel: 1-(510)-824-1400 Fax: 1-(510)-824-1490

Corporate Business For general business matters not related to product orders or inquiries, please call us at 1-(510) 824-1400 or fax your correspondence to our main corporate business fax: 1-(510) 824-1490.

TrademarksThe following are trademarks of BioGenex Laboratories, Inc. USA

BioGenex® EZ-ARTM

EZ-Retriever® MultiLink®

Super SensitiveTM i6000TM

EZ-DeWaxTM GenoMx®

i500 PlusTM Xmatrx®

Power BlockTM XMountTM

OptiPlusTM XVizTM

InSite® Super Mount®

XWashTM XISHTM

Nationwide Training WorkshopsAs a service to our customers, BioGenex has developed lectures and workshops on the full range of Immunohistochemistry and in situ Hybridization techniques. Please call our Technical Support Department or Regional Account Executive for more information on how you can participate in our educational workshops. Topics include the following:

• Basic Immunohistochemistry • Cancer Panels • Microwave-Based Antigen Retrieval • ER/PR Immunostaining • Troubleshooting • Automation • in situ Hybridization • Double Staining • Multiplexing and Co-detection of Protein and Nucleic Acid Biomarkers

Free Technical LiteratureIn addition to the educational brochures produced by BioGenex, we offer other technically useful information to the histopathology specialists on our website, www.biogenex.com where you can download our data sheet, product catalog or relevant presentation that may accompany each product assay protocols, kit instruction manuals and conference posters. Please call our Technical support department to request specific items or to add your name to our mailing list.

Technology Partnering OpportunitiesWe are always interested in licensing innovative technology that will be useful to our customers. If you are a researcher and have new antibody clones or other new diagnostic technologies please think of BioGenex as a potential partner in marketing your inventions and discoveries. We have the scientific expertise and marketing experience necessary for the successful commercialization of your technical achievements. BioGenex has an active Research and Development program fully staffed with PhD and MD professionals who are experienced in immunopathology, protein chemistry, and molecular biology. For more information on technology transfer opportunities, please contact us at [email protected]

Additional InformationGeneral Information

Upgraded

viwww.biogenex.com

Technical InformationAll BioGenex products have been listed in this catalog under easily identifiable product groups. The products have also been indexed at end of the catalog under the following headings:

• Alphabetical Product Name Index • Catalog Number Index • Antibody Clone Index • Listing By Categories

Symbol keys used in different sections have been defined on the same page for quick and easy reference. The BioGenex Molecular Pathology Catalog 2014 - 2015 is also available on our website, www.biogenex.com

Ordering InformationInternational Orders To place an order from outside the US, please contact your local BioGenex channel partner/distributor. Please visit our website www.biogenex.com, for more details. For countries where BioGenex does not have any channel partners/distributors, please e-mail us at [email protected] Payment All payments must be made in U.S. dollars. The following methods of payment are accepted:

• Bank transfer (see Proforma Invoice for instructions) • Check, drawn on a U.S. bank, made payable to:

“BioGenex Laboratories, Inc.”

Automation

2

Automated Systems

www.biogenex.com


* optional software

Fully Automated System from Microtome to Microscope...For the Molecular Pathology Laboratory of Today, Tomorrow and Beyond

• All-in-One All-at-Once – IHC, ISH, SS, Multiplexing and Co-detection

• FISH*

• Standardized process from baking through final glass cover slip in three simple steps: Load – Click – View slides

• Automates technologies of today…IHC, ISH, SS, FISH*, Multiplexing and Co-detection of Nucleic Acid and Protein Biomarkers

• Designed to automate the technologies of tomorrow and beyond…Gene Expression Profiling, in situ PCR, miRNA… and more

• Meets global regulatory requirements and safety standards

3

Automated Systems

[email protected]

Next Generation Fully Automated Staining System

• Next generation fully-automated slide staining system

• BioGenex’s proprietary coverslip mechanism

• Easy waste disposal system

• Intelligent SMS system

• Auto-DAB enabled – On-board automated mixing of chromogen and buffer

• High throughput - 100 slides per day, 60 slides in eight-hour shift, and 40 slides in delayed overnight run

All-in-One All-at-Once - IHC, ISH, SS, Multiplex and Co-detection

4

Automated Systems

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eFISHiency System for FISH Automation

• Add micro-reagents manually to save cost

• Intelligent SMS information for alerts

• Economical and affordable

• Touch Panel PC as user interface

• Flexible Open System Software - create, edit and save protocols for future use

• Run 10 different protocols at the same time

All-in-One - FISH, in situ PCR and ISH

5

Automated Systems

[email protected]

• High performance in situ PCR and FISH

• Hybridizer with eXACT™ temperatures

• 10 independent thermal cyclers

• Built-in touch screen display for easy operations

• Facility of on-board wash with effective waste drainage system

• Audio-visual alerts and on screen color-coded error alerts

• User-friendly software with ability to add/delete cycles, store protocols for future use and perform up to 45 PCR cycles

eFISHiency Workstation

All-in-One - FISH, in situ PCR and ISH

6

Automated Systems

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Infinite Possibilities... ...For Translational and Clinical Research

• Intelligent and flexible system offering infinite possibilities – IHC, ISH, FISH, CISH, IF, Multiplexing and Co-detection

• Simultaneous optimization of up to 40 parameters in single run

• Reaction micro-chamber reduces micro-reagent consumption by up to 90%

• 40 independent thermocyclable (PCR) workstations

• Intuitive software designed for ease of use and flexibility

• Reports for inventory management and regulatory compliance

• Multiple slide processing options – Random, Continuous and STAT

All-in-One - IHC, IF, ISH, CISH, FISH, in situ PCR and miRNA...

7

Automated Systems

[email protected]

Integrated Workflow Solutions for Optimizing Productivity


• The world’s first and only fully automated front-end FISH processing system • Run up to 40 slides under multiple protocols• Reduce hands-on tech time from 7.5 hours to 30 minutes


• eFISHiency Workstation for manual FISH assay• Hybridizer with eXACTTM temperatures• 10 Independently programmable thermal cyclers• Built-in touch screen display• Manual coverslip application and removal

33 Steps Reduced to 3


• On-board dewaxing, oil seal and final coverslip after DAPI• Add micro-reagents manually to save cost• Run 10 different protocols at the same time• Intelligent SMS information for alerts


Accessories

Oil stamp Coverslip stand Suction pen

8

Automated Systems

www.biogenex.com

• Clean, crisp and intense stains

• High throughput – Up to 200 slides in eight-hour shift, 60 slides in 3 hours

• Over 300+ optimized protocols with ready-to-use reagents in barcoded vials

• Dispense reagents as low as 100µL/slide

• Multiple slide processing options - Random, Continuous and STAT

• Multi-format specimen processing - FFPE or frozen tissues, cell preparations, fine needle aspirates, smears and more…

• Color-coded GUI with real-time assay parameter display for all slides

• Customized or standard reports for inventory management and regulatory compliance and submission

Walkaway IHC Staining System

Multiplex IHC System

9

Automated Systems

[email protected]

• Fully open system to customize any manual protocol

• Simultaneous optimization of up to 60 assay parameters

• Disposable pipette tips – eliminates cross contamination

• Audio and visual alerts at every step for manual intervention

• Customized reporting system for detailed report generation

• Multiple slide processing options – Random, Continuous and STAT

Multi-functional System - Multiplex IHC, ISH, IF and Special Stains

Multifunctional Staining System for Research

10

Automated Systems

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LIS Enabled Barcode Label Printer

• For printing chemical resistant barcode labels

• Compatible with Xmatrx® and i6000TM

• User-friendly software

• Synchronization of protocol information

• Efficient system

• Eliminates human error

• Helps reduce operating cost

• Fast turn-around

Integrated Digitized Data Tracking System

11

Automated Systems

[email protected]

Primary AntibodiesProduct Name Cat. No. Clone/Source Pre-treatment

Aberrant Endothelial Cell AX382-YCD 4A11/Mouse HX031-YCDE

ACTH AX487-YCD AH26/Mouse HX031-YCDE

Actin Muscle-specific AX090-YCD HHF35/Mouse None

Actin Smooth muscle AX128-YCD 1A4/Mouse None

Adenovirus AX059-YCDE A62020069P/Mouse HX031-YCDE/HX032-YCDE

Alpha 1 Antitrypsin AW015-YCD Polyclonal/Rabbit HX031-YCDE/HX032-YCDE

Alpha Actinin AX097-YCD JLN20/Mouse HX031-YCDE/HX032-YCDE

Alpha-1-Antichymotrypsin AX388-YCD α1A88/Mouse HX031-YCDE

Alpha-Fetoprotein (AFP) AX008-YCD C3/Mouse None

Androgen Receptor AX256-YCDE F39.4.1/Mouse HX032-YCDE

Apolipoprotein E AX567-YCD EP1373Y/Rabbit HX031-YCDE/HX032-YCDE

B Cell AX158-YCD MB2/Mouse HX031-YCDE

B Lymphocyte antigen 36 (BLA-36) AX231-YCD A27-42/Mouse HX031-YCDE/HX032-YCDE

Bax Protein AW347-YCD Polyclonal/Rabbit HX031-YCDE/HX032-YCDE

Breast Cancer Antigen BCA-225 AX135-YCD CU18/Mouse HX031-YCDE

Bcl 2 AX541-YCD E17/Rabbit HX031-YCDE

Bcl 2 AY723-YCD EP36/Rabbit None

Bcl-2 Oncoprotein AX287-YCD Bcl-2/100/Mouse HX031-YCDE

Bcl-6 AY708-YCD LN22/Mouse None

Bcl-x AX462-YCD E18/Rabbit HX031-YCDE

Beta-Catenin AX510-YCD E247/Rabbit HX031-YCDE/HX032-YCDE

bFGF (Basic growth Factor) AX359-YCD bFGF88/Mouse HX031-YCDE

BOB-1 (New) AY731-YCDE SP92/Rabbit None

BRCA1 Protein AW345-YCD Polyclonal/Rabbit HX031-YCDE/HX032-YCDE

CA19-9 AX424-YCD C241:5:1:4/Mouse HX032-YCDE

CA 125 (Ovarian Tumor Marker) AX429-YCD Ov185:1/Mouse HX031-YCDE/HX032-YCDE

Caldesmon AX507-YCD E89/Rabbit HX031-YCDE

Caldesmon, High MW, Smooth muscle AX332-YCD h-CD/Mouse HX031-YCDE

Calponin AX333-YCD CALP/Mouse HX031-YCDE

Calponin-1 (C-term) AX502-YCD EP798Y/Rabbit HX031-YCDE

Calretinin AX583-YCD 2 E7/Mouse HX031-YCDE

Calretinin AW413-YCD Polyclonal/Rabbit HX031-YCDE

Carcinoembryonic Antigen (CEA) AX009-YCD B01-94-11M-P/Mouse None

Carcinoembryonic Antigen (CEA) AW009-YCD Polyclonal/Rabbit HX031-YCDE

Catenin (p120) AX523-YCD YE372/Rabbit HX031-YCDE/HX032-YCDE

Catenin Delta 1 (p120) AW706-YCD Polyclonal/Rabbit HX031-YCDE

Cathepsin D AX467-YCD C15/Mouse HX031-YCDE

Product Name Cat. No.

Xmatrx® ELITE AS4040B

Xmatrx® Infinity AS4000RX

Xmatrx® NANO AS1000

Xmatrx® MINI AS1010

Xmatrx® ULTRA AS4030B

Reagents and Consumables Immunohistochemistry – Reagents Primary antibodies in RFID tagged vials for 200 slides

Xmatrx® Automated Staining System

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Automated Systems

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Xmatrx® Reagents and Consumables Product Name Cat. No. Clone/Source Pre-treatment

CD1a AX490-YCD O10/Mouse HX031-YCDE/HX032-YCDE

CD2 AX438-YCD AB75/Mouse HX032-YCDE

CD3 Epsilon AX477-YCD EP449E/Rabbit HX032-YCDE

CD3 (T cell) AX322-YCD PS1/Mouse HX031-YCDE

CD3 (T Cell) AX258-YCD UCHT1/Mouse HX031-YCDE

CD3 + CD20 AC559-YCD EP449E/Rabbit/L-26/Mouse HX031-YCDE/HX032-YCDE

CD4 AX421-YCD 4B12/Mouse HX031E-YCDE

CD4 (New) AY722-YCD EP204/Rabbit HX032E-YCDE

CD4 and CD8 Cocktail AC595-YCD 4B12+EP1150Y/Mouse+Rabbit HX031-YCDE/HX032-YCDE

CD5 AX512-YCD EP2952/Rabbit HX031-YCDE/HX032-YCDE

CD5 Monoclonal AX430-YCD 4C7/Mouse HX032-YCDE

CD7 AY702-YCD LP15/Mouse HX031-YCDE

CD8 AX422-YCD 1A5/Mouse HX032-YCDE

CD8 AX513-YCD EP1150Y/Rabbit HX031-YCDE/HX032-YCDE

CD8 (New) AY740-YCD SP16/Rabbit HX032-YCDE

CD8 (T Cell, Suppressor/Cytotoxic) AX261-YCD T8/Mouse HX031-YCDE/HX032-YCDE

CD10 AX451-YCD 56C6/Mouse HX031-YCDE/HX032-YCDE

CD10 + Cyclin D1 AC561-YCD 56C6/Mouse/EPR2241/Rabbit HX031-YCDE/HX032-YCDE

CD11b (C3bi Receptor) AX270-YCD M01/Mouse HX031-YCDE/HX032-YCDE

CD11b/ITAM AX546-YCD EPR1344/Rabbit HX031-YCDE

CD11c AX555-YCD EP1347Y/Rabbit HX031-YCDE/HX032-YCDE

CD14 AX515-YCD EPR3653/Rabbit HX031-YCDE

CD15 (Blood group antigen Lewis X) AX302-YCD BRA4F1/Mouse None

CD16 AX437-YCD 2H7/Mouse HX032-YCDE

CD19 (New) AY729-YCD EP169/Rabbit HX031-YCDE

CD20 AX537-YCD CD20/C23/Mouse HX031-YCDE/HX032-YCDE

CD20 (B cell) AX238-YCD L-26/Mouse None

CD20 (B Cell) AX265-YCD B1/Mouse HX031-YCDE/HX032-YCDE

CD21 AX497-YCD EP3093/Rabbit HX031-YCDE/HX032-YCDE

CD21 (B Cell) AX266-YCD B2/Mouse HX031-YCDE/HX032-YCDE

CD22 AX439-YCD FPC1/Mouse HX031-YCDE

CD23 AW460-YCD Polyclonal/Rabbit HX031-YCDE

CD25 AX453-YCD 4C9/Mouse HX031-YCDE/HX032-YCDE

CD29 (Integrin beta-1 subunit) AX298-YCD JB1a/Mouse HX032-YCDE

CD30 (Ki-1 antigen) AX327-YCD Ber-H2/Mouse HX031-YCDE

CD30 (Ki-1 antigen) AX351-YCD HRS-4/Mouse HX031-YCDE

CD31 (Endothelial cell) AX232-YCD JC/70A/Mouse HX032-YCDE

CD31 (PECAM-1) AX241-YCD 9G11/Mouse HX031-YCDE/HX032-YCDE

CD34 (Endothelial cell) AX236-YCD QBend/10/Mouse None

CD35 AX431-YCD RLB25/Mouse HX031-YCDE/HX032-YCDE

CD35 (New) AY741-YCD SP191/Rabbit None

CD41/Integrin Alpha IIb (New) AY732-YCDE EP178/Rabbit None

CD43 & CD45RA AX159-YCD MT1 & MB1/Mouse HX031-YCDE/HX032-YCDE

CD43 (T cell Leukosialin) AX305-YCD DFT-1/Mouse HX031-YCDE

CD44 (Phagocytic glycoprotein-1 HCAM) AX310-YCD DF1485/Mouse None

CD45 AX111-YCD PD7/26/16&2B11/Mouse None

CD45 AX338-YCD LJ27.9/Mouse HX031-YCDE/HX032-YCDE

CD45 cocktail (Leukocyte common antigen LCA) AX371-YCD MEM55+LJ 24.9/Mouse None

CD45RA (B cell) AX157-YCD MB1/Mouse None

CD45RB AX320-YCD MEM55/Mouse HX031-YCDE/HX032-YCDE

13

Automated Systems

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Xmatrx® Reagents and Consumables Product Name Cat. No. Clone/Source Pre-treatment

CD45RC (T cell) AX156-YCD MT2/Mouse None

CD45RO (T cell) AX113-YCD UCHL-1/Mouse None

CD48 (New) AY721-YCDE EP148/Rabbit None

CD53 (New) AY734-YCD EP179/Rabbit None

CD56 (Natural Killer cells, NCAM) AX268-YCD NKH-1/Mouse HX031-YCDE/HX032-YCDE

CD57 (Natural killer cell) AX314-YCD NK-1/Mouse None

CD61 AX482-YCD EP2417Y/Rabbit HX031-YCDE

CD63 (New) AY720-YCDE EP211/Rabbit HK031-YCDE

CD66 AX325-YCD BY114/Mouse None

CD68 AX416-YCD KP1/Mouse HX031-YCDE

CD68 AX549-YCD CD68/G2/Mouse HX031-YCDE

CD71 (Transferrin Receptor) AX269-YCD T9/Mouse HX031-YCDE/HX032-YCDE

CD71 (Transferrin Receptor) AX354-YCD H68.4/Mouse HX031-YCDE/HX032-YCDE

CD74 (B Cell) AX153-YCD LN2/Mouse HX031-YCDE/HX032-YCDE

CD79a AX414-YCD 11E3/Mouse HX032-YCDE

CD79a (New) AY719-YCD EP82/Rabbit HK031-YCDE



CD99 AX518-YCD EPR3097Y/Rabbit HX031-YCDE/HX032-YCDE

CD99 (MIC2 antigen) AX355-YCD HO36.1.1/Mouse HX032-YCDE

CD103 (New) AY739-YCDE EP206/Rabbit None

CD105 AX441-YCD 4G11/Mouse HX031-YCDE/HX032-YCDE

CD117 AX423-YCD T595/Mouse HX031-YCDE

CD117 AX465-YCD YR145/Rabbit HX031-YCDE/HX032-YCDE

CD146 (New) AY716-YCD EP54/Rabbit HX032-YCDE

CD205 (New) AY737-YCDE EP176/Rabbit HX031-YCDE

CD227 (Mucin 1) AX534-YCD YU-4H5/MOUSE HX031-YCDE

CDw75 (B Cell) AX152-YCD LN1/Mouse HX031-YCDE/HX032-YCDE

CDX-2 AX392-YCD CDX2-88/Mouse HX032-YCDE

CDX-2, CK20 and CK7 Cocktail AC597-YCD CDX2-88+EPR1622Y+EPR1619Y/Mouse+Rabbit HX031-YCDE/HX032-YCDE

CDX-2 and CK7 Cocktail AC596-YCD CDX2-88+EPR1619Y/Mouse+Rabbit HX031-YCDE/HX032-YCDE

CEA AX365-YCD CEA88/Mouse None

c-erbB-2 (HER-2/neu) AX471-YCDE EP1045Y HX031-YCDE

c-erbB-2 (Her-2/neu) AX134-YCDE CB11/Mouse None

c-erbB-2 (HER-2/neu) (New) AY726-YCDE EP3/Rabbit None

c-erbB-3 (HER-3) AX319-YCD RTJ1/A2/Mouse HX031-YCDE/HX032-YCDE

Chromogranin A AX126-YCD LK2H10/Mouse None

Chromogranin A AX356-YCD PHE-5/Mouse HX031-YCDE

Claudin 1 AW704-YCD Polyclonal/Rabbit HX031-YCDE

Claudin-5 (New) AY718-YCD EP224/Rabbit HX032-YCDE

c-myc Protein AX318-YCD 9E10/Mouse HX032-YCDE

Collagen III AX167-YCD HWD1.1/Mouse None

Collagen IV AX379-YCD COL-94/Mouse HX031-YCDE/HX032-YCDE

Cyclin D1 AW447-YCD Polyclonal/Rabbit HX031-YCDE

Cyclin D1 AX474-YCD EPR2241(IHC)-32/Rabbit HX032-YCDE

Cyclin E2 AX519-YCD EP454Y/Rabbit HX032-YCDE

Cytokeratin 4 AY705-YCD 6B10/Mouse None

Cytokeratin 4 (New) AY717-YCD EP4/Rabbit HX031-YCDE

Cytokeratin 5 AX484-YCD EPR1600Y/Rabbit HX031-YCDE

Cytokeratin 5 & 6 AX588-YCD EPR1600Y & EPR1602Y/Rabbit HX031-YCDE

Cytokeratin 5 & Cytokeratin 14 (New) AY730-YCDE EP24/EP61/Rabbit HX032-YCDE


14

Automated Systems

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Product Name Cat. No. Clone/Source Pre-treatment

Cytokeratin 7 AX255-YCD OV-TL12/30/Mouse HX032-YCDE


Cytokeratin 7 & 8 AX587-YCD OV-TL12/30 & C51/Mouse HX031-YCDE

Cytokeratin 8 AX142-YCD C51/Mouse HX031-YCDE

Cytokeratin 8 & 18 AX131-YCD 5D3/Mouse HX031-YCDE

Cytokeratin 10 AX201-YCD DEK-10/Mouse HX031-YCDE

Cytokeratin 13 AX132-YCD AE8/Mouse HX031-YCDE

Cytokeratin 14 AX146-YCD LL002/Mouse HX031-YCDE


Cytokeratin 16 AX486-YCD EP1615Y/Rabbit HX031-YCDE

Cytokeratin 17 AX572-YCD E27/Mouse HX031-YCDE

Cytokeratin 18 AX143-YCD DC-10/Mouse HX031-YCDE

Cytokeratin 19 AX246-YCD RCK108/Mouse HX032-YCDE

Cytokeratin 20 AX315-YCD IT-Ks20.8/Mouse HX031-YCDE


Cytokeratin cocktail AX071-YCD AE1 and AE3/Mouse HX031-YCDE

Cytokeratin cocktail, Broad spectrum AX273-YCD 34ßE12/C51/AE1/Mouse HX032-YCDE

Cytokeratin cocktail, Broad spectrum AX372-YCD LL002+DEK-10+RCK108+OVTL12/30+C11/Mouse HX032-YCDE

Cytokeratin High MW AX291-YCD 34ßE12/Mouse HX031-YCDE

Cytokeratin High MW (Basic) AX133-YCD AE3/Mouse HX031-YCDE

Cytokeratin Low MW AX075-YCD AE1/Mouse HX032-YCDE

Cytokeratin Pan AX181-YCD Lu-5/Mouse HX031-YCDE

Cytokeratin Pan AX357-YCD C11/Mouse HX031-YCDE

Desmin AX072-YCD D33/Mouse None

DOG 1 AX570-YCD 1.1/Mouse HX031-YCDE

Dystrophin AX243-YCD Dy4/6D3/Mouse HX031-YCDE

Dystrophin AX244-YCD Dy8/6C5/Mouse HX031-YCDE

E-Cadherin AX390-YCD 36/Mouse HX031-YCDE

E-Cadherin (New) AY725-YCD EP6/Rabbit HX032-YCDE

EGFR AW335-YCDE Polyclonal/Rabbit HX031-YCDE/HX032-YCDE

Ep-CAM AX489-YCD E144/Rabbit HX031-YCDE/HX032-YCDE

Epithelial Membrane Antigen (EMA) AX057-YCD E29/Mouse HX031-YCDE/HX032-YCDE

Epithelial Membrane Antigen (EMA) AX182-YCD Mc5/Mouse HX031-YCDE

Epithelial-Specific Antigen AX316-YCD MOC-31/Mouse EK000-5K

Estradiol AW038-YCD Polyclonal/Rabbit HX031-YCDE

Estrogen Receptor AY710-YCDE EP-1/Rabbit None

Estrogen Receptor AX368-YCDE ER88/Mouse HX031-YCDE

Estrogen Receptor AX509-YCDE EPR703(2)/Rabbit HX032-YCDE

Estrogen Receptor AX272-YCDE 1D5/Mouse HX032-YCDE

Estrogen Receptor-beta AW385-YCD Polyclonal/Rabbit HX031-YCDE/HX032-YCDE

Factor VIII-related antigen AX016-YCD F8 2.2.9/Mouse HX031-YCDE

Factor XIII Subunit A AX516-YCD EP3372/Rabbit HX031-YCDE

Factor XIII A AX337-YCD E980.1/Mouse HX031-YCDE

Fascin AX488-YCD FCN01/Mouse HX031-YCDE

Fibronectin AX569-YCD F14/Rabbit HX031-YCDE/HX032-YCDE

Follicle Stimulating Hormone (FSH) AW026-YCD Polyclonal/Rabbit HX031-YCDE

Gastrin AW019-YCD Polyclonal HX031-YCDE

GCDFP 15 AX481-YCD EP1582Y/Rabbit HX031-YCDE

Glial fibrillary acidic protein (GFAP) AX020-YCD GA-5/Mouse None

Glial Fibrillary Acidic Protein (GFAP) AW020-YCD Polyclonal/Rabbit HX031-YCDE

Glomerular epithelial Protein 1 (GLEPP-1) AX336-YCD 5C11/Mouse HX031-YCDE/HX032-YCDE

Glucagon AW039-YCD Polyclonal/Rabbit HX031-YCDE

Xmatrx® Reagents and Consumables

15

Automated Systems

[email protected]


Glut-1 AX505-YCD SPM498/Mouse HX031-YCDE/HX032-YCDE

Glutathione S-transferase Pi (GST Pi) AW249-YCD Polyclonal/Rabbit HX031-YCDE/HX032-YCDE

HSA AX550-YCD HSA/E8/Mouse HX031-YCDE

Heat Shock Protein (HSP-70) AX289-YCD BRM-22/Mouse HX031-YCDE/HX032-YCDE

Heat shock protein 27 (HSP 27) AX171-YCD G3.1/Mouse HX031-YCDE

Hemoglobin A AW021-YCD Polyclonal/Rabbit HX031-YCDE

HGH AW707-YCD Polyclonal/Rabbit HX031-YCDE

HLA-DR AX154-YCDE LN3/Mouse HX031-YCDE/HX032-YCDE

Human Chorionic Gonadotropin (HCG) Beta AX395-YCD M94138/Mouse HX031-YCDE

IgA AW045-YCD Polyclonal/Rabbit HX031-YCDE/HX032-YCDE

IgD AW440-YCD Polyclonal/Rabbit HX031-YCDE/HX032-YCDE

IgG AW050-YCD Polyclonal/Rabbit HX031-YCDE

IgG AX367-YCD IgG88/Mouse HX031-YCDE/HX032-YCDE

IgM AW427-YCD Polyclonal/Rabbit HX031-YCDE/HX032-YCDE

IgM AX366-YCD IgM88/Mouse HX031-YCDE/HX032-YCDE

Inhibin Alpha AX446-YCD R1/Mouse HX031-YCDE

Insulin AX029-YCD HB125/Mouse HX031-YCDE

Insulin (New) AY735-YCD EP125/Rabbit None

J chain AX374-YCD JC88/Mouse HX031-YCDE

Kappa Light Chain AX369-YCD K88/Mouse HX031-YCDE

Kappa light chain AX048-YCD L1C1/Mouse HX031-YCDE

Ki-67 AX527-YCD EPR3611/Rabbit HX032-YCDE

Ki-67 AX410-YCD K2/Mouse HX032-YCDE

Ki-67 (New) AY727-YCD EP5/Rabbit None

Ki-67 antigen AX370-YCD Ki88/Mouse HX032-YCDE

Ki-67 antigen Proliferating cell AX297-YCD MIB-1/Mouse HX032-YCDE

Ki-67 + Lambda Light Chain AC562-YCD K-2/Mouse+Polyclonal/Rabbit HX031-YCDE/HX032-YCDE

Lambda Light chain AW049-YCD Polyclonal/Rabbit HX031-YCDE

Lambda Light Chain (New) AY715-YCD EP172/Rabbit HX032-YCDE

Laminin AW078-YCD Polyclonal/Rabbit HX031-YCDE/HX032-YCDE

Lysozyme AW024-YCD Polyclonal/Rabbit HX031-YCDE

Macrophage AX165-YCD LN5/Mouse HX031-YCDE

Mast Cell Tryptase AX419-YCD AA1/Mouse HX031-YCDE

Melan-A (MART-1) AX361-YCD A103/Mouse HX031-YCDE

Melanoma AX001-YCD HMB45/Mouse HX031-YCDE

Melanoma-Associated Antigen AX077-YCD NKI/C3 HX031-YCDE/HX032-YCDE

Melanoma gp100 AX536-YCD gp100/D5/Mouse HX031-YCDE

Mesothelin AX433-YCD 5B2/Mouse HX031-YCDE

MiTF AX554-YCD MiTF/A13/Mouse HX032-YCDE

MLH1 AY703-YCD ES05/Mouse HX031-YCDE

MMP9 AX504-YCD EP1255Y/Rabbit HX031-YCDE

MSH2 (New) AY743-YCD SP46/Rabbit None

MSH6 AX454-YCD 2D4B5/Mouse HX032-YCDE

MUC4 AX455-YCD 1G8/Mouse HX031-YCDE/HX032-YCDE

MUC5AC AX456-YCD 45M1/Mouse HX031-YCDE

Mucin 1 AX564-YCD EPR1023/Rabbit HX031-YCDE

Mucin-2 (MUC-2) AX358-YCD CCP58/Mouse HX031-YCDE

Multi-Drug Resistance Marker (P-Glycoprotein) AX391-YCD MDR88/Mouse HX031-YCDE/HX032-YCDE

Muscle actins AX381-YCD Actin 88 Cocktail/Mouse HX031-YCDE


16

Automated Systems

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Myelin Basic Protein AX380-YCD MBP88/Mouse None

Myeloid Specific Antigen AX216-YCD BM-3/Mouse HX031-YCDE

Myeloid Specific antigen (BM-1) AX164-YCD BM-1/Mouse HX032-YCDE

Myeloperoxidase (MPO) AW496-YCD Polyclonal/Rabbit HX031-YCDE

Myf4 AX432-YCD LO26/Mouse HX032-YCDE

Myoglobin AX012-YCD MG-1 HX031-YCDE

Myoglobin AW012-YCD Polyclonal/Rabbit HX031-YCDE

Myosin, Skeletal Muscle AX109-YCD MY-32/Mouse HX031-YCDE/HX032-YCDE

Myosin Heavy Chains, Smooth Muscle AX331-YCD SMMS.1/Mouse HX032-YCDE

N-Cadherin AX565-YCD EPR1792Y/Rabbit HX031-YCDE/HX032-YCDE

Napsin A AY701-YCD IP64/Mouse HX031-YCDE

Neurofilament AX073-YCD NE-14/Mouse None

Neuron specific enolase (NSE) AX055-YCD MIG-N3/Mouse None

NGFR AX522-YCD EP1039Y/Rabbit HX031-YCDE

NGFR (New) AY738-YCD EP31/Rabbit HK032-YCDE

Oct 2 AX558-YCD EPR542/Rabbit HX032-YCDE

Oct 4 (New) AY724-YCD EP143/Rabbit HK032-YCDE

Osteonectin AX387-YCD OST1/Mouse HX031-YCDE

p105 Proliferation-Associated Nuclear Antigen AX317-YCD 2B3/Mouse HX032-YCDE

p21/WAF1 AX434-YCD 4D10/Mouse HX032-YCDE

p27 (Kip1) AX492-YCD Y236/Rabbit HX032-YCDE

p27 (Kip1) AX396-YCD DCS72/Mouse HX032-YCDE

p34cdc2 (Cyclin Dependent Kinase) AX301-YCD POH-1/Mouse HX031-YCDE/HX032-YCDE

P504S AX449-YCDE 13H4 HX031-YCDE

P504S AX538-YCD RBT-AMACR/Rabbit HX031-YCDE

p53 (New) AY728-YCD EP9/Rabbit None

p53 Protein AX195-YCD BP53-12-1/Mouse HX032-YCDE

p53 Protein AX239-YCD DO7/Mouse HX032-YCDE

p53 Protein AX240-YCD 1801/Mouse HX032-YCDE

p63 Protein AX418-YCD 4A4/Mouse HX032-YCDE

p63+CK5 AC602-YCD 4A4/Mouse + EPR1600Y/Rabbit HX031-YCDE/HX032-YCDE

PAP AX532-YCD A40010/Mouse HX031-YCDE

Papillomavirus type 16 (HPV-16) AX362-YCDE Cam Vir-1/Mouse HX032-YCDE

PAX5 AX457-YCD ZP007/Mouse HX032-YCDE

Paxillin AX521-YCD Y113/Rabbit HX031-YCDE/HX032-YCDE

PDCD4 AX524-YCD EPR3432/Rabbit HX031-YCDE/HX032-YCDE

PDGF AW376-YCD PDGF88 None

PDGFR Beta AX463-YCD Y92/Rabbit HX031-YCDE

PGP9.5 (New) AY736-YCDE 3D9/Mouse HK031-YCDE

PIN4 (p63 + CK HMW + P504S) AX448-YCDE Mouse4A4+Mouse34βE12+Rabbit13H4 HX032-YCDE

Placental alkaline phosphatase (PLAP) AX228-YCD PL8-F6/Mouse None

Placental Lactogen (hPL) AW040-YCD Polyclonal/Rabbit HX031-YCDE

Platelet derived growth factor (PDGF) AX376-YCD PDGF88/Mouse HX031-YCDE

PMS2 AX577-YCD EPR3947/Rabbit HX032-YCDE

Prealbumin/Transthyretin AX501-YCD EP2929Y/Rabbit HX031-YCDE

Progesterone receptor AX172-YCDE 1Ab/Mouse HX032-YCDE

Progesterone receptor AX328-YCD PR88/Mouse HX032-YCDE

Progesterone receptor AY711-YCDE EP2/Rabbit HX031-YCDE

Prolactin AX031-YCD ME.121/Mouse None

Proliferating cell nuclear antigen (PCNA) AX252-YCD PC10/Mouse HX032-YCDE

Prostate specific acid phosphatase (PSAP) AX013-YCDE B01-94-21M-NA/Mouse None

Prostate specific antigen (PSA) AX014-YCDE ErPr-8/Mouse None


17

Automated Systems

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pS2 Estrogen inducible protein AX190-YCD PS2.1/Mouse HX031-YCDE

PSMA (New) AY714-YCD EP192/Rabbit HK032-YCDE

RCC AX543-YCD RCC-26/Mouse None

S100 beta (New) AY713-YCD EP32/Rabbit HK032-YCDE

S-100 Protein AX058-YCD 15E2E2/Mouse None

S-100 Protein AW058-YCD Polyclonal/Rabbit HX031-YCDE/HX032-YCDE

S100P (New) AY712-YCD EP186/Rabbit None

Sarcomeric Actin AX511-YCD ZMSA-5/Mouse HX031-YCDE

Secretin AW067-YCD Polyclonal/Rabbit HX031-YCDE

Stat 5 Alpha AX480-YCD E289/Rabbit HX031-YCDE/HX032-YCDE

Substance P AW069-YCD Polyclonal/Rabbit HX031-YCDE/HX032-YCDE

Survivin AX503-YCD EP2880Y/Rabbit HX032-YCDE

Synaptophysin AX363-YCD Snp88/Mouse HX031-YCDE

TAU AX412-YCD Tau-2/Mouse HX031-YCDE

Tau AX459-YCD TAU-5/Mouse HX031-YCDE

Terminal deoxynucleotidyl transferase (TdT) AX373-YCD TdT88/Mouse HX031-YCDE

Terminal deoxynucleotidyl Transferase (TdT) AW373-YCD Polyclonal/Rabbit HX032-YCDE

TFPU.1 AX520-YCD EPR3159Y/Rabbit HX032-YCDE

Thyroglobulin AX032-YCD 2H11/Mouse None

Thyroid stimulating hormone (TSH) AX033-YCD 5404/Mouse None

Thyroid stimulating hormone (TSH) AW033-YCD Polyclonal/Rabbit HX031-YCDE

Thyroxine AX034-YCD D5/Mouse HX031-YCDE/HX032-YCDE

TIA-1 AX529-YCD 2G9A10F5/Mouse HX031-YCDE

Topo 2 Alpha AX576-YCD EP1102Y/Rabbit HX032-YCDE

Transferrin AX025-YCD HT1/13.6.3/Mouse HX031-YCDE

Transforming growth factor (TGF), alpha AX377-YCD TGF-α88/Mouse HX031-YCDE

TTF-1 AX397-YCD 8G7G3/1/Mouse HX031-YCDE

TTF-1 + CK20 + CK7 AC605-YCD 8G7G3/1+EPR1622 Y+EPR1619Y/Mouse+Rabbit HX031-YCDE/HX032-YCDE

TTF-1 + GCDFP-15 AC604-YCD 8G7G3/1+EP1582Y/Mouse+Rabbit HX031-YCDE/HX032-YCDE

TTF1+CK5/6 AC563-YCD 8G7G3/1/Mouse+EPR1600Y&EPR1602Y/Rabbit HX031-YCDE/HX032-YCDE

Tumor-associated glycoprotein (TAG-72) AX054-YCD B72.3/Mouse None

Tumor-Associated glycoprotein (TAG-90, BCA) Dx AX005-YCD B6.2/Mouse HX031-YCDE

Tyrosinase AX535-YCD Ty/G5/Mouse HX031-YCDE

VEGF AW483-YCD Polyclonal/Rabbit HX031-YCDE

Villin AX525-YCD ID2C3/Mouse HX031-YCDE

Vimentin AX074-YCD V9/Mouse None

Vimentin (Non-hematopoietic) AX163-YCD LN6/Mouse None

VIP AW530-YCD Polyclonal/Rabbit HX031-YCDE

Wilm’s Tumor AX584-YCD CAN-R9(IHC)-56-2/Rabbit HX032-YCDE

ZAP-70 AX544-YCD ZAP70-C3/Mouse HX031-YCDE

ZAP-70 AX466-YCD YE291/Rabbit HX031-YCDE/HX032-YCDE

For pretreatment information, please contact us.


18

Automated Systems

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Product Name Pack size Cat. No.

XVizTM Detection Kit for Xmatrx® Diagnostics

EZ-ARTM Elegance solutions (1 X 16 ml each of solutions 1, 2, and 3) 3 X 16 ml Peroxide Block, 3 X 16 ml Power BlockTM, 1 X 16 ml Super Enhancer, 1 X 16 ml Polymer HRP, 4 X 11 ml DAB Buffer, 1 X 4 ml DAB chromogen, 3 X 16 ml Hematoxylin

200 slides QD550-YCDE

XVizTM Detection Kit for Xmatrx® Infinity

EZ-ARTM Elegance Solution (1x16 ml solution), 4x16 ml Peroxide Block, 1x15 ml Super enhancer, 5x11 ml DAB buffer, 1x4 ml DAB Chromogen, 1x15 ml Polymer HRP, 4x16 ml Hematoxylin, 1x21 ml Power Block.

200 slides QD550-YCX

All reagents are packed in RFID tagged vials especially designed for use on Xmatrx® Automated Staining Systems to ensure accurate identification, proper reagent inventory management and staining up to 200 slides.

The XVizTM Detection System

Super SensitiveTM One-step Polymer-HRP Detection Kit

Product Name Pack size Contents Cat. No.

Super SensitiveTM One-step Polymer-HRP Detection Kit/DAB

200 Slides EZ-ARTM Elegance solutions (1 x 16ml each of solutions 1, 2, and 3), 3 x 16ml Peroxide Block, 3 x 16ml Power BlockTM, 1 x 16ml Polymer HRP, 4 x 11ml DAB Buffer, 1 x 4ml DAB chromogen, 3 x 16ml Hematoxylin

QD610-YADE

This kit is designed with the proprietary technology which provides superior sensitivity, specificity and very short protocol. The innovative secondary antibody-polymer conjugate consists of multiple small HRP active sites, which enables clean and intense, nuclear, cytoplasmic, and membrane stains.

Product Name Pack Size Contents Cat. No.

XViz™ Double Staining Polymer Detection Kit I/DAB&Fast Red

100 Slides

2 X 10 ml Power Block, 2 X 10 ml Peroxide Block, 4 X 5 ml DAB Buffer 1 X 3 ml Liquid DAB Chromogen, 1 X 7 ml EZ-AR™ Elegance Solutions (1, 2 And 3), 1 X 7 ml Mouse Negative Control, 1 X 7 ml Rabbit Negative Control, 1 X 7 ml Anti Rabbit Poly-Hrp + Anti Mouse Poly-Hrp, 2 X 10 ml Hematoxylin, 2 X 14 ml Permanent Fast Red A, 2 X 14 ml Permanent Fast Red B

QS200-YADE

XViz™ Double Staining Polymer Detection Kit II/DAB&Fast Red

100 Slides

2 X 10 ml Power Block, 2 X 10 ml Peroxide Block, 4 X 5 ml DAB Buffer 1 X 3 ml Liquid DAB Chromogen, 1 X 7 ml EZ-AR™ Elegance Solutions (1, 2 And 3), 1 X 7 ml Mouse Negative Control, 1 X 7 ml Rabbit Negative Control, 1 X 7 ml Anti Mouse Poly-Hrp + Anti Rabbit Poly-Hrp, 2 X 10 ml Hematoxylin, 2 X 14 ml Permanent Fast Red A, 2 X 14 ml Permanent Fast Red B

QS400-YADE

XVizTM Double Staining Polymer Detection Kits

Immunohistochemistry - Detection Kits


Auto DAB kit for Xmatrx EliteProduct Name Pack size Contents Cat. No.

Auto DAB kit for Xmatrx Elite 200 slides 1 vial of DAB chromogen

5 empty vials for DAB mixing

XT150-YCDE

19

Automated Systems

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Antigen Retrieval SolutionsThe EZ-ARTM Elegance Solutions possess unique properties that enable optimal dewaxing, rehydration, and antigen retrieval in formalin-fixed, paraffin-embedded tissue sections. These solutions facilitate the production of highly reproducible and superior quality stains in a considerably short period of time without compromising the morphology and antigenicity of the tissue.

Product Name Pack size Product Description Cat. No.

EZ-AR™ 1 Elegance 200 slides EZ-AR™ 1 Elegance is a Citra based solution. Works at 100°C HX031-YCDE

EZ-AR™ 2 Elegance 200 slides EZ-AR™ 2 Elegance is an EDTA based solution. Works at 100°C HX032-YCDE

EZ-AR™ 3 Elegance 200 slides EZ-AR™ 3 Elegance is a Citra based solution. Works at 100°C HX033-YCDE

EZ-AR™ 4 Elegance 200 slides EZ-AR™ 4 Elegance is an Tris based solution. Works at 100°C HX034-YCDE



EZ-AR™ 1 Elegance 200 slides EZ-AR™ 1 Elegance is a Citra based solution. Works at 100°C HX031-YCX

EZ-AR™ 2 Elegance 200 slides EZ-AR™ 2 Elegance is an EDTA based solution. Works at 100°C HX032-YCX

EZ-AR™ 3 Elegance 200 slides EZ-AR™ 3 Elegance is a Citra based solution. Works at 100°C HX033-YCX

EZ-AR™ 4 Elegance 200 slides EZ-AR™ 4 Elegance is an Tris based solution. Works at 100°C HX034-YCX

Enzymatic Pre-treatment SolutionsProduct Name Pack size Cat. No.

Pepsin 4-Pack: 4 vials of Lyophilized Enzyme Powder, 4 x 6 mL Reconstitution Buffer 200 slides EK000-10X

Trypsin 4-Pack: 4 vials of Lyophilized Enzyme Powder, 4 x 6 mL Reconstitution Buffer 200 slides EK001-10X

Protease XXIV 4-Pack: 4 vials of Lyophilized Enzyme Powder, 4 x 6 mL Reconstitution Buffer 200 slides EK002-10X

in situ Hybridization Kits and ProbesThe XISH Detection Kit is designed for using with fluorescein labeled probes. It enables accurate detection of specific DNA and mRNA sequences in routine paraffin sections/cell smears.

Product Name Pack size Probe Type Cat. No.

XISHTM One Step Polymer-HRP ISH Detection System

1 x10ml Power Block, 1 x 10 ml Peroxide Block, 4 x 5 ml DAB Buffer, 1 x 3ml Liquid DAB Chromogen, 1 x 3ml One step Poly-HRP Reagent; 1 x 10ml Hematoxylin; 1 x 3ml Proteinase K; 1 x 3ml Nucliec Acid Retrieval Solution; 2 x 10ml Wash Solution A; 2 x 10ml Wash Solution B; 2 x 10ml Wash Soution E; 2 x 10ml Wash Solution F; 1 x 3ml Anti-Flourescein Antibody

50 slides Fluorescein Labeled DF400-YADE

One Step ISH Detection Kit

ISH ProbesProbes are packaged with RFID vials for staining up to 25 slides.

Product Name Pack size Intended Use Cat. No.

Alu II DNA 25 slides Positive control probe for detection of primate DNA sequence repeat PR026-YADE

CerviPro HPV 14 25 slides Detection of high risk genotypes of human papillomavirus PR251-YADE

CerviPro HPV Type 16/18 25 slides Detection of HPV types 16 and 18 PR250-YADE

Cyclin D1 25 slides Detection of CyclinD1 mRNA PR216-YADE

Epstein Barr Virus Early RNA (EBER) 25 slides Detection of latent EBV infection PR205-YADE

Kappa 25 slides Detection of Kappa light chain mRNA PR214-YADE

Lambda 25 slides Detection of Lambda light chain mRNA PR215-YADE

Oligo dT 25 slides Assessment of mRNA preservation PR217-YADE

Retinoblastoma 25 slides Detection of Retinoblastoma mRNA PR225-YADE

Beta-Actin 25 slides Internal standard for ISH and Northern blot PR1055-YADE

Note: See Page No. 151 for New ISH Probes (To be launched)

20

Automated Systems

www.biogenex.com

Product Name Pack Size Cat. No.

Barrier Slides, 18x18 mm, 2-zone, Xmatrx® ELITE & Infinity 1440 Slides/Case XT114-CL

Barrier Slides, 18x18 mm, 2-zone, Xmatrx® ELITE & Infinity 72 Slides/Box XT114-SL

Barrier Slides, 18x18 mm, Xmatrx® ELITE & Infinity 1440 Slides/Case XT128-CL

Barrier Slides, 18x18 mm, Xmatrx® ELITE & Infinity 72 Slides/Box XT128-SL

Barrier Slides, 25X25 mm, Xmatrx® ELITE & Infinity 1440 Slides/Case XT108-CL

Barrier Slides, 25X25 mm, Xmatrx® ELITE & Infinity 72 Slides/Box XT108-SL

Barrier Slides, 25X40 mm, Xmatrx® ELITE ISH & Infinity 1440 Slides/Case XT134-CL

Barrier Slides, 25X40 mm, Xmatrx® ELITE ISH & Infinity 72 Slides/Box XT134-SL

Coverslips, 18x18 mm, Xmatrx® ELITE & Infinity 1750 Coverslips/Case XT121-XBK

Coverslips, 18x18 mm, Xmatrx® ELITE & Infinity 175 Coverslips/Box XT121-YBX

Coverslips, 25x25 mm, Xmatrx® Infinity & ELITE ISH 90 Coverslips/Box XT122-90X

Coverslips, 25x25 mm, Xmatrx® Infinity & ELITE ISH 900 Coverslips/Case XT122-YQK

Coverslips, 25x40 mm, Xmatrx® ELITE & Infinity 50 Coverslips/Box XT118-50X

Coverslips, 25x40 mm, Xmatrx® ELITE & Infinity 500 Coverslips/Box XT118-YRK

Reagent Vials, Brown, 20ml, Xmatrx® 24/Pack XT126-24V

Reagent Vials, Translucent, 20ml 24/Pack XT026-V24

Pipette Tips, 1 ml, Xmatrx® ELITE & Infinity 960 Tips/Case XT104-05X

Pipette Tips, 1 ml, Xmatrx® ELITE & Infinity 192 Tips/Box XT105-01X

Xmatrx® ELITE and Infinity Consumables


User defined Empty RFID tagged vials- Two step IHC Each XT077-AX0601 to XT077-AX0800

User defined Empty RFID tagged vials- One step IHC Each XT077-AX0801 to XT077-AX0999

User defined Empty RFID tagged vials- ISH Probes Each XT079-PR0050 to XT079-PR0099

Empty Reagent Vials


Xflex Consumable Kit 3 x 72 nos 25 X 40 mm Double Barrier Slides, 2 x 500 nos 25 x 40 mm Coverslips, 9 x 96 nos Small Pipette tips, 2 x 192 Large Pipette tips (1 ml)

200 slides XT132-YCD

ISH Consumable Kit-Xmatrx® 2 x 72 nos 25 x 25 mm Double Barrier Slides, 1 x 900 Nos of 25 x 25 mm Coverslips, 2 x 192 Large Pipette Tips (1 ml), 1 x 960 Nos of Pipette Tips (200µL)

100 slides XT144-YAD

Xflex Ultra Consumable Kit 3 x 72 Nos of 25 X 40 mm Double Barrier Slides, 2 x 500 Nos of 25 X 40 CoverSlips, 1 X 960 Nos of Pipette Tips (200µL), 2 x 192 Large Pipette Tips (1 ml)

200 slides XT148-YCD

FISH Consumable Kit

2 x 72 nos 18 x 18 mm Barrier slides, 2 x 175 nos of 18 x 18 mm coverslips Xmatrx®, 1 x 960 pipette tips (200µL), 2 x 192 Large pipette tips (1ml)

100 slides XT147-YAD

Consumable Kit


21

Automated Systems

[email protected]

Wash BuffersXWashTM Buffer provides optimal staining with minimal background.


XWashTM, IHC Wash Buffer, 20X concentrated 500 ml HX020-YIK

XWashTM, ISH Wash Buffer, 20X concentrated 500 ml HX017-YIK

Super Sensitive™ SS Wash Buffer, 20X concentrated 500 ml HK583-5KE


Xmatrx® FISH Software 4812-00089

Xmatrx® FISH Application

DeWax Solutions†

BioGenex X-DeWaxTM Solution is a “one-step” product that simultaneously enables the removal of paraffin and allows rehydration of the tissue with a single reagent. In the past, formalin-fixed, paraffin-embedded tissue sections were traditionally deparaffinized with highly toxic, noxious chemicals (i.e. xylene, equivalents). BioGenex, a pioneer in the Immunohistochemistry technology, offers a xylene-free product that removes the paraffin from mounted tissue slides easily and rapidly.

Ancillary Reagents


X-DeWax™ Solution (Concentrated) 1000 ml HX016-XAK†

X-DeWax™ Solution (Concentrated) 1 Gallon HX016-XEK†


Pipette Tips, 200 ul, Xmatrx® Infinity & ELITE 960 Tips/Box XT146-01X

Pipette Tips, 200ul, Xmatrx® Infinity & ELITE 4800 Tips/Case XT145-05X

Reagent Vial Insert, 2 ml 24/Pack XT149-V24

Empty Reagent Vials for Xmatrx - White 24/Pack XT077-V24

Empty Reagent Vials for Xmatrx - Brown 24/Pack XT078-V24


XMount™ for Xmatrx® 40 slides HX035-04X

XMount™ for Xmatrx® 100 slides HX035-40D

XMount™ for Xmatrx® 100 slides HX035-10X

XMount™


† U.S. Patent No. 6,632,598; U.S. Patent No. 7, 070, 951; Japanese Patent No. 3532571; European Patent No. 0698118B1.

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Automated Systems

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Barrier Slides, 18x18 mm, 2-zone, Xmatrx® 1440 Slides/Case XT114-CL

Barrier Slides, 18x18 mm, 2-zone, Xmatrx® 72 Slides/Box XT114-SL

Barrier Slides, 18x18 mm, Xmatrx® 1440 Slides/Case XT128-CL

Barrier Slides, 18x18 mm, Xmatrx® 72 Slides/Box XT128-SL





Coverslips, 18x18 mm, Xmatrx® 1750 Coverslips/Case XT121-XBK

Coverslips, 18x18 mm, Xmatrx® 175 Coverslips/Box XT121-YBX

Coverslips, 25x25 mm, Xmatrx® 900 Coverslips/Case XT122-YQK

Coverslips, 25x25 mm, Xmatrx® 90 Coverslips/Box XT122-90X

Coverslips, 25x40 mm, Xmatrx® 500 Coverslips/Box XT118-YRK

Coverslips, 25x40 mm, Xmatrx® 50 Coverslips/Box XT118-50X

Reagent Vials, Translucent, 20 ml 24/Pack XT026-V24

Pipette Tips, 1 ml 960 Tips/Case XT104-05X

Pipette Tips, 1 ml 192 Tips/Box XT105-01X

Pipette Tips, 200 ul 960Tips/Box XT146-01X

Pipette Tips, 200 ul 4800 Tips/Box XT145-05X

Reagent Vial Insert, 2 ml 24/Pack XT149-V24

Reagent Vial, Brown, 20ml 24/Pack XT126-24V

FISH Consumable Kit-Xmatrx® 100 slides XT147-YAD

Note: Unless specified otherwise, all products listed in this section are for Laboratory Use Only.† U.S. Patent No. 6,632,598; U.S. Patent No. 7, 070, 951; Japanese Patent No. 3532571; European Patent No. 0698118B1.

Consumables for FISH


23

Automated Systems

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Reagents and ConsumablesImmunohistochemistry - Reagents Primary antibodies in 10 ml Barcoded vials

Primary AntibodiesProduct Name Cat. No. Clone/Source Pre-treatment

ACTH AM487-10M AH26/Mouse HK522-XAK

Actin, Muscle-Specific AM090-10M HHF35/Mouse None

Actin, Smooth Muscle AM128-10M 1A4/Mouse None

Alpha-Fetoprotein (AFP) AM008-10M C3/Mouse None

Androgen receptor AM256-10ME F39.4.1/Mouse HK087-5KE

Apolipoprotein E AN567-10M EP1373Y/Rabbit HK081-5KE

B cell AM158-10M MB2/Mouse None

B Lymphocyte antigen 36 (BLA.36) AM231-10M A27-42/Mouse None

BcI-2 Oncoprotein AM287-10M bcl-2/100/Mouse HK087-5KE

Bcl 2 AN541-10M E17/Rabbit HK521-XAK

Bcl 2 AM723-10M EP36/Rabbit HK058-5K

Bcl-6 AM708-10M LN22/Mouse HK058-5K

Bcl-x AN462-10M E18/Rabbit HK521-XAK

Beta-Catenin AN510-10M E247/Rabbit HK521-XAK

BOB-1 (New) AN731-10ME SP92/Rabbit HK522-XAK

Breast Cancer Antigen BCA 225 AM135-10M CU18/Mouse None

CA19-9 AM424-10M C241:5:1:4/Mouse HK081-5KE

CA 125 (Ovarian Tumor Marker) AM429-10M Ov185:1/Mouse HK058-5K

Caldesmon AN507-10M E89/Rabbit HK521-XAK

Calponin-1 AN502-10M EP798Y/Rabbit HK081-5KE

Calretinin AR413-10R Polyclonal/Rabbit HK081-5KE

Calretinin AM583-10M 2 E7/Mouse HK081-5KE, HK080-5K

Carcinoembryonic antigen (CEA) AM009-10M B01-94-11M-P/Mouse None

Carcinoembryonic antigen (CEA) AM365-10M CEA-88/Mouse None

Catenin (p120) AN523-10M YE372/Mouse HK521-XAK

Catenin Delta 1 (p120) AR706-10M Polyclonal/Rabbit HK080-5K

CD1a AM490-10M O10/Mouse HK521-XAK

CD3 Epsilon AN477-10M EP449E/Rabbit HK521-XAK

CD3 (T cell) AM322-10M PS1/Mouse HK058-5K

CD3 + CD20 AC559-10M EP449E+L26/Mouse & Rabbit HK521-XAK

CD4 AM421-10M 4B12/Mouse HK058-5K

CD4 (New) AN722-10M EP204/Rabbit HK057-5KE

CD4 + CD8 AC595-10M 4B12+EP1150Y/Mouse+Rabbit HK058-5K

CD5 AM430-10M 4C7/Mouse HK058-5K

CD5 AN512-10M EP2952/Rabbit HK522-XAK

CD7 AM702-10M LP15/Mouse HK080-5K

CD8 AM422-10M 1A5/Mouse HK058-5KE

CD8 AN513-10M EP1150Y/Rabbit HK081-5KE

CD8 (New) AN740-10M SP16/Rabbit HK081-5K

CD10 AM451-10M 56C6/Mouse HK521-XAK

i6000™ Automated Staining SystemProduct Name Cat. No.

i6000TM Automated Staining System AS6030 (Diagnostics), AS6040 (Infinity)

i6000TM Software 4812-00093 (Diagnostics), 4812-00094 (Infinity)

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Automated Systems

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CD10 + Cyclin D1 AC561-10M 56C6 + EPR2241/Mouse & Rabbit HK521-XAK

CD11b/ITAM AN546-10M EPR1344/Rabbit HK521-XAK

CD11c AN555-10M EP1347Y/Rabbit HK081-5KE

CD14 AN515-10M EPR3653/Rabbit HK521-XAK

CD15 (Blood group antigen Lewis X) AM302-10M BRA4F1/Mouse None


CD20 AM537-10M CD20/C23/Mouse HK081-5KE

CD20 (B cell) AM238-10M L-26/Mouse None

CD21 AN497-10M EP3093/Rabbit HK522-XAK

CD23 AR460-10R Polyclonal/Rabbit HK521-XAK

CD25 AM453-10M 4C9/Mouse HK521-XAK

CD30 (Ki-1 Antigen) AM327-10M Ber-H2/Mouse HK058-5K

CD30 (Ki-1 Antigen) AM351-10M HRS-4/Mouse HK081-5KE

CD31 AM232-10M JC/70A/Mouse EK000-10K

CD34 (Endothelial cell) AM236-10M QBend/10/Mouse None

CD35 AM431-10M RLB25/Mouse HK058-5K

CD35 (New) AN741-10M SP191/Rabbit HK522-XAK

CD41/Integrin Alpha IIb (New) AN732-10ME EP178/Rabbit HK057-5KE

CD45 (Leukocyte Common Antigen, LCA) AM111-10M PD7/26/16&2B11/Mouse None

CD45 cocktail (Leukocyte Common Antigen, LCA) AM371-10M MEM55-LJ 27.9/Mouse None

CD45RA (B cell) AM157-10M MB1/Mouse None

CD45RO (T cell) AM113-10M UCHL-1/Mouse None

CD48 (New) AN721-10ME EP148/Rabbit HK522-XAK


CD61 AN482-10M EP2417Y/Rabbit HK522-XAK


CD68 AM549-10M CD68/G2/Mouse HK522-XAK

CD68 AM416-10M KP1/Mouse HK081-5KE

CD79a AM414-10M 11E3/Mouse HK081-5KE

CD79a (New) AN719-10M EP82/Rabbit HK081-5KE


CD95 (New) AN742-10M EP208/Rabbit HK522-XAK

CD99 AN518-10M EPR3097Y/Rabbit HK521-XAK

CD99 AM355-10M HO36.1.1/Mouse HK058-5K


CD117 AM423-10M T595/Mouse HK081-5KE

CD117 AM465-10M YR145/Rabbit HK081-5KE


CD205 (New) AN737-10ME EP176/Rabbit HK081-5KE

CD227 (MUCIN 1) AM534-10M VU-4H5/Mouse HK521-XAK

CDX2 AM392-10M CDX2-88/Mouse HK081-5KE

CDX2 + CK7 AC596-10M CDX-2-88+EPR1619Y/Mouse+Rabbit HK081-5KE

CDX2 + CK20 + CK7 AC597-10M CDX-2-88+EPR1622Y+EPR1619Y/M+R HK081-5KE

c-erbB-2 (HER-2/neu) AN471-10ME EP1045Y/Rabbit None

c-erbB-2 (HER-2/neu) AM134-10ME CB11/Mouse None

c-erbB-2 (HER-2/neu) (New) AN726-10ME EP3/Rabbit HK081-5KE

Chromogranin A AM126-10M LK2H10/Mouse None

Chromogranin A AM356-10M PHE-5/Mouse HK058-5K

Claudin 1 AR704-10M Polyclonal/Rabbit HK080-5K

Claudin-5 (New) AN718-10M EP224/Rabbit HK081-5KE

Collagen IV AM379-10M COL-94/Mouse EK000-5K

Cyclin D1 AN474-10M EPR2241(IHC)-32/Rabbit HK521-XAK

i6000™ Reagents and Consumables

25

Automated Systems

[email protected]


Cyclin D1 AR447-10R Polyclonal/Rabbit HK081-5KE

Cyclin E2 AN519-10M EP454Y/Rabbit HK521-XAK

Cytokeratin 4 AM705-10M 6B10/Mouse HK087-5KE

Cytokeratin 4 (New) AN717-10M EP4/Rabbit HK081-5KE

Cytokeratin 5 AN484-10M EPR1600Y/Rabbit HK081-5KE

Cytokeratin 5 & 6 AN588-10M EPR1600Y & EPR1602Y/Rabbit HK081-5KE

Cytokeratin 5 & Cytokeratin 14 (New) AN730-10ME EP24/EP61/Rabbit HK081-5KE


Cytokeratin 7 AM255-10M OV-TL12/30/Mouse EK000-10K

Cytokeratin 7 AN528-10M EPR1619Y/Rabbit HK522-XAK

Cytokeratin 7 & 8 AM587-10M OV-TL12/30 & C51/Mouse HK081-5KE

Cytokeratin 8 AM142-10M C51/Mouse HK167-5K

Cytokeratin 8 & 18 AM131-10M 5D3/Mouse EK000-10K

Cytokeratin 14 AM146-10M LL002/Mouse HK167-5K

Cytokeratin 15 AN485-10M EPR1614Y/Rabbit HK521-XAK

Cytokeratin 16 AN486-10M EP1615Y/Rabbit HK521-XAK

Cytokeratin 17 AM572-10M E27/Mouse HK522-XAK

Cytokeratin 19 AM246-10M RCK108/Mouse EK000-10K

Cytokeratin 20 AM315-10M IT-Ks20.8/Mouse EK002-10K


Cytokeratin Cocktail AM071-10M AE1 and AE3/Mouse EK001-10K

Cytokeratin Cocktail, Broad spectrum AM372-10M LL002+DEK-10+RCK108+OVTL12/30+C11/Mouse EK000-10K

Cytokeratin Low MW AM075-10M AE1/Mouse EK000-10K

Cytokeratin, High MW AM291-10M 34ßE12/Mouse EK000-10K

Desmin AM072-10M D33/Mouse None

DOG 1 AM570-10M 1.1/Mouse HK081-5KE

E-Cadherin AM390-10M 36/Mouse HK058-5K

E-Cadherin (New) AN725-10M EP6/Rabbit HK081-5KE

EGFR (Pan) AN473-10ME EP38Y/Rabbit None

Ep-CAM AN489-10M E144/Rabbit HK087-5KE

Epithelial Membrane Antigen (EMA) AM057-10M E29/Mouse None

Epithelial Membrane Antigen (EMA) AM182-10M Mc5/Mouse None

Epithelial-Specific Antigen AM316-10M MOC-31/Mouse HK167-5K

Estrogen Receptor (ER Alpha) AN509-10ME EPR703(2)/Rabbit None

Estrogen Receptor AM272-10ME 1D5/Mouse HK081-5KE

Estrogen Receptor AR385-10R Polyclonal/Rabbit HK080-5K

Estrogen Receptor AN710-10ME EP1/Rabbit HK081-5KE

Estrogen Receptor (InSite® ER) AM368-10ME ER88/Mouse HK080-5K

Factor VIII-related antigen AM016-10M F8 2.2.9/Mouse EK000-10K

Factor XIIIa AN516-10M EP3372/Rabbit HK521-XAK

Factor XIII Subunit A AM337-10M E980.1/Mouse HK081-5KE

Fascin AM488-10M FCN01/Mouse HK087-5KE

Fibronectin AN569-10M F14/Rabbit HK081-5KE

Follicle Stimulating Hormone (FSH) AM026-10M 83/12/2A8 2C7/Mouse None

GCDFP-15 AN481-10M EP1582Y/Rabbit HK521-XAK

Glial Fibrillary Acidic Protein (GFAP) AM020-10M GA-5/Mouse None

Glut-1 AM505-10M SPM498/Mouse HK522-XAK

Glypican-3 AM539-10M GPC3-88/Mouse HK081-5KE

HSA AM550-10M HSA/E8/Mouse HK521-XAK


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Automated Systems

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Heat Shock Protein 27 (HSP 27) AM171-10M G3.1/Mouse HK081-5KE

Helicobacter pylori AR442-10RE Polyclonal/Rabbit None

HGH (Growth Hormone) AR707-10R Polyclonal/Rabbit HK521-XAK

IgM AR427-10R Polyclonal/Rabbit HK051-5KE

Inhibin Alpha AM446-10M R1/Mouse HK081-5KE

Insulin (New) AN735-10M EP125/Rabbit None

Kappa light chain AM048-10M L1C1/Mouse None

Kappa light chain AM369-10M K88/Mouse None

Ki-67 AM410-10M K2/Mouse HK081-5KE

Ki-67 AN527-10M EPR3611/Rabbit HK522-XAK

Ki-67 (New) AN727-10M EP5/Rabbit HK522-XAK

Ki-67 antigen, Proliferating cell AM297-10M MIB-1/Mouse HK087-5KE

Ki-67 antigen, Proliferating cell AM370-10M Ki88/Mouse HK081-5KE

Ki67+Lambda Light Chain AC562-10M K-2/Mouse + Polyclonal/Rabbit HK081-5KE

Lambda Light Chain (New) AN715-10M EP172/Rabbit HK058-5KE

Laminin AR078-10R Polyclonal/Rabbit EK000-5K

Lysozyme AR024-10R Polyclonal/Rabbit HK521-XAK

Mast Cell Tryptase AM419-10M AA1/Mouse None

Melan-A (MART-1) AM361-10M A103/Mouse HK087-5KE

Melanoma AM001-10M HMB45/Mouse HK081-5KE

Melanoma-Associated Antigen AM077-10M NKI/C3/Mouse None

Melanoma gp100 AM536-10M gp100/D5/Mouse HK087-5KE

Mesothelin AM433-10M 5B2/Mouse HK058-5K

MiTF AM554-10M MiTF/A13/Mouse HK522-XAK

MLH1 AM703-10M ES05/Mouse HK081-5KE

MMP9 AN504-10M EP1255Y/Rabbit HK522-XAK

MSH2 (New) AN743-10M SP46/Rabbit HK521-XAK

MSH6 AM454-10M 2D4B5/Mouse HK521-XAK

MUC4 AM455-10M 1G8/Mouse HK521-XAK

MUC5AC AM456-10M 45M1/Mouse HK521-XAK

Mucin 1 (MUC1) AN564-10M EPR1023/Rabbit HK081-5KE

Myelin Basic Protein AM380-10M MBP88/Mouse None

Myeloperoxidase (MPO) AR496-10R Polyclonal/Rabbit HK522-XAK

Myf4 AM432-10M LO26/Mouse HK522-XAK

Myosin Heavy Chains, Smooth Muscle AM331-10M SMMS.1/Mouse HK058-5K

N-Cadherin AN565-10M EPR1792Y/Rabbit HK081-5KE

Napsin A AM701-10M IP64/Mouse HK521-XAK

Neurofilament AM073-10M NE-14/Mouse None

Neuron Specific Enolase (NSE) AM055-10M MIG-N3/Mouse None

NGFR AN522-10M EP1039Y/Rabbit HK522-XAK

NGFR (New) AN738-10M EP31/Rabbit HK081-5KE

Oct 2 AN558-10M EPR542/Rabbit HK081-5KE

Oct 4 (New) AN724-10M EP143/Rabbit HK081-5KE

p16 [INK4a] AM540-10M G175-405/Mouse HK081-5KE

p16 + KI67 AC601-10M G175-405+EPR3611/Mouse+Rabbit HK081-5KE

p27 (Kip1) AN492-10M Y236/Rabbit HK521-XAK

P504S (AMACR) AN538-10M RBT-AMACR/Rabbit HK521-XAK

P504S (AMACR) AN449-10ME 13H4/Rabbit HK081-5KE

p53 (New) AN728-10M EP9/Rabbit HK522-XAK

p53 Protein AM195-10M BP53-12-1/Mouse HK087-5KE


27

Automated Systems

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p53 Protein AM239-10M DO7/Mouse HK087-5KE

p53 Protein AM240-10M 1801/Mouse HK087-5KE

p63 AM418-10M 4A4/Mouse HK081-5KE

p63+CK5 AC602-10M 4A4/Mouse + EPR1600Y/Rabbit HK081-5KE

PAP AM532-10M A40010/Mouse HK521-XAK

PAX5 AM457-10M ZP007/Mouse HK521-XAK

Paxillin (N-Term) AN521-10M Y113/Rabbit HK521-XAK

PDCD4 AN524-10M EPR3432/Rabbit HK521-XAK

PDGF Receptor-β AN463-10M Y92/Rabbit HK521-XAK

PGP9.5 (New) AM736-10ME 3D9/Mouse HK081-5K

PIN4 (p63 + CK HMW + P504S) AM448-10ME 4A4/Mouse+34BE12/Mouse+13H4/Rabbit HK081-5KE

Placental Alkaline Phosphatase (PLAP) AM228-10M PL8-F6/Mouse None

Placental Lactogen (hPL) AR040-10R Polyclonal/Rabbit None

PMS2 AN577-10M EPR3947/Rabbit HK081-5KE

Progesterone receptor AM172-10ME 1A6/Mouse None

Progesterone receptor AN711-10ME EP2/Rabbit HK087-5KE

Progesterone receptor (InSite® PR) AM328-10ME PR88/Mouse HK087-5KE

Prolactin AM031-10M ME-121/Mouse None

Proliferating Cell Nuclear Antigen (PCNA) AM252-10M PC10/Mouse HK087-5KE

Prostate specific acid phosphatase (PSAP) AM013-10ME B01-94-21M-NA/Mouse None

Prostate specific antigen (PSA) AM014-10ME ErPr-8/Mouse None

pS2 Estrogen Inducible Protein AM190-10M PS2.1/Mouse EK002-5K

PSMA (New) AN714-10M EP192/Rabbit HK081-5KE

RCC (Renal Cell Carcinoma) AM543-10M RCC-26/Mouse HK081-5KE

S100 beta (New) AN713-10M EP32/Rabbit HK087-5KE

S-100 Protein AR058-10R Polyclonal/Rabbit None

S-100 Protein AM058-10M 15E2E2/Mouse None

S100P (New) AN712-10M EP186/Rabbit HK087-5KE

Sarcomeric Actin AM511-10M ZMSA-5/Mouse HK521-XAK

Stat 5-Alpha (C-term) AN480-10M E289/Rabbit HK521-XAK

Survivin AN503-10M EP2880Y/Rabbit HK521-XAK

Synaptophysin AM363-10M Snp88/Mouse HK081-5KE

Tau AM412-10M Tau-2/Mouse HK081-5KE

Tau AM459-10M Tau-5/Mouse HK521-XAK

Terminal Deoxynucleotidyl Transferase (TdT) AM373-10M TdT/Mouse HK087-5KE

TFPU.1 AN520-10M EPR3159Y/Rabbit HK521-XAK

Thyroglobulin AM032-10M 2H11/Mouse None

Thyroid Transcription Factor (TTF-1) AM397-10M 8G7G3/1/Mouse HK081-5KE

TIA-1 AM529-10M 2G9A10F5/Mouse HK521-XAK

Topo 2 Alpha AN576-10M EP1102Y/Rabbit HK081-5KE

Thyroid Stimulating Hormone (TSH) AM033-10M 5404/Mouse None

TTF1+CK5/6 AC563-10M 8G7G3/1/Mouse+EPR1600Y&EPR1602Y/Rabbit HK080-5K

TTF1+CK20+CK7 AC605-10M 8G7G3/1+EP1582Y+EPR1619Y/Mouse+Rabbit HK081-5KE

TTF1 + GCDFP-15 AC604-10M 8G7G3/1+EP1582Y/Mouse+Rabbit HK087-5KE

Tumor-Associated Glycoprotein (TAG-72) AM054-10M B72.3/Mouse None

Tyrosinase AM535-10M Ty/G5/Mouse HK521-XAK

VEGF AR483-10R Polyclonal/Rabbit HK521-XAK

Villin AM525-10M ID2C3/Mouse HK522-XAK

Vimentin AM074-10M V9/Mouse None

VIP AR530-10R Polyclonal/Rabbit HK522-XAK

Wilm’s Tumor AN584-10M CAN-R9(IHC)-56-2/Rabbit HK081-5KE

ZAP-70 AM544-10M ZAP70-C3/Mouse HK521-XAK

ZAP-70 AN466-10M YE291/Rabbit HK521-XAK


28

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The OptiMiser reagent vials (U.S. & Foreign Equivalent Patents Pending) are available as a 20 ml disposable barcoded pack for use on the i6000TM and the OptiMax staining systems.

Note: Unlabeled Vials - for open system only


OptiMiser Reagent Vials, Labeled (20 ml) (Empty Vials supplied with 100 corresponding slide barcode labels)

1 each XT026-601 to XT026-799 XT026-601P to XT026-660P

OptiMiser Reagent Vials, Unlabeled (20 ml) White Pack of 24 XT026-V24

OptiMiser Reagent Vials, Unlabeled (20 ml) Brown Pack of 24 XT101-24X

OptiMiser Universal Vial Holders Pack of 24 XT027-H24

OptiMiser Vial Caps Pack of 24 XT022-CP

Empty Reagent Vials for ISH Probes 1 each XT026-PR601 to XT026-PR615


Super Sensitive™ One-step Polymer-HRP Detection Kit/DAB 200 slides QD610-YAXE

Super Sensitive™ Polymer HRP Detection System/DAB 200 slides QD410-YAXE

Avidin/Biotin Blocking Kit RTU 200 slides HK102-20X

Avidin/Biotin Blocking Kit RTU 100 slides HK102-10X

Multilink detection kitHRP/DAB (2 components) 200 slides QD470-YCX

Our all-inclusive, Super SensitiveTM Detection Systems contain all the reagents required for easy, fast, and exceptional staining. Each kit contains enough reagents to stain approximately 200 slides at 100 µl per slide. The following kit configurations are available to fit the laboratory’s needs for any staining requirement. Reagents are offered in barcoded vials designed for use on the i6000TM and the OptiMax

Staining Systems.

Detection Systems

OptiMiser Reagent Vials and Accessories (User Defined)


Note: Please enquire for Double Stain Detection Kits

29

Automated Systems

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Barrier Slides, PAP Pen, and Barcode LabelsOptiPlusTM Positively-charged Barrier Slides (U.S. & Foreign Equivalent Patents Pending) contain hydrophobic barriers that allow the quantity of reagent per slide to be tailored to the size of the specimen. These slides come in three configurations to accommodate different tissue sizes or multiple tissues per slide; A single full-size test area of 25 mm x 40 mm, a single 2/3-size test area of 25 mm x 30 mm, and three 1/3-size test areas per slide, each measuring 25 mm x 15 mm. The permanent hydrophobic barriers are compatible with dewaxing solutions and other reagents. The slides are suitable for use with frozen tissue sections, formalin-fixed paraffin sections, and cytology preparations.

Pipette Tips Each pipette tip is carefully inspected to ensure optimal and accurate performance.


Pipette Tips for i6000™ (1.0 ml) 1 box (192 tips) XT105-01X

Pipette Tips for i6000™ (1.0 ml) 5 boxes (960 tips) XT104-05X

EZ-DeWax™ Solutions1

Tissue specimens are usually fixed and embedded in paraffin, sectioned on a microtome, and then attached to slides. Before immunostaining, the sections are traditionally deparaffinized with highly toxic, noxious chemicals (xylene and alcohols or equivalents). BioGenex offers a revolutionary product that simply, easily and rapidly removes the paraffin from mounted tissue slides. Use of non-xylene based BioGenex EZ-DeWaxTM Solution permits a two-step application of a single reagent that completely removes the paraffin, rendering the tissue’s antigenic sites accessible to the antibodies, chromogens and other aqueous solutions. The deparaffinization time is reduced from 45 minutes of manual processing to less than 15 minutes of automated dewaxing on the BioGenex i6000TM Automated Staining System using the EZ-DeWaxTM Solution. The solution simultaneously removes paraffin and rehydrates the tissue.

Ancillary Reagents

1 US Patent No. 6,632,598; Japanese Patent No. 3532571; European Patent No. 0698118B1.


EZ-DeWax™ Solution (Concentrated)1

(Requires 500 ml of histologic grade alcohol for reconstitution)500 ml HK584-5KE


OptiPlus™ Positively-charged Barrier Slides (full test area) 1 box (72 slides) XT134-SL

1 case (20 boxes) XT134-CL

OptiPlus™ Positively-charged Barrier Slides (2/3 test area) 1 box (72 slides) XT013-SL


OptiPlus™ Positively-charged Barrier Slides (3 x 1/3 test area) 1 box (72 slides) XT014-SL


PAP Pen (for 500 - 1000 slides) 1 each XT001-PP

Slide Barcode Labels 100/sheet AM6010 to AM7990 AR6010 to AR6600


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Automated Systems

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Wash Buffers Super SensitiveTM Wash Buffers are used to ensure optimal staining with even spreading of antibodies and other reagents to avoid in-consistent results.


Super Sensitive™ Wash Buffer, 20X concentrated 500 ml HK583-5KE

Enzymes for Pre-treatment


Pepsin 4-Pack 4 vials of Lyophilized Enzyme Powder, Reconstitution Buffer 4 x 5 ml

200 slides EK000-10K

Trypsin 4-Pack 4 vials of Lyophilized Enzyme Powder, Reconstitution Buffer 4 x 5 ml


Protease XXIV 4-Pack 4 vials of Lyophilized Enzyme Powder, Reconstitution Buffer 4 x 5 ml


Diastase (Alpha-Amylase Kit) 4 vials of alpha-amylase, 4 vials of alpha-amylase diluent

200 slides EK004-5K

Some tissues require the use of enzymatic pre-treatment before staining to achieve standardized results depending on the antibodies and their different incubation and pre-treatment requirements.


EZ-AR™ 1 RTU1 1L EZ-AR™ 1 is a Citra based solution. Works at 107°C HK521-XAK

EZ-AR™ 2 RTU1 1L EZ-AR™ 2 is a EDTA based solution. Works at 107°C HK522-XAK

EZ-AR™ 3 Conc.1 (10X) 500 ml EZ-AR™ 3 is a Citra based solution. Works at 107°C HK543-YOK

EZ-AR™ 4 Conc.1 (10X) 500 ml EZ-AR™ 4 is a Tris based solution. Works at 107°C HK544-YOK

EZ-AR™ Common, Conc.1 (5X) 1 L DeWax solution. Use in combination with other EZ-AR™ solutions HK545-XOK

1 U.S. Patent Numbers 6,451,551 and 5,578,452 (as well as foreign equivalents)

EZ-AR™ Solutions

Instrument Accessories


i500 Plus™ LIS Enabled Barcode Label Printer BLS500


Resin Ribbon 1 Roll XT034-XEX

Labels Roll 1 Roll XT035-XBX


Tissue Pre-treatment & Antigen Retrieval

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EZ-DeWax™ Solutions

BioGenex EZ-DeWaxTM Solution is a “one-step” product that simultaneously enables the removal of paraffin and allows rehydration of the tissue with a single reagent. In the past, formalin-fixed, paraffin-embedded tissue sections were traditionally deparaffinized with highly toxic, noxious chemicals (i.e. xylene, equivalents). BioGenex, a pioneer in the Immunohistochemistry technology, offers a xylene-free product that removes the paraffin from mounted tissue slides easily and rapidly.

Advantages of EZ-DeWaxTM Solutions • Effectively removes paraffin and other wax residues from tissue sections• Avoids the use of xylene or xylene substitutes• Reduces the use of alcohol in preparing tissue sections for IHC, ISH, H & E, FISH and Special Stains• Has no adverse effect on the quality of tissue sections • Reduces deparaffinization time from 45 minutes to 10 minutes• Produces minimal or no odors• Reduces the quantity of toxic solvents used in the lab• Minimizes hazardous waste and easy to dispose• Optimized for all BioGenex Automated Staining Systems and manual methods

† U.S. Patent No. 6,632,598; U.S. Patent No. 7, 070, 951; Japanese Patent No. 3532571; European Patent No. 0698118B1

.


EZ-DeWax™ Solution (Concentrated) 500 ml HK584-5KE†

One-Step DeWaxing and Rehydration Reagent

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BioGenex is the inventor of Antigen Retrievala enabling technology. Antigen Retrieval is an effective way of unmasking antigenic epitopes on the surface of formalin-fixed, parraffin-embedded tissue sections using microwave heating. Covered by exclusive patents1 issued to BioGenex, this method to recover antigenicity in formalin-fixed, paraffin-embedded tissue sections has been routinely practiced in laboratories throughout the world.

The Antigen Retrieval technique breaks the formalin induced cross-linking bonds between epitopes and unrelated proteins, thereby allowing better penetration of antibody and accessibility of epitopes.

Advantages of the Antigen Retrieval Technique• Enhanced exposure of antigenic epitopes on the surface of the tissue section• Reduces time for primary antibodies incubation• Consistent and reliable staining quality• Eliminates false negative staining results in FFPE tissue sections• Ease-of-use

Different antibodies require different conditions for Antigen Retrieval.

BioGenex offers several types of Antigen Retrieval solutions: 1. EZ-ARTM Solutions2. Citra3. Citra Plus4. AR-10† 5. H&E Retrieval6. DeCal

Antigen Retrieval Method

a Shi S-R, Key ME, Kalra KL. Antigen retrieval in formalin-fixed, paraffin-embedded tissues: an enhancement method for immunohistochemical staining based on microwave oven heating of tissue sections. J Histochem Cytochem 39:741-8, 1991.

1 U. S. Patent Numbers 5,244,787; 5,578,452 and 6,451,551; Japanese Patent No. 3108099; European Patent No. 0607422.

Tonsil tissue stained with anti-Ki-67 antibody using AEC chromogen without antigen retrieval

Tonsil tissue stained with anti-Ki-67 antibody using AEC chromogen with antigen retrieval

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EZ-AR™ Elegance Solutions The EZ-AR™ Elegance Solutions are designed to enable optimal dewaxing, rehydration and antigen retrieval on FFPE sections. The solutions can reach temperature of 107°C without boiling thus preventing reagent evaporation. When used with EZ-Retriever® System or Xmatrx® Automated Staining System, these solutions ensure superior reproducible results with uncompromised staining quality.

Features and Benefits

Works at 107°C without boiling • Minimizes reagent evaporation • Enhances exposure of antigenic epitopes

Eliminates enzymatic pre-treatment • Reduces background • Preserves tissue morphology

Reusable up to three times • Reduces material cost

Non-hazardous, non-flammable and odorless • Safe and eco-friendly

Product Name Pack Size Product Description Cat. No.

EZ-AR™ 1 Elegance RTU 1L EZ-AR™ 1 Elegance is Citra based solution. Works at 107°C HK546-XAK

EZ-AR™ 2 Elegance RTU 1L EZ-AR™ 2 Elegance is EDTA based solution. Works at 107°C HK547-XAK

For EZ-AR™ Elegance Solutions compatible with Xmatrx® Automated Staining System, refer to Automation Section

The Antigen Retrieval Accessory Kit consists of slide holders and slide baths that make it convenient and compatible with any of the several Antigen Retrieval solutions. To accommodate microwave heating, the slide baths and slide holders are made of heat-stable thermoplastic polyolefin and hydrocarbon polymers of acetal resins. These accessories may be used in a microwave or a pressure cooker.

Antigen Retrieval Accessory KitsProduct Name* Pack size Cat. No.

3 Slide Baths with Lids 3 Slide Holders (72-slide capacity)

1 Kit MW001-HB

1 Slide Bath with Lid 1 Slide Holder (24-slide capacity)

1 Kit MW001-SU

Antigen Retrieval Accessory Kit

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The EZ-ARTM Solutions possess unique properties that enable optimal dewaxing, rehydration and antigen retrieval in formalin-fixed, paraffin-embedded tissue sections. Some solutions can reach temperatures as high as 95oC. These solutions when used with EZ-Retriever® System facilitate the production of highly reproducible and superior quality stains in a considerably short time without compromising the morphology and antigenicity of the tissue.

Features and Benefits

Unique superheating properties • Increases the availability of antigenic epitopes in tissues

Eliminates enzymatic pre-treatment of tissue sections • Reduces background staining • Preserves tissue morphology

Streamlined set of solutions • Offers a simple solution to antigen retrieval pre-treatment needs

Short, simple, and standardized protocols for all BioGenex antibodies • Reduces tissue pre-treatment time • Eliminates guesswork in optimizing protocol

Fast uniform heating and cooling of solutions • Reduces tissue pre-treatment time

Non-hazardous, non-flammable and odorless • Safe and Eco-friendly

Product Name Pack Size Product Description Cat. No.

EZ-AR™ 1 RTU 1L EZ-AR™ 1 is a Citra based solution. Works at 95°C HK521-XAK

EZ-AR™ 2 RTU 1L EZ-AR™ 2 is a EDTA based solution. Works at 95°C HK522-XAK

EZ-AR™ Common, Conc. (5X) 1 L DeWax solution. Use in combination with other EZ-AR™ solutions HK545-XOK

EZ-AR™ 3 Conc. (10X) 500 ml EZ-AR™ 3 is a Citra based solution. Works at 95°C HK543-YOK

EZ-AR™ 4 Conc. (10X) 500 ml EZ-AR™ 4 is a Tris based solution. Works at 95°C HK544-YOK

For Laboratory Use Only

EZ-AR™ Solutions

Enable Highly Reproducible and Superior Quality Stains

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www.biogenex.com

Antigen Retrieval Solutions Citra† Solutions This citrate-based solution provides excellent staining of routine paraffin sections with most BioGenex antibodies, including Progesterone Receptor (PR), p53 protein, and Ki-67 Antigen (clone Ki88) when used in a microwave.

• Retrieves most antigens in formalin-fixed, paraffin-embedded tissue sections • Excellent results with difficult antigens like PR, p53 and Ki-67 Antigen

Citra Plus† SolutionsThis product is an enhanced version of our standard Antigen Retrieval Citra Solution for use in microwave and is designed for immunostaining with Estrogen Receptor (ER), HSP27, and certain other antibodies as recommended by BioGenex.

• Retrieves most antigens in formalin-fixed, paraffin-embedded tissues • Excellent results with ER Antigen

† U.S. Patent No. 5,578,452; Japanese Patent No. 3108099; European Patent No. 0607422.


Antigen Retrieval Citra Solution (10X Concentrated) 100 ml HK086-5K

Antigen Retrieval Citra Solution (10X Concentrated) 500 ml HK086-9K

Antigen Retrieval Citra Solution (Ready-to-use) 250 ml HK087-5KE

Antigen Retrieval Citra Solution (Ready-to-use) 1000 ml HK087-20KE


Antigen Retrieval Citra Plus Solution (10X Concentrated) 100 ml HK080-5K

Antigen Retrieval Citra Plus Solution (10X Concentrated) 500 ml HK080-9K

Antigen Retrieval Citra Plus Solution (Ready-to-use) 250 ml HK081-5KE

Antigen Retrieval Citra Plus Solution (Ready-to-use) 1000 ml HK081-20KE

Breast Carcinoma tissue stained with Anti-Progesterone Receptor [PR88] MAb,

using AEC chromogen without Antigen Retrieval.

Breast Carcinoma tissue stained with Anti-Estrogen Receptor [ER88] MAb, using DAB chromogen without Antigen Retrieval.

Breast Carcinoma tissue stained with Anti-Progesterone Receptor [PR88]

MAb, using AEC chromogen with Antigen Retrieval using Citra.

Breast Carcinoma tissue stained with Anti-Estrogen Receptor [ER88] MAb, using DAB chromogen with Antigen

Retrieval using Citra Plus.

Please Contact Technical Support for a complete list of recommended antibodies to be used with Antigen Retrieval Citra Solution.

Please Contact Technical Support for a complete list of recommended antibodies to be used with Antigen Retrieval Citra Plus† Solution.

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AR-10† Solutions Antigen Retrieval AR-10† is a Tris-based solution that produces excellent immunostaining with BioGenex antibodies like Caldesmon, CD3 (Clone PS1), c-myc Protein, PECAM (Clone 9G11), GLEPP1, and other antigens in formalin-fixed, paraffin embedded tissue sections when used in a microwave.


Antigen Retrieval AR-10 Solution (10X Concentrated) 100 ml HK057-5KE

Antigen Retrieval AR-10 Solution (Ready-to-use) 250 ml HK058-5K

Antigen Retrieval AR-10 Solution (Ready-to-use) 1000 ml HK058-20K

Please Contact Technical Support for a complete list of recommended antibodies to be used with Antigen Retrieval AR-10† Solution.

†U.S. Patent No. 5,578,452; Japanese Patent No. 3108099; European Patent No. 0607422.

H&E Retrieval SolutionsThe H&E Retrieval Solution will help recover nuclear staining in burnt or overfixed formalin-fixed, paraffin-embedded tissue sectionsa .

• Proven to restore nuclear staining detail for improved hematoxylin staining in burnt tissues and fixed or overfixed formalin-fixed, paraffin-embedded tissue sections

• Highly recommended pre-treatment step for specimens dried out before fixing in formalin, over-DeCal specimens (e.g. bone marrow biopsies) and over-processed specimens (i.e. fragile specimens such as needle biopsies)

• H&E Retrieval solution can be used at room temperature for some frozen tissue sections & specimens that may contain freezing artifacts


H&E Retrieval Solution (10X Concentrated) 100 ml HK169-5K

Tonsil tissue stained with anti-CD3 MAb using DAB chromogen without

Antigen Retrieval using AR-10†.

Tonsil tissue stained with anti-CD3 MAb using DAB chromogen with Antigen

Retrieval using AR-10†.

Nuclear data restored by microwave heating in H&E Solution.

Burnt kidney tissue stained afterstandard pre-treatment.

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Enzymes for Tissue DigestionSome tissues require the use of enzymatic pre-treatment before staining to achieve standardized results depending on the antibodies and their different incubation and pre-treatment requirements.

Each kit contains three vials of lyophilized enzyme powder and 15 ml of reconstitution buffer, enabling you to make fresh enzyme solutions as needed.

The Trypsin 3-Pack and Pepsin 3-Pack contain well-established enzymes suitable for routine pre-treatment at 37°C.

The Protease XXIV 3-Pack contains a universal digestive agent that allows for fast and effective pre-treatment at room temperature.


Pepsin 3-Pack 150 slides EK000-5K 3 vials of lyophilized enzyme powder, 3 x 5 ml reconstitution buffer

Trypsin 3-Pack 150 slides EK001-5K 3 vials of lyophilized enzyme powder, 3 x 5 ml reconstitution buffer

Protease XXIV 3-Pack 150 slides EK002-5K 3 vials of lyophilized enzyme powder, 3 x 5 ml reconstitution buffer

Diastase (Alpha-Amylase Kit) 200 slides EK004-5K 4 vials of alpha-amylase, 4 vials of alpha-amylase diluent

Enzymes for Tissue Digestion

Colonic adeno carcinoma stained with Anti-Cytokeratin 20 MAb following Protease

Pre-treatment.

Leiomyoma tissue stained with Factor VIII MAb using AEC chromogen following Pepsin

Pre-treatment.

Squamous Carcinoma tissue stained with Anti-Cytokeratin (High Molecular Weight) MAb using AEC chromogen following Trypsin Pre-treatment.

With the new paradigm in Antigen Retrieval using the EZ-Retriever® System, enzymatic pre-treatment can be eliminated from the protocol. For more information on the EZ-Retriever® System please refer to the Automation section.

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Placing the NordicWare® Microwave Tender Cookera within a microwave is an effective method for enhancing staining with the Antigen Retrieval technique. The heat produced under enhanced pressure can reduce the build up of gas bubbles on the surface of tissues. This improves the intensity of staining, accompanied by preservation of tissue and cell morphology. This pressure cooker is also optimized for use with various BioGenex Antigen Retrieval solutions.

NordicWare® Microwave Tender Cooker

For Laboratory Use onlya NordicWare® is a registered trademark of NordicWare Corp.


NordicWare® Microwave Tender Cooker 1 Unit NW001-PC

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www.biogenex.com

Primary Antibodies

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Primary Antibodies

www.biogenex.com

BioGenex antibodies are optimized to provide maximum signal with minimum background when used for immunohistochemical staining.

FormatReady-to-use antibodies are fully optimized for use with BioGenex detection systems without the need for further dilution or titration. Ready-to-use, Super SensitiveTM antibodies are fully quality controlled. These antibodies are recommended for use with all Super SensitiveTM Detection Systems to provide optimum staining. The ready-to-use antibody formats are indicated by catalog numbers prefixed with AC (Antibody Cocktails), AM (Mouse Monoclonal Antibodies), AN (Rabbit Monoclonal Antibodies), AY&AX (Monoclonal Antibodies for Xmatrx®), AW (Polyclonal Antibodies for Xmatrx® ) and AR (Polyclonal Antibodies).

