Outline of TalkGlobal perspectiveNeed of Hour: MSC as a drug moleculeDrugs ≠ Regeneration thus Stem CellsWhat is Regenerative MedicineStem Cells are the main streamMSC- as drug

India

Innovations are required…new treatment therapies are required………..

“We can’t solve the problem by using the same kind of thinking we used when we created them”

- Albert Einstein

A Paradigm Shift in HealthcareHeading towards cure from signs and symptoms

suppressionAssure complete safety and minimum side effectsAlso ensure long-term benefits

Need of the hour….

“Cells are not single molecular agents but are rather, site-regulated, adaptive agents capable of very complex functions involving a multitude of bioactive factors and reactivities.”

MSC: A complete genome for healing is available

Drug: A single molecule with limited applications

Repair

Stem Cell Potential..RepairRejuvenateReplaceRestoreMaintainEnhance…. This is regardless of any cause for the injury

to the cell or tissue or organ….

Stem Cells Applications : Hype or Hope

“ Imagine if a doctor says to you that you are diagnosed with an untreatable condition and you have to live with it and die with it ….what will be your state !!!!!”

A story from fiction to facts

“I have thought a lot about stem cells , and I am sure that when the historians will look back at the 20th century…the greatest scientific achievement will not be from the field of space technology or computer technology but will be from the tissue engineering and genetic medicine.”

-Michael Goulian-ABC Scientific News Reporter,

29-9-1999.

Are we churning the old wheel again??

YOU ARE ALIVE….

Blood Every Second

Skin Every 2 Weeks

Bones Every 20 Years

Your Innate Regenerative Capacity

Regenerative Medicine

Tissue Engineering

MSCs and PericytesChang’s hypothesisPericytes are present on the capillaries and micro-

vesselsThese pericytes appear to be getting recruited by

body’s intelligence upon any injury…Pericytes also appear to understand the injury

process as well as the required repair mechanism…

They are the mechanics, getting all the spare parts in place..or may be working just as bio-catalysts

All MSCs are pricytes…but all pericytes are not MSCs….

MSC as antibacterial activity, antibacterial protein

Human CAP-18/LL37

This calls for a new thought… Are MSCs merely MSCs or…they actually are..“Medicinal Signaling Cells” …?

A complete regenerative micro-environment created…Act like an injury-specific drug store!!

Trophic Factors

Over-all drug-like actions of MSCsImmuno-modulatory: control immune function in

facvour of bodyAnti-inflammatory: reduce unwanted

compensatory mechanismsAnti-apoptotic: Allow regenerationCell differentiation: all functions regainedProtein and cytokine synthesis (through mRNA

activation): Micro-environment enrichmentAngiogenic: assurance of continued effectAnti-Bacterial activity & Release micro verticals

transporting functional mRNA & micro RNA

One MSC….ALL ACTIONS!!

What is MSC exactly???Mesenchymal Stem Cell??

Mesenchymal Stromal Cell??

Medicinal Signaling Cell??

ISCT definitionA cell that adheres to plasticExpresses CD 105, 90, 73Does not express CD 34, 45 and 13HLA DR is absent

All these virtues of this MSC make them interesting, innovative drug molecule…

Additional features…MSCs can be customized as per need…

The immunomodulating action can be modified as per need…

TLR 4 priming…. Pro-inflammatory---MSC1TLR3 priming….immuno-suppressive…MSC2

How about tumorogenicity??Autologous MSCs Allogenic MSCsDNA garbage

accumulationMutationsSenescenceTelomer e shorteningMalignancy not possible

Xeno in nature..Hence, preferential

elimination by the recipient body

No scope for any malignancy

This very fact makes Allogenic-MSCs as best choice future drugs!!

“ You carry your own repairing kits in your body”