monitoring and evolution of field gumboro disease pressure after … · monitoring and evolution of...

Monitoring and evolution of field Gumboro disease pressure after continuos vaccination with an Immune-Complex Gumboro disease

vaccine

CEVA POULTRY VACCINOLOGIST SUMMIT

Pablo Catalá Gregori

DVM, PhD, Dipl. ECPVS

CECAV – [email protected]

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2014 2

1. Introduction

2. Objective

3. Material and Methods

4. Results

5. Geographical Monitoring Tool

2014 3

1. Introduction

2. Objective


4. Results


Introduction

2014 4

1. Introduction

CECAV

IBD status SPAIN

In ovo vaccination

Descriptive results

Monitoring serological response

Detect IBVD strain

Alternative vaccination

Introduction

2014 5

1. Introduction

CECAV

IBD status SPAIN

In ovo vaccination

Descriptive results


Detect IBVD strain


Introduction

2014 6

CECAV: POULTRY QUALITY AND ANIMAL NUTRITION CENTER OF COMUNIDAD VALENCIANA

Non-profit, private and public service

Laboratory (100.000) + training (1.000)

Meat, Egg, Feed, Slautherhouse

Dep: Microbiology, Serology, Chemical, Other

CEVA collaboration

62nd Western Poultry Disease Conference - USA

Introduction

2014 7

1. Introduction

CECAV

IBD status SPAIN

In ovo vaccination

Descriptive results


Detect IBVD strain


Introduction

2014 8

1. Introduction

CECAV

IBD status SPAIN

In ovo vaccination

Descriptive results


Detect IBVD strain


Introduction

2014 9

IBD status in SPAIN (CRESA)

New Genotype since 2008

+ endemic vvIBD

Introduction

2014 10


New Genotype since 2008

+ endemic vvIBD

*CESAC:

2012 (27): 1 vvIBD

2013 (32): 9 vvIBD (7 broiler; 2 layer)

Introduction

2014 11


Endemic vvIBD

New Genotype:

Unspecific symptomatology

Macro: Bursa atrophy

Micro: Lymphocyte B loss

Introduction

2014 12

1. Introduction

CECAV

IBD status SPAIN

In ovo vaccination

Descriptive results


Detect IBVD strain


Introduction

2014 13

1. Introduction

CECAV

IBD status SPAIN

In ovo vaccination

Descriptive results


Detect IBVD strain


Introduction

2014 14

In ovo vaccination

Vaccines

Conventional (water) Live attenuated

Vector-based (in ovo; SC)

Immune-complex (in ovo; SC)

IBDV

2014 15

1. Introduction

2. Objective


4. Results


2014 16

1. Introduction

2. Objective


4. Results


OBJECTIVE

2014 17

1. Monitor serological response

2. Detect IBDV strain

• After In ovo single vaccination with Immune-complexvaccine

• After Alternative

In ovo single vaccination with Immune-complexvaccine / conventional live vaccination

In ovo single vaccination with Immune-complexvaccine / vector based

2014 18

1. Introduction

2. Objective


4. Results


2014 19

1. Introduction

2. Objective


4. Results


Material and Methods

2014 20

• >5 years (2009-2014)

• Spain

• >3.500 Broiler Flocks; 8/15 serum per flock; >35.000 samples

• In ovo single vaccination Cevac TRANSMUNE® d18

• Sampling ≥26d (at farm or slaughterhouse)

Serum – ELISA (Biochek) (SERAVIAN + CECAV)

Random Bursa – RT- PCR+Sequencing; >200 samples; (CRESA + CESAC)

2014 21

1. Introduction

2. Objective


4. Results


2014 22

1. Introduction

2. Objective


4. Results


Results

2014 23

R² = 0,9242

0

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29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53

Me

an E

LISA

GM

TIT

ER

Age (Days)

Fig. 1. ELISA GM titers in Vaccinated Broilers with an Immune-Complex IBD Vaccine

