VITO NV | Boeretang 200 | 2400 MOL | Belgium | Tel. + 32 14 33 55 11 | www.vito.be

VITO supports healthcare innovations and performs toxicity screening An Van Rompay, Hilda Witters, Rita Cortvrindt, Lieve Geerts and Reinhilde Weltens VITO NV, Boeretang 200, 2400 Mol, Belgium

ENVIRONMENT HEALTH & SAFETY

Toxicology services and hands-on support REACH & CLP Have no secrets to us Toxicology testing services We perform Medical devices We take care of biocompatibility Label We apply Validation Studies We are your partner Safety by design We support the future

How can we help? Our multi-disciplinary teams have the expertise to accomplish all your toxicology and regulatory needs. Data collection, compound analysis, fill the gaps (QSAR’s or read across), perform testing services (ecotoxicity, biodegradation studies, in vitro human toxicity), validation studies, exposure scenarios, safety analysis, second opinion,… full dossier preparation and follow up. GLP certified testing facility EU-NETVAL registered lab

Skin irritation » SkinEthicTM RHE, EpidermTM* (OECD 439) Eye irritation » BCOP (OECD 437), BCOP – LLBO » SkinEthicTM HCE » ICE (OECD 438)*, HET-CAM* » EpiOcularTM EIT* (draft OECD) » STE* (draft OECD) Skin corrosion » Corrositex® (OECD 435) » SkinEthicTM RHE, Epiderm* (OECD 431) Skin sensitization » VITOSENS®

» DPRA* (OECD 442C) » KeratinoSens* (OECD 442D) Phototoxicity » 3T3 NRU* (OECD 432), EpiDerm™* Cytotoxicity (ISO 10993) » Cell lines (HepG2, 3T3, …) » Endpoints: NR-uptake, MTT,… Inflammation responses » Quantification of cytokines Genotoxicity » Ames II / MPF / Penta I Embryo - & Developmental neurotoxicity » Zebrafish embryo & larvae (up to age of 5 days) Validation studies

Genomics » qPCR » Microarrays Nano SafetyO

Endocrine disruptionO

In vitro toxicity & biocompatibility tests

* Under implementation O On request

Ecotoxicity & biodegradation tests

Computational models & expert analysis Read across and QSAR » Physicochemistry: SPARC, EPI-Suite » OECD (Q)SAR Toolbox » Environment: VEGA, BIOWIN, ECOSAR, CASE Ultra, TOPKAT » Human endpoints: ToxTree, VEGA, TTC Exposure modelling » Consumers: ConsExpo, AISE REACT, EN-forc » Environment: EUSES » Workers: ECETOC-TRA, Stoffenmanager, ART, EMKG-EXPO, Riskofderm » S-Risk, EN-forc and other expert models Risk assessment Socio-economic analysis Product classification » DETNET expert

Algae growth inhibition test » Pseudokirchneriella subcapitata, OECD 201 Lemna growth inhibition test » Lemna major, OECD 221 Daphnia immobility test » Daphnia magna, OECD 202 Daphnia reproduction test » Daphnia magna, OECD 211 Fish acute toxicity, and prolonged toxicity test » Danio rerio & Oncorhynchus mykiss, OECD 203

– OECD 204 Fish embryo acute toxicity test (FET) » Danio rerio, OECD 236 Fish, short-term toxicity test on Embryo and Sac-fry stages » Danio rerio, OECD 212 Worm mortality test » Eisenia fetida, OECD 207 Worm reproduction test » Eisenia fetida, OECD 222 Plant growth inhibition test » Several species, OECD 208 Biodegradation tests » OECD 301B, OECD 301D, OECD 302 Contact An Van Rompay Tel. +32 14 33 52 46 an.vanrompay@vito.be Rita Cortvrindt Tel. +32 477 277 006 rita.cortvrindt@vito,be