Concentrated antibodies are provided with recommended dilutions for optimal use with BioGenex detection systems, allowing rapid titration and testing. These provide a more economical alternative for laboratories doing high volume immunostaining. The concentrated antibody formats are indicated by catalog numbers prefixed with MU (Mouse Monoclonal Antibodies), NU (Rabbit Monoclonal Antibodies) and PU (Polyclonal Antibodies).

All BioGenex concentrated antibodies are thoroughly tested for immunostaining applications and come with recommended dilutions for use with BioGenex detection systems. For specific information on individual antibody titers, please call BioGenex Technical Support at 1(800)421-4149 or write to: [email protected].

Pack SizeUnless otherwise specified, the following table lists the pack size for the available formats of antibodies:

Description Pack size Order information in Cat. No.

Ready-to-use (Manual) 3 ml 6 ml

2M and 2ME -5M, 5ME, 5R and 5RE

Ready-to-use (i6000TM) 10 ml 10M, 10ME, 10R and 10RE

Ready-to-use (Xmatrx®) 16 ml -YCD and YCDE

Concentrated 1 ml UC, UCE, UP and UPE

Tissue TypeUnless otherwise noted, all primary antibodies are optimized for use on routine formalin-fixed paraffin-embedded tissue.

OptimizationAll BioGenex primary antibodies are quality controlled and tested to provide optimum immunohistochemical staining when used with the appropriate BioGenex detection system. The correct optimization of antibody and detection system minimizes the potential for false negative or false positive staining.

Recommended Pre-treatmentThe recommended pre-treatment for each antibody is provided under each description of the antibody.

BioGenex offers EZ-Retriever® System for Dewaxing, Rehydration and Antigen Retrieval, that streamlines and simplifies tissue pre-treatment. For more details on the system please refer to Automated Systems section.

We recommend that you refer to the datasheet (i.e. package insert) provided with the antibodies for up-to-date information on the pre-treatment conditions or please contact BioGenex Technical Support at 1(800)421-4149 or write to: [email protected].

Positive Tissue Control Slides And Barrier SlidesBioGenex provides positive tissue control for use with the antibodies. The appropriate catalog number for the positive control slides with and without barrier are provided. For further details, refer to the Tissue Control section.

Antibody Look-Up TableThe table titled as “Antibody Look-Up Table” in the beginning of this section provides comprehensive information on all BioGenex primary antibodies along with positive controls.

IVD ProductsUnless specified otherwise, all Primary Antibodies listed in this Section are for In Vitro Diagnostic Use.

Primary Antibodies

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Primary Antibodies

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Antibody Look-up Table

Please consult the data sheet for pre-treatment and protocol information. Unless specified otherwise, all primary antibodies listed in this table are for FFPE tissue specimens‡ M: Mouse; R:Rabbit

Antibody Clone (Species) ‡ Localization Positive Control Regulatory Classification

Page

Aberrant Endothelial Cell 4A11(M) Cyt Tonsil / Lymph Node IVD 51

ACTH AH26(M) Cyt Pituitary IVD 51

Actin, Muscle-Specific HHF35(M) Cyt Heart / Skeleton Muscle IVD 51

Actin, Smooth Muscle 1A4(M) Cyt Colon IVD 51

Adenovirus A62020069P(M) Nuc and Cyt (Frozen) Adenovirus infected cells IVD 52

Alpha-1-Antichymotrypsin α1A88(M) Cyt Liver carcinoma IVD 52

Alpha-1-Antitrypsin Polyclonal(R) Cyt Hepatocellular carcinoma IVD 52

Alpha-Actinin JLN20(M) Cyt Muscle IVD 52

Alpha-Fetoprotein (AFP) C3(M) Cyt Hepatocellular carcinoma IVD 53

Alpha-Tubulin DM-1A(M) Cyt Skin/Lung IVD 53

Androgen Receptor F39.4.1(M) Nuc Prostate/Prostate Ca. IVD 53

Apolipoprotein E EP1373Y(R) Mem and Cyt Liver IVD 53

B Cell MB2(M) Cyt Tonsil IVD 54

B Lymphocyte Antigen 36 (BLA.36) A27-42(M) Mem B Lyphocytes/Hodgkin IVD 54

Bax Protein Polyclonal(R) Cyt and Mem Breast Carcinoma IVD 54

Bcl-2 E17(R) Cyt Follicular and lymphoma IVD 54

Bcl-2 EP36(R) Cyt Tonsil IVD 55

Bcl-2 Oncoprotein Bcl-2/100(M) Cyt Tonsil IVD 55

Bcl-6 LN22(M) Nuc Tonsil IVD 55

Bcl-x E18(R) Mem Breast Carcinoma IVD 55

Beta-Catenin E247(R) Mem/Cyt/Nuc Colon Carcinoma IVD 56

Beta-Tubulin DM-1B(M) Cyt Skin/Lung IVD 56

Beta-Tubulin II JDR3B8(M) Cyt Colon IVD 56

Beta-Tubulin III SDL3D10(M) Cyt Heart IVD 56

Beta-Tubulin IV ONS1A6(M) Cyt Lung IVD 57

bFGF (Basic Fibroblast Growth Factor) bFGF88(M) Cyt Adenocarcinoma IVD 57

Blood Group Antigen Lewis A 7LE(M) Cyt and Mem Stomach IVD 57

Blood Group Antigen Lewis B 2-25LE(M) Cyt and Mem Stomach IVD 57

BOB-1 SP92(R) Nuc Tonsil IVD 58

BRCA1 Protein Polyclonal(R) Nuc and Cyt Breast Carcinoma IVD 58

Breast Cancer Antigen BCA 225 CU18(M) Cyt Breast Carcinoma IVD 58

CA 19-9 C241:5:1:4(M) Cyt Colon IVD 58

CA 125 (Ovarian Tumor Marker) Ov185:1(M) Mem/Cyt Ovary Carcinoma IVD 59

Caldesmon E89(R) Cyt Prostate IVD 59

Caldesmon,High MW,Smooth muscle h-CD(M) Cyt Breast Carcinoma IVD 59

Calponin CALP(M) Cyt Breast Carcinoma IVD 59

Calponin-1 (C-term) EP798Y(R) Cyt Pleomorphic Adenoma IVD 60

Calretinin 2 E7(M) Cyt Brain Tissue IVD 60

Calretinin Polyclonal(R) Cyt Brain IVD 60

Carcinoembryonic Antigen (CEA) B01-94-11M-P(M) Cyt Normal Colon/ Colon Carcinoma IVD 60

Carcinoembryonic Antigen (CEA) CEA88(M) Cyt Bowel Carcinoma IVD 61

Carcinoembryonic Antigen (CEA) Polyclonal(R) Cyt Bowel/Colon Carcinoma IVD 61

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Page

Catenin (p120) YE372(R) Cyt/Mem Breast Carcinoma IVD 61

Catenin Delta 1 (p120) Polyclonal(R) Cyt and Mem Breast Carcinoma IVD 61

Cathepsin D C15(M) Cyt Breast Carcinoma IVD 62

CD1a O10(M) Mem Lymph Node IVD 62

CD2 AB75(M) Mem Lymphoma IVD 62

CD3 Epsilon EP449E(R) Mem Spleen IVD 62

CD3 (T Cell) UCHT1(M) Mem(Frozen) Tonsil IVD 63

CD3 (T Cell) PS1(M) Mem Tonsil IVD 63

CD4 4B12(M) Mem Tonsil IVD 63

CD4 EP204(R) Mem Tonsil/T-cell lymphoma IVD 63

CD5 4C7(M) Mem Tonsil IVD 64

CD5 EP2952(R) Mem Lymph Node IVD 64

CD7 LP15(M) Mem Tonsil IVD 64

CD8 1A5(M) Mem Tonsil IVD 64

CD8 SP16(R) Mem Tonsil IVD 65

CD8 (C-Term) EP1150Y(R) Membrane Tonsil IVD 65

CD8 (T Cell,Suppressor/Cytotoxic) T8(M) Mem(Frozen) Tonsil IVD 65

CD10 56C6(M) Mem Lymphoma IVD 65

CD11b (C3bi Receptor) M01(M) Mem(Frozen) Tonsil IVD 66

CD11b/ITAM EPR1344(R) Mem Spleen IVD 66

CD11C EP1347Y(R) Mem Tonsil Tissue IVD 66

CD14 EPR3653(R) Mem Placenta IVD 66

CD15 (Blood group antigen Lewis X) BRA4F1(M)Mem/Cyt/perinuclear structures

Bone Marrow/Hodgkins IVD 67

CD16 2H7(M) Mem and Cyt Lymph Node/Breast IVD 67

CD19 EP169(R) Mem Tonsil IVD 67

CD20 CD20/C23(M) Membrane Spleen/Tonsil IVD 67

CD20 (B Cell) L-26(M) Mem Tonsil IVD 68

CD20 (B Cell) B1(M) Mem(Frozen) Tonsil IVD 68


CD21 (B Cell) B2(M) Mem(Frozen) Tonsil/Lymph Node IVD 68

CD22 FPC1(M) Mem Tonsil IVD 69

CD23 Polyclonal(R) Mem Tonsil IVD 69

CD25 4C9(M) Mem Tonsil IVD 69

CD29 JB1a(M) Mem Breast IVD 69

CD30 (Ki-1 Antigen) Ber-H2(M) Mem and Cyt Hodgkin’s Lymphoma IVD 70

CD30 (Ki-1 Antigen) HRS-4(M)Mem(mostly perinuclear dot positivity) and cyt

Hodgkin’s Lymphoma IVD 70

CD31 (Endothelial Cell) JC/70A(M) Mem and Cyt Colon Carcinoma IVD 70

CD31 (PECAM-1) 9G11(M) Mem and Cyt Lymph node IVD 70

CD34 (Endothelial Cell) QBend/10(M) Mem Adenocarcinoma lung IVD 71

CD35 RLB25(M) Mem Tonsil IVD 71

CD35 SP191(R) Mem Tonsil IVD 71

CD41/Integrin Alpha IIb EP178(R) Mem/Cyt Spleen IVD 71

CD43 & CD45RA Cocktail MT1 &MB1(M) Mem Tonsil IVD 72

CD43 (T Cell,Leukosialin) DFT-1(M) Mem Tonsil IVD 72

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Page

CD44 (Phagocytic Glycoprotein-1,HCAM) DF1485(M) Mem Tonsil IVD 72

CD45 (Leukocyte common Antigen,LCA) PD7/26/16&2B11(M) Mem Tonsil IVD 72

CD45 (Leukocyte common Antigen,LCA) LJ27.9(M) Mem Tonsil IVD 73

CD45 Cocktail (Leukocyte Antigen, LCA) MEM55+L J 27.9(M) Mem Tonsil IVD 73

CD45RA (B Cell) MB1(M) Mem Tonsil IVD 73

CD45RB MEM55(M) Mem Tonsil IVD 73

CD45RC (T Cell) MT2(M) Mem Tonsil IVD 74

CD45RO (T Cell) UCHL-1(M) Mem Tonsil IVD 74



CD56 (Natural Killer Cell,NCAM) NKH-1(M) Mem(Frozen) Tonsil IVD 75

CD57 (Natural Killer Cell) NK-1(M) Mem and Cyt Tonsil IVD 75

CD61 EP2417Y(R) Mem Kidney IVD 75

CD63 EP211(R) Cyt/Mem Melanoma/Prostate Ca. IVD 75

CD66 BY114(M) Mem Tonsil IVD 76

CD68 KP1(M) Cyt Stomach/Lymph node IVD 76

CD68 CD68/G2(M) Cyt Histiocytoma IVD 76

CD71 (Transferrin Receptor) T9(M) Mem(Frozen) Tonsil IVD 76

CD71 (Transferrin Receptor) H68.4(M) Mem and Cyt Bone Marrow/Fetal Liver IVD 77

CD74 (B Cell) LN2(M) Mem and Cyt Tonsil IVD 77

CD79a 11E 3(M) Mem Lymph Node/Tonsil IVD 77

CD79a EP82(R) Mem/Cyt Tonsil/B-cell lymphoma IVD 77

CD90 EP56(R) Mem/Cyt Thymus IVD 78

CD95 EP208(R) Mem/Cyt Tonsil IVD 78

CD99 EPR3097Y(R) Mem Ewings Sarcoma IVD 78

CD99 (MIC2 Antigen) HO36.1.1(M) Mem Thymus/Tonsil IVD 78

CD103 EP206(R) Mem Colon IVD 79

CD105 4G11(M) Mem Uterus IVD 79

CD117 T595(M) Mem and Cyt Gastrointestinal stromal tumor/Stomach IVD 79

CD117 YR145(R) Mem and Cyt Gastrointestinal stromal tumor/Stomach IVD 79

CD146 EP54(R) Cyt/Mem Placenta IVD 80

CD205 EP176(R) Mem/Cyt Tonsil IVD 80

CD227 (MUCIN 1) VU-4H5(M) Cyt Colon/Mucinous Adenocarcinoma IVD 80

CDw75 (B Cell) LN1(M) Mem and Cyt Tonsil IVD 80

CDX-2 CDX2-88(M) Nuc Colon IVD 81

c-erbB-2 (HER-2/neu) CB11(M) Mem Breast Carcinoma IVD 81

c-erbB-2 (HER-2/neu) EP3(R) Mem Breast Carcinoma RUO 81

c-erbB-3 (HER-3) RTJ1/A2(M) Mem Breast Carcinoma IVD 81

Chromogranin A LK2H10(M) Cyt Pancreas IVD 82

Chromogranin A PHE-5(M) Cyt Pancreas IVD 82

Claudin 1 Polyclonal(R) Mem/Cell junction Breast Carcinoma or Spleen IVD 82

Claudin-5 EP224(R) Cell junction/Mem Vascular/Lung Squamous Ca. IVD 82

c-myc Protein 9E 10(M) Nuc Adenocarcinoma IVD 83

46

Primary Antibodies

www.biogenex.com



Page

Collagen III HWD1.1(M) Extracellular matrix Skin IVD 83

Collagen IV COL-94(M) Basal laminae/Cyt Skin IVD 83

Cyclin D1 Polyclonal(R) Nuc Thyroid Carcinoma/ Breast Carcinoma) IVD 83

Cyclin D1 EPR2241(IHC)-32(R) Nuc Thyroid Carcinoma IVD 84

Cyclin E2 EP454Y(R) Nuc Glioblastoma IVD 84

Cytokeratin 4 EP4(R) Cyt Esophagus IVD 84

Cytokeratin 4 6B10(M) Cyt Tonsil RUO 84

Cytokeratin 5 EPR1600Y(R) Cyt Squamous Cell Carcinoma IVD 85

Cytokeratin 5 & 6 EPR1600Y & EPR1602Y(R) Cyt Squamous Cell

Carcinoma IVD 85

Cytokeratin 5 & Cytokeratin 14 EP24/EP61(R) Cyt Prostate IVD 85


Cytokeratin 7 OV-TL12/30 Cyt Adenocarcinoma IVD 86

Cytokeratin 7 EPR1619Y(R) Cyt Breast Cancer IVD 86

Cytokeratin 7 & 8 OV-TL12/30 & C51(M) Cyt Breast Carcinoma IVD 86

Cytokeratin 8 C-51(M) Cyt Breast Carcinoma IVD 86

Cytokeratin 8 & 18 5D3(M) Cyt Colon Carcinoma IVD 87

Cytokeratin 10 DEK-10(M) Cyt Skin IVD 87

Cytokeratin 13 AE8(M) Cyt Tongue/Esophagus/ Tonsil IVD 87

Cytokeratin 14 LL002(M) Cyt Squamous Cell Carcinoma IVD 87


Cytokeratin 16 EP1615Y(R) Cyt Squamous Cell Carcinoma IVD 88

Cytokeratin 17 E27(M) CytBasal Cell carcinoma and squamous cell carcinoma tissue

IVD 88

Cytokeratin 18 DC-10(M) Cyt Breast Carcinoma IVD 88

Cytokeratin 19 RCK108(M) Cyt Colon Carcinoma IVD 89

Cytokeratin 20 IT-Ks20.8(M) Cyt Colon Carcinoma IVD 89

Cytokeratin 20 EPR1622Y(R) Cyt Colon Carcinoma IVD 89

Cytokeratin Cocktail AE1 and AE3(M) Cyt Skin IVD 89

Cytokeratin Cocktail, Broad Spectrum 34ßE12/C51/AE1(M) Cyt Skin IVD 90

Cytokeratin Cocktail, Broad SpectrumLL002+DEK-10+RCK108+OV-TL12/30+C11(M)

Cyt Breast Carcinoma IVD 90

Cytokeratin, High MW 34ßE12(M) Cyt Prostate IVD 90

Cytokeratin, High MW (Basic) AE3(M) Cyt Squamous Cell Carcinoma IVD 90

Cytokeratin, Low MW AE1(M) Cyt Adenocarcinoma IVD 91

Cytokeratin, Pan Lu-5(M) Cyt Colon Carcinoma IVD 91

Cytokeratin, Pan C11(M) Cyt Breast Carcinoma IVD 91

Cytomegalovirus (CMV) BM204(M) Nuc Lung RUO 91

Desmin D33(M) Cyt Leiomyoma IVD 92

DOG1 1.1(M) Cyt/Mem GIST Tissues IVD 92

Dystrophin Dys1(Dy4/6D3)(M) Mem Muscle IVD 92

47

Primary Antibodies

[email protected]



Page

Dystrophin Dys2(Dy8/6C5)(M) Mem Muscle IVD 92

E-Cadherin 36(M) Mem Breast Carcinoma/Colon Carcinoma IVD 93

E-Cadherin EP6(R) Mem Colon IVD 93

EGFR Polyclonal(R) Mem Squamous Carcinoma IVD 93

EGFR(PAN) EP38Y(R) Mem Squamous Carcinoma IVD 93

Ep-CAM E144(R) Mem Adenoma IVD 94

Epithelial Membrane Antigen (EMA) E29(M) Mem & Cyt Lung IVD 94

Epithelial Membrane Antigen (EMA) Mc5(M) Mem & Cyt Sebaceous Carcinoma/ Breast cancer IVD 94

Epithelial-Specific Antigen MOC-31(M) Mem Colon Carcinoma IVD 94

Epstein-Barr Virus Early Antigen 1108-1(M) Nuc/Cyt EBV Infected culture cells IVD 95

erbB-2 (HER-2/neu) EP1045Y(R) Mem Breast Carcinoma IVD 95

Estradiol Polyclonal(R) Nuc Breast Carcinoma IVD 95

Estrogen Receptor 1D5(M) Nuc Breast Carcinoma IVD 95

Estrogen Receptor EP1(R) Nuc Breast Carcinoma IVD 96

Estrogen Receptor (ER Alpha) EPR703(2)(R) Nuc Breast Carcinoma IVD 96

Estrogen Receptor (InSite® ER) ER88(M) Nuc Breast Carcinoma IVD 96

Estrogen Receptor-Beta (ER-β) Polyclonal(R) Nuc Breast Carcinoma IVD 96

Factor VIII-Related antigen F8 2.2.9(M) Cyt Leiomyoma IVD 97

Factor XIII Subunit A E980.1(M) Cyt Placenta IVD 97

Factor XIII A EP3372(R) Cyt Bone Marrow IVD 97

Fascin FCN01(M) Cyt Lymph Node IVD 97

Fibronectin F14(R) Cyt and extracellular matrix Placental tissue IVD 98

Follicle Stimulating Hormone (FSH) Polyclonal(R) Cyt Pituitary IVD 98

Gastrin Polyclonal(R) Cyt Stomach IVD 98

GCDFP-15 EP1582Y(R) Cyt Breast Carcinoma IVD 98

Glial Fibrillary Acidic Protein (GFAP) GA-5(M) Cyt Cerebellum IVD 99

Glial Fibrillary Acidic Protein (GFAP) Polyclonal(R) Cyt Cerebellum IVD 99

Glomerular Epithelial Protein 1 (GLEPP-1) 5C11(M) Mem Kidney IVD 99

Glucagon Polyclonal(R) Cyt Pancreas IVD 99

Glut-1 SPM498(M) Mem/Cyt Esophagus Carcinoma/ Squamous Carcinoma IVD 100

Glutathione S-Transferase Pi (GST Pi) Polyclonal(R) Nuc & Cyt Breast IVD 100

Glypican-3 (GPC3) GPC3-88(M) Cyt/Mem Hepatocellular carcinoma RUO 100

Granulocyte BM-2(M) Cyt Hodgkin IVD 100

Growth Hormone (Hgh) Polyclonal(R) Cyt Pituitary IVD 101

Heat Shock Protein (HSP-70) BRM-22(M) Cyt Breast Carcinoma IVD 101

Heat Shock Protein 27 (HSP 27) G3.1(M) Cyt Breast Carcinoma IVD 101

Helicobacter pylori Polyclonal(R) Bacterium Stomach IVD 101

Hemoglobin A Polyclonal(R) Cyt Placenta IVD 102

Hepatitis B Virus Core Antigen (HBcAg) Polyclonal(R) Nuc Liver RUO 102

Herpes Simplex Virus Type I (HSV I) Polyclonal(R) Nuc/Cyt Lung IVD 102

Herpes Simplex Virus Type II (HSV II) Polyclonal(R) Nuc/Cyt Lung IVD 102

HLA-DR LN3(M) Mem Tonsil RUO 103

48

Primary Antibodies

www.biogenex.com



Page

HSA HSA/E8(M) Cyt Liver Tissues IVD 103

Human Chorionic Gonadotropin (hCG) Beta M94138(M) Cyt Placenta IVD 103

IgA Polyclonal(R) Mem & Cyt Tonsil IVD 103

IgD Polyclonal(R) Mem & Cyt Tonsil IVD 104

IgG IgG88(R) Mem & Cyt Tonsil IVD 104

IgG Polyclonal(R) Mem & Cyt Tonsil IVD 104

IgM IgM88(M) Mem & Cyt Tonsil IVD 104

IgM Polyclonal(R) Mem & Cyt Tonsil IVD 105

Inhibin-Alpha R1(M) Cyt Ovary IVD 105

Insulin HB125(M) Cyt Pancreas IVD 105

Insulin EP125(R) Cyt Pancreas/Insulinoma IVD 105

J Chain JC88(M) Cyt Tonsil IVD 106

Kappa Light Chain L1C1(M) Cyt Tonsil IVD 106

Kappa Light Chain K88(M) Cyt Tonsil IVD 106

Ki-67 K-2(M) Nuc Tonsil IVD 106

Ki-67 EPR3611(R) Nuc Breast Carcinoma/Tonsil IVD 107

Ki-67 EP5(R) Nuc Tonsil IVD 107

Ki-67 Antigen, Proliferating Cell MIB-1(M) Nuc Lymphoma IVD 107

Ki-67 Antigen, Proliferating Cell Ki88(M) Nuc Tonsil IVD 107

Lambda Light Chain Polyclonal(R) Cyt Tonsil IVD 108

Lambda Light Chain EP172(R) Mem/Cyt Tonsil/B-cell lymphoma IVD 108

Laminin Polyclonal(R) Basement Mem Bronchus IVD 108

Lysozyme Polyclonal(R) Cyt Tonsil,Lymph Node IVD 108

Macrophage LN5(M) Cyt Liver Tissues IVD 109

Mast Cell Tryptase AA1(M) Cyt Skin IVD 109

Melan-A (MART-1) A103(M) Cyt Melanoma IVD 109

Melanoma HMB45(M) Cyt Melanoma IVD 109

Melanoma gp100 gp100/D5(M) Cyt Melanoma tissue IVD 110

Melanoma-Associated Antigen NKI/C3(M) Cyt/Mem Melanoma IVD 110

Mesothelin 5B2(M) Mem Ovary Cyst Adenocarcinoma IVD 110

MiTF MiTF/A13(M) Nuc Melanoma Tissue IVD 110

Mitochondrial Antigen 113-1(M) Cyt Breast Carcinoma IVD 111

MLH1 ES05(M) Nuc Colon Tissue IVD 111

MMP-9 EP1255Y(R) Cyt Bone Marrow IVD 111

MSH2 SP46(R) Nuc Colon IVD 111

MSH6 2D4B5(M) Nuc Colon Carcinoma IVD 112

MUC4 1G8(M) Cyt Colon Carcinoma IVD 112

MUC5AC 45M1(M) Cyt Stomach Mucosa/ Gastrointestinal tissue IVD 112

Mucin 1 (MUC1) EPR1023(R) Cyt Breast Carcinoma IVD 112

Mucin 2 (MUC2) CCP58(M) Cyt Colon Carcinoma IVD 113

Multi-Drug Resistance Marker (P-Glycoprotein) MDR88(M) Mem & Cyt Adrenal Gland IVD 113

Muscle Actins Actin 88 Cocktail(M) Cyt Muscle IVD 113

Myelin Basic Protein MBP88(M) Cyt Cerebellum IVD 113

Myeloid specific Antigen BM-3(M) Cyt Bone Marrow IVD 114

Myeloid specific Antigen BM-1(M) Cyt & Nuc Lymph node,Tonsil,Bone Marrow IVD 114

49

Primary Antibodies

[email protected]



Page

Myeloperoxidase (MPO) Polyclonal(R) Cyt Spleen IVD 114

Myf4 LO26(M) Nuc Rhabdomyosarcoma IVD 114

Myoglobin MG-1(M) Cyt Muscle IVD 115

Myoglobin Polyclonal(R) Cyt Muscle IVD 115

Myosin Heavy Chains,Smooth Muscle SMMS.1(M) Cyt Breast Carcinoma IVD 115

Myosin,Skeletal Muscle MY-32(M) Cyt Muscle IVD 115

Napsin A IP64(M) Cyt Lung Adenocarcinoma IVD 116

N-Cadherin EPR1792Y(R) Mem Colon Carcinoma Tissue IVD 116

Neurofilament NE-14(M) Cyt Nerve IVD 116

Neuron Specific Enolase (NSE) MIG-N3(M) Cyt Pancreas/Nerve IVD 116

NGF Receptor EP1039Y(R) Mem Neuroblastoma IVD 117

NGF Receptor EP31(R) Cyt Pancreas/Melanoma IVD 117

Nuclear Ribonucleoprotein 58-15(M) Nuc Spleen IVD 117

Oct 2 EPR542(R) Nuc Tonsil tissues IVD 117

Oct 4 EP143(R) Nuc Seminoma in Testis IVD 118

Osteonectin OST1(M) Cyt Osteosarcoma IVD 118

p105 Proliferation-Associated Nuclear Antigen 2B3(M) Nuc Tonsil IVD 118

p16 (INK4a) G175-405(M) Nuc & Cyt Sqamous Cell Carcinoma RUO 118

p21/WAF1 4D10(M) Nuc Melanoma IVD 119

p27 (Kip1) DCS72(M) Nuc Breast Carcinoma IVD 119

p27 (Kip1) Y236(R) Nuc Breast Carcinoma IVD 119

p34cdc2 (Cyclin Dependent Kinase) POH-1(M) Nuc & Cyt Tonsil IVD 119

P504S (AMACR) 13H4(R) Cyt Prostate carcinoma IVD 120

P504S (AMACR) RBT-AMACR(R) Cyt Prostate carcinoma IVD 120

p53 EP9(R) Nuc Breast Ca. IVD 120

p53 Protein BP53-12-1(M) Nuc Breast Carcinoma IVD 120

p53 Protein DO7(M) Nuc Breast Carcinoma IVD 121

p53 Protein 1801(M) Nuc Breast Carcinoma IVD 121

p63 Protein 484(M) Nuc Prostate IVD 121

PAP A40010(M) CytBenign Prostate hyperplasia(BPH) and carcinoma prostate

IVD 121

Papillomavirus Type 16 (HPV-16) CamVir-1(M) Nuc Cervix IVD 121

PAX-5 ZP007(M) Nuc Lymph Node IVD 122

Paxillin (N-Term) Y113(R) Cyt Breast Carcinoma IVD 122

PDCD4 EPR3432(R) Cyt/Nuc Breast Carcinoma IVD 122

PDGF Receptor-ß Y92(R) Mem GIST IVD 122

PGP9.5 3D9(M) Cyt Brain/Small Intestine IVD 123

Placental Alkaline Phosphatase (PLAP) PL8-F6(M) Cyt Placenta IVD 123

Placental Lactogen (hPL) Polyclonal(R) Cyt Placenta IVD 123

Platelet-Derived Growth Factor (PDGF) PDGF88(M) Cyt Squamous IVD 123

Platelet-Derived Growth Factor (PDGF) Polyclonal(R) Cyt Squamous IVD 124

PMS2 EPR3947(R) Nuc Colon Carcinoma IVD 124

Progesterone Receptor EP2(R) Nuc Breast IVD 124

Progesterone Receptor 1A6(M) Nuc Breast IVD 124

Progesterone Receptor (InSite®PR) PR88(M) Nuc Breast Carcinoma IVD 125

Prolactin ME.121(M) Cyt/Mem Pituitary IVD 125

50

Primary Antibodies

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Page

Proliferating Cell Nuclear Antigen (PCNA) PC10(M) Nuc Colon Carcinoma IVD 125

Prostate Specific Acid Phosphatase (PSAP) B01-94-21M-NA(M) Cyt Prostate Hyperplasia RUO 125

Prostate Specific Antigen (PSA) ErPr-8(M) Cyt Prostate/Prostate Carcinoma RUO 126

pS2 Estrogen Inducible Protein PS2.1(M) Cyt Breast Carcinoma IVD 126

PSMA EP192(R) Cyt/Mem Prostate Ca. IVD 126

RCC (Renal Cell Carcinoma) RCC-26(M) Cyt/Mem Kidney,Renal Cell Carcinoma IVD 126

S100 beta EP32(R) Cyt Prostate Ca. IVD 127

S100 Protein Polyclonal(R) Nuc and Cyt Melanoma IVD 127

S-100 Protein 15E2E2(M) Nuc & Cyt Melanoma IVD 127

S100P EP186(R) Cyt/Nuc Placenta/Pancreatic Adeno Ca. IVD 127

Sarcomeric Actin ZMSA-5(M) Cyt Muscle IVD 128

Secretin Polyclonal(R) Cyt Stomach IVD 128

Stat 5-Alpha (C-term) E289(R) Cyt & Nuc Breast Carcinoma IVD 128

Substance P Polyclonal(R) Cyt Hypothalamus IVD 128

Survivin EP2880Y(R) Nuc/Cyt TCC Bladder tissue/ Bladder carcinoma IVD 129

Synaptophysin Snp88(M) Cyt Pancreas IVD 129

Tau Tau-2(M) Cyt Cerebellum IVD 129

Tau Tau-5(M) Cyt Brain IVD 129

Terminal Deoxynucleotidyl Transferase (TdT) TdT88(M) Nuc Thymus IVD 130

Terminal Deoxynucleotidyl Transferase (TdT) Polyclonal(R) Nuc Thymus IVD 130

TFPU.1 EPR3159Y(R) Nuc Lymphoma IVD 130

Thyroglobulin 2H11(M) Cyt Follicular adenoma IVD 130

Thyroid Stimulating Hormone (TSH) 5404(M) Cyt Follicular Adenoma IVD 131

Thyroid Stimulating Hormone (TSH) Polyclonal(R) Cyt Follicular Adenoma IVD 131

Thyroid Transcription Factor (TTF-1) 8G7G3/1(M) Nuc Lung IVD 131

Thyroxine D5(M) Cyt Thyroid IVD 131

TIA-1 2G9A10F5(M) Cyt T-cell lymphoma tissue IVD 132

Topo 2 Alpha EP1102Y(R) Nuc/Cyt Thyroid Carcinoma/ Breast Carcinoma) IVD 132

Toxoplasma gondii Polyclonal(R) T.gondii Brain RUO 132

Transferrin HT1/13.6.3(M) Cyt Liver/Muscle IVD 132

Transforming Growth Factor (TGF),Alpha TGF88(M) Cyt Breast Carcinoma IVD 133

Tumor-Associated Glycoprotein (TAG-72) B72.3(M) Cyt Breast Carcinoma IVD 133

Tumor-Associated Glycoprotein (TAG-90 BCA) B6.2(M) Cyt Breast Carcinoma IVD 133

Tyrosinase Ty/G5(M) Cyt Melanoma IVD 133

VEGF Polyclonal(R) Cyt Angiosarcoma IVD 134

Villin ID2C3(M) Cyt and brush border Small Intestine IVD 134

Vimentin V9(M) Cyt Skin/Leiomyoma IVD 134

Vimentin,Non-Hematopoietic LN6(M) Cyt Leiomyoma IVD 134

VIP Polyclonal(R) Cyt Small Intestine IVD 135

Wilm’s Tumor CAN-R9(IHC)-56-2(R) Nuc Wilm’s Tumor IVD 135

ZAP-70 ZAP70-C3(M) Cyt/Mem Tonsil and Reactive lymph node IVD 135

ZAP-70 YE291(R) Mem/Cyt Tonsil IVD 135

51

Primary Antibodies

P - Tissue Type FFPE F - Tissue Type Frozen [email protected]

Aberrant Endothelial Cell

In an in vivo model of allergic contact dermatitis, 4A11 antigen was differentially upregulated from other endothelial markers such as E-selectin, vascular cell adhesion molecule-1, and intercellular adhesion molecule-1. Monoclonal antibody 4A11 reacts with the H-5-2 and Lewis Y-6 blood group glycolipids. This antibody reacts with vascular endothelial cells in lymphoid tissues and endothelial cells in diseased tissue such as rheumatoid and osteoarthritic synovium, psoriatic skin, adrenal tumors and cutaneous Kaposi’s sarcomas. It does not react with several myeloid or lymphoid cell lines, peripheral blood cells and platelets. It does not detect endothelium of medium-sized vessels and that of normal tissues such as liver and spleen.

Ready-to-use (Manual):

Concentrated:

AM382-5M

MU382-UC

Tonsil stained with Anti-Aberrant Endothelial cell using AEC chromogen

Clone: 4A11

Isotype: IgM

Source: Mouse

Immunogen: Human rheumatoid cells

Specificity: Vascular endothelial cell Antigen

Localization: Cytoplasm

Pre-treatment: EZ-AR1

Xmatrx: HX031-YCDE

P

AX382-YCDReady-to-use (Automated): Xmatrx®

ACTH

Adrenocorticotropic hormone (ACTH or Corticotropin) is a polypeptide tropic hormone produced and secreted by the anterior pituitary gland. It is an important component of the hypothalamic-pituitary-adrenal axis and is often produced in response to biological stress (along with corticotrophin-releasing hormone from the hypothalamus). Its principal effects are increased production of androgens and as its name suggests, cortisol from the adrenal cortex. It labels corticotrophs in the adenohypophysis and is useful in the classification of pituitary adenomas.

Ready-to-use (Manual): AM487-5M

AM487-10M

MU487-UCConcentrated:

FG-487NRecommended Positive Control:

Pituitary tissue stained with Anti-ACTH using DAB chromogen

Clone: AH26

Isotype: IgG

Source: Mouse

Immunogen: A synthetic peptide corresponding to amino acids1-24 from the N-terminal of human ACTH

Specificity: ACTH


Pre-treatment: EZ-AR™ 2/ EZ-AR1

Manual/i6000: HK522-XAK

Xmatrx: HX031-YCDE

P

Ready-to-use (Automated): i6000TM Xmatrx® AX487-YCD

Actin, Muscle-Specific

Actin, a major component of the cytoskeleton, is a globular protein about 5 nm in diameter and is composed of one polypeptide chain with a mass of approximately 47kD. This antibody recognizes alpha actin of skeletal, cardiac and smooth muscle cells and gamma actin from smooth muscle cells. It is non-reactive with other mesenchymal cells and all epithelial cells except for myoepithelium. It can be used to stain leiomyomas, leiomyosarcomas, rhabdomyomas and rhabdomyosarcomas. This antibody labels cytoplasm in skeletal, cardiac and smooth muscle cells.


Concentrated:

Recommended Positive Control:

Recommended Barrier Control:

AM090-5M

AM090-10M AX090-YCD

MU090-UC

FG-090M

FB-090M

Clone: HHF35

Isotype: IgG1

Source: Mouse

Immunogen: Homogenized human myocardium

Specificity: Muscle-specific Actin


Pre-treatment: None

Heart muscle stained with Anti-Muscle Specific Actin using DAB chromogen

P

Ready-to-use (Automated): i6000TM Xmatrx®

Actin, Smooth Muscle

Actin is one of the two major cytoskeletal proteins. The antibody can be used to identify smooth muscle tumors. It stains leiomyomas and leiomyosarcomas but does not stain carcinomas, melanomas, lymphomas or non-smooth muscle sarcomas. It stains the muscularis and muscularis mucosa of the gastrointestinal tract, the uterine myometrium, medial layer of blood vessels, the mesenchymal components of the prostate, and myoepithelial cells of salivary glands and other organs. The antibody does not stain striated muscle such as skeletal and cardiac muscle, endothelium, connective tissue, epithelium or nerve. This antibody stains positive in cytoplasm of smooth muscle cells.


Concentrated:



AM128-5M

MU128-UC

FG-128M

FB-128M

Clone: 1A4

Isotype: IgG2a

Source: Mouse

Immunogen: Synthetic NH2 terminal decapeptide of alpha smooth muscle actin coupled to keyhole limpet hemocyanin (KLH)

Specificity: Alpha Smooth Muscle Actin


Pre-treatment: None

Stomach tissue stained with Anti-Smooth Muscle Actin using DAB chromogen

P

AM128-10M AX128-YCD


Primary Antibodies

52P - Tissue Type FFPE F - Tissue Type Frozen www.biogenex.com

Adenovirus


Concentrated:

AM059-5ME

MU059-UCE

Adenoviruses are simple DNA-containing viruses that multiply in the cell nucleus. They induce latent infections in tonsils, adenoids and other lymphoid tissue of man, causing either inapparent or limited illnesses that are followed by complete recovery and persistent type-specific immunity. Most individuals are infected with one or more adenovirus before the age of 15. Approximately 50-80% of tonsils and adenoids removed surgically show adenovirus infection. This antibody is designed for the specific immunohistochemical localization of adenovirus. Monoclonal antibodies directed against viruses allow for the rapid identification of viral infections in tissues. The advantages of identifying viruses immunohistologically are twofold. First, this method decreases the time required for microbial identification by viral culture, and second, this method may demonstrate virus in tissues in which characteristic cytopathic effects have not yet developed and are therefore not detectable by morphological studies alone.

Clone: A62020069P

Isotype: IgG1 Kappa

Source: Mouse

Immunogen: Adeno 3 strain

Specificity: Adenovirus antigen/immunogen in frozen tissue sections or infected cell culture.

Localization: Nucleus & Cytoplasm

Pre-treatment: EZ-AR 1/EZ-AR 2

Xmatrx: HX031-YCDE/HX032-YCDE

Adenovirus cell culture stained using AEC chromogen

F

FG-059MRecommended Positive Control:

AX059-YCDEReady-to-use (Automated): Xmatrx®

Alpha-1-Antichymotrypsin

Alpha-1-Antichymotrypsin (ACT) is a serine protease inhibitor. It forms a complex with serine protease, a prostate-specific antigen in human serum. ACT can be found in most cells of myeloid lineage and is, therefore, useful in the identification of neoplastic myeloid cells within extramedullary tissues such as acute myeloid leukemia. This enzyme is also localized in the spindle cells and round cells of true histiocytic lymphomas as well as in most thyroid papillary carcinomas. ACT is expressed in various normal and neoplastic cells. The mouse monoclonal antibody stains ACT protein in the cytoplasm of many different cells.


Concentrated:

AM388-5M

MU388-UC

Clone: α1A88

Isotype: IgG1, Kappa

Source: Mouse

Immunogen: Biochemically purified alpha-1-antichymotrypsin protein was used to sensitize Balb/c (H-2d) mice

Specificity: Alpha-1-Antichymotrypsin protein


Pre-treatment: EZ-AR 1

Xmatrx HX031-YCDE

Liver stained with Anti-ACT using AEC chromogen

P

Ready-to-use (Automated): Xmatrx® AX388-YCD

Alpha-1-Antitrypsin

Alpha-1-Antitrypsin (AAT) is a 54 kD glycoprotein. Most of the anti-proteolytic enzyme activity of serum resides in this fraction. It is also found in lymph, mucus, saliva, synovial fluid, gastrointestinal tract secretions, semen, amniotic fluid and colostrum. It is a useful marker for benign and malignant hepatic neoplasms, endodermal sinus tumors, and for histiocytic differentiation in benign and malignant fibrous histiocytomas. This antibody has been absorbed with fractionated human plasma to remove contaminating antibodies. When tested by crossed immunoelectrophoresis against human plasma, a single precipitin line was observed.

Liver tissue stained with Anti-alpha-1-Antitrypsin using AEC chromogen

Clone: Polyclonal

Isotype: N/A

Source: Rabbit

Immunogen: Human plasma

Specificity: Alpha-1-Antitrypsin




P

Ready-to-use (Manual): AR015-5R

Concentrated:


PU015-UP

FG-015P

AW015-YCDReady-to-use (Automated): Xmatrx®

Alpha-Actinin

Actinins are actin-binding proteins of 100 kD. Alpha-Actinin is an F-actin cross-linking protein thought to anchor actin to a variety of intracellular structures. Alpha-Actinin is found in stress fibers and adhesion plaques in non-muscle cells and in Z-discs and their homologues in muscle cells.


Concentrated:


AM097-5M

MU097-UC

FG-097M

Muscle stained with Anti-alpha actinin using AEC chromogen

Clone: JLN20

Isotype: IgM

Source: Mouse

Immunogen: Alpha-actinin isolated from chicken gizzard

Specificity: Alpha-Actinin




P


53

Primary Antibodies


Alpha-Fetoprotein (AFP)

Alpha-Fetoprotein (AFP) is a 64 kD tumor-associated embryonal antigen produced by fetal liver, hepatoma, yolk sac, and several germ cell tumors of testicular and ovarian origin. Of the germ cell tumors, only embryonal carcinoma and endodermal sinus tumors stain positive for AFP and not teratomas. The positive results are useful in distinguishing embryonal carcinoma from seminoma. AFP is present in the mononuclear embryonal carcinoma cell and in the intracellular or extracellular hyaline droplets. This antibody stains positive for alpha fetoprotein in the cytoplasm of positive cells.

Hepatocellular carcinoma stained with Anti-AFP using AEC chromogen

Clone: C3

Isotype: IgG 2a

Source: Mouse

Immunogen: Affinity-purified human Alpha-Fetoprotein

Specificity: Alpha-Fetoprotein


Pre-treatment: None

Recommended Positive Control: FG-008M

Recommended Barrier Control: FB-008M

Concentrated: MU008A-UC


P

AM008-10MReady-to-use (Automated): i6000TM Xmatrx® AX008-YCD

Alpha-Tubulin

Microtubules, along with microfilaments and intermediate filaments, form the major part of the extensive cytoplasmic network known as the cytoskeleton. The thickest of these filaments are the 20-25 nm microtubules composed of tubulin and several additional microtubule-associated proteins (MAP). Tubulin is a heterodimer composed of α-tubulin and β-tubulin. Each subunit is a 55 kD acidic protein. Tubulin assembles into the microtubule system during interphase, then reassembles into the mitotic spindle during cell division. This antibody reacts specifically with the alpha subunit of tubulin in cultured chicken fibroblasts, human, bovine, murine, and amphibian cells, and also in yeast and fungi.

Concentrated:


MU121-UC

FG-121M

Clone: DM-1A

Isotype: IgG1

Source: Mouse

Immunogen: Alpha-Tubulin isolated from chick brain microtubules

Specificity: Alpha-Tubulin


Pre-treatment: None

Lung tissue stained with Anti-Alpha-Tubulin using AEC chromogen

P

Androgen Receptor

This monoclonal antibody is specifically designed to recognize a unique immunogenic N-terminal transactivation domain of the androgen receptor that has a low degree of homology with other steroid receptors. This antibody binds to synthetic peptide SP61 of human androgen receptor. This antibody does not cross-react with human estrogen, progesterone or glucocorticoid receptor.

Ready-to-use (Manual 3mL):


Concentrated:

AM256-2ME

AM256-10ME

MU256-UCE

Clone: F39.4.1

Isotype: IgG1 Kappa

Source: Mouse

Immunogen: Synthetic peptide sequence comprising amino acids 301-320 of human androgen receptor (SP61).

Specificity: Androgen Receptor antigen

Localization: Nuclear

Pre-treatment: AR Citra/EZ AR-2

Manual/i6000: HK087-5KE

Xmatrx: HX032-YCDE

Prostate Hyperplasia showing nuclear Androgen Receptor positivity using DAB chromogen

P

Recommended Barrier Control: FB-256MFG-256MRecommended Positive Control:

AX256-YCDE

Apolipoprotein E

Apolipoprotein E (ApoE) is essential for the normal catabolism of triglyceride rich lipoprotein constituents. Defects in the ApoE gene will result in familial dysbetalipoproteinemia or type III hyperlipoproteinemia and will increase cholesterol and triglycerides levels in the blood. Maintaining normal levels of cholesterol is essential for the prevention of cardiovascular diseases, including heart attacks and strokes. There are three isoforms of the ApoE lipoprotein, known as ApoE2, E3 and E4. ApoE4 has been associated with an increased probability of developing Alzheimer disease.

Ready-to-use (Manual): AN567-5M

AN567-10M

NU567-UCConcentrated:

Hepatocellular carcinoma stained with Anti-Apolipoprotein E using DAB as chromogen

Clone: EP1373Y

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues near the N-term of human apolipoprotein E

Specificity: Apolipoprotein E

Localization: Membrane and Cytoplasm

Pre-treatment: AR Citra Plus /EZ-AR 1/EZ-AR 2



Recommended Positive Control: FG-567N

P


Primary Antibodies


B Cell

MB2 reacts with a cytoplasmic antigen present in all B cells with the exception of plasma cells. It also reacts with endothelial cells and various types of epithelial cells. MB2 shows no reaction with T lymphocytes or thymocytes. A faint staining may occur when using frozen sections containing T cells. MB2 is not suitable for immunolabeling of living or unfixed cells.


Concentrated:

AM158-5M

MU158-UC


Clone: MB2

Isotype: IgG1

Source: Mouse

Immunogen: Hodgkin’s lymphoma cell line DEV

Specificity: MB2



Manual/i6000: None

Xmatrx: HX031-YCDETonsil tissue stained with Anti-B cell using Fast Red Chromogen

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AM158-10M AX158-YCD


B Lymphocyte Antigen 36 (BLA.36)

BLA.36 is a developmentally regulated 36 kD antigen expressed on the plasma membrane of B lymphocytes, Reed-Sternberg, and mononuclear Hodgkin’s cells. The anti-BLA.36 antibody recognizes all four subtypes of Hodgkin's disease. It also gives strong staining of B cell lymphomas including follicular center cell lymphomas (large and small cell types), mantle zone lymphomas, and immunoblastic lymphomas. No reactivity of anti-BLA.36 is found in normal epithelial cells, including adrenal gland, breast, colon, lung, salivary gland, skin, stomach and their malignant counterparts. Anti-BLA.36 can be used to distinguish Reed-Sternberg cells and some B-cell lymphomas from other malignant cells.


Concentrated:

AM231-5M

AM231-10M

MU231-UC



Clone: A27-42

Isotype: IgG3

Source: Mouse

Immunogen: Hodgkin's cell line HDLM-3

Specificity: BLA.36 antigen

Localization: Membrane


Manual/i6000: None

Xmatrx: HX031-YCDE/HX032-YCDEHodgkin stained with Anti-BLA.36 using DAB chromogen

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Bax Protein

Bax protein is identified as a promoter of apoptosis. The override of apoptotic control is suspected to cause or contribute to some forms of carcinogenesis. This antibody will detect the α, ß , and d isoforms of Bax protein.

Breast carcinoma stained with Anti-Bax Protein using DAB chromogen

Clone: Polyclonal

Source: Rabbit

Immunogen: A synthetic peptide encompassing a unique epitope at the amino terminus of human Bax protein coupled to Keyhole Limpet Hemocyanin (KLH)

Specificity: Bax protein




P


Concentrated: PU347-UPAW347-YCD

Ready-to-use (Automated): Xmatrx®

Bcl-2

Over-expression of Bcl-2 has been shown to promote cell survival by suppressing apoptosis. It has been documented that Bcl-2 becomes deregulated in tumor cells as a result of translocation into the immunoglobulin heavy-chain locus and is therefore activated in B cell malignancies.


AN541-10M


Follicular cell lymphoma stained with Rabbit Anti-Bcl-2 using DAB as a chromogen

Clone: E17

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues between BH3 and BH4 of human Bcl-2

Specificity: Bcl-2 protein


Pre-treatment: EZ-AR™ 1


Xmatrx: HX031-YCDE


P


55

Primary Antibodies


Bcl-2

Bcl-2 (B-cell lymphoma 2), encoded in humans by the Bcl-2 gene, is the founding member of the Bcl-2 family of regulator proteins that regulate cell death, by either inducing (pro-apoptotic) it or inhibiting it (anti-apoptotic). Bcl-2 is specifically considered as an important anti-apoptotic protein and is thus classified as an oncogene. Over expression of Bcl-2 has been shown to promote cell survival by suppressing apoptosis. It has been documented that Bcl-2 becomes deregulated in tumor cells as a result of translocation into the immunoglobulin heavy-chain locus and is therefore activated in B cell malignancies. Bcl-2 is useful in differentiation of follicular lymphoma from reactive follicular proliferation (Bcl-2 negative). In addition, Bcl-2 has been shown to be correlated with disease prognosis in breast cancer, prostate and ovarian cancer.

Tonsil stained with Anti-Bcl 2 using DAB chromogen

Clone: EP36

Isotype: IgG1

Source: Rabbit

Immunogen: BCl-2

Specificity: BCl-2


Pre-treatment: AR-10

Manual/i6000: HK058-5K

Xmatrx: None

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AN723-10MReady-to-use (Automated): i6000TM Xmatrx® AY723-YCDRecommended Positive Control: FG-723NRecommended Barrier Control: FB-723N

BcI-2 Oncoprotein

The Bcl-2 is an integral inner mitochondrial membrane protein and is frequently overexpressed in many lymphoid malignancies. Immunohistologic studies have demonstrated that staining for Bcl-2 protein can be used to distinguish neoplastic germinal centers from reactive ones.


Recommended Positive Control: FG-287MRecommended Barrier Control: FB-287M

Tonsil tissue stained with Anti-Bcl-2 using DAB chromogen

Clone: bcl-2/100

Isotype: IgG1 kappa

Source: Mouse

Immunogen: Synthetic peptide comprising residues 41-54 of Bcl-2 oncoprotein-3

Specificity: Bcl-2 protein


Pre-treatment: AR Citra/EZ-AR 1


Xmatrx: HX031-YCDE

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AM287-10M AX287-YCD

Bcl-6

Anti-BCl-6 is a transcriptional regulator gene which codes for a 706 amino acid nuclear zinc finger protein. This antibody reacts with Bcl-6 gene product in follicular lymphomas, diffuse large B-cell lymphomas. Burkitt’s lymphomas and in nodular lymphocyte predominant Hodgkin’s disease. The antibody gives a strong nuclear labeling of BCL6 protein in follicular lymphomas, diffuse large B-cell lymphomas, Burkitt’s lymphomas and nodular, lymphocyte=predominance Hodgkin’s disease. BCl-6 is not expressed in B-CLL, hairy cell leukemia, mantle and marginal-Zone derived lymphomas.

Tonsil stained with Anti-Bcl-6 using DAB chromogen

Clone: LN22

Isotype : IgG

Source: Mouse

Immunogen: Bcl-6

Specificity: Bcl-6




Xmatrx: None

P


AM708-10MReady-to-use (Automated): i6000TM Xmatrx® AY708-YCD


FB-708MRecommended Barrier Control:

Bcl-x

Bcl-x, a member of the Bcl-2 protein family, inhibits cell death, or apoptosis. Bcl-x is expressed as two isomeric forms, Bcl-xL and Bcl-xS, and is typically present in the cytosol in association with the mitochondrial membrane. Bcl-xL forms heterodimers with various proteins, including Bax, Bak and Bcl-2. It has been found that heterodimerization with Bax does not seem to be required for anti-apoptotic activity. Since Bcl-xL can form an ion channel in synthetic lipid membranes, there is a strong possibility that this property plays a role in heterodimerization-independent cell survival. The Bcl-xS isoform promotes apoptosis.

Breast carcinoma showing cytoplasmic expression of Bcl-x stained using DAB chromogen

Clone: E18

Isotype: IgG

Source: Rabbit

Immunogen: Human Bcl-x

Specificity: Bcl-x


Pre-treatment: EZ-ARTM 1


Xmatrx: HX031-YCDE


Concentrated:


AN462-5M

AN462-10M

NU462-UC

FG-462N

P


Primary Antibodies


Beta-Catenin

ß-catenin is a key regulatory protein involved in cell adhesion and signal transduction through the Wnt pathway, and plays important roles in development, cellular proliferation, and differentiation. ß-catenin is phosphorylated at four serine and threonine residues at the N-terminus. Ser 45 is phosphorylated by CK1, and is thought to be a priming site for the phosphorylation by glycogen synthase kinase-3 (GSK-3) of the remaining three residues, Thr 41, Ser 37, and Ser 33. Wnt signaling antagonizes the action of GSK-3, which in subsequent phosphorylation steps, leads to its degradation. Mutations in the catenin gene, CTNNB1, have been implicated in its accumulation and various forms of carcinomas.


AN510-10M



Colon stained with Antiß-Catenin using DAB chromogen

Clone: E247

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues near N- terminus of human β - catenin.

Specificity: ß-Catenin

Localization: Membrane/Cytoplasm/Nucleus

Pre-treatment: EZ-AR™ 1/EZ-AR 2


Xmatrx: HX031-YCDE

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MU122-UC

FG-122M

Beta-Tubulin

Microtubules, along with microfilaments and intermediate filaments, form the major part of the extensive cytoplasmic network known as the cytoskeleton. The thickest of these filaments are the 20-25 nm microtubules composed of tubulin and several additional microtubule-associated proteins (MAP). Tubulin is a heterodimer composed of α-tubulin and ß-tubulin. Each subunit is a 55 kD acidic protein. Tubulin assembles into the microtubule system during interphase, then reassembles into the mitotic spindle during cell division. Immunoblot analysis shows that this antibody binds to the beta subunit of tubulin from cultured fibroblasts and chick brain tubulin. This antibody labels the cytoplasmic network of microtubules and mitotic spindles of cultured cells.

Concentrated:


Clone: DM-1B

Isotype: IgG1 kappa

Source: Mouse

Immunogen: Beta-tubulin isolated from chick brain microtubules

Specificity: Beta-Tubulin


Pre-treatment: None

Lung tissue stained with Anti-Beta Tubulin using AEC chromogen

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Beta-Tubulin II

Microtubules play critical roles in a variety of cellular processes, such as mitosis, intracellular transport, ciliary and flagellar motility, and maintenance of cell shape. The structural subunit of microtubules, the 100 kD protein tubulin, is a heterodimer of two 50 kD subunits designated alpha and beta. Both alpha and beta occur as numerous isotypes which differ from each other in their amino acid sequences and tissue distribution. The majority of the differences among the isotypes cluster in the C-terminal, a region where the microtubule-associated proteins (MAPs) bind to tubulin. This antibody stains ß-tubulin in cytoplasm of neuroepithelial cells and other positive cells.

Concentrated: MU176-UC


Colon stained with Anti-Beta Tubulin II using AEC chromogen

Clone: JDR3B8

Isotype: IgG2b

Source: Mouse

Immunogen: Cys-Glu-Gly-Glu-Glu-Asp-Glu-Ala-OH synthetic peptide conjugated with BSA.

Specificity: ß-Tubulin II


Pre-treatment: None

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Beta-Tubulin III

Microtubules play critical roles in a variety of cellular processes, such as mitosis, intracellular transport, ciliary and flagellar motility, and maintenance of cell shape. The structural subunit of microtubules, the 100 kD protein tubulin, is a heterodimer of two 50 kD subunits designated alpha and beta. Both alpha and beta occur as numerous isotypes which differ from each other in their amino acid sequences and tissue distribution. The majority of the differences among the isotypes cluster in the C-terminal, a region where the microtubule-associated proteins (MAPs) bind to tubulin. This antibody stains beta tubulin in cytoplasm of positive cells.



Clone: SDL3D10

Isotype: IgG2b

Source: Mouse

Immunogen: Cys-Glu-Ser-Glu-Ser-Glu-Gln-Gly-Pro-Lys-OH synthetic peptide conjugated with BSA.

Specificity: ß-Tubulin III


Pre-treatment: NoneHeart muscle stained with Anti-Beta Tubulin III using DAB chromogen

P

57

Primary Antibodies


Beta-Tubulin IV

Microtubules play critical roles in a variety of cellular processes, such as mitosis, intracellular transport, ciliary and flagellar motility, and maintenance of cell shape. In the structural subunit of microtubules, the 100 kD protein tubulin, is a heterodimer of two 50 kD subunits designated alpha and beta. Both alpha and beta occur as numerous isotypes which differ from each other in their amino acid sequences and tissue distribution. The majority of the differences among the isotypes cluster in the C-terminal, a region where the microtubule-associated proteins (MAPs) bind to tubulin. This antibody stains ß-tubulin in cytoplasm of positive cells.



Clone: ONS1A6

Isotype: IgG1

Source: Mouse

Immunogen: Cys-Glu-Ala-Glu-Glu-Glu-Val-Ala-OH synthetic peptide conjugated with BSA 1

Specificity: ß-Tubulin IV


Pre-treatment: NoneLung stained with Anti-Beta Tubulin IV using AEC chromogen

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bFGF (Basic Fibroblast Growth Factor)

bFGF is a pro-angiogenic cytokine which is present in diverse tissues. It is known to function as an autocrine mediator of mitogenesis of endothelial cells in vivo, resulting in angiogenesis. It also increases fibroblast production of plasminogen activator and collagenase. bFGF is a heparin binding cytokine that is found inside cells and in extracellular stores bound to heparin or heparin sulfate proteoglycans. bFGF may be released to mediate tissue repair since expression is known to be high in mast cells responding to injury. The monoclonal antibody to bFGF can be used for the study of myometrial smooth muscle cells, uterine leiomyomas, cardiac myocytes, arterial endothelium, gastric carcinoma, and invasive or metastatic melanoma. This antibody stains bFGF in cytoplasm of many diverse cell types.


Clone: bFGF88

Isotype: IgG 2b

Source: Mouse

Immunogen: A unique synthetic peptide of bFGF coupled to keyhole limpet hemocyanin

Specificity: bFGF



Xmatrx: HX031-YCDEAdenocarcinoma stained with Anti-bFGF using AEC chromogen

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Blood Group Antigen Lewis A

Lewis blood group antigens are carbohydrate moieties structurally integrated in mucous secretions. Lewis antigen system alterations have been described in gastric carcinoma and associated lesions. Enhanced expression of Lewis A antigen has been found in malignant transformation of gastric tissues. A panel of antibodies to Lewis antigens, including Lewis A, Lewis B and sialylated Lewis A, is useful in the immunopathological analysis of gastric cancers. Clone 7LE detects Lewis A antigen in cultured cells and tissue sections by immunohistochemistry and reacts with the immunogen in ELISA assays. This antibody stains blood group antigen Lewis A.



Clone: 7LE

Isotype: IgG1

Source: Mouse

Immunogen: Mucin isolated from ovarian cyst fluid

Specificity: Blood Group Antigen Lewis A

Localization: Cytoplasm and Membrane

Pre-treatment: NoneStomach tissue stained with Anti-Blood group antigen Lewis A using AEC chromogen

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Blood Group Antigen Lewis B

Lewis blood group antigens are carbohydrate moieties structurally integrated in mucous secretions. Lewis antigen system alterations have been described in gastric carcinoma and associated lesions. Anomalous expression of Lewis B antigen has been found in some non-secretory gastric carcinomas and colorectal cancers. This antibody will stain Lewis B antigen in formalin-fixed, paraffin-embedded tissues. A panel of antibodies to Lewis antigens, including Lewis A, Lewis B and sialylated Lewis A, is useful in the immunopathological analysis of gastric cancers. Monoclonal antibody 2-25LE detects Lewis B antigen in cultured cells and tissue sections using immunohistochemistry and will react with the immunogen in ELISA assays. This antibody stains blood group antigen Lewis B.



Intestine stained with Anti-Blood group Lewis B antibody using AEC chromogen

Clone: 2-25LE

Isotype: IgG1

Source: Mouse

Immunogen: Mucin isolated from ovarian cyst fluid

Specificity: Blood Group Antigen Lewis B

Localization: Cytoplasm and Membrane

Pre-treatment: None

P

Primary Antibodies


BOB-1

B cell Oct binding protein 1 (BOB-1), is a transcription factor that is required for germinal center formation and immunoglobulin production. Various lymphomas are positive for this marker including the following: B-Chronic lymphocytic leukemia, mantle cell lymphoma, follicular lymphoma, marginal zone lymphoma, plasmacytoma, Burkitt lymphoma, diffuse large cell lymphoma, diffuse large B-cell lymphoma, T-cell rich B-cell lymphoma, nodular lymphocyte predominant Hodgkin lymphoma, whereas classic Hodgkin lymphoma is usually negative.

Tonsil stained with anti-BOB-1 using DAB chromogen

Clone: SP92

Isotype: IgG

Source: Rabbit

Immunogen: Synthetic peptide corresponding to C-terminus of human BOB-1 protein

Specificity: Human BOB-1 protein

Localization: Nucleus


Manual/i6000 HK522-XAK

Xmatrx None

Ready-to-use (Manual): AN731-5ME

AN731-10ME

NU731-UCEConcentrated:

P

Ready-to-use (Automated): i6000TM Xmatrx® AY731-YCDE


FB-731NRecommended Barrier Control:

BRCA1 Protein

The BRCA1 gene was discovered as a region on chromosome 17q21 that has a high frequency of mutation in families predisposed to breast cancer. Specific mutations and variability in expression have been identified and characterized, including the founder mutation 185delAG in Askenazi Jewish families. BRCA1 functions as a tumor suppressor by mechanisms not yet understood. It has recently been suggested that BRCA1 might induce apoptosis similar to the gatekeeper function of the p53 tumor suppressor. This antibody reacts with an epitope mapping near the carboxyl terminus of the normal (non-mutant) BRCA1 gene product.


Concentrated:


PU345-UP

Anti-BRCA1 positivity in recurrent tumor using DAB chromogen

Clone: Polyclonal

Source: Rabbit

Immunogen: A synthetic peptide encompassing a unique epitope within the carboxyl terminal domain of human BRCA1 coupled to Keyhole Limpet Hemocyanin.

Specificity: BRCA1

Localization: Nucleus and Cytoplasm

Pre-treatment: AR Citra/ /EZ-AR 1/EZ-AR 2

Manual: HK087-5KE


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Breast Cancer Antigen BCA-225

This antibody recognizes a 225-250 kD glycoprotein found in most human breast carcinomas and a few other tissues. CU18 does not stain lactating mammary gland.



Clone: CU18

Isotype: IgG1

Source: Mouse

Immunogen: RNA virus-like particles from T47D breast carcinoma cell line (VR).

Specificity: Breast carcinoma Associated Antigen (BCA-225)



Manual/i6000 None

Xmatrx: HX031-YCDE

Breast carcinoma stained with Anti-BCA-225 using AEC chromogen

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AM135-10M AX135-YCD


CA19-9

Carcinoma Antigen 19-9 (CA19-9) is a carbohydrate antigen that reacts specifically with Sialyl Lewis-containing glycolipids and has been isolated and characterized as the oligosaccharide sialylazed lacto-N-fucopentose II antigen. This monoclonal antibody is directed against the CA19-9 antigen, which is expressed in pancreatic carcinomas, hepatobillary carcinomas, the tumor cells of colorectal and gastric cancers.. It can also be found in chronic pancreatitis and in healthy colonic mucosa of patients with colorectal cancer.





Clone: C241:5:1:4

Isotype: IgG1

Source: Mouse

Immunogen: Human colorectal adeno carcinoma cell line COLO205

Specificity: CA19-9 protein


Pre-treatment: AR Citra Plus/ EZ-AR 2

Manual/i6000 HK081-5KE

Xmatrx: HX032-YCDE

Colon stained with Anti-CA19-9 using DAB chromogen

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AM424-10M AX424-YCD


59

Primary Antibodies


CA 125 (Ovarian Tumor Marker)

Monoclonal antibody Ov185:1 reacts with repetitive protein determinant expressed in the protein core of the CA125 human ovarian cancer antigen. This marker is usually associated with ovarian epithelial malignancies. Immunohistochemistry with CA125 antibody in conjunction with other markers was found to be useful in tracing the origin of adeno carcinoma of unknown origin. This antibody stains membrane in ovarian cancer cells.


AM429-10M



Clone: Ov185:1

Isotype: IgG1

Source: Mouse

Immunogen: A partially purified mucin fraction from a pool of cancer tissues from patients with epithelial ovarian cancer.

Specificity: Repetitive protein determinant expressed in the protein core of CA125 human ovarian cancer antigen.

Localization: Membrane/Cytoplasm

Pre-treatment: AR-10/EZ-AR 1/EZ-AR 2



Ovarian carcinoma stained with Anti-Ovarian Tumor Marker (CA125) using DAB chromogen

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Caldesmon

Caldesmon is a smooth muscle and nonmuscle regulatory protein that interacts with actin, myosin, tropomyosin, and calmodulin. Smooth muscle caldesmon is an elongated molecule with a calmodulin, tropomyosin, and actin-binding region at the C-terminus and a myosin-binding domain at the N-terminus. Caldesmon stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, caldesmon inhibits the actomyosin ATPase by binding to F-actin. This inhibition is reduced by calcium-calmodulin and is promoted by tropomyosin.


AN507-10M



Uterus (Endomyo) stained with Anti-Caldesmon using AEC chromogen

Clone: E89

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic phosphor-peptide corresponding to residues surrounding ser789 in the C-terminus of human caldesmon

Specificity: Caldesmon




Xmatrx: HX031-YCDE

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Caldesmon, High MW, Smooth Muscle

Caldesmon is considered to be the marker for smooth muscle cell phenotype. Monoclonal antibody to caldesmon, high molecular weight (120-150kD), in combination with monoclonal antibodies to calponin and smooth muscle myosin heavy chains could be used to distinguish benign and in-situ lesions from invasive carcinomas. Anti-caldesmon antibody may be used to characterize the differentiation process of mammary myoepithelial cells in the developing mammary gland, investigate the nature of myoepithelial cells and study the development of human smooth muscle cells. This antibody stains caldesmon in cytoplasm of vascular, visceral smooth muscle and myoepithelial cells in normal and benign human mammary gland.



Smooth muscle stained with Anti-Caldesmon using DAB

Clone: h-CD

Isotype: IgG1

Source: Mouse

Immunogen: Crude human uterus caldesmon

Specificity: Caldesmon, high molecular weight


Pre-treatment: AR-10/EZ-AR 1

Manual: HK058-5K

Xmatrx: HX031-YCDE

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Calponin

Calponin is a 33 kD thin filament-associated protein that plays a role in regulation of smooth muscle contractility by anchoring myosin to actin. Monoclonal antibody to Calponin in combination with clones SMMS-1(anti-myosin heavy chain antibody) and h-CD (anti-Caldesmon antibody) could be used to distinguish benign and in-situ lesions from invasive carcinomas. This antibody stains Calponin in cytoplasm of vascular and visceral smooth muscle cells, myoepithelial cells in normal and benign human mammary gland, and certain stromal myofibroblasts.


AX333-YCDReady-to-use (Automated): Xmatrx® Concentrated:


Myoepithelial cells in normal breast highlighted by Calponin stained using DAB chromogen

Clone: CALP

Isotype: IgG1

Source: Mouse

Immunogen: Crude human uterus extract

Specificity: Phosphorylated tyrosine



Manual: HK058-5K

Xmatrx: HX031-YCDE

AM333-5M

MU333-UC

FG-333M

P

Primary Antibodies


Calponin-1(C-term)

Calponin is a smooth muscle specific, actin-, tropomysin- and calmodulin-binding protein thought to be involved in regulation of actomyosin as well as the regulation or modulation of contraction. Immuno reactivity for Calponin, along with alpha-smooth muscle actin and smooth muscle myosin heavy chains, confirms the known neoplastic myoepithelial component of adenoid cystic carcinomas and epithelial-myoepithelial carcinomas. The consistently positive staining pattern in adenoid cystic carcinomas may be diagnostically useful in discriminating histologically similar but consistently negative polymorphous low-grade adeno carcinomas.


AN502-10M



Salivary gland tumor (Pleomorphic adenoma) tissue stained with Anti-Calponin-1 (C-term) using AEC chromogen

Clone: EP798Y

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues in the C-terminus of human calponin

Specificity: Calponin


Pre-treatment: AR Citra Plus/EZ-AR 1


Xmatrx: HX031-YCDE

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Calretinin

Calretinin, also know as calbindin 2, is a calcium binding protein that belongs to the calbindin family. It is mainly expressed in the central and peripheral nervous systems and in many normal and pathological tissues. Calretinin can be found in different subsets of neurons and is considered as a valuable marker of neuronal subpopulations for anatomical and developmental studies. It has been implicated as a calcium sensor, and regulator of apoptosis. Calretinin is approved as a highly sensitive and specific marker for mesothelial cells and one of the best positive makers for differentiating epithelial malignant mesotheliomas.


AM583-10M


Appendix stained with Anti-calretinin using DAB chromogen

Clone: 2 E7

Isotype: IgG

Source: Mouse

Immunogen: Recombinant human calretinin

Specificity: Anti-human calretinin




Xmatrx: HX031-YCDE

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Calretinin

Calretinin is approved as a highly sensitive and specific marker for mesothelial cells and one of the best positive markers for differentiating epithelial malignant mesotheliomas. This polyclonal antibody specifically recognizes calretinin in tissue originating from human, monkey, rat and mouse. It does not cross-react with other known calcium-binding proteins as determined by Western Blot analysis and by its distribution in the brain as determined by immunohistochemistry. This antibody stains calretinin antigen in cytoplasm of various neurons in normal brain and mesothelial cells.


AR413-10R

PU413-UPConcentrated:

FG-413PRecommended Positive Control:

Calretinin positivity in brain neurons using AEC chromogen

Clone: Polyclonal

Isotype: N/A

Source: Rabbit

Immunogen: Recombinant human calretinin

Specificity: Calretinin antigen




Xmatrx: HX031-YCDE

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Ready-to-use (Automated): i6000TM Xmatrx® AW413-YCD

Carcinoembryonic Antigen (CEA)

CEA consists of a heterogeneous family of related oncofetal 200 kD glycoproteins that is secreted into the glycocalyx surface of gastrointestinal cells. Usually CEA is demonstrated as a linear labeling of the apical poles of cells lining the glandular lumen and, occasionally, as weak staining near the apex of colonic epithelial cells. Pancreatic carcinomas, testicular tumor, gall bladder neoplasms and granular cell myoblastomas stain positive, whereas malignant tumors of brain, prostate, skin, lymphoreticular tissues, hepatocellular carcinomas, esophageal squamous cell carcinomas, and mesothelioma fail to stain for CEA. This antibody stains carcinoembryonic antigen in the cytoplasm of positive cells.




Colon carcinoma stained with Anti-CEA using AEC chromogen

Clone: B01-94-11M-P

Isotype: IgG 2b

Source: Mouse

Immunogen: Human carcinoembryonic antigen

Specificity: Carcinoembryonic antigen (CEA)


Pre-treatment: None

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AM009-10M AX009-YCD


61

Primary Antibodies



CEA is demonstrated as a linear labeling of the apical poles of cells lining the glandular lumen and, occasionally, as weak staining near the apex of colonic epithelial cells. CEA, however, should not be used as a marker of differentiation because many colon and lung tumors actually show increased staining with differentiation. Pancreatic carcinomas, testicular tumor, gall bladder neoplasms and granular cell myoblastomas stain positive, whereas malignant tumors of brain, prostate, skin, lymphoreticular tissues, hepatocellular carcinomas, oesophageal squamous cell carcinomas, and mesothelioma fail to stain for CEA.





Clone: CEA88

Isotype: IgG1

Source: Mouse

Immunogen: Partially purified human CEA



Pre-treatment: None

Colon showing CEA positivity stained using DAB chromogen

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AM365-10M AX365-YCD



CEA consists of a heterogeneous family of related oncofetal 200 kD glycoproteins that is secreted into the glycocalyx surface of gastrointestinal cells. Usually CEA is demonstrated as a linear labeling of the apical poles of cells lining the glandular lumen and, occasionally, as weak staining near the apex of colonic epithelial cells. Pancreatic carcinomas, testicular tumor, gall bladder neoplasms and granular cell myoblastomas stain positive, whereas malignant tumors of brain, prostate, skin, lymphoreticular tissues, hepatocellular carcinomas, esophageal squamous cell carcinomas, and mesothelioma fail to stain for CEA. This antibody stains carcinoembryonic antigen in the cytoplasm of the positive cells.

Clone: Polyclonal

Isotype: N/A

Source: Rabbit

Immunogen: CEA isolated from secondary colon carcinoma by salt precipitation, ion and gel chromatography




Xmatrx: HX031-YCDE

CEA expression in hepatocellular carcinoma stained using DAB chromogen

AR009-5R

FG-009M



AR009-5R

FG-009PAW009-YCD


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Catenin (p120)

Catenins are proteins that are linked to the cytoplasmic domain of transmembrane cadherins. Catenin (p120) is one member of this Armadillo gene family of junctional plaque proteins. The association of catenins to cadherins produces a complex which is linked to the actin filament network, and which seems to be important for cadherins cell-adhesion properties. Recently it became clear that some catenins also mediate nuclear signaling. Catenin (p120) is implicated both in cell transformation by SRC and in ligand-induced receptor signaling through the EGF, PDGF, CSF-1 and ERBB2 receptors.


AN523-10M



Breast carcinoma stained with Anti-p120 Catenin using DAB chromogen

Clone: YE372

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues near N- terminusof of human Catenin (p120) was used as an immunogen.

Specificity: Catenin (p120)

Localization: Cytoplasm/Membrane




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Catenin Delta 1 (p120)

Catenins are proteins that are linked to the cytoplasmic domain of transmembrane cadherins. Catenin (p120) is a memberof this Aemadillo gene family of junctional plaque proteins. Cytoplasmic accumulation of Catenin (p120) has been observed in lung cancer, pancreatic cancer, gastric cancer and colon cancers and is associated with poor prognosis in colon cancer patients. In breast lobular neoplasia, anti p120 Catenin shows a diffuse cytoplasmic immunostaining pattern, while breast ductal neoplasma retains the membrane immunostaining pattern. Catenin (p120) antibody is useful in differentiation of lobular carcinoma from ductal carcinoma of the breast and in identifying early lesions of lobular neoplasia.



FB-706PRecommended Barrier Control:


Breast Ca. stained with anti-Catenin delta 1 (p120) antibody using DAB chromogen

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AR706-10R AW706-YCD


Clone: Polyclonal

Isotype: IgG

Source: Rabbit

Immunogen: Catenin delta

Specificity: Catenin delta

Localization: Membrane and cytoplasm

Pre-treatment: Citra Plus/EZ-AR 1


Xmatrx: HX031-YCDE

Primary Antibodies


Cathepsin D

Cathepsin D production and secretion appears to be induced by estrogen in estrogen-responsive tumor cells but is constitutively produced in estrogen-unresponsive tumor cells. Immunohistochemical localization of Cathepsin D in normal human tissues has shown a granular cytoplasmic staining pattern corresponding to intracellular lysosomes. Among normal tissues studied, highest concentrations of Cathepsin D were found in sweat glands and liver with some staining of sebaceous glands.

Breast carcinoma stained with Anti-Cathepsin D using AEC chromogen

Clone: C15

Isotype: IgG2b

Source: Mouse

Immunogen: Cathepsin D protein purified from human spleen

Specificity: Cathepsin D



Xmatrx: HX031-YCDE

P




CD1a

CD1 is expressed on cortical thymocytes, Langerhans cells, and dendritic cells. It is absent on mature peripheral blood T cells but intracytoplasmic expression is detected on activated T lymphocytes.At least five CD1 genes (CD1a, b, c, d, and e) are identified. CD1 proteins have been demonstrated to restrict T-cell response to non-peptide lipid and lycolipid antigens and play a role in non-classical antigen presentation. Ab-5 detects cortical thymocytes, Langerhans cells in epidermis, dendritic cells of dermis and Langherhans cells of mucosa of tonsil. It may also detect small focal groups of lymphocytes outside the germinal centers of tonsil indicating a crossreaction with CD1b. This antibody is useful in the characterization of leukemias and lymphomas.


AM490-10M



Lymph node stained with Anti-CD1a using DAB as Chromogen

Clone: O10

Isotype: IgG

Source: Mouse

Immunogen: Human CD1a

Specificity: CD1a





P


CD2

The CD2 antigen, also known as lymphocyte function antigen2 (LFA2), is a single chain type I transmembrane molecule of about 50 kD and consists of 351 amino acids. It plays a critical role in activation of T cells. It binds to CD58 on antigen presenting cells and induces tyrosine phosphorylation of other molecules involved in T cell activation. It also plays a regulatory role in T-cell or NK-cell mediated cytolysis. CD2 antigen is expressed on a majority of T cells in peripheral lymphoid tissue, NK cells, cortical thymocytes and most malignant cells of T cell origin. This antibody stains membrane of positive T cells.




Tonsil stained with Anti-CD2 using DAB chromogen

Clone: AB75

Isotype: IgG1 kappa

Source: Mouse

Immunogen: Recombinant fusion protein corresponding to the external domain of the CD2 molecule.

Specificity: CD2 antigen (LFA-2)


Pre-treatment: AR-10/EZ-AR2

Manual: HK058-5K

Xmatrx: HX032-YCDE

P


CD3 Epsilon

CD3 (Cluster of Differentiation 3) is a complex of proteins that associates directly with the T cell antigen receptor (TCR). Antigen binding to the TCR leads to IL-2 secretion via activation of a tyrosine phosphorylation pathway and a phospholipase C (PLC) pathway, in turn activating protein kinase C. CD3 is composed of five invariant polypeptide chains that associate to form three dimers. The five invariant chains of CD3 are labelled gamma, delta, epsilon, zeta and eta. The gamma, epsilon and delta chains each contain a single copy of a conserved immunoreceptor tyrosine-based activation motif (ITAM). Phosphorylated ITAMS act as docking sites for protein kinases such as ZAP-70 and Syk.


AN477-10M



Spleen tissue stained with Anti-CD3 epsilon using DAB chromogen

Clone: EP449E

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues in cytoplasmic domain of human CD3 epsilon.

Specificity: CD3 epsilon




Xmatrx: HX032-YCDE

P


63

Primary Antibodies


CD3 (T Cell)

CD3 is a lineage-specific “pan T-cell” surface antigen composed of five different polypeptide chains with molecular masses ranging from 16 to 28 kD. Antibody UCHT1 reacts with the 20 kD epsilon chain of the CD3 molecule. CD3 is normally present on mature thymocytes, resting and activated peripheral T lymphocytes (both inducer and suppressor/cytotoxic), and on some natural killer cells. It is absent in peripheral B lymphocytes, monocytes, granulocytes, and platelets. This antibody stains CD3 antigen in the cytoplasm of immature and common thymocytes and on the surface of mature thymocytes in frozen tissue sections.



Frozen tonsil stained with Anti-CD3 using DAB chromogen

Clone: UCHT1

Isotype: IgG1 Kappa

Source: Mouse

Immunogen: Human infant thymocytes and peripheral lymphocytes from a patient with Sezary cell leukemia

Specificity: CD3



Xmatrx: HX031-YCDE

F


CD3 (T Cell)

Human CD3 complex is associated with the T cell receptor (TcR) at the cell surface. Expression of CD3 antigen is generally restricted to the T-cell lineage, but weak expression might also occur in Purkinje cells in the brain, in macrophages, and in Reed-Sternberg cells in Hodgkin's lymphoma. The CD3 antigen is expressed early in the maturation of T cells. Monoclonal antibody PS1 reacts with the non-glycosylated epsilon chain of CD3. The antibody stains CD3 antigen in the membrane of the positive cells.


Concentrated:



Clone: PS1

Isotype: IgG 2a

Source: Mouse

Immunogen: Fusion protein to the epsilon chain of CD3

Specificity: CD3 antigen




Xmatrx: HX031-YCDE

AM322-5M

MU322-UC

FG-322M

P

AM322-10M AX322-YCD



CD4

CD4 is a 55-60 kD cell-surface glycoprotein, which participates in the molecular complexes involved in both T cell development and its antigen recognizing activity, by binding to the nonpolymorphic region of class II MHC. CD4 is considered as a stage marker of T cell development in the thymus, for it is expressed on the cell surface in a stage specific manner, during T cell development. This antibody reacts on a low level with human monocytes and macrophages but does not react with B-cells, granulocytes and thrombocytes. This antibody stains CD4 antigen on the membrane of positive T lymphocytes.




Clone: 4B12

Isotype: IgG1

Source: Mouse

Immunogen: Prokaryotic recombinant protein corresponding to the external domain of the CD4 molecule

Specificity: CD4 protein




Xmatrx: HX031-YCDE



P

AM421-10M AX421-YCD


CD4

CD4 (cluster of differentiation 4) is a glycoprotein found on the surface of immune cells such as T helper cells, monocytes, macrophages and dendritic cells. CD4 is expressed in the majority of T-cell lymphomas, including mycosis fungoides. Lymphomas are CD4 positive with the exception of aggressive NK-cell leukemia and extranodal NK/T-cell lymphoma. CD4 plays an important role in the classification of lymphocytes in inflammatory lesions and malignant lymphomas.

Tonsil stained with anti-CD4 using DAB chromogen

Clone: EP204

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues of human CD4 protein




Manual/i6000 HK058-5K

Xmatrx HX032-YCDE


AN722-10M



P

Ready-to-use (Automated): i6000TM Xmatrx® AY722-YCD


Primary Antibodies


CD5

The CD5 antigen, also known as T1, is a 67 kD single chain glycoprotein expressed on normal and malignant T cells and on chronic lymphocytic leukemia cells. It is found in high density on medullary thymocytes and in low density on cortical thymocytes.




Clone: 4C7

Isotype: IgG1 Kappa

Source: Mouse

Immunogen: Recombinant protein corresponding to the external domain of the CD5 molecule.

Specificity: Human CD5 antigen, 67 kD antigen




Xmatrx: HX032-YCDE

Tonsil tissue stained with Anti-CD5 using DAB chromogen

P

AM430-10M Ready-to-use (Automated): i6000TM Xmatrx® AX430-YCD

CD5

CD5 (Lymphocyte antigen T1/Leu-1) is a transmembrane glycoprotein which has been implicated as a receptor in the regulation of T-cell proliferation. CD5 is part of the scavenger receptor cysteine-rich (SRCR) family group B and it is found on 95% of thymocytes and 72% of peripheral blood lymphocytes. In lymph nodes, the main reactivity is observed in T cell areas. CD5 is expressed by T cell leukemia, lymphomas, and activated T cells. Occasionally, CD5 antigen is also expressed on a subset of B cells. Mantle cell lymphomas (same as diffuse centrocytic lymphomas) are CD5+ while the follicle center cell lymphomas are CD5. A ligand for CD72/LYB-2, CD5 has been linked to negative regulation of T cell and B cell receptors signaling.


AN512-10M



Lymph node stained with Anti-CD5 using AEC chromogen

Clone: EP2952

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues in human CD5

Specificity: CD5





P


CD7

The CD7 molecule is membrane-bound glycoprotein of 40kD and is the earliest T Cell specific antigen to be expressed in lymphocyetes. CD7 antigen is also the only early marker to persist throughout differentiation. The function and role of the CD7 molecule has not yet been fully identified although the activation of T cells with gamma/ delta receptors has been proposed based on mAb- activation. CD7 antigen is reported to be found on a majority of peripheral blood T cells, most natural killer cells and thymocytes.


Tonsil stained with Anti-CD7 using DAB Chromogen

P

AM702-10M AY702-YCD


Clone: LP15

Isotype: IgG2b

Source: Mouse

Immunogen: CD7

Specificity: CD7




Xmatrx: HX031-YCDE




CD8

The CD8 antigen is composed of two chains and has a molecular weight of 32kD. This antibody identified cytotoxic/suppressor T cells that interact with binding to the nonpolymorphic region of class I MHC.CD8 is a stage marker of T cell development in the thymus, and is expressed on mature peripheral T cells, most cytotoxic T cells, CD4/CD8+ thymocytes, NK cells and cortical thymocytes. This monoclonal antibody is directed against the CD8 antigen, which is expressed on human T lymphocytes. It does not react with B-cells, granulocytes and thrombocytes. This antibody stains CD8 antigen on the membrane of positive T lymphocytes.

Tonsil tissue stained with Anti-CD8 using DAB chromogen

Clone: 1A5

Isotype: IgG1

Source: Mouse

Immunogen: Prokaryotic recombinant protein corresponding to the external domain of the CD8 molecule.





Xmatrx: HX032-YCDE

P

AM422-5M

MU422-UC

FG-422M

FB-422M

AM422-10M


Concentrated:




65

Primary Antibodies


CD8

The CD8 molecule consists of two chains, α and β, which are expressed as a disulphide-linked α/β heterodimer or as an α/α homodimer on a T cell subset (25-35% of mature peripheral T-cells), thymocytes (70-80%), and NK cells (30%, which are also CD3 negative). The majority of CD8+ T cells express CD8 as α/β heterodimer. CD8 functions as a coreceptor in concert with TCR for binding the MHC class I/peptide complex. The HIV-2 envelope glycoprotein binds CD8 α chain (but not β chain).

Tonsil stained with anti-CD8 using DAB

Clone: SP16

Isotype: IgG

Source: Rabbit

Immunogen: Synthetic peptide corresponding to C-terminus of alpha chain of the human CD8 molecule

Specificity: CD8




Xmatrx: HX032-YCDE


AN740-10M


P


CD8 (C-term)

The T cell coreceptor CD8 is a cell-surface glycoprotein expressed either as a disulfide-linked homodimer of two CD8 alpha monomers, or a heterodimer of CD8 alpha and CD8 beta. These receptors interact with ligands, such as major histocompatibility complex (MHC) class I, on the outside of the cell, with proteins inside the cell, such as the tyrosine kinase p56lck, and possibly with proteins on the same cell-surface. CD8 plays an important role in T cell development in the thymus and T cell activation in the periphery.


AN513-10M




Clone: EP1150Y

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues near the C- terminus of human CD8.

Specificity: CD8 ( C-term)





P


CD8 (T cell, Suppressor/Cytotoxic)

The CD8 antigen, also known as T8 or Leu2 or Lyt2 or T cell co-receptor, is a dimer with a molecular mass of 32 kD. The T8 antigen is expressed by the suppressor/cytotoxic subset of T lymphocytes which comprise most of the cortical thymocytes and approximately 30% of peripheral blood T cells. Studies have demonstrated that increased levels of T8+ cells are associated with viral infections such as hepatitis B, Epstein-Barr, and cytomegalovirus. This antibody may be used in the study of cell-mediated cytotoxicity and that of immunoregulation and T-lymphocyte-mediated suppression. This antibody stains CD8 (T8) antigen suppressor/cytotoxic T lymphocytes and majority of thymocytes (approximately 80%) in frozen tissue sections.

Frozen tonsil stained with Anti-CD8 using AEC chromogen

Clone: T8

Isotype: IgG1 Kappa

Source: Mouse

Immunogen: Human thymocytes





F




CD10

CD10, a 100KD glycoprotein, also known as Common Acute Lymphocytic Leukemia Antigen (CALLA), is a cell surface enzyme with neutral metalloendopeptidase activity which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt’s and follicular germinal center lymphomas, and chronic myelogenous leukemia (CML). It is also expressed on the surface of normal early lymphoid progenitor cells, immature B cells within bone marrow and germinal center B cells within lymphoid tissue.CD10 is also present on breast myoepithelial cells, with especially high expression on the brush border of kidney and gut epithelial cells.

CD10 expression in kidney stained using DAB chromogen

Clone: 56C6

Isotype: IgG1

Source: Mouse

Immunogen: Human CD10

Specificity: CD10


Pre-treatment: EZ-ARTM 1//EZ-AR 2




Concentrated:


AM451-5M

MU451-UC

FG-451M

P


Primary Antibodies


CD11b (C3bi receptor)

CD11b also known as M01, a human myeloid antigen, is a noncovalently associated, two-subunit glycoprotein of 94 and 155 kD. This antibody can be used as a neutrophil marker. It identifies the C3bi receptor and is expressed by peripheral blood monocytes, certain macrophages, granulocytic cells (from myelocytes to mature neutrophils) and a subset of null cells in frozen tissue sections. Histiocytic cells in the spleen and lymph nodes also express this antigen.


Frozen tonsil mucosa stained with Anti-CD11b using DAB chromogen

Clone: M01

Isotype: IgM kappa

Source: Mouse

Immunogen: Human adherent mononuclear cells

Specificity: Myeloid antigen M01




F


CD11b/ITAM

Integrin alpha-M (ITAM, ITGAM, CD11b, MAC-1 alpha subunit, CR3 alpha chain) belongs to the integrin alpha chain family and it is predominately presented in human myeloid cells, NK1 cells, monocytes, and granulocytes. The alpha subunit of ITAM/beta-2 complex (CD11b/CD18, Mac-1), is a receptor for fibrinogen (binding to P1 and P1 peptides of gamma chain), factor X, and ICAM1. ITAM/beta-2 is implicated in adhesive interactions of monocytes, macrophages, and granulocytes. It is also linked to mediation of complement-coated particle uptake.


AN546-10M


Spleen tissue stained with Anti-CD11bITAM using DAB as chromogen

Clone: EPR1344

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues in human CD11b .

Specificity: CD11b/ITAM




Xmatrx: HX031-YCDE


P


CD11C

CD11c (integrin alpha chain protein) combines with the beta 2 chain to form a leukocyte specific integrin. The integrin CD11c/CD18 functions as a cell surface receptor for numerous soluble factors and proteins (LPS, fibrinogen, iC3b), mediates leukocyte interactions with other cell types and is a signal transducing receptor. CD11c/CD18 is found primarily on myeloid cells, where its expression is regulated both during differentiation and during monocyte maturation into tissue macrophages. The CD11c antigen is a useful marker for hairy cell leukemia and in its differentiation from other leukemias and lymphomas.


AN555-10M


Tonsil tissue stained with Anti-CD11c using DAB as chromogen

Clone: EP1347Y

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues onthe C-terminus of human CD11c/ITGAX

Specificity: CD11c


Pre-treatment: AR Citra Plus/ /EZ-AR 1/EZ-AR 2




P


CD14

CD14 is a glycophosphatidyl inositol-anchored protein expressed on the surfaces of monocytes and polymorphonuclear leukocytes. It acts as a co-receptor for the detection of bacterial lipopolysaccharide (LPS). CD14 cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial LPS. It acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. CD14 is expressed strongly on peripheral blood monocytes and macrophages, and weakly on the surface of granulocytes.


AN515-10M



Placental tissue stained with Anti-CD14 using AEC chromogen

Clone: EPR3653

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues in human CD14

Specificity: CD14




Xmatrx: HX031-YCDE

P


67

Primary Antibodies


CD15 (Blood Group Antigen Lewis X)

CD15 (BRA4F1) reacts with human CD15 antigen present on myeloid cells, mainly granulocytes but not on B cells, T cells, monocytes, erythrocytes or platelets. It also reacts with Hodgkin's and Reed-Sternberg cells in individuals with Hodgkin's disease. This antibody stains CD15 antigen in positive cells.





Clone: BRA4F1

Isotype: IgM

Source: Mouse

Immunogen: Myelomonocytic leukemia cells

Specificity: CD15

Localization: Membrane/Cytoplasm/perinuclear structures

Pre-treatment: None

Bone marrow stained with Anti-CD15 using DAB chromogen

P


AM437-5M

MU437-UC

FG-437M

AX437-YCD

CD16

CD16 antigen is also known as Fc gamma receptor III and has a molecular weight of 50 to 70kD. It is a low affinity Fc receptor for complexed IgG-Fc gamma RIII, expressed on natural killer (NK) cells, granulocytes, activated macrophages and a subset of T cells expressing alpha-beta or gamma-delta T cell antigen receptors. Antibody-dependent cytotoxicity of NK cells is triggered by the engagement of CD16 with the Fc portion of IgG immunoglobulins bound to target cell-associated antigens. This antibody may be useful in the study of NK cell activity in autoimmune, neoplastic and infectious diseases. This antibody stains membrane and in the cytoplasm of positive cells.


