‘monitoring of individuals - human health campus · pdf filebrief history 2007.3.27 ......
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Research Project entitled
‘Monitoring of Individuals
following Acute and Chronic Exposures
to Ionizing Radiation’
(IAEA CRP E3.50.08 project: 17085/R0)
Chang-Mo Kang, Ph.D.
KIRAMS
(Korea Institute of Radiological & Medical Sciences)
2013. 6. 13
Korea Institute of
Radiological & Medical Sciences
(KIRAMS)
1963.12.17 Launched as Radiation Medicine Research Institute
1973.2.7 Reorganized as Korea Cancer Center Hospital (KCCH)
1997.8.2 Introduced PET first in Korea
2002.6.4 National Radiation Emergency Medical Center
2002.7.19 Operated CyberKnife first in Korea
2002.10.11 Developed Cyclotron(13MeV) first in Korea
2010.7.16 Opened DIRAMS in Busan
Brief History
2007.3.27 Reorganized as KIRAMS
1986.11.17 Operated Cyclotron(50MeV) first in Korea
Organization
Radiological & Medical
Research Institute
DIRAMS (Busan Branch)
Korea Cancer
Center Hospital
National Radiation
Emergency Medical Center
KIRAMS
Korea Heavy Ion Med
Accelerator Project
Head Office of Management & Planning
Workforce : Total 1,198 (as of 2012)
2012 Budget : USD 219 million
Workforce & Budget
Korea Cancer
Center Hospital National Radiation
Emergency Medical Center
Korea Heavy Ion
Medical Accelerator Project
Korea Institute of Radiological
& Medical Sciences
Radiological & Medical
Research Institute
Division of Radiation
Cancer Research
Radiation sensitive technique
Radiation manifold technique
Medical physics treatment technique for high accurate radiotherapy
Division of Molecular
Imaging Research
Disease-specific radiopharmaceuticals
In vivo applications for target-specific molecular imaging & therapy
Imaging-based in vivo quantitative method for preclinical & clinical study
Division of Clinical &
Translational Research
Translational research on cancer diagnosis & treatment
Biomarker, new drug candidates
Cancer patients database for research
Division of Radiation
Effect
Radiation effect by low and high dose irradiation
Biodosimetry with the use of cytogenetics
Functional recovery of impaired body system using tissue engineering
Center of Radiation
Treatment Research
Toxicity evaluation of new agents for radiation treatment
Radiation irradiation to SPF animal for validation test
DIVISIONS
- 6 -
The 1st cancer specialized hospital in Korea (1963)
24 clinical departments and 495 beds
First-in-Korea history
- Color Scanner (1967)
- Gamma Camera (1969)
- Cyclotron (1986)
- CyberKnife (2002)
- PET/CT (2003)
- PET-MRI system for whole body (2009)
Development of Heavy Ion Radiotherapy system (USD 183 million, 2010~16)
Development of therapeutic radiopharmaceuticals (USD 85 million, 2013~16)
Cooperation with
IAEA & WHO
Education & Training
Research &
Development
National Radiation
Emergency Medical
Preparedness
9
Title : Korea Heavy Ion Medical Accelerator Development Project (KHIMA)
- Development of the first heavy ion therapy system in Korea based on
the experience on Cyclotron development at KIRAMS
Leading organization : Korea Institute of Radiological & Medical Sciences
Location : Kijang County, Busan Metropolitan City (adjacent to DIRAMS)
Project Period : 2010 ~ 2016
Project Budget : USD 183 million (195 billion KRW)
Funding : KIRAMS, MEST, Busan Metropolitan City, Kijang County
Total Area : 88,139m2
Located in Pusan (2nd largest city of Korea)
Opened in July, 2010
- Cancer Hospital (304 beds)
- Research Center
10
Research Project entitled
‘Monitoring of Individuals
following Acute and Chronic Exposures
to Ionizing Radiation’
(IAEA CRP E3.50.08 project: 17085/R0)
Detailed CRP work plan (2012. 2 ~ 2016. 3)
.
Establishment of calibration curves for 4 different biodoimetry
techniques in Korean Network System:
- Dicentric chromosome (DC) assay
- Fluorescent In Situ Hybridization (FISH): Ch #1, 4, 8
- Micronuclei in binuclei (MNBN) assay
- Premature Chromosome Condensation (PCC) assay
and
- Creation of a network of laboratories across Korea
with the capability of providing radiation dose estimates
(0, 0.1, 0.25, 0.5, 0.75, 1, 2, 4, 6Gy)
• A revised International Atomic Energy Agency (IAEA) manual
was published in 2001 and 2011.
