molecular tools: molecular separations *gel electrophoresis

Molecular Tools: Molecular Separations *Gel Electrophoresis

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Molecular Tools:

Molecular Separations

*Gel Electrophoresis

Molecular Tools:

Molecular Separations*Gel Electrophoresis

*One- and Two-Dimensional Gel Electrophoresis of Proteins

Molecular Tools:

Molecular Separations

*Gel Filtration Chromatography

Molecular Tools:

Labeled Tracers

*Autoradiography

*Phosphorimaging

Molecular Tools:

Nonradioactive Tracers

Molecular Tools:

Nucleic Acid Hybridization

* Southern Blots

H E BH E B H E BH E B H E BH E B H E BH E B

P1P1 P2aP2a P2bP2b P3P3

23.123.19.459.456.566.564.354.35

2.322.322.022.02

0.560.56

kbkb

Molecular Tools:

Nucleic Acid Hybridization

*DNA Fingerprinting/Typing

Molecular Tools:

Nucleic Acid Hybridization

*In Situ Hybridization

Glycogen phosphorylase gene

Minisatellite Telomeres

Sykorova, et al. (2006) Am. J. Bot. 93(6) 814-823

Page 9: Molecular Tools: Molecular Separations *Gel Electrophoresis

SDS-PAGESDS-PAGE

Isolate RibosomesIsolate Ribosomes

P3P3

S6S6

Incubate Incubate with with

AntiseraAntisera

Transfer to nitrocellulose YY

Molecular Tools:

Immunoblots (Western Blots)

Molecular Tools:

Sanger dideoxy DNA sequencing

Molecular Tools:Pyrosequencing (454 sequencing)

Molecular Tools:

Site-Directed Mutagenesis

Molecular Tools:

Northern Blots

P1P1

P2aP2a

P2bP2b

P3P3

18 S18 SrRNArRNA

Ear

Lea

eaf

Col

e opt

ile

Col

eopt

ile

Roo

oot

Silk

Pol

len

Pol

len

15 20 2515 20 25 15 20 2515 20 25 15 20 2515 20 25

bryo

Ale

uron

leur

one

dosp

erm

dosp

erm

DPPDPP

Page 14: Molecular Tools: Molecular Separations *Gel Electrophoresis

Fig. 5.34

Molecular Tools:Quantitative PCR concepts

Page 15: Molecular Tools: Molecular Separations *Gel Electrophoresis

Molecular Tools:Quantitative PCR quantification

Molecular Tools:

Reporter Gene Transcription

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molecular tools: molecular separations *gel electrophoresis

Documents

molecular separations

mutagenesis slide

phosphorimaging slide

gel electrophoresis

dna fingerprintingtyping

nucleic acid hybridization

northern blots p1 p2a

western blots