Concentrated:


Clone: 2H7

Isotype: IgG2a

Source: Mouse

Immunogen: Recombinant fusion protein corresponding to the external domain of the CD16 molecule common to both the trans-membrane form and the GPI-linked form


Localization: Membrane & Cytoplasm


Manual: HK058-5K

Xmatrx: HX032-YCDE


P


CD19

CD19 is expressed on follicular dendritic cells and B cells. In fact, it is present on B cells from earliest recognizable B-lineage cells during development to B-cell blasts but is lost on maturation to plasma cells. It primarily acts as a B cell co-receptor in conjunction with CD21 and CD81. CD19 has been observed in lymphomas and leukemias but often weak/negative in follicular lymphoma or diffuse large B-cell lymphoma. CD19 may provide useful diagnostic information for the study of B-lymphoproliferative disorders.


Clone: EP169

Isotype: IgG

Source: Rabbit

Immunogen: A recombinant fragment corresponding to residues in human CD19 protein

Specificity: Human CD19 protein




Xmatrx: HX031-YCDE


AN729-10M



P



CD20

CD20 is a transmembrane, non-glycosylated protein expressed on B-cell precursors and mature B cells, but is lost following differentiation into plasma cells. This antibody does not cross-react with non-hematopoietic neoplasms. CD20 (B-cell Pan) reacts with a membrane antigen present in B-cells. This antibody strongly recognizes Reed-Sternberg cells predominant in Hodgkin`s disease. Since no staining of histiocytes or plasma cells has been observed and CD20 has not been detected in T-cell malignancies, it is a very strong marker of B-cell lymphomas. B-cell panmarker recognizes a formalin resistant intracytoplasmic antigen.


AM537-10M




Clone: CD20/C23

Isotype: IgG1 kappa

Source: Mouse


Specificity: CD20


Pre-treatment: AR Citra Plus/ /EZ-AR 1/EZ-AR 2


Xmatrx HX031-YCDE/HX032-YCDE

FG-537M

P


Primary Antibodies


CD20 (B Cell)

This antibody reacts with a formalin-resistant intracytoplasmic epitope found in the majority of B cells which is now considered to be the CD20 antigen, a pan-B cell marker. The antibody primarily recognizes a 33 kD polypeptide B cell component and also a minor 30 kD cellular antigen.The staining pattern is consistent with pan-B reactivity, producing staining for B cells in lymphoid and peripheral blood tissue. This antibody intensely stains germinal centers and B immunoblasts in lymphoid tissue. L26 may prove to be a useful marker for L&H variants of Reed-Sternberg cells of Hodgkin's lymphomas where reactive pattern is distinct from other Reed-Sternberg variants. This antibody stains positive for membrane and some cytoplasm for B cells.


Concentrated:



Tonsil tissue stained with Anti-CD-20 using DAB chromogen

Clone: L-26

Isotype: IgG2a Kappa

Source: Mouse

Immunogen: Human tonsil B cells

Specificity: CD20


Pre-treatment: None

AM238-5M

MU238A-UC

FG-238M

FB-238M

P


CD20 (B Cell)

The CD20 antigen, also known as B1, is a 33-35 kD glycoprotein found on all B cells in peripheral blood, lymph node, spleen, tonsil, and bone marrow. It is expressed in 50% of CALLA-positive acute lymphoblastic leukemias (ALL), but not on normal T lymphocytes, monocytes, granulocytes, or tumors derived from these cell lineages. Aside from B cells, other normal cells do not express the CD20 antigen.This antibody may be used in the study of normal and neoplastic cells of B origin, in the study of B cell development and activation, and in the classification of acute leukemias. This antibody stains CD20 (B1) antigen on membranes of all B cells in peripheral blood, lymph node, spleen, tonsil, and bone marrow in frozen tissue sections.


Concentrated:


Clone: B1

Isotype: IgG2 Kappa

Source: Mouse

Immunogen: Human B cell ascites Burkitt's lymphoma cells

Specificity: B1+ B cells




AM265-5M

MU265-UC

F


CD21

CD21 is a transmembrane protein that serves as the complement receptor C3d and the Epstein-Barr virus, also known as CR2. CD21 is found on human B cells. CD21 interacts with the p53 anti-oncoprotein, the p68 calcium binding protein and the nuclear p120 ribonucleoprotein to regulate their functions. Phosphorylation of CD21 inhibits its interaction with the nuclear p120 ribonucleoprotein. The soluble CD21 (sCD21) was shown to efficiently trigger CD23 signaling pathways in human monocytes. By inducing release of proinflammatory cytokines and upregulating expression of molecules involved in antigen presentation, sCD21 modulates critical monocyte functions that may be relevant to allergic and inflammatory disorders.


AN497-10M


Tonsil tissue stained with Anti-CD21 using AEC chromogen

Clone: EP3093

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues on the C terminus of human CD21.

Specificity: CD21





P



CD21 (B Cell)

The B2 antigen, also known as CD21 and CR2, is a 140 kD glycosylated Type 1 integral membrane protein. The CD21 molecule is expressed on mature B lymphocytes, follicular dendritic reticulum cells (FDC), pharyngeal epithelial cells, and possibly on a subset of normal thymocytes. This antigen is also expressed by B lymphocytes in patients with B cell lymphomas, most B cell chronic lymphocytic leukemia (CLL), and a small portion of non-T cell acute lymphoblastic leukemias (ALL). This antibody stains B2 (CD21) antigen in membranes of mature B lymphocytes, follicular dendritic reticulum cells (FDC), pharyngeal epithelial cells, and possibly on a subset of normal thymocytes in frozen tissue sections.



Clone: B2

Isotype: IgM Kappa

Source: Mouse

Immunogen: Human diffuse poorly differentiated lymphoma cells from a patient with B cell lymphoma

Specificity: B2+ B lymphocytes




F


69

Primary Antibodies


CD22

The CD22 antigen also known as BL-CAM is a single chain type I transmembrane molecule which contains seven Ig-like domains and molecular weight of 130 to 140kD. In B-cell malignancies, CD22 expression ranges from 60% to 80% depending on the histological type and on the assays used. CD22 antigen is weakly expressed in myeloid leukemias and non-T cell acute lymphoblastic leukemias and is strongly expressed in hairy cell leukemias. It is absent on peripheral blood T cells, T cell leukemias, granulocytes, and monocytes. This antibody stains both membrane and cytoplasm of B lymphocytes.





Clone: FPC1

Isotype: IgG1

Source: Mouse

Immunogen: Recombinant fusion protein corresponding to the external domain of the CD22 molecule

Specificity: Human CD22 antigen (BL-CAM)



Manual: HK058-5K

Xmatrx: HX031-YCDE

P

AX439-YCD Ready-to-use (Automated): Xmatrx®

CD23

CD23 is a 45 kD type II integral membrane glycoprotein that belongs to the C-type lectin family of adhesion molecules.The CD23 molecule is identical to the low affinity IgE receptor found on B-cells. CD23 has been proposed to be an important regulator of IgE synthesis. Anti-CD23 antibody treatment of rats inhibited antigen-specific IgE immune response by 90%. CD23 is a common B cell/monocyte surface antigen. CD23 is expressed on IgM+/IgD+ B cells, as well as on a variety of other cells, including monocytes, eosinophils, dendritic cells, platelets, and macrophages. Expression of CD23 has been detected in neoplastic cells such as chronic lymphocytic leukemia, some cases of lymphoma and is strongly expressed on EBV transformed B lymphoblasts.


Concentrated:Recommended Positive Control:

AR460-5R

PU460-UP

FG-460P

Clone: Polyclonal

Isotype: IgG

Source: Rabbit

Immunogen: CD23 antigen

Specificity: CD23




Xmatrx: HX031-YCDECD23 positivity in Lymph node stained using DAB chromogen

P

AR460-10R Ready-to-use (Automated): i6000TM Xmatrx® AW460-YCD

CD25

CD25 antigen is an alpha subunit of the interleukin-2 receptor. It is expressed on a subpopulation of T cells subsequent to their activation with an antigen or mitogen in the presence of interleukin-1. CD25 is also found on HTLV-1 transformed T and B cells, EBV transformed B cells, myeloid precursors and oligodendrocytes. This antibody may be useful in studies of inflammation, lymphoid neoplasia, autoimmune diseases or allograft rejection to help characterize CD25 expression.


Clone: 4C9

Isotype: IgG1

Source: Mouse


Specificity: CD25


Pre-treatment: EZ-ARTM 1/EZ-AR 2




Concentrated:


AM453-5M

MU453-UC

FG-453M

P


CD29 (Integrin Beta-1 Subunit)

Integrins play an important role in cell adhesion and migration, and their normal function is critical in the induction and maintenance of cell differentiation. This antibody reacts with CD29, the 130 kD integrin ß1 subunit. CD29 is ubiquitous, with broad tissue distribution, but is not expressed on erythrocytes and is expressed only weakly on granulocytes. Loss or down-regulation of CD29 has been proposed to be one of the general pathways through which carcinoma cells may acquire a more invasive and differentiated phenotype. This antibody stains CD29 antigen in cell membrane of most cells including all leukocytes, although very weak on granulocytes.




Breast carcinoma expressing CD 29 stained using DAB chromogen

Clone: JB1a

Isotype: IgG

Source: Mouse

Immunogen: Purified ß 1 integrin from Jurkat cells

Specificity: CD29



Manual HK087-5KE

Xmatrx: HX032-YCDE

P


Primary Antibodies


CD30 (Ki-1 Antigen)

CD30 (Ki-1 antigen), a 120 kD single chain glycoprotein, is expressed in only a small population of normal lymphoid tissue. By contrast, it is expressed in approximately 50% of all malignant lymphomas including all cases of Hodgkin's disease and a vast majority of Ki-1 positive anaplastic large cell lymphomas. Ki-1 antigen can be detected in sera from lymphoma patients, but not in sera from normal individuals with systemic infection. This antibody stains CD30 (Ki-1) antigen in the membrane of positive cells.


Hodgkins stained with Anti-CD30 using AEC chromogen

Clone: Ber-H2

Isotype: IgG1 Kappa

Source: Mouse

Immunogen: Hodgkin's lymphoma cell line L428

Specificity: CD30 (Ki-1) antigen




Xmatrx: HX031-YCDE

P



CD30 (Ki-1 Antigen)

CD30 (Ki-1 antigen), a 120 kD single chain glycoprotein, is expressed in only a small population of normal lymphoid tissue. By contrast, it is expressed in approximately 50% of all malignant lymphomas including all cases of Hodgkin's disease and a vast majority of Ki-1 positive anaplastic large cell lymphomas. Ki-1 antigen can be detected in sera from lymphoma patients, but not in sera from normal individuals with systemic infection. This antibody stains CD30 antigen in membrane and sometimes cytoplasm of the positive cells.


Concentrated:

Reed Sternberg cell showing perinuclear dot positivity of CD30 antibody stained using DAB chromogen

Clone: HRS-4

Isotype: IgG1

Source: Mouse

Immunogen: Cell line L540, derived from Hodgkin's disease

Specificity: CD30 (Ki-1 antigen)

Localization: Membrane (mostly perinuclear dot positivity) & Cytoplasm



Xmatrx: HX031-YCDE

AM351-5M

MU351-UC

P


CD31 (Endothelial Cell)

Anti-CD31 monoclonal antibody JC/70A reacts with a membrane glycoprotein with an apparent size of 100 kD in endothelial cells and 130 kD in platelets. It strongly stains endothelium in normal tissue as well as benign and malignant tumor tissue. The antibody labels mega-karyocytes, platelets, and occasionally plasma cells, and weakly stains mantle zone B cells, peripheral T cells and neutrophils. This antibody stains CD31 antigen in membrane and sometimes cytoplasm of endothelial and other positive cells in normal and abnormal tissues.


Endothelial cells stained with Anti-CD31using DAB chromogen

Clone: JC/70A

Isotype: IgG1 Kappa

Source: Mouse

Immunogen: Spleen membrane from a patient with hairy cell leukemia



Pre-treatment: Pepsin, 370C for 5 min./EZ-AR 2

Manual: EK000-5K

Xmatrx: HX031-YCDE

P



CD31 (PECAM-1)

CD31 (Platelet Endothelial Cell Adhesion Molecule) is a 145 kD cell surface glycoprotein that was originally defined by a monoclonal antibody which is bound to endothelial cells and also to platelets. This protein may be a component involved in the interaction of endothelial cells with coagulation factors, platelets, and the subendothelial matrix. The antibody has been shown to be specific for CD31 and reacts mainly with platelets, monocytes, macrophages, granulocytes, and B cells. The other reactive cells are endothelial cells, histiocytes, and glomeruli. This antibody stains CD31 antigen in membrane of endothelial cells and other positive cells.




Lymph node stained with Anti-PECAM-1 using AEC chromogen

Clone: 9G11

Isotype: IgG1

Source: Mouse

Immunogen: Activated human umbilical vein endothelial cells

Specificity: CD31



Manual: HK058-5K


P


71

Primary Antibodies


CD34 (Endothelial Cell)

This is an antibody to the CD34 antigen in human endothelial and hematopoietic cells. It stains positive in a variety of vascular and lymphatic tumors. QBEnd/10 may now prove to be a more specific method of evaluating vascularization than Factor VIII antibody and is an important tool for tumor evaluation. This antibody stains endothelial cell cytoplasm and cross-reacts with basement membrane collagen.





Endothelial cells stained with Anti-CD34 using DAB chromogen

Clone: QBEnd/10

Isotype: IgG1

Source: Mouse

Immunogen: CD34 isolated from human placental endothelial cells

Specificity: CD34


Pre-treatment: None

P

AM236-10M AX236-YCD


CD35

The CD35 antigen is a transmembrane monomeric glycoprotein of 60-250kD. It is also known as complement receptor 1 (CR1) or C3b/C4b receptor as it binds the complement components C3b and C4b and thereby helps clear foreign particles. By facilitating C3b and C4b cleavage by factor I and accelerating the decay of the C3 and C5 convertases, CD35 limits complement activation and produces ligands for other complement receptors. CD35 antigen has been used in characterization of tumors of histiocytes and accessory dendritic cells by immunohistochemistry. This antibody stains the membrane of follicular dendritic cells, a subset of T- cells.





Clone: RLB25

Isotype: IgG2b

Source: Mouse

Immunogen: Prokaryotic recombinant fusion protein corresponding to the first four complement control protein domains of the CD35 molecule

Specificity: CD35





P


CD35

CD35, also named as erythrocyte complement receptor 1 (CR1), is a member of the complement activation (RCA) family and is located in the ‘cluster RCA’ region of chromosome 1. CD35 expressed by glomerular podocytes, erythrocytes, and leukocytes (B cells, subset of T cells, monocytes, macrophages, neutrophils, and eosinophils). CD35 also can be detected on follicular dendritic cells. It is a marker for the diagnosis of follicular dendritic cell sarcoma. This antibody labels dendritic cells in tonsil and spleen and glomerular podocytes in kidney.

Tonsil stained with anti-CD35 using DAB

Clone: SP191

Isotype: IgG

Source: Rabbit

Immunogen: Synthetic peptide near from the C-terminus of human CD35 protein

Specificity: CD35




AN741-10M


P


CD41/Integrin Alpha IIb

Integrin alpha chain 2b, also known as CD41, is a heterodimeric integral membrane protein. CD41 is expressed on platelets and megakaryocytes, but also on early embryonic hematopoietic stem cells. The integrin alpha chain associates with a beta 3 chain, CD61. The resulting CD41/CD61 complex is a receptor for fibronectin, fibrinogen, von Willebrand factor, vitronectin and thrombospondin, and has a crucial role in coagulation. Mutations that impair its role in coagulation result in thrombasthenia.

Spleen stained with anti-CD41 using DAB chromogen

Clone: EP178

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues of human CD41/Integrin alpha IIb protein

Specificity: CD41/Integrin alpha IIb




Xmatrx None


AN732-10ME


P




Primary Antibodies


CD43 & CD45RA Cocktail

MB1 recognizes a membrane-bound antigen which occurs on all B cells with the exception of plasma cells, and on some mature T cells. The MB1 antigen is not present on immature T cells. MT1 reacts with a membrane-bound antigen which occurs on all T cells. MT1 also reacts with thymocytes, monocytes, macrophages, epidermal Langerhans cells and the Kupffer cells of the liver, as well as with myeloid cells and erythrocyte precursors. The MT1 antigens are not found on mature or activated B cells. This mixture of monoclonal antibodies MT1 and MB1 stains membrane of all leukocytes, and recognizes all T and B cells, as well as NK cells, myeloid cells, monocytes, histiocytes and erythrocyte precursors.



Tonsil stained with Anti-CD43 & Anti-CD45RA cocktail using AEC chromogen

Clone: MT1 & MB1

Isotype: IgG1

Source: Mouse

Immunogen: Hodgkin’s lymphoma

Specificity: Leukocyte




P


CD43 (T Cell, Leukosialin)

The CD43 antigen, also known as leukosialin or sialophorin is a 95-110 kD protein. Monoclonal DFT-1 reacts with this protein on T cells and thymocytes and a 115-135 kD molecule on neutrophils and platelets. In addition, the CD43 epitope is present on many cells such as granulocytes, monocytes, macrophages, NK cells, platelets, activated B cells, plasma cells, epidermal Langerhans cells and also on bone marrow hematopoietic stem cells. This antibody stains CD43, a membrane-bound antigen found on all T cells, macrophages, monocytes, and epidermal Langerhans cells.





Clone: DFT-1

Isotype: IgG1 Kappa

Source: Mouse

Immunogen: Immature pluripotential human leukemia cell line K562

Specificity: CD43 and lymphoma or leukemia subtyping



Manual: HK087-5KE

Xmatrx: HX031-YCDE

P


CD44 (Phagocytic Glycoprotein-1, HCAM)

CD44 (phagocytic glycoprotein-1, homing cell adhesion molecule, HCAM, CD44s) is a cell surface 80-90 kD glycoprotein important in lymphocyte homing, T-cell activation and adhesion to hyaluronate and matrix proteins. It is expressed on the surface of a wide variety of cells, among which are T-cells, B-cells, monocytes, fibroblasts, keratinocytes, vascular endothelial cells, columnar epithelium of the GI tract, and transitional epithelium of the urinary tract. This antibody stains CD44 antigen in cell membranes of various cells such as T cells, B cells, monocytes, granulocytes and even on most erythrocytes, epithelial cells, central nervous white matter, fibroblasts, skeletal muscle and on a wide variety of tumors.




Breast Tissue stained with Anti-CD44 using DAB chromogen

Clone: DF1485

Isotype: IgG2b

Source: Mouse

Immunogen: Cell surface glycoprotein CD44


Pre-treatment: AR Citra

Manual: HK087-5KE

Xmatrx: None

P


CD45 (Leukocyte Common Antigen, LCA)

This antibody against CD45 (Leukocyte Common Antigen) recognizes the 200 kD antigen found on lymphoid cells, macrophages, histiocytes, and neutrophils. CD45 is helpful in determining the leukocytic nature of anaplastic tumors. Combined with other antibodies such as those to cytokeratins and S-100 protein, this monoclonal antibody to leukocyte common antigen can be used in the characterization of undifferentiated large cell neoplasms. Most neoplastic B cells and T cells stain positive in leukemia and in non-Hodgkins lymphomas, whereas most neoplastic myeloid and erythroid cells are negative. This antibody labels lymphoid cells and to a lesser extent macrophages, histiocytes, and granulocytes.



Tonsil expressing strong LCA positivity using DAB chromogen

Clone: PD7/26/16 & 2B11

Isotype: IgG1 Kappa

Source: Mouse

Immunogen: Human lymphocytes

Specificity: CD45


Pre-treatment: None

P


73

Primary Antibodies


CD45 (Leukocyte Common Antigen, LCA)

The Leukocyte Common Antigen consists of a family of heavily glycosylated glycoproteins of apparent MW 180-240kD. CD45 may function in the regulation of L-selectin (CD62L), in regulation of B-lymphocyte negative and positive selection and in T-cell activation. It stains lymphocytes, monocytes, eosinophils, and also neoplastic cells of lymphoid origin. Neoplastic B cells and T cells in leukemia and in non-Hodgkin's lymphomas stain positive. This antibody stains CD45 antigen in membrane and cytoplasm of the majority of human leukocytes.



Spleen Tissue stained with Anti-CD45 using DAB chromogen

Clone: LJ 27.9

Isotype: IgG1

Source: Mouse


Specificity: Leukocyte Common Antigen



Manual: None


P


CD45 Cocktail (Leukocyte Common Antigen, LCA)

CD45 (LCA) is a transmembrane protein-tyrosine-phosphatase. The LCA family includes a group of proteins present on all mature B and T lymphocytes, thymocytes, macrophages, spleen, lymph node, chronic lymphatic leukemia cells, bone marrow, thymus, and granulocytes. It is absent in brain, kidney, liver, heart, erythrocytes, platelets, and normal serum. This antibody may be useful in the evaluation of malignant lymphoma and nonlymphoid tumors. Neoplastic B and T cells in leukemia and in non-Hodgkin's lymphoma stain positive and hence can be distinguished from sarcomas and carcinomas. This antibody stains CD45 antigen on the membrane of most leukocytes.



FG-371MRecommended Positive Control:FB-371MRecommended Barrier Control:

LCA positivity on Anaplastic Large Cell Lymphoma stained using DAB chromogen

Clone: MEM55+LJ 27.9

Isotype: IgG1

Source: Mouse


Specificity: CD45


Pre-treatment: None

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CD45RA (B Cell)

CD45RA is a restricted isoform of LCA of about 220 kD. MB1 recognizes a membrane-bound antigen which occurs on all B cells with the exception of plasma cells, and on some mature T cells. The antigen which is identified by this monoclonal antibody is not present on immature T cells. This antibody stains CD45RA antigen on the membrane of all B cells with the exception of plasma cells and some mature T cells.




Tonsil stained with Anti-CD45R using DAB chromogen

Clone: MB1

Isotype: IgG1

Source: Mouse

Immunogen: Hodgkin’s lymphoma cell line DEV

Specificity: CD45RA


Pre-treatment: None

P

AM157-10M


AX157-YCD

CD45RB

CD45RB, which includes three glycoproteins with molecular mass of 190, 205 and 220 kD, has been found to belong to the CD45 family. Monoclonal antibody MEM55 can be used to stain CD45RB antigen on most T cells, B cells, monocytes, and macrophages. The cellular distribution of CD45RB is very similar to that of other conventional CD45 antibodies, except that some cells, such as Langerhan's cells and a small subset of T cells, are negative. This antibody stains the CD45RB antigen, the isoform of the leukocyte common antigen encoded by exon B.

Tonsil stained with Anti-CD45RB using DAB chromogen

Clone: MEM55

Isotype: IgG1

Source: Mouse


Specificity: CD45RB antigen



Manual None


P





Primary Antibodies


AM156-5M

MU156-UCFG-156M

FB-156M

AX156-YCD

CD45RC (T Cell)

Clone MT2 has previously been described as CD45RA but due to its reactivity with transfectants and its identical staining pattern with ORTH75E4 it is now recognized as CD45RC. Clone MT2 reacts with membrane-bound antigen which is present on mature, non-activated T and B cells. It reacts with medullary thymocytes, with mantle zone lymphocytes in follicles of lymph nodes and spleen, with lymphocytes of the paracortex, with peripheral blood B cells, with T suppressor/cytotoxic cells and NK cells. This clone is used for differentiation of non-Hodgkin lymphomas.


Concentrated:


T Cells in Tonsil stained with Anti-CD45RC using DAB chromogen

Clone: MT2

Isotype: IgG1

Source: Mouse

Immunogen: Lymph node involving chronic lymphatic leukemia

Specificity: CD45RC


Pre-treatment: None

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CD45RO (T Cell)

This antibody recognizes a 185 kD molecule (CD45RO) which occurs on mature activated T cells, most thymocytes, and a sub-population of resting T cells within both the CD4 and CD8 subsets. UCHL-1 shows no reactivity with normal B or NK cells, but will react with granulocytes and monocytes. This antibody can be used as a marker of T cell lymphomas and other T cell neoplasms. The antigen has been shown to be immunologically unrelated to the lymphocyte-function-associated antigen (LFA-1), which has a similar molecular weight. This antibody stains the membrane and sometimes the cytoplasm of CD45RO positive cells.


Concentrated:


AM113-5M

MU113-UC

FG-113M


Clone: UCHL-1

Isotype: IgG 2a Kappa

Source: Mouse

Immunogen: IL-2 dependent T cell line CA1

Specificity: T cells


Pre-treatment: None

Tonsil stained with Anti-CD45R0 using DAB chromogen

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CD48

CD48 (BLAST1) is an activation-associated, glycosylphosphatidylinositol (GPI)-anchored cell surface glycoprotein expressed primarily in mitogen-stimulated human lymphocytes. CD48 is expressed on T cells, B cells, thymocytes and splenocytes. Both normal and malignant white blood cells express CD48 on their membrane surface, but greater than 95% of CD34+ hematopoietic stem cells do not express CD48. CD48 is expressed at higher levels on human Burkitt’s lymphoma cell lines, Raji and most acute myeloid leukemia cells with phenotype CD34-/CD13+/CD33+.


Clone: EP148

Isotype: IgG

Source: Rabbit






Xmatrx: None


AN721-10ME


P




CD53

Leukocyte surface antigen CD53 is a protein that in humans is encoded by the CD53 gene. The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. The function of this family in a number of different cell types may be involved in transmembrane signal transduction and regulation of cell proliferation and differentiation, or both. CD53 is broadly expressed on leukocytes, including B cells, T cells, monocytes and granulocytes. It has been demonstrated to be a specific and reliable marker for leukocytes. This antibody strongly labels normal and neoplastic cells with hematopoietic origin.



Clone: EP179

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues of human CD53

Specificity: CD53


Pre-treatment: AR Citra Plus


Xmatrx: None

AN734-10M



P



75

Primary Antibodies


CD56 (Natural Killer Cell, NCAM)

The NKH-1 antigen is present on a subpopulation of 10 to 15% of human peripheral blood lymphocytes (PBL) and is expressed on all cells which mediate non-MHC restricted cytotoxicity. Therefore, it is a pan natural killer (NK) cell antigen. This antibody reacts with one of the three distinct epitopes that have been identified: the NKH1b epitope. It can also be of value in the immunophenotyping of tumors derived from neuroectodermal tissue. This antibody stains CD56 (NKH-1) on peripheral blood large granular lymphocytes in frozen tissue sections.

Clone: NKH-1

Isotype: IgG1 Kappa

Source: Mouse

Immunogen: Human chronic myeloid leukemia cells

Specificity: CD56 (NKH-1)



Manual: None

Xmatrx: HX031-YCDE/HX032-YCDEFrozen Tonsil stained with Anti-CD56 using AEC chromogen

F




CD57 (Natural Killer Cell)

Monoclonal antibody NK-1recognizes CD57, also called HNK-1 or Leu 7 antigen. It is a 110 kD myeloid, cell-associated surface glycoprotein. The antigen is common to leukocytes and neuroectodermal cells. It is present in most cancers with neuronal as well as glial characteristics. Tumors and normal cells derived from the neuroectoderm or the APUD (diffuse neuroendocrine system) tumors also express this antigen. Anti-natural killer cell antibodies used in combination with anti-S-100 antibodies aid in the differentiation of Schwann cell neoplasms from histologically similar fibrosarcomas. This antibody stains CD57 on the membrane of natural killer cells in both normal and abnormal tissues.





Clone: NK-1

Isotype: IgM

Source: Mouse

Specificity: CD57 (natural killer cell, also called HNK1)


Pre-treatment: None

P


CD61

Platelet membrane glycoprotein Integrin ß-3 (GP IIIa) forms a Ca2+-dependent heterodimer complex with GP Ilb. The GP Ilb-IIIa complex constitutes the fibrinogen and fibronectin receptor on stimulated platelets. A biochemically and immunologically similar member glycoprotein complex is present on endothelial cells. Data suggest that tyrosine phosphorylation of an integrin beta subunit may be important in initiating outside-in signaling cascades by inducing association of signaling components directly with the integrin.


AN482-10M



Kidney tissue stained with Anti-Integrin ß-3A (CD61) using AEC chromogen

Clone: EP2417Y

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues near the C-terminus (the cytoplasmic region) of human Integrin ß-3A.

Specificity: CD 61




Xmatrx: HX031-YCDE

P


CD63

CD63 is a 53 kD lysosomal membrane glycoprotein is expressed on activated platelets, monocytes and macrophages, also weakly expressed on granulocytes, T cell and B cells. It is strongly expressed in early melanoma, breast carcinoma, merkel cell carcinoma, astrocytoma and lung adenocarcinoma. Recent reports also indicate that CD63 is a good prognostic biomarker for human astrocytomas and earlier stages of lung carcinoma.

Prostate tissue stained with anti-CD63 using DAB chromogen

Clone: EP211

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues of human CD63

Specificity: CD63


Pre-treatment: EZ-AR™ 2/ EZ-AR 1


Xmatrx: HX031-YCDE


AN720-10ME



P



Primary Antibodies


CD66

Clone BY114, also known as NCA90 (Non-cross reacting antigen 90), is a unique monoclonal antibody that recognizes CD66CE which is a 90 kD antigen found principally on neutrophils. In contrast to many antibodies which recognize granulocyte-associated antigens present on other leukocytes, this antibody recognizes only granulocytes. The anti-CD66 monoclonal antibody, therefore, is very useful for differentiation of normal and neoplastic cells of granulocyte origin. Monoclonal antibody BY114 can be used to stain neutrophils in tonsil, spleen, liver, kidney, pancreas, and lung. This antibody stains phosphatidylinositol (PI) linked protein on granulocyte and squamous epithelium.



Reactive node stained with Anti-CD66 using DAB chromogen

Clone: BY114

Isotype: IgG

Source: Mouse

Immunogen: Human B cell lymphoma



Pre-treatment: None

P


CD68




CD68 antigen is a 110-kD type 1 membrane glycoprotein, appears in endosomes or lysosomes (long variant) and to a lesser extent on the cell surface (short variant). It is highly expressed by blood monocytes and tissue macrophages. It is also reported to be expressed in immature myeloid cells, lymphoma, many tumor cell lines, and some epithelial tumors, although the labeling is usually less intense than in macrophages. Clone KP1 reacts strongly with a fixative-resistant epitope of CD68 protein that is expressed by virtually all macrophages of the human body. The CD68 antibody can be used as part of a panel in the evaluation of poorly differentiated neoplasms in cytological materials.

Lymph node stained with Anti-CD68 using DAB chromogen

Clone: KP1

Isotype: IgG1 Kappa

Source: Mouse

Immunogen: Lysosomal granules from human lung macrophage

Specificity: Macrophages




Xmatrx: HX031-YCDE

P


CD68

The CD68 antigen is a heavily glycosylated transmembrane protein of 87-115 kD which is specifically expressed by tissue macrophages, Langerhans cells and at low levels by dendritic cells. This antibody is capable of staining monocytes, Kupffer cells, osteoclasts, granulocytes and their precursors; Lymphomas are negative or show a few granules. This antibody may be useful for the identification of myelomonocytic and histiocytic tumors. CD68 may help to distinguish malignant fibrous histiocytoma from other pleomorphic sarcomas. However, since CD68 detects a formalin-resistant epitope that may be associated with lysosomal granules, other lysosome-rich cells may also produce positive results.

Histocytoma stained with Anti-CD68 using DAB chromogen

Clone: CD68/G2

Isotype: IgG1

Source: Mouse


Specificity: CD68




Xmatrx: HX031-YCDE


AM549-10M



P


CD71 (Transferrin Receptor)

This antibody reacts with CD71 antigen (also known as T9 or Transferrin Receptor), a homodimeric type II membrane protein consisting of two identical subunits of approximately 95 kD covalently linked by two intermolecular disulfide bonds. This antigen has also been identified on the endothelium of brain capillaries, on carcinomas and sarcomas of various origins as well as on both high- and low-grade malignant lymphomas. This antibody stains the T9 antigen activated lymphocytes, myelocytes, and nucleated erythrocyte precursors in frozen tissue sections.


Clone: T9

Isotype: IgM Kappa

Source: Mouse

Immunogen: Human T cell chronic lymphocytic leukemia cells

Specificity: CD71 (Transferrin Receptor T9)



Manual: None


Frozen Liver stained with Anti-CD71 using DAB chromogen

F


77

Primary Antibodies


CD71 (Transferrin Receptor)

This antibody reacts with CD71 antigen (also known as T9 or Transferrin Receptor), a homodimeric type II membrane protein consisting of two identical subunits of approximately 95 kD covalently linked by two intermolecular disulfide bonds. Transferrin Receptor is present on 10% of thymocytes, activated lymphocytes, myelocytes, and nucleated erythrocyte precursors. Broad distribution of Transferrin Receptor (TR) has been observed on carcinomas and sarcomas of various origins and malignant lymphomas. Clone H68.4 is specifically directed against the human Transferrin Receptor cytoplasmic tail. This antibody stains the cytoplasm and membrane of activated lymphocytes and erythroid precursors.




Clone: H68.4

Isotype: IgG1

Source: Mouse

Immunogen: Baculovirus-expressed, recombinant human Transferrin Receptor

Specificity: CD71 (Transferrin Receptor)


Pre-treatment: AR Citra/EZ-AR 1/EZ-AR 2

Manual: HK087-5KE


P


CD74 (B Cell)

LN2 recognizes the 35 kD Class II invariant chain expressed in the nuclear membrane and cytoplasm of B lymphocytes and is suitable for differentiating between B-cell and T-cell lymphomas. It reacts with a nuclear membrane antigen expressed by B cells of mantle zones and germinal centers, and with the nuclear membrane of interdigitating cells in lymph nodes. It also reacts with Reed-Sternberg cells and their variants in Hodgkin’s disease, and sporadically with antigens expressed by tumor cells of epithelial origin. This antibody stains nucleus, membrane and cytoplasm of B-cells.





Clone: LN2

Isotype: IgG1

Source: Mouse

Immunogen: Nuclei from diffuse histiocytic lymphoma cells (SU-DHL-4)

Specificity: CD74




Manual: None

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CD79a

CD79a, also known as Ig-alpha, mb-1 membrane glycoprotein, is a type I membrane glycoprotein with a total of 226 amino acids and a molecular weight of 47 kD. CD79a forms a heterodimer with CD79b through disulfide-bonds and further forms a complex in a noncovalent fashion with membrane immunoglobulins. Both CD79a and CD79b are expressed almost exclusively on B cells and B-cell neoplasms. In addition, CD79a and CD79b antibodies are useful markers in the evaluation of precursor B-acute lymphoblastic leukemia (pre-B-ALL) because many of these tumors are negative for other B-cell markers, such as CD20 and CD45RA.




Tonsil tissue stained with Anti-CD79a using DAB chromogen

Clone: 11E3

Isotype: IgG2a

Source: Mouse

Immunogen: Recombinant CD79a protein internal domain

Specificity: CD79a antigen




Xmatrx: HX032-YCDE

P

AM414-10M Ready-to-use (Automated): i6000TM Xmatrx® AY414-YCD

CD79a

CD79 consist of two proteins, CD79a (mb-1) and CD79b (B29). CD79a recognizes the Ig-alpha protein, and CD79b recognizes the Ig-beta protein of the B-cell antigen component of the B-lymphocyte antigen receptor. CD79a is an excellent marker for identification of normal and neoplastic B lymphocytes. It has been found to be co-expressed with CD3 in 10% of cases of T-lymphoblastic leukemia/lymphoma. Antibodies to CD79α may also be useful in the differential diagnosis of Hodgkin’s disease.

Lymph node stained with anti-CD79a using DAB chromogen

Clone: EP82

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide derived from the extracellular region of human CD79a protein

Specificity: CD79a




Xmatrx: HX031-YCDE


AN719-10M



P



Primary Antibodies


CD90

CD90 is expressed on thymocytes, neurons, glial cells, endothelial cells, fibroblasts, fetal liver cells and hematopoietic stem cells in normal bone marrow and cord blood. Thy-1 has been used as a marker for a variety of stem cells and for the axonal processes of mature neurons. CD90 is associated with unfavorable clinical and biological features in acute myeloid leukemia. In prostate cancer, CD90 has been reported to be overexpressed in cancer associated fibroblasts and serves as a marker for prostate cancer-associated stroma.

Thymus stained with anti-CD90

Clone: EP56

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues near the N-terminus of human CD90 protein

Specificity: Human CD90 protein




Xmatrx: None


AN733-10M



P



CD95

The CD95 (Fas) protein is a cell surface receptor belonging to the tumor necrosis factor (TNF) family that transduces death signaling on engagement by multimeric Fas ligand (CD95L). CD95 plays a major role in determining morphological and functional maturity in a variety of systems, including the formation of the neural network and clonal deletion of autoreactive T cells. The expression of CD95 serves as a prognostic marker in predicting the outcome of disease progression and treatment in many types of tumors.


Clone: EP208

Isotype: IgG

Source: Rabbit


Specificity: CD95




Xmatrx: None


AN742-10M


P


CD99

CD99, or Protein MIC2, is a T-cell surface glycoprotein involved in the T-cell adhesion process and spontaneous rosette formation with erythrocytes. The cell surface antigen defined by the monoclonal antibody CD99 is expressed by both the human X and Y chromosomes; the gene loci on the X and Y chromosomes are referred to as MIC2X and MIC2Y, respectively. It was demonstrated that engagement of distinct epitopes on CD99 rapidly induces T cell death by a novel caspase-independent pathway. Thus, it is suggested that CD99 is linked to a novel death pathway that may have biologic relevance in control of early T cells.


AN518-10M



Ewing’s Sarcoma stained with Anti-CD99 using DAB chromogen

Clone: EPR3097Y

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues on the C- terminus of human CD99

Specificity: CD99





P


CD99 (MIC2 Antigen)

CD99 is a 32 kD membrane glycoprotein expressed by human thymocytes, most T-ALL cells, some red blood cells, and the small cell round tumors of Ewing’s sarcoma and peripheral neuroectodermal tumors. The CD99 protein is known to be involved in T-cell-adhesion events. CD99 has been found to be expressed in lymphoblastic lymphomas, large cell lymphomas, and many cases of pediatric acute lymphocytic leukemia. This antibody stains CD99 antigen in human thymocytes and some T-ALL isolates and other positive cells.


AX355-YCD Concentrated:

Ewings sarcoma stained with Anti-CD99 stained using DAB chromogen

Clone: HO36.1.1

Isotype: IgM

Source: Mouse

Immunogen: Purified E-rosette forming cells from human peripheral blood lymphocytes

Specificity: CD99 (MIC2 antigen)



Manual: HK058-5KE

Xmatrx: HX032-YCDE

P

AM355-10MReady-to-use (Automated): i6000TM Xmatrx®

MU355-UC

79

Primary Antibodies


CD103

CD103, also known as integrin alpha E (ITGAE), is an integrin protein that in humans is encoded by the ITGAE gene. CD103 is expressed on intraepithelial lymphocytes in mucosal areas, including lung and GI tract. In malignancies, CD103 is expressed on more than 95% of intraepithelial CD8+ cells and on 40% of mucosa-associated T cells, whereas less than 2% of resting blood lymphocytes are CD103-positive. In several malignant conditions, such as T-cell lymphomas and hairy cell leukemia, the cells express CD103.

Colon carcinoma stained with anti-CD103 using DAB chromogen

Clone: EP206

Isotype: IgG

Source: Rabbit


Specificity: CD103




Xmatrx: None


AN739-10ME


P




CD105

CD105 (endoglin) is a proliferation-associated and hypoxia-inducible protein abundantly expressed in angiogenic endothelial cells. It is a receptor for Transforming Growth Factor (TGF) -beta1 and -beta3 and modulates TGF-beta signaling by interacting with TGF-beta receptors I and/or II and hence driving tumor growth and metastasis. Endoglin (CD105) is a better marker to identify proliferating endothelium involved in tumor angiogenesis than pan-endothelial markers such as CD31, CD34 and Factor VIII etc. It can be used as a marker for microvessel density measurement and also in tumor imaging. This antibody stains membrane and cytoplasm of activated endothelial cells.

Uterine blood vessels stained with Anti-CD105 using DAB chromogen

Clone: 4G11

Isotype: IgG2a

Source: Mouse

Immunogen: Prokaryotic recombinant protein corresponding to a region of the external domain of the CD105 glycoprotein.

Specificity: CD105



Manual: HK058-5KE


P





CD117

c-Kit (CD117) is a transmembrane, tyrosine kinase receptor and proto-oncogene product which is expressed on numerous diverse fetal and adult cells including hematopoietic cells, mast cells, melanocytes, germ cells, and the interstitial cells of Cajal. Its expression in tumors is also diverse.




Appendix tissue stained with Anti-CD117 using DAB chromogen

Clone: T595

Isotype: IgG1 Kappa

Source: Mouse

Immunogen: Recombinant protein corresponding to the three N-terminal C2-like extracellular domains.

Specificity: c-Kit protein (CD117)




Xmatrx: HX031-YCDE

P


CD117

CD117 / c-Kit is a transmembrane tyrosine kinase encoded by the c-Kit proto oncogene. c-Kit acts to regulate a variety of biological responses including cell proliferation, apoptosis, chemotaxis and adhesion. Ligand binding to the extracellular domain leads to autophosphorylation on several tyrosine residues within the cytoplasmic domain, and activation. Mutations in c-Kit have been found to be important for tumor growth and progression in a variety of cancers including mast cell diseases, gastrointestinal stromal tumor, acute myeloid leukemia, Ewing sarcoma and lung cancer. Phosphorylation at tyrosine 721 of c-Kit allows binding and activation of PI3 kinase.


Concentrated:


AN465-5M

NU465-UC

FG-465N

Clone: YR145

Isotype: IgG

Source: Rabbit

Immunogen: CD117 antigen

Specificity: CD117

Localization: Membrane and Cytoplasm



Xmatrx: HX031-YCDE/HX032-YCDEGIST stained with Anti-CD117 using DAB chromogen

P

AN465-10M Ready-to-use (Automated): i6000TM Xmatrx® AX465-YCD

Primary Antibodies


CD146

CD146 (cluster of differentiation 146) labels endothelial cells, smooth muscle cells, intermediate trophoblast, subpopulation of T cells, and peripheral neuronal cells. In tumor, CD146 is expressed on tumor cells derived from peripheral nerves system, melanoma and clear cell sarcoma. CD146 has been used as a marker for intermediate trophoblast. It has been reported that CD146 is useful in differentiation of mesothelioma (CD146 positive) and reactive mesothelium (CD146 negative). CD146 is associated with tumor progression and the development of metastasis in human malignant melanoma.

Placenta stained with anti-CD146 using DAB chromogen

Clone: EP54

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues in human CD146 protein





Xmatrx: HX032-YCDE

P


AN716-10M





CD205

CD205 is predominantly expressed by the thymic cortical epithelium and by dendritic cells (DC), but can also be detected at low levels in T and B lymphocytes and several other epithelial cell types. CD205 is a novel thymic epithelial marker that is important for the positive selection process of thymocytes. It is a sensitive and specific marker for thymoma, while the sensitivity to thymic carcinoma is lower than CD5 and CD117.


Clone: EP176

Isotype: IgG

Source: Rabbit


Specificity: CD205




Xmatrx: HX031-YCDE


AN737-10ME


P




CD227 (Mucin 1)

Mucins are a family of high molecular weight, heavily glycosylated proteins (glycoconjugates) produced by many epithelial tissues in vertebrates. CD227, also known as mucin 1 is a breast cancer associated mucin encoded by the Muc-1 gene. CD227 is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. This protein is over expressed abundantly in >90% breast carcinomas and metastases.

Mucinous adenocarcinoma stained with Anti-CD227 using DAB chromogen

Clone: VU-4H5

Isotype: IgG1

Source: Mouse

Immunogen: 60mer tandem repeat of VTSAPDTRPAPGSTA -PPAHG,conjugated to BSA

Specificity: CD227 (MUCIN 1)


Pre-treatment: EZ-AR™ 2 /EZ-AR 1


Xmatrx: HX031-YCDE

P


AM534-10MReady-to-use (Automated): i6000TM

Xmatrx®



AX534-YCD

CDw75 (B Cell)

CDw75 is proposed as the ligand for CD22 mediating B-cell to B-cell interaction. Clone LN1 recognizes a sialoantigen on cell membranes. On tissue sections, it causes a marked reaction with the B lymphocytes in germinal centers, but only a faint reaction with B lymphocytes of the mantle zone in lymphatic tissues. LN1 also reacts with various types of epithelial cells, including cells of the distal renal tubules, breast, bronchus, prostate, and erythrocytes. This antibody stains CDw75 antigen on cell membranes of B lymphocytes in the germinal centers, certain epithelial cells, including cells of the distal renal tubules, breast, bronchus and prostate.


MU152-UC

AX152-YCDReady-to-use (Automated): Xmatrx® Concentrated:


Tonsil stained with Anti-CDw75 using AEC chromogen

Clone: LN1

Isotype: IgM

Source: Mouse

Immunogen: Nuclei from pokeweed mitogen-stimulated peripheral blood lymphocytes

Specificity: CDw75 antigen




P

81

Primary Antibodies


CDX-2

CDX2, a member of the caudal-related homeobox family, is an intestine-specific transcription factor that regulates both proliferation and differentiation in intestinal epithelial cells. It plays an important role in triggering cells towards the phenotype of differentiated villus enterocytes as well as in the maintenance of the phenotype. Clone CDX2-88 reacts with a conserved epitope of the 40kD CDX2 protein localized in the nucleus. It exclusively marks nuclei of colonic epithelial cells and colorectal cancers on formalin-fixed, paraffin-embedded tissue sections.


MU392A-UCConcentrated:


Intestine tissue stained with Anti-CDX2 using DAB chromogen

Clone: CDX2-88

Isotype: IgG 1 Kappa

Source: Mouse

Immunogen: A Balb/c mouse was immunized with a full-length CDX2 recombinant protein. Stable hybridomas were produced by fusion of spleen cells with P2/0 myeloma cell.

Specificity: CDX2 protein




Xmatrx: HX032-YCDE

P


c-erbB-2 (HER-2/neu)

Her-2/neu (c-erb-B2) gene product is a 185 kD transmembrane glycoprotein associated with tyrosine kinase activity. The antibody CB11 is directed against the internal domain of this oncoprotein. Approximately 20-30% cases of breast cancer show an amplification and/or overexpression of Her-2 in tumor cells. Since there is an availability of the target therapy to Her-2 Neu positive tumors, the lab testing of Her-2/neu in breast carcinoma becomes very important in patient care.

Breast carcinoma stained with Anti-Her2 using DAB Chromogen

Clone: CB11

Isotype: IgG1

Source: Mouse

Immunogen: Synthetic peptide corresponding to a site on the internal domain of the c-erbB-2 Protein (HER-2/neu)

Specificity: Her 2


Pre-treatment: None

Ready-to-use (Manual): AM134-5ME

MU134-UCEConcentrated:

P

AM134-10ME

FB-134MRecommended Barrier Control:FG-134MRecommended Positive Control:

Ready-to-use (Automated): i6000TM Xmatrx® AX134-YCDE

c-erbB-2 (HER-2/neu)

HER2 (human epidermal growth factor receptor 2), also known as Neu, ErbB-2, CD340 (Cluster of differentiation 340) or p185, is a protein that is encoded by the ERBB2 gene. HER2 is a member of the epidermal growth factor receptor (EGFR/ErbB) family. The kinase activity of the ErbB2 can be activated without ligand if it is overexpressed, and by association with other ErbB proteins. Overexpression of ErbB2 is detected in almost 40% of human breast cancers. For research use only. Not for use in diagnostic procedures.

Breast stained with anti-Her2 using DAB chromogen

Clone: EP3

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues surrounding tyrosine 877 of human HER2

Specificity: Her2




Xmatrx: None


AN726-10ME


P



FB-726NRecommended Barrier Control: RUO

c-erbB-3 (HER-3)

The c-erbB-3 gene product is a 180 kD transmembrane glycoprotein showing tyrosine kinase activity. It belongs to a family of growth receptors that show structural similarity to Epidermal Growth Factor Receptor (EGFR) and the c-erbB-2 proteins. The c-erbB-3 protein is widely expressed in digestive, urinary and respiratory tracts, the circulatory systems, female and male reproductive system but not in hematopoetic system. C-erbB-3 protein has also been seen to be overexpressed in some tumors including those of the breast, stomach, pancreas, colon, and ovary. This antibody stains c-erbB-3 protein in membrane of positive cells.




Breast carcinoma stained with Anti-c-erbB-3 using DAB chromogen

Clone: RTJ1/A2

Isotype: IgM

Source: Mouse

Immunogen: Synthetic peptide from the cytoplasmic domain of the human c-erbB-3 protein

Specificity: c-erbB-3 protein



Manual: HK167-5K


P


Primary Antibodies


Chromogranin A

This antibody recognizes Chromogranin A (68 kD) and other related chromogranin polypeptides from human, monkey, and pig. Chromogranin is widely distributed and through immunohistochemistry, chromogranin has been demonstrated in several elements of the diffuse neuroendocrine system, including anterior pituitary, thyroid parafollicular C cells, parathyroid chief cells, pancreatic islet cells, intestinal enteroendocrine cells, and tumors derived from these cells. The measurement of Chromogranin A has become a valuable tool in the investigation of neuroendocrine neoplasia. This antibody recognizes Chromogranin A (68 kD) and other chromogranin polypeptides in cytoplasm of positive cells.




Pancreas tissue stained with Anti-Chromogranin using DAB chromogen

Clone: LK2H10

Isotype: IgG1 Kappa

Source: Mouse

Immunogen: Tissue from human pheochromocytoma

Specificity: Secretory storage granules in endocrine cells


Pre-treatment: None

P


AM356-5M

MU356-UCFG-356M

FB-356M

Chromogranin A

This antibody recognizes Chromogranin A (68 kD) and other related chromogranin polypeptides from human, monkey, and pig. Through immunohistochemistry, chromogranin has been demonstrated in several elements of the diffuse neuroendocrine system, including anterior pituitary, thyroid parafollicular C cells, parathyroid chief cells, pancreatic islet cells, intestinal enteroendocrine cells, and tumors derived from these cells. Chromogranin immunoreactivity was also seen in thymus, spleen, lymph nodes, fetal liver, neurons, the inner segment of rods and cones, the submandibular gland, and the central nervous system.




Pancreatic islet stained with Anti-Chromogranin A using DAB chromogen

Clone: PHE-5

Isotype: IgG

Source: Mouse

Specificity: Chromogranin A




Xmatrx: HX031-YCDE

P


Claudin 1

Claudin proteins are a family of proteins associated with tight junctions. Tight junctions are specialized regions of cell to cell contact; made up of network of strands to act a molecular gasket for preventing the leakage of ions, water, etc., between cells. They are abundant in luminial epithelial sheets where they maintain epithelial cell polarity. Different tissues exhibit different claudin composition. Anti Claudin 1 plays a major role in tight junction specific obliteration of the intercellular space, though calcium independent cell adhesion activity (by similarity), and acts as a coreceptor for HCV entry into hepatic cells.





Breast Carcinoma stained with anti-claudin 1 antibody using DAB chromogen

P

AR704-10R AW704-YCD


Clone: Polyclonal

Isotype: IgG

Source: Rabbit

Immunogen: Claudin 1

Specificity: Claudin 1

Localization: Membrane/Cell junction



Xmatrx: HX031-YCDE

Claudin-5

Claudin-5 is a member of the claudin family. Mutations in Claudin-5 have been found in patients with velocardiofacial syndrome. Claudin-5 labels endothelial cells. It has been used as a marker for endothelial lesions. Claudin-5 is also found in bronchial and lung epithelial cells. In tumors, Claudin-5 expression has been found in lung adenocarcinoma and squamous carcinoma. In serous ovarian adenocarcinoma, increased Claudin-5 expression is associated with aggressive behavior.

Lung squamous carcinoma stained with anti-Claudin 5 using DAB chromogen

Clone: EP224

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues of human Claudin-5 protein

Specificity: Claudin-5 protein

Localization: Cell junction/Membrane



Xmatrx: HX032-YCDE


AN718-10M



P


FB-718N Recommended Barrier Control:

83

Primary Antibodies


c-myc Protein

Monoclonal antibody 9E10 reacts with the AEEQKLISEEDL epitope of c-myc protein. The c-myc gene product has been shown, through molecular studies, to be an essential protein for replication of cellular DNA and for the enhancement of mRNA transcription. The activated expression of one of the proto-oncogenes, c-myc, seems to accompany abnormalities in the progression of various malignancies such as lung, breast and colon carcinomas as well as melanomas. The antibody stains c-myc protein in nucleus of positive cells.

Clone: 9E10

Isotype: IgG

Source: Mouse

Immunogen: Peptide AEEQKLISEEDL

Specificity: c-myc Protein Antigen



Manual: HK058-5K

Xmatrx: HX032-YCDEBreast carcinoma stained with Anti-BCA-225 using AEC chromogen

P





Collagen III

Collagen type III is a marker for interstitial connective tissue of the extracellular matrix. Collagen type III is diffusely present throughout the interstitial connective tissues making it a better marker than cellular fibronectin, which is more closely associated with basement membrane, and presence in extracellular matrix is minimal. In highly specialized vascular beds of spleen and glomeruli where basement membrane is prominent, little collagen type III is detected, whereas fibronectin is abundant. This antibody does not react with collagens type I, II, IV, V, VI, or VII. This antibody stains positive for Collagen type III in interstitial connective tissue but not on basement membranes.




Skin tissue stained with Anti-collagen III using AEC chromogen

Clone: HWD1.1

Isotype: IgG

Source: Mouse

Immunogen: Human collagen purified by High Performance Liquid Chromatography

Specificity: Collagen type III

Localization: ECM

Pre-treatment: Pepsin

Manual: EK000-5K

Xmatrx: None

P


Collagen IV

This antibody reacts with Collagen IV and does not cross-react with other collagen types. It does not cross-react with human vitronectin, fibronectin or chondroitin sulfate A, B, or C. The positive or negative demonstration of basal lamina using immunostaining helps to distinguish some types of benign lesions from malignant tumors such as tubular carcinoma of the breast. Schwannomas and leiomyomas and their well differentiated malignant counterparts usually immunoreact in a characteristic fashion to the monoclonal antibody for type IV Collagen. The vascular nature of neoplasms such as hemangiopericytoma and epithelioid hemangio-endothelioma can be revealed by type IV collagen with more reliability than other non-specific stains. This monoclonal antibody stains human Collagen IV in basal laminae.



Skin stained with Anti-Collagen IV using AEC chromogen

Clone: COL-94

Isotype: IgG1

Source: Mouse

Immunogen: Human Collagen IV

Specificity: Type IV collagen

Localization: Basal laminae/Cyt

Pre-treatment: Pepsin, RT, 30 min./EZ-AR 1/EZ-AR 2

Manual/i6000: EK000-5K


P


Cyclin D1

Cyclins are a family of key regulatory proteins of the cell cycle. Cyclin D1 controls the transition from G1-phase to S-phase of the cell cycle. In addition to breast carcinoma, overexpression is also seen in mantle cell lymphoma, laryngeal epithelial lesions, bladder urothelial tumors, and gastric carcinoma.




Clone: Polyclonal

Source: Rabbit

Immunogen: Synthetic peptide from C-terminus of Cyclin D1

Specificity: Cyclin D1




Xmatrx: HX031-YCDEBreast cancer tissue stained with Anti-Cyclin D1 using AEC chromogen

P

A447-10RReady-to-use (Automated): i6000TM Xmatrx® AW447-YCD

Primary Antibodies


Cyclin D1

Cyclin D1 belongs to the Cyclin D family. Cyclin D1 is required for the cell cycle G1/S transition. Amplification of overexpression of cyclin D1 plays a pivotal role in the development of various human tumors including parathyroid adenoma, tumor, colon tumor, lymphoma, melanoma and prostate tumor. Cyclin D1 also associates with and regulates the activity of transcription factors, coactivators and corepressors that govern histone acetylation and chromatin remodeling proteins.


AN474-10M



Breast Carcinoma stained with anti-Cyclin D1 using DAB chromogen

Clone: EPR2241(IHC)-32

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues near C-terminus of human Cyclin D1

Specificity: Cyclin D1




Xmatrx: HX032-YCDE

P


Cyclin E2

Cyclin E2 is a member of the cyclin E family that can associate with and activate Cdk2. Expression of cyclin E2 is essential for the control of the cell cycle at the late G1 and early S phase, and this protein may regulate distinct rate-limiting pathways during this phase. Cyclin E2 specifically interacts with Cdk inhibitors of the CIP/KIP family and activates both Cdk2 and Cdk3. Expression of the viral E6 oncoprotein in normal human fibroblasts increases levels of cyclin E2, but not cyclin E1, while expression of the E7 oncoprotein upregulates both. Abnormally high levels of cyclin E expression have frequently been observed in human cancers.


AN519-10M



Glioblastoma stained with Anti-Cyclin E2 using AEC chromogen

Clone: EP454Y

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to a residue of human Cyclin E2

Specificity: Cyclin E2




Xmatrx: HX032-YCDE

P


Cytokeratin 4

Cytokeratin 4 (CK4) is a 59 kDa intermediate filament protein associated with cytokeratin 13. It is expressed in suprabasal cells of non-keratinized stratified squamous epithelium. A mutation in the CK4 gene causes white sponge nevus. A decreased expression of CK4 is associated with head and neck squamous carcinoma. It is helpful in differentiation of squamous cell carcinoma of esophagus origin from thyroid origin.

Esophagus stained with anti-CK4 using DAB chromogen

Clone: EP4

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues on the C-terminus of human CK4 protein

Specificity: CK4




Xmatrx: HX031-YCDE


AN717-10M



P



Cytokeratin 4

Cytokeratin 4 is a 59 kD cytokeratin intermediate fillament protein. It is found in non-complying squamous epithelium such as that of the superficial and intermediate epithelial cells of the esophagus, ectocervix, tongue, vagina, larynx, pharynx, epiglotis and anus, as well as the superficial cells of the cornea. Cytokeratin 4 is also expressed in the superbasal cells of urinary bladder, transitional epithelium in single cells and cell groups of sweat glands, prostatic ducts and in cylindrical, cilliated bronchial epithelial cells. For research use only. Not for use in diagnostic procedures.




Cytokeratin 4 positivity in Tonsil stained using DAB Chromogen

PClone: 6B10

Isotype: IgG1

Source: Mouse

Immunogen: Cytokeratin 4

Specificity: Cytokeratin 4


Pre-treatment: Citra plus


Xmatrx: None


RUO

AM705-10M Ready-to-use (Automated): i6000TM Xmatrx® AY705-YCD

85

Primary Antibodies


Cytokeratin 5

Cytokeratin 5 is a high molecular weight cytokeratin found on most basal stratified squamous epithelia. Recent studies show expression for cytokeratin 5 in basal-like breast carcinomas, salivary gland carcinomas, mesotheliomas and ameloblast during enamel growth.


AN484-10M


Squamous Cell carcinoma stained with Anti-Cytokeratin 5 using DAB chromogen

Clone: EPR1600Y

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues in the head region of human cytokeratin 5




Manual: HK081-5KE

Xmatrx: HX031-YCDE

P


Cytokeratin 5 & 6

Cytokeratin 5 (58 kD) is expressed in basal, intermediate and superficial cell layers of stratified epithelia as well as transitional epithelia, and in mesothelial cells and mesothelioma. Cytokeratin 6 (56 kD) is also a high molecular weight cytokeratin expressed by proliferating squamous epithelium. Types 5 and 6 of cytokeratin (CK5/6) are in high frequency in mesothelial cells, as opposed to cells of adenocarcinoma. CK5/6 has been shown to be a reliable marker for mesothelioma and squamous cell carcinoma of the lung and Bowen’s disease. It does not react with pulmonary adenocarcinoma. CK5/6 is also used as a confirmatory immunostain to recognize lymphoepitheliomas.


AN588-10M



Skin stained with Anti-Cytokeratin 5 & 6 using DAB chromogen

Clone: EPR1600Y & EPR1602Y

Isotype: IgG

Source: Rabbit

Immunogen: Synthetic peptides corresponding to residues on the N-terminus of human Cytokeratin 5 and cytokeratin 6 respectively

Specificity: Cytokeratin 5 & 6



Manual: HK08-5KE

Xmatrx: HX031-YCDE

FG-588N

P


Cytokeratin 5 + Cytokeratin 14

CK5 labels myoepithelial cells of breast and prostate basal cells. CK5 and calretinin have been useful in mesothelioma and differentiation of adenocarcinomas, especially when facing metastatic tumors of unknown origin. Cytokeratin 14 (CK14) is a 50-kDa keratin expressed in abundance in stratified epithelial, epidermal, basal, mesothelial, and myoepithelial cells in various tissues including breast and prostate. Cytokeratin 5/14-positive breast cancers are true basal phenotype confined to BRCA1 tumors. Along with p63 and CK5, the CK14 antibody has been a useful marker for cells with basal, squamous and myoepithelial differentiation.

Prostate stained with anti-CK5&14 using DAB chromogen

Clone: EP24 + EP61

Isotype: IgGSource: Rabbit

Immunogen: CK5: A synthetic peptide corresponding to residues near the C-terminus of human CK-5 protein CK14: A synthetic peptide corresponding to residues near the C-terminus of human Cytokeratin 14 protein





Xmatrx: HX032-YCDE

P


AN730-10ME




Recommended Barrier Control: FB-730N

AN500-5M

AN500-10M

NU500-UC

FG-500N

Cytokeratin 6

The cytokeratin 6 (CK6) is expressed in a heterogeneous array of epithelial tissues under normal conditions, but is better known for its strong induction in stratified epithelia that feature an enhanced cell proliferation rate or abnormal differentiation. CK6 shows a complex regulation with constitutive and inducible components in several stratified epithelia, including the oral mucosa and skin. CK6 is strongly expressed in about 75% of head and neck squamous cell carcinomas.


Concentrated:


Skin tissue stained with Anti-Cytokeratin 6 using DAB chromogen

Clone: EPR1602Y

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues on the N-terminus of human Cytokeratin 6.

Specificity: CytoKeratin 6



Manual: HK081-5KE

Xmatrx: HX031-YCDE

P


Primary Antibodies


Cytokeratin 7

Cytokeratin 7 is a 54 kD marker of simple epithelium. Antibody to Cytokeratin 7 strongly stains all cell layers of the urinary bladder transitional epithelium. However, Cytokeratin 7 is absent from gastrointestinal epithelium, hepatocytes, proximal and distal tubules of the kidney, and myoepithelium, and also cannot be detected in the stratified epithelia of the skin, tongue, esophagus, or cervix. Cytokeratin 7 recognizes specific subtypes of adenocarcinomas and can be used to differentiate between Cytokeratin 7-positive tissues such as ovarian carcinomas and transitional cell carcinomas and Cytokeratin 7-negative tissues such as carcinomas of the gastrointestinal tract and prostate cancers.





Breast Carcinoma stained with Anti-Cytokeratin7 using DAB chromogen

Clone: OV-TL12/30

Isotype: IgG1Kappa

Source: Mouse

Immunogen: Ovarian carcinoma cells



Pre-treatment: Pepsin/EZ-AR 2


Xmatrx: HX032-YCDE

P


Cytokeratin 7

Keratins are cytoplasmic intermediate filament proteins expressed by epithelial cells. Keratin 7 (CK7) is expressed in a wide range of epithelial structures in humans. Protein expression levels of CK7 appear significantly higher in chromophobe RCC compared to oncocytoma. CK7 and parvalbumin immunostains may be useful in differentiating oncocytoma from chromophobe RCC in problematic cases. Also, the pattern of immunohistochemical expression of CK7 along with CK 20 is commonly used to assess possible primary sites of metastatic carcinomas.


AN528-10M




Clone: EPR1619Y

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues near N-tertm of human CK-7



Pre-treatment: EZ-AR™ 2/ EZ-AR 1


Xmatrx: HX031-YCDE

P


Cytokeratin 7 & 8

Cytokeratins 7 and 8 are two closely related type II cytokeratins characteristic of simple epithelia. Cytokeratin 7 is less widespread than cytokeratin 8 and is expressed in sebaceous and sweat glands and some cells of the inner hair root sheath. Cytokeratin 8 is primarily found in the non squamous epithelia. Cytokeratin 7 is usually present in adenocarcinomas of lung, breast, endometrioid tumors, transitional cell carcinoma of the bladder. The combination of cytokeratin 7 and 8 is a useful marker for differentiating adenocarcinomas and ductal carcinomas from squamous cell carcinomas.


AM587-10M



Breast carcinoma stained with Anti-Cytokeratin 7&8 using DAB chromogen

Clone: OV-TL12/30 & C51

Isotype: IgG1

Source: Mouse

Immunogen: Ovarian carcinoma cells & MCF-7 cells





Xmatrx: HX031-YCDE

P


Cytokeratin 8

Cytokeratin 8 (52 kD) and 18 (45 kD) comprise a Cytokeratin pair as markers for simple epithelia. In most situations, Cytokeratin 8 exists in tissues together with Cytokeratin 18, but there are exceptions among some normal and abnormal epithelial cells. Therefore, it is useful to use both Cytokeratin 8 and Cytokeratin 18 in combination with other anti Cytokeratin antibody monoclonals when studying cytokeratin expression patterns. Clone C-51 is designed for the specific localization of Cytokeratin 8 and does not cross-react with human cytokeratin numbers 7, 17, 18, or 19. This antibody stains Cytokeratin 8 in cytoplasm of positive epithelial cells.





Clone: C51

Isotype: IgG1

Source: Mouse

Immunogen: A cytoskeletal preparation of MCF-7 cells



Pre-treatment: AR Glyca/EZ-AR 1


Xmatrx: HX031-YCDE

P


87

Primary Antibodies


Cytokeratins 8 & 18

Carcinomas may be classified precisely by the analysis of their keratin patterns. Clone 5D3 recognizes an epitope restricted to a few members of the cytokeratin subclasses, specifically Cytokeratins 8 and 18. This antibody reacts with all simple epithelia including glandular epithelium and ciliated pseudostratified columnar epithelium localized in thyroid, female breast, gastrointestinal and respiratory tract. 5D3 may be a useful marker for demonstrating columnar cell differentiation when studying biphasic differentiation of basal cells of respiratory or intermediate epithelium.





Colon carcinoma stained with Anti-Cytokeratin 8 &18 using DAB chromogen

Clone: 5D3

Isotype: IgG1

Source: Mouse

Immunogen: Cytokeratins from human breast carcinoma cell line MCF-7

Specificity: Cytokeratins 8 and 18


Pre-treatment: Pepsin, 37°C, 5 min /EZ-AR 1


Xmatrx: HX031-YCDE

P


Cytokeratin 10

Cytokeratins 1 and 10 are expressed only in suprabasal layers, and their expression increases with epidermal maturation. In terminally differentiated keratinocytes of the stratum corneum, Cytokeratins 1 and 10 are regarded as markers for orthokeratinization. Keratinizing areas expressing Cytokeratin 10 have been demonstrated in various well differentiated squamous cell carcinomas derived from epidermis as well as from various internal sites of stratified epithelia. This antibody stains cytoplasm in epithelial cells of the stratum corneum.


Concentrated:


Skin stained with Anti-Cytokeratin 10 using DAB chromogen

Clone: DEK-10

Isotype: IgG1

Source: Mouse

Immunogen: Human epidermal keratin isolated by high salt extraction




Xmatrx: HX031-YCDE

Manual/i6000: None

MU201-UC

FG-201M

AM201-5M

P


Cytokeratin 13

Cytokeratins 4/13 are markers for stratified squamous epithelia in internal organs including esophagus and tongue. This antibody is a reliable marker for squamous metaplasia found in respiratory tissue and prostate gland. Squamous metaplasia in the respiratory tract and in some other human organs may be associated with a precancerous condition. This 51 kD Cytokeratin 13, which is expressed in internal non-keratinized stratified squamous epithelia, and its frequently co-expressed partner, the basic 59 kD Cytokeratin 4, may be regarded as markers for esophageal-type differentiation. This antibody stains most cytoplasm in stratified squamous epithelium (except skin epidermis).



Suprabasal cells in esophagus stained with Anti-Cytokeratin 13 using DAB chromogen

Clone: AE8

Isotype: IgG

Source: Mouse

Immunogen: Human epidermal keratin




Xmatrx: HX031-YCDE

Manual/i6000: None

P


Cytokeratin 14

Cytokeratin 14 (molecular weight 50 kD), an acidic (Type I) cytokeratin protein, is one of the cytokeratin pairs (50/ 58 kD) that distinguishes stratified epithelial cell types from simple epithelial types. Cytokeratin 14 is homogeneously expressed in all cells of the keratinizing squamous epithelium and is confined to the basal and parabasal cells in the nonkeratinizing squamous epithelium of the normal adult urinary tract. The monoclonal antibody to Cytokeratin 14 may be helpful in distinguishing the cell types of the human mammary gland, thus it may also be used to study histogenesis of breast carcinoma. This antibody stains Cytokeratin 14 in cytoplasm of epithelial cells.



FG-146MRecommended Posiitve Control:

Squamous cell carcinoma tissue stained with Anti-Cytokeratin 14 using AEC chromogen

Clone: LL002

Isotype: IgG1 Kappa

Source: Mouse

Immunogen: Thyroglobulin conjugated synthetic peptide representing the C-terminal (last 15 residues) of human cytokeratin 14





Xmatrx: HX031-YCDE

P


Primary Antibodies


Cytokeratin 15

Cytokeratin 15 is subdivided into acidic type I intermediate filament proteins. Cytokeratin 15 has been reported in basal keratinocytes of the epidermis and has also been proposed as marker of stem cells in the hair follicle bulge. Cytokeratin 15 can be used to differentiate limbal phenotype from the conjunctiva.


Concentrated:

AN485-5M

AN485-10M

NU485-UC

Squamous cell carcinoma stained with Anti-cytokeratin 15 using AEC chromogen

Clone: EPR1614Y

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues on the C-terminus of human CK-15





Xmatrx: HX031-YCDE

P


Cytokeratin 16

Cytokeratin 16 is a type I keratin (42 KD), observed in psoriatic epidermis, also in normal heel epidermis, trunk epidermis induced hyperproliferate by trauma. It is associated with proliferation.



Squamous cell carcinoma stained with Anti-cytokeratin 16 using AEC chromogen

Clone: EP1615Y

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues on the N-terminus of human CK-16





Xmatrx: HX031-YCDE

P

AN486-10MReady-to-use (Automated): i6000TM Xmatrx® AX486-YCD

Cytokeratin 17

Cytokeratin 17 is 46 kD intermediate filament found in simple epithelia sometimes in association with Cytokeratin 7. This antibody has been used to distinguish cervical immature squamous metaplasia from high grade cervical intraepithelial neoplasia (CIN III). Anti-CK17 also labels myoepithelial cells in the benign breast tissue. CK17 labelling of breast carcinoma cells (so-called basal phenotype) has been associated with a poor prognosis.



Squamous Cell carcinoma stained with Anti-CK17 using DAB chromogen

Clone: E27

Isotype: IgG

Source: Mouse

Immunogen: Recombinant human cytokeratin 17





Xmatrx: HX031-YCDE


P


Cytokeratin 18

Cytokeratins 8 (52 kD) and 18 (45 kD) comprise a cytokeratin pair as markers for simple epithelia. The monoclonal antibodies specific for cytokeratin 18 stain all carcinomas derived from simple epithelia but do not stain well-differentiated squamous cell carcinoma. It is useful to use monoclonal antibodies to Cytokeratins 8 and 18 in combination with other anti-cytokeratin monoclonal antibodies when studying cytokeratin expression patterns. This antibody stains Cytokeratin 18 in cytoplasm of epithelial cells.

Ready-to-use(Manual): AM143-5M



Adenocarcinoma stained with Anti-CK18 using AEC chromogen

Clone: DC-10

Isotype: IgG1

Source: Mouse

Immunogen: A cytoskeletal preparation of HeLa cells



Pre-treatment: AR Glyca/EZ-AR 1

Manual: HK167-5K

Xmatrx: HX031-YCDE

P


89

Primary Antibodies


Cytokeratin 19

Cytokeratin 19 (molecular mass 40 kD) is a marker of simple epithelia. Cytokeratin 19 has been found in mesothelial and mesothelioma cells, ovarian cysts, cystadenomas, and ovarian carcinomas, in adenocarcinomas of the lung and in tumor cells of pulmonary metastases, in the ductal cells of normal pancreas and in pancreatic cancers. It has been shown to be present in the basal layer of non-keratinizing stratified squamous epithelia such as the oral cavity and the ectocervix.




Colon carcinoma stained with Anti-Cytokeratin 19 using DAB chromogen

Clone: RCK108

Isotype: IgG1 Kappa

Source: Mouse

Immunogen: Total cell extract from human bladder cancer cell line



Pre-treatment: Pepsin, 37°C, 5 min./EZ-AR 2

Manual: EK000-5K

Xmatrx: HX032-YCDE

P


Cytokeratin 20

Cytokeratin 20 (46kD) is relatively less acidic than other type I keratins. This antibody reacts with certain types of carcinomas such as adeno carcinomas of the colon, transitional cell carcinomas of the bladder and Merkel cell tumors of the skin. It does not stain breast, lung and endometrial adenocarcinomas. The differential staining pattern of this antibody makes it very useful for tumor evaluation when used in conjunction with cytokeratin 7 staining.

Cytokeratin 20 positivity in colon carcinoma stained using DAB chromogen

Clone: IT-Ks20.8

Isotype: IgG 2a

Source: Mouse

Immunogen: Elecrophoretically purified cytokeratin 20 from human intestinal mucosa



Pre-treatment: Protease/EZ-AR 1


Xmatrx: HX031-YCDE



AM315-5M

AM315-10MAX315-YCD

MU315-UCFG-315M


P

Cytokeratin 20

Keratins are intermediate filaments expressed by epithelial cells that are involved in cell movement, differentiation and mitosis. Cytokeratin 20 (CK20) is primarily found in the goblet cells and enterocytes of gastric and intestinal mucosa. CK20 is expressed in colorectal, pancreatic, bile duct adenocarcinomas, and transitional cell carcinoma of the bladder. When used in a panel with CK7, CK20 can be particularly useful in identifying colorectal adenocarcinomas and differentiating it from other metastatic carcinomas and also identifying metastatic colorectal adenocarcinomas




Colon carcinoma stained with Anti-Cytokeratin20 using DAB chromogen

Clone: EPR1622Y

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues on the C-terminus of human Cytokeratin 20




Manual: HK081-5KE

Xmatrx: HX031-YCDE

FG-557N

P

AN557-10MReady-to-use (Automated): i6000TM Xmatrx® AX557-YCD

Cytokeratin Cocktail

Human cytokeratins (40 kD to 68 kD) are a family of water-insoluble proteins that form a major part of the cytoskeleton of epithelial cells. Immunohistochemical analysis of a large variety of neoplasms has established keratin protein immunohistochemistry as an important aid for classification of epithelial neoplasms. Monoclonal antibodies AE1 and AE3 recognize the acidic and basic subfamilies of cytokeratin, respectively. Thus, the combination of these two antibodies can be used to detect almost all human epithelia. These antibodies show no cross-reactivities with other cytoskeletal proteins. This monoclonal antibody cocktail can be used to detect almost all human epithelia. Membrane and cytoplasmic staining is seen in epithelial cells.





Skin tissue stained with Cytokeratin cocktail AE1 & AE3 using AEC chromogen

Clone: AE1 and AE3

Isotype: IgG1

Source: Mouse


Specificity: Cytokeratin


Pre-treatment: Trypsin, 37°C, 20 min/EZ-AR 1

Manual/i6000TM: EK001-5K

Xmatrx®: HX031-YCDE

P

AM071-10MAX071-YCD


Primary Antibodies


Cytokeratin Cocktail, Broad Spectrum

Human cytokeratins, which form a major part of the cytoskeleton of epithelial cells, belong to a family of water soluble proteins ranging in size from 40 to 68 kD. Various subsets of cytokeratin proteins occur in any given epithelium, depending on the epithelium cell type, stage of differentiation and embryonic development, cellular growth environment, and type of malignancy. Immunohistochemical analysis of a large variety of neoplasms has established that cytokeratin protein immunohistochemistry is an important aid for epithelial tumor classification. This monoclonal antibody stains keratin in the cytoplasm of positive epithelial cells.



Normal stomach mucosa showing cytoplasmic positivity for Cytokeratin cocktail using DAB chromogen

Clone: 34βE12/C51/AE1

Isotype: IgG1

Source: Mouse

Immunogen: Human epidermal keratin and cytoskeletal preparation of MCF-7 cells




Manual: EK000-5K

Xmatrx®: HX032-YCDE

P


Cytokeratin Cocktail, Broad Spectrum

Human cytokeratins, a family of water-insoluble polypeptides, form the major part of the cytoskeleton in all normal and neoplastic epithelial cells. CK88 is a broad spectrum antibody cocktail that reacts with a variety of normal and neoplastic epithelia. It recognizes most epithelium including simple, basal, suprabasal layers, cornea, cornifying stratified epithelium of skin, transitional epithelium of urinary tract, and squamous epithelium. Analysis of intracellular keratin by immunoperoxidase technique is helpful in establishing the epithelial nature of primary or metastatic poorly differentiated neoplasms. This antibody stains cytokeratin in cytoplasm of normal and neoplastic epithelial cells.


AM372-10M




Breast Carcinoma stained with Anti-CK88 using DAB chromogen

Clone: LL002+DEK-10+RCK108+OV-TL12/30+C11

Isotype: IgG Cocktail

Source: Mouse




Pre-treatment: Pepsin, RT, 10 min.

Manual/i6000: EK000-5K/EZ-AR 2

Xmatrx: HX032-YCDE

P


Cytokeratin, High MW

Monoclonal antibody 34βE12 is specific for "high molecular weight" cytokeratins 1, 5, 10, 14, corresponding to molecular weights of 68, 58, 56.5, and 50 kD, respectively, which are characteristically found in complex epithelium. The antibody reacts with all squamous and ductal epithelium and stains carcinomas. It reacts with benign small-acinar lesions of the prostate. This antibody stains positive in cytoplasm of epithelial cells.




Prostatic basal cells stained with Anti-Cytokeratin (HMW) using DAB chromogen

Clone: 34βE12

Isotype: IgG1 Kappa

Source: Mouse

Immunogen: Human stratum corneum

Specificity: High molecular weight cytokeratin


Pre-treatment: Pepsin, 37°C, 20 min/EZ-AR 1


Xmatrx: HX031-YCDE

P


Cytokeratin, High MW (Basic)

The cytokeratins are a family of water insoluble proteins (40-70 kD) found in almost all epithelial cell types. Monoclonal cytokeratin antibody AE3 recognizes all basic (Type II) keratins. Since each epithelium contains at least one acidic and one basic keratin, AE3 may be used as a broadly reactive antibody which stains positive for most epithelia and their neoplasms. AE3 has shown great sensitivity and broad specificity for keratins under various conditions of fixation and staining. This antibody stains positive for cytoplasm of most epithelia and their neoplasms.


FG-133M


AM133-5M


Squamous Cell carcinoma stained with Anti-Cytokeratin using DAB chromogen

Clone: AE3

Isotype: IgG

Source: Mouse

Immunogen: Total keratin was isolated from human epidermal callus. After heating to 65° C for 10 minutes, the denatured keratins were used as the antigen

Specificity: Cytokeratin high MW (basic)


Pre-treatment: Trypsin, 370C, 20 min./EZ-AR 1

Manual: EK001-5K

Xmatrx HX031-YCDE

P


91

Primary Antibodies


Cytokeratin, Low MW

The cytokeratins are a family of water insoluble proteins (40-70 kD) found in almost all epithelial cell types. Low molecular weight cytokeratin antibody AE1 has proven to be a widespread histological marker for the restricted staining of the epidermal basal layer of skin and almost all epithelially derived tumors. It can be used as a marker for cells of epithelial origin. This antibody recognizes most type I keratins and shows broad species specificity reacting with keratins of many species including human, rabbit, mouse, bovine, and chick. Staining is usually stronger in alcohol-fixed tissues than in formalin-fixed tissues.




Breast Carcinoma stained with Anti-Cytokeratin using DAB chromogen

Clone: AE1

Isotype: IgG1

Source: Mouse


Specificity: 40, 48, 50 and 56.5 kD keratins


Pre-treatment: Pepsin, RT, 10 min./EZ-AR 2


Xmatrx: HX032-YCDE

P



Cytokeratin, Pan

The Lu-5 antibody recognizes an epitope on the surface of cytokeratin filaments which is present in a wide range of cytokeratins, except in intermediate-size filament proteins. This epitope may be found in all human epithelia and carcinomas and is resistant to formalin-fixation. The Lu-5 antibody was determined is a useful pan cytokeratin marker for the detection of both normal and malignant epithelial and mesothelial cells. The Lu-5 antibody stains surface of cytokeratin filaments in a wide variety of normal and tumor tissues.


Concentrated:


Adenocarcinoma stained with Anti-Cytokeratin Pan using DAB chromogen

Clone: Lu-5

Isotype: IgG1 Kappa

Source: Mouse

Immunogen: Cells from a lung cancer cell line

Specificity: Cytokeratins



Manual: EK000-5K

Xmatrx: HX031-YCDE

FG-181M

MU181-UC

AM181-5M

P


Cytokeratin, Pan

Human keratins are a family of water-insoluble proteins with molecular weights ranging from 40-68kD. This monoclonal cytokeratin antibody can be used to detect cytokeratins 4, 5, 6, 8, 10, 13, and 18 in simple or stratified epithelium in most vertebrates including humans. It can be used as a marker for carcinomas as well as some special types of tumors which have an epithelial component or differentiation. This antibody stains cytokeratin in cytoplasm of normal and malignant epithelial cells in formalin-fixed, paraffin-embedded tissue sections, frozen sections or methanol-acetone-fixed culture cells.



Cytokeratin Pan on skin tissue stained using AEC chromogen

Clone: C11

Isotype: IgG1

Source: Mouse

Immunogen: Cytoskeletal proteins from A431 cells

Specificity: Cytokeratins


Pre-treatment: Protease, RT, 10 min./EZ-AR 1

Manual: EK002-5K

Xmatrx: HX031-YCDE

P


Cytomegalovirus (CMV)

Cytomegalovirus (CMV) is a member of the family Herpesviridae. It is found in several body fluids including saliva, urine, breast milk, cervical secretions, blood, and semen. This antibody reacts with an early non-structural antigen of 68 kD found in the nucleus of infected cells. This antibody stains CMV particles in infected tissues and does not cross-react with the Herpes viruses. For research use only. Not for use in diagnostic procedures.



Colon tissue stained with anti-CMV using DAB chromogen

Clone: BM204

Isotype: IgG1

Source: Mouse

Specificity: Cytomegalovirus


Pre-treatment: None

P

RUO

Primary Antibodies


Desmin

Desmin is a 56 kD intermediate filament expressed by cells of smooth, skeletal, and cardiac muscle. In myofibrils, desmin is localized in skeletal and cardiac muscle Z lines, in regions of cell-cell juncture, at the site of apposition of the Z line with the plasma membrane, and in cardiac intercalated disks. The specificity of desmin to muscle cells makes it a useful marker in identifying sarcomas derived from smooth and striated muscle cells such as leiomyosarcomas and rhabdomyosarcomas. This antibody does not cross-react detectably with GFAP, keratin, vimentin, or neurofilament. This antibody stains positive in muscle cells.





Skeletal muscle fibre stained with Anti-Desmin using DAB chromogen

Clone: D33

Isotype: IgG1 Kappa

Source: Mouse

Immunogen: Purified desmin from human leiomyoma

Specificity: Desmin


Pre-treatment: None

P


DOG1

DOG1 is a cell surface protein selectively expressed in gastrointestinal stromal tumors (GIST). The DOG1 protein shows no homology at the DNA or amino acid level with KIT. DOG1 antibody labels the epithelium of the following organs: breast, prostate, salivary gland, liver, stomach, testis, pancreas, and gallbladder. DOG1 is a useful marker for GISTs, including PDGFRA mutants that fail to express KIT antigen

GIST stained with Anti-DOG1 using DAB chromogen

Clone: 1.1

Isotype: IgG

Source: Mouse

Immunogen: A synthetic peptide corresponding to residues in human MUCDOG1 .

Specificity: DOG1




Xmatrx: HX031-YCDE


AM570-10M



P


Dystrophin

Dystrophin is the protein product of the Duchenne and Becker muscular dystrophy (DMD/BMD) gene with a relative molecular mass of 400 kD. This monoclonal antibody reacts with an epitope spanning the mid-rod domain between amino acids 1181 and 1388 of human dystrophin. It stains skeletal, cardiac, and smooth muscle dystrophin from normal human membrane in tissue and some animals.



Skeletal muscle stained with Anti-Dystrophin using DAB chromogen

Clone: Dys1 (Dy4/6D3)

Isotype: IgG2a

Source: Mouse

Immunogen: Bacterial fusion protein containing mid-rod domain of human dystrophin

Specificity: Dystrophin



Manual: HK058-5K

Xmatrx: HX031-YCDE

P


Dystrophin

Dystrophin is the protein product of the Duchenne and Becker muscular dystrophy (DMD/BMD) gene with a relative molecular mass of 400 kD. Antibodies to dystrophin show that DMD individuals lack dystrophin in their muscle cells or that dystrophin is present at very low levels, whereas BMD individuals produce a protein with reduced abundance or abnormal size. This monoclonal antibody reacts with an epitope spanning the mid-rod domain between amino acids 1181 and 1388 of human dystrophin. This antibody stains membrane in skeletal, cardiac, and smooth muscle dystrophin from normal human tissue and some animals.


FG-244MRecommended Barrier Control:

Skeletal muscle stained with Anti-Dystrophin using AEC chromogen

Clone: Dys2 (Dy8/6C5)

Isotype: IgG1

Source: Mouse

Immunogen: Synthetic polypeptide consisting of the last 17 amino acids at the carboxy terminus of the human dystrophin sequence

Specificity: Dystrophin



Manual: HK058-5K

Xmatrx: HX031-YCDE

P


93

Primary Antibodies


E-Cadherin

E-Cadherin (123-kD) is a cell surface glycoprotein responsible for Ca2+-dependent intercellular adhesion between epithelial cells. Alterations in the cell-cell adhesion mechanism mediated by E-Cadherin which is lightly associated with alpha catenin may have implications in the metastatic potential of prostate cancer. E-Cadherin may also play a role in adhesion of dendritic epidermal T cells to keratinocytes. Clone 36 may be used to investigate the process of tumor invasion.



Membranous positivity of E-Cadherin on Colon carcinoma stained using DAB chromogen

Clone: 36

Isotype: IgG1

Source: Mouse

Immunogen: C-terminal peptide of human E-cadherin

Specificity: E-Cadherin



Manual/i6000TM:

Xmatrx: HX031-YCDE

P

AM390-10MAX390-YCD


HK058-5K

E-Cadherin

E-Cadherin is a transmembrane glycoprotein that plays an important role in epithelial cell adhesion. In prostate cancers, the expression of E-cadherin is reported to be reduced or absent in comparison with its expression in normal prostate which is uniformly strong. A decreased expression of E-Cadherin is associated with metastatic potential and poor prognosis in breast cancer and esophagus cancer. In combination with p120 Catenin or Cytokeratin, it is useful for the differentiation between ductal (E-Cadherin positive) and lobular (E-Cadherin negative) breast carcinomas. It may also help in diagnosis of mesothelioma.

Breast carcinoma stained with anti-E-cadherin using DAB chromogen

Clone: EP6

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues in the 5th cadherin domain of human E-Cadherin protein.

Specificity: E-Cadherin




Xmatrx: HX032-YCDE


AN725-10M



P



EGFR

The rabbit antibody to EGFR (LRVAP) reacts with the 170 kD EGFR transmembrane glycoprotein. It binds specifically to the intracellular portion, regardless of phosphorylation state. The extracellular domain binds epidermal growth factor (EGF) as a proliferation signal. The EGFR antibody is made against a sequence which is unique from related tyrosine kinase receptors and hence shows no cross-reactivity.

Ready-to-use (Manual): AR335-5RE

Squamous Cell carcinoma stained with Anti-EGFR using DAB chromogen

Clone: Polyclonal

Source: Rabbit

Immunogen: Synthetic peptide encompassing amino 1195 through 1210 of human Epidermal Growth Factor Receptor

Specificity: Epidermal Growth Factor Receptor


Pre-treatment: EZ-AR1/EZ-AR2


Concentrated: PU335-UPE

P

Ready-to-use (Automated): Xmatrx® AW335-YCDE

EGFR (pan)

Epidermal growth factor receptor (EGFR) is a 170-kD transmembrane glycoprotein receptor found on a variety of cell types. After a ligand binds to a single-chain EGFR, the receptor forms a dimer that signals within the cell by activating receptor autophosphorylation through tyrosine kinase activity. Autophosphorylation triggers a series of intracellular pathways that may result in ion transport, morphological changes and proliferative activity.

Squamous Cell carcinoma stained with Anti-EGF Receptor using DAB chromogen

Clone: EP38Y

Isotype: IgG1

Source: Rabbit

Immunogen: A synthetic phospho-peptide corresponding to residues surrounding Tyr1068 of human EGF receptor.

Specificity: Human EGFR


Pre-treatment: EZ-AR1/EZ-AR2


P




AN473-10ME

Primary Antibodies


Ep-CAM

Epithelial cell adhesion molecule (Ep-CAM) is a monomeric membrane glycoprotein that is expressed on virtually all epithelial cell membranes and on a variety of epithelial carcinomas. Ep-CAM is overexpressed in a variety of human carcinomas and is, therefore, a potential therapeutic target for human solid tumors. Ep-CAM contains an extracellular domain with two epidermal growth factor-like repeats, followed by a cysteine-poor region, which are necessary for the adhesion properties of the molecule.


AN489-10M

AX489-YCDConcentrated:

Adenoma tissue stained with Anti-Epcam using DAB chromogen

Clone: E144

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues in C-terminus (cytoplasmic domain) of human Ep-CAM.

Specificity: Epcam(C-term)


Pretreatment: AR-Citra /EZ-AR 1/EZ-AR 2



P


NU489-UC


Epithelial Membrane Antigen (EMA)

Epithelial Membrane Antigen (EMA), also known as milk fat globule membrane protein, is present on the luminal surface of mammary gland epithelium. Although EMA is primarily located in mammary gland epithelium, other normal epithelia (e.g., lung) will also react against EMA antibody. Cells obtained from solid metastases and pleural effusions accompanying a breast cancer will react with EMA antibody. It may also be useful for identification of meningioma. Human colon carcinoma, osteosarcoma, kidney carcinoma, hepatocellular carcinomas, adrenal carcinoma, embryonal carcinoma, liposarcoma, lung carcinoma, and mixed parotid tumor do not stain with EMA antibody.


AM057-10M



Pancreatic tissue showing positivity for EMA stained using DAB chromogen

Clone: E29

Isotype: IgG2a Kappa

Source: Mouse

Immunogen: Delipidated extract of human cream

Specificity: EMA



Xmatrx: HX031-YCD/HX032-YCD

Manual/i6000: None

P



The mucin antigen recognized by Mc5 is a glycosylated molecule with a molecular mass of 400 kD. The sequence to which this antibody binds is Thr-Arg-Pro-Ala-Pro. Although EMA is primarily located in mammary gland epithelium, other normal epithelia (e.g., lung) will also react against EMA antibody. Staining, however, is the strongest in mammary epithelia. The combination of positive staining for keratin with negative EMA can be used to phenotype the above-mentioned epithelial tumors.


AM182-10MAX182-YCD



Breast Carcinoma stained with Anti-EMA using DAB chromogen

Clone: Mc5

Isotype: IgG1

Source: Mouse

Immunogen: Delipidated human milk fat globules

Specificity: Epithelial membrane antigen



Xmatrx: HX031-YCDE

Manual/i6000: None

P


Epithelial Specific Antigen

Monoclonal antibody MOC-31 recognizes the cluster 2 antigen which is a 40 kD transmembrane glycoprotein present on the membrane of epithelial cells. Since MOC-31 reacts with virtually all normal epithelia and adenocarcinomas but not with mesothelial cells, this antibody can serve as a reliable marker for determining the origin of pleural and peritoneal tumors. This antibody stains a membrane glycoprotein on epithelial cells, but not mesothelial cells.


AM316-10M



Adenocarcinoma stained with Anti-ESA using AEC chromogen

P


Clone: MOC-31

Isotype: IgG1

Source: Mouse

Immunogen: Cell line from small cell lung carcinoma, CD2 epithelial antigen

Specificity: 40 kD epithelial-specific cluster 2 antigen


Pre-treatment: AR Glyca/Pepsin


Xmatrx EK000-10K

95

Primary Antibodies


Epstein-Barr Virus (EBV) Early Antigen

This Epstein-Barr Virus is the causative agent of infectious mononucleosis and is associated with two human neoplasms, Burkitt’s lymphoma and nasopharyngeal carcinoma. Monoclonal antibody 1108-1 detects the diffuse early antigen of EBV. It specifically recognizes a 55;50 kD protein complex associated with the early antigen of Epstein-Barr Virus (EBV). It is recommended that this antibody be used with alkaline phosphatase label only.

