muscle tissue -nsu

8/14/2019 Muscle Tissue -NSU

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Histology 6

Craig Clifford

Northeastern State University

Spring 2009


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Muscle Tissue

Responsible for movement in organs

and the body as a whole

There are three types of muscle in the

body (mesodermal origin) Skeletal

Cardiac Smooth


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Skeletal muscle

Very long cylindrical, multinucleated

cells

Quick, forceful, voluntary contractions

Cross-striations


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Cardiac muscle

Elongated, branched uninucleate cells

Cross-striations

Cells joined at intercalated disks

Involuntary, vigorous rhythmic

contractions


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Smooth muscle

Short, spindle shaped, uninucleate

cells

Slow involuntary contractions


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Special terms

In muscle cells, The cytoplasm is referred to as the

sarcoplasm The cell membrane is called the

sarcolemma

The smooth ER is know as thesarcoplasmic reticulum


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Skeletal muscle

Muscle fibers=muscle cells Very long and may be as long as the

muscle itself Multinucleated state results from the

fusion of mononucleated cells

embryonically Nuclei located peripherally, which differs

from cardiac and smooth


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Skeletal muscle

Muscle fibers contain myofibrils

Each muscle cell is surrounded by an

endomysium A bundle of muscle cells is called a fascicle

and is surrounded by a perimysium

A muscle is composed of multiple fasciclesand is surrounded by the epimysium


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Stained for laminin

NERVE

Laminin is a protein component of the endomysium.


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Tissue culture plasticware

coated without Laminin

NG-108 rat

glioma/mouse

neuroblastomacells cultured on

tissue-culture

plastic are loosely

adhered andremain rounded.


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Tissue culture plasticware

coated with Laminin

NG-108 rat

glioma/mouse

neuroblastoma cells

cultured on BDBioCoat Laminin

Cellware exhibit a

spindle-shaped

morphology anddendritic processes.

stimulate neurite outgrowth, promote cell attachment, chemotaxis,

cell differentiation and neuronal survival.


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Skeletal muscle

Note the peripheral

location of the

nuclei


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Skeletal muscle

When viewed with light transmitted

through the sections, a banding

pattern appears. A bands

I bands

Z lines H zones


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Rigor mortis

Stiffness of the body beginning 3-4hours post-mortem, peaking at 12

hours and relenting at 36 hours. Contrast:

Pallor mortis no blood flow, immediate Algor mortis decrease in temp.

1.5 F/hour

Livor mortis blood pooling (lividity) Precedes Rigor mortis


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Proprioception - Muscle

spindles


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Muscle spindles - xs

Artifact?


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Proprioception Golgi Tendon

Organ


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Proprioception Golgi Tendon

Organ


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Camillo Golgi

Born 1843

Died 1926

Physician trained inItaly

Initial studies

focused on the

nervous system


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Myoglobin stain

From what structure in the

body is this section taken?

Immunohistologically stained

for myoglobin


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Cardiac muscle

Remember, typically uninucleate (may

be binucleate), branching,

interconnecting cells.


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Cardiac muscle

The internal

arrangement of

subcellularcomponents is not

as orderly in

cardiac as opposed

to skeletal muscle.


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Cardiac muscle

Intercalated disks are areas of

connection between cells, containing

Fasciae adherentes (hemi-Z bands) Maculae adherentes (desmosomes)

Gap junctions


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Smooth muscle

Smooth muscle cells are elongated,

non-striated cells

Fusiform in shape - larger in middleand narrow at each end.

No t-tubules


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muscle tissue -nsu

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