ms_final seminar-hao
TRANSCRIPT
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Hao Wu
Supervisors: Dr. Appel , Dr. Hu
Committee member: Dr. Peng Zeng
July 8, 2013
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Overview
Introduction: Cockroach, the Dubia Cockroaches
Part I: Instar determination
Part II: Parental care
Part III: Temperature-dependent development
Question?
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1 Locomotion: Ground, Water and Air
2 Diets: Omnivores (or unknown)3 Reproduction: Great diversity Oviparity A, Oviparity B Ovoviviparity A, Ovoviviparity B Viviparity4 Sociality: Solitary, Subsocial, Eusocial (termites)5 Relation to Humans: Pests, Pet
Diet, Pet, Medicine
Cockroaches (Blattodea)
The Classical Cockroach Phylogeny of McKittrick (1964)
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The Dubia Cockroaches, Blaptica dubia(Blaberidae)
Locomotion: Ground
Diets: Omnivores
Reproduction:
Ovoviviparity A
Sociality:
Subsocial (Parental Care)
Relation to Humans:
Pet, Pet Diet
Source: http://en.wikipedia.org/wiki/File:Blaptica-
Dubia-cockroaches-x3-male-female-juvenile-v2.jpg
Oothec a of the Dubia Cockroaches, Photo
courtesy of Charles Stephen
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The Dubia Cockroaches as food for pets
Excellent, protein-rich diet for insect eating pets
Various lizards, turtles, and snakes
Spiders
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Overview
Introduction: Cockroach, the Dubia Cockroaches
Part I: Instar determination
Part II: Parental care
Part III: Temperature-dependent development
Question?
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Part I Instar determination using Gaussian mixture model Basic research and application Growth, evolution
Life table analysis
Brooks-Dyar rule (Brooks 1886, Dyar 1890, Hutchinson 1984)
Objective:
Find a general way to determine instars of B. duia
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MethodsStatistical analysis
Assumption: The measurement of sclerotized characters in each instar is Gaussian
distribution
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Methods
Data collection
1925 cockroaches were measured
Measure the three variables: head width, pronotum length and width of the Dubia cockroach rearing in the lab (30℃, 12:12 (L:D) and supplied with water and dry dog Chow)
Statistical analysis
Overview
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MethodsStatistical analysis
Gaussian Mixture model (GMM) (Johnson & Wichern 2007)
Mixture of two Gaussian
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Gaussian Mixture model (GMM)
Three variables: head Width, Pronotum Length and Pronotum Width.
The multivariate case
More than two groups
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GMM
Inference is based on maximum likelihood
(Johnson & Wichern 2007)
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LikelihoodWe do not know the group the data belong to. i.e. We
do not know which instar the insect is, when we take the measurements
Hierarchical clustering, EM algorithms and Bayesian Information Criterion (BIC)
Software : R package mclust (Fraley & Adrian 2012)
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Results
Table 1. The 95% simultaneous -intervals of the three characters (mm) in each instar
Instar 1 Instar 2 Instar 3 Instar 4 Instar 5 Instar 6 Instar 7
N 137 152 148 234 356 324 178
Length [2.37,2.45] [2.91,3.03] [3.77,3.87] [4.84,4.98] [6.11,6.23] [7.46,7.63] [9.30,9.47]
Width [4.14,4.22] [5.13,5.30] [6.40,6.54] [7.89,8.13] [9.86,10.04] [12.15,12.41] [15.04,15.30]
Head [1.78,1.80] [2.06,2.11] [2.47,2.51] [2.94,3.00] [3.53,3.58] [4.21,4.28] [5.00,5.07]
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Result assessment
Table 2. The mean of the three characters(mm) in each instar and growth ratio
Characters Instar 1 Instar 2 Instar 3 Instar 4 Instar 5 Instar 6 Instar 7 Ratio
Length 2.41 2.97 3.82 4.91 6.17 7.54 9.39 1.26 Width 4.18 5.21 6.47 8.01 9.95 12.28 15.17 1.24
Head 1.79 2.08 2.49 2.97 3.55 4.24 5.04 1.19
Brooks-Dyar rule
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In another study, the development of B. dubia was monitored at the same
conditions as stated above [30 ± 2°C,12:12 (L:D) h]. We directly observed seven
instars in B. dubia over a period of 6 months.(although there is few exception). In
addition, Hintze-Podufal and Nierling (1986) also indicated that there are seven
instars in B. dubia reared at 28 ± 2°C.
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Part I Summary
This approach can be used to determinate instars of the Dubia cockroaches.
1. The growth rates follow Brooks-Dyar rule.
2. There are 7 instars in the Dubia cockroaches Blaptica dubia.
3. The growth rates of pronotum length, pronotum width and head width are 1.26, 1.24, 1.19 respectively.
This approach should be applicable to other insects, since the Dubia cockroaches comply with the same growth model with other insects
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Overview
Introduction: Cockroach, the Dubia Cockroaches
Part I: Instar determination
Part II: Parental care
Part III: Temperature-dependent development
Question?