Ready-to-use (Manual):Concentrated:

Cell Culture Slide stained with Anti-EBV using AEC chromogen

Clone: 1108-1

Isotype: IgG1

Source: Mouse

Immunogen: Immunoprecipitated EBV early antigens

Specificity: Immunoprecipitated EBV early antigens

Localization: Nucleus/Cytoplasm

Pre-treatment: None


AM222-5ME

MU222-UCE

FG-222M

F

erbB2 (HER-2/neu)

ErbB-2/Her-2 is one of the four members of the ErbB receptor family of transmembrane receptor-like tyrosine kinases. The kinase activity of ErbB2 can be activated without ligand if it is over expressed, and by association with other ErbB proteins. Over expression of ErbB2 is detected in almost 40% of human breast cancers. Binding of c-Cbl ubiquitin ligase to Tyr1112 of ErbB2 leads to poly-ubiquitination of ErbB2 and enhances its degradation. ErbB2 is one of the major targets for the treatment of breast cancer and other carcinomas.


Breast carcinoma stained with Anti-HER-2/neu using DAB chromogen

Clone: EP1045Y

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues near the C-terminus of human HER-2

Specificity: HER-2


P

AN471-10MEReady-to-use (Automated): i6000TM Xmatrx® AX471-YCDE


Estradiol

Estradiol plays an important role in the genesis and development of human breast cancer and endometrial carcinoma. It is synthesized primarily in the ovary, but also in the placenta, testis, and possibly the adrenal cortex. Estradiol is also produced by testicular Leydig tumors, as well as by Sertoli tumors of the testis and ovary. It is also produced in mammary gland carcinoma, and carcinoma of the adrenal cortex.



Breast carcinoma stained with Anti-Estradiol using AEC chromogen

Clone: Polyclonal

Source: Rabbit

Immunogen: 17-beta-estradiol conjugated to bovine serum albumin.

Specificity: Estradiol



Manual/i6000: None

Xmatrx: HX031-YCDE

P


Estrogen Receptor

Ready-to-use (Manual, 3 mL): AM272-2ME


The determination of steroid hormone receptors has become widely used in the management of hormone-dependent cancers. The Estrogen Receptor content of breast carcinoma is a tool for both prognosis and response to endocrine therapy. Individuals whose tumors contain estrogen receptor are more likely to respond to endocrine manipulation than those whose tumors are estrogen receptor poor. Estrogen Receptor (ER) modulates cellular events by binding estrogen response elements (ERE) when bound to estrogen in dimers. This results in the transcription of a number of proteins, including ER. Protein synthesis occurs in the cytoplasm, after which ER associates with chaperone proteins and is translocated to the nuclear membrane. The ER protein is transported into the nucleus through utilization of ATP.

Breast carcinoma stained with Anti-ER using DAB chromogen

Clone: 1D5

Isotype: IgG1

Source: Mouse

Immunogen: Recombinant estrogen receptor protein

Specificity: Estrogen receptor protein




Xmatrx: HX032-YCDE

P

AM272-10ME




Primary Antibodies


Estrogen Receptor-Alpha (ER Alpha) is a nuclear protein and member of the steroid hormone receptor family. ER Alpha possess both DNA binding and ligand binding domains, and exerts a significant role in activating the transcription of certain genes. Ligand-dependent dimerization and phosphorylation both function to regulate the transcriptional activation of ER alpha. This antibody stains nucleus of neoplastic cells in the breast ductal carcinoma tissues by immunohistochemical techniques.


Concentrated:

Estrogen Receptor

Breast carcinoma stained with Anti-ER-Alpha using DAB chromogen

Clone: EP1

Isotype: IgG

Source: Rabbit

Immunogen: Recombinant Estrogen Receptor protein





Xmatrx: None

AN710-5ME

NU710-UCE

P

AN710-10ME




ER Alpha

Estrogen receptor alpha (ER alpha) is a nuclear protein and member of the steroid hormone receptor family. ER alpha possesses both DNA binding and ligand binding domains, and exerts a significant role in activating the transcription of certain genes. Ligand-dependent dimerization and phosphorylation both function to regulate the transcriptional activation of ER alpha . The ER is an important regulator of growth and differentiation in the mammary gland. Presence of ER in breast tumors indicates an increased likelihood of response to anti-estrogen (e.g. tamoxifen) therapy.



Breast carcinoma stained with Anti-ER-Alpha using AEC chromogen

Clone: EPR703(2)

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues on the C- terminus of human ER alpha

Specificity: ER Alpha



Manual/i6000: None

Xmatrx: HX032-YCDE

P



Estrogen receptor (ER) content of breast cancer tissue is an important parameter in the prediction of prognosis and response to endocrine therapy. Highly specific monoclonal antibodies to ER have allowed the determination of receptor status of breast tumors to be carried out. This antibody stains the nucleus of receptor positive cells.


Concentrated:



Estrogen Receptor (InSite® ER)

Breast carcinoma stained with Anti-ER using DAB chromogen

Clone: ER88

Isotype: IgG1

Source: Mouse

Immunogen: Recombinant Estrogen Receptor protein





Xmatrx HX031-YCDE

AM368-5ME

AM368-10ME

MU368-UCE

FG-368M

FB-368M

P


AR385-10R

Estrogen Receptor-Beta (ER-β)

Human Estrogen Receptor beta (ERb) is highly homologous to human ERa and displays 96% and 58% homology in the DNA and hormone binding domains, respectively. Human ERb mRNA is expressed in testis, prostate, thyroid, ovary, and smooth muscle. ERb is expressed in various normal and neoplastic cells. The rabbit polyclonal antibody ERb88 is directed against human ERb protein and marks nuclei of many different cells on formalin-fixed, paraffin-embedded tissue sections.



Breast carcinoma stained with Anti-ER-beta using DAB chromogen

Clone: Polyclonal

Source: Rabbit

Immunogen: A 17-mer sequence close to carboxy-terminus of ER-β protein was chosen to be the template for synthesis of a 4-branch Multiple Antigenic Peptide (MAP)

Specificity: Estrogen Receptor-β protein





P


97

Primary Antibodies


Factor VIII-Related Antigen

This antigen has proven to be one of the best available immunohistochemical markers for the identification of endothelial cells. Demonstration of Factor VIII-related antigen by immunohistochemical staining has been suggested to identify vascular invasion by neoplasms.


AM016-10M




Leiomyoma stained with Anti-FVIII using AEC chromogen

Clone: F8 2.2.9

Isotype: IgG1 Kappa

Source: Mouse

Immunogen: Purified Human Factor VIII

Specificity: Factor VIII-related antigen




Xmatrx: HX031-YCDE

P


Factor XIII Subunit A

The enzyme Factor XIII is a protransglutaminase involved in the final part of the coagulation pathway, stabilizing clot formation by cross-linking fibronectin to collagen. Factor XIII is found within a variety of dendritic cells in connective tissues. It plays a general role in various processes such as cell proliferation and tissue remodeling, including embryonic and fetal embryogenesis, wound healing, atherosclerosis, and tumor growth. This antibody stains the cytoplasm of positive cells.

AM337-10M



Factor XIII A positivity in placenta stained using AEC chromogen

Clone:

Isotype:

E980.1

IgG1

Source: Mouse

Immunogen: Prokaryotic recombinant protein corresponding to a portion of the C-terminus of factor XIIIa molecule

Specificity: Coagulation Factor XIIIa




Xmatrx HX031-YCDE

P



Factor XIII A

Factor XIII is activated by thrombin and calcium ions to transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-links alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.


AN516-10M



Bone Marrow stained with Anti-Factor XIII A using DAB chromogen

Clone: EP3372

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues in human Factor XIII A.

Specificity: Factor-XIII A




Xmatrx: HX031-YCDE

P


Fascin

Human fascin is a highly conserved actin-bundling protein. Fascin, encoded by the human homolog for the sn (hsn) gene, has been localized to microspikes and stress fibers of cultured cells where it is thought to be involved in the formation of microfilament bundles. It is expressed predominantly in dendritic cells. Lymphoid cells, myeloid cells and plasma cells are negative. However. Reed Sternberg cells in Hodgkin’s lymphoma are positive for fascin staining. Epstein-Barr virus may induce expression of fascin in B cells.

Lymph Node stained with Anti-Fascin using DAB chromogen

Clone: FCN01

Isotype: IgG

Source: Mouse

Immunogen: Fascin purified from HeLa cells

Specificity: Fascin


Pre-treatment: AR-Citra/EZ-AR 1


Xmatrx: HX031-YCDE

P





Primary Antibodies


Fibronectin

Fibronectin is a large heterodimeric glycoprotein composed of two 250kD subunits. It can be found soluble in plasma or insoluble as an extracellular matrix protein. Fibronectin is involved in various cell processes such as cell adhesion, migration, wound healing, and in embryogenesis. The abnormal expression of fibronectin has been implicated with the progression of several cancers. Cellular fibronectin is widely distributed in the stroma of malignant tumours. In non-small cell lung carcinomas (NSCLC), fibronectin expression is upregulated leading to increased tumor growth and apoptosis resistance.

Placenta tissue stained with Anti-Fibronectin using DAB chromogen

Clone: F14

Isotype: IgG

Source: Rabbit

Immunogen: A recombinant protein

Specificity: Fibronectin

Localization: Cytoplasmic and extra-cellular matrix staining





AN569-10M


FG-569N Recommended Positive Control:

P


Follicle Stimulating Hormone (FSH)

Malignant pituitary adenomas or pituitary carcinomas arise from and consist of adenohypophysial cells. They can produce various hormones such as ACTH, growth hormone, TSH, FSH, LH, and prolactin. Tumors that do not consist of adenohypophysial cells neither produce nor contain pituitary hormone, and thus immunoperoxidase techniques are helpful in distinguishing them from those pituitary tumors that store various hormones in the cell cytoplasm. FSH, a glycoprotein hormone, stimulates the graafian follicles of the ovary and assists subsequently in follicular maturation and the secretion of estradiol. In males, it stimulates the epithelium of the seminiferous tubules and is partially responsible for inducing spermatogenesis. This antibody stains of FSH in gonadotrophs.


Pituitary tissue stained with Anti-FSH using AEC chromogen

Clone: Polyclonal

Source: Rabbit

Immunogen: Human pituitary FSH peptide

Specificity: Follicle stimulating hormone (FSH)



Xmatrx: HX031-YCDE

P

Ready-to-use (Automated): Xmatrx® AW026-YCD

Gastrin

The major source of Gastrin in the body is the antropyloric mucosa of the stomach. Significant increases in the antropyloric G-cell (gastrin producers) population occur in a wide variety of clinical conditions such as atrophic gastritis, pernicious anemia, gastric carcinoma, gastric outlet obstruction, Zollinger-Ellison syndrome, and duodenal ulcer disease. Neoplastic proliferations of the gastrin producing cells are frequently associated with the Zollinger-Ellison syndrome.




Stomach tissue stained with Anti-Gastrin using DAB chromogen

Clone: Polyclonal

Source: Rabbit

Immunogen: Synthetic human Gastrin-I bound to keyhole limpet hemocyanin (KLH) with carbodiimide

Specificity: Gastrin



Xmatrx: HX031-YCDE

P


AN481-5M

AN481-10M

NU481-UC

FG-481N

GCDFP-15

The androgen and prolactin responsive prolactin-inducible protein (PIP)/gross cystic disease fluid protein (GCDFP-15) is expressed in human breast tumors and in such normal exocrine organs as sweat, salivary and lacrimal glands. The hormonally responsive prolactin-inducible protein (PIP) gene is expressed in breast tumor tissues and in such normal exocrine organs as sweat, salivary and lacrimal glands.


Concentrated:


Breast Carcinoma stained with Anti-GCDFP-15 using DAB chromogen

Clone: EP1582Y

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues near the N-terminus of human GCDFP-15

Specificity: GCDFP-15




Xmatrx: HX031-YCDE

P


99

Primary Antibodies


Glial Fibrillary Acidic Protein (GFAP)

Glial Fibrillary Acidic Protein (GFAP) is the subunit of the glial specific “intermediate” filament that includes desmin filaments in smooth muscle, vimentin filaments in cultured fibroblasts, keratin filaments in epithelium and neurofilaments in neural cells. This antibody stains human GFAP in positive astrocytes and other positive cells.


AM020-10M




Astrocytes and other acidic fibers in cerebrum stained with Anti-GFAP using DAB chromogen

Clone: GA-5

Isotype: IgG1

Source: Mouse

Immunogen: GFAP isolated from porcine spinal cord

Specificity: Glial fibrillary acidic protein (GFAP)


Pre-treatment: None

P



Glial Fibrillary Acidic Protein (GFAP) is the subunit of the glial specific “intermediate” filament that includes desmin filaments in smooth muscle, vimentin filaments in cultured fibroblasts, keratin filaments in epithelium and neurofilaments in neural cells. This antibody stains GFAP from many species including human, mouse, and rat in cytoplasm of astrocytes and Bergmann glia.


AR020-10R


Cerebellum tissue stained with Anti-GFAP using DAB chromogen

Clone: Polyclonal

Source: Rabbit

Immunogen: GFAP isolated from bovine spinal cord.

Specificity: Glial fibrillary acidic protein (GFAP)



Manual/i6000: None

Xmatrx: HX031-YCDE

P



Glomerular Epithelial Protein 1 (GLEPP-1)

Glomerular epithelial protein 1 (GLEPP1) is a transmembrane protein tyrosine phosphatase found only in the epithelial cells of the renal glomerulus.The monoclonal antibody 5C11 has been raised against a fusion protein made from part of its extracellular domain. In the normal glomerulus, GLEPP1 is present only in visceral glomerular cells (podocytes). The presence of GLEPP1 may be used as a marker of podocyte integrity in various forms of glomerular injury. This antibody stains human GLEPP1 protein in the epithelial cells of the renal glomerulus.



Kidney tissue stained with Anti-GLEPP-1 using AEC chromogen

Clone: 5C11

Isotype: IgG2b

Source: Mouse

Immunogen: GLEPP-1 fusion protein

Specificity: GLEPP1



Manual: HK058-5KE


P


Glucagon

Glucagon is a polypeptide of 29 amino acids produced by the pancreatic alpha cells. In addition to its well known effect of elevating blood glucose concentration, glucagon functions to inhibit gastric and pancreatic secretions. Tumors producing large amounts of glucagon are referred to as glucagonomas. This antibody stains the cytoplasm in A cells of the endocrine pancreas and reacts with glucagon in a number of mammalian species.

Pancreas tissue stained with Anti-Glucagon using AEC chromogen

Clone: Polyclonal

Source: Rabbit

Immunogen: Synthetic human glucagon

Specificity: Glucagon



Xmatrx: HX031-YCDE

P





Primary Antibodies


Glut-1

Glucose is fundamental to the metabolism in mammalian cells. Several glucose transporter protein (Glut) isoforms have been identified and shown to function in response to insulin and IGF-1 induced signaling. GLUT-1 is detectable in many human tissues including those of the colon, lung, stomach, esophagus, and breast. GLUT-1 immunoreactivity in some cancers, including trans carcinoma of the urinary bladder, has been associated with aggressive behavior.


AM505-10M



Kidney tissue stained with Anti-Glut-1 using DAB chromogen

Clone: SPM498

Isotype: IgG

Source: Mouse

Immunogen: Glut-1 purified from Primary cultures of myoblasts.

Specificity: GLUT-1





P


Glutathione S-Transferase Pi (GST Pi)

Glutathione S-Transferases (GSTs) are a multigene family of enzymes centrally involved with drug metabolism and detoxification. All eukaryotic species possess multiple cytosolic and membrane-bound GST isoenzymes, each of which displays distinct catalytic as well as noncatalytic binding properties.

GST Pi positivity in breast carcinoma stained using DAB chromogen





Clone: Polyclonal

Source: Rabbit

Immunogen: Purified proteins from the cytosol of a human chronic lymphoblastic spleen

Specificity: Glutathione S-transferase pi




P

Glypican-3 (GPC3)

Glypican-3 (GPC3) is a glycosylphospatidyl inositol-anchored membrane protein, which may also be found in a secreted form. GPC3 belongs to the glypican family of heparan sulfate proteoglycans. This protein may be involved in the suppression/modulation of growth in the predominantly mesodermal tissues and organs. Glypican-3 is thought to regulate tissue and organ growth through interactions with growth factors such as insulin-like growth factor II or fibroblast growth factor 2. For research use only. Not for use in diagnostic procedures.


AM539-10M



Hepatocellular carcinoma stained with Anti-Glypican-3 using DAB chromogen

Clone: GPC3-88

Isotype: IgG

Source: Mouse

Immunogen: Glypican-3 is a mouse monoclonal antibody derived from cell culture supernatant

Specificity: Glypican




P

Ready-to-use (Automated): i6000TM

RUO

Granulocyte

The BM-2 antibody can provide important differentiation information and may be used along with antibodies BM-1 and BM-3 to stain early precursor and mature forms of human myeloid cells. This group of monoclonal antibodies reacts with antigenic determinants present in normal myeloid cells and leukemias of similar derivation. BM-2 recognizes an antigen present in the cytoplasm of mature granulocytes. This antibody stains the cytoplasm of human granulocytes (polymorphonuclear leukocytes) residing in lymphoid and non-lymphoid tissue.



Bone marrow trephine stained with Anti-Granulocyte using AEC chromogen

Clone: BM-2

Isotype: IgG1

Source: Mouse

Immunogen: Nuclei from pokeweed mitogen-stimulated human peripheral blood lymphocytes

Specificity: Granulocytes


P

101

Primary Antibodies


Growth Hormone (hGH)

Growth Hormone (GH, somatotropin) is the primary hormone responsible for regulating overall body growth and is also important in organic metabolism. It is synthesized by acidophilic or somatotropic cells of the anterior pituitary gland. Human GH has a molecular weight of 22 kD. GH stimulates growth indirectly by promoting the liver’s production of somatomedins, which act directly on bone and soft tissue to cause growth. GH exerts direct metabolic effects on the liver, adipose tissue and muscle. In general, growth hormone enhances protein synthesis, conserves carbohydrates and uses up fat stores.





Placenta stained with anti-HGH antibody using DAB chromogen

P

AR707-10R AW707-YCD


Xmatrx® :

Clone: Polyclonal

Isotype: IgG

Source: Rabbit

Immunogen: HGH

Specificity: HGH




Xmatrx: HX031-YCDE

Heat Shock Protein (HSP-70)

HSP-70 is a member of a multigene family encoding several closely related 70-73 kD stress proteins (the HSP-70 family). These genes differ in their intracellular location and regulation and are thought to be involved in protein-protein interactions such as those of the protein products of the p53 tumor suppressor gene and the human c-myc oncogene. This antibody stains HSP-70 localized in the cytoplasm and/or nuclei in tissue from breast carcinoma, brain tumors, Alzheimer's disease and alcoholic liver disease.




Breast carcinoma stained with Anti-HSP 70 using DAB chromogen

Clone: BRM-22

Isotype: IgG1

Source: Mouse

Immunogen: Bovine brain HSP70

Specificity: Heat Shock Protein70 (HSP-70)




P


Heat Shock Protein 27 (HSP 27)

HSP27 also known as the 24K estrogen-regulated protein or HSP28, is a small heat shock protein that has been shown to correlate with the expression of estrogen-receptors. Increased levels of HSP27 have been shown to correlate with the presence of ER and PR in human breast tumor biopsy samples. This antibody stains estrogen regulated heat shock protein (HSP27) in cytoplasm of cells in female reproductive tract.





Breast carcinoma stained with Anti-HSP27 using AEC chromogen

Clone: G3.1

Isotype: IgG1

Source: Mouse

Immunogen: Balb/c mice were immunized with "24K" protein isolated from the cytosol of MCF-7 cells. Spleen cells from immunized mice were fused with NS-1 myeloma cells

Specificity: hsp27




Xmatrx: HX031-YCDE

P


PHelicobacter pylori

H. pylori is associated with B-type gastritis and some duodenal ulcers. Studies conducted by various researchers have shown that immunostaining for H. pylori with monoclonal antibodies is more reliable and easier to read than traditional H&E staining and significantly more sensitive than the Warthin-Starry silver stain, which is very complicated to perform. This antibody stains the bacilli in lumen of infected stomach in formalin-fixed, paraffin-embedded tissue sections.

Concentrated: PU442-UPE


Infected mucosa stained with Anti-H. pylori using AEC chromogen

Clone: Polyclonal

Source: Rabbit

Immunogen: Heat killed bacteria

Specificity: Helicobacter pylori

Localization: Bacterium

Pre-treatment: AR 10/EZ-AR 1/EZ-AR 2

Manual: HK058-5K


AR422-10RE


Ready-to-use (Automated): i6000TM Xmatrx® AW442-YCDE

Primary Antibodies


Hemoglobin A

Immunohistochemical localization of hemoglobin is an excellent marker for the detection of immature, dysplastic, and megaloblastic erythroid cells particularly in myeloproliferative disorders such as erythroleukemia. Myeloid cells, lymphoid cells, plasma cells, histiocytes and megakaryocytes do not give positive staining for hemoglobin. Megaloblastic erythroid cells give strong staining for hemoglobin. This antibody stains human hemoglobin A predominantly in cytoplasm of erythroid cells.



Placenta tissue stained with Anti-Hb using AEC chromogen

Clone: Polyclonal

Source: Rabbit

Immunogen: Purified hemoglobin

Specificity: Hemoglobin A



Xmatrx: HX031-YCDE

P


Hepatitis B Virus Core Antigen (HBcAg)

HBcAg is the designation given to the antigenic activity of the 28 nm nucleocapsid core of Hepatitis B Virus. Immunocytochemical staining for HBcAg can provide conclusive evidence for a viral etiology in hepatitis. Furthermore, viral antigenic expression in liver cells can be correlated with the histopathologic changes, thus facilitating investigation into the mechanism of virus-induced injury. HBcAg is not detected in the serum of either acutely or chronically infected individuals. As the patient becomes symptomatic, HBc antibody becomes detectable. This antibody stains Hepatitis B Virus Core Antigen in nuclei of infected cells in tissue sections stained by immunohistochemical techniques. For research use only. Not for use in diagnostic procedures.


Concentrated:

Liver tissue stained with Anti-HBcAg using AEC chromogen

Clone: Polyclonal

Source: Rabbit

Immunogen: HBcAg purified from lysates of E. coli clones containing the viral core DNA

Specificity: Hepatitis B core antigen


P

AR082-5RE

PU082-UPE

RUO

FG-082PRecommended Positive Control:Recommended Barrier Control: FB-082P

Herpes Simplex Virus Type I (HSV I)

Human herpes simplex virus type I (HSV-I) is part of the Herpesvirus family which also includes HSV-II, Epstein-Barr virus (mononucleosis), herpes zoster (chicken pox) and cytomegalovirus. They grow in the cell nuclei, bud through the nuclear membrane and cause latent infections. HSV are among the most common infectious agents of man, usually transmitted through close personal contact. HSV-I is responsible for causing gingivostomatitis or “fever blisters.” There is a significant degree of cross-reactivity between HSV-I and HSV-II. No cross-reactivity is seen with the Epstein-Barr virus, cytomegalovirus or herpes zooster virus.


PU084-UPEConcentrated:

Infected lung tissue stained with Anti-HSV I using AEC chromogen

Clone: Polyclonal

Source: Rabbit

Immunogen: Rabbit cornea cells infected with the MacIntyre strain of HSV type I and solubilized in detergent

Specificity: Herpes Simplex Virus (HSV) type I




P


Herpes Simplex Virus Type II (HSV II)

HSV II is the virus known for causing Herpes genitalis. The antibody reacts with all the major glycoproteins present in the viral envelope and at least one core protein as determined by crossed immunoelectrophoresis. It does not cross react with cytomegalovirus and Epstein-Barr virus. This antibody detects HSV in human cytological samples obtained from superficial lesions or biopsies and infected tissue cultures.



Cultured cells infected with HSV II, stained with Anti HSV II using DAB chromogen

Clone: Polyclonal

Source: Rabbit

Immunogen: HSV type II (strain MS) infected whole rabbit cornea cells solubilized in detergent

Specificity: Herpes simplex Virus (HSV) type II



Xmatrx: HX032-YCDE

P


103

Primary Antibodies


HLA-DR

HLA-DR is a transmembrane glycoprotein composed of an alpha chain (36 kD) and a beta chain (27 kD). LN3 is reactive with a non-polymorphic antigen of the HLA-DR (Ia) region, expressed primarily by antigen presenting cells, B-cells of the germinal centers and mantle zones, and additionally by monocytes, macrophages and interdigitating histiocytes. LN3 will produce medium intensity staining on B lymphocytes of germinal centers and mantle zones, and high intensity staining of interdigitating histiocytes in T-cell zones. This antibody stains the HLA-DR antigen in membrane of positive cells. For research use only. Not for use in diagnostic procedures.




Thyroid tissue stained with Anti-HLADR using DAB chromogen

Clone: LN3

Isotype: IgG2a

Source: Mouse

Immunogen: Activated human peripheral blood mononuclear cells

Specificity: LN3




P

Ready-to-use (Automated): Xmatrx® AX154-YCDE

RUO

HSA

Hepatocyte Specific Antigen (HSA) has been demonstrated consistently in the vast majority of hepatocellular carcinomas. HSA recognizes both benign and malignant liver derived tissues including such tumors as hepatoblastoma, Hepatocellular carcinoma, and hepatic adenoma. It recognizes both normal adult and fetal liver tissue. This antibody is useful in differentiating hepatocellular carcinomas with adenoid features from adenocarcinomas, either primary in the liver or metastatic lesions to the liver. In recognizing hepatoblastoma, it is useful in differentiating this entity from other small round cell tumors.


AM550-10M


Liver tissue stained with Anti-HSA using DAB as a chromogen

Clone: HSA/E8

Isotype: IgG1/K

Source: Mouse

Immunogen: Human HSA

Specificity: HSA





P


Human Chorionic Gonadotropin (hCG) Beta

Human Chorionic Gonadotropin (hCG) is a 40 kD glycoprotein secreted in large quantities by the placenta and normally circulates at readily detectable levels only during gestation. Immunohistochemical studies reveal localization of hCG in syncytiotrophoblasts. Isolated clusters of giant cells may be found in association with certain components of germ cell tumors but are most frequently associated with embryonic carcinoma, endodermal sinus tumor, and germinoma. This antibody stains the cytoplasm of positive cells.



Placenta tissue stained with Anti-hCG beta using DAB chromogen

Clone: M94138

Isotype: IgG

Source: Mouse

Immunogen: Purified hCG Beta-subunit

Specificity: Beta-hCG



Manual HK081-5KE

Xmatrx HX031-YCDE

P


IgA

IgA is the predominant antibody isotype in mucosal areas. This antibody reacts with IgA but not with other isotypes. It is useful in the evaluation of leukemias, plasmacytomas, certain non-Hodgkin’s lymphomas, and glomerulonephritis.

Tonsil tissue stained with anti-IgA using AEC chromogen

Clone: Polyclonal

Source: Rabbit

Immunogen: IgA isolated from human serum

Specificity: IgA




P




Primary Antibodies


IgD

IgD is expressed on mature B cells and may be used to classify B cell neoplasms. Mantle zone B-cells in primary follicles and those outlining the germinal centers of secondary follicles are seen to be positive for IgD expression. Thus, this antibody could be used to detect changes in nodal architecture. It also may be used to detect the expanded follicular structures of progressive transformation of germinal center (PTGC), which are composed largely of IgD+ mantle zone B-cells. It is used along with IgM as a marker to identify marginal zone lymphomas.




Clone: Polyclonal

Source: Rabbit

Immunogen: IgD isolated from a pool of normal human sera

Specificity: Human IgD



Manual: HK058-5K


P

Tonsil stained with Anti-IgD using AEC chromogen


IgG

The molecular weight of IgG is 150 kD consisting of two gamma heavy chains and two kappa or lambda light chains. Immunohistochemical techniques to identify immunoglobulins have been used in the classification of leukemias, plasmacytomas and certain non-Hodgkin’s lymphomas. In addition, immunoglobulin immunohistochemistry has been widely used in nephropathology and dermatopathology for studying a variety of immune diseases.




Tonsil stained with Anti-IgG using AEC chromogen

Clone: IgG88

Isotype: IgG1 Kappa

Source: Mouse

Immunogen: Purified human immunoglobulin

Specificity: IgG




P


IgG

The human B-lymphocyte is characterized by the presence of readily detectable surface immunoglobulins. Up to 10 percent of peripheral blood lymphocytes and 68-70 percent of the lymphocytes in lymph nodes are of the B-cell type. The patterns of reactivity to IgG, IgA, IgM, C3, kappa, and lambda light chains can be used for the characterization of certain kinds of kidney and skin diseases. This antibody stains human IgG in cytoplasm and membrane of B-cells and is negative for light chains and other heavy chains.




Tonsil tissue stained with Anti-IgG using AEC chromogen

Clone: Polyclonal

Source: Rabbit

Immunogen: IgG isolated from human serum

Specificity: IgG




P


IgM

This monoclonal antibody reacts with human IgM heavy (mu) chain Fc region of 900kD pentameric IgM. It does not react with IgA, IgG or with light chains. This antibody stains plasma cells containing IgM, but does not usually stain immune complexes and surface IgM. It is useful for the evaluation of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas, the majority of which derive from B-cell lineage. The common underlying feature of these malignancies is the restricted expression of heavy and light chains to a single heavy and light chain type.




Tonsil stained with Anti-IgM using DAB chromogen

Clone: IgM88

Isotype: IgG 2b Kappa

Source: Mouse

Immunogen: Purified human IgM

Specificity: IgM



Manual/Xmatrx: EK000-5K

P


105

Primary Antibodies


IgM

This polyclonal antibody reacts with mu-chains of human IgM. All B-cells have IgD and IgM expressed predominantly on the surface and presumably act as antigen receptors. Surface IgM is present on mantle zone and marginal zone B-cells. Immature B-cells in bone marrow express IgM and mature B-cells migrating to periphery secrete IgD and IgM. The demonstration of both IgM and IgD can be useful in determining if a B-cell lymphoma is derived from mantle or marginal zone.


AR427-10R




IgM expression in tonsil stained using DAB chromogen

Clone: Polyclonal

Source: Rabbit

Immunogen: IgM isolated from human plasma

Specificity: IgM antigen





P


Inhibin Alpha

Inhibins are dimeric gonadal protein hormones that negatively regulate pituitary FSH synthesis and secretion. Inhibin contains an alpha and beta subunit linked by disulfide bonds. Two forms of inhibin differ in their beta subunits (A or B), while their alpha subunits are identical. Inhibin B is comprised of the Inhibin alpha subunit disulfide linked to the Inhibin beta subunit. Initial studies indicated that Inhibin is a critical negative regulator of gonadal stromal cell proliferation and was the first secreted protein identified to have tumor-suppressor activity. Inhibin alpha-subunit immunoreactivity has been detected in Sertoli cells, spermatocytes, and in some Leydig cells.




Ovary tissue stained with Anti-Inhibin Alpha using DAB chromogen

Clone: R1

Isotype: IgG2a

Source: Mouse

Immunogen: Synthetic peptide from 1-32 peptide of the alpha subunit of human Inhibin alpha

Specificity: Inhibin Alpha



Manual: HK081-5KE

Xmatrx: HX031-YCDE

P


AX446-YCD

Insulin

Lack of this hormone gives rise to diabetes mellitus. The development of specific antibodies to various polypeptide hormones have made IHC localization of these hormones such as Insulin (which is produced in the pancreas by beta cells of Islet of Langerhans) the most sensitive and reliable means available for an accurate characterization of the function of islet cell tumors. This antibody recognizes the A chain loop of human Insulin. Cross-reactivity with bovine, rat and mouse Insulin has been observed. This antibody stains insulin in cytoplasm of beta cells in pancreas.

Pancreas tissue stained with Anti-Insulin using AEC chromogen

Clone: HB125

Isotype: IgG 1, Kappa

Source: Mouse

Immunogen: Purified human insulin

Specificity: Insulin



Xmatrx: HX031-YCDE

P





Insulin

Insulin is a hormone that regulates glucose homeostasis. It is synthesized in the pancreas within the β-cells of the islets of Langerhans. One million to three million islets of Langerhans (pancreatic islets) form the endocrine part of the pancreas, which is primarily an exocrine gland. The endocrine portion accounts for only 2% of the total mass of the pancreas. Within the islets of Langerhans, beta cells constitute 65–80% of all the cells. The antibody labels both normal and neoplastic insulin-producing cells. It is useful in identifying insulinoma.

Insulin Pancreas stained with anti-Insulin using DAB chromogen

Clone: EP125

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues in human Insulin protein

Specificity: Human Insulin protein


Pre-treatment: None


AN735-10M



P



Primary Antibodies


J Chain

J chain is a small, glycopeptide of 15 kD. It is structurally unrelated to heavy or light chains, but is synthesized by all plasma cells that secrete polymeric immunoglobulins. J chains are present in a large proportion of the immunoglobulin-positive cells in the germinal centers of the tonsils and lymph nodes. B cells secrete J chain at an early stage of differentiation with the expression persisting in those cells destined to produce IgA or IgM.



Tonsil stained with Anti-J-chain using Fast Red chromogen

Clone: JC88

Isotype: IgG 1 Kappa

Source: Mouse

Immunogen: Human J chain

Specificity: J chain



Xmatrx HX031-YCDE

P


Kappa Light Chain

The light chains of immunoglobulin molecules have two antigenic types: kappa and lambda. A given immunoglobulin molecule contains two light chains, either both kappa or both lambda. As a result the clonal nature of any immunoglobulin-producing cell population can be determined by its light chain structure. The most important use of this technique would be in distinguishing atypical reactive follicular lymphoid hyperplasia from follicular lymphoma, undifferentiated carcinoma from large cell lymphoma, pseudolymphoma from lymphoma, and reactive plasmacytosis from well differentiated plasmacytoma.


AM048-10M



Tonsil stained with Anti-Kappa Light Chain using AEC chromogen

Clone: L1C1

Isotype: IgG1

Source: Mouse

Immunogen: B-lymphoma cells

Specificity: Kappa light chain



Manual/i6000: None

Xmatrx: HX031-YCDE

P


AM369-5M

AM369-10M

MU369-UCFG-369MFB-369M

Kappa Light Chain

This antibody reacts specifically with the kappa light chain of human immunoglobulin and not lambda light chain and is reactive with intact IgG (kappa), IgM (kappa), free kappa light chains, and Bence-Jones kappa light chains. The most important use of this antibody would be in distinguishing atypical reactive follicular lymphoid hyperplasia from follicular lymphoma, undifferentiated carcinoma from large cell lymphoma, pseudolymphoma from lymphoma, and reactive plasmacytosis from well differentiated plasmacytoma.


Concentrated:Recommended Positive Control:Recommended Barrier Control:

Plasma cell in the tonsil showing Kappa light chain positivity stained using DAB chromogen

Clone: K88


Source: Mouse

Immunogen: Human kappa protein

Specificity: Kappa light chain



Manual/i6000: None

Xmatrx: HX031-YCDE

P


Ki-67

Ki-67 reacts with a human nuclear antigen that is expressed in proliferating cells but not in resting cells. Ki-67 antigen is a potent tool for rapidly evaluating the growth fraction of any given human cell subset. It is particularly useful in studying malignant tumors and other pathogenic states as a measure of the proportion of proliferating cells. Immunostaining of Ki-67 antigen in normal tissue shows nuclear reactivity in cells of germinal centers of cortical follicles, cortical thymocytes, neck cells of gastrointestinal mucosa, and undifferentiated spermatogonia.


AM410-10MAX410-YCD




Medulloblastoma stained with Anti-Ki-67 using DAB chromogen

Clone: K-2

Isotype: IgG1 Kappa

Source: Mouse

Immunogen: Recombinant Ki-67 protein fragment close to C-terminus

Specificity: Ki-67 antigen




Xmatrx: HX032-YCDE

P


107

Primary Antibodies


Ki-67

Ki-67 antigen is a nuclear antigen specifically associated with cell proliferation. Ki-67 is expressed in all proliferating cells which are in the active phases of the cell cycle (late G1, S, G2, and mitosis), but absent in resting cells. In G1, it is predominately localized in the perinucleolar region and also found in the nuclear matrix in the later phases. Ki-67 is thought to be involved in the maintaining of cell proliferation; it is considered an important marker in human tumor diagnostics and prognostics.


AN527-10M



Tonsil stained with Anti-KI67 using DAB chromogen

Clone: EPR3611

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues in human Ki-67.

Specificity: Ki-67


Pre-treatment: EZ-ARTM2


Xmatrx: HX032-YCDE

P


Ki-67

Ki-67 antigen is a nuclear antigen specifically associated with cell proliferation. Ki-67 is expressed in all proliferating cells which are in the active phases of the cell cycle (late G1, S, G2, and mitosis), but is absent from resting cells (G0). It is strictly associated with cell proliferation. Ki-67 labeling index has been shown to be elevated in early stage and further increased in advanced stage of various types of cancer including breast cancer, colon cancer, prostate cancer and brain cancer.

Tonsil stained with anti-ki67 antibody using DAB chromogen

Clone: EP5

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues in human Ki-67 protein

Specificity: Ki-67




Xmatrx None


AN727-10M



P



Ki-67 Antigen, Proliferating Cell

Ki-67 is one of the most widely studied proliferating cell antigens. The expression of Ki-67 antigen is limited to cells in phase G1, S and G2 with the highest levels present in the M phase. Ki-67 is more likely to be expressed in aneuploid tumors compared to diploid tumors, and it is associated with a high mitotic count and high histology grade.This monoclonal antibody enables detection of Ki-67 in proliferating cell populations in routine paraffin sections. The antibody stains positive in the nucleus of proliferation cells.


AM297-10MAX297-YCD



Tonsil stained with Anti-KI67 using AEC chromogen

Clone: MIB-1

Isotype: IgG1

Source: Mouse

Immunogen: Peptide fragment of Ki-67 antigen





Xmatrx: HX032-YCDE

P


Ki-67 Antigen, Proliferating Cell

The monoclonal antibody Ki88 reacts with a human nuclear antigen expressed in proliferating cells but absent in resting cells. Immunostaining of the Ki-67 the antigen in normal tissue shows nuclear reactivity in cells of germinal centers of cortical follicles, cortical thymocytes, neck cells of gastrointestinal mucosa, and undifferentiated spermatogonia. Resting cells such as lymphocytes, monocytes, parietal cells and Paneth’s cells of gastrointestinal mucosa, hepatocytes, renal cells, and mature sperm cells do not stain. This antibody stains a human nuclear antigen expressed in all proliferating cells.

Lymph node germinal cells stained with Anti-Ki-67 using DAB chromogen

Clone: Ki88


Source: Mouse

Immunogen: Recombinant human Ki-67 protein





Xmatrx: HX032-YCDE

P


AM370-10MAX370-YCD





Primary Antibodies


Lambda Light Chain

The light chains of immunoglobulin molecules may be either Kappa or Lambda. Antibodies to kappa and lambda light chains are used for the evaluation of leukemias, plasmacytomas, and certain non-Hodgkin’s lymphomas, the majority of which are derived from B-cell lineage. The most important uses of this technique would be in distinguishing atypical reactive follicular lymphoid hyperplasia from follicular lymphoma, undifferentiated carcinoma from large cell lymphoma, pseudolymphoma from lymphoma, and reactive plasmacytosis from well differentiated plasmacytoma.




Tonsil stained with Anti-lambda light chain using DAB chromogen

Clone: Polyclonal

Source: Rabbit

Immunogen: Pool of human lambda Bence Jones proteins

Specificity: Lambda light chains



Xmatrx: HX031-YCDE

P


Lambda Light Chain

The basic structure of an immunoglobulin molecule consists of two identical heavy chains, either γ, µ, α, δ or ε and two identical light chains, either kappa or lambda.

The gene rearrangement process that generates the immunoglobulin molecule results in either a productive kappa or lambda gene. The ratio of kappa and lambda light chains varies between Ig classes and subclasses. The lambda light chain antibody labels the lambda light chain that expresses normal and neoplastic B lymphocytes and plasma cells. Other cells may also express lambda light chain due to nonspecific uptake of immunoglobulin. The occurrence of a mixture of kappa and lambda chain expressing cells suggests a polyclonal population and a reactive or non-neoplastic proliferation of B cells.

Tonsil stained with anti-Lambda using DAB chromogen

Clone: EP172

Isotype: IgG

Source: Rabbit

Immunogen: A recombinant protein fragment corresponding to human IgA protein

Specificity: Human IgA protein




Xmatrx HX032-YCDE


AN715-10M



P



Laminin

The antibody to laminin selectively and specifically recognizes basement membrane components. Laminin consists of a 220 kD subunit, which is disulfide-linked into larger complexes or even into the structural scaffolding of the basement membrane itself. In surgical pathology, laminin can be used as a marker to demonstrate morphologic change of basement membrane, which is helpful for interpreting the invasion of malignant tumors. Laminin could also be used to study histogenesis and pathogenesis of certain unknown lesions such as extracellular and intracellular hyaline bodies occurring in various diseases. This antibody stains Laminin in basement membranes.


AR078-10R




Lung stained with Anti-laminin using DAB chromogen

Clone: Polyclonal

Source: Rabbit

Immunogen: Laminin isolated from EHS-mouse sarcoma

Specificity: Laminin

Localization: Basement Membrane

Pre-treatment: Pepsin, RT, 10 min. /EZ-AR 1/EZ-AR 2



P


Lysozyme

Lysozyme (also called muramidase) is an enzyme which acts on bacterial cell walls by cleaving N-acetyl-glucosaminyl-N-acetylmuramic acid linkages. Lysozyme is present in human milk, tears, saliva, and serum. It is also found in myeloid cells, monocytes and histiocytes, making it useful for the demonstration of the myeloid or monocytic nature of acute leukemia. This antibody stains the cytoplasm of granulocytes and monocytes/macrophages.

Lymph Node stained with anti-lysozyme using AEC chromogen

Clone: Polyclonal

Source: Rabbit

Immunogen: Lysozyme isolated from the urine of monocytic leukemia patients

Specificity: Lysozyme



Xmatrx: HX031-YCDE

P


AR024-10R





109

Primary Antibodies


Macrophage

LN5 stains an unidentified antigen in cytoplasm of macrophages and histiocytes in hematopoietic organs. It stains mantle zone B lymphocytes of the lymph node and spleen, spermatogonia, chief cells of the stomach, ductal epithelium of breast and tubular epithelium of kidney. It is strongly reactive with cases of true histiocytic lymphoma but is negative, except for macrophages, in Hodgkins disease and non-Hodgkins lymphomas. It can be an important tool for the study of malignant and benign histiocytic lesions. This antibody stains the cytoplasm of a specific population of human macrophage and histiocytes.




Kupffer cells stained with anti-Macrophage using DAB chromogen

Clone: LN5

Isotype: IgM

Source: Mouse

Immunogen: Human peripheral blood cells

Specificity: Macrophages



Xmatrx: HX031-YCDE

P


Mast Cell Tryptase

The monoclonal antibody produced by clone AA1 reacts with human Mast Cell Tryptase in different tissues. Relatively high levels of the enzyme are found in mast cells of skin and lung. Tryptase, a structurally unique trypsin like serine protease, is a biochemical marker that has proven useful for disorders that involve systemic mast cell activation. It is shown to be implicated as a potential mediator in the pathology of several mast cell related allergic and inflammatory conditions, including rhinitis, conjunctivitis, and most notably asthma. This antibody stains Mast Cell Tryptase antigen in cytoplasm of mast cells in skin, lung and tonsil tissues.


MU419-UC

FG-419M

Concentrated:

FB-419M



AM419-10M

Clone: AA1

Isotype: IgG1

Source: Mouse

Immunogen: Human Mast Cell Tryptase purified from human lung tissue

Specificity: Mast Cell Tryptase antigen



Manual/i6000: None

Xmatrx: HX031-YCDE

Mast cell in the dermis highlighted by Mast Cell Tryptase antibody using AEC chromogen

P


Melan-A (MART-1)

Melan-A, a product of the MART-1 gene, is a differentiation antigen which is expressed in 100% of melanocytes, most melanomas, and 50-60% of melanoma cell lines. It is one of the melanoma antigens recognized by autologous cytotoxic T cells, and as an antigenic target for tumor infiltrating lymphocytes. This antibody also stains Melan-A in normal melanocytes and in the retina. It does not stain normal or tumor tissues from non-melanocyte lineages. This antibody stains positive in cytoplasm of melanocytes and other positive cells.


AM361-10M



Clone: A103

Isotype: IgG

Source: Mouse

Immunogen: Recombinant Melan-A protein

Specificity: Melan-A or MART-1




Xmatrx: HX031-YCDEMelanoma stained with Anti-Melan-A using DAB chromogen

P



Melanoma

Metastatic melanoma is often confused with a variety of poorly differentiated carcinomas, sarcomas, and large cell lymphomas. Clone HMB45 reacts with fetal and neonatal melanocytes but not with normal adult melanocytes and junctional nevus cells but not with intradermal nevi, hence showing specificity for detection of melanocytic tumors. The panel of tumor markers, most commonly used in conjunction with HMB45, for evaluation of melanoma includes S-100 protein LCA, CEA, and EMA, as well as vimentin, an intermediate filament found in both melanomas and sarcomas.


AM001-10M




Melanoma tumor cells positive for melanoma antigen stained using AEC chromogen

Clone: HMB45

Isotype: IgG1

Source: Mouse

Immunogen: Metastatic malignant melanoma cells

Specificity: Malignant melanoma




Xmatrx: HX031-YCDE

P


Primary Antibodies


Melanoma gp100

Melanoma gp100/D5 is a mouse monoclonal antibody that reacts against an antigen present in melanocytic tumors such as melanomas. It reacted positively against melanocytic tumors but not other tumors, thus demonstrating specificity and sensitivity. This antibody is very useful to identify malignant melanoma. Metastatic amelanotic melanoma can often be confused with a variety of poorly differentiated carcinomas, large cell lymphomas, sarcomas, spindle cell carcinomas and various types of mesenchymal neoplasms.

Melanoma tissue stained with Anti-Melanoma gp100 using AEC chromogen

Clone: gp100/D5

Isotype: IgG1/K

Source: Mouse

Immunogen: Human melanoma gp100

Specificity: Melanoma gp100


Pre-treatment: AR Citra Solution/EZ-AR 1


Xmatrx: HX031-YCDE


AM536-10M



P


AM077-5M

AM077-10M

MU077-UC

FG-077M

FB-077M

Melanoma-Associated Antigen

The melanoma associated antigen is a formalin resistant glycoprotein with a disulphide dependent configuration that is essential for recognition by the NKI/C3 monoclonal antibody. This antibody recognizes a heterogeneous 25-110 kD glycoprotein that is located mainly in the inner side of membranes of cytoplasmic vesicles in melanoma cells. This antibody reacts with melanoma, nevocellular nevi, carcinoids and medullary carcinomas of the thyroid. It does not react with basal cell carcinoma, brain tissue or brain tumors.


Concentrated:



Melanoma stained with Anti-Melanoma associated antigen using DAB chromogen

Clone: NKI/C3

Isotype: IgG1

Source: Mouse

Immunogen: Purified membranes of human melanoma cells

Specificity: NKI/C3 antigen



Manual/i6000: None

Xmatrx: HX031-YCD/EHX032-YCDE

P


Mesothelin

Mesothelin, a 40kD glycosyl-phosphatidylinositol–linked cell surface glycoprotein, is present on the surface of the mesothelial cells and may be involved in cell adhesion. It is also seen on mesotheliomas, epithelial ovarian cancers, and some squamous cell carcinomas. Clone 5B2 reactivity has been seen in epitheloid mesotheliomas and adenocarcinomas of lung, ovary, peritoneum, endometrium, pancreas, stomach and colon to a varying degree. Mesothelin is abundant in normal mesothelial cells from which malignant mesotheliomas and ovarian cystadeno carcinomas are derived. This antibody can be used in conjunction with an antibody to calretinin for evaluation of mesotheliomas.


AM433-10M



Clone: 5B2

Isotype: IgG1

Source: Mouse

Immunogen: Prokaryotic recombinant fusion protein corresponding to approximately 100 amino acids from membrane bound form of mesothelin.

Specificity: Mesothelin




Xmatrx: HX031-YCDE

Ovary adenoma stained with anti-Mesothelin using DAB chromogen

P


MiTF

Micropthalmia-associated Transcription Factor (MiTF) is a basic helix-loop-helix leucine zipper transcription factor involved in melanocyte and osteoclast development. Mutations in MiTF cause auditory pigmentary syndromes, such as Waardenburg Syndrome Type II, Type IIa and Tietz Syndrome in humans. MiTF plays a critical role in the differentiation of various cell types such as neural crest-derived melanocytes, mast cells, osteoclasts and optic cup-derived retinal pigment epithelium. This antibody recognizes serine phosphorylated and non-phosphorylated melanocytic isoforms of micropthalmia. It is useful in identifying malignant melanoma, and distinguishing mast cell lesions of myeloid derivation. A relatively rare class of tumors known as PEComas (tumors showing perivascular epitheloid cell differentiation) express MiTF in a high percentage of cases ~90%).

Melanoma tissue stained with Anti-MiTF using AEC chromogen

Clone: MiTF/A13

Isotype: IgG1/k

Source: Mouse

Immunogen: Human MiTF

Specificity: MiTF




Xmatrx: HX032-YCDE


AM554-10M



P


111

Primary Antibodies


Mitochondrial Antigen

Monoclonal antibody 113-1 recognizes a 60 kD antigen of human mitochondria. This marker may be useful in identification of mitochondria in cells, tissues, and biochemical preparations. It produces a “spaghetti-like” staining pattern in the cytoplasm of human cells and may be used as a marker of biliary cirrhosis. The antibody stains mitochondria in cytoplasm of positive cells.

Concentrated:

Fetal Liver tissue stained with Anti-Mitochondrial Ag using AEC chromogen

Clone: 113-1

Isotype: IgG1

Source: Mouse

Immunogen: Raji Burkitt's lymphoma cells

Specificity: Mitochondria



Manual: HK087-5KE

MU213-UC

P

Mismatch Protein Repair (MLH1)

MLH1 is a mismatch repair protein involved in maintaining the integrity of genetic information alongside MSH2. MSH6 and PMS2. During DNA replication, strand misalignment can occur resulting in alterations to microsatellite repeats, often referred to as microsatellite instability (MSI). These defects in DNA repair pathways have been linked to human carcinogenesis. Mutations in the MLH1 gene have been reported to be found in tumors with MSI, such as some forms of colon cancer e.g., Hereditary nonpolyposis colon cancer (HNPCC), a subset of sporadic carcinomas and breast cancer.




Human colon stained with Anti-MLH1 using DAB chroogen

P

AM703-10M AY703-YCD


Clone: ES05

Isotype: IgG1

Source: Mouse

Immunogen: MLH1

Specificity: MLH1

Localization: Nuclei



Xmatrx: HX031-YCDE


MMP-9

Type IV collagenase (MMP-9), a 92-kD member of matrix metalloproteinase, is believed to play a critical role in the physiological tissue-remodeling processes and also in many pathological conditions such as tumor invasion. The induction of proteolytic enzymes is an important mechanism in the migration of monocytes into tissues and body fluids. MMP-9 is secreted from neutrophils, macrophages, and a number of transformed cells in zymogen form. MMP-9/gelatinase B is upregulated in angiogenic dysplasias and invasive cancers of the epidermis in a mouse model of multi-stage tumorigenesis elicited by HPV16 oncogenes. MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells.


AN504-10M



Lymphoma tissue stained with Anti-MMP-9 using DAB chromogen

Clone: EP1255Y

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues in the C-terminus of Human MMP-9.

Specificity: MMP-9




Xmatrx: HX031-YCDE

P


MSH2

MutS homologue 2 (MSH2) is a DNA mismatch repair protein that belongs to the MutS family. MSH2 forms two different heterodimers: MutS alpha (MSH2-MSH6) and MutS beta (MSH2-MSH3), which bind to DNA mismatches thereby initiating DNA repair. MSH2 is involved in DNA repair as a mismatch repair protein, and mutations of MSH2 are found in approximately 50% of inherited nonpolyposis colorectal carcinoma (HNPCC) (Lynch syndrome) cases.

Colon Ca. stained with anti-MSH2 using DAB chromogen

Clone: SP46

Isotype: IgG

Source: Rabbit

Immunogen: Synthetic peptide corresponding to internal region of human MSH2

Specificity: Human MSH2




Xmatrx: NoneReady-to-use (Manual): AN743-5M

AN743-10M


P


Primary Antibodies


MSH6

Mouse anti-MSH6 is a monoclonal antibody specific for MSH6. Inherited (germline) mutations in DNA mismatch repair genes such as MLH1, MSH2, MSH3, and MSH6 are the major causes of hereditary nonpolyposis colorectal cancer (HNPCC) syndrome. A characteristic of HNPCC tumors is microsatellite instability (MSI). Detection of microsatellite instability in a tumor sample will increase the probability of detecting a germline mutation in a DNA mismatch repair gene from the patient sample. Thus, MSI analysis is usually performed prior to proceeding with full mutation analysis of mismatch repair genes.


Concentrated:


AM454-5M

AM454-10M

MU454-UC

FG-454M

Clone: 2D4B5

Isotype: IgG3

Source: Mouse

Immunogen: Human MSH6

Specificity: MSH 6




Xmatrx: HX032-YCDEColon carcinoma stained with Anti-MSH6 using DAB chromogen

P


MUC4

MUC4 is a membrane-associated protein of the mucin (MUC) gene family, encoded by a gene on chromosome 3q29 and produced by epithelial cells as a heterodimer. The MUC4 protein is thought to play a protective role for vulnerable epithelia, particularly in the airway, eye, female reproductive tract, and mammary gland. Alterations in MUC4 expression have been observed in association with a variety of inflammatory and neoplastic states; reduction or loss has been reported in non-small cell lung carcinoma, hyperplastic polyps of the colon, and serrated colon adenomas, while overexpression of the MUC4/Sialomucin complex (SMC) has been identified in malignant progression of mammary tumors in humans.

Colonic mucosa stained with MUC4 antibody showing diffuse cytoplasmic positivity. (DAB chromogen used)

Clone: 1G8

Isotype: IgG1

Source: Mouse

Immunogen: Human MUC4

Specificity: MUC4






Concentrated:


AM455-5M

AM455-10M

MU455-UC

FG-455M

P


MUC5AC

Mucins are high molecular weight glycoproteins with 80% carbohydrates and 20% core protein. Gastric Mucin 5AC antigen is found in columnar mucus cells of surface gastric epithelium and in goblet cells of the fetal and precancerous colon but not in normal colon. Resurgence of gastric mucin during colonic carcinogenesis is suggestive of either re-expression of the peptide core of gastric mucin in the adult colon or due to changes in the glycosylation pattern of mucin, which expose the hidden Mucin 5AC antigen.

Gastro-intestinal tissue stained with Anti-MUC5AC using DAB chromogen

Clone: 45M1

Isotype: IgG1

Source: Mouse

Immunogen: Human MUC5AC

Specificity: MUC5AC




Xmatrx: HX031-YCDE


Concentrated:


AM456-5M

MU456-UC

FG-456M

P


Mucin 1 (MUC1)

Mucin 1 (MUC1) is a high molecular weight and heavily glycosylated transmembrane protein. MUC1 is normally expressed in secretory epithelial cells and functions to provide a protective layer and mediate cell-cell interactions. MUC1 is a prominent oncogene and is present in many carcinomas with abnormal expression levels, modified glycosylation, and aberrant intracellular localization. Such carcinomas include breast, colorectal, prostate, and pancreatic cancer.


AN564-10M


Breast carcinoma stained with Anti-Mucin-1 using DAB chromogen

Clone: EPR1023

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues on the C-terminus in human MUC1

Specificity: Mucin 1




Xmatrx: HX031-YCDE


P


113

Primary Antibodies


Mucin 2 (MUC2)

Mucins are a group of high molecular weight, highly glycosylated proteins expressed in normal and carcinogenic colon. MUC2 is a 520-kD glycoprotein of the gastrointestinal tract. The core of the glycoprotein consists of a variable number of tandem repeats of a 23 amino acid sequence. Mucin 2 is found in normal epithelial cells of the colon or in colon carcinoma. MUC2 glycoprotein is expressed in mucinous tumors but not in serous tumors. This antibody stains positive for colon gastric cancer cells, normal intestine, colon and salivary glands, and some human colon carcinoma cell lines (LS174T). This antibody localizes Mucin 2 (MUC2) protein in cytoplasm.



Clone: CCP58

Isotype: IgG1

Source: Mouse

Immunogen: Synthetic human MUC2 (MI-29) peptide (VNTR region)

Specificity: MUC2



Manual HK058-5K

Xmatrx: HX031-YCDE

Colon stained with Anti-Mucin 2 using DAB chromogen

P


Multi-Drug Resistance Marker (P-Glycoprotein)

Multi-Drug Resistance Marker (P-Glycoprotein) is a 170 kD cell membrane protein of the multi-drug resistance gene, MDR-1. Studies have linked the presence of P-Glycoprotein with resistance to a wide variety of chemotherapeutic agents. P-Glycoprotein is associated with an afflux pump that actively removes drug from the cell, thereby conferring resistance to a variety of drugs. P-Glycoprotein is also found in various concentrations in most normal tissues, suggesting that the primary role for this protein is in normal secretion of physiological metabolites. This antibody stains P-Glycoprotein in membrane and certain degree of cytoplasm of positive cells.

Adrenal gland tissue stained with Anti-multi-drug resistance marker using AEC chromogen

Clone: MDR88

Isotype: IgG1 Kappa

Source: Mouse

Immunogen: Recombinant P-glycoprotein containing four tandem repeats of the amino acid sequence 1096 through 1252, once of the cytoplasmic domains near the C-terminus

Specificity: Multi-Drug Resistance Marker


Pre-treatment: AR Citra/EZ-AR 1/EZ-AR 2

Manual: HK087-5KE

Xmatrx: HX031-YCD/EHX032-YCDE

P




Muscle Actins

Actin is a major component of the cytoskeleton and is present in every cell type. It is a globular protein, about 5 nm in diameter, composed of one polypeptide chain with a mass of approximately 47 kD. Four muscle actins have been identified: skeletal alpha, cardiac alpha, vascular smooth muscle alpha, and enteric smooth muscle gamma actin. These actins are very similar in their primary structure. Monoclonal Actin 88 is for the specific localization of actins in muscle tissue. Staining with this antibody distinguishes smooth muscle cells from fibroblasts in mixed cultures. This antibody stains skeletal, cardiac and smooth muscle cells.

Clone: Actin 88 Cocktail

Isotype: IgG

Source: Mouse

Immunogen: Synthetic peptides of actin

Specificity: Muscle actins


Pre-treatment: EZ-AR1

Xmatrx: HX031-YCDEMuscle tissue stained with Anti-Actin, Muscle Specific, using DAB chromogen

P




Myelin Basic Protein

Myelin Basic Protein (MBP), a single-chain, flexible polypeptide of about 18.5 kD is localized in both the compact myelin sheath and myelin ovoids. MBP has not been demonstrated in rough endoplasmic reticulum, lysosomes, or any other cytoplasmic organelles. MBP can be used as a marker for oligodendrocytes, Schwann cells and malignant Schwannomas. This antibody is useful in defining some of the elements in the catabolism of myelin in multiple sclerosis, experimental encephalomyelitis, and other diseases of the central nervous system. This antibody stains Myelin Basic Protein.


Cerebellum tissue stained with Anti-Myelin basic protein using AEC chromogen

Clone: MBP88

Isotype: IgG1

Source: Mouse

Immunogen: This antibody is the fusion product of SP/2 myeloma cells and the splenocytes from an A/J mouse immunized with peptide of Myelin Basic Protein

Specificity: Myelin Basic Protein



Manual/i6000: None

Xmatrx: HX031-YCDE

P


Primary Antibodies


Myeloid Specific Antigen

BM-3 is an early marker of myeloid differentiation. BM-3 recognizes a 13 kD myeloid specific antigen. The BM-3 antibody along with BM-1 and BM-2, provides the capacity to stain early precursor and mature forms of human myeloid cells. It is expressed during the early phases of myeloid differentiation. This antigen is present in human granulocytes, monocytes, and myeloid precursor cells. It has no reactivity with any other cell type in human tissues. This antibody stains cytoplasm in human granulocytes (98%) and monocytes (80%) residing in lymphoid and non-lymphoid tissues in formalin-fixed, paraffin-embedded tissue sections, bone marrow smears or blood smears.


Bone marrow stained with Anti-Myeloid Specific Antigen using Fast Red chromogen

Clone: BM-3

Isotype: IgG1

Source: Mouse

Immunogen: Nuclei from pokeweed mitogen stimulated human peripheral blood lymphocytes

Specificity: Myeloid Specific Antigen



Manual: HK058-5K

Xmatrx: HX031-YCDE

P


Myeloid Specific Antigen

This 183 kD myeloid specific antigen is a DNA binding protein expressed in early precursor myeloid cells. Monoclonal antibodies BM-1 and BM-2 are useful in the identification of early precursor and mature forms of human myeloid cells, respectively. The antigens are also expressed in granulocytic sarcomas and myeloid leukemias, myeloid precursor cells of bone marrow, scattered cells in the peripheral cortex of the thymus, granulocytes, granulocytic sarcomas, acute myelogenous leukemia (AML), chronic myelogenous leukemias and myelomonocytic leukemias. This antibody stains myeloid specific antigen in the nucleus of myeloid precursor cells of bone marrow, scattered cells in the peripheral cortex of the thymus, granulocytes, and granulocytic sarcomas.




Lymph node stained with Anti-Myeloid Specific Antigen using AEC chromogen

Clone: BM-1

Isotype: IgG1

Source: Mouse

Immunogen: Nuclei from human peripheral blood mononuclear cells

Specificity: Myeloid Specific Antigen



Xmatrx: HX032-YCDE

P


Myeloperoxidase (MPO)

Myeloperoxidase is an important enzyme used by granulocytes during phagocytic lysis of foreign particles engulfed. In normal tissues and in a variety of myeloproliferative disorders, myeloid cells of both neutrophilic and eosinophilic types at all stages of maturation, exhibit strong cytoplasmic reactivity for MPO. Erythroid precursors, megakaryocytes, lymphoid cells, mast cells, and plasma cells are nonreactive. MPO is not observed in the neoplastic cells of a wide variety of epithelial tumors and sarcomas. MPO is useful in differentiating between myeloid and lymphoid leukemias.


AR496-10R



Spleen tissue stained with Anti-Myeloperoxidase using DAB chromogen

Clone: Polyclonal

Isotype: IgG

Source: Rabbit

Immunogen: Purified human granulocytic MPO

Specificity: Myloperoxidase


Pre-treatment: EZ-AR™ 1/EZ-AR™ 2


Xmatrx: HX031-YCDE

P


AM432-5M

AM432-10M

MU432-UC

FG-432M

Myf4

Myf4 belongs to the family of the muscle regulatory proteins Myf3, Myf4, Myf5, and Myf6 that share a highly conserved DNA binding and dimerization domain consisting of a cluster of basic amino acids and a potential helix-loop-helix structure. Myogenin (Myf4) expressed early in skeletal muscle differentiation is a sensitive and specific marker for rhabdomyosarcoma and is more specific than desmin and muscle-specific actin and more sensitive than myoglobin.


Concentrated:


Clone: LO26

Isotype: IgG1

Source: Mouse

Immunogen: Recombinant fusion protein corresponding to the Myf4 gene

Specificity: Myf4



Manual: HK522-XAK

Xmatrx: HX032-YCDE

Rhabdomyosarcoma stained with Anti-Myf4 using DAB chromogen

P


115

Primary Antibodies


Myoglobin

Myoglobin functions as a cellular oxygen storage mechanism with some contribution to oxygen transport into the cell. The molecular mass of human myoglobin is 17.8 kD. Myoglobin is present exclusively in striated muscle, with the single exception of chicken gizzard smooth muscle. It is a valuable tool used in distinguishing rhabdomyosarcomas from other soft tissue tumors. After muscle tissue damage such as crush injuries, burns, myocardial infarction and muscle diseases, increased levels of myoglobin are found in the blood and urine. This antibody stains positive in the cytoplasm of muscle cells.




Skeletal Muscle stained with Anti-Myoglobin using DAB chromogen

Clone: MG-1

Isotype: IgG1

Source: Mouse

Immunogen: Purified human skeletal muscle myoglobin

Specificity: Myoglobin



Xmatrx: HX031-YCDE

P


Myoglobin

Myoglobin functions as a cellular oxygen storage mechanism with some contribution to oxygen transport into the cell. The molecular mass of human myoglobin is 17.8 kD. Myoglobin is present exclusively in striated muscle, with the single exception of chicken gizzard smooth muscle. Since myoglobin is the only striated muscle-specific antigen, it is a valuable tool used in distinguishing rhabdomyosarcomas from other soft tissue tumors. After muscle tissue damage such as crush injuries, burns, myocardial infarction and muscle diseases, increased levels of myoglobin are found in the blood and urine. This antibody reacts with human myoglobin.




Skeletal muscle stained with Anti-Myoglobin using AEC chromogen

Clone: Polyclonal

Source: Rabbit

Immunogen: Highly purified human myoglobin

Specificity: Myoglobin



Xmatrx: HX031-YCDE

P


AM331-10M

Myosin Heavy Chains, Smooth Muscle

SMMS.1 is approximately 204 kD and is considered to be the marker for smooth muscle cell phenotypes. It has been designed for specific localization of both vascular and visceral smooth muscle. Monoclonal antibody to smooth muscle myosin heavy chains in combination with monoclonal antibodies to calponin and heavy caldesmon may be used to study the differences between benign, in-situ lesions and invasive carcinomas. Monoclonal antibody stains smooth muscle myosin heavy chains in vascular and visceral smooth muscle, myoepithelial cells in normal and benign human mammary gland and certain stromal myofibroblasts.



Clone: SMMS.1

Isotype: IgG1

Source: Mouse

Immunogen: Crude human uterus caldesmon

Specificity: Smooth muscle myosin heavy chains




Xmatrx: HX032-YCDE

Myoepithelial cells stained with Anti-Myosin heavy chains using AEC chromogen

P


Myosin, Skeletal Muscle

Myosin along with actin forms the fundamental contractile unit of muscle, the myofibril. It has a molecular mass of 500 kD and is comprised of two identical heavy chains (200 kD each) and four light chains (15-20 kD). Monoclonal antibody MY-32 to fast-twitch skeletal myosin may be used for detecting cross-striated muscle differentiation in tumors. This antibody does not stain human or animal cardiac or smooth-muscle myosin. Staining of fast-twitch (type II) isomyosin molecules has been demonstrated on human skeletal muscle. The antibody stains human, rabbit, rat, mouse, bovine, chicken, and guinea pig skeletal myosin.




Skeletal muscle stained with Anti-Myosin using DAB chromogen

Clone: MY-32

Isotype: IgG1

Source: Mouse

Immunogen: Rabbit muscle myosin

Specificity: Skeletal-muscle myosin




P


Primary Antibodies


Napsin A

Napsin A has specific funtion in normal alveolar epithelim and is proposed to play a role in the protelytic processing of surfactant precursors. Napsin A is reported to be predominantly expressed in lamellar bodies of type II pneumocutes, secondary lysosymes of alveolar macrophages, respiratory epithelium of terminal and respiratory bronchioles, plasma cells within a subset of lymphocytes in normal lung, as well as in epithellial cells of renal tubiles in normal kidney and is weakly expressed in normal spleen.





Lung adenocarcinoma stained with anti-Napsin A using DAB chromogen

P

AM701-10M AY701-YCD


Clone: IP64

Isotype: IgG2b

Source: Mouse

Immunogen: Napsin

Specificity: Napsin A




Xmatrx: HX031-YCDE

N-Cadherin

N-Cadherin (Cadherin 2) is a calcium-dependent transmembrane protein involved in cell-cell adhesion. N-cadherin functions during gastrulation to regulate mesenchymal morphogenesis and angiogenesis. N-Cadherin has been noted for its role in cancer progression, particularly tumor metastasis. In the progression of a tumor, metastasis is often facilitated by the upregulation of N-Cadherin. The aberrant expression of N-Cadherin allows transendothelial migration which is needed for metastasis to occur while the lack of N-Cadherin will inhibit it.


Concentrated:

Colon carcinoma stained with Anti-N-Cadherin using DAB as chromogen

Clone: EPR1792Y

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues on the C-terminus of human Cadherin-2.

Specificity: N-Cadherin


Pre-treatment: AR Citra Plus /EZ-AR 1/ EZ-AR 2




P

AN565-5M

AN565-10M

NU565-UC

FG-565N


Neurofilament

Neurofilaments (10 nm diameter) and microtubules (25 nm diameter) comprise the main structural elements of neuronal axons, dendrites, and perikerya. Neurofilaments are composed of three major polypeptides referred to as the neurofilament triplet with approximate molecular weights of 200 kD, 160 kD and 68 kD. This antibody can be used for positive identification of neurons in the central and peripheral nervous systems. In general, co-expression of keratin and neurofilament should be interpreted as indicating neuroendocrine differentiation of a given tissue or neoplasm. The antibody stains Neurofilament in sections of brain and other tissues.


AM073-10MAX073-YCD




Nerve stained with Anti-Neurofilament using AEC chromogen

Clone: NE-14

Isotype: IgG1

Source: Mouse

Immunogen: Neurofilament purified from human brain

Specificity: Neurofilaments


Pre-treatment: Npne

P


Neuron Specific Enolase (NSE)

NSE is a gene which encodes for a protein found in matured neurons and is used in panels along with chromogranin, synaptophysin and neurofilament. Elevated NSE concentrations are observed in patients with neuroblastoma, pancreatic islet cell carcinoma, medullary thyroid carcinoma, pheochromocytoma, and other neuroendocrine tumors as well as certain benign conditions. NSE is specific for such proteins, and aids in detection of neural and neuroendocrine lineage.


AM055-10M




Pancreatic islets stained with anti-NSE using DAB chromogen

Clone: MIG-N3

Isotype: IgG1 Kappa

Source: Mouse

Immunogen: Purified human gamma enolase

Specificity: Neuron specific enolase (NSE)


Pre-treatment: None

P


117

Primary Antibodies


NGF Receptor

Neurotrophins signal cell survival, differentiation, growth cessation, and apoptosis through two classes of cell surface receptors, the Trks and NGF Receptor (p75NTR). p75NTR, when activated in the absence of a strong Trk signal, induces apoptosis of neurons, while in the presence of Trk it enhances responses to neurotrophin. p75NTR, when it is not bound by NGF, may play a role in neuronal selection during embryonic development and suggest that neuroblastomas may arise from immature neuroblasts that escape programmed cell death. Therefore, the loss of p75NTR expression in developing neural crest cells might be a primary event in the genesis of neuroblastoma.


AN522-10M


Neuroblastoma stained with Anti-NGF Receptor using DAB chromogen

Clone: EP1039Y

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues of human NGFR

Specificity: NGF Receptor




Xmatrx: HX031-YCDE

P


NGF Receptor

NGFR, also known as p75NTR, is a receptor of neurotrophins and involved in survival, differentiation and apoptosis of neurons. It is expressed in neuronal cells in various tissues and tumors with neuronal origin. NGFR is also expressed in melanocytes, myoepithelial cells, basal-like cells, perivascular cells and lymphoid dendritic cells. NGFR is helpful in identification of perineural invasion of malignant skin tumors with a panel of antibodies. It is also a complementary marker to S-100 for identification of desmoplastic melanomas.

Brain stained with anti-NGFR using DAB chromogen

Clone: EP31

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues of human NGFR protein

Specificity: NGFR




Xmatrx HX031-YCDE


AN738-10M



P



Nuclear Ribonucleoprotein

Monoclonal antibody 58-15 is one such antibody that recognizes a 36 kD nuclear antigen that is organized into discrete 20-80 nm electron-dense nuclear granules. Immunohistochemical analyses in a variety of different tumors indicate that antinuclear monoclonal antibodies may be useful for probing the cell cycle dependent modulation of nuclear antigens. This antibody stains a 20-80 nm electron dense nuclear structure with highest labeling densities found in nuclear ribonucleoprotein particles, although heterochromatin, euchromatin, and nucleoli may also be stained.



Spleen tissue stained with Anti-Nuclear ribonucleoprotein using DAB chromogen

Clone: 58-15

Isotype: IgG

Source: Mouse

Immunogen: Isolated nuclei

Specificity: Nuclear Ribonucleo protein particles



Manual: EK000-5K

P

Oct-2

Oct-2 is a transcription factor belonging to the POU homeo-domain family and is a major regulator found in B lymphocytes. By binding to the octamer motif, Oct-2 promotes immunoglobulin transcription and regulates various lymphoid-specific genes. All tumor cell lines from the B-cell lineage, and a few from the T-cell lineage, express Oct-2. Oct-2 expression is significantly greater in diffuse large B-cell lymphoma and lymphocyte predominant Hodgkin disease; therefore Oct-2 is a useful differential marker for these diseases.


AN558-10M



Tonsil stained with Anti-OCT-2 using DAB chromogen

Clone: EPR542

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues in human Oct.-2

Specificity: Oct-2




Xmatrx: HX032-YCDE

FG-558N

P


Primary Antibodies


Oct-4

Oct-4 transcription factor is initially active as a maternal factor in the oocyte but remains active in embryos throughout the preimplantation period. Oct-4 expression is associated with an undifferentiated phenotype and tumors. Oct-4 is a sensitive and specific marker for germ cell tumors. It is consistently detected in carcinoma in situ/gonadoblastoma, seminomas, germinoma, dysgerminoma, and embryonal carcinoma but not in the differentiated components of nonseminomas, i.e., teratomas, yolk sac tumors, and choriocarcinomas. It is useful in the identification of primary as well as metastatic germ cell tumors.

Testis stained with anti-Oct-4 using DAB chromogen

Clone: EP143

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues of human Oct-4 protein

Specificity: Oct-4




Xmatrx: HX032-YCDE


AN724-10M



P



Osteonectin

Bone matrix consists of collagen and non-collagenous proteins. Osteonectin, a 32-kD calcium-binding glycoprotein, is found in a variety of cell types, which include osteoblastic epithelial cells and fibroblasts. Osteonectin is a useful biochemical marker for bone-related tumors. Thus, osteonectin antibody can be used to demonstrate the presence of osteonectin in active osteoblasts and osteoprogenitor cells as well as in young osteocytes.

Clone: OST1

Isotype: IgG1

Source: Mouse

Immunogen: Human osteonectin

Specificity: Osteonectin protein




Xmatrx HX031-YCDEOsteosarcoma stained with Anti-Osteonectin using DAB chromogen

P

MU387-UC


Concentrated:


p105 Proliferation-Associated Nuclear Antigen

Antibody to p105 is directed against two polypeptides with molecular mass of 105 and 41 kD. Anti-p105 is the only immunological reagent known to preferentially stain interchromatin granules, a domain within the nuclear matrix responsible for RNA synthesis. The p105 antigen is thought to play an important role in RNA metabolism, RNA transport, or cell-cycle regulation. Like Ki-67, p105 is a proliferation-associated nuclear antigen that shows increased expression in proliferating cells. In normal cells p105 staining is absent during the early phases of the cell cycle. During G2 and mitosis, p105 levels increase dramatically. Antibody to p105 may prove useful for identifying malignancies and in studying chromatin structure and malignant transformations.



Clone: 2B3

Isotype: IgM

Source: Mouse

Immunogen: Pokeweed mitogen-stimulated human peripheral blood lymphocytes

Specificity: p105 proliferation-associated nuclear antigen



Manual: EK000-5K

Xmatrx: HX032-YCDE

Oral mucosa stained with Anti-p105 using AEC chromogen

P


p16 (INK4a)

p16(INK4a) is a tumor-suppressor protein and that genetic and epigenetic abnormalities in genes controlling the G1 checkpoint can lead to both escape from senescence and cancer formation. The interaction of p16(INK4a) family members can be a binary complex with CDK4/6 or ternary complex with cyclin D-bound CDK4/6 and ultimately results in the inhibition of cell cycle progression. As such, expression of p16(INK4a) is commonly associated with cellular senescence, and disruption of the p16(INK4a) gene is frequently observed in human tumors. The p16(INK4a) locus is deleted in a wide spectrum of tumors including melanoma, pancreatic adenocarcinoma, glioblastoma, certain leukemias and non-small cell lung cancer. For research use only. Not for use in diagnostic procedures.


AM540-10M



Cervical carcinoma stained with Anti-p16 using DAB as chromogen

Clone: G175-405

Isotype: IgG

Source: Mouse

Immunogen: Human p16 (INK4a) fusion protein

Specificity: P16

Localization: Nucleus and/or Cytoplasm



P


RUO

119

Primary Antibodies


p21/WAF1

The p21/WAF1 protein is a p53 regulated gene product that has been shown to mediate cell cycle arrest. The growth arrest is due to several properties of this protein, namely cyclin dependent kinase inhibition, and maintenance of cell cycle arrest at G2 by blocking the interaction of Cdc25C with PCNA and inhibition of stress activated protein kinases. In breast cancer the p21/WAF1 expression is generally seen to be negative. This antibody stains the nucleus in cells that are arrested in G1 phase.

Clone: 4D10

Isotype: IgG1

Source: Mouse

Immunogen: Recombinant fusion protein corresponding to full length WAF1 molecule

Specificity: p21/WAF1 antigen



Manual: HK081-5KE

Xmatrx: HX032-YCDE

Skin stained with Anti-WAF-1using AEC chromogen

P


AX434-YCD




p27 (Kip1)

The p27 Kip1 protein, also known as cyclin-dependent kinase inhibitor 1b (CDKN1B) or Kip1, is a putative tumor suppressor gene, regulator of drug resistance in solid tumors, and promoter of apoptosis. It acts as a safeguard against inflammatory injury and it has a role in cell differentiation. The p27 Kip1 protein is expressed in all normal tissues. The level of its expression has been observed to decrease during tumor development and progression in many tumors, including oral squamous cell carcinoma and in thyroid, colon, breast, prostate, and superficial bladder carcinomas. Overexpression of p27 Kip1 has been observed in a subset of aggressive B cell lymphomas.


AX396-YCD


Breast tissue stained with Anti-p27/Kip1 using AEC chromogen

Clone: DCS72

Isotype: IgG1

Source: Mouse

Immunogen: Recombinant rodent p27/ Kip1 antigen

Specificity: p27 Kip1 antigen



Manual: HK081-5KE

Xmatrx: HX032-YCDE

P


p27 (Kip1)

p27(Kip1) is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27(Kip1) binds to and inhibits cyclinE-CDK2 complex, cyclinA-CDK2 and cyclinD1-CDK4. p27(Kip1) is regulated by phosphorylation on serine 10 (S10) and threonine 187 (T187). Phosphorylation by CDK2 on T187 results in ubiquitylation and degradation of p27(Kip1); while phosphorylation by hKIS on S10 signals the nuclear export to the cytoplasm.


AN492-10M


Breast carcinoma stained with Anti-p27 Using DAB chromogen

Clone: Y236

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues in the C-term of human p27(Kip1).

Specificity: p27




Xmatrx: HX032-YCDE

P



p34cdc2 (Cyclin Dependent Kinase)

p34cdc2 is a phosphoprotein with protein kinase activity that functions in the G2/M phase transition of the cell cycle. It is the catalytic subunit of the maturation-promoting factor (MPF) and forms a complex with both cyclin A and B in mammalian cells. Activated p34cdc2 kinase phosphorylates a variety of substrates leading to some specific events of mitosis including nuclear envelope break-down and chromosome condensation. It has also been implicated in lymphoid proliferation. This antibody stains p34cdc2 cyclin dependent kinase in nucleus and cytoplasm of proliferating cells and tumor cells and cross-reacts with skeletal muscle cells.

Clone: POH-1

Isotype: IgG2a

Source: Mouse

Immunogen: Recombinant human p34cdc2 fusion protein

Specificity: p34cdc2 cyclin dependent kinase


Pre-treatment: AR Citra /EZ-AR 1/EZ-AR 2

Manual: HK087-5KE


Tonsil tissue stained with Anti-p34cdc2 using DAB chromogen

P


Concentrated:


AM301-5M

MU301-UC

FG-301M


Primary Antibodies


P504S (AMACR)

AMACR (P504S) is an acronym for the protein alpha-methylacyl CoA racemase that helps to metabolize certain fatty acids within the body. AMACR has been recently described as prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. High expression of AMACR (P504S) protein is usually found in prostatic adenocarcinoma but not in benign prostatic tissue by immunohistochemical staining in paraffin-embedded tissues. It stains premalignant lesions of prostate: high grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia. Several studies have suggested that AMACR can be used as a prostate cancer biomarker. Using AMACR (P504S) as a positive marker along with basal cell staining (34 beta E12 or p63) as a negative marker could help to confirm the diagnosis of small focus of prostate carcinoma on needle biopsies.


Concentrated:

Clone: 13H4

Isotype: IgG

Source: Rabbit

Immunogen: Human AMACR polypeptide

Specificity: P504S




Xmatrx: HX031-YCDE

AN449-5ME

NU449-UCE

Prostate carcinoma stained with Anti-P504S antibodyusing DAB chromogen

P

AN449-10ME



P504S (AMACR)

AMACR has been recently described as a prostate cancer-specific gene that encodes a protein involved in the beta-oxidation of branched chain fatty acids. High expression of AMACR (P504S) protein is usually found in prostatic adenocarcinoma but not in benign prostatic tissue by immunohistochemical staining in paraffin-embedded tissues. It stains premalignant lesions of prostate: high grade prostatic intraepithelial neoplasia (PIN) and atypical adenomatous hyperplasia. Using AMACR (P504S) as a positive marker along with basal cell staining (34 beta E12 or p63) as a negative marker could help to confirm the diagnosis of small focus of prostate carcinoma on needle biopsies.


AN538-10M



Prostate carcinoma stained with Rabbit Anti- P504S / AMACR using DAB as chromogen

Clone: RBT-AMACR

Isotype: IgG

Source: Rabbit

Immunogen: Human P504S

Specificity: P504S/AMACR




Xmatrx: HX031-YCDE

FG-538N

P


p53

Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycles, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, and it functions as a tumor suppressor within a variety of tumors by either stimulating apoptosis or growth arrest in deference to cell type and physiological factors. p53 is overexpressed in over 50% of human cancers. Positive staining of p53 detected by immunohistochemistry has been observed in colon cancer, breast cancer, lung cancer, prostate cancer and ovary cancer.

Breast Ca. stained with anti-P53

Clone: EP9

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to N-terminal residues of human p53 protein

Specificity: Human p53 protein




Xmatrx: None


AN728-10M



P



p53 Protein

p53 is a tumor suppressor gene product identified in a wide variety of tumors. p53 protein is present in low concentration in normal cells, but elevated levels of mutant p53 have been found in many common cancers. Accumulation of mutant p53 detected by immunohistochemical staining has been reported in breast, lung, colon, stomach, bladder, and testis carcinomas, soft-tissue sarcomas, and melanomas. This antibody stains positive in nucleus of a variety of tumor cells.





Breast carcinoma stained with Anti-p53 using DAB chromogen

Clone: BP53-12-1

Isotype: IgG2a

Source: Mouse

Immunogen: Recombinant human wild-type p53 protein

Specificity: p53 protein




Xmatrx: HX032-YCDE

P

AM195-10MAX195-YCD


121

Primary Antibodies


p53 Protein

p53 is a tumor suppressor gene product identified in a wide variety of tumors. p53 protein is present in low concentration in normal cells, but elevated levels of mutant p53 have been found in many common cancers. Accumulation of mutant p53 detected by immunohistochemical staining has been reported in breast, lung, colon, stomach, bladder, and testis carcinomas, soft-tissue sarcomas, and melanomas. This antibody stains positive in nucleus of a variety of tumor cells.


AM239-10MAX239-YCD





Clone: DO7

Isotype: IgG2b

Source: Mouse

Immunogen: Recombinant wild-type p53 protein





Xmatrx: HX032-YCDE

P


p53 Protein

p53 is a tumor suppressor gene product identified in a wide variety of tumors. p53 protein is present in low concentrations in normal cells, but elevated levels of mutant p53 have been found in many common cancers. Accumulation of mutant p53 detected by immunohistochemical staining has been reported in breast, lung, colon, stomach, bladder, and testis carcinomas, soft-tissue sarcomas, and melanomas. This antibody stains both wild-type and mutant human p53 protein primarily in nucleus of positive cells.





Clone: 1801

Isotype: IgG1

Source: Mouse

Immunogen: Fusion proteins of human p53 with β-galactosidase





Xmatrx: HX032-YCDE

P


p63

This antibody will detect all isoforms of p63 since the epitope is within the DNA binding domain. The p63 protein is a member of the p53 family, which also includes p73. p63 protein is detected in proliferating cells of epithelium, cervix, urothelium and prostate.





Prostate tissue stained with Anti-p63 using AEC chromogen

Clone: 4A4

Isotype: IgG2a

Source: Mouse

Immunogen: Amino terminal fragment of the delta Np63 isoform

Specificity: p63



Manual/i6000TM: HK081-5K

Xmatrx: HX032-YCDE

P


PAP

Prostate acid phosphatase (PAP) is a 100 kD glycoprotein present in high concentration in the prostate gland and its secretions. PAP is measured clinically because its level often rises in the serum in cases of prostatic carcinoma.


AM532-10M



Prostate Carcinoma stained with Anti-PAP using DAB chromogen

Clone: A40010

Isotype: IgG1

Source: Mouse

Immunogen: PAP purified from seminal fluid

Specificity: PAP




Xmatrx: HX031-YCDE

P


Papillomavirus Type 16 (HPV-16)

Infection with certain types of HPV, the so-called “oncogenic HPVs” including 16, 18, 31, 33, and 35, has been associated as a major risk factor in the subsequent development of cervical cancer.

Clone: Cam Vir-1

Isotype: IgG 2a

Source: Mouse

Immunogen: Recombinant HPV-16 protein

Specificity: HPV16




Xmatrx: HX032-YCDE

HPV infected tissue stained with Anti-HPV 16 using DAB chromogen

P



AX362-YCDEReady-to-use (Automated): Xmatrx®

Primary Antibodies


PAX-5

The PAX-5 gene is a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. The PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. The PAX-5 gene encodes the B-cell lineage specific activator protein (BSAP) that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis, therefore, PAX-5 gene product may not only play an important role in B-cell differentiation, but also in neural development and spermatogenesis.

Clone: ZP007

Isotype: IgG1

Source: Mouse

Immunogen: Human PAX-5

Specificity: PAX-5 antigen




Xmatrx: HX032-YCDEB cell showing PAX-5 positivity in a reactive lymph node stained using DAB chromogen


Concentrated:


AM457-5M

AM457-10M

MU457-UC

FG-457M

P


Paxillin (N-Term)

Paxillin is a cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion). Paxillin plays a significant role in integrin signaling and tyrosine phosphorylation of paxillin is required for integrin-mediated cytoskeletal reorganization. Paxillin is phosphorylated by focal adhesion kinase (FAK) at Tyr118. It has been found that phosphorylation of paxillin at both Tyr118 and Tyr31 is essential for MM1 cancer cell migration.


AN521-10M



Breast carcinoma stained with Anti-Paxillin using DAB chromogen

Clone: Y113

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues near the N-terminus of human Paxillin

Specificity: Paxillin





P


PDCD4

Programmed cell death protein 4 (PDCD4) is a tumor suppressor that inhibits tumor promoter-induced neoplastic transformation. It down-regulates the expression of MAP4K1, thus inhibiting events important in driving invasion, namely, MAPK85 activation and consequent JUN-dependent transcription. PDCD4 inhibits the translation initiation factor eIF4A, an RNA helicase that catalyzes the unwinding of secondary structure at the 5’ untranslated region (5’UTR) of messenger RNAs (mRNAs). It is also believed that regulated degradation of PDCD4 in response to mitogens allows efficient protein synthesis and consequently cell growth.


AN524-10M



Breast carcinoma stained with Anti-PDCD4 using DAB chromogen

Clone: EPR3432

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues near the C- terminus of human PDCD4 .

Specificity: PDCD4

Localization: Cytoplasm/Nucleus




P


PDGF Receptor-β

Human platelet-derived growth factor (PDGF) receptor, is a receptor tyrosine kinase whose activity induces various signalling cascades leading to cell growth and proliferation. Two major isoforms exist, PDGF receptor-α and PDGF receptor-β. PDGF receptor-β can only bind two specific PDGF isoforms, PDGF-β and PDGF-Δ. Upon ligand binding, the receptor undergoes autophosphorylation, allowing binding and activation of cytoplasmic SH2 domain-containing signal transduction molecules such as PI kinase, Grb2, and Src, among others.

Clone: Y92

Isotype: IgG

Source: Rabbit

Immunogen: Human- PDGFR- Beta

Specificity: PDGFR- Beta




Xmatrx: HX031-YCDEGIST showing cytoplasmic PDGF expression stained using DAB chromogen


Concentrated:


AN463-5M

AN463-10M

NU463-UC

FG-463N

P


123

Primary Antibodies


PGP9.5

PGP9.5/UCH-L1 is a member of a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to generate the ubiquitin monomer. PGP9.5 is a component of the ubiquitin system, which has value as a marker for neurons and may be of particular use in the study of ubiquitinated cellular inclusions characteristic of several chronic human neurodegenerative diseases. A fundamental role in regulating various biological activities, the PGP9.5 gene encodes two opposing enzymatic activities that affect alpha-synuclein degradation and Parkinson’s disease susceptibility.

Small intestine stained with anti-PGP9.5

Clone: 3D9

Isotype: IgG2a kappa

Source: Mouse

Immunogen: PGP9.5 antibody was raised in mouse using recombinant human PGP9.5 (1-223aa) purified from E. coli as the immunogen

Specificity: Human PGP9.5


Pre-treatment: AR Citra Plus//EZ-AR 1


Xmatrx: HX031-YCDE


AM736-10ME


P




Placental Alkaline Phosphatase (PLAP)

Human Placental Alkaline Phosphatase (PLAP), a 60-70 kD oncofetal antigen, is a member of a family of membrane bound alkaline phosphatase enzymes and isoenzymes. PLAP and/or PLAP-like isoenzymes have been found to be expressed by malignant tumors of germ cell and non-germ cell origin. The antibody reacts with PLAP in syncytiotrophoblasts in placenta and also reacts with human germ cell tumors. This antibody stains positive in cytoplasmic membrane and cytoplasm of positive cells.





Placenta tissue stained with anti-PLAP using DAB chromogen

Clone: PL8-F6

Isotype: IgG

Source: Mouse

Immunogen: Purified human placental alkaline phosphatase

Specificity: Placental alkaline phosphatase


Pre-treatment: None

P

AM228-10MAX228-YCD


Placental Lactogen (hPL)

Human Placental Lactogen (hPL) can be demonstrated in human placental tissue and in the serum of pregnant women. Human placental lactogen has been identified in some breast carcinomas and in trophoblastic and nontrophoblastic tumors of the placenta, and has been used as a serum or tissue marker for trophoblastic and nontrophoblastic neoplasms. This antibody stains hPL in cytoplasm of trophoblast and other positive cells.

Placenta tissue stained with Anti-Hpl using DAB chromogen

Clone: Polyclonal

Source: Rabbit

Immunogen: Human placental lactogen purified from human urine

Specificity: Human Placental Lactogen (hPL)



Manual/i6000: None

Xmatrx: HX031-YCDE

P




AR040-10RReady-to-use (Automated): i6000TM Xmatrx®

AW040-YCD

Platelet-Derived Growth Factor (PDGF)

PDGF is one of the major factors activated in wound healing and revascularization and may play an important role as an endogenous promoter in epithelial tumor foundation. PDGF can lead to excessive production of extracellular matrix components including various collagens, proteoglycans, and laminin. The development of specific subsets of smooth muscle cells depends on PDGF. PDGF is one of the most potent activators of stromal cells. PDGFR is a prime candidate to mediate proliferation and migration responses of mesangial injury in glomerular disease. This monoclonal antibody stains PDGF in cytoplasm of positive cells.



Clone: PDGF88

Isotype: IgM

Source: Mouse

Immunogen: Synthetic peptide of PDGF-B conjugated to keyhole limpet hemocyanin

Specificity: PDGF-B



Manual: HK087-5KE

Xmatrx: HX031-YCDE

Squamous cell carcinoma stained with Anti-PDGF using AEC chromogen

P


Primary Antibodies


Platelet-Derived Growth Factor (PDGF)

PDGF is one of the major factors activated in wound healing and may play an important role as an endogenous promoter in epithelial tumor foundation. PDGF can lead to excessive production of extracellular matrix components including various collagens, proteoglycans, and laminin. PDGF is one of the most potent activators of stromal cells. Proliferation and migration are important responses of mesangial cell injury. PDGFR is a prime candidate to mediate these responses in glomerular disease. PDGF and PDGFR are upregulated in the mesangium during glomerular injury. The monoclonal antibody to PDGF-B has been studied for its potential clinical utility in wound healing and revascularization. This epitope-specific antibody stains PDGF in cytoplasm of positive cells.