IAEA Revised Manual
EPR-BIODOSIMETRY 2011, IAEA TECHNICAL REPORTS SERIES no. 405, 2001, IAEA
from EPR-BIODOSIMETRY 2011, IAEA
Representative Cytogenetic Assays
Chemical PCC
Assay
Interphase-based
Bioassays
Metaphase-based
Bioassays
PCC Assay
(Mitotic-Cell Fusion)
Micronuclei
Assay
from EPR-BIODOSIMETRY 2011, IAEA
Cytogenetical Assays for Dose Assessment
following Exposure to Ionising Radiation
1. Dicentric Chromosome (DC) assay
2. Micronuclei in binucleated cells (MNBN)
4. Premature Condensed Chromosome (PCC)
3. Translocations using
Fluorescence in situ hybridization (FISH)
0-3 / 1000
2-30 / 1000 BNC*
0 / 1000
Spontaneous frequency Biological end-points
* In both micronucleus and FISH based translocation assays,
spontaneous frequency was found to be age dependent.
3-16 / 1000*
Detailed CRP work plan (2012. 2 ~ 2016. 3)
.
Establishment of calibration curves for 4 different biodoimetry
techniques:
- Dicentric chromosome (DC) assay
- Fluorescent In Situ Hybridization (FISH): Ch #1, 4, 8
- Micronuclei in binuclei (MNBN) assay
- Premature Chromosome Condensation (PCC) assay
and
- Creation of a network of laboratories across Korea
with the capability of providing radiation dose estimates
Dicentric chromosome (DC) assay
Ring
Ring
Dicentric
Dicentric
Dicentric
Tric.
Tric.
Korean Standard Curve (KIRAMS)
Dose
(Gy)
Number of
scored
cells
Number of
chromosome
with DC
Frequency
0.00 5,000 9 0.002
0.10 5,000 14 0.003
0.25 5,000 41 0.008
0.50 5,000 81 0.016
0.75 5,000 168 0.034
1.00 5,000 285 0.057
2.00 3,989 886 0.222
Korean DC Curve
y = 0.0548x 2 + 0.0001x + 0.0027
R 2 = 0.9998
0.00
0.05
0.10
0.15
0.20
0.25
0.00 0.50 1.00 1.50 2.00
Dos e (Gy )
Fre
que
ncy
y = 0.0024 + 0.0160D + 0.1001D2 (+/- 0.0010 ) (+/- 0.0079 ) (+/- 0.0066 )
(dose rate: 0.5Gy/min)
DoseEstimate_ver 4.1 Created by Liz Ainsbury
by Dose estimation program for biodosimetry
Detailed CRP work plan (2012. 2 ~ 2016. 3)
.
Establishment of calibration curves for 4 different biodoimetry
techniques:
- Dicentric chromosome (DC) assay
- Fluorescent In Situ Hybridization (FISH): Ch #1, 4, 8
- Micronuclei in binuclei (MNBN) assay
- Premature Chromosome Condensation (PCC) assay
and
- Creation of a network of laboratories across Korea
with the capability of providing radiation dose estimates
Fluorescent In Situ Hybridization (FISH)
Ch #1, 2, 4 (all green)
Ch # 1, 19 (red) / 4, 18 (green)
from EPR-BIODOSIMETRY 2011, IAEA
Detailed CRP work plan (2012. 2 ~ 2016. 3)
.
Establishment of calibration curves for 4 different biodoimetry
techniques:
- Dicentric chromosome (DC) assay
- Fluorescent In Situ Hybridization (FISH): Ch #1, 4, 8
- Micronuclei in binuclei (MNBN) assay
- Premature Chromosome Condensation (PCC) assay
and
- Creation of a network of laboratories across Korea
with the capability of providing radiation dose estimates
MN assay in binucleated cells
Cytochalasin B
Giemsa stained
from EPR-BIODOSIMETRY 2011, IAEA
MN assay in binucleated cells
Acridine orange stained
A
centromere negative
B
centromere positive
MN assay with pancentromeric probe
Detailed CRP work plan (2012. 2 ~ 2016. 3)
.
Establishment of calibration curves for 4 different biodoimetry
techniques:
- Dicentric chromosome (DC) assay
- Fluorescent In Situ Hybridization (FISH): Ch #1, 4, 8
- Micronuclei in binuclei (MNBN) assay
- Premature Chromosome Condensation (PCC) assay
and
- Creation of a network of laboratories across Korea
with the capability of providing radiation dose estimates
R-PCC.(Chemical induced PCC)
Cells are stained with Giemsa (left)
and hybridized with human alpha
satellite DNA probe according to
FISH painting (right). Chemical
methods of inducing PCC, using
inhibitors of DNA phosphorylation,
such as calyculin A or okadaic acid
have also been developed.
