monica epifanio's urinomics lisbon 27th september 2015
TRANSCRIPT
Nanostructured Surfaces:
Development of a New MS-Platform to Capture,
Process and Analyse (CPA-MALDI)
Urine-Derived Biomolecular Compounds by
Monica Epifanio ([email protected])
Marie Curie fellow at Tethis S.p.A (Milan, Italy) and Dublin City University (Dublin, Ireland)
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SHOTGUN PRESENTATION
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To develop a new diagnostic technology based on:
Smart Nano-structured surfaces;
Suitable for MALDI -MS;
Micro-fluidics.
Aim of my work
Key challenges in proteomics-based clinical diagnostics
Continuous need and wish for higher sensitivity
Ability to analyse complex samples
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UNWASHED WASHED
UNWASHED WASHED ID=2 mm
6 microliters
Sample concentration and capturing
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NS-COATED PLATE
MSI Mass Spectrometry Imaging
VI. Data processing
III. MALDI analysis
V. Image reconstruction
II. Transfer on ITO+TiO2 slide and matrix deposition I. Sacrifice and organ dissection
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http://www.tethis-lab.com/
PEPTIDES PROTEINS
Trypsin digestion
CPA-MALDI
2D
Capturing-Processing-Analysing
1D
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Acknowledgment Dr. Emanuele Barborini Dr. Marc Bauman Dr. Alexander Yavorskyy Mr. Simone Vinati
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Mr. Giacomo Bertolini