Squamous cell carcinoma stained with Anti-PDGF using AEC chromogen

Clone: Polyclonal

Source: Rabbit

Immunogen: Synthetic peptide based on PDGF-B sequence that shares high homology with PDGF-A forms

Specificity: PDGF



Xmatrx: HX031-YCDE

P




PMS2

PMS2 (post meiotic segregation increased 2) is a DNA mismatch repair protein that forms a heterodimer with MLH1. Mutations in these genes are associated with hereditary nonpolyposis colon cancer (HNPCC). It is demonstrated that some of pancreatic, gastric, breast, ovarian and small cell lung cancers also display microsatellite instability, this may suggest that DNA mismatch repair is not only restricted to HNPCC tumors but is a common feature in tumor initiation or progression.

Colon carcinoma stained with Anti-PMS2 using DAB as chromogen

Clone: EPR3947

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues in human PMS2 .

Specificity: PMS2


Pre-treatment: AR Citra Plus /EZ-AR 2


Xmatrx: HX032-YCDE


AN577-10M



P


Progesterone Receptor

The human progesterone receptor (PR), is a ligand-activated transcription factor and is a member of the steroid receptor family. PR exists in human as two isoforms; PR-A (94 kD) which lacks the first 164 amino acids of PR-B and PR-B(114 kD).This anti-PR recognizes both PR-A and B. It labels epithelial cells of breast, ovary and endometrium. This antibody stains human progesterone receptor in tissue sections by immunohistochemical techniques



Clone: EP2

Isotype: IgG

Source: Rabbit

Immunogen: Purified human progesterone receptor protein

Specificity: Progesterone Receptor




Xmatrx: HX031-YCDE

P

Breast carcinoma stained with Anti-PR using DAB chromogen



AN711-10MEReady-to-use (Automated): i6000TM Xmatrx® AY711-YCDE

Progesterone Receptor


Concentrated:

The presence of progesterone receptor in human breast cancer has been proposed as a mechanism whereby tumor cells respond to estrogen, and its presence may therefore serve as a marker for enhanced hormone responsiveness. Historically, estrogen receptor-positive/progesterone receptor-positive breast carcinoma patients have demonstrated a better response to endocrine therapy than estrogen receptor-positive/ progesterone receptor-negative patients. The use of monoclonal antibodies to determine progesterone receptor status increases the predictive value of immunohistochemical analysis with respect to the response of human tumors to hormonal modulation. This antibody stains human nuclear progesterone receptor in tissue sections by immunohistochemical techniques.

Breast carcinoma stained with Anti-PR using DAB chromogen

Clone: 1A6

Isotype: IgG1

Source: Mouse

Immunogen: Synthetic peptide of progesterone receptor





Xmatrx: HX032-YCDE

AM172-2ME

MU172-UCE

P



125

Primary Antibodies


Progesterone Receptor (InSite® PR)

The use of monoclonal antibodies to determine Progesterone Receptor status increases the predictive value of immunohistochemical analysis with respect to the response of human tumors to hormonal modulation. Historically, estrogen receptor-positive/progesterone receptor-positive breast carcinoma patients have demonstrated a better response to endocrine therapy than estrogen receptor-positive/ progesterone receptor-negative patients. This antibody stains positive in nucleus of the receptor positive cells.





Progesterone Receptor on breast carcinoma showing strong nuclear positivity using AEC chromogen

Clone: PR88

Isotype: IgG1 Kappa

Source: Mouse

Immunogen: Purified human progesterone receptor protein





Xmatrx: HX032-YCDE

P

AM328-10ME Ready-to-use (Automated): i6000TM Xmatrx® AX328-YCDE

Prolactin



Malignant pituitary adenomas or pituitary carcinomas arise from and consist of adenohypophysial cells. They can produce various hormones such as ACTH, Growth hormone, TSH, FSH, LH and Prolactin. Tumors that do not consist of adenohypophysial cells neither produce nor contain pituitary hormone, and thus immuno-peroxidase techniques are helpful in distinguishing from those pituitary tumors that store various hormones in the cell cytoplasm.

Pituitary gland stained with Anti-Prolactin using DAB chromogen

Clone: ME.121

Isotype: IgG1

Source: Mouse

Immunogen: Human Prolactin

Specificity: Prolactin


Pre-treatment: None

P


AX031-YCD

Proliferating Cell Nuclear Antigen (PCNA)




PCNA, also known as cyclin, is a 36 kD nonhistone nuclear protein that plays a fundamental role in the initiation of cell proliferation. PCNA is a cell cycle-regulated protein that preferentially occurs in dividing cells and is undetectable or present in small amounts in resting cells. Immunoperoxidase staining for PCNA in benign tissues has revealed positive nuclear staining in normal colonic crypt epithelium, gastric glandular cells, germinal center cells of lymph node, basal cells of skin, and renal tubular epithelial cells. The monoclonal antibody to PCNA might be an acceptable alternative to Ki-67 labeling in routinely processed tissues. This antibody stains PCNA in the nucleus of proliferating cells.

PCNA positivity in Breast carcinoma stained using DAB chromogen

Clone: PC10

Isotype: IgG2a

Source: Mouse

Immunogen: Rat PCNA synthesized with the protein A expression vector pR1T2T

Specificity: PCNA




Xmatrx: HX032-YCDE

P

AM252-10MAX252-YCD



Prostate Specific Acid Phosphatase (PSAP)

Prostate specific acid phosphatase (PSAP) is a 100 kD glycoprotein present in high concentration in the prostate gland and its secretions. PSAP is measured clinically because its level often rises in the serum in cases of prostatic carcinoma. By immunohistochemical analysis PSAP has been found concentrated within the large secretory vacuoles of the supranuclear portion of the prostatic columnar epithelial cell. In hyperplastic prostates and in benign prostatic tissue adjacent to the prostatic carcinoma, PSAP activity is limited to the acinar or ductal columnar epithelial cells and adjacent luminal content. For research use only. Not for use in diagnostic procedures.


AM013-10MEAX013-YCDEMU013-UCEConcentrated:



Prostate tissue stained with Anti-PSAP using DAB chromogen

Clone: B01-94-21M-NA

Isotype: IgG1 Kappa

Source: Mouse

Immunogen: Partially purified prostate acid phosphatase from human seminal plasma

Specificity: Prostate Specific Acid Phosphatase (PSAP)


Pre-treatment: None

P


RUO

Primary Antibodies


Prostate Specific Antigen (PSA)

PSA is a glycoprotein with a molecular mass of 33-34 kD. Clone ErPr8 is directed against a 35 kD protein identical to PSA. PSA is biochemically and immunologically distinct from prostatic acid phosphatase. It is restricted to the cytoplasm of acinar and ductal epithelia of normal, benign or malignant prostate tissue. This antibody is useful for determining if an isolated metastasis is of prostatic origin. Since PSA is released by prostatic tumors, it is also a valuable serum marker of neoplasia. For research use only. Not for use in diagnostic procedures.



Prostate tissue stained with Anti-PSA using DAB chromogen

Clone: ErPr-8

Isotype: IgG1

Source: Mouse

Immunogen: Affinity purified prostate specific antigen

Specificity: Prostate specific antigen (PSA)


P

AM014-10MEAX014-YCDE


FB-014MRecommended Barrier Control: RUO

pS2 Estrogen Inducible Protein

This antibody specifically recognizes 6.5 kD human pS2 estrogen-regulated protein (6.5 kD). pS2 is specifically expressed and secreted by ER-mucosa cells of the normal stomach (antrum and body) of both female and male individuals. Primary breast tumors have been shown to express pS2 in ER+primary breast tumors. This antibody shows a predominantly cytoplasmic localization of pS2 protein.


AM190-10M




Breast carcinoma stained with Anti-pS2 estrogen inducible protein using Fast Red chromogen

Clone: PS2.1

Isotype: IgG1

Source: Mouse

Immunogen: Synthetic peptide of 31 amino acid residues from the C-terminus of human pS2 protein

Specificity: pS2 protein


Pre-treatment: Protease/EZ-AR 1

Manual/i6000 EK002-5K

Xmatrx: HX031-YCDE or EK002-5K

P


PSMA

Prostate-specific membrane antigen (PSMA), also known as folate hydrolase 1 (FOLH1), is a type II transmembrane glycoprotein belonging to the M28 peptidase family. PSMA has two enzymatic activities, one as a prostate-specific integral membrane folate hydrolase and the other as a carboxypeptidase. An antibody to PSMA lables normal prostate epithelial cells and prostate tumor cells. Although the expression of PSMA in neovasculature of a variety of solid tumors has been reported, it is a useful marker for prostate tumors. In prostate cancer, overexpression of PSMA is correlated with high tumor grade, non-diploid tumors and advanced tumor state. It can be used as an effective predictor for tumor progression in prostate cancer.

Prostate stained with anti-PSMA

Clone: EP192

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues of human PSMA protein

Specificity: PSMA protein




Xmatrx: HX032-YCDE


AN714-10M



P



RCC (Renal Cell Carcinoma)

Renal cell carcinoma, also known by a gurnistical tumor, is the most common form of kidney cancer arising from the renal tubule. RCC antibody recognizes a 200 kD glycoprotein localized in the brush border of the proximal renal tubule. It immunoreacts with approximately 90% of primary renal cell carcinomas and approximately 85% of metastatic renal cellcarcinomas. Other tumors that may react with this antibody are parathyroid adenoma, an occasional breast carcinoma. Nephroblastoma, oncocytoma, mesoblastic nephroma, transitional cell carcinoma, and angiomyolipoma are not labeled with this antibody

Renal Cell carcinoma stained with Anti-RCC using DAB chromogen

Clone: RCC-26

Isotype: IgG1/K

Source: Mouse

Immunogen: Human RCC

Specificity: Renal Glycoprotein




Xmatrx: None


AM543-10M



P


127

Primary Antibodies


S100-β

S100 belongs to the family of calcium binding proteins such as calmodulin and troponin C. S100 Beta is abundant in glial cells of the central and peripheral nervous system, in melanocytes, chondrocytes, and adipocytes. It also labels Langerhans cells, histiocytes, epithelial, myoepithelial cells and integrating reticular cells of lymphoid tissue, and tumors originated from these cells. S100 Beta is a useful marker for diagnosis of melanoma, tumors of nerves system.

Melanoma stained with anti-S100 beta antibody using DAB

Clone: EP32

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues on the C-terminus of human S100 Beta protein

Specificity: S100 Beta protein




Xmatrx: HX032-YCDE


AN713-10M



P



S100 Protein

Ready-to-Use (Manual): AR058-5R

PU058-UPConcentrated:FG-058PRecommended Positive Control:

Clone: Polyclonal

Source: Rabbit

Immunogen: S-100 protein isolated polyclonal from bovine brain using affinity chromatography

Specificity: S100 protein

Localization: Cytoplasm & Nucleus

Tissue Type: FFPE


Manual/i6000: None


Melanoma stained with anti- S100 using AEC chromogen

AR058-10RReady-to-use (Automated): i6000TM

Xmatrx® AW058-YCD

P

S100 protein is a low molecular weight soluble protein first isolated from the brain and initially believed to be exclusively a glial marker. Two subunits of S100 protein have been identified. and they are differently expressed by various cells. the beta subunit is present in all S100 positive cells and tumors. In contrast, the alpha subunit is detectable only in neurons and lymph node macrophages. The presence of S100 protein is readily demonstrated in routinely processed malignant melanomas. S100 protein also has been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, in addition to glial cells. Neoplasms derived from these cells also express S100 protein to varying degrees. A large proportion of well-differentiated tumors of salivary gland, adipose, cartilaginous tissue, and Schwann cell derived tumors express S100 protein.

S100 Protein

S100 protein is a low molecular weight soluble protein first isolated from the brain and initially believed to be exclusively a glial marker. Two subunits of S100 protein have been identified. The beta subunit is present in all S100 positive cells and tumors. In contrast, the alpha subunit is detectable only in neurons and lymph node macrophages. The presence of S100 protein is readily demonstrated in routinely processed malignant melanomas. S100 protein has also been found in normal melanocytes, Langerhans cells, histiocytes, chondrocytes, lipocytes, skeletal and cardiac muscle, Schwann cells, epithelial and myoepithelial cells of the breast, salivary and sweat glands, in addition to glial cells. Neoplasms derived from these cells also express S100 protein to varying degrees. A large proportion of well-differentiated tumors of salivary gland, adipose, cartilaginous tissue, and Schwann cell-derived tumors express S100 protein.


AM058-10MAX058-YCD

MU058-UCConcentrated:FG-058MRecommended Positive Control:


Melanoma stained with Anti-S100 using DAB chromogen

Clone: 15E2E2

Isotype: IgG 2a Kappa

Source: Mouse

Immunogen: Purified bovine S-100 protein

Specificity: S100 protein


Pre-treatment: None

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S100P

S100P is a member of the S100 family of proteins. S100P is expressed in various normal tissues including placenta, bladder, spleen, gastric and intestinal mucosa. Overexpression of S100P has been detected in several cancers such as colon, prostate, pancreatic and lung carcinomas. It has been functionally implicated in carcinogenic processes. S100P is an early developmental marker of pancreatic carcinogenesis and can be used as a marker for pancreatic ductal adenocarcinoma. It may also serve as a predictor of distant metastasis and poor survival in non-small cell lung carcinomas.

Lung stained with anti-S100p

Clone: EP186

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues of human S100P protein

Specificity: S100P protein

Localization: Cytoplasm/Nucleus



Xmatrx: None


AN712-10M



P



Primary Antibodies


Sarcomeric Actin

Actin is a cytoskeletal protein that regulates cell motility, secretion, phagocytosis and cytokinesis. The NH2-terminal of actin may function as an antigen. This terminal may also modulate actin interactions and may associate with proteins such as myosin. This antibody is specific for alpha isoform of skeletal and cardiac muscle actin. This antibody shows wide cross reactivity to other tissues from human, sheep, rabbit, guinea pig, rat, frog and snake. However, it does not react with smooth muscle tissue.


AM511-10M

MU511-UCConcentrated:FG-511MRecommended Positive Control:

Muscle tissue stained with Anti-Sarcomeric Actin using DAB as chromogen

Clone: ZMSA-5

Isotype: IgG

Source: Mouse

Immunogen: Mouse anti-sarcomeric actin antibody is purified from mouse ascites.

Specificity: Sarcomeric Actin




Xmatrx: HX031-YCDE

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Secretin

This hormone, a polypeptide of 27 amino acids, which acts to stimulate pancreatic bicarbonate, is localized primarily in the gastrointestinal tract. It is released from secretin cells (S-cells) which have been localized within the antropyloric, duodenal, jejunal and ileal mucosa of human tissue. Hypersecretinemia has been observed in duodenal ulcers, Zollinger-Ellison syndrome, and chronic renal failure. This antibody stains Secretin in cellular elements in the epithelium of the gastrointestinal tract.

Stomach tissue stained with Anti-Secretin stained with AEC chromogen

Clone: Polyclonal

Source: Rabbit

Immunogen: Synthetic porcine secretin coupled to keyhole limpet hemocyanin with carbodiimide; conjugate emulsified in Freund’s complete adjuvant prior to injection

Specificity: Secretin



Xmatrx: HX031-YCDE

P




Stat 5-Alpha (C-term)

Stat 5 is part of a 7 protein family known as signal transducer and activator of transcription (STAT) which contributes to signal transduction by cytokine, hormone and growth factor. Signal transducer and activator of transcription 5 (Stat 5) functions in signal translocated to the nucleus in response to phosphorylation. Stat 5-alpha tyrosine phosphorylation is activated predominantly by IL-2 but also by IL-3, IL-5, IL-7, IL-9, IL-15, G-CSF and GM-CSF. Tyrosine phosphorylation is required for maximal transcriptional activity. NCoA-1/SRC-1 acts as coactivator for both the alpha and beta isoforms of Stat 5.


AN480-10M



Breast carcinoma stained with Anti-Stat 5-alpha using DAB chromogen

Clone: E289

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues in C-terminus of human Stat-alpha

Specificity: Stat 5-Alpha (C-term)





P


Substance P

Substance P is one of several neuroendocrine polypeptides localized in both the nervous system and gastrointestinal tract. Substance P is grouped into a family with bombesin and neurotensin because all three peptides are located in both brain and gut and terminate with a common dipeptide sequence (-Leu-Met-NH 2 ) at the amino terminal end. Substance P is found in most mid-gut and about half of fore-gut and hind-gut intestinal carcinoids. This antibody cross-reacts with other species including chicken and opossum. This antibody stains Substance P in nerve fibers.





FG-069P

Brain tissue stained with Anti-Substance P using AEC chromogen

Clone: Polyclonal

Source: Rabbit

Immunogen: Synthetic Substance P bound to keyhole limpet hemocyanin (KLH)

Specificity: Substance P




P

129

Primary Antibodies


Survivin

Survivin is a unique member of the inhibitor of apoptosis (IAP) protein family that interferes with post-mitochondrial events including activation of caspases. Survivin regulates cell cycle and is expressed in most tumors, but it is barely detectable in the terminally differentiated normal cells and tissues. The differential expression of Survivin in cancer versus normal tissues makes it a useful tool in cancer diagnosis and a promising therapeutic target.


AN503-10M



Bladder carcinoma stained with anti-Survivin using DAB chromogen

Clone: EP2880Y

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues in the N-terminus of Human Survivin

Specificity: Survivin


Pre-treatment: EZ-AR™1/EZ-AR 2


Xmatrx: HX032-YCDE

P


Synaptophysin

Synaptophysin, a 38 kD glycoprotein, is the major integral membrane protein of synaptic vesicles. It is a sensitive quantitative molecular marker of synaptic density and also a useful marker in the identification and characterization of neuronal and neuroendocrine neoplasms of the adrenal medullary, pituitary, thyroid and islet cell tumors, gastrointestinal, bronchial, thymic and pancreatic carcinoid tumors. Immunohistochemistry of synaptophysin has been used in the evaluation of functional bowel disorders, cortical epileptogenesis, schizophrenia and amyotropic lateral sclerosis.




Cerebellum stained with Anti-Synaptophysin using AEC chromogen

Clone: Snp88

Isotype: IgG3 Kappa

Source: Mouse

Immunogen: Recombinant human synaptophysin

Specificity: Synaptophysin protein




Xmatrx: HX031-YCDE

P

AM363-10MAX363-YCD



Tau

Tau’s major role is to regulate neuronal microtubule assembly and stability thus playing a major role in movement disorders. Neurofibrillary tangles (NFTs), one of the histopathological signs of Alzheimer’s Disease, contain a lot of incorporated Tau protein. Anti-Tau antibody shows strong positive staining in NFT areas and may also stain pick bodies in Pick’s Disease. In normal tissue, the antibody may stain neurons and axons in the brain and spinal cord. This antibody stains the cytoplasm of neurons and its connected axon.





Clone: Tau-2

Isotype: IgG1

Source: Mouse

Immunogen: Purified bovine Microtubule Associated Protein Tau (MAPT)

Specificity: Tau protein




Xmatrx: HX031-YCDE

Cerebellum stained with Anti-Tau using DAB chromogen

P

AM412-10MAX412-YCD


Tau

This antibody recognizes proteins of 45-68 kD, identified as tau proteins. The Tau monoclonal antibody reacts with the non-phosphorylated as well as the phosphorylated forms of tau. Tau proteins are members of the microtubule associated proteins (MAPs) that stabilize neuronal microtubules in cell processes, establishment of cell polarity and intracellular transport. Six isoforms, ranging from 352 to 441 amino acids, are generated from a single Tau gene by alternative splicing in the human central nervous system. In Alzheimer’s disease, abnormally phosphorylated, tau proteins aggregate into paired helical filaments and loose their ability to maintain the microtubule tracks. Missense Tau mutations in individuals with a type of frontotemporal dementia, FTDP 17, have been discovered.

Tau expression in normal brain tissue stained using DAB chromogen

Clone: Tau-5

Isotype: IgG1

Source: Mouse

Immunogen: Human Tau

Specificity: Tau




Xmatrx: HX031-YCDE


Concentrated:


AM459-5M

AM459-10M

MU459-UC

FG-459M

P



Primary Antibodies


Terminal Deoxynucleotidyl Transferase (TdT)

This antibody identifies a 58 kD peptide normally found in cortical thymocytes and immature bone marrow lymphocytes. TdT expression has been reported to occur in a majority of cases of acute lymphocytic leukemia (ALL) cases. TdT staining is found in all subtypes of ALL with the exception of pre-B-cell ALL. TdT positivity has also been observed in approximately one third of all cases of chronic myeloid leukemia. TdT positive staining is found in ALL, acute myeloid leukemia and chronic myeloid leukemia. This antibody stains predominantly nuclear TdT in normal and neoplastic cells.



Clone: TdT88

Isotype: IgG

Source: Mouse

Immunogen: Peptide containing specific sequence for N-terminal of human TdT protein

Specificity: TdT




Xmatrx: HX031-YCDE

Thymoma stained with Anti-Terminal deoxynucleotidyl Transferase (TdT) using AEC chromogen

P


AX373-YCD

Terminal Deoxynucleotidyl Transferase (TdT)

Terminal deoxynucleotidyl transferase (TdT) is a nuclear protein widely used as a marker for evaluation and classification of acute leukemia. In the study of small round cell tumors, TdT is often used as a marker for lymphoblastic lymphomas and leukemias. TdT is widely used to stain apoptotic cells.



Thymoma stained with Anti-Terminal deoxynucleotidyl Transferase (TdT) using AEC chromogen

Clone: Polyclonal

Source: Mouse

Immunogen: Synthetic peptide

Specificity: TdT antigen




Xmatrx: HX032-YCDE

P

AR373-10RReady-to-use (Automated): i6000TM Xmatrx® AW373-YCD

TFPU.1

Transcription factor PU.1 is a member of the Ets family of transcription factors and is required for the development of multiple hematopoietic lineages. It plays a pivotal role in normal myeloid differentiation, and regulates the expression of immunoglobulin and other genes that are important for B-cell development. The function of PU.1 is down-regulated by AML1-ETO in myeloid leukemia, whereas overexpression of PU.1 restores normal differentiation. The lack of transcription factor PU.1 protein expression in classical Hodgkin’s disease (cHD), a lymphoproliferative disease of predominantly B-cell origin, likely contributes to the lack of immunoglobulin expression and incomplete B-cell phenotype characteristic of the Reed-Sternberg cells in cHD.


AN520-10M



Lymphoma stained with Anti-TFPU using DAB chromogen

Clone: EPR3159Y

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to residues on the C- terminus of transcription factor PU.1

Specificity: TFPU




Xmatrx: HX032-YCDE

P


Follicular adenoma stained with anti-Thyroglobulin using AEC chromogen

Thyroglobulin

Thyroglobulin is a 19S glycoprotein with a molecular mass of approximately 650 kD. It constitutes 85-100% of the total of all thyroid iodoproteins. Immunohistochemical studies of thyroid carcinomas have revealed that a high portion of differentiated thyroid carcinomas synthesize thyroglobulin. Positive thyroglobulin staining indicates thyroidal origin of the tumor. Immunohistochemical and electron microscopic findings have disclosed a wide range of cellular differentiation in thyroid adenomas.




AM032-5M

Clone: 2H11

Isotype: IgG1

Source: Mouse

Immunogen: Purified human thyroglobulin

Specificity: Thyroglobulin


Pre-treatment: None

P


AX032-YCD

131

Primary Antibodies


Thyroid Stimulating Hormone (TSH)

Thyrotrophs produce Thyroid Stimulating Hormone (TSH). TSH is a 28 kD glycoprotein that contains 201 amino acid residues and is composed of alpha and beta subunits. The alpha subunit (MW 13kD) is immunologically similar to the alpha subunit of the other anterior pituitary hormones. The beta subunit is unique to TSH and is responsible for the specific biological activity of TSH. To identify thyrotrophs without cross-reactivity with gonadotrophs, antibodies directed to the TSH beta subunit must be used. This antibody stains TSH and b-TSH in cytoplasm of postive cells.



Pituitary cell showing cytoplasmic positivity for TSH stained using DAB chromogen

Clone: 5404

Isotype: IgG1 Kappa

Source: Mouse

Immunogen: TSH

Specificity: Thyroid Stimulating Hormone (TSH)


Pre-treatment: None

P


AX033-YCD

Thyroid Stimulating Hormone (TSH)

Thyrotrophs produce Thyroid Stimulating Hormone (TSH). TSH is a 28 kD glycoprotein that contains 201 amino acid residues and is composed of alpha and beta subunits. The alpha subunit (MW 13kD) is immunologically similar to the alpha subunit of the other anterior pituitary hormones. The beta subunit is unique to TSH and is responsible for the specific biological activity of TSH. To identify thyrotrophs without cross-reactivity with gonadotrophs, antibodies directed to the TSH beta subunit must be used. This antibody stains positive for TSH in cytoplasm of thyrotrophs.


Pituitary cell showing cytoplasmic positivity for TSH stained using AEC chromogen

Clone: Polyclonal

Source: Rabbit

Immunogen: Purified TSH from human pituitary gland

Specificity: Thyroid Stimulating Hormone (TSH)



Xmatrx: HX031-YCDE

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Thyroid Transcription Factor-1 (TTF-1)

Thyroid Transcription Factor-1 (TTF-1) is a 40 kD protein that is a member of NKx2 family of homeodomain transcription factors that regulates the expression of thyroid- and lung-specific genes. It is a very selective marker for adeno carcinomas of lung and thyroid origin. Nuclear localization of this protein is seen in the epithelial cells of thyroid gland and lung. The anti-TTF-1 antibody is a useful tool for differentiating pulmonary adeno carcinoma from metastatic breast carcinoma and mesothelioma. This antibody stains TTF-1 antigen located in the nucleus of thyroid and lung normal and carcinoma cells and other positive cells.




Clone: 8G7G3/1

Isotype: IgG1 Kappa

Source: Mouse

Immunogen: Recombinant TTF-1 protein

Specificity: TTF-1 antigen




Xmatrx: HX031-YCDEThyroid carcinoma cells expressing nuclear positivity for TTF1 stained using DAB chromogen

P

AM397-10MAX397-YCD


Thyroxine

The main hormones produced by the thyroid are Thyroxine (T4 or tetraiodothyronine) and, on a much smaller scale, triiodothyronine (T3). T4 and T3 have been demonstrated in normal and neoplastic thyroid follicular cells. In thyroid cancer, however, the iodine content may be 1/100 that of normal thyroid tissue, whereas thyroglobulin is much more abundant, occurring at 1/2 to 1/3 that of a normal thyroid. This antibody stains colloid in thyroid follicle and cytoplasm of thyroid follicular cells.

Thryroid tissue stained with Anti-Thyroxine using AEC chromogen

Clone: D5

Isotype: IgG1

Source: Mouse

Immunogen: Me-Thyroxine conjugated to bovine serum albumin

Specificity: Thyroxine (T4)




P

Ready-to-use (Manual): AM034A-5M




Primary Antibodies


TIA-1

The T cell intracellular antigen 1 (TIA-1) is a 17-kD cytoplasmic granule associated protein also designated as GMP-17, for granule membrane protein of 17 kD. The GMP-17/TIA-1 molecule is expressed in cells possessing cytolytic potential and could be involved in the signaling cascade of Fas (CD95)-mediated apoptosis. Within hematopoietic cell lines, the 2G9 monoclonal antibody (mAb) reacts with about 90% of CD16+, 50 – 60% of CD8+, and less than 10% of CD4+ normal peripheral blood lymphocytes. It reacts with almost all monocytes and granulocytes. This antibody also reacts with CD4+ activated T-cell clones, activated NK cell clones, and Con A activated thymocytes, but not with B lymphocytes or B-cell lines.


AM529-10M


Anaplastic large T Cell Lymphoma stained with Anti-TIA using DAB chromogen

Clone: 2G9A10F5

Isotype: IgG

Source: Mouse

Immunogen: Human bone marrow malignant cells from a non-B, non-T acute leukemia

Specificity: TIA-1




Xmatrx: HX031-YCDE

P


Topo 2 Alpha

Topoisomerase IIa (Topo 2α) is a ubiquitously expressed enzyme that regulates DNA topology in a cell. Because of its ability to unwind the DNA helicase, Topo 2α is an enzyme essential for DNA replication and protein synthesis. It has been linked to cell proliferation and mitotic processes. Topo 2α overexpression has been linked to a number of human malignancies. Topo 2α has been further identified as a useful marker in evaluating breast and thyroid malignant tumors.


AN576-10M



Breast tissue stained with Anti-Top 2 Alpha using DAB as chromogen

Clone: EP1102Y

Isotype: IgG

Source: Rabbit

Immunogen: A synthetic peptide corresponding to C-terminal residues of human Topo 2 Alpha

Specificity: Topo 2 Alpha




Xmatrx: HX032-YCDE

FG-576N

P


Toxoplasma gondii

Toxoplasma gondii is a common protozoan parasite of humans and vertebrate animals. In human hosts with a competent immune system, T. gondii infection generally develops into an asymptomatic chronic infection, with the organism sequestered in dormant “tissue cysts” often for the lifetime of the host. However, in immunocompromised hosts, such as AIDS patients or organ transplant recipients receiving immunosuppressive therapy, infection can lead to toxoplasmosis. The disease is characterized by the proliferation of T. gondii tachyzoites, which can damage the central nervous system, and can be fatal if untreated. During acute infection, T. gondii tachyzoites may be found in the bloodstream (but not within RBCs), in tissue, or within various cells, especially reticuloendothelial cells, leukocytes, and epithelial cells. This antibody stains Toxoplasma gondii in the cytoplasm of infected cells or tissues stained by immunohistochemical techniques. For research use only. Not for use in diagnostic procedures.

Infected cells stained with Anti-Toxoplasma using DAB chromogen

Clone: Polyclonal

Source: Rabbit

Immunogen: This antibody was produced by immunization of rabbits with live organisms of Toxoplasma gondii strain C56.

Specificity: Toxoplasma gondii

Localization: T. gondii

P



RUORecommended Positive Control: FG-125P

Transferrin

Human transferrin, an iron-binding protein, is produced mainly in the liver, and can be demonstrated within hepatocytes. Transferrin has also been demonstrated by immunohistology in a wide variety of other tissues including stomach, duodenum, gall bladder, thyroid, kidney, male and female reproductive tracts, skin, and in histiocytes. Such widespread occurrence of transferrin suggests evidence for the diverse roles that it may play such as iron transport across intestinal mucosa, intracellular iron transport, and providing non-specific immunity against micro-organisms by chelating free iron.

Liver tissue stained with Anti-Transferrin using AEC chromogen

Clone: HT1/13.6.3

Isotype: IgG1

Source: Mouse

Immunogen: Transferrin

Specificity: Transferrin



Xmatrx: HX031-YCDE

P




133

Primary Antibodies


Transforming Growth Factor (TGF), Alpha

Transforming Growth Factor, alpha (TGF-α) is a 50 amino acid peptide that is involved in the regulation of normal and malignant cell growth. The mature peptide is released following proteolytic cleavage from a 160 amino acid transmembrane precursor molecule. It is one of the various ligands for EGFR and seem to be involved in the growth regulation of intestinal mucosa and might be related to the development and progression of gastrointestinal tumors. Macrophages secrete TGF-α to trigger proliferation of cancer cells. TGF-α is synthesized by several cells, like epidermal keratinocytes, fibroblasts, and cells of hematopoetic origin like eosinophils and simulated macrophages.



Breast carcinoma showing TGF positivity stained using AEC chromogen

Clone: TGF88

Isotype: IgG1

Source: Mouse

Immunogen: Synthetic peptide representing a unique epitope to pro-TGF-α covalently bound to keyhole limpet hemocyanin

Specificity: Transforming growth factor, alpha (TGF-α )



Xmatrx: HX031-YCDE

P


Tumor-Associated Glycoprotein (TAG-72)

Tumor-Associated Glycoprotein 72 (TAG-72) is an oncofetal mucin antigen expressed by normal secretory endometrium and most human adenocarcinomas, including colorectal, gastric, pancreatic, mammary, and ovarian. This antigen is expressed by invasive ductal breast carcinomas, colon, pancreatic, gastric, esophageal, lung, ovarian and endometrial adenocarcinomas. It is not expressed by leukemias, lymphomas, sarcomas, mesotheliomas, melanomas, or benign tumors. This antigen is also expressed on normal secretory endometrium, but not on other normal tissues. This antibody stains positive in the cytoplasm of specific carcinoma cells.


AM054-10MAX054-YCD


FB-054MRecommended Barrier Control:FG-054MRecommended Positive Control:

Breast carcinoma stained with Anti-TAG-72 (BCA) using DAB chromogen

Clone: B72.3

Isotype: IgG1

Source: Mouse

Immunogen: Membrane-enriched fraction of a breast carcinomaderived from a liver metastasis

Specificity: Tumor-Associated Glycoprotein (TAG-72)


Pre-treatment: None

P


Tumor-Associated Glycoprotein (TAG-90, BCA)

Clone B6.2 recognizes a 90 kD glycoprotein in mammary carcinomas, metastatic lymph nodes, lung carcinomas, and adenocarcinomas. This antibody reacts intensely with tumor cells, yet is unreactive with cells in normal tissue. This antibody reacts equally with breast cancer, breast fibroadenoma, lobular carcinoma of the breast, duct carcinoma of the breast, and lung carcinoma. It also reacts with gastric and papillary adenocarcinomas, and adenocarcinoma of the colon, ovary, pancreas, lung and prostate. This antibody stains positive in the cytoplasm of tumor cells.

Breast carcinoma stained with Anti-TAG-90 (BCA) using DAB chromogen

Clone: B6.2

Isotype: IgG1

Source: Mouse

Immunogen: Membrane-enriched fraction of breast tumor metastatic to the liver

Specificity: 90 kD tumor-associated glycoprotein



Manual: EK000-5K

Xmatrx: HX031-YCDE or EK000-5K

P





Tyrosinase

Tyrosinase is a copper-containing enzyme present in plant and animal tissues that catalyzes the production of melanin and other pigments from tyrosine by oxidation. The gene for tyrosinase is regulated by the microphthalmia-associated transcription factor. A mutation in the tyrosinase gene resulting in impaired tyrosinase production results in type I oculocutaneous albinism, a hereditary disease that one in every 17,000 person has in the US. Anti-tyrosinase has been found to be quite specific for melanotic lesions such as malignant melanoma, and melanotic neurofibroma. Essentially no carcinomas express this marker.


AM535-10M



Melanoma stained with Anti-Tyrosinase using DAB chromogen

Clone: Ty/G5

Isotype: IgG2a

Source: Mouse

Immunogen: Human Tyrosinase

Specificity: Tyrosinase




Xmatrx: HX031-YCDE

FG-535M

P


Primary Antibodies


VEGF

Vascular endothelial factors (VEGFs) are a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors. VEGF receptors stimulate the proliferation of endothelial cells, induce angiogenesis, and increase vascular permeability in both large and small vessels. The mitogenic activity of VEGFs appears to be mediated by specific VEGF receptors.


AR483-10R



Angiosarcoma stained with Anti-VEGF using DAB chromogen

Clone: Polyclonal

Source: Rabbit

Immunogen: Human recombinant VEGF165

Specificity: VEGF




Xmatrx: HX031-YCDE

P


Villin

Villin is a cytoskeletal protein of microvilli of epithelial cell brush borders found in absorptive cells of the intestine and proximal renal tubes. Villin is a very specific marker for gastrointestinal tumors and adenocarcinomas of the pancreas. Other subsets of tumors stained with Villin are Merkel cell, lung (with rootlets), ovarian and kidney. It does not stain breast cancer. Used in a panel with CK7, CK20/CDX-2 and TTF-1, Villin can be very useful in differentiating colon adenocarcinoma from breast carcinoma and from lung adenocarcinoma.


AM525-10M



Intestine stained with Anti-Villin using DAB as chromogen

Clone: ID2C3

Isotype: IgG

Source: Mouse

Immunogen: Recognizes porcine, chicken and human villin from cellular extract of the intestinal Cell line HT29 and from human renal epithelial cells

Specificity: Villin

Localization: Cytoplasm/Brush border



Xmatrx: HX031-YCDE

P


Vimentin

Vimentin is the major intermediate filament in a variety of mesenchymal or mesenchymally derived non-muscle cell types. Vimentin is found in all types of sarcomas and lymphomas. Positive staining for vimentin is seen in most cells of fibrosarcomas, liposarcomas, malignant fibrous histocytomas, angiosarcomas, chondrosarcomas and lymphomas. When the vimentin antibody is used in combination with other antibodies as a panel, it can aid in the histological classification of normal and malignant tissues. This antibody immunohistochemically labels a variety of mesenchymal cells.


AM074-10MAX074-YCD




Skin stained with Anti-Vimentin Antibody using DAB chromogen

Clone: V9

Isotype: IgG1

Source: Mouse

Immunogen: Vimentin purified from porcine eye lens

Specificity: Vimentin


Pre-treatment: None

P


Vimentin, Non-Hematopoietic

LN6 recognizes a unique epitope of vimentin, a 60 kD protein, not expressed in cells of hematopoietic derivation. LN6 can be useful in the immunohistological study of soft tissue disorders. It reacts strongly with sarcomas, melanomas and meningomas. LN6 does not, however, stain leukocyte common antigen-positive tissues such as lymphomas and leukemias. In normal tissue, LN6 stains endothelium, muscle, fibroblasts, melanocytes, peripheral nerve, Sertoli cells, kidney mesangial cells and tubules, osteoblasts and periosteum. This antibody stains non-hematopoietic form of Vimentin in human sarcomas and normal cells of mesenchymal derivation but is nonreactive with cells of hematopoietic derivation.


AX163-YCD

MU163-UC


Concentrated:

Leiomyoma stained with anti-Vimentin using DAB chromogen

Clone: LN6

Isotype: IgM

Source: Mouse

Immunogen: Human Thymic Nuclei

Specificity: Non-hematopoietic vimentin


Pre-treatment: None

P


135

Primary Antibodies


VIP

Vasoactive intestinal peptide (VIP) is a 28 amino acid neuropeptide that has been isolated from various organs like intestine, the brain, upper respiratory and nasal mucosa, salivary glands, and the male and female genital tracts. It is also identifiable in human eosinophils, polymorphonuclear and mononuclear leucocytes. VIP is also known as a potent stimulant of mucous secretion, vasodilatation, and smooth muscle relaxation in bronchus and many other organs. According to various studies, VIP also has effects on the immune regulation. VIP is known to have inhibited the proliferative response of T lymphocytes to mercuric chloride, and inhibited natural killer (NK) cell function.

Intestine tissue stained with Anti-VIP using DAB as chromogen

Clone: Polyclonal

Isotype: IgG

Source: Rabbit

Immunogen: Synthetic peptide corresponding to full length mature vasoactive intestinal peptide conjugated to Keyhole Limpet Haemocyanin

Specificity: VIP




Xmatrx: HX031-YCDE


AR530-10R



P


Wilm’s Tumor

Wilm’s tumor protein is a transcription factor that contains four zinc-finger motifs, it is essential for correct mammalian urogenital development. The WT1 gene, located on chromosome 11p13, is inactivated in 5 to 10% of sporadic Wilms’ tumors and nearly 100% of Denys-Drash patients. The protein encoded by WT1 regulates transcription of other genes and can function both as a transcriptional activator and repressor. The WT1 gene is normally expressed in fetal kidney and mesothelioma, and its expression has been suggested as a marker for Wilm’s tumor and mesothelioma.

Wilm’s tumor stained with Anti-Wilm’s tumor using DAB chromogen

Clone: CAN-R9(IHC)-56-2

Isotype: IgG

Source: Rabbit

Immunogen: A recombinant protein corresponding to N-terminus of human WT

Specificity: Wilm’s tumor protein




Xmatrx: HX032-YCDE


AN584-10M



P


ZAP-70

ZAP-70 is an abbrevation for Zeta-chain-associated protein kinase 70 (70 is the molecular weight in kD). The protein is a member in the protein-tyrosine kinase family. ZAP-70 protein is expressed in leukemic cells of approximately 25% of Chronic Lymphocytic Leukemia (CLL) cases. ZAP-70 expression is an excellent surrogate marker for the distinction between the Ig-mutated (ZAP-70 negative) and Ig-unmutated (ZAP-70 positive) CLL subtypes and can identify patient groups with divergent clinical courses. The ZAP-70 positive Ig-unmutated CLL cases have a poorer prognosis.

Tonsil tissue stained with Anti-ZAP-70 using DAB chromogen

Clone: ZAP70-C3

Isotype: IgG2a

Source: Mouse

Immunogen: Human ZAP-70

Specificity: ZAP-70




Xmatrx: HX031-YCDE


AM544-10M



P


ZAP-70

ZAP-70 (70-kDa zeta-associated protein) is an intracellular tyrosine kinase discovered initially because of its role in T-cell signaling. This enzyme, which is phosphorylated on tyrosine residues upon T-cell antigen receptor (TCR) stimulation, functions in the initial step of TCR-mediated signal transduction in combination with the Src family kinases, Lck and Fyn. This enzyme is also essential for thymocyte development. Mutations in this gene cause selective T-cell defect, a severe combined.

Clone: YE291

Isotype: IgG

Source: Rabbit

Immunogen: ZAP-70 antigen

Specificity: ZAP-70




Xmatrx: HX031-YCDE/HX032-YCDET Cells showing ZAP-70 expression in tonsillar tissue using AEC as chromogen


Concentrated:


AN466-5M

AN466-10M

NU466-UC

FG-466N

P


Primary Antibodies


IHC Detection Systems

138


www.biogenex.com

Immunohistochemistry is a highly sensitive method that allows the localization of antigen within a cell or a tissue with high resolution. The method is based on the use of a primary antibody that specifically binds to its complementary antigen. The bound antibody may then be visualized by a variety of methods such as colorimetric end points.

BioGenex offers three basic types of IHC Detection Systems:

I. Super SensitiveTM Polymer-HRP IHC Detection System

II. Super SensitiveTM One-step Polymer-HRP Detection system

III.Super SensitiveTM Link-Label IHC Detection System

I. Super SensitiveTM Polymer-HRP IHC Detection SystemThis is a novel detection system using a non-biotin polymeric technology that makes use of two major components: Super Enhancer and a Poly-HRP reagent. As the system is not based on the biotin-avidin system, problems associated with endogenous biotin are completely eliminated. The technology allows excellent cell penetration ability for intense nuclear, cytoplasmic and membrane antigen staining, compared with other polymer-HRPs.

Benefits • Clean stain without endogenous biotin background

• High signal to noise ratio for intense stain

• Excellent sensitivity for weakly expressed antigens

• Universal system for rabbit and mouse antibodies

• Higher dilution of antibodies

Super Sensitive™ IHC Detection Systems

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Ready-to-use Detection System

Super Sensitive™ Polymer-HRP IHC Detection System/DAB

60 Slides Super Enhancer Reagent (6.0 ml), Polymer-HRP Reagent (6.0 ml),

Stable DAB Substrate Buffer (10 ml), Liquid DAB chromogen (2.0ml),

Negative Control for Mouse (3.0 ml), Negative Control Rabbit (3.0ml),

Peroxide Block (6.0 ml), Power Block™ (6.0 ml)

QD400-60KE

Ready-to-use Detection System (Large Volume)

Super Sensitive™ Polymer-HRP IHC Detection System/DAB Large Volume

500 Slides Super Enhancer Reagent (50 ml),

Polymer-HRP Reagent (50 ml),

Stable DAB Substrate Buffer (50 ml), Liquid DAB Chromogen (5.0ml)

QD420-YIKE

Ready-to-use Detection System (Mega Volume)

Super Sensitive™ Polymer-HRP IHC Detection System/DAB Mega Volume

1000 Slides Super Enhancer Reagent (100 ml), Polymer-HRP Reagent (100 ml),

Stable DAB Substrate Buffer (100 ml), Liquid DAB chromogen (10ml)

QD430-XAKE

Super Sensitive™ Polymer-HRP IHC Detection System Mega Volume

1000 Slides Super Enhancer Reagent (100 ml), Polymer-HRP Reagent (100 ml) QD440-XAKE

IA. Manual Use

II. Super Sensitive™ One-step Polymer-HRP Detection Systems†

The detection system is designed with proprietary technology, provides superior sensitivity, specificity and very short protocol. The innovative secondary antibody-polymer conjugate consisting of multiple small HRP active-site, enabling clean and intense, nuclear, cytoplasmic and membrane stain. This technology completely eliminates background staining caused by endogenous biotin in tissues especially kidney, liver, spleen, colon, stomach and brain.

Benefits• One system does it all - mouse and rabbit

• Easy and fast staining in 15 minutes

• Clean and intense stain without endogenous biotin background

• Higher dilution of antibodies



Super SensitiveTM One-step Polymer-HRP Detection Kit/DAB, Large Volume

500 Slides Polymer-HRP Reagent (50 ml),

Stable DAB Substrate Buffer (50 ml), Liquid DAB chromogen (5.0 ml)

QD620-YIKE

Ready-to-use Detection System (Mega Volume)

Super SensitiveTM One-step Polymer-HRP Detection Kit/DAB, Mega Volume

1000 Slides Polymer-HRP Reagent (100 ml), Stable DAB Substrate Buffer (100 ml), Liquid DAB chromogen (10 ml)

QD630-XAKE

IIA. Manual Use

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A classic system based on the highly specific and sensitive streptavidin-biotin interaction to detect a bound antibody. The immunohistochemical reaction takes place in following steps:

1. Cells or tissues are prepared and then incubated with an unlabeled primary antibody that will bind to the antigen.

2. The bound antibody is detected with a species-specific secondary antibody that will recognize a group of primary antibodies (e.g. primary antibodies made in rabbits). The secondary antibody is labeled with multiple biotin residues.

3. The bound secondary antibody is then allowed to react with Streptavidin conjugated with an enzyme. Streptavidin binds extremely strongly and irreversibly to the biotin residues on the secondary antibody resulting in the addition of multiple enzyme to the primary antibody complex.

4. A substrate is then added and acted upon by the enzyme producing a highly visible precipitate.

Benefits • Improved staining of weak antigens

• User-friendly and extensive choice of kit formats designed for use with human, animal or rodent tissue

• Wide choice of chromogens offered Super SensitiveTM Link-Label IHC Detection System is available in manual and automated formats.

III. Super SensitiveTM Link-Label IHC Detection Systems

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IIIA. Manual UseProduct Type Link Label Chromogen Pack size (Slides) Cat. No.

Ready to-Use MultiLink® HRP AEC 60 QP000-5LContents: Link (6 mL Biotinylated Anti-Immunoglobulins), Chromogen (AEC Liquid,) Negative Control (3 mL serum) Label (6mL Enzyme-Conjugated Streptavidin), Substrate, Peroxide Block (6 mL)

Large VolumeMultiLink® Alkaline Phosphatase 500 QA900-9LMultiLink® HRP 500 QP900-9L

Contents: Link (50 mL Biotinylated Anti-immunoglobulins) Label (50 ml Enzyme-Conjugated Streptavidin)

Mega Volume MultiLink® HRP 1000 QP300-XAK

Contents: Link (100 mL Biotinylated Anti-Immunoglobulins) Label (100 mL Enzyme-Conjugated Streptavidin)

ConcentratedMultiLink® Alkaline Phosphatase 1000+ LA000-ULEMultiLink® HRP 1000+ LP000-ULE

Contents: Link (5 mL Concentrated Biotinylated Anti-Immunoglobulins), Label (5 mL Concentrated Enzyme-Conjugated Streptavidin), Dilution Vials (2 each)

Ready to use MultiLink® HRP DAB/2 component 60 QD470-60KContents: Link (6 mL Biotinylated Anti-immunoglobulins), Chromogen 2 mL (DAB) Negative Control (3 mL x 2 serum) Label (6 mL Enzyme-Conjugated Streptavidin), Peroxide block (6 mL), Hematoxylin (6 mL)

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Links and LabelsReady-to-use LinksLinks are available as either species-specific or MultiLink® secondary antibodies. MultiLink® permits detection of mouse IgG+IgM and rabbit Ig primary antibodies, providing a universal link reagent for use with most monoclonal and polyclonal antibodies.

Concentrated LabelConcentrated Labels are supplied with recommended dilutions for use with BioGenex antibodies. Concentrated peroxidase labels are recommended to be diluted in Streptavidin Peroxidase Diluent (Cat. No. HK157-5K).


Concentrated HRP Label (Peroxidase-Conjugated Streptavidin) 5 ml HK320-UK

Concentrated AP Label (Alkaline Phosphatase-Conjugated Streptavidin) 5 ml HK321-UK

HRP Label

Ready-to-use LabelsRTU labels for biotin-streptavidin detection systems are compatible with the Super SensitiveTM Links. Labels include horseradish peroxidase and alkaline phosphatase conjugates.


SS HRP Label (Peroxidase-Conjugated Streptavidin) 6 ml HK330-5K 50 ml HK330-9K

SS AP Label (Alkaline Phosphatase-Conjugated Streptavidin) 6 ml HK331-5K 50 ml HK331-9K

Labels - Super SensitiveTM Systems

Links - Super SensitiveTM Systems Product Name Pack size Cat. No.

SS MultiLink® 6 ml HK340-5K* (Biotinylated Goat Anti-Immunoglobulins, for use with Mouse and 50 ml HK340-9K* Rabbit Primary Antibodies)

SS Mouse Link 6 ml HK335-5M (Biotinylated Goat Anti-mouse Immunoglobulins) 50 ml HK335-9M

SS Rabbit Link 6 ml HK336-5R (Biotinylated Goat Anti-rabbit Immunoglobulins) 50 ml HK336-9R

SS Goat Link 6 ml HK337-5G (Biotinylated Rabbit Anti-goat Immunoglobulins)

SS Rat Link 6 ml HK338-5T (Biotinylated Rabbit Anti-rat Immunoglobulins)

Concentrated LinkThe concentrated links are available in MultiLink® formats, and are supplied with recommended dilutions for use with BioGenex Antibodies. MultiLink® provides a universal link reagent for use with most monoclonal and polyclonal antibodies. Link Diluent (Cat. No. HK165-5K) should be used to dilute all concentrated links.


Concentrated MultiLink® 5 ml HK268-UK (Biotinylated Anti-immunoglobulins, for use with Mouse, Guinea Pig, Rat and Rabbit Primary Antibodies)

Concentrated Mouse Link 5 ml HK325-UM (Biotinylated Anti-mouse Immunoglobulins)

Concentrated Rabbit Link 5 ml HK326-UR (Biotinylated Anti-rabbit Immunoglobulins)

Concentrated SS Goat Link 5 ml HK327-UG (Biotinylated Rabbit Anti-goat Immunoglobulins)

MultiLink®

Multi-Staining

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BioGenex Double Staining IHC products include pre-optimized antibody cocktails and Super Sensitive multiple detection systems, enabling simultaneous testing of multiple antigens on single slide with a fast and easy protocol, assisting rapid and accurate diagnosis.

This double staining system is designed with novel polymer technology for fast and easy IHC staining of multiple antigens on a single slide. This system is pre-optimized for human tissues with superior sensitivity and specificity to produce precise and reliable results that allow easy interpretation and accurate diagnosis.

• Pre-mixed and pre-optimized polymer cocktails

• More patient data per slide – testing multiple protein biomarkers simultaneously

• Easy and fast – staining with a 4-step protocol

• Saving costs by maximizing resources

• Clean and intense stain without endogenous biotin background

Product Name Pack Size Contents Cat. No.

Ready-to-use Detection System

Super Sensitive™ Double Staining Polymer Detection Kit I / DAB & Permanent Fast Red

60 Slides

6ml Anti-Rabbit Polymer-HRP + Anti-Mouse Polymer-AP Cocktail, 12ml Peroxide Block, 12ml Power BlockTM, 6ml Mouse Negative control, 6ml Rabbit Negative Control, 4ml Liquid DAB chromogen, 10ml Stable DAB Substrate Buffer, 20 ml Permanent Fast Red A, 20ml Permanent Fast Red B

QS200-60K

Super Sensitive™ Double Staining Polymer Detection Kit II / DAB & Permanent Fast Red

60 Slides

6ml Anti-Rabbit Polymer-AP + Anti-Mouse Polymer-HRP Cocktail, 12ml Peroxide Block, 12ml Power BlockTM, 6ml Mouse Negative Control, 6ml Rabbit Negative Control, 4ml Liquid DAB chromogen, 10ml Stable DAB Substrate Buffer, 20ml Permanent Fast Red A, 20ml Permanent Fast Red B

QS400-60K


Super Sensitive™ Double Staining Polymer Detection Kit I Large Volume

500 Slides 50ml Anti-Rabbit Polymer-HRP + Anti-Mouse Polymer-AP Cocktail QS210-YIK

Super Sensitive™ Double Staining Polymer Detection Kit II Large Volume

500 Slides 50ml Anti-Rabbit Polymer-AP + Anti-Mouse Polymer-HRP Cocktail QS410-YIK

Double Staining

Super Sensitive™ Double Staining Polymer Detection System

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• Pre-mixed and pre-optimized antibody cocktails • More patient data per slide – testing multiple protein biomarkers simultaneously • Easy and fast – staining with a 4-step protocol • Saving costs by maximizing resources • Excellent sensitivity and high antibody efficiency

CD10 + Cyclin D1

CD10, also known as common acute lymphoblastic leukemia antigen (CALLA), is a cell surface enzyme which inactivates a variety of biologically active peptides. CD10 is expressed on the cells of lymphoblastic, Burkitt’s, and follicular germinal center lymphomas and in chronic myelogenous leukemia (CML). Cyclins are a family of protein that act as key regulators of the cell cycle. Cyclin D1 (CCND1) is involved in the G1/S transition of the cell cycle by activating Cdk4 and Cdk6 and inhibiting the retinoblastoma protein. Overexpression of Cyclin D1 has been associated with the development of many cancers including breast cancer, colon cancer, prostate cancer, and mantle cell lymphoma. Cyclin D1 and CD10 are useful in the differential diagnosis and confirmation of mantle cell lymphoma.

Breast carcinoma stained with anti-CD10+Cyclin D1

Source & Clone: Mouse 56C6 + Rabbit EPR2241(IHC)-32

Isotype: IgG1 + IgG

Localization: Membrane, Nucleus

Pre-treatment: EZ-ARTM 1, EZ-AR 2

Manual: HK521-XAK


Ready-to-use (Manual): AC561-5M

AC561-10MReady-to-use (Automated) i6000™:


AC561-YCD Xmatrx®:

Breast carcinoma

CD3 + CD20

CD3 is a lineage-specific “pan T-Cell” surface antigen compose of five different polypeptide chains. The CD3 antibody recognizes the human CD3 antigen which is present on mature human T-cells, thymocytes, and a subset of NK cells. CD20 is a non-glycosylated phosphoprotein expressed on the surface of all mature B-cells. The CD20 antibody primarily recognizes a 33 kD polypepitide B-cell component and a mono 30 kD cellular antigen. The CD3-CD20 cocktail reveals lymphoid architecture in neoplastic situations. CD20 and CD3 antibodies are provided at optimal concentrations in an easy-to-use format for visualizing T- and B-lymphocytes. This antibody cocktails can be used for the typing of malignant lymphomas and other lymphoproliferative disorders.

Tonsil stained with Anti-CD3 and Anti-CD20

Source & Clone: Rabbit EP449E + Mouse L-26

Isotype: IgG + IgG2a Kappa

Localization: Membrane & Cytoplasm, Membrane

Pre-treatment: EZ-AR TM 1, EZ-AR 2

Manual: HK521-XAK




Recommended Positive Control: Tonsil or spleen

AC559-YCD Xmatrx®:

CD4 + CD8

CD4 is a single chain transmembrane glycoprotein expressed on the surface of T helper cells and regulatory T-cells. CD4 is a co-receptor that assists the T-cell receptor (TCR) with an antigen-presenting cell. CD4 interacts directly with MHC class II molecules on the surface of the antigen-presenting cell. CD8 is a transmembrane glycoprotein that serves as a co-receptor for the T-cell receptor (TCR). The CD8 co-receptor is predominantly expressed on the surface of cytotoxic T-cells, but can also be found on natural killer cells and dendritic cells. CD8 binds to a major histocompatibility complex (MHC) molecule, but is specific for the class I MHC protein. CD4-CD8 double staining reveals the distribution of T-lymphocyte subsets, for example in HIV infection, infiltrating cells in graft rejection and lymphoma.

Source & Clone: Mouse 4B12 + Rabbit EP1150Y

Isotype: IgG1 + IgG


Pre-treatment: AR-10/ EZ-AR 1, EZ-AR 2





Recommended Positive Control: Tonsil adenocarcinoma

AC595-YCD Xmatrx®:

Tonsil stained with anti-CD4 + CD8

Source & Clone: Mouse CDX-2-88 + Rabbit EPR1619Y

Isotype: IgG1 Kappa + IgG

Localization: Nucleus, Cytoplasm

Pre-treatment: AR Citra Plus/EZ-AR 1, EZ-AR 2



CDX-2, a member of the caudal-related homeobox family, is an intestine-specific transcription factor that regulates both proliferation and differentiation in intestinal epithelial cells. It plays an important role in triggering cells toward the phenotype of differentiated villus enterocytes as well as in the maintenance of the phenotype. CDX-2 is used as a marker for gastrointestinal differentiation, especially colorectal. Cytokeratin 7 is a 54kD intermediate filament protein found in a variety of glandularepithelia. Cytokeratin 7 has been found in columnar and glandular epithelium of the lung, cervix, breast, bile ducts and larger collecting ducts of the kidney. CDX-2 and CK7 combination will help to distinguish the tumor origin from gastrointestinal, especially colorectal to adenocarcinomas of lung, breast, endometrioid tumors, and transitional cell carcinoma of the bladder.


Recommended Positive Control: Colon or breast carcinoma

CDX2 and CK7 stained in colon cancer metastasized into lung tissue

CDX-2 + CK7

AC596-10MReady-to-use (Automated) i6000™:AC596-YCD Xmatrx®:

Double StainingSuper Sensitive™ Antibody Cocktails

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Ki67 + Lambda

Ki67 is a nuclear protein present in cells at all phase of the cell cycle except G0. As such, Ki67 is a useful marker to identify the proliferation activity of cell populations. The staining of this activity, designated as the Ki67 labeling index, has shown to be clinically significant as a prognosis marker for breast, colorectal, skin cancer, and various lymphomas. The light chain is a polypeptide subunit of immunoglobulin expressed by B-cells. These B-cells are restricted to one of two subtypes of light chain, lambda or kappa. As a result, the light chain is a useful marker for lymphomas characterized as a monoclonal proliferation of B-cells. The Ki67 and lambda light chain cocktail is useful in evaluating cell proliferation of lambda light chain positive tumors.

Tonsil stained with anti-Ki67 + Lambda

Source & Clone: Mouse K-2 + Rabbit polyclonal

Isotype: IgG + Polyclonal


Pre-treatment: AR Citra Plus/ EZ-AR 1, EZ-AR 2





Recommended Positive Control: Tonsil

AC562-YCD Xmatrx®:

Source & Clone: Mouse G175-405 + Rabbit EPR3611

Isotype: IgG + IgG

Localization: Nucleus and/or Cytoplasm, Nucleus



p16/INK4A is a tumor-suppressor protein. The related genetic and epigenetic abnormalities in genes controlling the G1 checkpoint can lead to both escape from senescence and cancer formation. Ki-67 is a nuclear protein that is associated with and may be necessary for cellular proliferation. p16/Ki-67 immunostains are helpful to assess cervical biopsies for HPV-associated lesions. For research use only, not for use in diagnostic procedures.


Recommended Positive Control: Cervical adenocarcinoma

Cervical carcinoma stained with Anti-p16+Ki67

p16 + Ki67

RUO


TTF-1 + CK5&6

Thyroid transcription factor 1 (TTF-1) is a homeodomain-containing transcription factor that regulates the expression of thyroid and lung-specific genes. TTF-1 is detected in small cell carcinomas, neuroendocrine carcinomas, and thyroid carcinomas. As such, TTF-1 can help identify primary pulmonary carcinomas and differentiate it from metastatic carcinomas. Keratins are intermediate filaments found in the cytoskeleton of epithelial cells. Cytokeratin (CK) 5 and 6 are both type II keratins and are associated with the differentiation of simple and stratified epithelial tissue. CK 5/6 has been documented as a useful positive marker for diagnosing malignant mesothelioma. Together, TTF-1 and CK 5 /6 antibodies form valuable markers to help differentiate between mesotheliomas and pulmonary adenocarcinomas.

Lung squamous cell carcinoma stained with anti-TTF1+CK5&6

Source & Clone: Mouse 8G7G3/1+ Rabbit EPR1600Y+ Rabbit EPR1602Y

Isotype: TTF-1 IgG1 Kappa + CK5&6 IgG

Localization: TTF-1 Nucleus, CK5&6 Cytoplasm






Recommended Positive Control: Lung squamous carcinoma

AC563-YCD Xmatrx®:

Source & Clone: Mouse 8G7G3/1+Rabbit EP1582Y

Isotype: IgG1 Kappa + IgG


Pre-treatment: AR Citra/ EZ-AR 1, EZ-AR 2



Thyroid transcription factor-1 (TTF-1) is a sensitive marker for diagnosis of primary pulmonary adenocarcinoma, and differentiation between poorly differentiated squamous cell carcinoma and small cell carcinoma and adenocarcinoma. Gross cystic disease fluid protein (GCDFP-15) is currently used as an immunohistochemical marker of breast cancer. TTF-1/GCDFP-15 immunohistochemical profile in lung tumors is highly suggestive of metastatic carcinoma of the breast. In distinguishing metastatic breast carcinoma and adenocarcinoma of the lung, the cytoplasmic staining would indicate breast carcinoma and nuclear staining would indicate lung or thyroid carcinoma.


Recommended Positive Control: Lung squamous carcinoma

Lung squamous cell carcinoma stained with Anti-TTF1 +GCDFP-15

TTF-1 + GCDFP-15


Double Staining

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Double StainingCDX-2 + CK20 + CK7

Source & Clone: Mouse CDX-2-88 + Rabbit EPR1622Y + Rabbit EPR1619Y

Isotype: IgG1 Kappa + IgG + IgG

Localization: CDX-2 Nucleus, CK20 Cytoplasm, CK7 Cytoplasm





Recommended Positive Control: Colon or breast carcinoma

Colon carcinoma stained with Anti-CDX-2+CK20+CK7

CDX-2, a member of the caudal-related homeobox family, is an intestine-specific transcription factor that regulates both proliferation and differentiation in intestinal epithelial cells. CDX-2 is used as a marker for gastrointestinal differentiation, especially colorectal. Cytokeratin 7 has been found in columnar and glandular epithelium of the lung, cervix, breast, bile ducts and larger collecting ducts of the kidney. CK7 expression is significantly more frequent in adenocarcinomas of pulmonary and breast origin than gastrointestinal (GI) origin, In contrast, CK20 expression is significantly more prevalent in gastrointestinal adenocarcinoma than that of pulmonary or breast origin. CDX-2/CK20/CK7 combination will help to distinguish the tumor origin from gastrointestinal, especially colorectal to adenocarcinomas of lung, breast, endometrioid tumors.


Source & Clone: Mouse 4A4 + Rabbit EPR1600Y

Isotype: IgG




Xmatrx: HX031-YCDE HX032-YCDE

Tumor protein p63 is a member of the p53 family of transcription factors. Cytokeratin 5 (58 kD) is expressed in basal, intermediate and superficial cell layers of stratified epithelia as well as transitional epithelia, and in mesothelial cells and mesothelioma. Cytokeratin 6 (56 kD) is also a high molecular weight cytokeratin expressed by proliferating squamous epithelium. p63 and cytokeratin (CK 5/6) are markers of basal and squamous differentiation in several normal epithelia and human tumors. p63 and cytokeratin (CK 5/6) are also helpful in distinguishing poorly differentiated squamous cell carcinoma from small cell carcinoma or adenocarcinoma. Simultaneous detection of p63, CK5/6 can help increase the diagnostic accuracy of breast diseases.



Recommended Positive Control: Prostate adenocarcinoma

AC602-YCD Xmatrx®:

Prostate tissue stained with Anti-p63+CK5

p63 + CK5

PIN4 (p63 + CK HMW + p504S)

This antibody cocktail recognizes Prostate Intraepithelial Neoplasia (PIN) in the tissues stained by immunohistochemical techniques. A cocktail of these three antibodies might allow simultaneous demonstration of P504S, HMW CK and p63 using a single immunostain. The combination of P504S + HMW CK + p63 (PIN4 Cocktail) may be extremely useful for studying prostatic intraepithelial neoplasia, especially in difficult cases and in cases with limited tissue. For Research Use only, not for use in diagnostic procedures. For research use only. Not for use in diagnostic procedures.

Prostate carcinoma. stained with anti-PIN4

Source & Clone: Mouse 4A4 + Mouse 34ßE12 + Rabbit 13H4

Isotype: IgG

Localization: p63 Nucleus, CK HMW and p504S Cytoplasm



Xmatrx: HX032-YCDE


AM448-10MEReady-to-use (Automated) i6000™:

Recommended Positive Control: Prostate adenocarcinoma

AX448-YCDE Xmatrx®:

MU448-UCEConcentrated:RUO

Source & Clone: Mouse 8G7G3/1+ Rabbit EPR1622Y + Rabbit EPR1619Y

Isotype: IgG1 Kappa + IgG + IgG

Localization: TTF-1 Nucleus, CK20 Cytoplasm, CK7 Cytoplasm




Thyroid transcription factor-1 (TTF-1) is a sensitive marker for the diagnosis of primary pulmonary adenocarcinoma, and differentiation between poorly differentiated squamous cell carcinoma and small cell carcinoma and adenocarcinoma. CK7 expression is significantly more frequent in adenocarcinomas of pulmonary and breast origin than gastrointestinal (GI) origin, In contrast, CK20 expression is significantly more prevalent in gastrointestinal adenocarcinoma than that of pulmonary or breast origin. TTF-1/CK20/CK7 is a useful marker for diagnosis of lung tumors and for differential diagnosis of primary pulmonary adenocarcinomas from extrapulmonary adenocarcinomas metastatic to the lung. Application of this panel of antibodies might increase the accuracy of diagnosis.


Recommended Positive Control: Lung carcinoma

Lung/Breast carcinoma stained with Anti-TTF-1+CK20+CK7

TTF-1 + CK20 + CK7


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ISH Probes & Detection Systems

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BioGenex offers fluorescein-labeled oligonucleotide probes for the detection of RNA or DNA by in situ Hybridization (ISH). These probes allow the localization of specific nucleic acid sequences within cells from formalin-fixed, paraffin-embedded tissue sections. When used with the BioGenex ISH Detection systems, these probes offer reliable, highly sensitive and easy-to-perform DNA and RNA assays.

Alu II Probe

Alu, an important group of widely distributed sequences repeated in the human genome, has been widely used in in situ hybridization technique. Analyte Specific Reagent. Analytical and performance characteristics are not established. Automated versions are for research use only.

Ready-to-use (Manual): PR026-100E(ASR)

Specificity: Alu II DNA

Ready-to-use (Automated):

Xmatrx: PR026-YADE (RUO)

Alu sequence detected in FFPE tissue stained with DAB

Beta-Actin*

Actins are highly conserved proteins that participate in cell motility as well as cell structure and integrity. In normal cells, beta-actin mRNA is localized in cell protrusions where actin is actively polymerized. Analyte Specific Reagent. Analytical and performance characteristics are not established. Automated versions are for research use only.

Ready-to-use (Manual): PR1055-100E (ASR)

Specificity: Beta-Actin RNAReady-to-use Automated):


Beta actin mRNA staining of breast cancer tissue

CerviPro HPV 14 DNA Probe

The HPV 14 probe has been designed to specifically recognize regions of the L1 and E6/E7 open reading frames (ORFs) of human papillomavirus (HPV) 14 genotypes (HPV 16,18,31,33,35,39,45,51,52,56,58,59,66,68)in paraffin embedded human tissues or cytopathology specimens/cervical scraps. Analyte Specific Reagent. Analytical and performance characteristics are not established. Automated versions are for research use only.


Specificity: HPV 14 genotypes



HPV 14 HR genotype in Ca Cervix tissue stained with DAB

CerviPro HPV Type 16/18 DNA Probe

The CerviPro HPV Type 16/18 DNA probe has been designed to recognize regions of the E1, E6, L1, and L2 open reading frames (ORFs) of human papillomavirus (HPV) genotypes in paraffin embedded human tissues or cytopathology specimens/cervical scraps. Analyte Specific Reagent. Analytical and performance characteristics are not established. Automated versions are for research use only.


Specificity: HPV viral DNA sequences



HPV16/18 in Ca Cervix stained with DAB

Cyclin D1 Probe*

Cyclin D1, also known as PARD-1 or bcl1, is a G1 phase cyclin, which regulates G1 phase progression and G1/S transition of the cell cycle. Cyclin D1 mRNA is overexpressed in many cancer types including colon cancer, gastric cancer and breast cancer. Its overexpression is correlated with poor prognosis. Analyte Specific Reagent. Analytical and performance characteristics are not established. Automated versions are for research use only.


Specificity: Cyclin D1 RNA



Cyclin D1 mRNA in human Lymphoma tissue stained with DAB

EBV-Encoded RNA (EBER) Probe

Epstein-Barr virus-encoded RNA, EBER, is present in cells latently infected with Epstein-Barr virus (EBV). Analyte Specific Reagent. Analytical and performance characteristics are not established. Automated versions are for research use only.


Specificity: EBV-encoded RNA

Ready-to-use Automated):


Epstein-Barr early RNA (EBER) stained with DAB

In situ Hybridization Probes

* To be released soon

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Kappa Probe

The light chains of immunoglobulin molecules have two antigenic types: kappa and lambda. A given immunoglobulin molecule contains two identical light chains, either kappa or lambda. Therefore, the clonal nature of any immunoglobulin producing cell population can be determined by the light chain structure of the immunoglobulin that the cell produces. Analyte Specific Reagent. Analytical and performance characteristics are not established. Automated versions are for research use only.


Specificity: Kappa light chain mRNA



Human immunoglobulin kappa light chain mRNA in tonsil stained with DAB

Lambda Probe

The light chains of immunoglobulin molecules have two antigenic types: kappa and lambda. A given immunoglobulin molecule contains two identical light chains, either kappa or lambda. Therefore, the clonal nature of any immunoglobulin producing cell population can be determined by the light chain structure of the immunoglobulin that the cell produces. Analyte Specific Reagent. Analytical and performance characteristics are not established. Automated versions are for research use only.


Specificity: Lambda light chain mRNA



Human immunoglobulin lambda light chain mRNA in tonsil stained with DAB

Oligo d (T) Probe*

In all living cells, the expression of genetic information involves transcription of RNA molecules. The initial transcripts named heterogeneous nuclear RNA (hnRNA) are processed into mature messenger RNA (mRNA) by removing non-coding intron sequences and adding the 5’-methyl cap and a 3’-tail of approximately 200 adenylyl residues (poly (A)). In general, mRNA are conserved in routine formalin-fixed, paraffin-embedded tissues which have been fixed promptly. However, mRNA is not stable and may be destroyed during tissue processing of a routine formalin-fixed, paraffin-embedded tissue specimen. in situ hybridization with an oligo-d (T) probe is commonly used to assess the preservation of mRNA in a formalin-fixed, paraffin embedded tissue specimen. Analyte Specific Reagent. Analytical and performance characteristics are not established. Automated versions are for research use only.


Specificity: mRNA



Preservation of oligo d (T) mRNA in FFPE tissue stained with DAB

Retinoblastoma (RB) Probe*

The retinoblastoma tumor suppressor gene, RB, encodes a protein of 110 KD that plays an important role in cell growth regulation. Alterations in Retinoblastoma (RB1) mRNA expression have been reported in many human tumor types including lung cancer, osteosarcomas, leukemias, prostate cancer and bladder cancer. Increased expression of RB1 mRNA has been reported for many human colon tumor tissues and human colorectal cancer cell lines and Breast cancer. Analyte Specific Reagent. Analytical and performance characteristics are not established. Automated versions are for research use only.


Specificity: retinoblastoma tumor suppressor gene



Retinoblastoma mRNA in Adenocarcinoma tissue stained with DAB

Product Name Pack size Intended Use Cat. No. (Manual) Cat. No. (Automated)

Alu II Probe 25 slides Alu II sequences PR026-100E PR026-YADE

Beta Actin* 25 slides Initial standard PR1055-100E PR1055-YADE

CerviPro HPV 14 25 slides L1 and E6/E7 ORFs of HPV14 PR251-100E PR251-YADE

CerviPro HPV Type 16/18 25 slides E1, E6, L1, and L2 open reading frames (ORFs) of HPV PR250-100E PR250-YADE

Cyclin D1* 25 slides Cyclin D1, a G1 phase cyclin PR216-100E PR216-YADE

EBER Probe 25 slides EBV-encoded RNA PR205-100E PR205-YADE

Kappa Probe 25 slides Kappa light chain mRNA PR214-100E PR214-YADE

Lamda Probe 25 slides Lambda light chain mRNA PR215-100E PR215-YADE

Oligo d (T) Probe* 25 slides mRNA PR217-100E PR217-YADE

Retinoblastoma (RB) Probe* 25 slides Retinoblastoma tumor suppressor gene PR225-100E PR255-YADE

ISH ProbesProbes are packaged with RFID vials for staining up to 100 slides.

CerviPro HPV Type 16/18 DNA Probe

The CerviPro HPV Type 16/18 DNA probe has been designed to recognize regions of the E1, E6, L1, and L2 open reading frames (ORFs) of human papillomavirus (HPV) genotypes in paraffin embedded human tissues or cytopathology specimens/cervical scraps. Analyte Specific Reagent. Analytical and performance characteristics are not established. Automated versions are for research use only.


Specificity: HPV viral DNA sequences



HPV16/18 in Ca Cervix stained with DAB

Cyclin D1 Probe*

Cyclin D1, also known as PARD-1 or bcl1, is a G1 phase cyclin, which regulates G1 phase progression and G1/S transition of the cell cycle. Cyclin D1 mRNA is overexpressed in many cancer types including colon cancer, gastric cancer and breast cancer. Its overexpression is correlated with poor prognosis. Analyte Specific Reagent. Analytical and performance characteristics are not established. Automated versions are for research use only.


Specificity: Cyclin D1 RNA



Cyclin D1 mRNA in human Lymphoma tissue stained with DAB

EBV-Encoded RNA (EBER) Probe

Epstein-Barr virus-encoded RNA, EBER, is present in cells latently infected with Epstein-Barr virus (EBV). Analyte Specific Reagent. Analytical and performance characteristics are not established. Automated versions are for research use only.


Specificity: EBV-encoded RNA

Ready-to-use Automated):


Epstein-Barr early RNA (EBER) stained with DAB


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MicroRNA ProbesMicroRNAs (miRNAs) are endogenous, non-coding RNAs known to regulate gene expression by translational repression or RNA cleavage. Since miRNA has been observed to deregulate during progression of different cancer stages from normal to malignant and metastasis, the expression profile as a result of this deregulation can be exploited as a potential biomarker for cancer characterization.

BioGenex MicroRNA Probes

Automated Protocols• Optimized for automated ISH staining by Xmatrx ELITE • Ready-to-use reagents for FFPE tissues

Highly Specific and Sensitive Probes• Proprietary technology for clean intense stains• in situ context of tissue morphology

Manual Protocols • Optimized for standardized manual ISH staining • Ready-to-use reagents for FFPE tissues

Hsa-miR-1

The Hsa-miR-1probe has been designed from mature human miR-1 sequence. This fluorescenated probe is provided in a hybridization buffer for localization of miRNA in FFPE tissue by in situ hybridization. Analyte Specific Reagent. Analytical and performance characteristics are not established.

Ready-to-use (Manual): HM001-100E

Specificity: miR-1

Hsa-miR-1 detected in FFPE tissue stained with DAB

Hsa-miR-10b

The Hsa-miR-10b probe has been designed from mature human miR-10b sequence. This fluorescenated probe is provided in a hybridization buffer for localization of miRNA in FFPE tissue by in situ hybridization. Analyte Specific Reagent. Analytical and performance characteristics are not established.

Ready-to-use (Manual): HM010B-100E

Specificity: miR-10b

Hsa-miR-10b detected in FFPE tissue stained with DAB

Hsa-miR-17

The Hsa-miR-17 probe has been designed from mature human miR-17 sequence. This fluorescenated probe is provided in a hybridization buffer for localization of miRNA in FFPE tissue by in situ hybridization. Analyte Specific Reagent. Analytical and performance characteristics are not established.


Specificity: miR-17


Hsa-miR-200a

The Hsa-miR-200a probe has been designed from mature human miR-200a sequence. This fluorescenated probe is provided in a hybridization buffer for localization of miRNA in FFPE tissue by in situ hybridization. Analyte Specific Reagent. Analytical and performance characteristics are not established.

Ready-to-use (Manual): HM200a-100E

Specificity: miR-200a

Hsa-miR-200a detected in FFPE tissue stained with DAB

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Hsa-miR-21



Specificity: miR-21

Hsa-miR 21 detected in FFPE tissue stained with DAB

Hsa-miR-106a


Ready-to-use (Manual): HM106A-100E



Hsa-miR-125b

The Hsa-miR-125bprobe has been designed from mature human miR-125b sequence. This fluorescenated probe is provided in a hybridization buffer for localization of miRNA in FFPE tissue by in situ hybridization. Analyte Specific Reagent. Analytical and performance characteristics are not established.




Hsa-miR-126



Specificity: miR126


Hsa-miR-141



Specificity: miR141


Hsa-miR-144



Specificity: miR-144


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Hsa-miR-146a

The Hsa-miR-146a probe has been designed from mature human miR-146a sequence. This fluorescenated probe isprovided in a hybridization buffer for localization of miRNA in FFPE tissue by in situ hybridization. Analyte Specific Reagent. Analytical and performance characteristics are not established.




Hsa-miR-147b



Specificity: miR147b

Hsa-miR147b detected in FFPE tissue stained with DAB

Hsa-miR-150





Hsa-miR-151a-3p

The Hsa-miR-151a-3p probe has been designed from mature human miR-10b sequence. This fluorescenated probe is provided in a hybridization buffer for localization of miRNA in FFPE tissue by in situ hybridization. Analyte Specific Reagent. Analytical and performance characteristics are not established.


Specificity: miR151a-3p

Hsa-miR-151a-3p detected in FFPE tissue stained with DAB

Hsa-miR-152



Specificity: miR152


Hsa-miR-155




Has-miR 155 detected in FFPE tissue stained with DAB

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Hsa-miR-196a





Hsa-miR-200b





Hsa-miR-204





Hsa-miR-205





Hsa-miR-216a



Specificity: mIr-216a


Hsa-miR-222





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Hsa-miR-328





Hsa-miR-375



Specificity: miR375


Hsa-miR-1285



Specificity: miR1285

Hsa-miR1285 detected in FFPE tissue stained with DAB

Hsa-miR-let-7a

The Hsa-let-7a probe has been designed from mature human let-7a sequence. This fluorescenated probe is provided in a hybridization buffer for localization of miRNA in FFPE tissue by in situ hybridization. Analyte Specific Reagent. Analytical and performance characteristics are not established.


Specificity: LET-7a

Hsa-miR-let-7a detected in FFPE tissue stained with DAB

Scramble probe

The scramble probe sequence does not share homology with miRNA sequences available in the miRBase database. Scramble probe is used as a negative control during miRNA ISH. Analyte Specific Reagent. Analytical and performance characteristics are not established.

Ready-to-use (Manual): PR032-100E

Specificity: Negative control for ISH assays

Negative staining of scrambled probe in FFPE tissue

U6 probe

U6 snRNA is the non-coding small nuclear RNA (snRNA) component of U6 snRNP (small nuclearribonucleoprotein). The U6 snRNA sequence is highly conserved and the function of the U6 snRNA has remainedcrucial and unchanged through evolution. The U6 cellular transcript is available in abundance with intranuclear distribution in cell/tissue. The U6 probe is designed to bind to human U6 small nuclear RNA. Analyte Specific Reagent. Analytical and performance characteristics are not established.

Ready-to-use (Manual) PR031-100E

Specificity human U6 small nuclear RNA

U6 detected in FFPE tissue stained with DAB

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Hsa-miR-1 probe HM001-100E

Hsa-miR-10b probe HM010B-100E



Hsa-miR-106a probe HM106A-100E



Has-miR-141 probe HM141-100E





Hsa-miR-151-3p probe HM151-100E













Hsa-miR-let7a probe HM007A-100E

Scramble probe PR032 -100E

U6 probe PR031-100E

miRNA Probes

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Super SensitiveTM Polymer HRP ISH Detection System This is a novel detection system using a non-biotin polymeric technology that makes use of two major components : Super Enhancer and a Poly-HRP reagent. As the system is not based on the Biotin-Avidin System, problems associated with endogenous biotin are completely eliminated. The technology allows excellent cell penetration ability for intense staining, compared with other polymer HRPs.

Benefits• Clean Stain without endogenous biotin background• High signal to noise ratio for intense stain• Universal system for all fluorescein labeled probes

Product Name Pack Size Probe Type Cat. No.

Super Sensitive™ One Step Polymer-HRP ISH Detection System

1 x 5 ml Power BlockTM, 1 x 5 ml Peroxide Block,

1 x 10 ml DAB Buffer, 1 x 2.5 ml Liquid DAB Chromogen, 1 x 3 ml One step Poly-HRP Reagent;

1 x 10 ml Hematoxylin; 1 x 5 ml Proteinase K;

1 x 3 ml Nucleic Acid Retrieval 1 Solution;

1 x 20 ml Wash Solution A;1 x 20ml Wash Solution B; 1 x 20 ml Wash Solution E; 1 x 0 ml Wash Solution F; 1 x 3 ml Anti-Flourescein Antibody

50 Slides Fluorescein Labeled DF400-50KE

XISH One Step Polymer-HRP ISH Detection System

1 x 10 ml Power Block, 1 x 10 ml Peroxide Block, 4 x 5 ml DAB Buffer, 1 x 3 ml Liquid DAB Chromogen, 1 x 3 ml One-step Poly-HHP Reagent; 1 x 10 ml Hematoxyline; 1 x 3 ml Proteinase K; 1 x 3 ml Nucleic Acid Retrieval Solution; 2 x 10 ml Wash Solution E; 2 x 10 ml Wash Solution E; 2 x 10 ml Wash Solution F; 1 x 3 ml Anti-Flourescein Antibody

50 slides Fluorescein Labeled DF400-YADE

Hybridization Detection Systemin situ Hybridization (ISH) is a powerful technique for detecting and localizing specific nucleic acid sequences within cells or tissues. This is achieved by the hybridization of a labeled probe to the specific RNA/DNA sequence within the cell and subsequent detection of the bound probe.

Features • Enable the semi-quantification of mRNA expression and helps to determine the temporal and spatial patterns of gene expression in

cells, tissue and whole animals • Used for detecting intracellular pathogens with a very high degree of sensitivity

eFISHiency

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eFISHiency: Comprehensive high-throughput automated FISH processing systemsBioGenex offers eFISHiency system, a complete solution for cytogenetic FISH laboratory requirements under one umbrella, consisting of eFISH probes, pretreatment kits and high-throughput automated/semi-automated platforms.

eFISHiencyFluorescence in situ hybridization (FISH) is a robust technique of cytogenetic used for the detection of chromosomal aberrations viz., deletions, amplification and translocation in tissue sections or within individual cells in native context. In this technique florescent probes bind to the target sequence of DNA chromosome. High specificity and sensitivity coupled with rapid and accurate result has proven the role of FISH in both research and diagnosis of solid tumor and hematological malignancies. FISH is also used in genetic counseling, medicine and species identification. FISH can also be used to detect and localize specific RNA targets in cells, circulating tumor cells and tissue samples.

In an FISH procedure, fixed tissue sections/cytology specimens are pretreated to expose target DNA or mRNA sequences. An appropriately labeled probe is hybridized to the exposed target in the cells, followed by stringency washing steps to remove non-specifically bound probe. Subsequently slides are mounted using DAPI/antifade and can be visualized under fluorescence microscope using appropriate filter set.

Sr # eFISHiency Components Description

1 eFISH probes More than 50 FISH probes covering major genetic aberrations Probes for detection and diagnosis of genetic aberrations

2 eFISH kits

eFISH Histo eFISH kit for histology FFPE tissue samples

eFISH Cyto eFISH kit for cytology specimens

eFISH U eFISH kit for manual usage - both histology and cytology specimens.

3 eFISH processing systems

Xmatrx® ELITE

World’s only high-throughput front end FISH processing system that process FISH slides from microtome to microscope including final coverslipping.

40 different protocols in combination of histology and cytology specimens/probes can be processed at a time.

Xmatrx® NANO

10 slides semi-automated work station for small size FISH laboratory requirement with provision of manual pipetting of FISH probes, DAPI and costly reagents.

10 different protocols in combination of histology and cytology specimens/probes can be processed at a time

Xmatrx® MINI10 slides manual FISH processing platform with provision of on board pretreatment, dewaxing and washing. 10 different protocols in combination of histology and cytology specimens/probes can be processed at a time

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1. eFISHiency probesWe offer a wide range of probes for cytology specimens and histology tissues.

Detection and Diagnosis of Genetic Aberrations

Locus-Specific Probes• Bind to specific small sequences on chromosomes• Detect structural abnormalities (e.g. gene amplifications, deletions and duplications)

Alphoid or Centromeric Repeat Probes • Detect repetitive sequences found in middle of each chromosome• Serve as enumeration probe to determine gene copy and chromosome numbers

Whole Chromosome Probes• Apply single or multi-color probes to label each chromosome in its own unique color• Include gene fusion and break-apart probes to assess translocations and rearrangements

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Sr # eFISH Probes Cat. No.

1 eFISH MDM2/CEN 12 Dual Color Probe FP038-10XE/20XE

2 eFISH CEN 17/HER2 Dual Color Probe FP039-10XE/20XE

3 eFISH EGFR/CEN 7 Dual Color Probe FP040-10XE/20XE

4 eFISH p16/CEN 9 Dual Color Probe FP041-10XE/20XE

5 eFISH FGFR1/CEN 8 Dual Color Probe FP042-10XE/20XE

6 eFISH NMYC/2q11 Dual Color Probe FP043-10XE/20XE

7 eFISH 1p36/1q25 Dual Color Probe FP044-10XE/20XE

8 eFISH 19q13/19p13 Dual Color Probe FP045-10XE/20XE

9 eFISH VHL/CEN 3 Dual Color Probe FP046-10XE/20XE

10 eFISH MET/CEN 7 Dual Color Probe FP047-10XE/20XE

11 eFISH EWSR1 Dual Color Break Apart Probe FP048-10XE/20XE

12 eFISH SYT Dual Color Break Apart Probe FP049-10XE/20XE

13 eFISH CHOP Dual Color Break Apart Probe FP050-10XE/20XE

14 eFISH TFE3 Dual Color Break Apart Probe FP051-10XE/20XE

15 eFISH COL1A1/PDGFB Dual Color Dual Fusion Probe FP052-10XE/20XE

16 eFISH PDGFB Dual Color Break Apart Probe FP053-10XE/20XE

17 eFISH COL1A1 Dual Color Break Apart Probe FP054-10XE/20XE

18 eFISH FGFR2/CEN 10 Dual Color Probe FP055-10XE/20XE

19 eFISH ALK Dual Color Break Apart Probe FP056-10XE/20XE

20 eFISH CCND1/IGH Dual Color Dual Fusion Probe FP057-10XE/20XE

21 eFISH FUS Dual Color Break Apart Probe FP058-10XE/20XE

22 eFISH PIK3CA/CEN 3 Dual Color Probe FP059-10XE/20XE

23 eFISH ROS1 Dual Color Break Apart Probe FP060-10XE/20XE

24 eFISH RET Dual Color Break Apart Probe FP061-10XE/20XE

25 eFISH TP53/CEN 17 Dual Color Probe FP062-10XE/20XE

26 eFISH CCND1/CEN 11 Dual Color Probe FP063-10XE/20XE

27 eFISH CMYC Dual Color Break Apart Probe FP064-10XE/20XE

28 eFISH CMYC/CEN 8 Dual Color Probe FP065-10XE/20XE

29 eFISH TERT/5q31 Dual Color Probe FP066-10XE/20XE

30 eFISH CMYC/IGH Dual Color Dual Fusion Probe FP067-10XE/20XE

31 eFISH EGR1/5p15 Dual Color Probe FP068-10XE/20XE

32 eFISH CCND1 Dual Color Break Apart Probe FP069-10XE/20XE

33 eFISH IGH Dual Color Break Apart Probe FP070-10XE/20XE

34 eFISH BCR/ABL Dual Color Dual Fusion Probe FP071-10XE/20XE

35 eFISH AML1/ETO Dual Color Dual Fusion Probe FP072-10XE/20XE

36 eFISH PML/RARA Dual Color Dual Fusion Probe FP073-10XE/20XE

37 eFISH BCL2/IGH Dual Color Dual Fusion Probe FP074-10XE/20XE

38 eFISH BIRC3/MALT1 Dual Color Dual Fusion Probe FP075-10XE/20XE

39 eFISH ETV6/RUNX1 Dual Color Dual Fusion Probe FP076-10XE/20XE

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2. eFISH pretreatment kitsSr # eFISH Kit Description Automation Platform

1 eFISH Histo eFISH Kit for Histology tissue (FFPE) Xmatrx, NANO and MINI

2 eFISH Cyto eFISH Kit for Cytology Specimen Xmatrx, NANO and MINI

3 eFISH U eFISH Kit Universal for both histology and cytology specimen Manual

I. eFISH Histo - Cat # DF500-20XEeFISH Histo is designed to meet the FISH processing requirement of FFPE tissues. It contains buffers and reagents for pretreatment and post hybridization stringency washes. This kit is compatible on automation platforms, Xmatrx ELITE, Xmatrx NANO and Xmatrx MINI.

Sr # Reagents Volume/amount Part No.

1 eFISH pretreatment Solution 20 ml HK983-20XE

2 eFISHProtease 1 Buffer 5X20 ml HK981-20XE

3 eFISH reagents A 20 ml HK972-20XE

4 Pepsin powder 1 5X10 mg HK602-10XE

5 eFISH Wash buffer 1 (20X) 200 ml HK604-20XE


1 eFISH reagents A 20 ml HK972-20XE

2 eFISH reagents B 20 ml HK973-20XE

3 eFISH Protease 2 Buffer 5X20 ml HK982-20XE

4 eFISH Formaline Fixative 20 ml HK603-20XE

5 Pepsin powder 2 5X2 mg HK602-02XE



II. eFISH Cyto - Cat # DF510-20XEeFISH Cyto is designed to meet the FISH processing requirement of cytology and hematology specimens. It contains buffers and reagents for pretreatment and post hybridization stringency washes. This kit is compatible on automation platforms, Xmatrx ELITE, Xmatrx NANO and Xmatrx MINI


1 eFISH pretreatment Solution 500 ml HK983-YRXE

2 eFISH reagents A 500 ml HK972-YRXE

3 eFISH reagents B 500 ml HK973-YRXE

4 eFISH Protease 1 Buffer 500 ml HK981-YRXE

5 eFISH Protease 2 Buffer 500 ml HK982-YRXE

6 eFISH Wash buffer 1 (10X) 50 ml HK604-YRXE

7 eFISH Wash buffer 2 (10X) 50 ml HK605-YRXE

8 eFISH Formaline Fixative 500 ml HK603-YRXE

9 Pepsin powder 1U 25 mg X 10 HK602-25KE

10 Pepsin powder 2 U 5 mg X 10 HK602-05KE

III. eFISH U - Cat # DF-530-40KEeFISH U, is a bulk reagents universal kit, designed to meet the FISH processing requirement of both histology FFPE tissue and cytology/hematology specimen. It contains buffers and reagents for pretreatment and post hybridization stringency washes.

33 Steps Reduced to 3 Steps7.5 Hours Reduced to 30 Minutes

Microtome to Microscope eFISHiency System for FISH Automation

COMPLETE AUTOMATION33 Steps Reduced to 3 Steps7.5 Hours Reduced to 30 Minutes

FISH - AUTOMATION33 Steps Reduced to 6 Steps7.5 Hours Reduced to 1 Hour


eFISHiency WORKSTATION33 Steps

7.5 Hours Reduced to 5.5 Hours

MANUAL33 Steps7.5 Hours

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3. eFISH Processing systemsTrue eFISHiencyNow FISH can be the nexus of a more efficient and more productive laboratory. With a family of Xmatrx® systems, you have the freedom to attend to more demanding tasks while delivering high-quality and standardized results every time.