Premature Chromosome Condensation
FICOL SEPARATION FUSE IN PEG
LYMPHOCYTES CHO
CHINESE HAMSTER
OVARY (CHO) CELLS
COLCEMID
MITOTIC SHAKE OFF
(METAPHASE CELLS)
Fusion-PCC Incubation for 1 h
(Medium+Colcemid)
PERIPHERAL BLOOD
from EPR-BIODOSIMETRY 2011, IAEA
Detailed CRP work plan (2012. 2 ~ 2016. 3)
.
Establishment of calibration curves for 4 different biodoimetry
techniques:
- Dicentric chromosome (DC) assay
- Fluorescent In Situ Hybridization (FISH): Ch #1, 4, 8
- Micronuclei in binuclei (MNBN) assay
- Premature Chromosome Condensation (PCC) assay
and
- Creation of a network of laboratories across Korea
with the capability of providing radiation dose estimates
and harmonize
REAC/TS
Presentation of Gordon Livingston (REAC/TS)
KIRAMS #17
Standard Technique
to make the samples
and
to read the samples
are
very important.
New Korean Biodosimetry Network Laboratories
A
C
B
D
A: KIRAMS(Korea Institute of Radiological & Medical Sciences)
y = 0.0024 + 0.0160D + 0.1001D2 (dose rate: 0.5Gy/min)
(+/- 0.0010 ) (+/- 0.0079 ) (+/- 0.0066 )
B: RHRI (Radiation Health Research Institute)
y = 0.00042 + 0.04484D + 0.03778D2 (dose rate: 0.8Gy/min)
C: KFDA (Korea Food and Drug Administration)
D: DIRAMS
Web-based reading the captured
slide photos for network among
KIRAMS(Korea Institute of
Radiological & Medical
Sciences),
RHRI (Radiation Health
Research Institute)
and KFDA (Korea Food
and Drug Administration)
2003: Workshop was opened for a standard technology transfer to national
biodosimetrist in KIRAMS.
2004. 11. 4 - 12: IAEA-supported National Workshop was opened for a standard
technology transfer to Korean biodosimetrist with IAEA specialists whose Dr.
Hayata and Dr. Darroudi in KIRAMS.
2005: Workshop was opened for a standard technology transfer to national
biodosimetrist in KIRAMS.
2006: Workshop was opened for a standard technology transfer to national
biodosimetrist in KIRAMS.
Biodosimetry workshop for a standard technology transfer in KIRAMS
From NAME
BANGLADESH Mr. Md. Mustafizur RAHMAN
CHINA Mr. Zhijun GUO
INDONESIA Ms. Yanti LUSIYANTI
IRAN, ISLAMIC REPUBLIC OF Mr. Seyed Abolghasem HAERI
KOREA, REPUBLIC OF Mr. Jung-Eun LEE
LEBANON Mr. Salim CHAMMAS
MALAYSIA Ms. Noraisyah MOHD YUSOF
PAKISTAN Mr. Fazal-e- RAHIM
PHILIPPINES Ms. Juana GREGORIO
QATAR Ms. Leena AL-SULAITI
SRI LANKA Ms. Hiranya WILLIAMS
SYRIAN ARAB REPUBLIC Mr. Khaled ALMOHAMAD
THAILAND Ms. Darunee PEEKHUNTHOD
VIETNAM Mr. Que TRAN
YEMEN Ms. Sawsan ANAAM
2007. 4. 30 - 5. 4: IAEA-supported Regional Workshop was opened for a standard
technology transfer to 15 Asian/Pacific biodosimetrist with IAEA specialists
whose Dr. Blackly, Dr. Darroudi and Dr. Zombori in KIRAMS.
Programme: I.A.E.A. Ref. C7-RAS-0.050-001
Title: Regional Training Course on Biological Dosimetry
Place: Korea Institute of Radiological and Medical Sciences, Seoul, Republic of Korea
Periods: April 30 ~ May 4, 2007
2007. 4. 30 - 5. 4: IAEA-supported Regional Workshop in KIRAMS
International Network for Radiation Emergency Medical Preparedness
International Network for Population Triage after Multi-Casualties
Biological Dosimetry
at KIRAMS
(Korea) Biological
Dosimetry
in Leiden Univ.
(The Netherlands)
Biological
Dosimetry
in AFRRI
(U.S.A.)
Biological
Dosimetry
in REACT/TC
(U.S.A.)
Biological
Dosimetry
in CMANA
(China)
Biological
Dosimetry
in NIRS
(Japan)
MOU MOU
MOU ROD
Biological
Dosimetry
in RAMS
(Russia)
MOU plan MOU plan
Thank you
for your attention!