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Part II Parental care effect
Objective: Determine if parental care affects development of the Dubia Cockroaches
Hypothesis: The presence of parents affects development of the Dubia Cockroaches
Method:
Compare the difference of
the head width, the pronotum
width and length, and the weight
difference in the last instar.
Compare the difference of the development time
Data Analysis:
ANOVA
Diagram of Experiment Design
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Effect of parental care on development time
Table 3 Effect of parental care on development time (days)
Group number Neonate only With Adult female With adult male P value
1 142.9±9.662 139.7±11.715 141.4±9.985 0.810
2 165.3±6.873 147.0±13.476 158.1±17.201 0.083
3 202.5±8.536 210.4±34.122 222.5±28.224 0.330
4 167.6±12.293 161.5±19.206 160.3±11.056 0.590
5 130.4±9.812 130.8±9.203 132.9±7.120 0.830
6 175.7±25.050 171.8±15.106 158.8±25.246 0.280
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Table 4 Effect of parental care on head width (mm)
Group number Neonate only With Adult female With adult male P value
1 5.079±0.1451 5.062±0.1737 5.033±0.2315 0.89
2 5.409±0.1384 5.367±0.1370 5.444±0.0967 0.49
3 5.233±0.1175 5.215±0.1428 5.244±0.0760 0.87
Group number Neonate only With Adult female With adult male P value
1 9.693±0.2693 9.732±0.3798 9.631±0.4897 0.89
2 10.10±0.3466 10.25±0.2518 10.07±0.2362 0.40
3 9.933±0.3678 9.787±0.3298 9.876±0.2452 0.62
Table 5 Effect of parental care on pronotal length (mm)
Effect of parental care on measurable characters
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Table 6 Effect of parental care on pronotal width (mm)
Group number Neonate only With Adult female With adult male P value
1 15.22±0.2531 15.17±0.6773 15.37±0.6217 0.77
2 16.25±0.3955 16.05±0.3808 15.87±0.7114 0.33
3 15.49±0.5815 15.70±0.3813 15.63±0.4670 0.66
Group number Neonate only With Adult female With adult male P value
1 2.070±0.2062 2.122±0.2615 2.046±0.2952 0.86
2 2.644±0.1862 2.743±0.1750 2.487±0.2739 0.076
3 2.057±0.2271 2.165±0.2291 2.163±0.2734 0.57
Table 7 Effect of parental care on body weight (g)
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Part II summary No parental care effect is detected.
Application:
No need to put an adult when start new colony.
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Overview
Introduction: Cockroach, the Dubia Cockroaches
Part I: Instar determination
Part II: Parental care
Part III: Temperature-dependent development
Question?
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Part IITemperature-dependent development
Objective: Find the ideal
temperature range for the
development of the Dubia
cockroaches
Hypothesis: 30°C~35°C will be
the best temperature range
Method: degree days
Data Analysis:
Linear regression
Diagram of Experiment Design
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Degree-Days Calculation
Development time (Days , Hours)
Development rate( 1/D)
Temperatue(T)
1/D = kT + b
1/D = k(T –T0)
( T0 is the development threshold)
Degree days = D(T-T0) = 1/k
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Table. 8 Development time of each instar in different temperature. (mean (SD) n)
instar 20°C 25°C 30°C 35°C
1 38.8 (3.96) 28 25.4(2.84) 36 20.08(3.23) 38 17.46(5.19) 59
2 79.9(5.931) 28 51.18(3.79) 34 36.06(4.63) 36 NA
3 116.15(8.60) 26 78.32(4.83) 31 53.26(7.28) 34 NA
4 161.74(13.91) 27 106.76(7.10) 33 70.28(8.41) 36 NA
5 220.48(24.54) 27 133.45(7.71) 33 87.56(8.91) 36 NA
6 NA 167.80(10.48) 105.70(17.00) NA
7 NA 233.20(17.55) 149.20(15.69) NA
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Figure 2 The linear regression of development time of 1st instar
in 20°C, 25°C, 30°C, 35°C with 95% confidence interval.
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Figure 3 The linear regression of development time of 2nd instar in
20°C, 25°C, 30°C with 95% confidence interval.
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Table 3. Linear regression models of temperature-dependent development in the seven instars of B. dubia
Instar Model R squred F P ( > |t|)Lower Development
Threshold (°C)Degree days
1st instar Y = 0.0021X – 0.0148 0.984 120 < 0.01 7.02 457.5
2nd instar Y = 0.0149X – 0.0172 0.997 301 0.0366 11.56 668
3rd instar Y = 0.001023X – 0.01203 0.989 90.8 0.0666 11.83 1031
4th instar Y = 0.000805X – 0.010190 0.986 69 0.0763 12.66 1317
5th instar Y = 0.000689X – 0.009398 0.993 151 0.0157 13.65 1515
6th instar Y = 0.0007X – 0.0117 NA NA NA 16.48 1429
7th instar Y = 0.000483X – 0.007783 NA NA NA 16.12 2071
Summay
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Acknowledgement Dr. Arthur Appel
Dr. Xing Ping Hu
Dr. Peng Zeng
Marla Eva
Charles Stephen
Zachary Devries
Znar Barwary
Yao Xu
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Question? Thank you