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• The world’s first and only fully automated front-end FISH processing system • Run up to 40 slides under multiple protocols• Reduce hands-on tech time from 7.5 hours to 30 minutes



• On-board dewaxing, oil seal and final coverslip after DAPI• Add micro-reagents manually to save cost• Run 10 different protocols at the same time• Intelligent SMS information for alerts



• eFISHiency Workstation for manual FISH assay• Hybridizer with eXACTTM temperatures• 10 Independently programmable thermal cyclers• Built-in touch screen display• Manual coverslip application and removal

Accessories

Oil stamp Coverslip stand Suction pen

Rethink the way FISH fits into your workflow

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Family of Xmatrx® Systems to Provide Optimum Workflow Solutions for Your Laboratory NeedsWith superior staining quality and enhanced laboratory productivity in mind, we have developed a family of Xmatrx® Systems to produce standardized results and provide optimum workflow solutions for your laboratory needs by:

•Streamlining lab workflow •Increasing throughput •Improving reproducibility • Freeing up critical resources

Placement of slides on eXACTTM temperature controlled blocks

Automated application of oil for sealing reaction chamber (micro-chamber)

Automated dispensing of micro-reagents (proteinase, probe and DAPI)

Automated application and removal of coverslips

Automated wash and airblow to dry slides

Automated mounting and final coverslip after DAPI


Automated application of oil for sealing reaction chamber (micro-chamber)

Manual dispensing of micro-reagents (proteinase, probe and DAPI)

Automated application and removal of coverslips

Automated wash and airblow to dry slides

Automated mounting and final coverslip after DAPI


Manual application of oil with an oil stamp for sealing reaction chamber [micro-chamber]

Manual application and removal of coverslips with a suction pen

Manual wash and dry with aid of heat

Manual mounting and coverslip after DAPI

Manual dispensing of micro-reagents (proteinase, probe and DAPI)

Special Stains

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Acid Fast Bacteria (AFB) Blue Stain

This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System. The Acid Fast Stain is for demonstration of mycobacteria, especially Mycobacterium tuberculosis, in tissue sections and smears. Mycobacteria are difficult to demonstrate by other staining techniques due to the fatty acid capsule surrounding the organism. This fatty acid covering influences the degree to which stains may penetrate and subsequently be removed. Acid Fast staining may be used for the demonstration of mycobacteria including M. tuberculosis, M. kansasii, M. avium, and M. leprae. Tuberculosis (TB) remains a major health threat, especially in developing countries.

Cat. No. (Xmatrx): SS025-50X

Specificity: Mycobacterium tuberculosis

Expected Results: Red (Acid fast bacilli) and Blue (non-acid fast bacteria)

Lung tissue infected with Mycobacterium tuberculosis

Acid Fast Bacteria (AFB) Green Stain

Mycobacteria are difficult to demonstrate by other staining techniques due to the fatty acid capsule surrounding the organism. This fatty acid covering influences the degree to which stains may penetrate and subsequently be removed. Acid Fast staining may be used for the demonstration of mycobacteria including M. tuberculosis, M. kansasii, M. avium, and M. leprae. Tuberculosis (TB) remains a major health threat, especially in developing countries. A major cause of death in AIDS patients in Africa is TB. M. kansasii and M. avium are also frequently responsible for opportunistic infections in these AIDS patients. Acid Fast remains one of the most common stains used.


specificity: Mycobacterium spp

Expected Results: Acid fast bacilli stains red while the background stains green

Lung tissue infected with Mycobacterium tuberculosis

Alcian Blue/PAS Stain

This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. Alcian Blue PAS is a combined method utilizing the properties of both the PAS and Alcian Blue methods to demonstrate the full complement of tissue proteoglycans. Alcian Blue offers comprehensive staining for acid mucins at pH 2.5. The staining results are insoluble in water or alcohol and do not fade appreciably over time. Neutral mucins which are solely PAS positive will subsequently be demonstrated in a contrasting manner. Where mixtures occur, the resultant colour will depend upon the dominant moiety.


Cat. No. (i6000): SS026-YAK

Specificity: Neutral and acidic mucosubstances

Expected Results: Acid mucins stain blue, neutral mucins stain magenta, mixtures of mucins stain blue/purple; nuclei stain deep blue

Alcian Blue staining of colon tissue

Alcian Blue pH 2.5 Stain

This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. Alcian Blue offers comprehensive staining for acid mucins and stains very intensely over a varying pH range. Strongly sulphated mucins stain below pH 1.0, weakly sulfated mucins stain at pH 2.5 and above, while sialomucins (depending on their type) stain between pH 1.5 and 3.2. Alcian Blue is a water soluble, high molecular weight, copper phthalocyanin basic dye. This Alcian Blue kit stains mucins at pH 2.5. The staining results are insoluble in water or alcohol and do not fade appreciably over time.



Specificity: Acid mucins and mucopoly-saccharides

Expected Results: Acid mucopoly- saccharides stain blue; nuclei stain pink to red; cytoplasm stains pale pinkAlcian blue staining of colon tissue

Alizarin Red Stain

This product is intended for in vitro diagnostic use and is used to identify calcium in tissue sections. Calcium forms an Alizarin Red S-calcium complex in a chelation process and the reaction is birefringent. The reaction is not strictly specific for calcium, since magnesium, manganese, barium, strontium, and iron may interfere, but these elements usually do not occur in sufficient concentration to interfere with the staining.

Cat. No. (Manual): SS052-50K

Specificity: Calcium in tissue section

Expected Results: Calcium deposits stain orange-red

Calcium deposits in bone tissue

Amyloid (Congo Red) Stain

This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. Amyloids are insoluble fibrous protein aggregates that erroneously interact with one another or other cell components forming insoluble fibrils. Abnormal accumulation of amyloid fibrils in organs may lead to amyloidosis and play a role in various neurodegenerative disorders. Amyloid deposits are extracellular and may become sufficiently large to cause damage to surrounding tissues. Congo Red is the gold standard method for diagnosis of amyloidosis. When stained with Congo Red and viewed through polarizing lenses amyloid will birefringe an apple green color under the microscope.



Specificity: Amyloid in tissue sections

Expected Results: Amyloid stains red to deep pink; nuclei stain blue; elastic fibers stain light pink

Amyloid staining of liver tissue

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Azure A Stain

This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. Mast cells are normally present in small numbers in the connective tissue of all organs, but particularly in the dermal layer of skin and are identified by their cytoplasmic granules. Increased numbers of mast cells are found in many pathological conditions. Mast cell hyperplasia in the skin (mastocytosis) manifests with skin lesions and may present with symptoms of urticaria and flushing due to the chemical mediators released during mast cell degranulation.



Specificity: Mast cells in skin tissue (neurofibroma)

Expected Results: Mast cell granules stain purple; Nuclei stain blue; Background stains pale blueAzure A staining of mast cells in

skin tissue

Bielschowsky’s Silver Stain

Bielschowsky’s stain is a silver stain technique used to demonstrate neurofibrillary tangles, nerve fibers and senile plaques in Alzheimer’s disease.



Specificity: Nerve fibers

Expected Results: Axons, neurofibrillary tangles and senile plaques (black); Background (yellow to brown)

Bielschowsky’s staining of brain tissue

Colloidal Iron Stain

This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated System and also for i6000 staining. The Colloidal Iron Stain Kit is used for the demonstration of carboxylated and sulfated mucopoly-saccharides and glycoproteins and is suitable for any well-fixed paraffin embedded tissue cut at 5 microns. Colloidal ferric ions are, at low pH, absorbed principally by carboxylated and sulfated mucosubstances.



Specificity: Carboxylated and sulfated mucopoly- saccharides and glycoproteins

Expected Results: Acid mucopoly- saccharide and sialomucins appear deep blue, nuclei appear pink-red and cytoplasm appears pink

Collodal Iron staining of adeno carcinoma of intestine

Elastic Stain

This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System. The elastic staining procedure can demonstrate the characteristics of emphysema (i.e., atrophy of elastic tissue), arteriosclerosis (i.e., thinning and loss of elastic fibers) and various other vascular diseases.


Specificity: Staining of elastin in tissue sections

Expected Results: Elastic fibers (shades of dark blue); Collagen fibers (shades of red); Nuclei (shades of blue to black); Other tissue elements (shades of yellow)

Elastic fibers in aorta tissue

Giemsa Stain

This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. It is recognized that Helicobacter pylori is the cause of most stomach and duodenal ulcers. Approximately 95% of persons with gastric ulcers and 100% of persons with chronic gastritis have this bacterium within the stomach. The organism has not been found in healthy persons. Those without stomach ulcers or gastritis. H. pylori are known to bind to the O blood-group structure (a particular series of sugars) present in gastric epithelial cells.



Specificity: Helicobacter pylori

Expected Results: Helicobacter pylori and nuclei stain dark blue, and cytoplasm stains pink

Giemsa stain for H.pylori in tissue sections

Gomori’s Trichrome Blue Stain

This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. Trichrome stains have historically been used to distinguish collagen from muscle tissue. The term “trichrome” refers to a mix of three stains. These dyes stain nucleus, collagen and cytoplasm structures and are often in mordents such as phosphotungstic or phosphomolybdic acid. Use of trichrome stain may be useful in the study of diseases of connective tissue and muscle characterized by fibrotic and dystrophic changes and to differentiate between collagen and smooth muscle in tumors.



Specificity: Muscle and collagen fibers

Expected Results: Muscle fibers stain red; Collagen stains blue; Nuclei stain blue-black

Gomoris Trichrome staining of Fallopian tube

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Gomori’s Trichrome Green Stain

This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. Trichrome stains have historically been used to distinguish collagen from muscle tissue. The term “trichrome” refers to a mix of three stains. These dyes stain nucleus, collagen and cytoplasm structures and are often in mordants such as phosphotungstic or phosphomolybdic acid. Use of trichrome stain may be useful in the study of diseases of connective tissue and muscle characterized by fibrotic and dystrophic changes and to differentiate between collagen and smooth muscle in tumors.




Expected Results: Nuclei (blue), Collagen (green), Muscle Fiber (red)

Gomori’s Trichrome staining of Fallopian tube

Gram Stain

This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. The Gram Stain procedure continues to be one of the initial screening tests for detecting bacteria in wounds, sputum, pus, tissues, etc. Although the exact chemical nature of the method is yet to be established, it is known that Gram positive organisms, staining a deep blue color, have a cell wall containing teichoic acid, while Gram negative organisms, staining a red-pink color, have cell walls containing lipopolysaccharides.



Specificity: Gram positive and Gram negative microorganisms

Expected Results: Gram positive- (blue), Gram- negative (pink to red)

Gram staining of gram-negative bacilli

Grocott’s Methenamine Silver (GMS) for Fungi Stain

This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. Human bodies are regularly exposed to fungi spores from many species. Patients with a diminished or compromised immune system show greater susceptibility and incidence of fungal infections. Some fungi may elicit a range of host reactions from oxidative, necrotising to granulomatous. Other fungi produce little cellular response to indicate their presence. Most fungi are relatively large and their cell walls are rich in polysaccharide. These polysaccharides can be oxidized to di-aldehydes and detected with Schiff’s reagent or silver solution.



Specificity: Fungi in tissue sections

Expected Results: Fungi stain gray to black with a light green background

GMS staining for fungi in tissue sections

Iron Stain

This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. Small amounts of ferric iron may be found in bone marrow and spleen. Very large deposits may be seen in conditions such as hemachromatosis and hemosiderosis.



Specificity: Detection of ferric iron in tissue sections, and blood or bone marrow films

Expected Results: Iron (bright blue); Nuclei (red); Cytoplasm (pink)

Spleen tissue with iron deposits

Jones’ Basement Membrane Stain

This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated System and also for i6000 staining. Hematoxylin and Eosin stained sections of most tissue do not distinguish between basement membranes well. In disorders such as membranous nephropathy or diabetes the basement membranes in the glomerulus become more conspicuous. Jones’ Basement Membrane stain kit is used to identify basement membranes, specifically glomerular and tubular membranes in renal tissue.



Specificity: Basement membrane

Expected Results: Basement membrane (black); Nuclei (red); Background (pink)

Jones’ basement membrane staining of kidney with glomeri

Masson’s Trichrome Stain

This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. Trichrome stains have historically been used to distinguish collagen from muscle tissue. The term “trichrome” refers to a mix of three stains. These dyes often stain nucleus, collagen and cytoplasm structures in mordants such as phosphotungstic or phosphomolybdic acid. Use of Masson’s trichrome stain may be useful in the study of diseases of connective tissue and muscle characterized by fibrotic and dystrophic changes and to differentiate between collagen and smooth muscle in tumors.




Expected Results: Collagen (blue); Nuclei (black); Muscle, cytoplasm, keratin (red)

Masson’s Trichrome staining of stomach tissue

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Mucicarmine Stain

This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. Mucicarmine preferentially stains mucin with strong staining of epithelial mucins and poor staining of mucins of fibroblastic origin. Staining results may be used to identify primary tumor sites, distinguishing mucin-negative undifferentiated squamous cell lesions from mucins-positive adenocarcinomas and staining capsule of Cryptococcus.



Specificity: Mucopoly- saccharides

Expected Results: Mucins (deep rose); Nuclei (black); Other tissue elements (yellow)

Mucicarmine staining of small intestine

Oil Red O Stain

This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. Oil Red O stain is an oil soluble dye used to evaluate normal or abnormal fatty tissue. Abnormal deposits of fatty emboli may develop after a bone fracture or an injury that crushes fatty tissue.



Specificity: Fat staining on frozen sections

Expected Results: Fat (red); Nuclei (blue)

Oil Red O staining of fat containing tissue

Periodic Acid-Schiff (PAS) Diastase Stain

This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. PAS is a stain that typically gives a magenta color in the presence of glycogen. Diastase is an enzyme that breaks down glycogen into smaller sugar units, maltose and glucose that are washed out of the section. Differences in the intensities of the two stains (PAS and PAS-D) can be attributed to different glycogen concentrations and can be used to semi-quantify glycogen in samples.



Specificity: PAS stains specifically glycogen, glycoproteins, mucopoly- saccharides, basement membrane and mucin.

Expected Results: Nuclei stain blue; glycogen and other carbohydrates stain red to pink; No stained glycogen seen in diastase-digested tissue

PAS Diastase staining of liver tissue

Periodic Acid-Schiff (PAS) for Fungi Stain

This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. PAS for fungi is a staining method used to identify fungi in formalin-fixed and paraffin-embedded tissues or autopsy tissues. Polysaccharides present in fungal cell walls are oxidized by the periodic acid to aldehydes. The aldehydes react with the Schiff reagent to yield rose-colored staining of fungi.



Specificity: PAS for Fungi is specific for fungal organisms in tissue sections

Expected Results: Fungi stain rose and background stains green to blue

PAS staining of fungi in lung tissue

Periodic Acid-Schiff (PAS) Stain (without Diastase)*

This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. The PAS reaction in tissue sections is useful for outlining tissue structures such as basement membranes, capsules and blood vessels. This staining procedure may also be used for the demonstration of fungal organisms in tissue sections. PAS staining is mainly used for staining structures containing a high proportion of carbohydrate macromolecules (glycogen, glycoprotein, proteoglycans) typically found in connective tissues, mucus, the glycocalyx, and basal laminae.



Specificity: Glycogen glycoproteins; Mucopoly- saccharides; Basement membrane and mucin

Expected Results: Glycogen (red-purple); Background (blue)

PAS staining of liver tissue

Renal Masson’s Trichrome Stain

Trichrome stains have historically been used to distinguish collagen from muscle tissue. The term “trichrome” refers to a mix of three stains. These dyes often stain nucleus, collagen and cytoplasm structures in mordants such as phosphotungstic or phosphomolybdic acid.



Specificity: Collagen

Expected Results: Fibrinoid and immune deposits appear red; Basement membrane & collagen stain green and nuclei stain blueRenal Masson’s Trichrome staining

of kidney


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Reticulin/No Counterstain

This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. Ammonical silver stains are common methods for the demonstration of reticular fibers. These procedures may be used for the differential diagnosis of tumors such as carcinomas, sarcomas and lymphosarcomas.



Specificity: Reticular fibers

Expected Results: Reticulin stains black without any counter stain

Reticulin staining of liver tissue

Reticulin/Nuclear Fast Red Stain

Ammonical silver stains are common methods for demonstration of reticular fibers. These procedures may be used for the differential diagnosis of tumors such as carcinomas, sarcomas and lymphosarcomas.



Specificity: Reticular fibers

Expected Results: Reticulin stains black with a pink to rose background

Reticulin staining of kidney tissue

Safranin O Stain

This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. Safranin O staining is used for the detection of cartilage, mucin, and mast cell granules on formalin-fixed paraffin-embedded and frozen tissue sections. In this staining, cartilage and mucin stain orange to red, nuclei stain black and the background is stained gray green. Safranin O is used to demonstrate any changes that occur in articular diseases.



Specificity: Cartilage, mucin, and mast cell granules

Expected Results: Nuclei stain black, cytoplasm stains gray-green green, cartilage, mucin & mast cell granules stain orange to redSafranin O staining of cartilage

Sudan Black B Stain*

This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. Sudan Black B is a classical dye used for studying lipids in tissue sections. Sudan black B stain is used to differentiate fat cell tumors (liposarcomas) from other types of tumors. Abnormal deposits of fatty emboli may develop after a bone fracture or an injury that crushes fatty tissue. The fat stain may verify that an emboli caused death. When cell membranes or myelin degenerate, fatty substances may be formed and can be detected with this stain.



Specificity: Sudan Black B staining procedure is used to demonstrate neutral lipids and phospholipids in frozen tissue sections

Expected Results: Fat stains blue-black and nuclei stain red

Fat in frozen sections stained by Sudan black B

Toluidine Blue Stain

This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System. Mast cells normally present in the connective tissue of all organs are increased in many pathological conditions. Mast cell granules which are refractile and not readily identified in H&E stained sections are well demonstrated by the Toluidine blue staining method.



Specificity: Mast cell

Expected Results: Mast cell granules and carboxylated mucins (purple); Nuclei (blue); Background (pale blue)

Toluidine blue staining of mast cells in skin tissue

Van Gieson Stain

This product is intended for in vitro diagnostic use on the BioGenex Xmatrx Automated Staining System and also for i6000 staining. Van Gieson Stain is used to differentiate between collagen and smooth muscle in tumors and to demonstrate the increase of collagen in diseases. This method combines two or more anionic dyes and relies on differential binding by tissue components. The differentiation is determined by a combination of differences in the relative size of the dye molecules, differences in the physical structure of the tissue, and differences in the amino acid composition of tissue elements.



Specificity: Collagen and smooth muscle in tumors

Expected Results: Nuclei (blue/black); Collagen (red); Cytoplasm, muscle fibrin and red blood cells (yellow)

Van Gieson staining of stomach tissue


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Special Stains (Xmatrx®)Product Name Pack size Cat. No.

Acid-Fast Bacteria (AFB) Blue Stain 50 Tests SS025-50X

Acid-Fast Bacteria (AFB) Green Stain 50 Tests SS059-50X

Alcian Blue/PAS Stain 50 Tests SS026-50X

Alcian Blue pH 2.5 Stain 50 Tests SS027-50X

Amyloid (Congo Red) Stain 50 Tests SS028-50X

Azure A Stain 50 Tests SS038-50X

Bielschowsky’s Silver Stain* 50 Tests SS055-50X

Colloidal Iron Stain 50 Tests SS054-50X

Elastic Stain 50 Tests SS029-50X

Giemsa Stain 50 Tests SS049-50X

Gomori's Trichrome Blue Stain 50 Tests SS033-50X

Gomori's Trichrome Green Stain 50 Tests SS034-50X

Gram Stain 50 Tests SS037-50X

Grocott's Methenamine Silver (GMS) Stain 50 Tests SS042-50X

Iron Stain 50 Tests SS030-50X

Jones’ Basement Membrane Stain* 50 Tests SS058-50X

Masson's Trichrome Stain 50 Tests SS035-50X

Mucicarmine Stain 50 Tests SS036-50X

Oil Red O Stain* 50 Tests SS043-50X

Periodic Acid-Schiff (PAS) Diastase Stain 50 Tests SS039-50X

Periodic Acid-Schiff (PAS) for Fungi Stain 50 Tests SS053-50X

Periodic Acid-Schiff (PAS) Stain (without Diastase)* 50 Tests SS032-50X

Renal Masson’s Trichome Stain 50 Tests SS050-50X

Reticulin/No Counterstain 50 Tests SS046-50X

Reticulin/Nuclear Fast Red Stain 50 Tests SS047-50X

Safranin O Stain 50 Tests SS040-50X

Sudan Black B Stain* 50 Tests SS041-50X

Toluidine Blue Stain 50 Tests SS057-50X

Van Gieson Stain 50 Tests SS044-50X* To be released soon

Von Kossa Stain

This product is intended for in vitro diagnostic use to demonstrate deposits of calcium in tissues. Abnormal deposits of calcium may be found in any area of the body. With the H&E stain, calcium appear deep blue-purple. Von Kossa staining is specific for demonstrating deposits of calcium or calcium salt and is not specific for the calcium ion itself.When tissue sections are treated with a silver nitrate solution, the silver is deposited by replacing the calcium, reduced by the strong light, and thereby visualized as metallic silver. Additional methods are sometimes employed along with this technique to confirm the presence of calcium such as Alizarin Red, which detects calcium deposits.

Cat. No. (Manual): SS045-50K

Specificity: Calcium salts in tissue sections

Expected Results: Calcium salts (black or brown black); Nuclei (red) & cytoplasm (pink)

Von Kossa staining of calcified node

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Alcian Blue/PAS Stain 50 Tests SS026-YAK

Alcian Blue pH 2.5 Stain 50 Tests SS027-YAK

Amyloid (Congo Red) Stain 50 Tests SS028-YAK

Azure A Stain 50 Tests SS038-YAK

Bielschowsky’s Silver Stain* 50 Tests SS055-YAK

Colloidal Iron Stain 50 Tests SS054-YAK

Giemsa Stain 50 Tests SS049-YAK

Gomori's Trichrome Blue Stain 50 Tests SS033-YAK

Gomori's Trichrome Green Stain 50 Tests SS034-YAK

Gram Stain 50 Tests SS037-YAK

Grocott's Methenamine Silver (GMS) for Fungi Stain 50 Tests SS042-YAK

Iron Stain 50 Tests SS030-YAK

Jones’ Basement Membrane Stain* 50 Tests SS058-YAK

Masson's Trichrome Stain 50 Tests SS035-YAK

Mucicarmine Stain 50 Tests SS036-YAK

Oil Red O Stain* 50 Tests SS043-YAK

Periodic Acid-Schiff (PAS) Diastase Stain 50 Tests SS039-YAK

Periodic Acid-Schiff (PAS) for Fungi Stain 50 Tests SS053-YAK

Periodic Acid-Schiff (PAS) Stain (without Diastase)* 50 Tests SS032 -YAK

Renal Masson's Trichrome Stain 50 Tests SS050-YAK

Reticulin/No Counterstain 50 Tests SS046-YAK

Reticulin/Nuclear Fast Red Stain 50 Tests SS047-YAK

Safranin O Stain 50 Tests SS040-YAK

Sudan Black B Stain* 50 Tests SS041-YAK

Toluidine Blue Stain 50 Tests SS057-YAK

Van Gieson Stain 50 Tests SS044-YAK

Special Stains (i6000TM)


Alizarin Red Stain 50 Tests SS052-50K

Von Kossa Stain 50 Tests SS045-50K

Special Stains (Manual)


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Ancillary Reagents

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Ancillary Reagents

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Ancillary Reagents

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Buffers and DiluentsBuffers and diluents are available for most applications in Immunohistochemistry and in situ Hybridization. General buffers, such as PBS and TBS can be used for washing/rinsing of slides.

BioGenex offers several diluents for specific antibody or reagent products. The Common Antibody Diluent and Enhanced Antibody Diluent have been developed for use with all antibodies in immunohistochemistry and have been specifically optimized for use with BioGenex antibodies and reagent products. These diluents enable enhancement of signal-to-noise ratio of staining when used optimally and also help in maintaining the antibody specificity and stability. The Enhanced Antibody Diluent, in addition to all the above features, contains chemical compounds to enhance antigen-antibody interaction and affinity.

In order to achieve desired staining pattern and intensity, the titers of antibodies or concentrations of reagents may need to be optimized.

Buffers - GeneralProduct Name Pack size Cat. No.

Phosphate Buffered Saline, 25X Concentrate (pH 7.6 upon dilution; makes 3L total)

120 ml HK091-5KE

Tris Buffer, dried powder (wash buffer) (pH 7.6, 0.1M upon reconstitution; makes 3L total)

3 packets HK098-5K

Buffers - WashProduct Name Pack size Cat. No.

Super SensitiveTM Wash Buffer (20X concentrated) 500 ml HK583-5KE

DiluentProduct Name Pack size Cat. No.

Link Diluent (not for use with concentrated Goat Link, Cat. No. HK327-UG)

100 ml HK165-5KE

Streptavidin Peroxidase Diluent (use with concentrated peroxidase labels) 100 ml HK157-5KE

Common Antibody Diluent (Contains Sodium Azide) 100 ml HK156-5KE

Enhanced Antibody Diluent (RTU) 100 ml HK941-YAKE

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Avidin/Biotin Blocking Kit: Certain tissues especially liver, kidney, and GI tract are rich in biotin. Use of the Avidin/Biotin Blocking kits ensures the blocking of all endogenous biotin, biotin receptors, or avidin binding sites present in the tissue. Pre-treatment of tissues with avidin blocking should always be followed with biotin blocking.

Peroxide Block: Endogenous peroxidase is most commonly encountered in red blood cells, kidney, and liver tissue. Peroxide Block should be used prior to application of primary antibody when Horseradish Peroxidase (HRP) is used as the labeling enzyme, and if it is necessary to block endogenous peroxidase activity in the tissue being stained.

Power BlockTM: This is a blocking reagent for reducing nonspecific background in immunoassays. A truly universal block, it is suitable for use in immunohistochemistry, immunocytochemistry, ELISA methods, and immunogold techniques. The Power BlockTM reagent contains buffer, casein and preservative and also works well as an antibody diluent and washing medium.

Protein Block: This can be used to reduce background staining due to non-specific binding of the primary or secondary antibodies to the tissue. Protein Block should be applied immediately prior to the primary antibody.

Levamisole: This is used to reduce background staining of alkaline phosphatase-based detection systems by inhibiting endogenous alkaline phosphatase in most tissues. It is intended for use with detection systems that employ intestinal alkaline phosphatase as the enzyme label. Levamisole is added to an alkaline phosphatase chromogen (Fast Red or BCIP/NBT).

Blocking Reagents


Avidin/Biotin Blocking Kit (RTU) 60 slides HK102-5K

Peroxide Block 6 ml HK111-5K

Peroxide Block 50 ml HK111-50K

Power BlockTM Universal Blocking Reagent (10X concentrated) 100 ml HK085-5KE

Power BlockTM Universal Blocking Reagent (Ready-to-use) 25 ml BS-1310-25

Protein Block / Normal Goat Serum (for use with Mouse & Rabbit Link and Detection Systems), including MultiLink®

6 ml HK112-5K

Protein Block / Normal Goat Serum (for use with Mouse & Rabbit Link and Detection Systems), including MultiLink®

50 ml HK112-9KE

Protein Block/Normal Rabbit Serum (for use with Goat and Rat link and Detection System)

6 ml HK114-5K

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a AEC, 3-amino-9-ethylcarbazoleb DAB, 3,3'-diaminobenzidine tetrahydrochloridec Ideal for use with product QD440-XAK (please refer to page 139)

Substrates and ChromogensBioGenex offers complete Substrate Packs for immunohistochemical staining with alkaline phosphatase and peroxidase labels. The kits are designed to reduce substrate preparation time and minimize exposure to chemical hazards.

In the subsequent section that covers counterstains and mounting media, a chart has been provided that summarizes the substrates offered, indicating enzyme and standard mounting media compatibility.

BioGenex also offers "One-Step" AEC Solutions for staining convenience. The solution is ready-to-use and eliminates the need for any reagent mixing. This product provides crisp and clear staining patterns and allows excellent visualization of cellular components. Color development is rapid, i.e., 5 minutes or less in most cases.

Features• High Resolution AEC and Liquid DAB• Rapid Development Time• Ready-to-use Solutions• Long-Term Stability


AECa (For Aqueous Mounting)

AEC One Step Solution (Recommended for Manual Use) 6 ml HK139-06K

AEC One Step Solution (Recommended for use in Automation) 50 ml HK139-50KE

AEC Substrate Pack (AEC chromogen 3 ml, H2O2 Substrate/Buffer 11 x 2.5 ml)

250 slides HK092-5K

AEC Substrate Pack, Concentrated (AEC chromogen 2 x 6 ml, Concentrated H2O2 Substrate Buffer 3 x 17.5 ml)

2500 slides HK129-5KE

DABb (For Permanent Mounting)

Super Sensitive™ DAB (RTU) (Liquid DAB 5 ml, Stable Substrate Buffer 100 ml)

1000 slides HK542-XAKc

Liquid DAB chromogen 4 ml HK124-7K

Substrate Packs for immunohistochemical staining with peroxidase

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Substrates and Chromogens

Elegance Red (For Permanent Mounting)Product Name Pack size Cat. No.

Elegance Red Substrate Pack (Naphthol Phosphate Substrate, Permanent Red activator, Permanent Red Chromogen, Tris Buffer)


Fast Red (For Aqueous Mounting)Product Name Pack size Cat. No.

Fast Red Substrate Pack 11 Fast Red tablets, 11 x 5 ml Naphthol Phosphate Buffer


Naphthol Phosphate Substrate (for use with Fast Red Tablets)

15 ml HK180-7K

New Fuchsin (For Permanent Mounting)Product Name Pack size Cat. No.

New Fuchsin Substrate Pack (2.5 ml. New Fuchsin Chromogen, 2.5 ml New Fuchsin Activator, 5 ml of New Fuchsin Substrate, 8 x 5 ml New Fuchsin Tris Buffer)


Substrate Packs for Immunohistochemical Staining with Alkaline Phosphatase

Negative Control Sera/Immunoglobulins Each staining run should include a negative control slide to confirm reagent specificity. BioGenex, for this purpose, offers negative control sera/immunoglobulins which have been optimized for use as negative controls for our Super SensitiveTM, Ready-to-use antibodies. Negative controls are available for Mouse, Rabbit, Goat and Rat antibodies.


Mouse, Super SensitiveTM Negative Control - gammaglobulin 17 ml HK119-7M

Goat, Super SensitiveTM Negative Control 3 ml HK406-5G

Rat, Super SensitiveTM Negative Control 3 ml HK407-5T

Rabbit, Super SensitiveTM Negative Control 3 ml HK408-5R

Rabbit, Super SensitiveTM Negative Control 17 ml HK408-7R

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Chromogens Stain Color Solubility

AEC Brick Red Alcohol/Xylene Soluble

BCIP/NBT Blue-Purple Alcohol/Xylene Insoluble

DAB Brown Alcohol/Xylene Insoluble

Elegance Red Red Alcohol/Xylene Insoluble

Fast Red Red Alcohol/Xylene Soluble

New Fuchsin Red Alcohol/Xylene Soluble

Counterstains Stain Color Solubility Pack size Cat. No.

Hematoxylin, Mayer's (Non-alcohol-based) Blue Alcohol/Xylene Insoluble 6 ml HK100-5K*

Hematoxylin, Mayer's (Non-alcohol-based) Blue Alcohol/Xylene Insoluble 250 ml HK100-9K*

Mounting Media Solubility Pack size Cat. No.

Aqueous Mounting Medium Uses aqueous solution 15 ml HK099-5K

SuperMount® Permanent Mounting Medium Uses aqueous solution 15 ml HK079-5K

SuperMount® Permanent Mounting Medium Uses aqueous solution 50 ml HK079-7K

BioGenex offers a selection of counterstains and mounting media for use in Immunohistochemistry and in situ Hybridization. In IHC and ISH procedures, a histological or cytological specimen affixed to a microscope slide is stained with a chromogen, counterstained, and mounted. The mounting medium may be used to attach a coverslip or may itself serve as a coverslip substitute. The choice of mounting medium depends on whether long-term ("permanent" mounting) or short-term preservation ("aqueous" mounting) is desired, and whether the mounting procedure is chemically compatible with the chromogen and the counterstain.

BioGenex offers a unique SuperMount® Permanent Aqueous Mounting Medium. This is a polymer based mounting media that does not require the use of a coverslip. This innovative, patented mounting medium (BioGenex's U.S. Patent No. 5,492,837) is designed to preserve biological specimens for long-term storage. SuperMount® medium is compatible with most aqueous and organic-soluble dyes and chromogens including AEC, DAB, Elegance Red, Fast Red, New Fuchsin, BCIP/NBT, Rhodamine, Fluorescein, Texas Red, Phycoerythrin, Phycocyanin, and Fat Stain (Oil Red O). The refractive index of SuperMount® yields greater transparency and clarity of specimens to be examined under the microscope. When applied in a thin coat and allowed to harden, SuperMount® medium forms a highly transparent coating that permanently preserves stained tissue sections. SuperMount® can be used for the mounting of all biological specimens, including stained tissue sections, Cytospin preparations, and blood smears. BioGenex also offers Aqueous Mounting Medium, which on the other hand, is glycerol-based and does require the use of a coverslip.

The compatibility chart on the next page summarizes the recommended mounting medium for different combinations of chromogen and counterstain.

Counterstains and Mounting Media

* For in vitro Diagnostic Use.

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* For in vitro Diagnostic Use.

Compatible Mounting Media for Chromogens and Counterstains

Counterstains AEC BCIP/NBT DAB Elegance Red Fast Red New Fuchsin

Hematoxylin Aqueous/ SuperMount®

Aqueous /Not recommended

Aqueous/ Permanent/ SuperMount®


SuperMount® /

AqueousSuperMount®

Methyl Green Not recommendeda

Permanent Permanent Permanent Permanent Permanent

Nuclear Fast Red

Not recommendeda



Not recommendeda Not recommendeda

Not recommendeda

a Not Recommended (may have poor color based contrast)

Microscope Slides and AccessoriesOptiPlusTM Positive-Charged Microscope Slides provide a strong adhesive surface for tissues and cells to prevent tissue displacement during harsh pre-treatments such as enzymatic digestion and the microwave Antigen Retrieval method. These slides are ideal for automated systems. Additionally, each slide has a frosted end for easy labeling.

The OptiPlusTM Positive-Charged Barrier Slides have all the advantages of our regular OptiPlusTM slides, but also contain hydrophobic barriers that allow the quantity of reagents per slide to be tailored to the size of the specimen. These slides eliminate reagent waste without the need to use a PAP pen, thereby reducing set-up time in manual assays as well as in automated systems. The slides come in three different configurations to accommodate different tissue sizes or multiple tissues per slide:

1. A single, full-size test area of 25 x 40 mm

2. A single 2/3-size test area of 25 x 30 mm, and

3. Three 1/3-size test areas per slide, each compartment measuring 25 x 15 mm

For accessories to automated instruments please refer to Automation System section of the Catalog.


PAP Pen 1 each XT001-PP

OptiPlusTM Positive-Charged Microscope Slides 72 slides/box XT002-SL

OptiPlusTM Positive-Charged Microscope Slides 20 boxes/case XT002-CL

OptiPlusTM Positive-Charged Barrier Slides (full test area) 72 slides/box XT134-SL

OptiPlusTM Positive-Charged Barrier Slides (full test area) 20 boxes/case XT134-CL

OptiPlusTM Positive-Charged Barrier Slides (2/3 test area) 72 slides/box XT013-SL

OptiPlusTM Positive-Charged Barrier Slides (2/3 test area) 20 boxes/case XT013-CL

OptiPlusTM Positive-Charged Barrier Slides (3 x 1/3 test areas) 72 slides/box XT014-SL

OptiPlusTM Positive-Charged Barrier Slides (3 x 1/3 test areas) 20 boxes/case XT014-CL

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Tissue Control

PR014Kappa123456Dr. James Smith

John Black12/2/2009

BioGenex

PR014Kappa123456Dr. James Smith

John Black12/2/2009

BioGenex

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Tissue Control

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Positive Control Slides and Barrier SlidesPositive control slides are made with tissue which has undergone processing identical to that of the test tissue. BioGenex provides positive control slides that enable one to confirm antibody reactivity.

Barrier slides are positive control tissue slides with barriers to prevent loss of reagent.

Pack size: Positive Control slides (5 slides per pack) Barrier slides (5 slides per pack)

Antibody Clone Recommended Positive Control Control Slides Cat. No

Barrier Slides Cat. No

Aberrant Endothelial Cell 4A11 Tonsil / Lymph Node NA NA

ACTH AH26 Pituitary FG-487N NA

Actin, Muscle-Specific HHF35 Heart / Skeleton Muscle FG-090M FB-090M

Actin, Smooth Muscle 1A4 Colon FG-128M FB-128M

Adenovirus A62020069P Adenovirus Infected Cells/Tissues FG-059M NA

Alpha-1-Antichymotrypsin α1A88 Liver Carcinoma NA NA

Alpha-1-Antitrypsin Polyclonal Hepatocellular Carcinoma FG-015P NA

Alpha-Actinin JLN20 Muscle FG-097M NA

Alpha-Fetoprotein (AFP) C3 Hepatocellular Carcinoma FG-008M FB-008M

Alpha-Tubulin DM-1A Skin/Lung FG-121M NA

Androgen Receptor F39.4.1 Prostate/Prostate Carcinoma FG-256M FB-256M

Apolipoprotein E EP1373Y Liver FG-567N NA

B Cell MB2 Tonsil FG-158M NA

B Lyphocyte Antigen 36 (BLA.36) A27-42 B Lyphocytes/Hodgkin FG-231M FB-231M

Bax Protein Polyclonal Breast Carcinoma NA NA

Bcl-2 E17Follicular Lymphoma and Reactive Lymph node Tissues

FG-541N NA

Bcl-2 EP36 Tonsil FG-723N FB-723N

Bcl-2 Oncoprotein Bcl-2/100 Tonsil FG-287M FB-287M

Bcl-6 LN22 Tonsil FG-708M FB-708M

Bcl-x E18 Breast Carcinoma FG-462N NA

Beta-Catenin E247 Colon Carcinoma FG-510N NA

Beta-Tubulin DM-1B Skin/Lung FG-122M NA

Beta-Tubulin II JDR3B8 Small Intestine/ Colon FG-176M NA

Beta-Tubulin III SDL3D10 Heart FG-177M NA

Beta-Tubulin IV ONS1A6 Lung FG-178M NA

bFGF (Basic Fibroblast Growth Factor) bFGF88 Adenocarcinoma NA NA

Blood Group Antigen Lewis A 7LE Stomach FG-303M NA

Blood Group Antigen Lewis B 2-25LE Stomach FG-304M NA

BOB-1 SP92 Tonsil FG731-N FB-731N

BRCA1 Protein Polyclonal Hepatocellular Carcinoma NA NA

Breast Cancer Antigen BCA 225 CU18 Breast Carcinoma FG-135M NA

CA19-9 C241:5:1:4 Colon FG-424M FB-424M

CA 125 (Ovarian Tumor Marker) Ov185:1 Ovary Carcinoma FG-429M FB-429M

Caldesmon E89 Prostate FG-507N NA

Caldesmon,High MW,Smooth muscle h-CD Breast Carcinoma NA NA

Calponin CALP Breast Carcinoma FG-333M NA

Calponin-1 (C-term) EP798Y Pleomorphic Adenoma FG-502N NA

Calretinin 2E7 Brain Tissue/Appendix FG-583M NA

Calretinin Polyclonal Brain FG-413P NA

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Carcinoembryonic Antigen (CEA) B01-94-11M-P Normal Colon/ Colon Carcinoma FG-009M NA

Carcinoembryonic Antigen (CEA) CEA88 Bowel Carcinoma FG-365M FB-365M

Carcinoembryonic Antigen (CEA) Polyclonal Bowel/Colon Carcinoma FG-009P NA

Catenin (p120) YE372 Breast Carcinoma FG-523N NA

Catenin delta 1(p120) Polyclonal Breast Carcinoma FG-706P FB-706P

Cathepsin D C15 Breast Carcinoma NA NA

CD1a O10 Lymph Node FG-490M NA

CD2 AB75 Lymph Node/Tonsil FG-438M NA

CD3 Epsilon EP449E Spleen FG-477N NA

CD3 (T Cell) UCHT1 Tonsil NA NA

CD3 (T Cell) PS1 Tonsil FG-322M FB-322M

CD3 and CD20 cocktail EP449E and L-26 Spleen/Tonsil FG-559C NA

CD4 4B12 Tonsil FG-421M FB-421M

CD4 EP204 Tonsil/T-cell Lymphoma FG-722N FB-722N

CD4 + C8 Cocktail 4B12+EP1150Y Tonsil NA NA

CD5 4C7 Tonsil FG-430M NA

CD5 EP2952 Lymph Node FG-512N NA

CD7 LP15 Tonsil FG-702M FB-702M

CD8 1A5 Tonsil FG-422M FB-422M

CD8 SP16 Tonsil NA NA

CD8 (C-Term) EP1150Y Lymph Node FG-513N NA

CD8 (T Cell,Suppressor/Cytotoxic) T8 Tonsil NA NA

CD10 56C6 Lymphoma FG-451M NA

CD10 and Cyclin D1 cocktail 56C6 and EPR2241 Breast Carcinoma/Lymphoma FG-561C NA

CD11b (C3bi Receptor) M01 Tonsil NA NA

CD11b/ITAM EPR1344 Spleen FG-546N NA

CD11C EP1347Y Tonsil Tissue FG-555N NA

CD14 EPR3653 Placenta FG-515N NA

CD15 (Blood group antigen Lewis X) BRA4F1 Bone Marrow/Hodgkin FG-302M FB-302M

CD16 2H7 Lymph Node/Breast FG-437M NA

CD19 EP169 Tonsil FG-729N FB-729N

CD20 CD20/C23 Spleen/Tonsil FG-537M NA

CD20 (B Cell) L-26 Tonsil FG-238M FB-238M

CD20 (B Cell) B1 Tonsil NA NA

CD21 EP3093 Tonsil FG-497N NA

CD21 (B Cell) B2 Tonsil/Lymph Node NA NA

CD22 FPC1 Tonsil FG-439M NA

CD23 Polyclonal Tonsil FG-460P NA

CD25 4C9 Tonsil FG-453M NA

CD29 JB1a Breast FG-298M NA

CD30 (Ki-1 Antigen) Ber-H2 Hodgkin’s Lymphoma FG-327M NA

CD30 (Ki-1 Antigen) HRS-4 Hodgkin’s Lymphoma NA NA

CD31 (Endothelial Cell) JC/70A Colon Carcinoma FG-232M NA

CD31 (PECAM-1) 9G11 Lymph Node FG-241M NA

CD34 (Endothelial Cell) QBend/10 Adenocarcinoma lung FG-236M FB-236M

CD35 RLB25 Tonsil FG-431M NA

CD35 SP191 Tonsil NA NA

CD41 Integrin/Alpha IIb EP178 Spleen FG-732N FB-732N

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CD43 & CD45RA Cocktail MT1 &MB1 Tonsil FG-159M NA

CD43 (T Cell,Leukosialin) DFT-1 Tonsil FG-305M NA

CD44 (Phagocytic Glycoprotein-1,HCAM) DF1485 Tonsil FG-310M NA

CD45 Cocktail (Leukocyte Antigen, LCA) MEM55 + LJ27.9 Tonsil FG-371M FB-371M

CD45 (Leukocyte common Antigen,LCA) PD7/26/16&2B11 Tonsil FG-111M NA

CD45 (Leukocyte common Antigen,LCA) LJ27.9 Tonsil NA NA

CD45RA (B Cell) MB1 Tonsil FG-157M FB-157M

CD45RB MEM55 Tonsil FG-320M NA

CD45RC (T Cell) MT2 Tonsil FG-156M FB-156M

CD45RO (T Cell) UCHL-1 Tonsil FG-113M FB-113M



CD56 (Natural Killer Cell,NCAM) NKH-1 Tonsil NA NA

CD57 (Natural Killer Cell) NK-1 Tonsil FG-314M NA

CD61 EP2417Y Kidney FG-482N NA

CD63 EP211 Melanoma/Prostate Ca. FG-720N FB-720N

CD66 BY114 Tonsil FG-325M NA

CD68 KP1 Stomach/Lymph Node FG-416M NA

CD68 CD68/G2 Histiocytoma FG-549M NA

CD71 (Transferrin Receptor) T9 Tonsil NA NA

CD71 (Transferrin Receptor) H68.4 Bone Marrow/Fetal Liver NA NA

CD74 (B Cell) LN2 Tonsil FG-153M NA

CD79a 11E 3 Lymph Node/Tonsil FG-414M NA

CD79a EP82 Tonsil/B-cell Lymphoma FG-719N FB-719N

CD90 EP56 Thymus FG-733N FB-733N

CD95 EP208 Tonsil NA NA

CD99 EPR3097Y Ewings Sarcoma FG-518N NA

CD99 (MIC2 Antigen) HO36.1.1 Thymus/Tonsil NA NA

CD103 EP206 Colon FG-739N FB-739N

CD105 4G11 Uterus FG-441M NA

CD117 T595Gastrointestinal Stromal Tumor/ Stomach

FG-423M NA

CD117 YR145Gastrointestinal Stromal Tumor/ Stomach

FG-465N NA

CD146 EP54 Placenta FG-716N FB-716N


CD227 (MUCIN 1) VU-4H5 Colon/Mucinous Adenocarcinoma FG-534M NA

CDw75 (B Cell) LN1 Tonsil FG-152M NA

CDX-2 CDX2-88 Colon FG-392M NA

CDX-2+CK20+CK7 cocktailCDX2-88+EPR1622Y+EPR1619Y

Colon/Breast Carcinoma NA NA

CDX-2+CK7 cocktail CDX2-88+EPR1619Y Colon/Breast Carcinoma FG-596C FB-596C

c-erbB-2 (HER-2/neu) EP1045Y Breast Carcinoma FG-471N NA

c-erbB-2 (HER-2/neu) CB11 Breast Carcinoma FG-134M FB-134M

c-erbB-2 (HER-2/neu) EP3 Breast Ca. FG-726N FB-726N

c-erbB-3 (HER-3) RTJ1/A2 Breast Carcinoma FG-319M NA

Chromogranin A LK2H10 Pancreas FG-126M NA

187

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Chromogranin A PHE-5 Pancreas FG-356M FB-356M

Claudin 1 Polyclonal Breast Carcinoma/Spleen FG-704P FB-704P

Claudin-5 EP224 Vascular/Lung Squamous Ca. FG-718N FB-718N

c-myc Protein 9E 10 Adenocarcinoma FG-318M NA

Collagen III HWD1.1 Skin FG-167M NA

Collagen IV COL-94 Skin NA NA

Cyclin D1 Polyclonal Breast Carcinoma FG-447P NA

Cyclin D1 EPR2241(IHC)-32 Thyroid Carcinoma FG-474N NA

Cyclin E2 EP454Y Glioblastoma FG-519N NA

Cytokeratin 4 EP4 Esophagus FG-717N FB-717N

Cytokeratin 4 6B10 Tonsil FG-705M FB-705M

Cytokeratin 5 EPR1600Y Squamous Cell Carcinoma NA NA

Cytokeratin 5 & 6 EPR1600Y & EPR1602Y Squamous Cell Carcinoma FG-588N NA

Cytokeratin 5 & Cytokeratin 14 EP24/EP61 Prostate FG-730N FB-730N

Cytokeratin 6 EPR1602Y Squamous Cell Carcinoma FG-500N NA

Cytokeratin 7 OV-TL12/30 Adenocarcinoma FG-255M FB-255M

Cytokeratin 7 EPR1619Y Breast Cancer FG-528N NA

Cytokeratin 7 & 8 OV-TL12/30 & C51 Breast Carcinoma FG-587M NA

Cytokeratin 8 C-51 Breast Carcinoma FG-142M NA

Cytokeratin 8 & 18 5D3 Colon Carcinoma FG-131M FB-131M

Cytokeratin 10 DEK-10 Skin FG-201M NA

Cytokeratin 13 AE8 Tongue/Esophagus/Tonsil FG-132M NA

Cytokeratin 14 LL002 Squamous Cell Carcinoma FG-146M NA

Cytokeratin 15 EPR1614Y Squamous Cell Carcinoma NA NA

Cytokeratin 16 EP1615Y Squamous Cell Carcinoma NA NA

Cytokeratin 17 E27Basal Cell Carcinoma and Squa-mous Cell Carcinoma Tissue

FG-572M NA

Cytokeratin 18 DC-10 Breast Carcinoma FG-143M NA

Cytokeratin 19 RCK108 Colon Carcinoma FG-246M NA

Cytokeratin 20 IT-Ks20.8 Colon Carcinoma FG-315M NA

Cytokeratin 20 EPR1622Y Colon Carcinoma FG-557N NA

Cytokeratin Cocktail AE1 and AE3 Skin FG-071M FB-071M

Cytokeratin cocktail, Broad Spectrum 34ßE12/C51/AE1 Skin FG-273M NA

Cytokeratin cocktail, Broad SpectrumLL002+DEK-10+RCK108+OV-TL12/30+C11

Breast Carcinoma FG-372M FB-372M

Cytokeratin, High MW 34ßE12 Prostate FG-291M FB-291M

Cytokeratin, High MW (Basic) AE3 Squamous Cell Carcinoma FG-133M NA

Cytokeratin, Low MW AE1 Adenocarcinoma FG-075M FB-075M

Cytokeratin, Pan Lu-5 Colon Carcinoma FG-181M NA

Cytokeratin, Pan C11 Breast Carcinoma NA NA

Cytomegalovirus (CMV) BM204 CMV Infected Cells/Lungs NA NA

Desmin D33 Leiomyoma FG-072M FB-072M

DOG1 1.1 GIST Tissues FG-570M NA

Dystrophin Dys1(Dy4/6D3) Muscle FG-243M NA

Dystrophin Dys2(Dy8/6C5) Muscle FG-244M NA

E-Cadherin 36Breast Carcinoma/Colon Carci- noma

NA NA

E-Cadherin EP6 Colon FG-725N FB-725N

188

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EGFR Polyclonal Squamous Cell Carcinoma NA NA

EGFR(PAN) EP38Y Squamous Cell Carcinoma NA NA

Ep-CAM E144 Adenoma FG-489N NA

Epithelial Mem Antigen (EMA) E29 Lung FG-057M FB-057M

Epithelial Mem Antigen (EMA) Mc5 Breast Cancer FG-182M NA

Epithelial-Specific Antigen MOC-31 Colon Carcinoma FG-316M NA

Epstein-Barr Virus Early Antigen 1108-1 EBV Infected Culture Cells FG-222M NA

ErbB2/HG2 EP1045Y(R) NA NA NA

Estradiol Polyclonal Breast Carcinoma FG-038P NA

Estrogen Receptor (ER Alpha) EPR703(2) Breast Carcinoma FG-509M NA

Estrogen Receptor 1D5 Breast Carcinoma FG-272M FB-272M

Estrogen Receptor EP1(R) NA FG-710N FB-710N

Estrogen Receptor (InSite® ER) ER88 Breast Carcinoma FG-368M FB-368M

Estrogen Receptor-Beta (ER-β) Polyclonal Breast Carcinoma NA NA

Factor VIII-Related antigen F8 2.2.9 Leiomyoma FG-016M FB-016M

Factor XIII Subunit A E980.1 Placenta FG-337M NA

Factor XIII A EP3372 Bone Marrow FG-516N NA

Fascin FCN01 Lymph Node FG-488M NA

Fibronectin F14 Placental Tissue FG-569N NA

Follicle Stimulating Hormone (FSH) Polyclonal Pituitary NA NA

Gastrin Polyclonal Gastrin FG-019P NA

GCDFP-15 EP1582Y Breast Carcinoma FG-481N NA

Glial Fibrillary Acidic Protein (GFAP) GA-5 Cerebellum FG-020M FB-020M

Glial Fibrillary Acidic Protein (GFAP) Polyclonal Cerebellum FG-020P NA

Glomerular Epithelial Protein 1 (GLEPP-1) 5C11 Kidney NA NA

Glucagon Polyclonal Pancreas FG-039P NA

Glut-1 SPM498Esophagus Carcinoma/ Squamous Carcinoma

FG-505M NA

Glutathione S-Transferase pi (GST Pi) Polyclonal Breast FG-249P NA

Glypican-3 (GPC3) GPC3-88 Hepatocellular Carcinoma FG-539M NA

Granulocyte BM-2 Hodgkin FG-210M NA

Growth Hormone (Hgh) Polyclonal Pituitary NA NA

Heat Shock Protein (HSP-70) BRM-22 Breast Carcinoma FG-289M NA

Heat Shock Protein 27 (HSP 27) G3.1 Breast Carcinoma FG-171M FB-171M

Helicobacter Pylori Polyclonal H. Pylori Infected Stomach Tissue FG-442P NA

Hemoglobin A Polyclonal Placenta FG-021P NA

Hepatitis B Virus Core Antigen (HBcAg) Polyclonal Infected Liver FG-082P NA

Herpes Simplex Virus Type I (HSV I) Polyclonal Infected Lung NA NA

Herpes Simplex Virus Type II (HSV II) Polyclonal Infected Lung NA NA

HGH Polyclonal Placenta/Pituitary Gland FG-707P FB-707P

HLA-DR LN3 Tonsil FG-154M NA

HSA HSA/E8 Liver Tissues FG-550M NA

Human Chorionic Gonadotropin (hCG) Beta M94138 Placenta NA NA

IgA Polyclonal Tonsil NA NA

IgD Polyclonal Tonsil FG-440P NA

IgG IgG88 Tonsil FG-367M NA

IgG Polyclonal Tonsil FG-050P NA

IgM IgM88 Tonsil FG-366M NA

IgM Polyclonal Tonsil FG-427P FB-427P

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Inhibin-Alpha R1 Ovary FG-446M NA

Insulin HB125 Pancreas FG-029M NA

Insulin EP125 Pancreas/Insulinoma FG-735N FB-735N

J Chain JC88 Tonsil NA NA

Kappa Light Chain L1C1 Tonsil FG-048M NA

Kappa Light Chain K88 Tonsil FG-369M FB-369M

Ki-67 K-2 Tonsil FG-410M FB-410M

Ki-67 EPR3611 Breast Carcinoma/Tonsil FG-527N NA

Ki-67 EP5 Tonsil FG-727N FB-727N

Ki-67 and Lambda light chain cocktail K-2 and Polyclonal Tonsil Tissues FG-562C NA

Ki-67 Antigen,Proliferating Cell MIB-1 Lymphoma FG-297M NA

Ki-67 Antigen,Proliferating Cell Ki88 Tonsil FG-370M FB-370M

Lambda Light Chain Polyclonal Tonsil FG-049P NA

Lambda Light Chain EP172 Tonsil/B-cell Lymphoma FG-715N FB-715N

Laminin Polyclonal Bronchus FG-078P FB-078P

Luteinizing Hormone (LH) Polyclonal Pituitary NA NA

Lysozyme Polyclonal Tonsil/Lymph Node FG-024P FB-024P

Macrophage LN5 Liver Tissues FG-165M NA

Mast Cell Tryptase AA1 Skin FG-419M FB-419M

Melan-A (MART-1) A103 Melanoma FG-361M FB-361M

Melanoma HMB45 Melanoma FG-001M FB-001M

Melanoma gp100 gp100/D5 Melanoma Tissue FG-536M NA

Melanoma-Associated Antigen NKI/C3 Melanoma FG-077M FB-077M

Mesothelin 5B2 Ovary Cyst Adenocarcinoma FG-433M NA

MiTF MiTF/A13 Melanoma Tissue FG-554M NA

Mitochondrial Antigen 113-1 Breast Carcinoma NA NA

MLH1 ES05 Colon Tissue FG-703M FB-703

MMP-9 EP1255Y Bone Marrow FG-504N NA

MSH2 SP46 Colon NA NA

MSH6 2D4B5 Colon Carcinoma FG-454M NA

MUC4 1G8 Colon Carcinoma FG-455M NA

MUC5AC 45M1Stomach Mucosa/Gastrointestinal Tissue

FG-456M NA

Mucin 1 (MUC1) EPR1023 Breast Carcinoma FG-564N NA

Mucin 2 (MUC2) CCP58 Colon Carcinoma NA NA

Muscle Actins Actin 88 Cocktail Muscle NA NA

Myelin Basic Protein MBP88 Cerebellum NA NA

Myeloid specific Antigen BM-3 Bone Marrow NA NA

Myeloid specific Antigen BM-1 Lymph node/Tonsil/Bone Marrow FG-164M NA

Myeloperoxidase (MPO) Polyclonal Spleen FG-496P NA

Myf4 LO26 Rhabdomyosarcoma FG-432M NA

Myoglobin MG-1 Muscle FG-012M NA

Myoglobin Polyclonal Muscle FG-012P NA

Myosin Heavy Chains,Smooth Muscle SMMS.1 Breast Carcinoma NA NA

Myosin,Skeletal Muscle MY-32 Muscle FG-109M NA

Napsin A IP64 Lung Adenocarcinoma FG-701M FB-701M

N-Cadherin EPR1792Y Colon Carcinoma Tissue FG-565N NA

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Neurofilament NE-14 Nerve FG-073M FB-073M

Neuron Specific Enolase (NSE) MIG-N3 Pancreas/Nerve FG-055M FB-055M

NGF Receptor EP1039Y(R) Neuroblastoma NA NA

NGFR EP31 Pancreas/Melanoma FG-738N FB-738N

Nuclear Ribonucleoprotein 58-15 Spleen FG-230M NA

Oct 2 EPR542 Tonsil tissues FG-558N NA

Oct 4 EP143 Seminoma in Testis FG-724N FB-724N

Osteonectin OST1 Osteosarcoma NA NA

p105 Proliferation-Associated Nuclear Antigen 2B3 Tonsil NA NA

p16 (INK4a) G175-405 Sqamous Cell Carcinoma FG-540M NA

p16+Ki67 cocktail G175-405 +EPR3611 Cervical Carcinoma FG-601C FB-601C

p21/WAF1 4D10 Melanoma FG-434M NA

p27 (Kip1) DCS72 Breast Carcinoma NA NA

p27 (Kip1) Y236 Breast Carcinoma FG-492N NA

p34cdc2 (Cyclin Dependent Kinase) POH-1 Tonsil FG-301M NA

P504S (AMACR) RBT-AMACR Prostate Carcinoma FG-538N NA

P504S (AMACR) 13H4 Prostate Carcinoma FG-449N NA

p53 EP9 Breast Ca. FG-728N FB-728N

p53 Protein BP53-12-1 Breast Carcinoma FG-195M FB-195M

p53 Protein DO7 Breast Carcinoma FG-239M FB-239M

p53 Protein 1801 Breast Carcinoma FG-240M NA

p63 Protein 4A4(M) Prostate Carcinoma FG-418M FB-418M

PAP A40010Benign Prostate Hyperplasia(BPH) and Carcinoma Prostate

FG-532M NA

Papillomavirus Type 16 (HPV-16) CamVir-1 HPV-16 Infected Cells/Tissue NA NA

PAX-5 ZP007 Lymph Node FG-457M NA

Paxillin (N-Term) Y113 Breast Carcinoma FG-521N NA

PDCD4 EPR3432 Breast Carcinoma FG-524N NA

PDGF Receptor-ß Y92 GIST FG-463N NA

PGP9.5 3D9 Brain/Small Intestine FG-736M FB-736M

PIN4 4A4+34ßE12+13H4 Prostate Carcinoma FG-448M NA

Placental Alkaline Phosphatase (PLAP) PL8-F6 Placenta FG-228M FB-228M

Placental Lactogen (hPL) Polyclonal Placenta FG-040P NA

Platelet-Derived Growth Factor (PDGF) PDGF88 Squamous NA NA

Platelet-Derived Growth Factor (PDGF) Polyclonal Squamous NA NA

PMS2 EPR3947 Colon Carcinoma FG-577N NA

Progesterone Receptor EP2(R) NA FG-711N FB-711N

Progesterone Receptor 1A6 Breast Carcinoma FG-172M NA

Progesterone Receptor (InSite®PR) PR88 Breast Carcinoma FG-328M FB-328M

Prolactin ME.121 Pituitary NA NA

Proliferating Cell Nuclear Antigen (PCNA) PC10 Colon Carcinoma FG-252M FB-252M

Prostate Specific Acid Phosphatase (PSAP) B01-94-21M-NA Prostate Hyperplasia FG-013M FB-013M

Prostate Specific Antigen (PSA) ErPr-8 Prostate Carcinoma NA FB-014M

pS2 Estrogen Inducible Protein PS2.1 Breast Carcinoma FG-190M FB-190M

PSMA EP192 Prostate Ca. FG-714N FB-714N

RCC (Renal Cell Carcinoma) RCC-26 Kidney,Renal Cell Carcinoma FG-543M NA

S100 beta EP32 Prostate Ca. FG-713N FB-713N

S-100 Protein 15E2E2 Melanoma FG-058M FB-058M

S-100 Protein Polyclonal Melanoma FG-058P NA

191

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S100P EP186 Placenta/Pancreatic Adeno Ca. FG-712N FB-712N

Sarcomeric Actin ZMSA-5 Muscle FG-511M NA

Secretin Polyclonal Stomach FG-067P NA

SOX2 EPR3131 Sqamous Cell Carcinoma FG-579N NA

Stat 5-Alpha (C-term) E289 Breast Carcinoma FG-480N NA

Substance P Polyclonal Hypothalamus FG-069P NA

Survivin EP2880YTCC Bladder Tissue/Bladder Carcinoma

FG-503N NA

Synaptophysin Snp88 Pancreas FG-363M FB-363M

Tau Tau-2 Cerebellum FG-412M FB-412M

Tau Tau-5 Brain FG-459M FB-459M

Terminal Deoxynucleotidyl Transferase (TdT) TdT88 Thymus NA NA

Terminal Deoxynucleotidyl Transferase (TdT) Polyclonal Thymus FG-373P NA

TFPU.1 EPR3159Y Lymphoma FG-520N NA

Thyroglobulin 2H11 Follicular Adenoma FG-032M NA

Thyroid Stimulating Hormone (TSH) 5404 Follicular Adenoma NA NA

Thyroid Stimulating Hormone (TSH) Polyclonal Follicular Adenoma NA NA

Thyroid Transcription Factor (TTF-1) 8G7G3/1 Lung FG-397M NA

Thyroxine D5 Thyroid FG-034M NA

Topo 2 Alpha EP1102YThyroid Carcinoma/Breast Carci- noma)

FG-576N NA

Toxoplasma gondii Polyclonal Infected Cells/Tissues FG-125P NA

Transferrin HT1/13.6.3 Liver/Muscle FG-025M NA

Transforming Growth Factor (TGF),Alpha TGF88 Breast Carcinoma NA NA

TTF-1 and Cytokeratin 5/6 cocktail8G7G3/1, EPR1600Y,EPR1602Y

Lung/Squamous FG-563C NA

TTF-1+CK20+CK7 cocktail8G7G3/1+EPR1622Y +EPR1619Y

Lung Carcinoma NA NA

TTF-1+GCDFP-15 cocktail 8G7G3/1+EP1582Y Lung Carcinoma FG-604C NA

Tumor-Associated Glycoprotein (TAG-72) B72.3 Breast Carcinoma FG-054M FB-054M

Tumor-Associated Glycoprotein (TAG-90 BCA) B6.2 Breast Carcinoma FG-005M NA

Tyrosinase Ty/G5 Melanoma FG-535M NA

VEGF Polyclonal Angiosarcoma FG-483P NA

Villin ID2C3 Small Intestine FG-525M NA

Vimentin V9 Skin/Leiomyoma FG-074M FB-074M

Vimentin SP20 Leiomyoma FG-566N NA

Vimentin,Non-Hematopoietic LN6Submucosal Vascular Endothelial cells/Leiomyoma

FG-163M NA

VIP Polyclonal Small Intestine FG-530P NA

Wilm’s Tumor CAN-R9(IHC)-56-2 Wilm’s Tumor FG-584N NA

ZAP-70 ZAP70-C3 Tonsil and Reactive Lymph Node FG-544M NA

ZAP-70 YE291 Tonsil FG-466N NA

NOTE: The list for positive control slides is constantly being updated, depending upon tissue availability. Please call 1(800) 421-4149 for availability or visit our website at www.biogenex.com

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194

Alphabetical Product Name Index

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Description Clone Cat. No Pack Size Page

AAberrant Endothelial Cell 4A11 AM382-5M, AX382-YCD, MU382-UC 6ml, 16 ml, 1ml 11, 43, 51

Acid Fast Blue Stain Kit - SS025-50X 50 Slides 168, 173

ACTH AH26 AM487-5M, AM487-10M, AX487-YCD, MU487-UC 6 ml, 10 ml, 16 ml, 1 ml

11, 23, 43, 51, 184

Actin, Muscle-Specific HHF35 AM090-5M, AM090-10M, AX090-YCD, MU090-UC 6ml, 10ml, 16 ml, 1ml 11, 23, 43, 51, 184

Actin, Smooth Muscle 1A4 AM128-5M, AM128-10M, AX128-YCD, MU128-UC 6ml, 10ml, 16 ml, 1ml 11, 23, 43, 51, 184

Adenovirus A62020069P AM059-5ME, AX059-YCDE, MU059-UCE 6ml, 10 ml, 1ml 11, 43, 52

AEC Chromogen - HK139-06K 6ml 178

AEC Chromogen - HK139-50KE 50ML 178

AEC Substrate Kit, Conc - HK129-5KE 2500 Slides 178

AEC Substrate Pack Kit - HK092-5K 250 Slides 178

AFB Green - SS059-50X 50 Slides 168, 173

Alcian Blue PAS Stain - SS026-50X, SS026-YAK 50 Slides, 50 Slides 168, 173, 174

Alcian Blue Stain - SS027-50X, SS027-YAK 50 Slides, 50 Slides 168, 173, 174

Alizarin Red Stain - SS052-50K 50 Slides 168, 174

Alpha-1-Antichymotrypsin a1A88 AM388-5M, AX388-YCD, MU388-UC 6ml, 16 ml, 1ml 11, 43, 52

Alpha-1-Antitrypsin Polyclonal AR015-5R, AW015-YCD, PU015-UP 6ml, 16 ml, 1ml 11, 43, 52, 184

Alpha-Actinin JLN20 AM097-5M, AX097-YCD, MU097-UC 6ml, 16 ml, 1ml 11, 43, 52, 184

Alpha-Fetoprotein (AFP) C3 AM008-5M, AM008-10M, AX008-YCD, MU008A-UC 6ml, 10ml, 16 ml, 1ml 11, 23, 43, 53, 184

Alpha-Tubulin DM-1A MU121-UC 1ml 43, 53, 184

ALU Probe II - PR026-100E, PR026-YADE 0.650 ml, 0.650 ml 19, 150, 151

Androgen Receptor F39.4.1 AM256-2ME, AM256-10ME, AX256-YCDE, MU256-UCE 3ml, 10ml, 16ml,1ml 11, 23, 43, 53,

184

Antigen Retrieval AR-10 (10X) - HK057-5KE 100 ml 37

Antigen Retrieval AR-10 RTU - HK058-5K, HK058-20K 250 ml, 1L 37

Antigen Retrieval Citra (10X) - HK086-5K, HK086-9K 100 ml, 500 ml 36

Antigen Retrieval Citra Plus (10X) - HK080-5K, HK080-9K 100 ml, 500 ml 36

Antigen Retrieval Citra Plus RTU - HK081-5KE, HK081-20KE 250 ml, 1L 36

Antigen Retrieval Citra RTU - HK087-5KE, HK087-20KE 250 ml, 1L 36

Apolipoprotein E EP1373Y AN567-5M, AN567-10M, AX567-YCD, NU567-UC 6 ml, 10 ml, 16 ml, 1 ml

11, 23, 43, 53, 184

Aqueous Mounting Medium - HK099-5K 15ml 180

Auto DAB kit for Xmatrx Elite - XT150-YCDE 200 slides 18

AutoFISH Pretreatment Reagent - HK983-YRXE 500 ml 163

AutoFISH Protease 1 Buffer - HK981-YRXE 500 ml 163

AutoFISH Protease 2 Buffer - HK982-YRXE 500 ml 163

AutoFISH Reagent A - HK972-YRXE 500 ml 163

AutoFISH Reagent B - HK973-YRXE 500 ml 163

Avidin Biotin Blocking Kit - HK102-5K, HK102-10K, HK102-20X 60 Slides, 100 Slides, 200 Slides 28, 177

Azure A satin - SS038-50X, SS038-YAK 50 Slides, 50 Slides 169, 173, 174

BB Cell MB2 AM158-5M, AM158-10M, AX158-YCD, MU158-UC 6ml, 10ml, 16 ml, 1ml 11, 23, 43, 54,

184

B Lymphocyte Antigen 36 (BLA.36) A27-42 AM231-5M, AM231-10M, AX231-YCD, MU231-UC 6ml, 10ml, 16 ml, 1ml 11, 23, 43, 54, 184

Barrier Slides (25x25mm) - XT108-CL, XT108-SL 1440 Slides, 72 slides 20, 22

Barrier Slides, 18X18mm, for Xmatrx - XT128-CL, XT128-SL 1440 Slides, 72 slides 20, 22

Barrier Slides, 2 Zones, for Xmatrx - XT114-CL, XT114-SL 1440 Slides, 72 slides 20, 22

Barrier Slides, 25X40mm, for Xmatrx - XT134-CL, XT134-SL 1440 Slides, 72 slides 20, 22, 29, 181

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Bax Protein Polyclonal AR347-5R, AW347-YCD, PU347-UP 6ml, 16 ml, 1ml 11, 43, 54

Bcl-2 E17 AN541-5M, AN541-10M, AX541-YCD, NU541-UC 6 ml, 10 ml, 16 ml, 1 ml

11, 23,43, 54, 184

Bcl-2 EP36 AN723-5M, AN723-10M, AY723-YCD, NU723-UC 6 ml, 10 ml, 16ml, 1ml 11, 23, 43, 55, 184

Bcl-2 Oncoprotein bcl-2/100 AM287-5M, AM287-10M, AX287-YCD 6ml, 10ml, 16 ml 11, 23, 43, 55, 184

Bcl-6 LN22 AM708-5M, AM708-10M, AY708-YCD, MU708-UC 6 ml, 10 ml, 16ml, 1ml 11, 23, 43, 55

Bcl-x E18 AN462-5M, AN462-10M, AX462-YCD, NU462-UC 6 ml, 10 ml, 16 ml, 1ml 11, 23, 43, 55, 184

Beta Actin - PR1055-100E, PR1055-YADE 0.650 ml, 0.650 ml 19, 150, 151

Beta-Catenin E247 AN510-5M, AN510-10M, AX510-YCD, NU510-UC 6 ml, 10 ml, 16 ml, 1 ml

11, 23, 43, 56 ,184

Beta-Human Chorionic Gonadotropin (hCG) M94138 AM395-5M, AX395-YCD, MU395-UC 6ml, 16 ml, 1ml 15, 48, 103

Beta-Tubulin DM-1B MU122-UC 1ml 43, 56, 184

Beta-Tubulin II JDR3B8 MU176-UC 1ml 43, 56, 184

Beta-Tubulin III SDL3D10 MU177-UC 1ml 43, 56, 184

Beta-Tubulin IV ONS1A6 MU178-UC 1ml 43, 57, 184

bFGF (Basic Fibroblast Growth Factor) bFGF88 AM359-5M, AX359-YCD 6ml, 16 ml 11, 43, 57

Bielschowsky's Silver Stain - SS055-50X, SS055-YAK 50 Slides, 50 Slides 169, 173, 174

Blocking Reagent II Goat Serum - HK112-5K, HK112-9KE 6ml, 50 ml 177

Blocking Reagent II Rabbit Serum - HK114-5K 6ml 177

Blood Group Antigen Lewis A 7LE MU303-UC 1ml 43, 57, 184

Blood Group Antigen Lewis B 2-25LE MU304-UC 1ml 43, 57, 184

BOB1 SP92 AN731-5ME, AN731-10ME, AY731-YCDE, NU731-UCE 6ml, 10ml, 16ml, 1ml 11, 23, 43, 58

BRCA1 Protein Polyclonal AR345-5R, AW345-YCD, PU345-UP 6ml, 16 ml, 1ml 11, 43, 55

Breast Cancer Antigen BCA 225 CU18 AM135-5M, AM135-10M, AX135-YCD 6ml, 10ml, 16 ml 11, 23, 43, 58, 184

CCA125 Ov185:1 AM429-5M, AM429-10M, AX429-YCD, MU429-UC 6ml, 10ml, 16 ml, 1ml 11, 23, 43, 59,

184

CA19-9 C241:5:1:4 AM424-5M, AM424-10M, AX424-YCD, MU424-UC 6ml, 10ml, 16 ml, 1ml 11, 23, 43, 58, 184

Caldesmon E89 AN507-5M, AN507-10M, AX507-YCD, NU507-UC 6 ml, 10 ml, 16 ml, 1 ml

11, 23, 43, 59, 184

Caldesmon, High MW, Smooth muscle h-CD AM332-5M, AX332-YCD, MU332-UC 6ml, 16 ml, 1ml 11, 43, 59

Calponin CALP AM333-5M, AX333-YCD, MU333-UC 6ml, 16 ml, 1ml 11, 43, 59, 184

Calponin-1 (C-term) EP798Y AN502-5M, AN502-10M, AX502-YCD, NU502-UC 6 ml, 10 ml, 16 ml, 1 ml

11, 23, 43, 60, 184

Calretinin 2E7 AM583-5M, AM583-10M, AX583-YCD, MU583-UC 6 ml, 10 ml, 16 ml, 1 ml

11, 23, 43, 60, 184

Calretinin Polyclonal AR413-2R, AR413-5R,AR413-10R, AW413-YCD, PU413-UP

3ml, 6ml, 1ml, 10ml, 16 ml, 1ml 11, 23, 60, 184

Cap for Optimiser Vials (24) - XT022-CP 24/pk 28

Catenin (p120) YE372 AN523-5M, AN523-10M, AX523-YCD, NU523-UC 6 ml, 10 ml, 16 ml, 1 ml

16, 23, 44, 61, 185

Catenin Delta 1 (p120) Polyclonal AR706-5R, AR706-10R, AW706-YCD, PU706-UP 6ml, 10 ml, 16 ml, 1ml 11, 23, 44, 61, 185

Cathepsin D C15 AM467-5M, AX467-YCD, MU467-UC 6 ml, 16 ml, 1 ml 11, 44, 62

CD1a O10 AM490-5M, AM490-10M, AX490-YCD, MU490-UC 6 ml, 10 ml, 16 ml, 1 ml

11, 23, 44, 62, 185

CD2 AB75 AM438-5M,AX438-YCD, MU438-UC 6ml,16ml, 1 ml 11, 44, 62, 185

CD3 (T Cell) UCHT1 AM258-5M, AX258-YCD, MU258-UC 6ml, 16 ml, 1ml 12, 44, 63

CD3 (T Cell) PS1 AM322-5M, AM322-10M, AX322-YCD, MU322-UC 6ml, 10ml, 16 ml, 1ml 12, 23, 44, 63, 185

CD3 and CD20 cocktail Mouse L26 + Rabbit EP449E AC559-5M, AC559-10M, AC559-YCD 6 ml, 10 ml, 16 ml 12, 23, 145,

185

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CD3 Epsilon EP449E AN477-5M, AN477-10M, AX477-YCD, NU477-UC 6 ml, 10 ml, 16 ml, 1 ml

12, 23, 44, 62, 185

CD4 4B12 AM421-5M, AM421-10M, AX421-YCD, MU421-UC 6ml, 10ml, 16 ml, 1ml 12, 23, 44, 63, 185

CD4 EP204 AN722-5M, AN722-10M, AY722-YCD, NU722-UC 6 ml, 10 ml, 16mL, 1mL

12, 23, 44, 63, 185

CD4 + C8 Cocktail Mouse 4B12 + Rabbit EP1150Y AC595-5M, AC595-10M, AC595-YCD 6 ml, 10 ml, 16 ml 12, 23, 145

CD5 4C7 AM430-5M, AM430-10M, AX430-YCD, MU430-UC 6ml, 10ml, 16 ml, 1ml 12, 23, 44, 64, 185

CD5 EP2952 AN512-5M, AN512-10M, AX512-YCD, NU512-UC 6 ml, 10 ml, 16 ml, 1 ml

12, 23, 44, 64, 185

CD7 LP15 AM702-5M, AM702-10M, AY702-YCD, MU702-UC 6ml, 10ml, 16 ml, 1ml 12, 23, 44, 64, 185

CD8 (T Cell,Suppressor/Cytotoxic) T8 AM261-5M, AX261-YCD, MU261-UC 6ml, 16 ml, 1ml 12, 44, 65

CD8 1A5 AM422-5M, AM422-10M, AX422-YCD, MU422-UC 6ml, 10ml, 16 ml, 1ml 12, 23, 44, 64, 185

CD8 SP16 AN740-5M, AN740-10M, AY740-YCD, NU740-UC 6ml , 10ml, 16ml, 1ml 12, 23, 44, 65

CD8 (C-Term) EP1150Y AN513-5M, AN513-10M, AX513-YCD, NU513-UC 6 ml, 10 ml, 16 ml, 1 ml

12, 23, 44, 65, 185

CD10 56C6 AM451-5M, AM451-10M, AX451-YCD, MU451-UC 6 ml, 10 ml, 16 ml, 1 ml

12, 23, 44, 65, 185

CD10 and Cyclin D1 cocktailMouse 56C6 + Rabbit EPR2241 (IHC)-32

AC561-5M, AC561-10M, AC561-YCD 6 ml, 10 ml, 16 ml 12, 24, 145, 185

CD11b (C3bi Receptor) M01 AM270-5M, AX270-YCD 6ml, 16 ml 12, 44, 66

CD11b/ITAM EPR1344 AN546-5M, AN546-10M, AX546-YCD, NU546-UC 6 ml, 10 ml, 16 ml, 1 ml

12, 24, 44, 66, 185

CD11c EP1347Y AN555-5M, AN555-10M, AX555-YCD, NU555-UC 6 ml, 10 ml, 16 ml, 1 ml

12, 24, 44, 66, 185

CD14 EPR3653 AN515-5M, AN515-10M, AX515-YCD, NU515-UC 6 ml, 10 ml, 16 ml, 1 ml

12, 24, 44, 65, 185

CD15 BRA4F1 AM302-5M, AM302-10M, AX302-YCD, MU302-UC 6ml, 10ml, 16 ml, 1ml 12, 24, 44, 67, 185

CD16 2H7 AM437-5M, AX437-YCD, MU437-UC 6ml, 16 ml, 1ml 12, 44, 67, 185

CD19 EP169 AN729-5M, AN729-10M, AY729-YCD, NU729-UC 6 ml, 10 ml, 16ml, 1ml 12, 24, 44, 67, 185

CD20 CD20/C23 AM537-5M, AM537-10M, AX537-YCD, MU537-UC 6 ml, 10 ml, 16 ml, 1 ml

12, 24, 44, 67, 185

CD20 (B Cell) L-26 AM238-5M, AM238-10M, AX238-YCD, MU238A-UC 6ml, 10ml, 16 ml, 1ml 12, 24, 44, 185

CD20 (B Cell) B1 AM265-5M, AX265-YCD, MU265-UC 6ml, 16 ml, 1ml 12, 44, 68

CD21 EP3093 AN497-5M, AN497-10M, AX497-YCD, NU497-UC 6 ml, 10 ml, 16 ml, 1 ml

12, 24, 44, 68, 185

CD21 (B Cell) B2 AM266-5M, AX266-YCD 6ml, 16 ml 12, 44, 68

CD22 FPC1 AM439-5M, AX439-YCD, MU439-UC 6ml, 16 ml, 1ml 12, 44, 69, 185

CD23 Polyclonal AR460-5R, AR460-10R, AW460-YCD, PU460-UP 6 ml, 10 ml, 16 ml, 1 ml

12, 24, 44, 69, 185

CD25 4C9 AM453-5M, AM453-10M, AX453-YCD, MU453-UC 6 ml, 10 ml, 16 ml, 1 ml

12, 24, 44, 69, 185

CD29 JB1a AM298-5M, AX298-YCD, MU298-UC 6ml, 16 ml, 1ml 12, 44, 69, 185

CD30 (Ki-1 Antigen) HRS-4 AM351-5M, AM351-10M, AX351-YCD, MU351-UC 6ml, 10ml, 16 ml, 1ml 12, 24, 44, 70

CD30 (Ki-1 Antigen) Ber-H2 AM327-5M, AM327-10M, AX327-YCD 6ml, 10ml, 16 ml 12, 24, 44, 70, 185

CD31 JC/70A AM232-5M, AM232-10M, AX232-YCD 6ml, 10ml, 16 ml 12, 24, 44, 70, 185

CD31 9G11 AM241-5M, AX241-YCD, MU241-UC 6ml, 16 ml, 1ml 12, 44, 70, 185

CD34 (Endothelial Cell) QBend/10 AM236-5M, AM236-10M, AX236-YCD, MU236-UC 6ml, 10ml, 16 ml, 1ml 12, 24, 44, 71, 185

CD35 RLB25 AM431-5M, AM431-10M, AX431-YCD, MU431-UC 6ml, 10ml, 16 ml, 1ml 12, 24, 44, 71, 185

CD35 SP191 AN741-5M, AN741-10M, AY741-YCD, NU741-YCD 6ml , 10ml, 16ml, 1ml 12, 24, 44, 71

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CD41/Integrin Alpha IIb EP178 AN732-5ME, AN732-10ME, AY732-YCDE, NU732-UCE 6ml , 10ml, 16ml, 1ml 12, 24, 44, 71

CD43 & CD45RA Cocktail MT1 & MB1 AM159-5M, AX159-YCD 6ml, 16 ml 12, 44, 72, 186

CD43 (T Cell, Leukosialin) DFT-1 AM305-5M, AX305-YCD, MU305-UC 6ml, 16 ml, 1ml 12, 44, 72, 186

CD44 (Phagocytic Glycoprotein-1,HCAM) DF1485 AM310-5M, AX310-YCD, MU310-UC 6ml, 16 ml, 1ml 12, 45, 72, 186

CD45 PD7/26/16 & 2B11 AM111-5M, AM111-10M, AX111-YCD 6ml, 10ml, 16 ml 12, 24, 45, 72,

186

CD45 (Leukocyte common Antigen,LCA) LJ27.9 AM338-5M, AX338-YCD, MU338-UC 6ml, 16 ml, 1ml 12, 45, 73

CD45 Cocktail (Leukocyte Antigen, LCA) MEM55+LJ27.9 AM371-5M, AM371-10M, AX371-YCD, MU371-UC 6ml, 10ml, 16 ml, 1ml 12, 24, 45, 73, 186

CD45RA (B Cell) MB1 AM157-5M, AM157-10M, AX157-YCD 6ml, 10ml, 16 ml 12, 24, 45, 73, 186

CD45RB MEM55 AM320-5M, AX320-YCD, MU320-UC 6ml, 16 ml, 1ml 12, 45, 73, 186

CD45RC (T Cell) MT2 AM156-5M, AX156-YCD, MU156-UC 6ml, 16 ml, 1ml 12, 45, 74, 186

CD45RO (T Cell) UCHL-1 AM113-5M, AM113-10M, AX113-YCD, MU113-UC 6ml, 10ml, 1ml, 16 ml 12, 24, 45, 74, 186

CD48 EP148 AN721-5ME, AN721-10ME, AY721-YCDE, NU721-UCE 6ml , 10ml, 16ml, 1ml 13, 24, 45, 74

CD53 EP179 AN734-5M, AN734-10M, AY734-YCD, NU734-UC 6 ml, 10 ml, 16mL, 1mL 13, 24, 45, 74,

CD56 (Natural Killer Cell,NCAM) NKH-1 AM268-5M, AX268-YCD, MU268-UC 6ml, 16 ml, 1ml 13, 45, 75

CD57 (Natural Killer Cell) NK-1 AM314-5M, AX314-YCD, MU314-UC 6ml, 16 ml, 1ml 13, 45, 75, 186

CD61 EP2417Y AN482-5M, AN482-10M, AX482-YCD, NU482-UC 6 ml, 10 ml, 16 ml, 1 ml

13, 24, 45, 75, 186

CD63 EP211 AN720-5ME, AN720-10ME, AY720-YCDE, NU720-UCE 6ml , 10ml, 16ml, 1ml 13, 24, 45, 75,

186

CD66 BY114 AM325-5M, AX325-YCD 6ml, 16 ml 13, 45, 76, 186

CD68 CD68/G2 AM549-5M, AM549-10M, AX549-YCD, MU549-UC 6 ml, 10 ml, 16 ml, 1 ml

13, 24, 45, 76, 186

CD68 KP1 AM416-5M, AM416-10M, AX416-YCD, MU416-UC 6ml, 10ml, 16 ml, 1ml 13, 24, 45, 76, 186

CD71 (Transferrin Receptor) T9 AM269-5M, AX269-YCD 6ml, 16 ml 13, 45, 76

CD71 (Transferrin Receptor) H68.4 AM354-5M, AX354-YCD, MU354-UC 6ml, 16 ml, 1ml 13, 45, 77

CD74 (B Cell) LN2 AM153-5M, AX153-YCD, MU153-UC 6ml, 16 ml, 1ml 13, 45, 77, 186

CD79a 11E 3 AM414-5M, AM414-10M, AX414-YCD, MU414-UC 6ml, 10ml, 16 ml, 1ml 13, 24, 45, 77, 186

CD79a EP82 AN719-5M, AN719-10M, AY719-YCD, NU719-UC 6 ml, 10 ml, 16mL, 1mL

13, 24, 45, 77, 186


13, 24, 45, 78, 186

CD99 (MIC2 Antigen) HO36.1.1 AM355-5M, AM355-10M, AX355-YCD, MU355-UC 6ml, 10ml, 16 ml, 1ml 13, 24, 45, 78

CD99 EPR3097Y AN518-5M, AN518-10M, AX518-YCD, NU518-UC 6 ml, 10 ml, 16 ml, 1 ml

13, 24, 45, 78, 186

CD103 EP206 AN739-5ME, AN739-10ME, AY739-YCDE, NU739-UCE 6ml, 10ml, 16mL, 1mL 13, 24, 45, 79

CD105 4G11 AM441-5M, AX441-YCD, MU441-UC 6ml, 16 ml, 1ml 13, 45, 79, 186

CD117 YR145 AN465-5M, AN465-10M, AX465-YCD, NU465-UC 6 ml, 10 ml, 16 ml, 1ml 13, 24, 45, 79, 186

CD117 T595 AM423-5M, AM423-10M, AX423-YCD, MU423-UC 6ml, 10ml, 16 ml, 1ml 13, 24, 45, 79, 186


13, 24, 45, 80, 186

CD205 EP176 AN737-5ME, AN737-10ME, AY737-YCDE, NU737-UCE 6ml, 10ml, 16mL, 1mL 13, 24, 45, 80

CD227 (MUCIN 1) VU-4H5 AM534-5M, AM534-10M, AX534-YCD, MU534-UC 6 ml, 10 ml, 16 ml, 1 ml

13, 26, 45, 80, 186

CDw75 (B Cell) LN1 AM152-5M, AX152-YCD, MU152-UC 6ml, 16 ml, 1ml 13, 45, 80, 186

CDX-2 CDX2-88 AM392-5M, AM392-10M, AX392-YCD, MU392A-UC 6ml, 10ml, 16 ml, 1ml 13, 24, 45, 81, 186

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CDX-2+CK20+CK7 cocktailMouse 7C7-D4 + Rabbit EPR1622Y+ EPR1619Y

AC597-5M, AC597-10M, AC597-YCD 6 ml, 16 ml 13, 24, 147

CDX-2+CK7 cocktail Mouse 7C7-D4 + Rabbit EPR1619Y AC596-5M, AC596-10M, AC596-YCD 6 ml, 16 ml 13, 24, 145, 186

Carcinoembryonic Antigen (CEA) B01-94-11M-P AM009-5M, AM009-10M, AX009-YCD, MU009-UC 6ml, 10ml, 16 ml, 1ml 11, 23, 43, 60, 185

Carcinoembryonic Antigen (CEA) CEA88 AM365-5M, AM365-10M, AX365-YCD, MU365-UC 6ml, 10ml, 16 ml, 1ml 13, 23, 43, 61, 185

Carcinoembryonic Antigen (CEA) Polyclonal AR009-5R, AR009-5RSG, AW009-YCD 6ml, 1ml, 16 ml 11, 43, 61, 185

c-erbB-2 (HER-2/neu) CB11 AM134-5ME, AM134-10ME, AX134-YCDE, MU134-UCE 6ml, 10ml, 1ml 13, 24, 45, 81

c-erbB-3 (HER-3) RTJ1/A2 AM319-5M, AX319-YCD, MU319-UC 6ml, 16 ml, 1ml 13, 24, 45, 81

c-erbB-2 (HER-2/neu) EP3 AN726-5ME, AN726-10ME, AY726-YCDE, NU726-UCE 6ml, 10ml, 16mL, 1mL 13, 24, 45, 81

Chromogranin A LK2H10 AM126-5M, AM126-10M, AX126-YCD, MU126-UC 6ml, 10ml, 16 ml, 1ml 13, 24, 45, 82, 186

Chromogranin A PHE-5 AM356-5M, AM356-10M, AX356-YCD, MU356-UC 6ml, 10ml, 16 ml, 1ml 13, 24, 45, 82, 187

Claudin 1 Polyclonal AR704-5R, AR704-10R, AW704-YCD, PU704-UP 6ml, 10 ml, 16 ml, 1ml 13, 24, 45, 82, 187

Claudin-5 EP224 AN718-5M, AN718-10M, AY718-YCD, NU718-UC 6 ml, 10 ml, 16mL, 1mL

13, 24, 45, 82, 187

c-myc Protein 9E10 AM318-5M, AX318-YCD, MU318-UC 6ml, 16 ml, 1ml 13. 45, 83, 187

Collagen III HWD1.1 AM167-5M, AX167-YCD, MU167-UC 6ml, 16 ml, 1ml 13, 48, 83, 187

Collagen IV COL-94 AM379-5M, AM379-10M, AX379-YCD, MU379-UC 6ml, 10ml, 16 ml, 1ml 14, 46, 83

Colloidal Iron stain - SS054-50X, SS054-YAK 50 Slides, 50 Slides 169, 173, 174

Common Ab Diluent - HK156-5KE 100 ml 176

Conc Goat Link - HK327-UG 5 ml 142

Super Sensitive Link Label IHC Detection System, HRP - LP000-ULE 1000 Slides 141

Conc Mouse Link - HK325-UM 5 ml 142

Conc Multilink - HK268-UK 5 ml 142

Conc Rabbit Link - HK326-UR 5 ml 142

Conc Strept Label AP - HK321-UK 5 ml 142

Conc Strept Label HRP - HK320-UK 5 ml 142

Congo Red Solution - SS028-50X, SS028-YAK 50 Slides, 50 Slides 168, 173, 174

Coverslips, 25X40mm, for Xmatrx - XT118-50X 50 each 20, 22

Coverslips, 25X40mm, for Xmatrx - XT118-YRK 500 each 20, 22

Coverslips, 18X18mm, for Xmatrx - XT121-YBX 175 each 20, 22

Coverslips, 25X25mm, for Xmatrx - XT122-90X 90 each 20, 22

Coverslips, 25X25mm, for Xmatrx - XT122-YQK 900 each 20, 22

Coverslips, 18X18mm, for Xmatrx - XT121-XBK 1750 each 20, 22

Cyclin D1 Polyclonal AR447-5R,AR447-10R, AW447-YCD, PU447-UP 6ml, 10 ml, 16 ml, 1ml 13, 24, 46, 83, 187

Cyclin D1 EPR2241(IHC)-32 AN474-5M, AN474-10M, AX474-YCD, NU474-UC 6 ml, 10 ml, 16 ml, 1 ml

13, 24, 46, 84, 187

Cyclin D1 Probe - PR216-100E, PR216-YADE 0.650 ml, 0.650 ml 19, 150, 151

Cyclin E2 EP454Y AN519-5M, AN519-10M, AX519-YCD, NU519-UC 6 ml, 10 ml, 16 ml, 1 ml

13, 25, 46, 84, 187

Cytokeratin 4 6B10 AM705-5M, AM705-10M, AY705-YCD, MU705-UC 6 ml, 10 ml, 16ml, 1 ml 13, 25, 46, 84, 187

Cytokeratin 4 EP4 AN717-5M, AN717-10M, AY717-YCD, NU717-UC 6 ml, 10 ml, 16mL, 1mL

13, 25, 46, 84, 187

Cytokeratin 5 EPR1600Y AN484-5M, AN484-10M, AX484-YCD, NU484-UC 6 ml, 10 ml, 16 ml, 1 ml 13, 25, 46, 85

Cytokeratin 5 & 6 EPR1600Y + EPR1602Y AN588-5M, AN588-10M, AX588-YCD, NU588-UC 6 ml, 10 ml, 16 ml,

1 ml13, 25, 46, 85, 187

199


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Cytokeratin 5 & Cytokeratin 14 EP24 + EP61 AN730-5ME, AN730-10ME, AY730-YCDE, NU730-UCE 6ml , 10ml , 16ml, 1ml 13, 25, 46, 85