(2009 - 2014; 3.781 flocks)

2009-2014

Trend (Polynomial)

Results

2014 24

R² = 0,9242

0

1000

2000

3000

4000

5000

6000

7000

8000

29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53

Me

an E

LISA

GM

TIT

ER

Age (Days)

Fig. 1. ELISA GM titers in Vaccinated Broilers with an Immune-Complex IBD Vaccine

(2009 - 2014; 3.781 flocks)

2009-2014

Trend (Polynomial)

0

20

40

60

80

100

120

140

160

180

200

29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 D

Year 2009

Year 2010

Year 2011

Year 2012

Year 2013

Year 2014*

Fig. 2. Serological Coefficient of Variation in Vaccinated Broilers with an Immune -Complex IBD Vaccine (2009 - 2014)

Days of Age

Co

eff

icie

nt

of

Var

iati

on

(%

)Results

2014 25

≥2012; ≥40d;

Results

2014 26

≥2012; ≥40d;

0

20

40

60

80

100

120

140

2012 2013 2014

CU-1WT

GM 97

vvIBD

D 78

CH 80

No secuenciable

Atenuada

W 2512

Results

2014 27

Fig. 3. Bursa IBDV strain sequence results (2012 - 2014)

W&TRM 76,2%

N=109 N=116 N=18*

W&TRM 77,8%*W&TRM 81,0%

W 84,4% W 81,0% W 88,9%


2014 28

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IBD titer & %CV(Mar - Nov 2012; 78 flocks)

Título Medio %CV

Cambio pauta vac IBD

Immunocomplex Live


2014 29

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IBD titer & %CV(Jul - Sept 2012; 30 flocks Apr - Dec 2013; 16 flocks)

Título Medio %CV

Cambio pauta vac IBD

Immunocomplex Live


2014 30

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IBD titer & %CV(Mar - May 2012; 157 flocks)(Jan - May 2013; 97 flocks)

(Mar - Apr 2014; 112 flocks)

Título Medio %CV

Cambio pautavac IBD

Immunocomplex Vector - based

Results

2014 31

1. Serological response

GM titer: Rising trend as the broiler grow (stab. 6,000&34d)

Single vaccination – uniform flock immunization

%CV: Downward trend over the years (under 40% since 2012 from 40d)

Improvement of the proper hatchery vaccination

2. IBDV strain

Winterfield 2512

Vaccine presence >75% & TRM

3. Alternative vaccination

Impaired %CV

2014 32

1. Introduction

1. Broiler production EU-SPAIN

2. IBD status SPAIN

3. In ovo vaccination

2. Objective


4. Results


2014 33

1. Introduction

1. Broiler production EU-SPAIN

2. IBD status SPAIN

3. In ovo vaccination

2. Objective


4. Results


Geographical Monitoring Tool

2014 34

Geographical Monitoring IBDV response to single vaccination:

Farm Location

New 2012 “real time” tool (day, week, month, year,…)

Regular automatic reports in mailbox

ELISA biochek; ELISA idexx

Fully Configurable Mapping Tool


2014 35

2012 IBD Broilers >35d


2014 36

2013 IBD Broilers >35d


2014 37

2014* IBD Broilers >35d


2014 38

OIE Mapping Tool

http://www.oie.int/wahis_2/public/wahid.php/Diseaseinformation/Diseasedistributionmap

6 months; Month period; country

Example: IBD, January to June 2013

http://www.oie.int/wahis_2/public/wahid.php/Diseaseinformation/Diseasedistributionmap


2014 39

BIOPORTAL Mapping Tool

http://bioportal.ucdavis.edu/

Example: LPAI; Europe



2014 40

BIOPORTAL Mapping Tool


Example: LPAI; Europe; Spain


Thank you

CEVA POULTRY VACCINOLOGIST SUMMIT

Pablo Catalá Gregori

DVM, PhD, Dipl. ECPVS

CECAV - [email protected]
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