mubasher, m phil synoses seminar
TRANSCRIPT
M Phil Synopsis Seminar
Effect of dietary selenium supplementation on body score and growth performance of male and female goats
Under Kind Supervision of
Dr. Mool Chand MalhiAssistant Professor
Department of Veterinary Physiology & Biochemistry
ByNabi B. Solangi
M Phil Scholar
SELENIUM
IntroductionAbsorption and metabolismFunctionsSelenoproteinsDeficienciesEffect on growth performance
(Krzyzewski et al., 2014).
Selenium | Introduction
Selenium is an essential trace element for maintenance of normal
physiological process
Anti-inflammatory responses
Immunological
Endocrinological
Antioxidative
Selenium found in organic and inorganic forms, mainly inorganic form is used in animal diets
(e.g. Sodium Selenite or selanate).
(Yue et al., 2009).
Selenium | Introduction
Selenium found in organic and inorganic forms, mainly inorganic form is used in animal diets (e.g. Sodium Selenite or selanate).
However in ruminants the absorption of inorganic selenium is much lower.
Ruminants = 34% (sheep), Non ruminants = 85% (swine)
(Yue et al., 2009).
Selenium | Introduction
Absorption Metabolism
Sodium Selenite Se+4
Selenite Radical Se+4
Selenide Se-2 Excretion Urine, sweat etc
Passive diffusionRelease of free radical
Selenomethionine in SY Se-2
Selenomethionine Se-2
Active Transportation
Selenocystine Se-2
Selenocystine in SY Se-2
Selenoprotein/ Body Proteins muscle, milk, egg etc
GSH-Px,SeP, SeW
+ Cysteine
Active Transport, Direct use
Absorption and Metabolism
Selenoproteins | GPX activityGlutathione peroxidase (GPXs),Thioredoxin reductase (TRs),Iodothyronine deiodinase (TRs),
as well as two selenoproteins
Selenoprotein P (SeP) andSelenoproteins W (SeW).
(J. Krzyzewski et al., 2014; Ahmed et al., 2016)).
• Mentioned together with GSH• The enzyme (GPXs) found in liver, kidney, lungs, pancreas, spleen, skeletal muscle and GIT.
• Protects cell from oxidative damage
• Integral part of antioxidation system• Prevent toxic effects of oxidants, e.g. Hydrogen per oxide (produced as a natural product during oxygen metabolism)
Functions• Prevent peroxidation• Immunity • Neuro-physiological function• Fertility• Maintenance of pregnancy• Act of parturition • Neonatal growth.
(J. Krzyzewski et al., 2014)
Selenium Deficiency• Deficiency of selenium can lead to miscarriages, early
embryonic death and spermatogenesis disorders in bulls.
And it also causes…….• Infertility• Loss of pregnancy (early embryonic death)• Retention of placenta• Neonatal weakness• Clinical symptoms of WMD (usually observed in kids).
(Ramirez-Bribiesca et al., 2005; Krzyzewski et al., 2014).
(Yue et al., 2009).
Growth Performance
Selenium
Growth
Biosynthesis of selenoproteins
Reproduction
Health
Growth performance
ed protein and energy intake
ed carcass fat + Muscle mass
internal fat
Goats fed HC diet out on more fat on GIT Goats usually deposit
Important
More
Colomer-Rocher et al., 1992; Kirton 1988
Less
Sub cutanious+intramuscular
Hango et al., 2007
PRESENT STUDY
HypothesisObjectivesBrief methodology andImplications of study.
Hypothesis
We hypothesized that increasing Se level in HC-diet
May increase tissue Seconc., and thus GSH-Px, which may ultimately reduce the oxidativestress.
HC + Se supplemented goats get better growthrate thus body weight gainincreases as compareto the control group and HC diet.
Selenium
ObjectivesThe object of the present study is to compare effect of HC, LC,
HCS and LCS diets on:
• Growth performance• Physiological variables• And to evaluate economics
of selenium supplemented male and female goats (no.=20).
Brief Methodology
• Experimental Animals: 20 young cross bred female goats (4 mo, 12-13 kg BW)
• Place of study: Livestock Experimental Station, Department of Livestock, Faculty of AHVS, SAU Tando Jam, Pak.
• Adaptation Period: 2 weeks• Experimental design: Five groups
LC (F)= Low concentrate diet, concentrate: Roughage ratio (35:65)HC (F)= High concentrate diet, concentrate: Roughage ratio (65:35)HCS (F)= HC diet supplemented with selenium yeast (SY) @ 0.3 mg Se/kg. diet.
LC (M)= Low concentrate diet, concentrate: Roughage ratio (35:65) LCS (M)= HC diet supplemented with selenium yeast (SY) @ 0.3 mg Se/kg. diet.
• Experimental period: 10 Weeks
Brief Methodology
Adaptation Experimental Period
Weeks
Slaughtering, Sampling, Measurements and Analyses
Slaughtering
Carcass characterstics
Feed intake= feed offered – refusal
Parameters | Growth performance
BCS BW FCR
Initial and final BCS
Lumber regionRibsSternum
Average BW on weekly basis
Average daily gain (ADG)Dry matter intake (DMI)Gain efficiency of each goat
FCR =ADG/DMI
Wt. gain = F W – I W
FCR
BCS 1 to 5
Parameters
Physiological variables Economics
Body TemperaturePulse rateRespiratory rate
Per KG expenditure will be calculated to analyze:
Expenditure Implementation of the research Feasibility report
Implications of the study
The research we are going to conduct will be helpful to….
• Improve production (i.e. milk, meat, calving interval)• Formulate balanced diet• Get rid of deficiency syndromes• Make feasibility report for goat farming