Cytokeratin 6 EPR1602Y AN500-5M, AN500-10M, AX500-YCD, NU500-UC 6 ml, 10 ml, 16 ml, 1 ml

13, 25, 46, 85, 187


13, 25, 46, 86, 187

Cytokeratin 7 OV-TL12/30 AM255-5M, AM255-10M, AX255-YCD, MU255-UC 6ml, 10ml, 16 ml, 1ml 13, 25, 46, 86, 187

Cytokeratin 7&8 OV-TL12/30 & C51 AM587-5M, AM587-10M, AX587-YCD, MU587-UC 6 ml, 10 ml, 16 ml, 1 ml

14, 25, 46, 86, 187

Cytokeratin 8 C51 AM142-5M, AM142-10M, AX142-YCD, MU142-UC 6ml, 10ml, 16 ml, 1ml 14, 25, 46, 86, 187

Cytokeratin 8 & 18 5D3 AM131-5M, AM131-10M, AX131-YCD, MU131-UC 6ml, 10ml, 16 ml, 1ml 14, 25, 46, 87, 187

Cytokeratin 10 DEK-10 AM201-5M, AX201-YCD, MU201-UC 6ml, 16 ml, 1ml 14, 46, 87, 187

Cytokeratin 13 AE8 AM132-5M, AX132-YCD 6ml, 16 ml 14, 46, 87, 187

Cytokeratin 14 LL002 AM146-5M, AM146-10M, AX146-YCD, MU146-UC 6ml, 10ml, 16 ml, 1ml 14, 25, 46, 87, 187

Cytokeratin 15 EPR1614Y AN485-5M, AN485-10M, AX485-YCD, NU485-UC 6 ml, 10 ml, 16 ml, 1 ml 14, 25, 46, 88

Cytokeratin 16 EP1615Y AN486-5M, AN486-10M, AX486-YCD, NU486-UC 6 ml, 10 ml, 16 ml, 1 ml 14, 25, 46, 88

Cytokeratin 17 E27 AM572-5M, AM572-10M, AX572-YCD, MU572-UC 6 ml, 10 ml, 16 ml, 1 ml

14, 25, 46, 88, 187

Cytokeratin 18 DC-10 AM143-5M, AX143-YCD, MU143-UC 6ml, 16 ml, 1ml 14, 46, 88, 187

Cytokeratin 19 RCK108 AM246-5M, AM246-10M, AX246-YCD, MU246-UC 6ml, 10ml, 16 ml, 1ml 14, 25, 46, 89, 187


14, 25, 46, 89, 187

Cytokeratin 20 IT-Ks20.8 AM315-5M, AM315-10M, MU315-UC, AX315-YCD 6ml, 10ml, 1ml 14, 25, 46, 89, 187

Cytokeratin Cocktail AE1 & AE3 AM071-5M, AM071-10M, AX071-YCD, MU071-UC 6ml, 10ml, 16 ml, 1ml 14, 25, 46, 89, 187

Cytokeratin cocktail, Broad Spectrum 34βE12/C51/AE1 AM273-5M, AX273-YCD 6ml, 16 ml 14, 46, 90, 187

Cytokeratin cocktail, Broad SpectrumLL002+DEK-10+RCK108+OV-TL12/30+C11

AM372-5M, AM372-10M, AX372-YCD, MU372-UC 6ml, 10ml, 16 ml, 1ml 14, 25, 46, 90, 187

Cytokeratin, High MW 34βE12 AM291-5M, AM291-10M, AX291-YCD 6ml, 10ml, 16 ml 14, 25, 46, 90, 187

Cytokeratin, High MW (Basic) AE3 AM133-5M, AX133-YCD, MU133-UC 6ml, 16 ml, 1ml 14, 46, 90, 187

Cytokeratin, Low MW AE1 AM075-5M, AM075-10M, AX075-YCD, MU075-UC 6ml, 10ml, 16 ml, 1ml 14, 25, 46, 91, 187

Cytokeratin, Pan Lu-5 AM181-5M, AX181-YCD, MU181-UC 6ml, 16 ml, 1ml 14, 46, 91, 187

Cytokeratin, Pan C11 AM357-5M, AX357-YCD, MU357-UC 6ml, 16 ml, 1ml 14, 46, 91

Cytomegalovirus (CMV) BM204 AM254-5ME, MU254-UCE 6ml, 1ml 46, 91

Cytokeratin, High MW 34βE12 AM291-5M, AM291-10M, AX291-YCD 6ml, 10ml, 16 ml 14, 25, 46, 90, 187

DDAB 2-Component Pack - HK542-XAK 1000 Slides 178

Desmin D33 AM072-5M, AM072-10M, AX072-YCD, MU072-UC 6 ml, 10 ml, 16 ml, 1 ml

14, 28, 46, 92,187

Diastase (a-Amylase) - EK004-5K 200 Slides 30, 38

DOG1 1.1 AM570-5M, AM570-10M, AX570-YCD, MU570-UC 6 ml, 10 ml, 16 ml, 1 ml

14, 25, 46, 92, 187

Dystrophin Dys1 (Dy4/6D3) AM243-5M, AX243-YCD 6ml, 16 ml 14, 46, 92, 187

Dystrophin Dys2 (Dy8/6C5) AM244-5M, AX244-YCD 6ml, 16 ml 14, 47, 92, 187

200


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EEBER Probe - PR205-100E, PR205-YADE 0.650 ml, 0.650 ml 19, 150, 151

E-Cadherin EP6 AN725-5M, AN725-10M, AY725-YCD, NU725-UC 6 ml, 10 ml, 16mL, 1mL

14, 25, 47, 93, 187

E-Cadherin 36 AM390-5M, AM390-10M, AX390-YCD, MU390-UC 6ml, 10ml, 16 ml, 1ml 14, 25, 47, 93

eFISH 19q13/19p13 Dual Color Probe - FP045-10XE, FP045-20XE 10 Tests, 20 Tests 162

eFISH 1p36/1q25 Dual Color Probe - FP044-10XE, FP044-20XE 10 Tests, 20 Tests 162

eFISH ALK Dual Color Break Apart Probe - FP056-10XE, FP056-20XE 10 Tests, 20 Tests 162

eFISH AML1/ETO Dual Color Dual Fusion Probe - FP072-10XE, FP072-20XE 10 Tests, 20 Tests 162

eFISH BCL2/IGH Dual Color Dual Fusion Probe - FP074-10XE, FP074-20XE 10 Tests, 20 Tests 162

eFISH BCR/ABL Dual Color Dual Fusion Probe - FP071-10XE , FP071-20XE 10 Tests, 20 Tests 162

eFISH BIRC3/MALT1 Dual Color Dual Fusion Probe - FP075-10XE, FP075-20XE 10 Tests, 20 Tests 162

eFISH CCND1 Dual Color Break Apart Probe - FP069-10XE , FP069-20XE 10 Tests, 20 Tests 162

eFISH CCND1/CEN 11 Dual Color Probe - FP063-10XE, FP063-20XE 10 Tests, 20 Tests 162

eFISH CCND1/IGH Dual Color Dual Fusion Probe - FP057-10XE, FP057-20XE 10 Tests, 20 Tests 162

eFISH CEN 17/HER2 Dual Color Probe - FP039-10XE, FP039-20XE 10 Tests, 20 Tests 162

eFISH CHOP Dual Color Break Apart Probe - FP050-10XE, FP050-20XE 10 Tests, 20 Tests 162

eFISH CMYC Dual Color Break Apart Probe - FP064-10XE, FP064-20XE 10 Tests, 20 Tests 162

eFISH CMYC/CEN 8 Dual Color Probe - FP065-10XE, FP065-20XE 10 Tests, 20 Tests 162

eFISH CMYC/IGH Dual Color Dual Fusion Probe - FP067-10XE, FP067-20XE 10 Tests, 20 Tests 162

eFISH COL1A1 Dual Color Break Apart Probe - FP054-10XE, FP054-20XE 10 Tests, 20 Tests 162

eFISH COL1A1/PDGFB Dual Color Dual Fusion Probe - FP052-10XE, FP052-20XE 10 Tests, 20 Tests 162

eFISH Cyto Kit - DF510-20XE 20 Tests 163

eFISH EGFR/CEN 7 Dual Color Probe - FP040-10XE, FP040-20XE 10 Tests, 20 Tests 162

eFISH EGR1/5p15 Dual Color Probe - FP068-10XE, FP068-20XE 10 Tests, 20 Tests 162

eFISH ETV6/RUNX1 Dual Color Dual Fusion Probe - FP076-10XE, FP076-20XE 10 Tests, 20 Tests 162

eFISH EWSR1 Dual Color Break Apart Probe - FP048-10XE, FP048-20XE 10 Tests, 20 Tests 162

eFISH FGFR1/CEN 8 Dual Color Probe - FP042-10XE, FP042-20XE 10 Tests, 20 Tests 162

eFISH FGFR2/CEN 10 Dual Color Probe - FP055-10XE, FP055-20XE 10 Tests, 20 Tests 162

eFISH FUS Dual Color Break Apart Probe - FP058-10XE, FP058-20XE 10 Tests, 20 Tests 162

eFISH Histo Kit - DF500-20XE 20 Tests 163

eFISH IGH Dual Color Break Apart Probe - FP070-10XE , FP070-20XE 10 Tests, 20 Tests 162

eFISH MDM2/CEN 12 Dual Color Probe - FP038-10XE, FP038-20XE 10 Tests, 20 Tests 162

eFISH MET/CEN 7 Dual Color Probe - FP047-10XE, FP047-20XE 10 Tests, 20 Tests 162

eFISH NMYC/2q11 Dual Color Probe - FP043-10XE, FP043-20XE 10 Tests, 20 Tests 162

eFISH p16/CEN 9 Dual Color Probe - FP041-10XE , FP041-20XE 10 Tests, 20 Tests 162

eFISH PDGFB Dual Color Break Apart Probe - FP053-10XE , FP053-20XE 10 Tests, 20 Tests 162

eFISH PIK3CA/CEN 3 Dual Color Probe - FP059-10XE, FP059-20XE 10 Tests, 20 Tests 162

eFISH PML/RARA Dual Color Dual Fusion Probe - FP073-10XE , FP073-20XE 10 Tests, 20 Tests 162

eFISH RET Dual Color Break Apart Probe - FP061-10XE, FP061-20XE 10 Tests, 20 Tests 162

eFISH ROS1 Dual Color Break Apart Probe - FP060-10XE, FP060-20XE 10 Tests, 20 Tests 162

eFISH SYT Dual Color Break Apart Probe - FP049-10XE, FP049-20XE 10 Tests, 20 Tests 162

eFISH TERT/5q31 Dual Color Probe - FP066-10XE, FP066-20XE 10 Tests, 20 Tests 162

eFISH TFE3 Dual Color Break Apart Probe - FP051-10XE , FP051-20XE 10 Tests, 20 Tests 162

eFISH TP53/CEN 17 Dual Color Probe - FP062-10XE, FP062-20XE 10 Tests, 20 Tests 162

eFISH U Kit - DF530-40KE 40 Tests 163

eFISH VHL/CEN 3 Dual Color Probe - FP046-10XE, FP046-20XE 10 Tests, 20 Tests 162

EGFR Polyclonal AR335-5RE, AW335-YCDE, PU335-UPE 6ml, 16ml, 1ml 14, 47, 93

EGFR(PAN) EP38Y AN473-5ME, AN473-10ME, AX473-YCDE, NU473-UCE

6 ml, 10 ml, 16 ml, 1 ml 14, 25, 47, 93

201


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Elastic Stain - SS029-50X 50 Slides 169, 173

Elegance Red Substrate Pack - HK144-5KE 500 Slides 179

Empty Reagent Vials for Xmatrx Rx-Brown - XT078-V24 1each 21

Empty RFID Vial User Defined for ISH - XT079-PR0050 to XT079-PR0099 1each 20

Enhanced Antibody Diluent - HK941-YAKE 100ml 176

Ep-CAM E144 AN489-5M, AN489-10M, AX489-YCD, NU489-UC 6 ml, 10 ml, 16 ml, 1 ml

14, 25, 47, 94, 188

Epithelial Membrane Antigen (EMA) E29 AM057-5M, AM057-10M, AX057-YCD, MU057-UC 6ml, 10ml, 16 ml, 1ml 14, 25, 47, 94, 187

Epithelial Membrane Antigen (EMA) Mc5 AM182-5M, AM182-10M, AX182-YCD, MU182-UC 6ml, 10ml, 16 ml, 1ml 14, 25, 47, 94, 187

Epithelial-Specific Antigen MOC-31 AM316-5M, AM316-10M, MU316-UC, AX316-YCD 6ml, 10ml, 16 ml, 1ml 14, 25, 47, 94, 188

Epstein-Barr Virus Early Antigen 1108-1 AM222-5ME, MU222-UCE 6ml, 16ml, 1ml 47, 95, 188

ErbB-2/HER-2 EP1045Y AN471-5ME, AN471-10ME, AX471-YCDE 6 ml, 10 ml, 16 mL 13, 24, 47, 95

Estradiol Polyclonal AR038-5R, AR038-5RSG, AW038-YCD 6ml, 1ml, 16 ml 14, 47, 95, 188

Estrogen Receptor 1D5 AM272-2ME, AM272-10ME, AX272-YCDE, MU272-UCE 3ml, 10ml, 16ml, 1ml 14, 25, 47, 95,

188

Estrogen Receptor (ER Alpha) EPR703(2) AN509-5ME, AN509-10ME, AX509-YCDE, NU509-UCE

6 ml, 10 ml, 16 ml, 1 ml 14, 25, 47, 96

Estrogen Receptor (InSite® ER) ER88 AM368-5ME, AM368-10ME, AX368-YCDE, MU368-UCE 6ml, 10ml, 16 ml, 1ml 14, 25, 47, 96,

188

Estrogen Receptor-Alpha EP1 AN710-5ME, AN710-10ME, AY710-YCDE, NU710-UCE 6ml, 10 ml,16ml, 1ml 14, 25, 47, 96

Estrogen Receptor-Beta (ER-β) Polyclonal AR385-5R,AR385-10R, AW385-YCD, PU385-UP 6ml , 10ml , 16 ml, 1ml 14, 25, 47, 96

EZ-AR Common - HK545-XOK 1L 30, 35

EZ-AR1 Elegance, RTU - HK546-XAK 1L 34

EZ-AR1, RTU - HK521-XAK , HK521-XIK 1L, 2 Gallons 30, 35

EZ-AR2 Elegance, RTU - HK547-XAK 1L 34

EZ-AR2, RTU - HK522-XAK , HK522-XIK 1L, 2 Gallons 30, 35

EZ-AR3 - HK543-YOK 500 ml 30, 35

EZ-AR4 - HK544-YOK 500 ml 30, 35

EZ-AR™ 1 for Xmatrx - HX031-YCDE, HX031-YCX 200 test, 200 test 19




EZ-DeWax Solution, Conc - HK584-5KE 500 ml 29, 32

FFactor VIII-Related antigen F8 2.2.9 AM016-5M, AM016-10M, AX016-YCD, MU016-UC 6ml, 10ml, 16 ml, 1ml 14, 25, 47, 97,

188

Factor XIII Subunit A E980.1 AM337-5M, AM337-10M, AX337-YCD, MU337-UC 6ml, 10ml, 16 ml, 1ml 14, 25, 47, 97, 188

Factor XIII A EP3372 AN516-5M, AN516-10M, AX516-YCD, NU516-UC 6 ml, 10 ml, 16 ml, 1 ml

14, 25, 47, 97, 188

Fascin FCN01 AM488-5M, AM488-10M, AX488-YCD, MU488-UC 6 ml, 10 ml, 16 ml, 1 ml

14, 25, 47, 97, 188

Fast Red Substrate Pack - HK182-5KE 500 Slides 179

Fibronectin F14 AN569-5M, AN569-10M, AX569-YCD, NU569-UC 6 ml, 10 ml, 16 ml, 1 ml

14, 25, 47, 98, 188

FISH Consumable Kit, for Xmatrx - XT147-YAD 100 slides 20, 22

Follicle Stimulating Hormone (FSH) Polyclonal AR026-5R, AW026-YCD 6ml, 16 ml 14, 47

GGastrin Polyclonal AR019-5R, AW019-YCD, PU019-UP 6ml, 16 ml, 1ml 14, 47, 98, 188

GCDFP-15 EP1582Y AN481-5M, AN481-10M, AX481-YCD, NU481-UC 6 ml, 10 ml, 16 ml, 1 ml

14, 25, 47, 98, 188

Geimsa Stain - SS049-50X, SS049-YAK 50 Slides, 50 Slides 169, 173, 174

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GLEPP-1 5C11 AM336-5M, AX336-YCD, MU336-UC 6ml, 16 ml, 1ml 14, 47, 99

Glial Fibrillary Acidic Protein (GFAP) GA-5 AM020-5M, AM020-10M, AX020-YCD, MU020-UC 6ml, 10ml, 16 ml, 1ml 14, 25, 47, 99, 188

Glial Fibrillary Acidic Protein (GFAP) Polyclonal AR020-5R, AR020-10R, AW020-YCD, PU020-UP 6ml, 10ml, 16 ml, 1ml 14, 47, 99, 188

Glucagon Polyclonal AR039-5R, AW039-YCD, PU039-UP 6ml, 16 ml, 1ml 14, 47, 99, 188

Glut-1 SPM498 AM505-5M, AM505-10M, AX505-YCD, MU505-UC 6 ml, 10 ml, 16 ml, 1 ml

14, 25, 47, 100, 188

Glutathione S-Transferase Pi (GST Pi) Polyclonal AR249-5R,AW249-YCD, PU249-UP 6ml, 16 ml, 1ml 14, 47, 100, 188

Glypican-3 (GPC3) GPC3-88 AM539-5M, AM539-10M, MU539-UC 6 ml, 10 ml, 1 ml 25, 47, 100, 188

GMS for Fungi Stain Kit - SS042-50X, SS042-YAK 50 Slides, 50 Slides 170, 173, 174

Goat, SS Neg Control - HK406-5G 3 ml 179

Gomoris Trichome Blue - SS033-50X, SS033-YAK 50 Slides, 50 Slides 169, 173, 174

Gomoris Trichome Greeen - SS034-50X, SS034-YAK 50 Slides, 50 Slides 170, 173, 174

Gram Stain Kit - SS037-50X, SS037-YAK 50 Slides, 50 Slides 170, 173, 174

Granulocyte BM-2 MU210-UC 1ml 47, 100, 188

HH & E Retrieval Solution (10X) - HK169-5K 100 ml 37

Heat Shock Protein (HSP-70) BRM-22 AM289-5M, AX289-YCD, MU289-UC 6ml, 16 ml, 1ml 15, 47, 101, 188

Heat Shock Protein 27 (HSP 27) G3.1 AM171-5M, AM171-10M, AX171-YCD, MU171-UC 6ml, 10ml, 16 ml, 1ml 13, 26, 47, 101, 188

Helicobacter pylori Polyclonal AR442-5RE,AR442-10RE, AW442-YCDE, PU442-UPE 6ml, 16ml, 1ml 15, 26, 47, 101

Hematoxylin - HK100-5K, HK100-9K 6ml, 250 ml 180

Hemoglobin A Polyclonal AR021-5R,AW021-YCD 6ml, 16 ml 15, 47, 102, 188

Hepatitis B Virus Core Antigen (HBcAg) Polyclonal AR082-5RE, PU082-UPE 6ml, 1ml 47, 102

Herpes Simplex Virus Type I (HSV I) Polyclonal AR084-5RE, AW084-YCDE, PU084-UPE 6ml, 16ml, 1ml 15, 47, 102

Herpes Simplex Virus Type II (HSV II) Polyclonal AR085-5RE, AW085-YCDE, PU085-UPE 6ml, 16ml, 1ml 15, 47, 102

HGH Polyclonal AR707-5R, AR707-10R, AW707-YCD, PU707-UP 6ml, 10 ml, 16 ml, 1ml 15, 26, 47, 101, 188

HLA-DR LN3 AM154-5ME, AX154-YCDE, MU154-UCE 6ml, 16 ml, 1ml 15, 47, 103, 188

HPV 14 Probe - PR251-100E, PR251-YADE 0.650 ml, 0.650 ml 19, 150, 151

HPV 16/18 Probe - PR250-100E, PR250-YADE 0.650 ml, 0.650 ml 19, 150, 151

HSA HSA/E8 AM550-5M, AM550-10M, AX550-YCD, MU550-UC 6 ml, 10 ml, 16 ml, 1 ml

15, 25, 48, 103, 188

Hsa-miR-1 Probe - HM001-100E 0.650 ml 152, 157

Hsa-miR-106a Probe - HM106A-100E 0.650 ml 153, 157

Hsa-miR-10b Probe - HM010B-100E 0.650 ml 152, 157









Hsa-miR-151a-3p Probe - HM151-100E 0.650 ml 154, 157






Hsa-miR-200a Probe - HM200B-100E 0.650 ml 155, 157



203


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Ii500 S Bar Code Labeling System - BLS500 1 Unit 10, 30

i6000 Diagnostic - AS6030 1 Unit 8, 23

i6000 Infinity - AS6040 1 Unit 9, 23

i6000 Pipette Tips (192 tips) - XT105-01X 192 tips 20, 22, 29

i6000 Pipette Tips (960 tips) - XT104-05X 960 tips 20, 22, 29

IgA Polyclonal AR045-5R,AW045-YCD, PU045-UP 6ml, 16 ml, 1ml 15, 48, 103

IgD Polyclonal AR440-5R, AW440-YCD, PU440-UP 6ml, 16ml, 1ml 15, 48, 104, 188

IgG IgG88 AM367-5M, AX367-YCD, MU367-UC 6ml, 16 ml, 1ml 15, 48, 104, 188

IgG Polyclonal AR050-5R,AW050-YCD, PU050-UP 6ml, 16 ml, 1ml 15, 48, 104, 188

IgM IgM88 AM366-5M, AX366-YCD, MU366-UC 6ml, 16 ml, 1ml 15, 48, 104, 188

IgM Polyclonal AR427-5R, AR427-10R, AW427-YCD, PU427-UP 6ml, 10ml, 16 ml, 1ml 15, 48, 105, 188

Inhibin-Alpha R1 AM446-5M, AM446-10M, AX446-YCD, MU446-UC 6ml, 10ml, 16 ml, 1ml 15, 26, 48, 105, 189

Insulin HB125 AM029-5M, AW029-YCD, AX029-YCD, MU029-UC 6ml, 16 ml, 16 ml, 1ml 15, 48, 105, 189

Insulin EP125 AN735-5M, AN735-10M, AY735-YCD, NU735-UC 6 ml, 10 ml, 16mL, 1mL

15, 26, 48, 106, 189

Iron Stain Kit - SS030-50X, SS030-YAK 50 Slides, 50 Slides 170, 173, 174

ISH Consumable Kit, for Xmatrx - XT144-YAD 100 slides 20

JJ Chain JC88 AM374-5M, AX374-YCD, MU374-UC 6ml, 16 ml, 1ml 15, 48, 106

Jones' Basement Membrane Stain - SS058-50X, SS058-YAK 50 Slides, 50 Slides 170, 173, 174

KKappa Light Chain L1C1 AM048-5M, AM048-10M, AX048-YCD, MU048-UC 6ml, 10ml, 16 ml, 1ml 15, 26, 48, 106,

189

Kappa Light Chain K88 AM369-5M, AM369-10M, AX369-YCD, MU369-UC 6ml, 10ml, 16 ml, 1ml 15, 26, 48, 106, 189

Kappa Probe - PR214-100E, PR214-YADE 0.650 ml, 0.650 ml 19, 151

Ki-67 EP5 AN727-5M, AN727-10M, AY727-YCD, NU727-UC 6 ml, 10 ml, 16mL, 1mL

14, 25, 48, 95, 188

Ki-67 K-2 AM410-5M, AM410-10M, AX410-YCD, MU410-UC 6ml, 10ml, 16 ml, 1ml 15, 26, 48, 106, 189

Ki-67 EPR3611 AN527-5M, AN527-10M, AX527-YCD, NU527-UC 6 ml, 10 ml, 16 ml, 1 ml

15, 26, 48, 107, 189

Ki-67 and Lambda Light Chain Cocktail Mouse K-2 + Rabbit Poly AC562-5M, AC562-10M, AC562-YCD 6 ml, 10 ml, 16 ml 15, 26, 146, 189

Ki-67 Antigen, Proliferating Cell Ki88 AM370-5M, AM370-10M, AX370-YCD, MU370-UC 6ml, 10ml, 16 ml, 1ml 15, 26, 48, 107, 189

Ki-67 Antigen, Proliferating Cell MIB-1 AM297-5M, AM297-10M, AX297-YCD, MU297-UC 6ml, 10ml, 16 ml, 1ml 15, 26, 48, 107, 189

LLabel Roll i500 PLUS - XT035-XBX 1each 30

Lambda Light Chain Polyclonal AR049-5R,AW049-YCD, PU049-UP 6ml, 16 ml, 1ml 15, 48, 108, 189

Lambda Light Chain EP172 AN715-5M, AN715-10M, AY715-YCD, NU715-UC 6 ml, 10 ml, 16mL, 1mL

15, 26, 48, 108, 189

Lambda Probe - PR215-100E, PR215-YADE 0.650 ml, 0.650 ml 19, 151

Laminin Polyclonal AR078-5R, AR078-10R, AW078-YCD, PU078-UP 6ml, 10ml, 16 ml, 1ml 15, 26, 48, 108, 189

204


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Link Diluent - HK165-5KE 100 ml 176

Liquid DAB Chromogen - HK124-7K, HK124-9K 4 ml, 10ml 178

Lysozyme Polyclonal AR024-5R,AR024-10R, AW024-YCD, PU024-UP 6ml,10ml, 16 ml, 1ml 15, 26, 48, 108, 189

MMacrophage LN5 AM165-5M, AX165-YCD, MU165-UC 6ml, 16 ml, 1ml 15, 48, 109, 189

Masson's Trichrome Kit - SS035-50X, SS035-YAK 50 Slides, 50 Slides 170, 173, 174

Mast Cell Tryptase AA1 AM419-5M, AM419-10M, AX419-YCD, MU419-UC 6ml, 10ml, 16 ml, 1ml 26, 48, 109, 189

Mega Multilink/HRP Kit - QP300-XAK 1000 Slides 141

Melan-A (MART-1) A103 AM361-5M, AM361-10M, AX361-YCD, MU361-UC 6ml, 10ml, 16 ml, 1ml 15, 26, 48, 109, 189

Melanoma HMB45 AM001-5M, AM001-10M, AX001-YCD, MU001A-UC 6ml, 10ml, 16 ml, 1ml 15, 26, 48, 109, 189

Melanoma gp100 gp100/D5 AM536-5M, AM536-10M, AX536-YCD, MU536-UC 6 ml, 10 ml, 16 ml, 1 ml

15, 26, 48, 110, 189

Melanoma-Associated Antigen NKI/C3 AM077-5M, AM077-10M, AX077-YCD, MU077-UC 6ml, 10ml, 16 ml, 1ml 15, 26, 48, 110, 189

Mesothelin 5B2 AM433-5M, AM433-10M, AX433-YCD, MU433-UC 6ml, 10ml, 16 ml, 1ml 15, 26, 48, 110, 189

Microwave AR Accessory Kit - MW001-HB 3 units 34

Microwave AR Accessory Kit - MW001-SU 1 unit 34

MiTF MiTF/A13 AM554-5M, AM554-10M, AX554-YCD, MU554-UC 6 ml, 10 ml, 16 ml, 1 ml

15, 26, 48, 110, 189

Mitochondrial Antigen 113-1 MU213-UC 1ml 48, 111

MLH1 ES05 AM703-5M, AM703-10M, AY703-YCD, MU703-UC 6ml, 10ml, 16 ml, 1ml 15, 26, 48, 111, 189

MMP-9 EP1255Y AN504-5M, AN504-10M, AX504-YCD, NU504-UC 6 ml, 10 ml, 16 ml, 1 ml

15, 26, 48, 111, 189

MSH2 SP46 AN743-5M, AN743-10M, AY743-YCD, NU743-UC 6ml , 10ml , 16mL, 1mL 15, 26, 48, 111

MSH6 2D4B5 AM454-5M, AM454-10M, AX454-YCD, MU454-UC 6 ml, 10 ml, 16 ml, 1 ml

15, 26, 48, 112 ,189

MUC4 1G8 AM455-5M, AM455-10M, AX455-YCD, MU455-UC 6 ml, 10 ml, 16 ml, 1 ml

15, 26, 48, 112, 189

MUC5AC 45M1 AM456-5M, AM456-10M, AX456-YCD, MU456-UC 6 ml, 10 ml, 16 ml, 1 ml

15, 26, 48, 112, 189

Mucicarmine Stain - SS036-50X, SS036-YAK 50 Slides, 50 Slides 171, 173, 174

Mucin 1 (MUC1) EPR1023 AN564-5M, AN564-10M, AX564-YCD, NU564-UC 6 ml, 10 ml, 16 ml, 1 ml

15, 26, 48, 112, 189

Mucin 2 (MUC2) CCP58 AM358-5M, AX358-YCD, MU358-UC 6ml, 16 ml, 1ml 15, 48, 113

Multi-Drug Resistance Marker (P-Glycoprotein) MDR88 AM391-5M, AX391-YCD, MU391-UC 6ml, 16 ml, 1ml 15, 48, 113

Muscle Actins Actin 88 Cocktail AM381-5M, AX381-YCD, MU381-UC 6ml, 16 ml, 1ml 15, 48, 113

Myelin Basic Protein MBP88 AM380-5M, AM380-10M, AX380-YCD 6ml, 10ml, 16 ml 15, 26, 48, 113

Myeloid specific Antigen BM-3 AM216-5M, AX216-YCD 6ml, 16 ml 16, 48, 114

Myeloid specific Antigen BM-1 AM164-5M, MU164-UC, AX164-YCD 6ml, 16 ml, 1ml 16, 48, 114, 189

Myeloperoxidase (MPO) Polyclonal AR496-5R, AR496-10R, AW496-YCD, PU496-UP 6 ml, 10 ml, 16 ml, 1 ml

16, 26, 49, 114, 189

Myf4 LO26 AM432-5M, AM432-10M, AX432-YCD, MU432-UC 6ml, 10ml, 16 ml, 1ml 16, 26, 49, 114, 189

Myoglobin MG-1 AM012-5M, AX012-YCD, MU012-UC 6ml, 16 ml, 1ml 16, 49, 115, 189

Myoglobin Polyclonal AR012-5R, AW012-YCD, PU012-UP 6ml, 16 ml, 1ml 16, 49, 115, 189

Myosin Heavy Chains, Smooth Muscle SMMS.1 AM331-5M, AM331-10M, AX331-YCD, MU331-UC 6ml, 10ml, 16 ml, 1ml 16, 26, 49, 115

Myosin,Skeletal Muscle MY-32 AM109-5M, AX109-YCD, MU109-UC 6ml, 16 ml, 1ml 16, 49, 115, 189

NNapsin A IP64 AM701-5M, AM701-10M, AY701-YCD, MU701-UC 6ml, 10ml, 16 ml, 1ml 16, 26, 49, 116,

189

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N-Cadherin EPR1792Y AN565-5M, AN565-10M, AX565-YCD, NU565-UC 6 ml, 10 ml, 16 ml, 1 ml

16, 26, 49, 116, 189

Neurofilament NE-14 AM073-5M, AM073-10M, AX073-YCD, MU073-UC 6ml, 10ml, 16 ml, 1ml 16, 26, 49, 116, 190

Neuron Specific Enolase (NSE) MIG-N3 AM055-5M, AM055-10M, AX055-YCD, MU055-UC 6ml, 10ml, 16 ml, 1ml 16, 26, 49, 116, 190

New Fuchsin Substrate Pack - HK183-5KE 400 Slides 179

NGF Receptor EP1039Y AN522-5M, AN522-10M, AX522-YCD, NU522-UC 6 ml, 10 ml, 16 ml, 1 ml

16, 26, 49, 117, 190

NGF Receptor EP31 AN738-5M, AN738-10M, AY738-YCD, NU738-UC 6 ml, 10 ml, 16mL, 1mL

16, 26, 49, 117, 190

Nordic Ware Pressure Cooker - NW001-PC 1 each 39

Nuclear Ribonucleoprotein 58-15 MU230-UC 1ml 49, 117, 190

OOct 2 EPR542 AN558-5M, AN558-10M, AX558-YCD, NU558-UC 6 ml, 10 ml, 16 ml,

1 ml16, 26, 49, 117, 190

Oct 4 EP143 AN724-5M, AN724-10M, AY724-YCD, NU724-UC 6 ml, 10 ml, 16ml, 1ml 16, 28, 49, 118, 190

Oil Red O Stain - SS043-50X, SS043-YAK 50 Slides, 50 Slides 169, 173, 174

Oligo d(T) Probe - PR217-100E, PR217-YADE 0.650 ml, 0.650 ml 19, 151

One-Step Polymer-HRP - QD620-YIKE 500 Slides 139

One-Step Polymer-HRP - QD630-XAKE 1000 Slides 139

Optimiser Reagent Vial (20mL) - XT026-601 to XT026-799, XT026-601P to XT026-660P 1 each, 1 each 28

Optimiser Reagent Vial (20mL) - XT026-PR601 to XT026-PR615 1 each 28

Optimiser Reagent Vial (20mL) - XT026-V24 24/pk 20, 22, 28

Optimiser Reagent Vials, 20mL - XT101-24X 24 pack 28

Optimiser Vial Holders (24) - XT027-H24 24/pk 28

OptiPlus Barrier Slides, 2/3 Test Area - XT013-CL, XT013-SL 1440 Slides, 72 Slides 29, 181

OptiPlus Barrier Slides, 3 X 1/3 Test Area,20 Boxes - XT014-CL, XT014-SL 1440 Slides, 72 Slides 29, 181

OptiPlus Microscope Slides - XT002-CL, XT002-SL 1440 Slides, 72 Slides 181

Osteonectin OST1 AM387-5M, AX387-YCD, MU387-UC 6ml, 16 ml, 1ml 16, 49, 118

Pp105 Proliferation-Associated Nuclear Antigen 2B3 AM317-5M, AX317-YCD, MU317-UC 6ml, 16 ml, 1ml 16, 49, 118,

p16 (INK4a) G175-405 AM540-5M, AM540-10M, MU540-UC 6 ml , 10 ml, 1 ml 26, 49, 118, 190

p16+Ki67 cocktail Mouse G175-405 + Rabbit EPR3611 AC601-5M, AC601-10M 6 ml, 10ml 26, 146, 190

p21/WAF1 4D10 AM434-5M, AX434-YCD, MU434-UC 6ml, 16 ml, 1ml 16, 49, 119, 190

p27 (Kip1) Y236 AN492-5M, AN492-10M, AX492-YCD, NU492-UC 6 ml, 10 ml, 16 ml, 1 ml

16, 26, 49, 119, 190

p27 (Kip1) DCS72 AM396-5M, AX396-YCD, MU396-UC 6ml, 16 ml, 1ml 16, 49, 119

p34cdc2 (Cyclin Dependent Kinase) POH-1 AM301-5M, AX301-YCD, MU301-UC 6ml, 16 ml, 1ml 16, 49, 119, 190

P504S (AMACR) 13H4 AN449-5ME, AN449-10ME, AX449-YCDE, NU449-UCE 6ml, 10ml, 16ml, 1ml 16, 26, 49, 120,

190

P504S (AMACR) RBT-AMACR AN538-5M, AN538-10M, AX538-YCD, NU538-UC 6 ml, 10 ml, 16 ml, 1 ml

16, 26, 49, 120, 190

p53 EP9 AN728-5M, AN728-10M, AY728-YCD, NU728-UC 6 ml, 10 ml, 16mL, 1mL

16, 26, 49, 120, 190

p53 Protein BP53-12-1 AM195-5M, AM195-10M, AX195-YCD, MU195-UC 6ml, 10ml, 16 ml, 1ml 16, 26, 49, 120, 190

p53 Protein DO7 AM239-5M, AM239-10M, AX239-YCD, MU239-UC 6ml, 10ml, 16 ml, 1ml 16, 27, 49, 121, 190

p53 Protein 1801 AM240-5M, AM240-10M, AX240-YCD, MU240-UC 6ml, 10ml, 16 ml, 1ml 16, 27, 49, 121, 190

p63 Protein 4A4 AM418-5M, AM418-10M, AX418-YCD, MU418-UC 6ml, 10ml, 16 ml, 1ml 16, 27, 49, 121, 190

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p63 + CK5 Mouse 4A4 + Rabbit EPR1600Y AC602-5M, AC602-10M, AC602-YCD 6ml, 10ml, 16ml 16, 27, 147

PAP A40010 AM532-5M, AM532-10M, AX532-YCD, MU532-UC 6 ml, 10 ml, 16 ml, 1 ml

16, 27, 49, 121, 190

PAP Pen - XT001-PP 1 each 29, 181

Papillomavirus Type 16 (HPV-16) Cam Vir-1 AM362-5ME, AX362-YCDE, MU362-UCE 6ml, 16 ml, 1ml 16, 49, 121

PAS for Fungi Stain - SS053-50X, SS053-YAK 50 Slides, 50 Slides 171, 173, 174

PAS Staining Kit - SS032-50X, SS032-YAK 50 Slides, 50 Slides 171, 173, 174

PAX-5 ZP007 AM457-5M, AM457-10M, AX457-YCD, MU457-UC 6 ml, 10 ml, 16 ml, 1 ml

16, 27, 49, 122, 190

Paxillin (N-Term) Y113 AN521-5M, AN521-10M, AX521-YCD, NU521-UC 6 ml, 10 ml, 16 ml, 1 ml

16, 27, 49, 122, 190

PDCD4 EPR3432 AN524-5M, AN524-10M, AX524-YCD, NU524-UC 6 ml, 10 ml, 16 ml, 1 ml

16, 27, 49, 122, 190

PDGF Receptor-ß Y92 AN463-5M, AN463-10M, AX463-YCD, NU463-UC 6 ml, 10 ml, 16 ml, 1ml 16, 27, 49, 122, 190

Pepsin 4-Pack Kit - EK000-5K 150 Slides 38

Pepsin 4-Pack Kit - EK000-10K, EK000-10X 200 Slides, 200 Slides 19, 30

Perodic Acid Schiff (PAS) Diastase Stain - SS039-50X, SS039-YAK 50 Slides, 50 Slides 171, 173, 174

Peroxide Block - HK111-5K, HK111-50K 6ml, 50ml 177

PGP9.5 3D9 AM736-5ME, AM736-10ME, AY736-YCDE, MU736-UCE 6ml , 10ml, 16 ml, 1ml 16, 27, 49, 123

Phosphate Buffer Saline (PBS) - HK091-5KE 120 ml 176

PIN4 (p63+CKHMW+P504S)Mouse 4A4+ Mouse34BE12+ Rabbit13H4

AM448-5ME, AM448-10ME, AX448-YCDE, MU448-UCE 6ml, 10ml, 16ml, 1ml 16, 27, 147,190

Pipette Tips, 200ul (192 tips), for Xmatrx - XT146-01X 960 tips 21, 22

Pipette Tips, 200ul (960 tips)/5 Boxes, for Xmatrx - XT145-05X 960 tips 21, 22

Placental Alkaline Phosphatase (PLAP) PL8-F6 AM228-5M, AM228-10M, AX228-YCD, MU228-UC 6ml, 10ml, 16 ml, 1ml 16, 27, 49, 123, 190

Placental Lactogen (hPL) Polyclonal AR040-5R,AR040-10R, AW040-YCD, PU040-UP 6ml, 10ml, 16 ml, 1ml 16, 27, 49, 123, 190

Platelet-Derived Growth Factor (PDGF) PDGF88 AM376-5M, AX376-YCD, MU376-UC 6ml, 16 ml, 1ml 16, 49, 123

Platelet-Derived Growth Factor (PDGF) Polyclonal AR376-5R, AW376-YCD, PU376-UP 6ml,16 ml, 1ml 16, 49, 124

PMS2 EPR3947 AN577-5M, AN577-10M, AX577-YCD, NU577-UC 6 ml, 10 ml, 16 ml, 1 ml

16, 27, 49, 124, 190

PMSA EP192 AN714-5M, AN714-10M, AY714-YCD, NU714-UC 6 ml, 10 ml, 16mL, 1mL

17, 27, 50, 126, 190

Positive Control on Barrier Slides - FB-001M to FB-743N 5/PK 184 - 191

Positive Control Slides - FG-001M to FG-743N 5/PK 184 - 191

Power Block (10X) - HK085-5KE 100 ml 177

Power Block Reagent, Ready-to-use - BS-1310-25 25ml 177

Progesterone Receptor EP2 AN711-5ME, AN711-10ME, AY711-YCDE, NU711-UCE 6ml, 10ml, 16 ml, 1ml 16, 27, 49, 124

Progesterone Receptor 1A6 AM172-2ME, AM172-10ME, AX172-YCDE, MU172-UCE 3ml, 10ml, 16ml, 1ml 16, 27, 49, 124

Progesterone Receptor (InSite®PR) PR88 AM328-5ME, AM328-10ME, AX328-YCDE, MU328-UCE 6ml, 10ml, 16 ml, 1ml 16, 27, 49, 125,

190

Prolactin ME.121 AM031-5M, AM031-10M, AX031-YCD, MU031-UC 6ml, 10ml, 16 ml, 1ml 16, 28, 49, 125

Proliferating Cell Nuclear Antigen (PCNA) PC10 AM252-5M, AM252-10M, AX252-YCD, MU252-UC 6ml, 10ml, 16 ml, 1ml 16, 27, 49, 125, 190

Prostate Specific Acid Phosphatase (PSAP) B01-94-21M-NA AM013-5ME, AM013-10ME, AX013-YCDE, MU013-UCE 6ml, 10ml, 16 ml, 1ml 16, 27, 50, 125,

190

Prostate Specific Antigen(PSA) ErPr8 AM014-5ME, AM014-10ME, AX014-YCDE, MU014-UCE 6ml , 10ml , 16 ml, 1ml 16, 27, 50, 126,

190

Protease XXIV 4-Pack Kit - EK002-5K 150 Slides 38

Protease XXIV 4-Pack Kit - EK002-10K, EK002-10X 200 Slides, 200 Slides 19, 30

pS2 Estrogen Inducible Protein PS2.1 AM190-5M, AM190-10M, AX190-YCD, MU190-UC 6ml, 10ml, 16 ml, 1ml 17, 27, 50, 126, 190

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RRabbit, SS Neg Control - HK408-5R, HK408-7R 3 ml, 17 ml 179

Rat, SS Neg Control - HK407-5T 3 ml 179

RCC (Renal Cell Carcinoma) RCC-26 AM543-5M, AM543-10M, AX543-YCD, MU543-UC 6 ml, 10 ml, 16 ml, 1 ml

17, 27, 50, 126, 190

Reagent Vial Insert for RFID - XT149-V24 24/PK 21, 22

Renal Masson's Trichome - SS050-50X, SS050-YAK 50 Slides, 50 Slides 171, 173, 174

Resin Ribbon i500 PLUS - XT034-XEX 1each 30

Reticulin/No Counterstain Stain Kit - SS046-50X, SS046-YAK 50 Slides, 50 Slides 172, 173, 174

Reticulin/No Counterstain Stain Kit - SS047-50X, SS047-YAK 50 Slides, 50 Slides 172, 173, 174

Retinoblastoma (RB) Probe - PR225-100E, PR225-YADE 0.650 ml, 0.650 ml 19, 151

SS100 Beta EP32 AN713-5M, AN713-10M, AY713-YCD, NU713-UC 6 ml, 10 ml, 16mL,

1mL17, 27, 50, 127, 190

S-100 Protein 15E2E2 AM058-5M, AM058-10M, AX058-YCD, MU058-UC 6ml, 10ml, 16ml, 1ml 17, 27, 50, 127, 190

S-100 Protein Polyclonal AR058-5R, AR058-10R, AW058-YCD, PU058-UP 6ml, 10ml, 16 ml, 16 ml, 1ml

17, 27, 50, 127, 190

S100P EP186 AN712-5M, AN712-10M, AY712-YCD, NU712-UC 6 ml, 10 ml, 16mL, 1mL

17, 27, 50, 127, 190

S-A HRP Diluent - HK157-5KE 100 ml 176

Safranin O Staining Protocol - SS040-50X, SS040-YAK 50 Slides, 50 Slides 172, 173, 174

Sarcomeric Actin ZMSA-5 AM511-5M, AM511-10M, AX511-YCD, MU511-UC 6 ml , 1 ml, 16 ml, 1 ml 17, 27, 50, 128, 190

Scramble Probe - PR032-100E 0.650 ml 156, 157

Secretin Polyclonal AR067-5R, AR067-5RSG, AW067-YCD 6ml, 1ml, 16 ml 17, 50, 128, 191

Slide Barcode Labels - AM6010 TO AM7990 AR6010 TO AR6600 100/SHEET 29

SS Double Stain Polymer Detection Kit I/DAB & Fast Red - QS200-60K 60 Slides 144

SS Double Stain Polymer Detection Kit I/DAB & Fast Red - QS200-YADE 100 slides 18

SS Double Stain Polymer Detection Kit I/Large Volume - QS210-YIK 500 Slides 144

SS Double Stain Polymer Detection Kit II/DAB & Fast Red - QS400-60K 60 Slides 144

SS Double Stain Polymer Detection Kit II/DAB & Fast Red - QS400-YADE 100 slides 18

SS Double Stain Polymer Detection Kit II/Large Volume - QS410-YIK 500 Slides 144

SS Goat Link - HK337-5G 6ml 142

SS Mouse Link - HK335-5M, HK335-9M 6ml, 50 ml 142

SS Mouse Negative Control - HK119-7M 17 ml 179

SS Multilink - HK340-5K, HK340-9K 6ml, 50 ml 142

SS Multilink Kit,HRP/DAB - QD470-60K, QD470-YCX 60 Slides, 200 Slides 28, 141

SS One Step Polymer-HRP IHC Detection System for Xmatrx - QD610-YAXE 200 slides 28

SS One Step Polymer-HRP IHC Detection System for Xmatrx - QD610-YADE 200 Slides 18

SS Rabbit Link - HK336-5R, HK336-9R 6ml, 50 ml 142

SS Rat Link - HK338-5T 6ml 142

SS Strept Alka Phos Label - HK331-5K, HK331-9K 6ml, 50 ml 142

Stat 5-Alpha (C-term) E289 AN480-5M, AN480-10M, AX480-YCD, NU480-UC 6 ml , 10 ml, 16 ml, 1 ml

17, 27, 50, 128,191

Substance P Polyclonal AR069-5R, AW069-YCD, PU069-UP 6ml,16 ml, 1ml 17, 50, 128, 191

Sudan Black B Stain - SS041-50X, SS041-YAK 50 Slides, 50 Slides 172, 173, 174

Super Mount - HK079-5K, HK079-7K 15 ml, 50 ml 180

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Super Sensitive HRP Label - HK330-5K, HK330-9K 6ml, 50 ml 142

Super Sensitive Link HRP IHC Detection System - QP000-5L 50-60 Slides 141

Super Sensitive Link Label IHC Detection System, Alk Phos - QA900-9L 500 Slides 141

Super Sensitive Link Label IHC Detection System, AP - LA000-ULE 1000 Slides 141

Super Sensitive Link Label IHC Detection System, HRP/DAB - QD420-YIKE 500 Slides 139

Super Sensitive Link Label IHC Detection System, HRP/DAB Mega - QD430-XAKE 1000 Slides 139

Super Sensitive Non-Biotin HRP, DAB Automated Kit - QD410-YAXE 200 Slides 28

Super Sensitive Non-Biotin HRP, DAB Kit - QD400-60KE 60 Slides 139

Super Sensitive Link HRP IHC Detection System - QP900-9L 500 Slides 141

Super Sensitive One Step Polymer-HRP ISH Detection System - DF400-50KE 50 Slides 158

Super Sensitive One Step Polymer-HRP ISH Detection System - DF400-YADE 100 slides 19, 158

Super Sensitive Polymer-HRP IHC Kit - QD440-XAK 1000 Slides 139

Super Sensitive Wash Buffer, 20X - HK583-5KE 500 ml 30, 176

Survivin EP2880Y AN503-5M, AN503-10M, AX503-YCD, NU503-UC 6 ml, 10 ml, 16 ml, 1 ml

17, 27, 50, 129, 191

Synaptophysin Snp88 AM363-5M, AM363-10M, AX363-YCD, MU363-UC 6ml, 10ml, 16 ml, 1ml 17, 27, 50, 129, 191

TTau TAU-2 AM412-5M, AM412-10M, AX412-YCD, MU412-UC 6ml, 10ml, 16 ml, 1ml 17, 27, 50, 129,

191

Tau Tau-5 AM459-5M, AM459-10M, AX459-YCD, MU459-UC 6 ml, 10 ml, 16 ml, 1 ml

17, 27, 50, 129, 191

Terminal Deoxynucleotidyl Transferase (TdT) TdT88 AM373-5M, AM373-10M, AX373-YCD, MU373-UC 6ml, 10ml, 16 ml, 1ml 13, 27, 50, 130

Terminal Deoxynucleotidyl Transferase (TdT) Polyclonal AR373-5R, AW373-YCD 6ml, 16 ml 17, 50, 130, 191

TFPU.1 EPR3159Y AN520-5M, AN520-10M, AX520-YCD, NU520-UC 6 ml, 10 ml, 16 ml, 1 ml

17, 27, 52, 130, 191

Thyroglobulin 2H11 AM032-5M, AM032-10M, AX032-YCD, MU032-UC 6ml, 10ml, 16 ml, 1ml 17, 27, 50, 130, 191

Thyroid Stimulating Hormone (TSH) 5404 AM033-5M, AM033-10M, AX033-YCD, MU033-UC 6ml, 10ml, 16 ml, 1ml 17, 27, 50, 131

Thyroid Stimulating Hormone (TSH) Polyclonal AR033-5R, AW033-YCD 6ml, 16 ml 17, 50, 131

Thyroxine D5 AM034A-5M, AX034-YCD, MU034A-UC 6ml, 16 ml, 1ml 17, 50, 131, 191

TIA -1 2G9A10F5 AM529-5M, AM529-10M, AX529-YCD, MU529-UC 6 ml, 10 ml, 16 ml, 1 ml 17, 27, 50, 132

Toluidine blue stain - SS057-50X, SS057-YAK 50 Slides, 50 Slides 172, 173, 174

Topo 2 Alpha EP1102Y AN576-5M, AN576-10M, AX576-YCD, NU576-UC 6 ml, 10 ml, 16 ml, 1 ml

17, 27, 50, 132, 191

Toxoplasma gondii Polyclonal AR125-5RE, PU125-UPE 6ml, 1ml 50, 132

Transferrin HT1/13.6.3 AM025-5M, AX025-YCD 6ml, 16 ml 17, 50, 132, 191

Transforming Growth Factor (TGF),Alpha TGF88 AM377-5M, AX377-YCD, MU377-UC 6ml, 16 ml, 1ml 17, 50, 133

Tris Buffer, Conc pH 7.6 - HK098-5K 3/PK 176

Trypsin 4-Pack Kit - EK001-5K 150 Slides 38

Trypsin 4-Pack Kit - EK001-10K, EK001-10X 200 Slides, 200 Slides 19, 30

TTF-1 8G7G3/1 AM397-5M, AM397-10M, AX397-YCD, MU397-UC 6ml, 10ml, 16 ml, 1ml 17, 27, 50, 131, 191

TTF1 and Cytokeratin 5/6 cocktailMouse 8G7G3/1 + Rabbit EPR1600Y + Rabbit1602Y

AC563-5M, AC563-10M, AC563-YCD 6 ml, 10 ml, 16 ml 17, 27, 146, 191

TTF-1+CK20+CK7 cocktailMouse 8G7G3/1 + Rabbit EP1582Y + EP1619Y

AC605-5M, AC605-10M, AC605-YCD 6 ml, 16 ml 17, 27, 147

TTF-1+GCDFP-15 cocktail Mouse 8G7G3/1 + Rabbit EP1582Y AC604-5M, AC604-10M, AC604-YCD 6 ml, 16 ml 17, 27, 146, 191

209


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Tumor-Associated Glycoprotein (TAG-72) B72.3 AM054-5M, AM054-10M, AX054-YCD, MU054-UC 6ml, 10ml, 16 ml, 1ml 17, 27, 50, 133, 191

Tumor-Associated Glycoprotein (TAG-90 BCA) B6.2 AM005-5M, AX005-YCD, MU005-UC 6ml, 16 ml, 1ml 17, 50, 133, 191

Tyrosinase Ty/G5 AM535-5M, AM535-10M, AX535-YCD, MU535-UC 6 ml, 10 ml, 16 ml, 1 ml

17, 27, 50, 133, 191

UU6 Probe - PR031-100E 0.650 ml 156, 157

User Defined Empty RFID Tagged Vials Two Step IHC - XT077-V24 1each 21

User Defined Empty RFID Tagged Vials Two Step IHC - XT077-AX0801 to XT0821-AX0999 1 each 20

User Defined Empty RFID Tagged Vials Two Step IHC - XT077-AX0601 to XT077-AX0800 1 each 20

VVan Gieson Stain - SS044-50X, SS044-YAK 50 Slides, 50 Slides 172, 173, 174

VEGF Polyclonal AR483-5R, AR483-10R, AW483-YCD, PU483-UP 6 ml, 10 ml, 16 ml, 1 ml

17, 27, 50, 134, 191

Villin ID2C3 AM525-5M, AM525-10M, AX525-YCD, MU525-UC 6 ml, 10 ml, 16 ml, 1 ml

17, 27, 50, 134, 191

Vimentin V9 AM074-5M, AM074-10M, AX074-YCD, MU074-UC 6ml, 10ml, 16 ml, 1ml 17, 27, 50, 134, 191

Vimentin,Non-Hematopoietic LN6 AM163-5M, AX163-YCD, MU163-UC 6ml, 16 ml, 1ml 17, 50, 134, 191

VIP Polyclonal AR530-5R, AR530-10R, AW530-YCD, PU530-UP 6 ml, 10 ml, 16 ml, 1 ml

17, 27, 50, 135, 191

von Kossa stain - SS045-50K 50 Slides 173, 174

WWilm's Tumor CAN-R9(IHC)-56-2 AN584-5M, AN584-10M, AX584-YCD, NU584-UC 6 ml, 10 ml, 16 ml,

1 ml17, 27, 50, 135, 191

XX-DeWax - HX016-XAK, HX016-XEK 1000 ml, 1 Gallon 21

Xflex Consumable Kit, for Xmatrx - XT132-YCD 200 slides 20

Xflex Ultra Consumable Kit, for Xmatrx - XT148-YCD 200 slides 20

Xmatrx Elite - AS4040B 1 Unit 2, 7, 11

Xmatrx Infinity - AS4000RX 1 Unit 6, 11

Xmatrx Mini - AS1010 1 Unit 5, 7, 11

Xmatrx Nano - AS1000 1 Unit 4, 7, 11

Xmatrx Reagent Vials - XT126-24V 24-/PK 20, 22

Xmatrx Ultra AS4030B 1 Unit 3, 11

Xmount - HX035-04X, HX035-10X, HX035-40D 4 ml, 10 ml, 10 ml 21

XVIZ Detection Kit - QD550-YCDE, QD550-YCX 200 slides, 200 slides 18

X-Wash Buffer, 20X, for Xmatrx - HX020-YIK 500 ml 21

X-Wash ISH Wash Buffer - HX017-YIK 500 ml 21

ZZAP-70 YE291 AN466-5M, AN466-10M, AX466-YCD, NU466-UC 6 ml, 10 ml, 16 ml, 1ml 17, 27, 50, 135,

191

ZAP-70 ZAP70-C3 AM544-5M, AM544-10M, AX544-YCD, MU544-UC 6 ml, 10 ml, 16 ml, 1 ml

17, 27, 50, 135, 191

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Catalog Number Index

www.biogenex.com

Cat. No. Product Name Page

AAC559-5M, AC559-10M, AC559-YCD CD3 and CD20 cocktail 12, 23, 145, 185

AC561-5M, AC561-10M, AC561-YCD CD10 and Cyclin D1 cocktail 12, 24, 145, 185

AC562-5M, AC562-10M, AC562-YCD

Ki-67 and Lambda Light Chain Cocktail 15, 26, 146, 189

AC563-5M, AC563-10M, AC563-YCD

TTF1 and Cytokeratin 5/6 cocktail 17, 27, 146, 191

AC595-5M, AC595-10M,AC595-YCD CD4 + C8 Cocktail 12, 23, 145

AC596-5M, AC596-10M, AC596-YCD CDX-2+CK7 cocktail 13, 24, 145, 186

AC597-5M, AC597-10M, AC597-YCD CDX-2+CK20+CK7 cocktail 13, 24, 147

AC601-5M, AC601-10M p16+Ki67 cocktail 26, 146, 190

AC602-5M, AC602-10M, AC602-YCD Mouse 4A4 + Rabbit EPR1600Y 16, 27, 147

AC604-5M,AC604-10M, AC604-YCD TTF-1+GCDFP-15 cocktail 17, 27, 146, 191

AC605-5M, AC605-10M, AC605-YCD TTF-1+CK20+CK7 cocktail 17, 27, 147

AM001-5M, AM001-10M, AX001-YCD, MU001A-UC Melanoma 15, 26, 48, 109,

189

AM005-5M, AX005-YCD, MU005-UC

Tumor-Associated Glycoprotein (TAG-90 BCA) 17, 50, 133, 191

AM008-5M, AM008-10M, AX008-YCD, MU008A-UC Alpha-Fetoprotein (AFP) 11, 23, 43, 53,

184

AM009-5M, AM009-10M, AX009-YCD, MU009-UC Carcinoembryonic Antigen (CEA) 11, 23, 43, 60,

185

AM012-5M, AX012-YCD, MU012-UC Myoglobin 16, 49, 115, 189

AM013-5ME, AM013-10ME, AX013-YCDE, MU013-UCE

Prostate Specific Acid Phosphatase (PSAP)

16, 27, 50, 125, 190


Prostate Specific Antigen(PSA) 16, 27, 50, 126, 190

AM016-5M, AM016-10M, AX016-YCD, MU016-UC Factor VIII-Related antigen 14, 25, 47, 97,

188

AM020-5M, AM020-10M, AX020-YCD, MU020-UC


14, 25, 47, 99, 188

AM025-5M, AX025-YCD Transferrin 17, 50, 132, 191

AM029-5M, AW029-YCD, AX029-YCD, MU029-UC Insulin 15, 48, 105, 189

AM031-5M, AM031-10M, AX031-YCD, MU031-UC Prolactin 16, 28, 49, 125

AM032-5M, AM032-10M, AX032-YCD, MU032-UC Thyroglobulin 17, 27, 50, 130,

191


Thyroid Stimulating Hormone (TSH) 17, 27, 50, 131

AM034A-5M, AX034-YCD, MU034A-UC Thyroxine 17, 50, 131, 191

AM048-5M, AM048-10M, AX048-YCD, MU048-UC Kappa Light Chain 15, 26, 48, 106,

189


Tumor-Associated Glycoprotein (TAG-72)

17, 27, 50, 133, 191

AM055-5M, AM055-10M, AX055-YCD, MU055-UC Neuron Specific Enolase (NSE) 16, 26, 49, 116,

190



14, 25, 47, 94, 187

AM058-5M, AM058-10M, AX058-YCD, MU058-UC S-100 Protein 17, 27, 50, 127,

190


AM059-5ME, AX059-YCDE, MU059-UCE Adenovirus 11, 43, 52

AM071-5M, AM071-10M, AX071-YCD, MU071-UC Cytokeratin Cocktail 14, 25, 46, 89,

187

AM072-5M, AM072-10M, AX072-YCD, MU072-UC Desmin 14, 28, 46,

92,187

AM073-5M, AM073-10M, AX073-YCD, MU073-UC Neurofilament 16, 26, 49, 116,

190

AM074-5M, AM074-10M, AX074-YCD, MU074-UC Vimentin 17, 27, 50, 134,

191

AM075-5M, AM075-10M, AX075-YCD, MU075-UC Cytokeratin, Low MW 14, 25, 46, 91,

187

AM077-5M, AM077-10M, AX077-YCD, MU077-UC Melanoma-Associated Antigen 15, 26, 48, 110,

189

AM090-5M, AM090-10M, AX090-YCD, MU090-UC Actin, Muscle-Specific 11, 23, 43, 51,

184

AM097-5M, AX097-YCD, MU097-UC Alpha-Actinin 11, 43, 52, 184

AM109-5M, AX109-YCD, MU109-UC Myosin,Skeletal Muscle 16, 49, 115, 189

AM111-5M, AM111-10M, AX111-YCD CD45 12, 24, 45, 72,

186

AM113-5M, AM113-10M, AX113-YCD, MU113-UC CD45RO (T Cell) 13, 24, 45, 74,

186

AM126-5M, AM126-10M, AX126-YCD, MU126-UC Chromogranin A 13, 24, 45, 82,

186

AM128-5M, AM128-10M, AX128-YCD, MU128-UC Actin, Smooth Muscle 11, 23, 43, 51,

184

AM131-5M, AM131-10M, AX131-YCD, MU131-UC Cytokeratin 8 & 18 14, 25, 46, 87,

187

AM132-5M, AX132-YCD Cytokeratin 13 14, 46, 87, 187

AM133-5M, AX133-YCD, MU133-UC Cytokeratin, High MW (Basic) 14, 46, 90, 187


c-erbB-2 (HER-2/neu) 13, 24, 45, 81

AM135-5M, AM135-10M, AX135-YCD Breast Cancer Antigen BCA 225 11, 23, 43, 58,

184

AM142-5M, AM142-10M, AX142-YCD, MU142-UC Cytokeratin 8 14, 25, 46, 86,

187

AM143-5M, AX143-YCD, MU143-UC Cytokeratin 18 14, 46, 88, 187


187

AM152-5M, AX152-YCD, MU152-UC CDw75 (B Cell) 13, 45, 80, 186

AM153-5M, AX153-YCD, MU153-UC CD74 (B Cell) 13, 45, 77, 186

AM154-5ME, AX154-YCDE, MU154-UCE HLA-DR 15, 47, 103, 188

AM156-5M, AX156-YCD, MU156-UC CD45RC (T Cell) 12, 45, 74, 186

AM157-5M, AM157-10M, AX157-YCD CD45RA (B Cell) 12, 24, 45, 73,

186

AM158-5M, AM158-10M, AX158-YCD, MU158-UC B Cell 11, 43, 54, 184

AM159-5M, AX159-YCD CD43 & CD45RA Cocktail 12, 44, 72, 186

AM163-5M, AX163-YCD, MU163-UC Vimentin,Non-Hematopoietic 17, 50, 134, 191

AM164-5M, MU164-UC, AX164-YCD Myeloid specific Antigen 16, 48, 114, 189

AM165-5M, AX165-YCD, MU165-UC Macrophage 15, 48, 109, 189

211


[email protected]


AM167-5M, AX167-YCD, MU167-UC Collagen III 13, 48, 83, 187

AM171-5M, AM171-10M, AX171-YCD, MU171-UC Heat Shock Protein 27 (HSP 27) 13, 26, 47, 101,

188


Progesterone Receptor 16, 27, 49, 124

AM181-5M, AX181-YCD, MU181-UC Cytokeratin, Pan 14, 46, 91, 187



14, 25, 47, 94, 187

AM190-5M, AM190-10M, AX190-YCD, MU190-UC pS2 Estrogen Inducible Protein 17, 27, 50, 126,

190

AM195-5M, AM195-10M, AX195-YCD, MU195-UC p53 Protein 16, 26, 49, 120,

190

AM201-5M, AX201-YCD, MU201-UC Cytokeratin 10 14, 46, 87, 187

AM216-5M, AX216-YCD Myeloid specific Antigen 16, 48, 114

AM222-5ME, MU222-UCE Epstein-Barr Virus Early Antigen 47, 95, 188


Placental Alkaline Phosphatase (PLAP)

16, 27, 49, 123, 190


B Lymphocyte Antigen 36 (BLA.36)

11, 23, 43, 54, 184

AM232-5M, AM232-10M, AX232-YCD CD31 12, 24, 44, 70,

185

AM236-5M, AM236-10M, AX236-YCD, MU236-UC CD34 (Endothelial Cell) 12, 24, 44, 71,

185

AM238-5M, AM238-10M, AX238-YCD, MU238A-UC CD20 (B Cell) 12, 24, 44, 185


190


190

AM241-5M, AX241-YCD, MU241-UC CD31 12, 44, 70, 185

AM243-5M, AX243-YCD Dystrophin 14, 46, 92, 187

AM244-5M, AX244-YCD Dystrophin 14, 47, 92, 187


187


Proliferating Cell Nuclear Antigen (PCNA)

16, 27, 49, 125, 190

AM254-5M, MU254-UCE Cytomegalovirus (CMV) 46, 91


187


Androgen Receptor 11, 23, 43, 53, 184

AM258-5M, AX258-YCD, MU258-UC CD3 (T Cell) 12, 44, 63


CD8 (T Cell,Suppressor/Cytotoxic) 12, 44, 65

AM265-5M, AX265-YCD, MU265-UC CD20 (B Cell) 12, 44, 68

AM266-5M, AX266-YCD CD21 (B Cell) 12, 44, 68

AM268-5M, AX268-YCD, MU268-UC CD56 (Natural Killer Cell,NCAM) 13, 45, 75

AM269-5M, AX269-YCD CD71 (Transferrin Receptor) 13, 45, 76

AM270-5M, AX270-YCD CD11b (C3bi Receptor) 12, 44, 66


Estrogen Receptor 14, 25, 47, 95, 188


AM273-5M, AX273-YCD Cytokeratin cocktail, Broad Spectrum 14, 46, 90, 187

AM287-5M, AM287-10M, AX287-YCD Bcl-2 Oncoprotein 11, 23, 43, 55,

184

AM289-5M, AX289-YCD, MU289-UC Heat Shock Protein (HSP-70) 15, 47, 101, 188

AM291-5M, AM291-10M, AX291-YCD Cytokeratin, High MW 14, 25, 46, 90,

187

AM297-5M, AM297-10M, AX297-YCD, MU297-UC Ki-67 Antigen, Proliferating Cell 15, 26, 48, 107,

189

AM298-5M, AX298-YCD, MU298-UC CD29 12, 44, 69, 185


p34cdc2 (Cyclin Dependent Kinase) 16, 49, 119, 190

AM302-5M, AM302-10M, AX302-YCD, MU302-UC CD15 12, 24, 44, 67,

185

AM305-5M, AX305-YCD, MU305-UC CD43 (T Cell, Leukosialin) 12, 44, 72, 186


CD44 (Phagocytic Glycoprotein-1,HCAM) 12, 45, 72, 186

AM314-5M, AX314-YCD, MU314-UC CD57 (Natural Killer Cell) 13, 45, 75, 186

AM315-5M, AM315-10M, MU315-UC, AX315-YCD Cytokeratin 20 14, 25, 46, 89,

187

AM316-5M, AM316-10M, MU316-UC, AX316-YCD Epithelial-Specific Antigen 14, 25, 47, 94,

188


p105 Proliferation-Associated Nuclear Antigen 16, 49, 118,

AM318-5M, AX318-YCD, MU318-UC c-myc Protein 13. 45, 83, 187

AM319-5M, AX319-YCD, MU319-UC c-erbB-3 (HER-3) 13, 24, 45, 81

AM320-5M, AX320-YCD, MU320-UC CD45RB 12, 45, 73, 186

AM322-5M, AM322-10M, AX322-YCD, MU322-UC CD3 (T Cell) 12, 23, 44, 63,

185

AM325-5M, AX325-YCD CD66 13, 45, 76, 186

AM327-5M, AM327-10M, AX327-YCD CD30 (Ki-1 Antigen) 12, 24, 44, 70,

185


Progesterone Receptor (InSite®PR)

16, 27, 49, 125, 190


Myosin Heavy Chains, Smooth Muscle 16, 26, 49, 115


Caldesmon, High MW, Smooth muscle 11, 43, 59

AM333-5M, AX333-YCD, MU333-UC Calponin 11, 43, 59, 184

AM336-5M, AX336-YCD, MU336-UC GLEPP-1 14, 47, 99

AM337-5M, AM337-10M, AX337-YCD, MU337-UC Factor XIII Subunit A 14, 25, 47, 97,

188


CD45 (Leukocyte common Antigen,LCA) 11, 43, 52

AM351-5M, AM351-10M, AX351-YCD, MU351-UC CD30 (Ki-1 Antigen) 12, 24, 44, 70

AM354-5M, AX354-YCD, MU354-UC CD71 (Transferrin Receptor) 13, 45, 77

AM355-5M, AM355-10M, AX355-YCD, MU355-UC CD99 (MIC2 Antigen) 13, 24, 45, 78

AM356-5M, AM356-10M, AX356-YCD, MU356-UC Chromogranin A 13, 24, 45, 82,

187

AM357-5M, AX357-YCD, MU357-UC Cytokeratin, Pan 14, 46, 91

212


www.biogenex.com


AM358-5M, AX358-YCD, MU358-UC Mucin 2 (MUC2) 15, 48, 113

AM359-5M, AX359-YCD bFGF (Basic Fibroblast Growth Factor) 11, 43, 57

AM361-5M, AM361-10M, AX361-YCD, MU361-UC Melan-A (MART-1) 15, 26, 48, 109,

189

AM362-5ME, AX362-YCDE, MU362-UCE

Papillomavirus Type 16 (HPV-16) 16, 49, 121

AM363-5M, AM363-10M, AX363-YCD, MU363-UC Synaptophysin 17, 27, 50, 129,

191

AM365-5M, AM365-10M, AX365-YCD, MU365-UC Carcinoembryonic Antigen (CEA) 13, 23, 43, 61,

185

AM366-5M, AX366-YCD, MU366-UC IgM 15, 48, 104, 188

AM367-5M, AX367-YCD, MU367-UC IgG 15, 48, 104, 188


Estrogen Receptor (InSite® ER) 14, 25, 47, 96, 188

AM369-5M, AM369-10M, AX369-YCD, MU369-UC Kappa Light Chain 15, 26, 48, 106,

189

AM370-5M, AM370-10M, AX370-YCD, MU370-UC Ki-67 Antigen, Proliferating Cell 15, 26, 48, 107,

189


CD45 Cocktail (Leukocyte Antigen, LCA)

12, 24, 45, 73, 186


Cytokeratin cocktail, Broad Spectrum

14, 25, 46, 90, 187


Terminal Deoxynucleotidyl Transferase (TdT) 13, 27, 50, 130

AM374-5M, AX374-YCD, MU374-UC J Chain 15, 48, 106


Platelet-Derived Growth Factor (PDGF) 16, 49, 123


Transforming Growth Factor (TGF),Alpha 17, 50, 133

AM379-5M, AM379-10M, AX379-YCD, MU379-UC Collagen IV 14, 46, 83

AM380-5M, AM380-10M, AX380-YCD Myelin Basic Protein 16, 26, 48, 113

AM381-5M, AX381-YCD, MU381-UC Muscle Actins 16, 48, 113

AM382-5M, AX382-YCD, MU382-UC Aberrant Endothelial Cell 11, 43, 51

AM387-5M, AX387-YCD, MU387-UC Osteonectin 16, 49, 118

AM388-5M, AX388-YCD, MU388-UC Alpha-1-Antichymotrypsin 11, 43, 52

AM390-5M, AM390-10M, AX390-YCD, MU390-UC E-Cadherin 14, 25, 47, 93


Multi-Drug Resistance Marker (P-Glycoprotein) 15, 48, 113

AM392-5M, AM392-10M, AX392-YCD, MU392A-UC CDX-2 13, 24, 45, 81,

186


Human Chorionic Gonadotropin (hCG) Beta 15, 48, 103

AM396-5M, AX396-YCD, MU396-UC p27 (Kip1) 16, 49, 119

AM397-5M, AM397-10M, AX397-YCD, MU397-UC TTF-1 17, 27, 50, 131,

191

AM410-5M, AM410-10M, AX410-YCD, MU410-UC Ki-67 15, 26, 48, 106,

189

AM412-5M, AM412-10M, AX412-YCD, MU412-UC Tau 17, 27, 50, 129,

191


AM414-5M, AM414-10M, AX414-YCD, MU414-UC CD79a 13, 24, 45, 77,

186


186

AM418-5M, AM418-10M, AX418-YCD, MU418-UC P63 16, 27, 49, 121,

190

AM419-5M, AM419-10M, AX419-YCD, MU419-UC Mast Cell Tryptase 26, 48, 109, 189


185


185


186

AM424-5M, AM424-10M, AX424-YCD, MU424-UC CA19-9 11, 23, 43, 58,

184

AM429-5M, AM429-10M, AX429-YCD, MU429-UC CA125 11, 23, 43, 59,

184


185


185

AM432-5M, AM432-10M, AX432-YCD, MU432-UC Myf4 16, 26, 49, 114,

189

AM433-5M, AM433-10M, AX433-YCD, MU433-UC Mesothelin 15, 26, 48, 110,

189

AM434-5M, AX434-YCD, MU434-UC p21/WAF1 16, 49, 119, 190

AM437-5M, AX437-YCD, MU437-UC CD16 12, 44, 67, 185

AM438-5M, AX438-YCD, MU438-UC CD2 11, 44, 62, 185

AM439-5M, AX439-YCD, MU439-UC CD22 12, 44, 69, 185

AM441-5M, AX441-YCD, MU441-UC CD105 13, 45, 79, 186

AM446-5M, AM446-10M, AX446-YCD, MU446-UC Inhibin-Alpha 15, 26, 48, 105,

189


Mouse 4A4+ Mouse34BE12+ Rabbit13H4

16, 27, 147,190


185


185

AM454-5M, AM454-10M, AX454-YCD, MU454-UC MSH6 15, 26, 48, 112

,189

AM455-5M, AM455-10M, AX455-YCD, MU455-UC MUC4 15, 26, 48, 112,

189

AM456-5M, AM456-10M, AX456-YCD, MU456-UC MUC5AC 15, 26, 48, 112,

189

AM457-5M, AM457-10M, AX457-YCD, MU457-UC PAX-5 16, 27, 49, 122,

190

AM459-5M, AM459-10M, AX459-YCD, MU459-UC Tau 17, 27, 50, 129,

191

AM467-5M, AX467-YCD, MU467-UC Cathepsin D 11, 44, 62

AM487-5M, AM487-10M, AX487-YCD, MU487-UC ACTH 11, 23, 43, 51,

184

AM488-5M, AM488-10M, AX488-YCD, MU488-UC Fascin 14, 25, 47, 97,

188

AM490-5M, AM490-10M, AX490-YCD, MU490-UC CD1a 11, 23, 44, 62,

185

AM505-5M, AM505-10M, AX505-YCD, MU505-UC GLUT-1 14, 25, 47, 100,

188

213


[email protected]


AM511-5M, AM511-10M, AX511-YCD, MU511-UC Sarcomeric Actin 17, 27, 50, 128,

190

AM525-5M, AM525-10M, AX525-YCD, MU525-UC Villin 17, 27, 50, 134,

191

AM529-5M, AM529-10M, AX529-YCD, MU529-UC TIA -1 17, 27, 50, 132

AM532-5M, AM532-10M, AX532-YCD, MU532-UC PAP 16, 27, 49, 121,

190

AM534-5M, AM534-10M, AX534-YCD, MU534-UC CD227 (MUCIN 1) 15, 26, 45, 80,

186

AM535-5M, AM535-10M, AX535-YCD, MU535-UC Tyrosinase 17, 27, 50, 133,

191

AM536-5M, AM536-10M, AX536-YCD, MU536-UC Melanoma gp100 15, 26, 48, 110,

189


185

AM539-5M, AM539-10M, MU539-UC Glypican-3 (GPC3) 25, 47, 100, 188

AM540-5M, AM540-10M, MU540-UC p16 (INK4a) 26, 49, 118, 190

AM543-5M, AM543-10M, AX543-YCD, MU543-UC RCC (Renal Cell Carcinoma) 17, 27, 50, 126,

190

AM544-5M, AM544-10M, AX544-YCD, MU544-UC ZAP-70 17, 27, 50, 135,

191


186

AM550-5M, AM550-10M, AX550-YCD, MU550-UC HSA 15, 25, 48, 103,

188

AM554-5M, AM554-10M, AX554-YCD, MU554-UC MiTF 15, 26, 48, 110,

189

AM570-5M, AM570-10M, AX570-YCD, MU570-UC DOG1 14, 25, 46, 92,

187


187

AM583-5M, AM583-10M, AX583-YCD, MU583-UC Calretinin 11, 23, 43, 60,

184

AM587-5M, AM587-10M, AX587-YCD, MU587-UC Cytokeratin 7&8 14, 25, 46, 86,

187

AM6010 TO AM7990 AR6010 TO AR6600 Slide Barcode Labels 29

AM701-5M, AM701-10M, AY701-YCD, MU701-UC Napsin A 16, 26, 49, 116,

189

AM702-5M, AM702-10M, AY702-YCD, MU702-UC CD7 12, 23, 44, 64,

185

AM703-5M, AM703-10M, AY703-YCD, MU703-UC MLH1 15, 26, 48, 111,

189

AM705-5M, AM705-10M, AY705-YCD, MU705-UC Cytokeratin 4 13, 25, 46, 84,

187

AM708-5M, AM708-10M, AY708-YCD, NU708-UC Bcl-6 11, 23, 43, 55

AM736-5ME, AM736-10ME, AY736-YCDE, MU736-UCE

PGP9.5 16, 27, 49, 123

AN449-5ME, AN449-10ME, AX449-YCDE, NU449-UCE

P504S (AMACR) 16, 26, 49, 120, 190

AN462-5M, AN462-10M, AX462-YCD, NU462-UC Bcl-x 11, 23, 43, 55,

184

AN463-5M, AN463-10M, AX463-YCD, NU463-UC PDGF Receptor-ß 16, 27, 49, 122,

190

AN465-5M, AN465-10M, AX465-YCD, NU465-UC CD117 13, 24, 45, 79,

186

AN466-5M, AN466-10M, AX466-YCD, NU466-UC ZAP-70 17, 27, 50, 135,

191


AN471-5ME, AN471-10ME, AX471-YCDE ErbB-2/HER-2 13, 24, 47, 95


EGFR(PAN) 14, 25, 47, 93

AN474-5M, AN474-10M, AX474-YCD, NU474-UC Cyclin D1 13, 24, 46, 84,

187

AN477-5M, AN477-10M, AX477-YCD, NU477-UC CD3 Epsilon 12, 23, 44, 62,

185

AN480-5M, AN480-10M, AX480-YCD, NU480-UC Stat 5-Alpha (C-term) 17, 27, 50,

128,191

AN481-5M, AN481-10M, AX481-YCD, NU481-UC GCDFP-15 14, 25, 47, 98,

188


186

AN484-5M, AN484-10M, AX484-YCD, NU484-UC Cytokeratin 5 13, 25, 46, 85



AN489-5M, AN489-10M, AX489-YCD, NU489-UC Ep-CAM 14, 25, 47, 94,

188

AN492-5M, AN492-10M, AX492-YCD, NU492-UC p27 (Kip1) 16, 26, 49, 119,

190


185

AN500-5M, AN500-10M, AX500-YCD, NU500-UC Cytokeratin 6 13, 25, 46, 85,

187

AN502-5M, AN502-10M, AX502-YCD, NU502-UC Calponin-1 (C-term) 11, 23, 43, 60,

184

AN503-5M, AN503-10M, AX503-YCD, NU503-UC Survivin 17, 27, 50, 129,

191

AN504-5M, AN504-10M, AX504-YCD, NU504-UC MMP-9 15, 26, 48, 111,

189

AN507-5M, AN507-10M, AX507-YCD, NU507-UC Caldesmon 11, 23, 43, 59,

184


Estrogen Receptor (ER Alpha) 14, 25, 47, 96

AN510-5M, AN510-10M, AX510-YCD, NU510-UC Beta-Catenin 11, 23, 43, 56

,184


185

AN513-5M, AN513-10M, AX513-YCD, NU513-UC CD8 (C-Term) 12, 23, 44, 65,

185


185

AN516-5M, AN516-10M, AX516-YCD, NU516-UC Factor XIII A 14, 25, 47, 97,

188


186

AN519-5M, AN519-10M, AX519-YCD, NU519-UC Cyclin E2 13, 25, 46, 84,

187

AN520-5M, AN520-10M, AX520-YCD, NU520-UC TFPU.1 17, 27, 52, 130,

191

AN521-5M, AN521-10M, AX521-YCD, NU521-UC Paxillin (N-Term) 16, 27, 49, 122,

190

AN522-5M, AN522-10M, AX522-YCD, NU522-UC NGF Receptor 16, 26, 49, 117,

190

AN523-5M, AN523-10M, AX523-YCD, NU523-UC Catenin (p120) 16, 26, 44, 61,

185

AN524-5M, AN524-10M, AX524-YCD, NU524-UC PDCD4 16, 27, 49, 122,

190

214


www.biogenex.com


AN527-5M, AN527-10M, AX527-YCD, NU527-UC Ki-67 15, 26, 48, 107,

189


187

AN538-5M, AN538-10M, AX538-YCD, NU538-UC P504S (AMACR) 16, 26, 49, 120,

190

AN541-5M, AN541-10M, AX541-YCD, NU541-UC Bcl-2 11, 23,43, 54,

184

AN546-5M, AN546-10M, AX546-YCD, NU546-UC CD11b/ITAM 12, 24, 44, 66,

185

AN555-5M, AN555-10M, AX555-YCD, NU555-UC CD11c 12, 24, 44, 66,

185


187

AN558-5M, AN558-10M, AX558-YCD, NU558-UC Oct 2 16, 26, 49, 117,

190

AN564-5M, AN564-10M, AX564-YCD, NU564-UC Mucin 1 (MUC1) 15, 26, 48, 112,

189

AN565-5M, AN565-10M, AX565-YCD, NU565-UC N-Cadherin 16, 26, 49, 116,

189

AN567-5M, AN567-10M, AX567-YCD, NU567-UC Apolipoprotein E 11, 23, 43, 53,

184

AN569-5M, AN569-10M, AX569-YCD, NU569-UC Fibronectin 14, 25, 47, 98,

188

AN576-5M, AN576-10M, AX576-YCD, NU576-UC Topo 2 Alpha 17, 27, 50, 132,

191

AN577-5M, AN577-10M, AX577-YCD, NU577-UC PMS2 16, 27, 49, 124,

190

AN584-5M, AN584-10M, AX584-YCD, NU584-UC Wilm's Tumor 17, 27, 50, 135,

191

AN588-5M, AN588-10M, AX588-YCD, NU588-UC Cytokeratin 5 & 6 13, 25, 46, 85,

187

AN710-5ME, AN710-10ME, AY710-YCDE, NU710-UCE

Estrogen Receptor-Alpha 14, 25, 47, 96


Progesterone Receptor 16, 27, 49, 124

AN712-5M, AN712-10M, AY712-YCD, NU712-UC S100P 17, 27, 50, 127,

190

AN713-5M, AN713-10M, AY713-YCD, NU713-UC S100 Beta 17, 27, 50, 127,

190

AN714-5M, AN714-10M, AY714-YCD, NU714-UC PMSA 17, 27, 50, 126,

190

AN715-5M, AN715-10M, AY715-YCD, NU715-UC Lambda Light Chain 15, 26, 48, 108,

189

AN716-5M, AN716-10M, AY716-YCD, NU716-UC CD146 13, 24, 45, 80,

186

AN717-5M, AN717-10M, AY717-YCD, NU717-UC Cytokeratin 4 13, 25, 46, 84,

187

AN718-5M, AN718-10M, AY718-YCD, NU718-UC Claudin-5 13, 24, 45, 82,

187

AN719-5M, AN719-10M, AY719-YCD, NU719-UC CD79a 13, 24, 45, 77,

186


CD63 13, 24, 45, 75, 186


CD48 13, 24, 45, 74


185

AN723-5M, AN723-10M, AY723-YCD, NU723-UC Bcl-2 11, 23, 43, 55,

184


AN724-5M, AN724-10M, AY724-YCD, NU724-UC Oct 4 16, 28, 49, 118,

190

AN725-5M, AN725-10M, AY725-YCD, NU725-UC E-Cadherin 14, 25, 47, 93,

187


c-erbB-2 (HER-2/neu) 13, 24, 45, 81

AN727-5M, AN727-10M, AY727-YCD, NU727-UC Ki-67 14, 25, 48, 95,

188

AN728-5M, AN728-10M, AY728-YCD, NU728-UC p53 16, 26, 49, 120,

190


185


Cytokeratin 5 & Cytokeratin 14 13, 25, 46, 85


BOB1 11, 23, 43, 58


CD41/Integrin Alpha IIb 12, 24, 44, 71


186


AN735-5M, AN735-10M, AY735-YCD, NU735-UC Insulin 15, 26, 48, 106,

189


CD205 13, 24, 45, 80

AN738-5M, AN738-10M, AY738-YCD, NU738-UC NGF Receptor 16, 26, 49, 117,

190


CD103 13,24, 45, 79

AN740-5M, AN740-10M, AY740-YCD, NU740-UC CD8 12, 23, 44, 65

AN741-5M, AN741-10M, AY741-YCD, NU741-YCD CD35 12, 24, 44, 71

AN743-5M, AN743-10M, AY743-YCD, NU743-UC MSH2 15, 26, 48, 111

AR009-5R, AW009-YCD Carcinoembryonic Antigen (CEA) 11, 43, 61, 185

AR012-5R,AW012-YCD, PU012-UP Myoglobin 16, 49, 115, 189

AR015-5R, AW015-YCD, PU015-UP Alpha-1-Antitrypsin 11, 43, 52, 184

AR019-5R, AW019-YCD, PU019-UP Gastrin 14, 47, 98, 188

AR020-5R, AR020-10R, AW020-YCD, PU020-UP

Glial Fibrillary Acidic Protein (GFAP) 14, 47, 99, 188

AR021-5R, AW021-YCD Hemoglobin A 15, 47, 102, 188

AR024-5R, AR024-10R, AW024-YCD, PU024-UP Lysozyme 15, 26, 48, 108,

189

AR026-5R, AW026-YCD Follicle Stimulating Hormone (FSH) 14, 47

AR033-5R, AW033-YCD Thyroid Stimulating Hormone (TSH) 17, 50, 131

AR038-5R, AW038-YCD Estradiol 14, 47, 95, 188

AR039-5R, AW039-YCD, PU039-UP Glucagon 14, 47, 99, 188

AR040-5R, AR040-10R, AW040-YCD, PU040-UP Placental Lactogen (hPL) 16, 27, 49, 123,

190

AR045-5R, AW045-YCD, PU045-UP IgA 15, 48, 103

215


[email protected]


AR049-5R,AW049-YCD, PU049-UP Lambda Light Chain 15, 48, 108, 189

AR050-5R, AW050-YCD, PU050-UP IgG 15, 48, 104, 188

AR058-5R, AR058-10R, AW058-YCD, PU058-UP S-100 Protein 17, 27, 50, 127,

190

AR067-5R, AW067-YCD Secretin 17, 50, 128, 191

AR069-5R, AW069-YCD, PU069-UP Substance P 17, 50, 128, 191

AR078-5R,AR078-10R, AW078-YCD, PU078-UP Laminin 15, 26, 48, 108,

189

AR082-5RE, PU082-UPE Hepatitis B Virus Core Antigen (HBcAg) 47, 102

AR084-5RE, AW084-YCDE, PU084-UPE

Herpes Simplex Virus Type I (HSV I) 15, 47, 102


Herpes Simplex Virus Type II (HSV II) 15, 47, 102

AR125-5RE, PU125-UPE Toxoplasma gondii 50, 132

AR249-5R, AW249-YCD, PU249-UP

Glutathione S-Transferase Pi (GST Pi) 14, 47, 100, 188

AR335-5RE, AW335-YCDE, PU335-UPE EGFR 14, 47, 93

AR345-5R, AW345-YCD, PU345-UP BRCA1 Protein 11, 43, 55

AR347-5R, AW347-YCD, PU347-UP Bax Protein 11, 43, 54

AR373-5R,AW373-YCD Terminal Deoxynucleotidyl Transferase (TdT) 17, 50, 130, 191


Platelet-Derived Growth Factor (PDGF) 16, 49, 124

AR385-5R, AR385-10R, AW385-YCD, PU385-UP Estrogen Receptor-Beta (ER-β) 14, 25, 47, 96

AR413-5R, AR413-10R, AW413-YCD, PU413-UP Calretinin 11, 23, 60, 184

AR427-5R,AR427-10R, AW427-YCD, PU427-UP IgM 15, 48, 105, 188

AR440-5R, AR440-5RSG, PU440-UP, AW440-YCD lgD 15, 48, 104, 188

AR442-5RE,AR442-10RE, AW442-YCDE, PU442-UPE

Helicobacter pylori 15, 26, 47, 101

AR447-5R, AR447-10R, AW447-YCD, PU447-UP Cyclin D1 13, 24, 46, 83,

187

AR460-5R,AR460-10R, AW460-YCD, PU460-UP CD23 12, 24, 44, 69,

185

AR483-5R, AR483-10R, AW483-YCD, PU483-UP VEGF 17, 27, 50, 134,

191

AR496-5R, AR496-10R, AW496-YCD, PU496-UP Myeloperoxidase (MPO) 16, 26, 49, 114,

189

AR530-5R,AR530-10R, AW530-YCD, PU530-UP VIP 17, 27, 50, 135,

191

AR704-5R, AR704-10R, AW704-YCD, PU704-UP Claudin 1 13, 24, 45, 82,

187

AR706-5R, AR706-10R, AW706-YCD, PU706-UP Catenin Delta 1 (p120) 11, 23, 44, 61,

185

AR707-5R, AR707-10R, AW707-YCD, PU707-UP HGH 15, 26, 47, 101,

188

AS1000 Xmatrx Nano 4, 7, 11

AS1010 Xmatrx Mini 5, 7, 11

AS4000RX Xmatrx Infinity 6, 11

AS4040B Xmatrx Elite 2, 7, 11

AS6030 i6000 Diagnostic 8, 23

AS6040 i6000 Infinity 9, 23


BBLS500 i500 S Bar Code Labeling

System 10, 30

BS-1310-25 Power Block Reagent, Ready-to-use 177

DDF400-50KE

Super Sensitive One Step Polymer-HRP ISH Detection System

158

DF400-YADE XISH One Step Polymer-HRP ISH Detection System 19, 158

DF500-20XE eFISH Histo Kit 163

DF510-20XE eFISH Cyto Kit 163

DF530-40KE eFISH U Kit 163

EEK000-10K, EK000-10X Pepsin 4-Pack Kit 19, 30

EK000-5K Pepsin 3-Pack Kit 38

EK001-10K, EK001-10X Trypsin 4-Pack Kit 19, 30

EK001-5K Trypsin 3-Pack Kit 38

EK002-10K, EK002-10X Protease XXIV 4-Pack Kit 19, 30

EK002-5K Protease XXIV 3-Pack Kit 38

EK004-5K Diastase (a-Amylase) 30, 38

FFB-001M to FB-743N Positive Control on Barrier

Slides 184 - 191

FG-001M to FG-743N Positive Control Slides 184 - 191

FP038-10XE, FP038-20XE

eFISH MDM2/CEN 12 Dual Color Probe 162

FP039-10XE, FP039-20XE

eFISH CEN 17/HER2 Dual Color Probe 162

FP040-10XE, FP040-20X E

eFISH EGFR/CEN 7 Dual Color Probe 162

FP041-10XE , FP041-20XE

eFISH p16/CEN 9 Dual Color Probe 162

FP042-10XE, FP042-20XE

eFISH FGFR1/CEN 8 Dual Color Probe 162

FP043-10XE, FP043-20XE

eFISH NMYC/2q11 Dual Color Probe 162

FP044-10XE, FP044-20XE

eFISH 1p36/1q25 Dual Color Probe 162

FP045-10XE, FP045-20XE

eFISH 19q13/19p13 Dual Color Probe 162

FP046-10XE, FP046-20XE

eFISH VHL/CEN 3 Dual Color Probe 162

FP047-10XE, FP047-20XE

eFISH MET/CEN 7 Dual Color Probe 162

FP048-10XE, FP048-20XE

eFISH EWSR1 Dual Color Break Apart Probe 162

FP049-10XE, FP049-20XE

eFISH SYT Dual Color Break Apart Probe 162

FP050-10X, FP050-20XE eFISH CHOP Dual Color Break Apart Probe 162

FP051-10XE , FP051-20XE

eFISH TFE3 Dual Color Break Apart Probe 162

FP052-10XE, FP052-20XE

eFISH COL1A1/PDGFB Dual Color Dual Fusion Probe 162

FP053-10XE , FP053-20XE

eFISH PDGFB Dual Color Break Apart Probe 162

216


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FP054-10XE, FP054-20XE

eFISH COL1A1 Dual Color Break Apart Probe 162

FP055-10XE, FP055-20XE

eFISH FGFR2/CEN 10 Dual Color Probe 162

FP056-10XE, FP056-20XE

eFISH ALK Dual Color Break Apart Probe 162

FP057-10XE, FP057-20XE

eFISH CCND1/IGH Dual Color Dual Fusion Probe 162

FP058-10XE, FP058-20XE

eFISH FUS Dual Color Break Apart Probe 162

FP059-10XE, FP059-20XE

eFISH PIK3CA/CEN 3 Dual Color Probe 162

FP060-10XE, FP060-20XE

eFISH ROS1 Dual Color Break Apart Probe 162

FP061-10XE, FP061-20XE

eFISH RET Dual Color Break Apart Probe 162

FP062-10XE, FP062-20XE

eFISH TP53/CEN 17 Dual Color Probe 162

FP063-10XE, FP063-20XE

eFISH CCND1/CEN 11 Dual Color Probe 162

FP064-10XE, FP064-20XE

eFISH CMYC Dual Color Break Apart Probe 162

FP065-10XE, FP065-20XE

eFISH CMYC/CEN 8 Dual Color Probe 162

FP066-10XE, FP066-20XE

eFISH TERT/5q31 Dual Color Probe 162

FP067-10XE, FP067-20XE

eFISH CMYC/IGH Dual Color Dual Fusion Probe 162

FP068-10XE, FP068-20XE

eFISH EGR1/5p15 Dual Color Probe 162

FP069-10XE , FP069-20XE

eFISH CCND1 Dual Color Break Apart Probe 162

FP070-10XE , FP070-20XE

eFISH IGH Dual Color Break Apart Probe 162

FP071-10XE , FP071-20XE

eFISH BCR/ABL Dual Color Dual Fusion Probe 162

FP072-10XE, FP072-20XE

eFISH AML1/ETO Dual Color Dual Fusion Probe 162

FP073-10XE , FP073-20XE

eFISH PML/RARA Dual Color Dual Fusion Probe 162

FP074-10XE, FP074-20XE eFISH BCL2/IGH Dual Color Dual Fusion Probe 162

FP075-10XE, FP075-20XE

eFISH BIRC3/MALT1 Dual Color Dual Fusion Probe 162

FP076-10XE, FP076-20XE eFISH ETV6/RUNX1 Dual Color Dual Fusion Probe 162

HHK057-5KE Antigen Retrieval AR-10 (10X) 37

HK058-5K, HK058-20K Antigen Retrieval AR-10 RTU 37

HK079-5K, HK079-7K Super Mount 180

HK080-5K, HK080-9K Antigen Retrieval Citra Plus (10X) 36

HK081-5KE, HK081-20KE Antigen Retrieval Citra Plus RTU 36

HK085-5KE Power Block (10X) 177

HK086-5K, HK086-9K Antigen Retrieval Citra (10X) 36

HK087-5KE, HK087-20KE Antigen Retrieval Citra RTU 36

HK091-5KE Phosphate Buffer Saline (PBS) 176

HK092-5K AEC Substrate Pack Kit 178

HK098-5K Tris Buffer, Conc pH 7.6 176


HK099-5K Aqueous Mounting Medium 180

HK100-5K, HK100-9K Hematoxylin 180

HK102-5K, HK102-10K, HK102-20X Avidin Biotin Blocking Kit 28, 177

HK111-5K, HK111-50K Peroxide Block 177

HK112-5K, HK112-9KE Blocking Reagent II Goat Serum 177

HK114-5K Blocking Reagent II Rabbit Serum 177

HK119-7M SS Mouse Negative Control 179

HK124-7K, HK124-9K Liquid DAB Chromogen 178

HK129-5KE AEC Substrate Kit, Conc 178

HK139-06K AEC Chromogen 178

HK139-50KE One Step AEC Solution 178

HK144-5KE Elegance Red Substrate Pack 179

HK156-5KE Common Ab Diluent 176

HK157-5KE S-A HRP Diluent 176

HK165-5KE Link Diluent 176

HK169-5K H & E Retrieval Solution (10X) 37

HK182-5KE Fast Red Substrate Pack 179

HK183-5KE New Fuchsin Substrate Pack 179

HK268-UK Conc Multilink 142

HK320-UK Conc Strept Label HRP 142

HK321-UK Conc Strept Label AP 142

HK325-UM Conc Mouse Link 142

HK326-UR Conc Rabbit Link 142

HK327-UG Conc Goat Link 142

HK330-5K, HK330-9K Super Sensitive HRP Label 142

HK331-5K, HK331-9K SS Strept Alka Phos Label 142

HK335-5M, HK335-9M SS Mouse Link 142

HK336-5R, HK336-9R SS Rabbit Link 142

HK337-5G SS Goat Link 142

HK338-5T SS Rat Link 142

HK340-5K, HK340-9K SS Multilink 142

HK406-5G Goat, SS Neg Control 179

HK407-5T Rat, SS Neg Control 179

HK408-5R, HK408-7R Rabbit, SS Neg Control 179

HK521-XAK , HK521-XIK EZ-AR1, RTU 30, 35

HK522-XAK , HK522-XIK EZ-AR2, RTU 30, 35

HK542-XAK DAB 2-Component Pack 178

HK543-YOK EZ-AR3 30, 35

HK544-YOK EZ-AR4 30, 35

HK545-XOK EZ-AR Common 30, 35

HK546-XAK EZ-AR1 Elegance, RTU 34

HK547-XAK EZ-AR2 Elegance, RTU 34

HK583-5KE Super Sensitive Wash Buffer, 20X 30, 176

HK584-5KE EZ-DeWax Solution, Conc 29, 32

HK941-YAKE Enhanced Antibody Diluent 176

HK972-YRXE AutoFISH Reagent A 163

HK973-YRXE AutoFISH Reagent B 163

HK981-YRXE AutoFISH Protease 1 Buffer 163

HK982-YRXE AutoFISH Protease 2 Buffer 163

217


[email protected]


HK983-YRXE AutoFISH Pretreatment Reagent 163

HM001-100E Hsa-miR-1 Probe 152, 157

HM007A-100E Hsa-miR-7a Probe 156, 157

HM010B-100E Hsa-miR-10b Probe 152, 157

HM017-100E Hsa-miR-17 Probe 152, 157

HM021-100E Hsa-miR-21 Probe 153, 157



HM126-100E Hsa-miR-126 Probe 153, 157

HM1285-100E Hsa-miR-1285 Probe 156, 157

HM141-100E Hsa-miR-141 Probe 153, 157

HM144-100E Hsa-miR-144 Probe 153, 157



HM150-100E Hsa-miR-150 Probe 154, 157

HM151-100E Hsa-miR-151a-3p Probe 154, 157

HM152-100E Hsa-miR-152 Probe 154, 157

HM155-100E Hsa-miR-155 Probe 154, 157




HM204-100E Hsa-miR-204 Probe 155, 157

HM205-100E Hsa-miR-205 Probe 155, 157


HM222-100E Hsa-miR-222 Probe 155, 157

HM328-100E Hsa-miR-328 Probe 156, 157

HM375-100E Hsa-miR-375 Probe 156, 157

HX016-XAK, HX016-XEK X-DeWax 21

HX017-YIK X-Wash ISH Wash Buffer 21

HX020-YIK X-Wash Buffer, 20X, for Xmatrx 21

HX031-YCDE, HX031-YCX EZ-AR™ 1 for Xmatrx 19




HX035-04X, HX035-10X, HX035-40D Xmount 21

LLA000-ULE Super Sensitive Link Label IHC

Detection System, AP 141

LP000-ULE Super Sensitive Link Label IHC Detection System, HRP 141

MMU121-UC Alpha-Tubulin 43, 53, 184

MU122-UC Beta-Tubulin 43, 56, 184

MU176-UC Beta-Tubulin II 43, 56, 184

MU177-UC Beta-Tubulin III 43, 56, 184

MU178-UC Beta-Tubulin IV 43, 57, 184

MU210-UC Granulocyte 47, 100, 188

MU213-UC Mitochondrial Antigen 48, 111

MU230-UC Nuclear Ribonucleoprotein 49, 117, 190

MU303-UC Blood Group Antigen Lewis A 43, 57, 184

MU304-UC Blood Group Antigen Lewis B 43, 57, 184


MW001-HB Microwave AR Accessory Kit 34

MW001-SU Microwave AR Single Access Kit 34

NNW001-PC Nordic Ware Pressure Cooker 39

PPR026-100E, PR026-YADE ALU Probe II 19, 150, 151

PR031-100E U6 Probe 156, 157

PR032-100E Scramble Probe 156, 157

PR1055-100E, PR1055-YADE Beta Actin 19, 150, 151

PR205-100E, PR205-YADE EBER Probe 19, 150, 151

PR214-100E, PR214-YADE Kappa Probe 19, 151

PR215-100E, PR215-YADE Lambda Probe 19, 151

PR216-100E, PR216-YADE Cyclin D1 Probe 19, 150, 151

PR217-100E, PR217-YADE Oligo d(T) Probe 19, 151

PR225-100E, PR225-YADE Retinoblastoma (RB) Probe 19, 151

PR250-100E, PR250-YADE HPV 16/18 Probe 19, 150, 151

PR251-100E, PR251-YADE HPV 14 Probe 19, 150, 151

QQA900-9L Super Sensitive Link Label IHC

Detection System, Alk Phos 141

QD400-60KE Super Sensitive Non-Biotin HRP, DAB Kit 139

QD410-YAXE Super Sensitive Non-Biotin HRP, DAB Automated Kit 28

QD420-YIKE Super Sensitive Link Label IHC Detection System, HRP/DAB 139

QD430-XAKESuper Sensitive Link Label IHC Detection System, HRP/DAB Mega

139

QD440-XAK Super Sensitive Polymer-HRP IHC Kit 139

QD470-60K, QD470-YCX SS Multilink Kit,HRP/DAB 28, 141

QD550-YCDE, QD550-YCX XVIZ Detection Kit 18

QD610-YADE SS One Step Polymer-HRP IHC Detection System for Xmatrx 28

QD610-YAXE One-Step Polymer-HRP 28

QD620-YIKE One-Step Polymer-HRP 139

QD630-XAKE One-Step Polymer-HRP 139

QP000-5L, QP900-9L Super Sensitive Link HRP IHC Detection System 141

QP300-XAK Mega Multilink/HRP Kit 141

QS200-60K SS Double Stain Polymer Detection Kit I/DAB & Fast Red 144

QS200-YADE XVIZ Doublestaining Detection Kit I 18

QS210-YIK SS Double Stain Polymer Detection Kit I/Large Volume 144

QS400-60K SS Double Stain Polymer Detection Kit II/DAB & Fast Red 144

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QS400-YADE XVIZ Doublestaining Detection Kit II 18

QS410-YIK SS Double Stain Polymer Detection Kit II/Large Volume 144

SSS025-50X Acid Fast Blue Stain Kit 168, 173

SS026-50X, SS026-YAK Alcian Blue PAS Stain 168, 173, 174

SS027-50X, SS027-YAK Alcian Blue Stain 168, 173, 174

SS028-50X, SS028-YAK Congo Red Solution 168, 173, 174

SS029-50X Elastic Stain 169, 173

SS030-50X, SS030-YAK Iron Stain Kit 170, 173, 174

SS032-50X, SS032-YAK PAS Staining Kit 171, 173, 174

SS033-50X, SS033-YAK Gomoris Trichome Blue 169, 173, 174

SS034-50X, SS034-YAK Gomoris Trichome Greeen 170, 173, 174

SS035-50X, SS035-YAK Masson's Trichrome Kit 170, 173, 174

SS036-50X, SS036-YAK Mucicarmine Stain 171, 173, 174

SS037-50X, SS037-YAK Gram Stain Kit 170, 173, 174

SS038-50X, SS038-YAK Azure A satin 169, 173, 174

SS039-50X, SS039-YAK Perodic Acid Schiff (PAS) Diastase Stain 171, 173, 174

SS040-50X, SS040-YAK Safranin O Staining Protocol 172, 173, 174

SS041-50X, SS041-YAK Sudan Black B Stain 172, 173, 174

SS042-50X, SS042-YAK GMS for Fungi Stain Kit 170, 173, 174

SS043-50X, SS043-YAK Oil Red O Stain 169, 173, 174

SS044-50X, SS044-YAK Van Gieson Stain 172, 173, 174

SS045-50K von Kossa stain 173, 174

SS046-50X, SS046-YAK Reticulin/No Counterstain Stain Kit 172, 173, 174

SS047-50X, SS047-YAK Reticulin/No Counterstain Stain Kit 172, 173, 174

SS049-50X, SS049-YAK Geimsa Stain 169, 173, 174

SS050-50X, SS050-YAK Renal Masson's Trichome 171, 173, 174

SS052-50K Alizarin Red Stain 168, 174

SS053-50X, SS053-YAK PAS for Fungi Stain 171, 173, 174

SS054-50X, SS054-YAK Colloidal Iron stain 169, 173, 174

SS055-50X, SS055-YAK Bielschowsky's Silver Stain 169, 173, 174

SS057-50X, SS057-YAK Toluidine blue stain 172, 173, 174

SS058-50X, SS058-YAK Jones' Basement Membrane Stain 170, 173, 174

SS059-50X AFB Green 168, 173

XXT001-PP PAP Pen 29, 181

XT002-CL, XT002-SL OptiPlus Microscope Slides 181

XT013-CL, XT013-SL OptiPlus Barrier Slides, 2/3 Test Area 29, 181

XT014-CL OptiPlus Barrier Slides, 3 X 1/3 Test Area,20 Boxes 29, 181

XT014-SL OptiPlus Barrier Slides, 3 X 1/3 Test Area,72 Slides 29, 181

XT022-CP Cap for Optimiser Vials (24) 28

XT026-601 to XT026-799, XT026-601P to XT026-660P

Optimiser Reagent Vial (20mL) 28

XT026-PR601 to XT026-PR615

Reagent Empty Vial Labeled for User Probe 28


XT026-V24 Unlabeled White Optimiser Vials with Caps and Holders, 20mL 28

XT027-H24 Optimiser Vial Holders (24) 28

XT034-XEX Resin Ribbon i500 PLUS 30

XT035-XBX Label Roll i500 PLUS 30

XT077-AX0601 to XT077-AX0800

User Defined Empty RFID Tagged Vials Two Step IHC 21

XT077-AX0601 to XT0821-AX0999

User Defined Empty RFID Tagged Vials One Step IHC 21

XT077-V24 Empty Reagent Vials for Xmatrx Rx-White 21

XT078-V24 Empty Reagent Vials for Xmatrx Rx-Brown 21

XT079-PR0050 to XT079-PR0099

Empty RFID Vial User Defined for ISH 20

XT101-24X Optimiser Reagent Vials, 20mL 28

XT104-05X i6000 Pipette Tips (960 tips) 20, 22, 29

XT105-01X i6000 Pipette Tips (192 tips) 20, 22, 29

XT108-CL, XT108-SL Barrier Slides (25x25mm) 20, 22

XT114-CL, XT114-SL Barrier Slides, 2 Zones, for Xmatrx 20, 22

XT118-50X, XT118-YRK Coverslips, 25X40mm, for Xmatrx 20, 22

XT121-XBK, XT121-YBX Coverslips, 18X18mm, for Xmatrx 20, 22

XT122-90X, XT122-YQK Coverslips, 25X25mm, for Xmatrx 20, 22

XT126-24V Xmatrx Reagent Vials 20, 22

XT128-CL, XT128-SL Barrier Slides, 18X18mm, for Xmatrx 20, 22

XT132-YCD Xflex Consumable Kit, for Xmatrx 20

XT134-CL, XT134-SL Barrier Slides, 25X40mm, for Xmatrx 20, 22, 29, 181

XT144-YAD ISH Consumable Kit, for Xmatrx 20

XT145-05X Pipette Tips, 200ul (960 tips)/5 Boxes, for Xmatrx 21, 22

XT146-01X Pipette Tips, 200ul (192 tips), for Xmatrx 21, 22

XT147-YAD FISH Consumable Kit, for Xmatrx 20, 22

XT148-YCD Xflex Ultra Consumable Kit, for Xmatrx 20

XT149-V24 Reagent Vial Insert for RFID 21, 22

XT150-YCDE Auto DAB kit for Xmatrx Elite 18

219

Antibody Clone Index

[email protected]

Clone Antibody Cat. No. Page

1.1 DOG1


14, 25, 46, 92, 187

36 E-Cadherin


14, 25, 47, 93

1801 p53 Protein


16, 27, 49, 121, 190

5404Thyroid Stimulating Hormone (TSH)


17, 27, 50, 131

1108-1 Epstein-Barr Virus Early Antigen

AM222-5ME, MU222-UCE 47, 95, 188

113-1 Mitochondrial Antigen MU213-UC 48, 111

4A4 p63 Protein


16, 27, 49, 121, 190

11E 3 CD79a


13, 24, 45, 77, 186

13H4 P504S (AMACR)


16, 26, 49, 120, 190

15E2E2 S-100 Protein


17, 27, 50, 127, 190

1A4 Actin, Smooth Muscle


11, 23, 43, 51, 184

1A5 CD8


12, 23, 44, 64, 185

1A6 Progesterone Receptor


16, 27, 49, 124

1D5 Estrogen Receptor


14, 25, 47, 95, 188

1G8 MUC4


15, 26, 48, 112, 189

2-25LE Blood Group Antigen Lewis B MU304-UC 43, 57, 184

2B3p105 Proliferation-Associated Nuclear Antigen


16, 49, 118

2D4B5 MSH6


15, 26, 48, 112,189

2E7 Calretinin


11, 23, 43, 60, 184


2G9A10F5 TIA -1


17, 27, 50, 132

2H11 Thyroglobulin


17, 27, 50, 130, 191

2H7 CD16 AM437-5M, AX437-YCD, MU437-UC

12, 44, 67, 185

34βE12 Cytokeratin, High MW

AM291-5M, AM291-10M, AX291-YCD

14, 25, 46, 90, 187

34βE12/C51/AE1Cytokeratin cocktail, Broad Spectrum

AM273-5M, AX273-YCD 14, 46, 90, 187

3D9 PGP9.5

AM736-5ME, AM736-10ME, AY736-YCDE, MU736-UCE

16, 27, 49, 123

45M1 MUC5AC


15, 26, 48, 112, 189

4A11 Aberrant Endothelial Cell


11, 43, 51

4B12 CD4


12, 23, 44, 63, 185

4C7 CD5


12, 23, 44, 64, 185

4C9 CD25


12, 24, 44, 69, 185

4D10 p21/WAF1AM434-5M, AX434-YCD, MU434-UC

16, 49, 119, 190

4G11 CD105AM441-5M, AX441-YCD, MU441-UC

13, 45, 79, 186

56C6 CD10


12, 23, 44, 65, 185

58-15 Nuclear Ribonucleoprotein MU230-UC 49, 117, 190

5B2 Mesothelin


15, 26, 48, 110, 189

5C11 GLEPP-1AM336-5M, AX336-YCD, MU336-UC

14, 47, 99

5D3 Cytokeratin 8 & 18


14, 25, 46, 87, 187

6B10 Cytokeratin 4

AM705-5M, AM705-10M, AY705-YCD, MU705-UC

13, 25, 46, 84, 187

7LE Blood Group Antigen Lewis A MU303-UC 43, 57, 184

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8G7G3/1 TTF-1


17, 27, 50, 131, 191

9E10 c-myc ProteinAM318-5M, AX318-YCD, MU318-UC

13. 45, 83, 187

9G11 CD31AM241-5M, AX241-YCD, MU241-UC

12, 44, 70, 185

A103 Melan-A (MART-1)


15, 26, 48, 109, 189

a1A88 Alpha-1-Antichymotrypsin


11, 43, 52

A27-42B Lymphocyte Antigen 36 (BLA.36)


11, 23, 43, 54, 184

A40010 PAP


16, 27, 49, 121, 190

A62020069P AdenovirusAM059-5ME, AX059-YCDE, MU059-UCE

11, 43, 52

AA1 Mast Cell Tryptase


26, 48, 109, 189

AB75 CD2AM438-5M, AX438-YCD, MU438-UC

11, 44, 62, 185

Actin 88 Cocktail Muscle ActinsAM381-5M, AX381-YCD, MU381-UC

16, 48, 113

AE1 Cytokeratin, Low MW


14, 25, 46, 91, 187

AE1 & AE3 Cytokeratin Cocktail


14, 25, 46, 89, 187

AE3 Cytokeratin, High MW (Basic)


14, 46, 90, 187

AE8 Cytokeratin 13 AM132-5M, AX132-YCD 14, 46, 87, 187

AH26 ACTH


11, 23, 43, 51, 184

B01-94-11M-P Carcinoembryonic Antigen (CEA)


11, 23, 43, 60, 185

B01-94-21M-NAProstate Specific Acid Phosphatase (PSAP)


16, 27, 50, 125, 190

B1 CD20 (B Cell)AM265-5M, AX265-YCD, MU265-UC

12, 44, 68

B2 CD21 (B Cell) AM266-5M, AX266-YCD 12, 44, 68


B6.2Tumor-Associated Glycoprotein (TAG-90 BCA)


17, 50, 133, 191

B72.3Tumor-Associated Glycoprotein (TAG-72)


17, 27, 50, 133, 191

Bcl-2/100 Bcl-2 OncoproteinAM287-5M, AM287-10M, AX287-YCD

11, 23, 43, 55, 184

Ber-H2 CD30 (Ki-1 Antigen)

AM327-5M, AM327-10M, AX327-YCD

12, 24, 44, 70, 185

bFGF88bFGF (Basic Fibroblast Growth Factor)

AM359-5M, AX359-YCD 11, 43, 57

BM-1 Myeloid specific Antigen

AM164-5M, MU164-UC, AX164-YCD

16, 48, 114, 189

BM-2 Granulocyte MU210-UC 47, 100, 188

BM204 Cytomegalovirus (CMV)

AM254-5M, MU254-UCE 46, 91

BM-3 Myeloid specific Antigen

AM216-5M, AX216-YCD 16, 48, 114

BP53-12-1 p53 Protein


16, 26, 49, 120, 190

BRA4F1 CD15


12, 24, 44, 67, 185

BRM-22 Heat Shock Protein (HSP-70)


15, 47, 101, 188

BY114 CD66 AM325-5M, AX325-YCD 13, 45, 76, 186

C11 Cytokeratin, PanAM357-5M, AX357-YCD, MU357-UC

14, 46, 91

C15 Cathepsin DAM467-5M, AX467-YCD, MU467-UC

11, 44, 62

C241:5:1:4 CA19-9


11, 23, 43, 58, 184

C3 Alpha-Fetoprotein (AFP)

AM008-5M, AM008-10M, AX008-YCD, MU008A-UC

11, 23, 43, 53, 184

C51 Cytokeratin 8


14, 25, 46, 86, 187

CALP CalponinAM333-5M, AX333-YCD, MU333-UC

11, 43, 59, 184

Cam Vir-1 Papillomavirus Type 16 (HPV-16)

AM362-5ME, AX362-YCDE, MU362-UCE

16, 49, 121

CAN-R9(IHC)-56-2 Wilm's Tumor

AN584-5M, AN584-10M, AX584-YCD, NU584-UC

17, 27, 50, 135, 191

CB11 c-erbB-2 (HER-2/neu)


13, 24, 45, 81

221


[email protected]


CCP58 Mucin 2 (MUC2)AM358-5M, AX358-YCD, MU358-UC

15, 48, 113

CD20/C23 CD20


12, 24, 44, 67, 185

CD68/G2 CD68


13, 24, 45, 76, 186

CDX2-88 CDX-2


13, 24, 45, 81, 186

CEA88 Carcinoembryonic Antigen (CEA)


13, 23, 43, 61, 185

COL-94 Collagen IV


14, 46, 83

CU18 Breast Cancer Antigen BCA 225

AM135-5M, AM135-10M, AX135-YCD

11, 23, 43, 58, 184

D33 Desmin


14, 28, 46, 92,187

D5 ThyroxineAM034A-5M, AX034-YCD, MU034A-UC

17, 50, 131, 191

DC-10 Cytokeratin 18 AM143-5M, AX143-YCD, MU143-UC

14, 46, 88, 187

DCS72 p27 (Kip1)AM396-5M, AX396-YCD, MU396-UC

16, 49, 119

DEK-10 Cytokeratin 10AM201-5M, AX201-YCD, MU201-UC

14, 46, 87, 187

DF1485CD44 (Phagocytic Glycoprotein-1,HCAM)


12, 45, 72, 186

DFT-1 CD43 (T Cell, Leukosialin)


12, 44, 72, 186

DM-1A Alpha-Tubulin MU121-UC 43, 53, 184

DM-1B Beta-Tubulin MU122-UC 43, 56, 184

DO7 p53 Protein


16, 27, 49, 121, 190

Dys1 (Dy4/6D3) Dystrophin AM243-5M, AX243-YCD 14, 46, 92, 187

Dys2 (Dy8/6C5) Dystrophin AM244-5M, AX244-YCD 14, 47, 92, 187

E144 Ep-CAM


14, 25, 47, 94, 188

E17 Bcl-2


11, 23,43, 54, 184

E18 Bcl-x


11, 23, 43, 55, 184


E247 Beta-Catenin


11, 23, 43, 56 ,184

E27 Cytokeratin 17


14, 25, 46, 88, 187

E289 Stat 5-Alpha (C-term)


17, 27, 50, 128,191

E29Epithelial Membrane Antigen (EMA)


14, 25, 47, 94, 187

E89 Caldesmon


11, 23, 43, 59, 184

E980.1 Factor XIII Subunit A


14, 25, 47, 97, 188

EP1 Estrogen Receptor-Alpha


14, 25, 47, 96

EP1039Y NGF Receptor


16, 26, 49, 117, 190

EP1045Y ErbB-2/HER-2AN471-5ME, AN471-10ME, AX471-YCDE

13, 24, 47, 95

EP1102Y Topo 2 Alpha


17, 27, 50, 132, 191

EP1150Y CD8 (C-Term)


12, 23, 44, 65, 185

EP125 Insulin

AN735-5M, AN735-10M, AY735-YCD, NU735-UC

15, 26, 48, 106, 189

EP1255Y MMP-9


15, 26, 48, 111, 189

EP1347Y CD11c


12, 24, 44, 66, 185

EP1373Y Apolipoprotein E


11, 23, 43, 53, 184

EP143 Oct 4


16, 28, 49, 118, 190

EP148 CD48


13, 24, 45, 74

EP1582Y GCDFP-15


14, 25, 47, 98, 188

222


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EP1615Y Cytokeratin 16


14, 25, 46, 88

EP169 CD19


12, 24, 44, 67, 185

EP172 Lambda Light Chain


15, 26, 48, 108, 189

EP176 CD205


13, 24, 45, 80

EP178 CD41/Integrin Alpha IIb


12, 24, 44, 71

EP179 CD53


13, 24, 45, 74,

EP186 S100P


17, 27, 50, 127, 190

EP192 PMSA


17, 27, 50, 126, 190

EP2 Progesterone Receptor


16, 27, 49, 124

EP204 CD4


12, 23, 44, 63, 185

EP206 CD103


13, 24, 45, 79

EP211 CD63


13, 24, 45, 75, 186

EP224 Claudin-5


13, 24, 45, 82, 187

EP24 + EP61 Cytokeratin 5 & Cytokeratin 14


13, 25, 46, 85

EP2417Y CD61


13, 24, 45, 75, 186

EP2880Y Survivin


17, 27, 50, 129, 191

EP2952 CD5


12, 23, 44, 64, 185


EP3 c-erbB-2 (HER-2/neu)


13, 24, 45, 81

EP3093 CD21


12, 24, 44, 68, 185

EP31 NGFR


16, 26, 49, 117, 190

EP32 S100 Beta


17, 27, 50, 127, 190

EP3372 Factor XIII A


14, 25, 47, 97, 188

EP36 Bcl-2


11, 23, 43, 55, 184

EP38Y EGFR(PAN)


14, 25, 47, 93

EP4 Cytokeratin 4


13, 25, 46, 84, 187

EP449E CD3 Epsilon AN477-10M 12, 23, 44, 62, 185

EP454Y Cyclin E2


13, 25, 46, 84, 187

EP5 Ki-67


14, 25, 48, 95, 188

EP54 CD146


13, 24, 45, 80, 186

EP56 CD90


13, 24, 45, 78, 186

EP6 E-Cadherin


14, 25, 47, 93, 187

EP798Y Calponin-1 (C-term)


11, 23, 43, 60, 184

EP82 CD79a


13, 24, 45, 77, 186

EP9 p53


16, 26, 49, 120, 190

EPR1023 Mucin 1 (MUC1)


15, 26, 48, 112, 189

223


[email protected]


EPR1344 CD11b/ITAM


12, 24, 44, 66, 185

EPR1600Y Cytokeratin 5


13, 25, 46, 85

EPR1600Y + EPR1602Y Cytokeratin 5 & 6


13, 25, 46, 85, 187



13, 25, 46, 85, 187



14, 25, 46, 88



14, 25, 46, 86, 187



14, 25, 46, 89, 187

EPR1792Y N-Cadherin


16, 26, 49, 116, 189

EPR2241(IHC)-32 Cyclin D1


13, 24, 46, 84, 187

EPR3097Y CD99


13, 24, 45, 78, 186

EPR3159Y TFPU.1


17, 27, 52, 130, 191

EPR3432 PDCD4


16, 27, 49, 122, 190

EPR3611 Ki-67


15, 26, 48, 107, 189

EPR3653 CD14


12, 24, 44, 65, 185

EPR3947 PMS2


16, 27, 49, 124, 190

EPR542 Oct 2


16, 26, 49, 117, 190

EPR703(2) Estrogen Receptor (ER Alpha)


14, 25, 47, 96

ER88 Estrogen Receptor (InSite® ER)


14, 25, 47, 96, 188


ErPr8 Prostate Specific Antigen(PSA)


16, 27, 50, 126, 190

ES05 MLH1


15, 26, 48, 111, 189

F14 Fibronectin


14, 25, 47, 98, 188

F39.4.1 Androgen Receptor


11, 23, 43, 53, 184

F8 2.2.9 Factor VIII-Related antigen


14, 25, 47, 97, 188

FCN01 Fascin


14, 25, 47, 97, 188

FPC1 CD22AM439-5M, AX439-YCD, MU439-UC

12, 44, 69, 185

G175-405 p16 (INK4a)AM540-5M, AM540-10M, MU540-UC

26, 49, 118, 190

G3.1Heat Shock Protein 27 (HSP 27)


13, 26, 47, 101, 188

GA-5Glial Fibrillary Acidic Protein (GFAP)

AM020-10M 14, 25, 47, 99, 188

gp100/D5 Melanoma gp100


15, 26, 48, 110, 189

GPC3-88 Glypican-3 (GPC3)AM539-5M, AM539-10M, MU539-UC

25, 47, 100, 188

H68.4 CD71 (Transferrin Receptor)


13, 45, 77

HB125 Insulin

AM029-5M, AW029-YCD, AX029-YCD, MU029-UC

15, 48, 105, 189

h-CDCaldesmon, High MW, Smooth muscle


11, 43, 59

HHF35 Actin, Muscle-Specific


11, 23, 43, 51, 184

HMB45 Melanoma


15, 26, 48, 109, 189

HO36.1.1 CD99 (MIC2 Antigen)


13, 24, 45, 78

HRS-4 CD30 (Ki-1 Antigen)


12, 24, 44, 70

224


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HSA/E8 HSA


15, 25, 48, 103, 188

HT1/13.6.3 Transferrin AM025-5M, AX025-YCD 17, 50, 132, 191

HWD1.1 Collagen IIIAM167-5M, AX167-YCD, MU167-UC

13, 48, 83, 187

ID2C3 Villin


17, 27, 50, 134, 191

IgG88 IgGAM367-5M, AX367-YCD, MU367-UC

15, 48, 104, 188

IgM88 IgMAM366-5M, AX366-YCD, MU366-UC

15, 48, 104, 188

IP64 Napsin A


16, 26, 49, 116, 189

IT-Ks20.8 Cytokeratin 20

AM315-5M, AM315-10M, MU315-UC, AX315-YCD

14, 25, 46, 89, 187

JB1a CD29AM298-5M, AX298-YCD, MU298-UC

12, 44, 69, 185

JC/70A CD31AM232-5M, AM232-10M, AX232-YCD

12, 24, 44, 70, 185

JC88 J ChainAM374-5M, AX374-YCD, MU374-UC

15, 48, 106

JDR3B8 Beta-Tubulin II MU176-UC 43, 56, 184

JLN20 Alpha-ActininAM097-5M, AX097-YCD, MU097-UC

11, 43, 52, 184

K-2 Ki-67


15, 26, 48, 106, 189

K88 Kappa Light Chain


15, 26, 48, 106, 189

Ki88 Ki-67 Antigen, Proliferating Cell


15, 26, 48, 107, 189

KP1 CD68


13, 24, 45, 76, 186

L1C1 Kappa Light Chain


15, 26, 48, 106, 189

L-26 CD20 (B Cell)


12, 24, 44, 185

LJ27.9CD45 (Leukocyte common Antigen,LCA)


12, 45, 73

LK2H10 Chromogranin A


13, 24, 45, 82, 186


LL002 Cytokeratin 14


14, 25, 46, 87, 187

LL002+DEK-10+RCK108+OV-TL12/30+C11



14, 25, 46, 90, 187

LN1 CDw75 (B Cell)AM152-5M, AX152-YCD, MU152-UC

13, 45, 80, 186

LN2 CD74 (B Cell)AM153-5M, AX153-YCD, MU153-UC

13, 45, 77, 186

LN22 Bcl-6


11, 23, 43, 55

LN3 HLA-DRAM154-5ME, AX154-YCDE, MU154-UCE

15, 47, 103, 188

LN5 MacrophageAM165-5M, AX165-YCD, MU165-UC

15, 48, 109, 189

LN6 Vimentin,Non-Hematopoietic


17, 50, 134, 191

LO26 Myf4


16, 26, 49, 114, 189

LP15 CD7


12, 23, 44, 64, 185

Lu-5 Cytokeratin, PanAM181-5M, AX181-YCD, MU181-UC

14, 46, 91, 187

M01 CD11b (C3bi Receptor)

AM270-5M, AX270-YCD 12, 44, 66

M94138Human Chorionic Gonadotropin (hCG) Beta


15, 48, 103

MB1 CD45RA (B Cell)AM157-5M, AM157-10M, AX157-YCD

12, 24, 45, 73, 186

MB2 B Cell


11, 43, 54, 184

MBP88 Myelin Basic Protein

AM380-5M, AM380-10M, AX380-YCD

16, 26, 48, 113

Mc5Epithelial Membrane Antigen (EMA)


14, 25, 47, 94, 187

MDR88Multi-Drug Resistance Marker (P-Glycoprotein)


15, 48, 113

ME.121 Prolactin


16, 28, 49, 125

MEM55 CD45RBAM320-5M, AX320-YCD, MU320-UC

12, 45, 73, 186

MEM55+LJ27.9 CD45 Cocktail (Leukocyte Antigen, LCA)


12, 24, 45, 73, 186

225


[email protected]


MG-1 MyoglobinAM012-5M, AX012-YCD, MU012-UC

16, 49, 115, 189

MIB-1 Ki-67 Antigen, Proliferating Cell


15, 26, 48, 107, 189

MIG-N3 Neuron Specific Enolase (NSE)


16, 26, 49, 116, 190

MiTF/A13 MiTF


15, 26, 48, 110, 189

MOC-31 Epithelial-Specific Antigen

AM316-5M, AM316-10M, MU316-UC, AX316-YCD

14, 25, 47, 94, 188

Mouse 4A4 + Mouse34BE12 + Rabbit13H4

PIN4 (p63+CKHMW+ P504S)


16, 27, 147,190

Mouse 4A4 + Rabbit EPR1600Y p63 + CK5

AC602-5M, AC602-10M, AC602-YCD

16, 27, 147

Mouse 4B12 + Rabbit EP1150Y CD4 + C8 Cocktail

AC595-5M, AC595-10M, AC595-YCD

12, 23, 145

Mouse 56C6 + Rabbit EPR2241 (IHC)-32

CD10 and Cyclin D1 cocktail

AC561-5M, AC561-10M, AC561-YCD

12, 24, 145, 185

Mouse 7C7-D4 + Rabbit EPR1619Y

CDX-2+CK7 cocktail

AC596-5M,AC596-10M, AC596-YCD

13, 24, 145, 186

Mouse 7C7-D4 + Rabbit EPR1622Y+ EPR1619Y

CDX-2+CK20+CK7 cocktail

AC597-5M AC597-10M, AC597-YCD

13, 24, 147

Mouse 8G7G3/1 + Rabbit EP1582Y

TTF-1+GCDFP-15 cocktail

AC604-5M, AC604-10M, AC604-YCD

17, 27, 146, 191

Mouse 8G7G3/1 + Rabbit EP1582Y + EP1619Y

TTF-1+CK20+CK7 cocktail

AC605-5M, AC605-10M, AC605-YCD

17, 27, 147

Mouse 8G7G3/1 + Rabbit EPR1600Y + Rabbit1602Y

TTF1 and Cytokeratin 5/6 cocktail

AC563-5M, AC563-10M, AC563-YCD

17, 27, 146, 191

Mouse G175-405 + Rabbit EPR3611

p16+Ki67 cocktail AC601-5M, AC601-10M 26, 146, 190

Mouse K-2 + Rabbit Poly

Ki-67 and Lambda Light Chain Cocktail

AC562-5M, AC562-10M, AC562-YCD

15, 26, 146, 189

Mouse L26 + Rabbit EP449E

CD3 and CD20 cocktail

AC559-5M, AC559-10M, AC559-YCD

12, 23, 145, 185

MT1 & MB1 CD43 & CD45RA Cocktail

AM159-5M, AX159-YCD 12, 44, 72, 186

MT2 CD45RC (T Cell)AM156-5M, AX156-YCD, MU156-UC

12, 45, 74, 186

MY-32 Myosin,Skeletal Muscle


16, 49, 115, 189

NE-14 Neurofilament


16, 26, 49, 116, 190


NK-1 CD57 (Natural Killer Cell)


13, 45, 75, 186

NKH-1 CD56 (Natural Killer Cell,NCAM)


13, 45, 75

NKI/C3Melanoma-Associated Antigen


15, 26, 48, 110, 189

O10 CD1a


11, 23, 44, 62, 185

ONS1A6 Beta-Tubulin IV MU178-UC 43, 57, 184

OST1 OsteonectinAM387-5M, AX387-YCD, MU387-UC

16, 49, 118

Ov185:1 CA125


11, 23, 43, 59, 184

OV-TL12/30 Cytokeratin 7


14, 25, 46, 86, 187

OV-TL12/30 & C51 Cytokeratin 7&8


14, 25, 46, 86, 187

PC10Proliferating Cell Nuclear Antigen (PCNA)


16, 27, 49, 125, 190

PD7/26/16 & 2B11 CD45

AM111-5M, AM111-10M, AX111-YCD

12, 24, 45, 72, 186

PDGF88Platelet-Derived Growth Factor (PDGF)


16, 49, 123

PHE-5 Chromogranin A


13, 24, 45, 82, 187

PL8-F6Placental Alkaline Phosphatase (PLAP)


16, 27, 49, 123, 190

POH-1p34cdc2 (Cyclin Dependent Kinase)


16, 49, 119, 190

Polyclonal Alpha-1-Antitrypsin


11, 43, 52, 184

Polyclonal Bax Protein AR347-5R, AW347-YCD, PU347-UP

11, 43, 54

Polyclonal BRCA1 ProteinAR345-5R, AW345-YCD, PU345-UP

11, 43, 55

Polyclonal Calretinin

AR413-5R,AR413-10R, AW413-YCD, PU413-UP

11, 23, 60, 184

Polyclonal CD23


12, 24, 44, 69, 185

Polyclonal Carcinoembryonic Antigen (CEA)

AR009-5R, AW009-YCD 11, 43, 61, 185

226


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Polyclonal Cyclin D1


13, 24, 46, 83, 187

Polyclonal EGFRAR335-5RE, AW335-YCDE, PU335-UPE

14, 47, 93

Polyclonal Estrogen Receptor-Beta (ER-β)


14, 25, 47, 96

Polyclonal Estradiol AR038-5R, AW038-YCD 14, 47, 95, 188

Polyclonal Follicle Stimulating Hormone (FSH)

AR026-5R, AW026-YCD 14, 47

Polyclonal GastrinAR019-5R, AW019-YCD, PU019-UP

14, 47, 98, 188

PolyclonalGlial Fibrillary Acidic Protein (GFAP)


14, 47, 99, 188

Polyclonal GlucagonAR039-5R, AW039-YCD, PU039-UP

14, 47, 99, 188

Polyclonal Glutathione S-Transferase Pi (GST Pi)

AR249-5R,AW249-YCD, PU249-UP

14, 47, 100, 188

Polyclonal Helicobacter pylori

AR442-5RE, AR442-10RE, AW442-YCDE, PU442-UPE

15, 26, 47, 101

Polyclonal Hemoglobin A AR021-5R, AW021-YCD 15, 47, 102, 188

Polyclonal Hepatitis B Virus Core Antigen (HBcAg)

AR082-5RE, PU082-UPE 47, 102

Polyclonal Placental Lactogen (hPL)


16, 27, 49, 123, 190

Polyclonal Herpes Simplex Virus Type I (HSV I)

AR084-5RE,AW084-YCDE, PU084-UPE

15, 47, 102

Polyclonal Herpes Simplex Virus Type II (HSV II)


15, 47, 102

Polyclonal IgAAR045-5R, AW045-YCD, PU045-UP

15, 48, 103

Polyclonal IgGAR050-5R, AW050-YCD, PU050-UP

15, 48, 104, 188

Polyclonal IgM


15, 48, 105, 188

Polyclonal Lambda Light Chain


15, 48, 108, 189

Polyclonal Laminin


15, 26, 48, 108, 189

Polyclonal lgDAR440-5R, PU440-UP, AW440-YCD

15, 48, 104, 188


Polyclonal Lysozyme


15, 26, 48, 108, 189

Polyclonal Myeloperoxidase (MPO)


16, 26, 49, 114, 189

Polyclonal Myoglobin


16, 49, 115, 189

PolyclonalPlatelet-Derived Growth Factor (PDGF)


16, 49, 124

Polyclonal S-100 Protein


17, 27, 50, 127, 190

Polyclonal SecretinAR067-5R,AW067-YCD

17, 50, 128, 191

Polyclonal Substance PAR069-5R, AW069-YCD, PU069-UP

17, 50, 128, 191

PolyclonalTerminal Deoxynucleotidyl Transferase (TdT)

AR373-5R, AW373-YCD 17, 50, 130, 191

Polyclonal Toxoplasma gondii AR125-5RE, PU125-UPE 50, 132

PolyclonalThyroid Stimulating Hormone (TSH)

AR033-5R, AW033-YCD 17, 50, 131

Polyclonal VEGF


17, 27, 50, 134, 191

Polyclonal VIP


17, 27, 50, 135, 191

Polyclonal Catenin Delta 1 (p120)


11, 23, 44, 61, 185

Polyclonal Claudin 1


13, 24, 45, 82, 187

Polyclonal HGH


15, 26, 47, 101, 188

PR88Progesterone Receptor (InSite®PR)


16, 27, 49, 125, 190

PS1 CD3 (T Cell)


12, 23, 44, 63, 185

PS2.1 pS2 Estrogen Inducible Protein


17, 27, 50, 126, 190

QBend/10 CD34 (Endothelial Cell)


12, 24, 44, 71, 185

227


[email protected]


R1 Inhibin-Alpha


15, 26, 48, 105, 189

RBT-AMACR P504S (AMACR)


16, 26, 49, 120, 190

RCC-26 RCC (Renal Cell Carcinoma)


17, 27, 50, 126, 190

RCK108 Cytokeratin 19


14, 25, 46, 89, 187

RLB25 CD35


12, 24, 44, 71, 185

RTJ1/A2 c-erbB-3 (HER-3)AM319-5M, AX319-YCD, MU319-UC

13, 24, 45, 81

SDL3D10 Beta-Tubulin III MU177-UC 43, 56, 184

SMMS.1Myosin Heavy Chains, Smooth Muscle


16, 26, 49, 115

Snp88 Synaptophysin


17, 27, 50, 129, 191

SP16 CD8


12, 23, 44, 65

SP191 CD35

AN741-5M, AN741-10M, AY741-YCD, NU741-YCD

12, 24, 44, 71

SP46 MSH2


15, 26, 48, 111

SP92 BOB1


11, 23, 43, 58

SPM498 GLUT-1


14, 25, 47, 100, 188

T595 CD117


13, 24, 45, 79, 186

T8CD8 (T Cell,Suppressor/Cytotoxic)


12, 44, 65

T9 CD71 (Transferrin Receptor)

AM269-5M, AX269-YCD 13, 45, 76

TAU-2 Tau


17, 27, 50, 129, 191

Tau-5 Tau


17, 27, 50, 129, 191


TdT88Terminal Deoxynucleotidyl Transferase (TdT)


13, 27, 50, 130

TGF88Transforming Growth Factor (TGF),Alpha


17, 50, 133

Ty/G5 Tyrosinase


17, 27, 50, 133, 191

UCHL-1 CD45RO (T Cell)


13, 24, 45, 74, 186

UCHT1 CD3 (T Cell)AM258-5M, AX258-YCD, MU258-UC

12, 44, 63

V9 Vimentin


17, 27, 50, 134, 191

VU-4H5 CD227 (MUCIN 1)


15, 26, 45, 80, 186

Y113 Paxillin (N-Term)


16, 27, 49, 122, 190

Y236 p27 (Kip1)


16, 26, 49, 119, 190

Y92 PDGF Receptor-ß


16, 27, 49, 122, 190

YE291 ZAP-70


17, 27, 50, 135, 191

YE372 Catenin (p120)


16, 26, 44, 61, 185

YR145 CD117


13, 24, 45, 79, 186

ZAP70-C3 ZAP-70


17, 27, 50, 135, 191

ZMSA-5 Sarcomeric Actin


17, 27, 50, 128, 190

ZP007 PAX-5


16, 27, 49, 122, 190

228

Listing by Categories

www.biogenex.com

Categories Clone Page

ACUTE MYELOID LEUKEMIA

CD34 (Endothelial Cell) QBend/10(M) 12, 24, 44, 71, 185

Myeloperoxidase (MPO) Polyclonal(R) 16, 26, 49, 114, 189

ADHESION MOLECULES

Beta-Catenin E247(R) 11, 23, 43, 56 ,184

CD11b/ITAM EPR1344(R) 12, 24, 44, 66, 185

CD22 FPC1(M) 12, 44, 69, 185

CD31 (PECAM-1) 9G11(M) 12, 44, 70, 185

CD44 (Phagocytic Glycoprotein-1,HCAM) DF1485(M) 12, 45, 72, 186

CD61 EP2417Y(R) 13, 24, 45, 75, 186

E-Cadherin 36(M) 14, 25, 47, 93

E-Cadherin EP6(R) 14, 25, 47, 93, 187

Ep-CAM E144(R) 14, 25, 47, 94, 188

N-Cadherin EPR1792Y(R) 16, 26, 49, 116, 189

ADRENAL TUMORS

CD56 (Natural Killer Cell,NCAM) NKH-1(M) 13, 45, 75

Inhibin-Alpha R1(M) 15, 26, 48, 105, 189

Synaptophysin Snp88(M) 17, 27, 50, 129, 191

AMYLOID

Kappa Light Chain L1C1(M) 15, 26, 48, 106, 189

Kappa Light Chain K88(M) 15, 26, 48, 106, 189

Lambda Light Chain Polyclonal(R) 15, 48, 108, 189

Lambda Light Chain EP172(R) 15, 26, 48, 108, 189

BLADDER


c-erbB-2 (HER-2/neu) EP3(R) 13, 24, 45, 81

Cytokeratin 20 IT-Ks20.8(M) 14, 25, 46, 89, 187

Cytokeratin, High MW (Basic) AE3(M) 14, 46, 90, 187

Cytokeratin, Low MW AE1(M) 14, 25, 46, 91, 187

Ki-67 K-2(M) 15, 26, 48, 106, 189

Ki-67 Antigen, Proliferating Cell MIB-1(M) 15, 26, 48, 107, 189

Ki-67 Antigen, Proliferating Cell Ki88(M) 15, 26, 48, 107, 189

p53 EP9(R) 16, 26, 49, 120, 190

p53 Protein BP53-12-1(M) 16, 26, 49, 120, 190

p53 Protein DO7(M) 16, 27, 49, 121, 190

p53 Protein 1801(M) 16, 27, 49, 121, 190

MMP-9 EP1255Y(R) 15, 26, 48, 111, 189

BLOOD GROUP ANTIGEN

Blood Group Antigen Lewis A 7LE(M) 43, 57, 184

Blood Group Antigen Lewis B 2-25LE(M) 43, 57, 184

BRAIN PATHOLOGY

Apolipoprotein E EP1373Y(R) 11, 23, 43, 53, 184

Epithelial Membrane Antigen (EMA) E29(M) 14, 25, 47, 94, 187

Epithelial Membrane Antigen (EMA) Mc5(M) 14, 25, 47, 94, 187

Follicle Stimulating Hormone (FSH) Polyclonal(R) 14, 47

Human Chorionic Gonadotropin (hCG) Beta M94138(M) 15, 48, 103

HGH Polyclonal(R) 15, 26, 47, 101, 188


BREAST PANEL

Androgen Receptor F39.4.1(M) 11, 23, 43, 53, 184

Bax Protein Polyclonal(R) 11, 43, 54

Bcl-2 E17(R) 11, 23,43, 54, 184

Bcl-2 Oncoprotein Bcl-2/100(M) 11, 23, 43, 55, 184

Beta-Catenin E247(R) 11, 23, 43, 56, 184

BRCA1 Protein Polyclonal(R) 11, 43, 55

Breast Cancer Antigen BCA 225 CU18(M) 11, 23, 43, 58, 184

CA 19-9 C241:5:1:4(M) 11, 23, 43, 58, 184

Calponin CALP(M) 11, 43, 59, 184

Calponin-1 (C-term) EP798Y(R) 11, 23, 43, 60, 184

Catenin (p120) YE372(R) 16, 26, 44, 61, 185

Catenin Delta 1 (p120) Polyclonal(R) 11, 23, 44, 61, 185

Cathepsin D C15(M) 11, 44, 62


CD66 BY114(M) 13, 45, 76, 186

CD117 T595(M) 13, 24, 45, 79, 186

CD117 YR145(R) 13, 24, 45, 79, 186

CD227 (MUCIN 1) VU-4H5(M) 15, 26, 45, 80, 186

CDX-2+CK20+CK7 cocktailCDX2-88(M)+ EPR1622Y(R)+ EPR1619Y(R)

13, 24, 147

CDX-2+CK7 cocktailCDX2- 88(M)+EPR1619Y( R)

13, 24, 145, 186

c-erbB-2 (HER-2/neu) CB11(M) 13, 24, 45, 81

c-erbB-2 (HER-2/neu) EP3(R) 13, 24, 45, 81

c-erbB-3 (HER-3) RTJ1/A2(M) 13, 24, 45, 81

Cytokeratin 5 EPR1600Y(R) 13, 25, 46, 85

Cytokeratin 5 & 6 EPR1600Y & EPR1602Y(R) 13, 25, 46, 85, 187

Cytokeratin 5 & Cytokeratin 14 EP24/EP61(R) 13, 25, 46, 85

Cytokeratin 7 OV-TL12/30 14, 25, 46, 86, 187

Cytokeratin 7 & 8 OV-TL12/30 & C51(M) 14, 25, 46, 86, 187

Cytokeratin 8 C-51(M) 14, 25, 46, 86, 187

Cytokeratin 14 LL002(M) 14, 25, 46, 87, 187


EGFR Polyclonal(R) 14, 47, 93

EGFR(PAN) EP38Y(R) 14, 25, 47, 93

E-Cadherin EP6(R) 14, 25, 47, 93, 187

E-Cadherin 36(M) 14, 25, 47, 93

erbB-2/HER-2 EP1045Y(R) 13, 24, 47, 95

Estrogen Receptor 1D5(M) 14, 25, 47, 95, 188

Estrogen Receptor EP1(R) 14, 25, 47, 96

Estrogen Receptor (ER Alpha) EPR703(2)(R) 14, 25, 47, 96

Estrogen Receptor (InSite® ER) ER88(M) 14, 25, 47, 96, 188

Estrogen Receptor-Beta (ER-β) Polyclonal(R) 14, 25, 47, 96

Fascin FCN01(M) 14, 25, 47, 97, 188

GCDFP-15 EP1582Y(R) 14, 25, 47, 98, 188

Macrophage LN5(M) 15, 48, 109, 189

MMP-9 EP1255Y(R) 15, 26, 48, 111, 189

MUC4 1G8(M) 15, 26, 48, 112, 189

229


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Mucin 1 (MUC1) EPR1023(R) 15, 26, 48, 112, 189

Mucin 2 (MUC2) CCP58(M) 15, 48, 113

Myosin Heavy Chains,Smooth Muscle SMMS.1(M) 16, 26, 49, 115

p53 EP9(R) 16, 26, 49, 120, 190

p53 Protein BP53-12-1(M) 16, 26, 49, 120, 190

p53 Protein DO7(M) 16, 27, 49, 121, 190

p53 Protein 1801(M) 16, 27, 49, 121, 190

p63 4A4(M) 16, 27, 49, 121, 190

p63+CK5 Cocktail 4A4(M)+ EPR1600Y( R) 16, 27, 147

Progesterone Receptor EP2(R) 16, 27, 49, 124

Progesterone Receptor 1A6(M) 16, 27, 49, 124

Progesterone Receptor (InSite®PR) PR88(M) 16, 27, 49, 125, 190

Topo 2 Alpha EP1102Y(R) 17, 27, 50, 132, 191

TTF-1+CK20+CK7 cocktail8G7G3/1(M) + EPR1622Y(R) + EPR1619Y(R)

17, 27, 147

TTF-1+GCDFP-15 cocktail 8G7G3/1(M) + EP1582Y(R) 17, 27, 146, 191

Tumor-Associated Glycoprotein (TAG-72) B72.3(M) 17, 27, 50, 133, 191

Tumor-Associated Glycoprotein (TAG-90 BCA) B6.2(M) 17, 50, 133, 191

CELL SURFACE MARKERS

DOG1 1.1(M) 14, 25, 46, 92, 187

Glut-1 SPM498(M) 15, 25, 47, 100, 188

CELLULAR ANTIGENS

Alpha-1-Antitrypsin Polyclonal(R) 11, 43, 52, 184

Cyclin D1 Polyclonal(R) 13, 24, 46, 83, 187

Cyclin D1 EPR2241(IHC)-32(R) 13, 24, 46, 84, 187

Cyclin E2 EP454Y(R) 13, 25, 46, 84, 187

Dystrophin Dys1(Dy4/6D3)(M) 14, 46, 92, 187

Dystrophin Dys2(Dy8/6C5)(M) 14, 47, 92, 187

Glomerular Epithelial Protein 1 (GLEPP-1) 5C11(M) 14, 47, 99

Mitochondrial Antigen 113-1(M) 48, 111

Myelin Basic Protein MBP88(M) 16, 26, 48, 113

Myoglobin MG-1(M) 16, 49, 115, 189

Myoglobin Polyclonal(R) 16, 49, 115, 189

Myosin,Skeletal Muscle MY-32(M) 16, 49, 115, 189

p27 (Kip1) DCS72(M) 16, 49, 119

p27 (Kip1) Y236(R) 16, 26, 49, 119, 190

PAX-5 ZP007(M) 16, 27, 49, 122, 190

PDGF Receptor-ß Y92(R) 16, 27, 49, 122, 190

PMS2 EPR3947(R) 16, 27, 49, 124, 190

Survivin EP2880Y(R) 17, 27, 50, 129, 191

Transferrin HT1/13.6.3(M) 17, 50, 132, 191

CERVICAL

Carcinoembryonic Antigen (CEA) B01-94-11M-P(M) 11, 23, 43, 60, 185

Carcinoembryonic Antigen (CEA) CEA88(M) 13, 23, 43, 61, 185

Carcinoembryonic Antigen (CEA) Polyclonal(R) 11, 43, 61, 185

c-myc Protein 9E 10(M) 13. 45, 83, 187



Cytokeratin 7 EPR1619Y(R) 14, 25, 46, 86, 187


Cytokeratin 10 DEK-10(M) 14, 46, 87, 187

Cytokeratin 13 AE8(M) 14, 46, 87, 187

Cytokeratin 17 E27(M) 14, 25, 46, 88, 187

Cytokeratin 19 RCK108(M) 14, 25, 46, 89, 187



Herpes Simplex Virus Type I (HSV I) Polyclonal 15, 47, 102

Herpes Simplex Virus Type II (HSV II) Polyclonal(R) 15, 47, 102

Ki-67 EP5(R) 14, 25, 48, 95, 188

Ki-67 K-2(M) 15, 26, 48, 106, 189

Ki-67 EPR3611(R) 15, 26, 48, 107, 189

Ki-67 Antigen, Proliferating Cell MIB-1(M) 15, 26, 48, 107, 189

Ki-67 Antigen, Proliferating Cell Ki88(M) 15, 26, 48, 107, 189

p16 (INK4a) G175-405(M) 26, 49, 118, 190

p16+Ki67 cocktail "G175-405(M) +EPR3611( R)" 26, 146, 190

p63 4A4(M) 16, 27, 49, 121, 190

Papillomavirus Type 16 (HPV-16) Cam Vir-1 16, 49, 121

CNS TUMORS

NGF Receptor EP1039Y(R) 16, 26, 49, 117, 190

NGF Receptor EP31(R) 16, 26, 49, 117, 190

COLON

Bcl-2 E17(R) 11, 23, 43, 54, 184

Bcl-2 Oncoprotein bcl-2/100(M) 11, 23, 43, 55, 184

Beta-Catenin E247(R) 11, 23, 43, 56, 184


13, 24, 147


13, 24, 145, 186

Fascin FCN01(M) 14, 25, 47, 97, 188

P504S (AMACR) 13H4(R) 16, 26, 49, 120, 190

P504S (AMACR) RBT-AMACR(R) 16, 26, 49, 120, 190

COLORECTAL PANEL

CA19-9 C241:5:1:4(M) 11, 23, 43, 58, 184

CA 125 (Ovarian Tumor Marker) Ov185:1(M) 11, 23, 43, 59, 184




CD10 56C6(M) 12, 23, 44, 65, 185

CDX-2 CDX2-88(M) 13, 24, 45, 81, 186

c-myc Protein 9E 10(M) 13. 45, 83, 187






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Cytokeratin Cocktail AE1 and AE3(M) 14, 25, 46, 89, 187


EGFR(PAN) EP38Y(R) 14, 25, 47, 93

Glut-1 SPM498(M) 15, 25, 47, 100, 188

MLH1 ES05(M) 15, 26, 48, 111, 189

MMP-9 EP1255Y(R) 15, 26, 48, 111, 189

MSH2 SP46(R) 15, 26, 48, 111

MSH6 2D4B5(M) 15, 26, 48, 112 ,189

MUC5AC 45M1(M) 15, 26, 48, 112, 189

Mucin 1 (MUC1) EPR1023(R) 15, 26, 48, 112, 189

Mucin 2 (MUC2) CCP58(M) 15, 48, 113

p21/WAF1 4D10(M) 16, 49, 119, 190

p53 EP9(R) 16, 26, 49, 120, 190

p53 Protein BP53-12-1(M) 16, 26, 49, 120, 190

p53 Protein DO7(M) 16, 27, 49, 121, 190

p53 Protein 1801(M) 16, 27, 49, 121, 190

PMS2 EPR3947(R) 16, 27, 49, 124, 190

Villin ID2C3(M) 17, 27, 50, 134, 191

CYTOTOXIC DRUG METABOLISM

Glutathione S-Transferase Pi (GST Pi) Polyclonal(R) 14, 47, 100, 188

Multi-Drug Resistance Marker (P-Glycoprotein) MDR88(M) 15, 48, 113

EMBRYONAL CARCINOMA

Alpha-Fetoprotein (AFP) C3(M) 11, 23, 43, 53, 184

ENDOCRINE PANEL

ACTH AH26(M) 11, 23, 43, 51, 184

Estradiol Polyclonal(R) 14, 47, 95, 188

Follicle Stimulating Hormone (FSH) Polyclonal(R) 14, 47

Glucagon Polyclonal(R) 14, 47, 99, 188

HGH Polyclonal(R) 15, 26, 47, 101, 188


Inhibin-Alpha R1(M) 15, 26, 48, 105, 189

Insulin EP125(R) 15, 26, 48, 106, 189

Insulin HB125(M) 15, 48, 105, 189

Prolactin ME.121(M) 16, 28, 49, 125

Thyroglobulin 2H11(M) 17, 27, 50, 130, 191

Thyroid Stimulating Hormone (TSH) 5404(M) 17, 27, 50, 131

Thyroid Stimulating Hormone (TSH) Polyclonal(R) 17, 50, 131

Thyroxine D5(M) 17, 50, 131, 191

ENDOMETRIOID CARCINOMA

Vimentin V9(M) 17, 27, 50, 134, 191

ENDOTHELIAL VASCULAR MARKER

CD31 (Endothelial Cell) JC/70A(M) 12, 24, 44, 70, 185

CD31 (PECAM-1) 9G11(M) 12, 44, 70, 185


Factor VIII-Related antigen F8 2.2.9(M) 14, 25, 47, 97, 188

Factor XIII Subunit A E980.1(M) 14, 25, 47, 97, 188

Factor XIII A EP3372(R) 14, 25, 47, 97, 188


ENZYMES

Alpha-1-Antichymotrypsin α1A88(M) 11, 43, 52



EPITHELIAL MARKERS


Cytokeratin 4 EP4(R) 13, 25, 46, 84, 187

Cytokeratin 4 6B10(M) 13, 25, 46, 84, 187


Cytokeratin 8 & 18 5D3(M) 14, 25, 46, 87, 187

Cytokeratin 18 DC-10(M) 14, 46, 88, 187



34ßE12/C51/AE1(M) 14, 46, 90, 187

Cytokeratin cocktail, Broad Spectrum CK88(M) 14, 25, 46, 90, 187

Cytokeratin, High MW 34ßE12(M) 14, 25, 46, 90, 187



Cytokeratin, Pan Lu-5(M) 14, 46, 91, 187

Cytokeratin, Pan C11(M) 14, 46, 91

Collagen IV COL-94(M) 14, 46, 83

Ep-CAM E144(R) 14, 25, 47, 94, 188

Epithelial-Specific Antigen MOC-31(M) 14, 25, 47, 94, 188

Glomerular Epithelial Protein 1 (GLEPP-1) 5C11(M) 14, 47, 99

MUC4 1G8(M) 15, 26, 48, 112, 189

Mucin 2 (MUC2) CCP58(M) 15, 48, 113

EWING'S SARCOMA


EXTRACELLULAR MATRIX PROTEIN

Collagen III HWD1.1(M) 13, 48, 83, 187


Fibronectin F14(R) 14, 25, 47, 98, 188

Laminin Polyclonal(R) 15, 26, 48, 108, 189

Osteonectin OST1(M) 16, 49, 118

Nuclear Ribonucleoprotein 58-15(M) 49, 117, 190

GASTROINTESTINAL PANEL




CD117 T595(M) 13, 24, 45, 79, 186

CD117 YR145(R) 13, 45, 79, 186

CDX-2 CDX2-88(M) 13, 24, 45, 81, 186

c-erbB-2 (HER-2/neu) EP3(R) 13, 24, 45, 81


Cytokeratin 17 E27(M) 14, 25, 46, 88, 187

DOG1 1.1(M) 14, 25, 46, 92, 187

Secretin Polyclonal(R) 17, 50, 128, 191

Substance P Polyclonal(R) 17, 50, 128, 191

Transforming Growth Factor (TGF),Alpha TGF88(M) 17, 50, 133

231


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TTF-1+CK20+CK7 cocktail“8G7G3/1+ EPR1622Y+ EPR1619Y”

17, 27, 147

GERM CELL TUMORS


Glypican-3 (GPC3) GPC3-88(M) 25, 47, 100, 188

CD30 (Ki-1 Antigen) HRS-4(M) 12, 24, 44, 70

CD117 T595(M) 13, 24, 45, 79, 186

CD117 YR145(R) 13, 45, 79, 186


Oct 4 EP143(R) 16, 28, 49, 118, 190

Placental Alkaline Phosphatase (PLAP) PL8-F6(M) 16, 27, 49, 123, 190

GYNECOLOGICAL

Placental Alkaline Phosphatase (PLAP) PL8-F6(M) 16, 27, 49, 123, 190

Placental Lactogen (hPL) Polyclonal(R) 16, 27, 49, 123, 190

HEMANGIOBLASTOMA

CD31 (PECAM-1) 9G11(M) 12, 44, 70, 185

CD34 (Endothelial Cell) QBend/10(M) 12, 24, 44, 71,185

HEMATOPOIETIC / LYMPHOID MARKERS

CD41/Integrin Alpha IIb EP178(R) 12, 24, 44, 71

CD53 EP179(R) 13, 24, 45, 74,

Granulocyte BM-2(M) 47, 100, 188

Hemoglobin A Polyclonal(R) 15, 47, 102, 188

IgA Polyclonal(R) 15, 48, 103

IgD Polyclonal(R) 15, 48, 104, 188

IgG IgG88(R) 15, 48, 104, 188

IgG Polyclonal(R) 15, 48, 104, 188

IgM IgM88(M) 15, 48, 104, 188

IgM Polyclonal(R) 15, 48, 105, 188

HODGKIN'S LYMPHOMA

Bcl-6 LN22 11, 23, 43, 55

BOB-1 SP92(R) 11, 23, 43, 58

CD15 (Blood group antigen Lewis X) BRA4F1(M) 12, 24, 44, 67, 185

CD30 (Ki-1 Antigen) Ber-H2(M) 12, 24, 44, 70, 185

CD30 (Ki-1 Antigen) HRS-4(M) 12, 24, 44, 70

Fascin FCN01(M) 14, 25, 47, 97, 188

Oct 2 EPR542(R) 16, 26, 49, 117, 190

TFPU.1 EPR3159Y(R) 17, 27, 52, 130, 191

HORMONE REGULATED PROTEIN


Gastrin Polyclonal(R) 14, 47, 98, 188

Heat Shock Protein (HSP-70) BRM-22(M) 15, 47, 101, 188

Heat Shock Protein 27 (HSP 27) G3.1(M) 13, 25, 47, 101, 188

pS2 Estrogen Inducible Protein PS2.1(M) 17, 27, 50, 126, 190

Secretin Polyclonal(R) 17, 50, 128, 191

IMMUNOGLOBULIN & COMPLEMENT PROTEINS

IgA Polyclonal(R) 15, 48, 103


IgG IgG88(R) 15, 48, 104, 188


IgM IgM88(M) 15, 48, 104, 188


J Chain JC88(M) 15, 48, 106





INFECTIOUS AGENTS

Adenovirus A62020069P(M) 11, 43, 52

Cytomegalovirus (CMV) BM204(M) 46, 91

Epstein-Barr Virus Early Antigen 1108-1(M) 47, 95, 188

Helicobacter pylori Polyclonal(R) 15, 26, 47, 101

Hepatitis B Virus Core Antigen (HBcAg) Polyclonal(R) 47, 102

Herpes Simplex Virus Type I (HSV I) Polyclonal(R) 15, 47, 102

Papillomavirus Type 16 (HPV-16) CamVir-1(M) 16, 49, 121

Toxoplasma gondii Polyclonal(R) 50, 132

INTERMEDIATE FILAMENTS & CYTOSKELETAL PROTEINS

Actin, Muscle-Specific HHF35(M) 11, 23, 43, 51, 184

Actin, Smooth Muscle 1A4(M) 11, 23, 43, 51, 184

Alpha-Actinin JLN20(M) 11, 43, 52, 184

Alpha-Tubulin DM-1A(M) 43, 53, 184

Beta-Tubulin DM-1B(M) 43, 56, 184

Beta-Tubulin II JDR3B8(M) 43, 56, 184

Beta-Tubulin III SDL3D10(M) 43, 56, 184

Beta-Tubulin IV ONS1A6(M) 43, 57, 184

Caldesmon E89(R) 11, 23, 43, 59, 184

Caldesmon, High MW, Smooth muscle h-CD(M) 11, 43, 59



Desmin D33(M) 14, 28, 46, 92,187



Fascin FCN01(M) 14, 25, 47, 97, 188

Glial Fibrillary Acidic Protein (GFAP) GA-5(M) 14, 25, 47, 99, 188

Glial Fibrillary Acidic Protein (GFAP) Polyclonal(R) 14, 47, 99, 188

Muscle Actins Actin 88 Cocktail(M) 16, 48, 113

Myf4 LO26(M) 16, 26, 49, 114, 189

Myoglobin MG-1(M) 16, 49, 115, 189


Myosin Heavy Chains, Smooth Muscle SMMS.1(M) 16, 26, 49, 115

Myosin, Skeletal Muscle MY-32(M) 16, 49, 115, 189

Neurofilament NE-14(M) 16, 26, 49, 116, 190

Osteonectin OST1(M) 16, 49, 118

Paxillin (N-Term) Y113(R) 16, 27, 49, 122, 190

Sarcomeric Actin ZMSA-5(M) 17, 27, 50, 128, 190

Tau Tau-2(M) 17, 27, 50, 129, 191

Tau Tau-5(M) 17, 27, 50, 129, 191

Villin ID2C3(M) 17, 27, 50, 134, 191

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Vimentin V9(M) 17, 27, 50, 134, 191

Vimentin,Non-Hematopoietic LN6(M) 17, 50, 134, 191

KIDNEY: RENAL EPITHELIAL TUMORS

CD117 T595(M) 13, 24, 45, 79, 186

CD117 YR145(R) 13, 45, 79, 186

LEUKEMIA

CD61 EP2417Y(R) 13, 24, 45, 75, 186

HLA-DR LN3(M) 15, 47, 103, 188

Lysozyme Polyclonal(R) 15, 26, 48, 108, 189

MMP-9 EP1255Y(R) 15, 26, 48, 111, 189

Myeloid specific Antigen BM-3(M) 16, 48, 114, 189

Myeloid specific Antigen BM-1(M) 16, 48, 114

LIVER

Alpha-1-Antichymotrypsin α1A88(M) 11, 43, 52



Glypican-3 (GPC3) GPC3-88(M) 25, 47, 100, 188

HSA HSA/E8(M) 15, 25, 48, 103, 188

p53 EP9(R) 16, 26, 49, 120, 190

p53 Protein BP53-12-1(M) 16, 26, 49, 120, 190

p53 Protein DO7(M) 16, 27, 49, 121, 190

p53 Protein 1801(M) 16, 27, 49, 121, 190

LUNG

Calretinin 2E7(M) 11, 23, 43, 60, 184

Calretinin Polyclonal(R) 11, 23, 60, 184





CD66 BY114(M) 13, 45, 76, 186


13, 24, 147


13, 24, 145, 186

Chromogranin A LK2H10(M) 13, 24, 45, 82, 186

Chromogranin A PHE-5(M) 13, 24, 45, 82, 187

Claudin-5 EP224(R) 13, 24, 45, 82, 187





Cytokeratin 8 & 18 5D3(M) 14, 25, 46, 87, 187










EGFR(PAN) EP38Y(R) 14, 25, 47, 93



Epithelial-Specific Antigen MOC-31(M) 14, 25, 47, 94, 188

Fascin FCN01(M) 14, 25, 47, 97, 188

Ki-67 EP5(R) 14, 25, 48, 95, 188

Ki-67 K-2(M) 15, 26, 48, 106, 189

Ki-67 EPR3611(R) 15, 26, 48, 107, 189


K-2(M) and Polyclonal (R) 15, 26, 146, 189

Ki-67 Antigen,Proliferating Cell MIB-1(M) 15, 26, 48, 107, 189

Ki-67 Antigen,Proliferating Cell Ki88(M) 15, 26, 48, 107, 189

Mast Cell Tryptase AA1(M) 26, 48, 109, 189

Mesothelin 5B2(M) 15, 26, 48, 110, 189

Napsin A IP64(M) 16, 26, 49, 116, 189

Neuron Specific Enolase (NSE) MIG-N3(M) 16, 26, 49, 116, 190

p21/WAF1 4D10(M) 16, 49, 119, 190

p53 EP9(R) 16, 26, 49, 120, 190

p53 Protein BP53-12-1(M) 16, 26, 49, 120, 190

p53 Protein DO7(M) 16, 27, 49, 121, 190

p53 Protein 1801(M) 16, 27, 49, 121, 190

S100P EP186(R) 17, 27, 50, 127, 190


Thyroid Transcription Factor (TTF-1) 8G7G3/1(M) 17, 27, 50, 131, 191

TTF1 and Cytokeratin 5/6 cocktail8G7G3/1(M) + EPR1600Y(R) + EPR1602Y(R)

17, 27, 146, 191

TTF-1+CK20+CK7 cocktail8G7G3/1(M) + EPR1622Y(R) + EPR1619Y(R)

17, 27, 147


VEGF Polyclonal(R) 17, 27, 50, 134, 191

LYMPHOCYTE DISORDERS

CD57 (Natural Killer Cell) NK-1(M) 13, 45, 75, 186

LYMPHOMA




K-2(M) and Polyclonal(R) 15, 26, 146, 189

Macrophage LN5(M) 15, 48, 109, 189

p34cdc2 (Cyclin Dependent Kinase) POH-1(M) 16, 49, 119, 190


LYMPHOMA (B PANEL)

B Cell MB2(M) 11, 43, 54, 184

B Lymphocyte Antigen 36 (BLA.36) A27-42(M) 11, 23, 43, 54, 184

Bcl-2 EP36(R) 11, 23, 43, 55, 184

BOB-1 SP92(R) 11, 23, 43, 58

CD3 and CD20 cocktail EP449E(R) and L-26(M) 12, 23, 145, 185

233


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CD19 EP169(R) 12, 24, 44, 67, 185

CD20 CD20/C23(M) 12, 24, 44, 67, 185

CD20 (B Cell) L-26(M) 12, 24, 44, 185

CD20 (B Cell) B1(M) 12, 44, 68

CD21 EP3093(R) 12, 24, 44, 68, 185

CD21(B Cell) B2(M) 12, 44, 68

CD22 FPC1(M) 12, 44, 69, 185

CD23 Polyclonal(R) 12, 24, 44, 69, 185

CD25 4C9(M) 12, 24, 44, 69, 185

CD45 (Leukocyte common Antigen,LCA) LJ27.9(M) 12, 45, 73

CD45RA (B Cell) MB1(M) 12, 24, 45, 73, 186

CD79a EP82(R) 13, 24, 45, 77, 186

CD79a 11E 3(M) 13, 24, 45, 77, 186

CDw75 (B Cell) LN1(M) 13, 45, 80, 186


IgM IgM88(M) 15, 48, 104, 188






Oct 2 EPR542(R) 16, 26, 49, 117, 190

PAX-5 ZP007(M) 16, 27, 49, 122, 190

LYMPHOMA (T PANEL)

CD1a O10(M) 11, 23, 44, 62, 185

CD2 AB75(M) 11, 44, 62, 185

CD3 Epsilon EP449E(R) 12, 23, 44, 62, 185

CD3 (T Cell) UCHT1(M) 12, 44, 63

CD3 (T Cell) PS1(M) 12, 23, 44, 63, 185

CD3 and CD20 cocktail EP449E(R) and L-26(M) 12, 23, 145, 185

CD4 EP204(R) 12, 23, 44, 63, 185

CD4 4B12(M) 12, 23, 44, 63, 185

CD4 + C8 Cocktail 4B12(M)+ EP1150Y(R) 12, 23, 145

CD5 4C7(M) 12, 23, 44, 64, 185

CD5 EP2952(R) 12, 23, 44, 64, 185

CD7 LP15(M) 12, 23, 44, 64, 185

CD8 SP16(R) 12, 23, 44, 65

CD8 1A5(M) 12, 23, 44, 64, 185

CD8 (C-Term) EP1150Y(R) 12, 23, 44, 65, 185

CD8 (T Cell,Suppressor/Cytotoxic) T8(M) 12, 44, 65

CD16 2H7(M) 12, 44, 67, 185

CD25 4C9(M) 12, 24, 44, 69, 185

CD43 (T Cell, Leukosialin) DFT-1(M) 12, 44, 72, 186

CD45 (Leukocyte common Antigen,LCA) LJ27.9(M) 12, 45, 73

CD45RC (T Cell) MT2(M) 12, 45, 74, 186

CD45RO (T Cell) UCHL-1(M) 13, 24, 45, 74, 186

CD95 EP208(R) 13, 24, 45, 78

CD99 EPR3097Y(R) 13, 24, 45, 78, 186

CD99 (MIC2 Antigen) HO36.1.1(M) 13, 24, 45, 78


CD103 EP206(R) 13, 24, 45, 79

TIA-1 2G9A10F5(M) 17, 27, 50, 132

VIP Polyclonal(R) 17, 27, 50, 135, 191

LYMPHOMAS & LEUKEMIAS

Bcl-2 E17(R) 11, 23, 43, 54, 184

Bcl-2 Oncoprotein Bcl-2/100(M) 11, 23, 43, 55, 184

Bcl-6 LN22 11, 23, 43, 55

BOB-1 SP92(R) 11, 23, 43, 58

CD10 56C6(M) 12, 23, 44, 65, 185

CD10 and Cyclin D1 cocktail 56C6(M) and EPR2241(R ) 12, 24, 145, 185

CD11b (C3bi Receptor) M01(M) 12, 44, 66

CD11b/ITAM EPR1344(R) 12, 24, 44, 66, 185

CD11C EP1347Y(R) 12, 24, 44, 66, 185

CD14 EPR3653(R) 12, 24, 44, 65, 185

CD29 JB1a(M) 12, 44, 69, 185

CD35 SP191(R) 12, 24, 44, 71

CD35 RLB25(M) 12, 24, 44, 71, 185

CD43 & CD45RA Cocktail MT1 &MB1(M) 12, 44, 72, 186

CD45 (Leukocyte common Antigen,LCA)

PD7/26/16& 2B11(M) 12, 24, 45, 72, 186

CD45 Cocktail (Leukocyte Antigen, LCA) MEM55+LJ27.9 (M) 12, 24, 45, 73, 186

CD45RB MEM55(M) 12, 45, 73, 186

CD48 EP148(R) 13, 24, 45, 74

CD68 KP1(M) 13, 24, 45, 76, 186

CD68 CD68/G2(M) 13, 24, 45, 76, 186

CD71 (Transferrin Receptor) T9(M) 13, 45, 76

CD71 (Transferrin Receptor) H68.4(M) 13, 45, 77

CD74 (B Cell) LN2(M) 13, 45, 77, 186

CD90 EP56(R) 13, 24, 45, 78, 186

CD105 4G11(M) 13, 45, 79, 186

CD117 T595(M) 13, 24, 45, 79, 186

CD117 YR145(R) 13, 45, 79, 186

CD205 EP176(R) 13, 24, 45, 80

Cyclin D1 Polyclonal(R) 13, 24, 46, 83, 187

Cyclin D1 EPR2241(IHC)-32(R) 13, 24, 46, 84, 187

Oct 2 EPR542(R) 16, 26, 49, 117, 190

Terminal Deoxynucleotidyl Transferase (TdT) TdT88(M) 13, 27, 50, 130

Terminal Deoxynucleotidyl Transferase (TdT) Polyclonal(R) 17, 50, 130, 191

ZAP-70 ZAP70-C3(M) 17, 27, 50, 135, 191

ZAP-70 YE291(R) 17, 27, 50, 135, 191

MELANOMA

CD63 EP211(R) 13, 24, 45, 75, 186

CD146 EP54(R) 13, 24, 45, 80, 186

Melan-A (MART-1) A103(M) 15, 26, 48, 109, 189

Melanoma HMB45(M) 15, 26, 48, 109, 189

Melanoma gp100 gp100/D5(M) 15, 26, 48, 110, 189

Melanoma-Associated Antigen NKI/C3(M) 15, 26, 48, 110, 189

MiTF MiTF/A13 15, 26, 48, 110, 189

MMP-9 EP1255Y(R) 15, 26, 48, 111, 189

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S100 beta EP32(R) 17, 27, 50, 127, 190

S100 Protein Polyclonal(R) 17, 27, 50, 127, 190

S100 Protein 15E2E2(M) 17, 27, 50, 127, 190

Tyrosinase Ty/G5(M) 17, 27, 50, 133, 191

MENINGIOMAS

Claudin 1 Polyclonal(R) 13, 24, 45, 82, 187

MERKEL CELL CARCINOMA

E-Cadherin 36(M) 14, 25, 47, 93

E-Cadherin EP6(R) 14, 25, 47, 93, 187

MESOTHELIOMA




Mesothelin 5B2(M) 15, 26, 48, 110, 189

Villin ID2C3(M) 17, 27, 50, 134, 191

Wilm’s Tumor CAN-R9(IHC)-56-2(R) 17, 27, 50, 135, 191

TTF-1 and Cytokeratin 5/6 cocktail8G7G3/1(M) + EPR1600Y(R) + EPR1602Y(R)

17, 27, 146, 191

MYOSARCOMA

Actin, Muscle-Specific HHF35(M) 11, 23, 43, 51, 184

Actin, Smooth Muscle 1A4(M) 11, 23, 43, 51, 184

Alpha-Actinin JLN20(M) 11, 43, 52, 184

Caldesmon E89(R) 11, 23, 43, 59, 184

Caldesmon,High MW,Smooth muscle h-CD(M) 11, 43, 59



Myosin Heavy Chains,Smooth Muscle SMMS.1(M) 16, 26, 49, 115

NEUROBIOLOGY (BRAIN PATHOLOGY)

S100 beta EP32(R) 17, 27, 50, 127, 190

S100 Protein Polyclonal(R) 17, 27, 50, 127, 190

S100 Protein 15E2E2(M) 17, 27, 50, 127, 190

NEUROBLASTOMA


Chromogranin A PHE-5(M) 13, 24, 45, 82, 187

Neurofilament NE-14(M) 16, 26, 49, 116, 190

Neuron Specific Enolase (NSE) MIG-N3(M) 16, 26, 49, 116, 190

PGP9.5 3D9(M) 16, 27, 49, 123


NEUROECTODESMAL TUMOR


NEUROENDOCRINE POLYPEPTIDES (BRAIN PATHOLOGY)

Glial Fibrillary Acidic Protein (GFAP) GA-5(M) 14, 25, 47, 99, 188

Glial Fibrillary Acidic Protein (GFAP) Polyclonal(R) 14, 47, 99, 188

Substance P Polyclonal(R) 17, 50, 128, 191

Tau Tau-2(M) 17, 27, 50, 129, 191

Tau Tau-5(M) 17, 27, 50, 129, 191


NEUROFIBROMA


NATURAL KILLER CELL PANEL


CD57 (Natural Killer Cell) NK-1(M) 13, 45, 75, 186

OVARIAN MARKERS


Bcl-2 E17(R) 11, 23, 43, 54, 154


CA19-9 C241:5:1:4(M) 11, 23, 43, 58, 184



CDX-2 CDX2-88(M) 13, 24, 45, 81, 186






Fascin FCN01(M) 14, 25, 47, 97, 188

Villin ID2C3(M) 17, 27, 50, 134, 191


PANCREATIC MARKERS

Beta-Catenin E247(R) 11, 23, 43, 56 ,184

CA19-9 C241:5:1:4(M) 11, 23, 43, 58, 184







Cytokeratin 17 E27(M) 14, 25, 46, 88, 187


E-Cadherin EP6(R) 14, 25, 47, 93, 187

E-Cadherin 36(M) 14, 25, 47, 93

Fascin FCN01(M) 14, 25, 47, 97, 188

Glucagon Polyclonal(R) 14, 47, 99, 188

Insulin EP125(R) 15, 26, 48, 106, 189

Insulin HB125(M) 15, 48, 105, 189

S100P EP186(R) 17, 27, 50, 127, 190


PECOMA

CD63 EP211(R) 13, 24, 45, 75, 186

PERINEURIOMA


PLACENTAL MARKERS


PROLIFERATION-ASSOCIATED ANTIGENS

Cytokeratin 16 EP1615Y(R) 14, 25, 46, 88


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EGFR(PAN) EP38Y(R) 14, 25, 47, 93

Ki-67 EP5(R) 14, 25, 48, 95, 188

Ki-67 K-2(M) 15, 26, 48, 106, 189

Ki-67 EPR3611(R) 15, 26, 48, 107, 189


K-2(M) and Polyclonal (R) 15, 26, 146, 189

Ki-67 Antigen,Proliferating Cell MIB-1(M) 15, 26, 48, 107, 189

Ki-67 Antigen,Proliferating Cell Ki88(M) 15, 26, 48, 107, 189

p105 Proliferation-Associated Nuclear Antigen 2B3(M) 16, 49, 118,

Proliferating Cell Nuclear Antigen (PCNA) PC10(M) 16, 27, 49, 125, 190

PROSTATE MARKERS

Androgen Receptor F39.4.1(M) 11, 23, 43, 53, 184

Bcl-2 E17(R) 11, 23, 43, 54, 184


Bcl-x E18(R) 11, 23, 43, 55, 184



Cytokeratin 5 & Cytokeratin 14 EP24/EP61(R) 13, 25, 46, 85

Cytokeratin 14 LL002(M) 14, 25, 46, 87, 187


34ßE12/C51/AE1(M) 14, 46, 90, 187




Mucin 2 (MUC2) CCP58(M) 15, 48, 113

P504S (AMACR) 13H4(R) 16, 26, 49, 120, 190

P504S (AMACR) RBT-AMACR(R) 16, 26, 49, 120, 190

p63 4A4(M) 16, 27, 49, 121, 190

PAP A40010(M) 16, 27, 49, 121, 190

PIN4 Cocktail (P504S+HMW Cytokeratin+p63)

13H4(R)+34ßE12( R)+4A4(M) 16, 27, 147, 190

Prostate Specific Acid Phosphatase (PSAP) B01-94-21M-NA(M) 16, 27, 50, 125, 190

Prostate Specific Antigen(PSA) ErPr-8(M) 16, 27, 50, 126, 190

PSMA EP192(R) 17, 27, 50, 126, 190

RENAL CELL CARCINOMA MARKERS


RCC (Renal Cell Carcinoma) RCC-26(M) 17, 27, 50, 126, 190

SALIVARY DUCT CARCINOMA

GCDFP-15 EP1582Y(R) 14, 25, 47, 98, 188

Mucin 2 (MUC2) CCP58(M) 15, 48, 113

SEX CORD STROMAL TUMORS

Calretinin 2E7(M) 11, 23, 43, 60, 184


CD99 EPR3097Y(R) 13, 24, 45, 78, 186

Inhibin-Alpha R1(M) 15, 26, 48, 105, 189

SIGNAL TRANSDUCTION PROTEIN

Beta-Catenin E247(R) 11, 23, 43, 56 ,184

Catenin (p120) YE372(R) 16, 26, 44, 61, 185

CD3 epsilon EP449E(R) 12, 23, 44, 62, 185

CD5 4C7(M) 12, 23, 44, 64, 185


CD5 EP2952(R) 12, 23, 44, 64, 185

CD11C EP1347Y(R) 12, 24, 44, 66, 185

c-erbB-2 (HER-2/neu) CB11(M) 13, 24, 45, 81

c-erbB-2 (HER-2/neu) EP3(R) 13, 24, 45, 81

c-erbB-3 (HER-3) RTJ1/A2(M) 13, 24, 45, 81


EGFR (PAN) EP38Y(R) 14, 25, 47, 93

ErbB-2/HER-2 EP1045Y(R) 13, 24, 47, 95

Platelet-Derived Growth Factor (PDGF) PDGF88(M) 16, 49, 123

Platelet-Derived Growth Factor (PDGF) Polyclonal(R) 16, 49, 124

Stat 5-Alpha (C-term) E289(R) 17, 27, 50, 128,191

ZAP-70 ZAP70-C3(M) 17, 27, 50, 135, 191

ZAP-70 YE291(R) 17, 27, 50, 135, 191

SKELETAL MUSCLE

Desmin 33(M) 14, 28, 46, 92,187



Myoglobin MG-1(M) 16, 49, 115, 189


Myosin,Skeletal Muscle MY-32(M) 16, 49, 115, 189

p34cdc2 (Cyclin Dependent Kinase) POH-1(M) 16, 49, 119, 190

Sarcomeric Actin ZMSA-5(M) 17, 27, 50, 128, 190

Vimentin V9(M) 17, 27, 50, 134, 191

Vimentin,Non-Hematopoietic LN6(M) 17, 50, 134, 191

SKIN (ADNEXAL TUMORS)


CD15 (Blood group antigen Lewis X) BRA4F1(M) 12, 24, 44, 67, 185



SKIN (SPINDLE CELL TUMORS)

CD31 (PECAM-1) 9G11(M) 12, 44, 70, 185



Cytokeratins 8 & 18 5D3(M) 14, 25, 46, 87, 187

Factor VIII-Related antigen F8 2.2.9(M) 14, 25, 47, 97, 188

Factor XIII Subunit A E980.1(M) 14, 25, 47, 97, 188

Factor XIII A EP3372(R) 14, 25, 47, 97, 188

Glut-1 SPM498(M) 15, 25, 47, 100, 188


NGF Receptor EP31(R) 16, 26, 49, 117, 190

SMALL CELL CARCINOMA OF LUNG


SMALL, ROUND BLUE CELL TUMORS

Caldesmon E89(R) 11, 23, 43, 59, 184



CD61 EP2417Y(R) 13, 24, 45, 75, 186

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CD63 EP211(R) 13, 24, 45, 75, 186

CD68 KP1(M) 13, 24, 45, 76, 186

CD68 CD68/G2(M) 13, 24, 45, 76, 186

Myoglobin MG-1(M) 16, 49, 115, 189


PGP9.5 3D9(M) 16, 27, 49, 123

Vimentin V9(M) 17, 27, 50, 134, 191

SOFT TISSUE SARCOMA


CD99 EPR3097Y(R) 13, 24, 45, 78, 186

SOFT TISSUE TUMOR

Calretinin 2E7(M) 11, 23, 43, 60, 184


Desmin 33(M) 14, 28, 46, 92,187

SPINDLE CELL TUMORS

Beta-Catenin E247(R) 11, 23, 43, 56 ,184



Desmin 33(M) 14, 28, 46, 92,187

PGP9.5 3D9(M) 16, 27, 49, 123

STRESS RESPONSE PROTEIN

Heat Shock Protein (HSP-70) BRM-22(M) 15, 47, 101, 188

Heat Shock Protein 27 (HSP 27) G3.1(M) 13, 25, 47, 101, 188

SWEAT GLAND CARCINOMA

GCDFP-15 EP1582Y(R) 14, 25, 47, 98, 188

THYROID MARKERS





p21/WAF1 4D10(M) 16, 49, 119, 190

p27 (Kip1) DCS72(M) 16, 49, 119

p27 (Kip1) Y236(R) 16, 26, 49, 119, 190

Thyroglobulin 2H11(M) 17, 27, 50, 130, 191

Thyroid Stimulating Hormone (TSH) 5404(M) 17, 27, 50, 131

Thyroid Stimulating Hormone (TSH) Polyclonal(R) 17, 50, 131

Thyroid Transcription Factor (TTF-1) 8G7G3/1(M) 17, 27, 50, 131, 191

Thyroxine D5(M) 17, 50, 131, 191


TRANSITIONAL CELL CARCINOMA


TUMOR SUPPRESSORS, APOPTOSIS PROTEINS & ONCOPROTEINS

Bax Protein Polyclonal(R) 11, 43, 54

Bcl-2 E17(R) 11, 23, 43, 54, 184

Bcl-x E18(R) 11, 23, 43, 55, 184

BRCA1 Protein Polyclonal(R) 11, 43, 55

CD117 T595(M) 13, 24, 45, 79, 186


CD117 YR145(R) 13, 45, 79, 186

c-myc Protein 9E 10(M) 13. 45, 83, 187

Mucin 1 (MUC1) EPR1023(R) 15, 26, 48, 112, 189


NGF Receptor EP31(R) 16, 26, 49, 117, 190

p53 EP9(R) 16, 26, 49, 120, 190

p53 Protein BP53-12-1(M) 16, 26, 49, 120, 190

p53 Protein DO7(M) 16, 27, 49, 121, 190

p53 Protein 1801(M) 16, 27, 49, 121, 190

p63 4A4(M) 16, 27, 49, 121, 190

p63+CK5 Cocktail 4A4(M)+ EPR1600Y( R) 16, 27, 147

PDCD4 EPR3432(R) 16, 27, 49, 122, 190

UROTHELIAL CARCINOMA

S100P EP186(R) 17, 27, 50, 127, 190

VASCULAR ENDOTHELIAL

Aberrant Endothelial Cell 4A11(M) 11, 43, 51

bFGF (Basic Fibroblast Growth Factor) bFGF88(M) 11, 43, 57

VEGF Polyclonal(R) 17, 27, 50, 134, 191

WILM'S TUMOR


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