nanoquot operator's manual · 2014-04-14 · contacting biotek instruments, inc. | vii...

Operator’s Manual

Microplate Dispenser

NanoQuot™

NanoQuot TM

Microplate Dispenser Operator's Manual

December 2006 © 2006 Part Number 7151000 Revision A BioTek® Instruments, Inc.

ii | Preface

BioTek Instruments, Inc.

Notices

BioTek® Instruments, Inc.

Highland Park, P.O. Box 998

Winooski, Vermont 05404-0998 USA

All Rights Reserved

© 2006, BioTek® Instruments, Incorporated. No part of this publication may be reproduced, transcribed, or transmitted in any form, or by any means electronic or mechanical, including photocopying and recording, for any purpose other than the purchaser’s use without written permission of BioTek Instruments, Inc.

Trademarks

BioTek® is a registered trademark, and NanoQuot™, NanoQuot™ PC Control Software, and Bio-Stack™ are trademarks of BioTek Instruments, Inc.

Microsoft®, Windows®, and the Windows logo are registered trademarks of Microsoft Corporation in the United States and other countries. All other trademarks are the property of their respective holders.

Restrictions and Liabilities

Information in this document is subject to change, and does not represent a commitment by BioTek® Instruments, Inc. Changes made to the information in this document will be incorporated in new editions of the publication. No responsibility is assumed by BioTek for the use or reliability of software or equipment that is not supplied by BioTek or its affiliated dealers.

Contents | iii

NanoQuot Operator’s Manual

Contents

Preface...........................................................................................i

Notices............................................................................................... ii All Rights Reserved.......................................................................... ii Trademarks.................................................................................... ii Restrictions and Liabilities ................................................................ ii

Contacting BioTek Instruments, Inc.......................................................vii Customer Service and Sales.............................................................vii Service/TAC ..................................................................................vii European Coordination Center/Authorized European Representative ......vii

Document Conventions....................................................................... viii About This Manual......................................................................... viii

Revision History ................................................................................. ix Intended Use Statement ....................................................................... x Quality Control .................................................................................... x Repackaging and Shipping .................................................................... x Warnings............................................................................................ x Hazards and Precautions...................................................................... xi

Hazards ........................................................................................ xi Precautions ...................................................................................xii

CE Mark Information .......................................................................... xiii EC Directive 73/23/EEC Low Voltage (Safety).................................... xiii EMC Directive 89/336/EEC Electromagnetic Compatibility.................... xiii Directive 2002/96/EC Waste Electrical and Electronic Equipment.......... xiii

Electromagnetic Interference and Susceptibility .....................................xiv USA FCC CLASS A .........................................................................xiv Canadian Department of Communications Class A..............................xiv

User Safety........................................................................................xv North America ...............................................................................xv International .................................................................................xv

Safety Symbols .................................................................................xvi

Chapter 1: Introduction.................................................................1

Introducing the NanoQuot™ .................................................................. 2 Dispense Head................................................................................ 2 Software ........................................................................................ 3 Liquid Profiles ................................................................................. 4 Compatibility with the Bio-Stack™ ..................................................... 5

Hardware Features............................................................................... 6

iv | Preface


Package Contents ................................................................................ 6 Optional Accessories & Replacement Parts............................................... 7 Specifications...................................................................................... 8

Microplates..................................................................................... 8 Hardware and Environmental ............................................................ 8 Electrical........................................................................................ 8 Dispense Accuracy and Precision ....................................................... 8 PC (Optional).................................................................................. 9

Product Support & Service .................................................................. 10 Contacting the Technical Assistance Center (“TAC”) ........................... 10 Returning Instruments for Service/Repair ......................................... 10

Chapter 2: Installation ................................................................11

1: Unpack and Inspect the Instrument.................................................. 12 2: Select an Appropriate Location ........................................................ 13 3: Remove Shipping Hardware ............................................................ 13 4: Install the NanoQuot’s Components.................................................. 16

Connect the Tubes and Bottles ........................................................ 16 Connect the Power Supply .............................................................. 22 (Optional) Connect the Serial Cable ................................................. 22 (Optional) Connect the Programmer’s Cable...................................... 23 (Optional) Install Software ............................................................. 24

5: Turn on the NanoQuot and Verify Performance................................... 26 Self-Test ...................................................................................... 26 Fluidics System Test ...................................................................... 26

Installation of the Bio-Stack with the NanoQuot ..................................... 27 Repackaging and Shipping .................................................................. 28

Chapter 3: Operation ...................................................................31

Overview.......................................................................................... 32 Manual Control vs. PC Control ......................................................... 32

NanoQuot User Interface .................................................................... 33 Rocker Switches............................................................................ 33 Thumbwheels ............................................................................... 33 Keypad Functions and LEDs ............................................................ 34

General Usage Guidelines ................................................................... 34 Before Running Any Program .......................................................... 34 Maintenance................................................................................. 36 Programming Considerations .......................................................... 36 Adjust the Dispense Head............................................................... 36

Define A1 Locations ........................................................................... 37 Manual Control of the NanoQuot™........................................................ 38

Purge .......................................................................................... 38

Contents | v


Prime .......................................................................................... 39 Dispense...................................................................................... 40 Step ............................................................................................ 41

PC Control of the NanoQuot™.............................................................. 42 Purge .......................................................................................... 43 Prime .......................................................................................... 43 Clean .......................................................................................... 44 Dispense Programs........................................................................ 45 Liquid Profiles ............................................................................... 47

Chapter 4: Instrument Qualification............................................53

Overview.......................................................................................... 54 IQ/OQ/PQ......................................................................................... 54 Qualification Schedule ........................................................................ 56 Dispense Accuracy and Precision Tests.................................................. 57

Specifications ............................................................................... 57 Required Materials......................................................................... 58 Test Solution Recipes..................................................................... 59 Equipment Setup .......................................................................... 59 Dispense Accuracy Test Procedure ................................................... 60 Dispense Precision Test Procedure ................................................... 62 Dispense Accuracy & Precision Worksheet: 0.10 µl Dispense................ 63 Dispense Accuracy & Precision Worksheet: 1.0 µl Dispense ................. 64

Chapter 5: Preventive Maintenance.............................................65

Recommended Maintenance Schedule................................................... 66 Overview ..................................................................................... 66 Schedule...................................................................................... 67

Warnings and Precautions ................................................................... 68 Tools and Supplies ............................................................................. 69 Rinse the Fluid Path ........................................................................... 70

Purge and Prime at the Start of the Day ........................................... 70 Clean at the End of the Day ............................................................ 71 Remove Protein Residuals and Fungi Growth ..................................... 71

Clean Components............................................................................. 72 Clean the Bottles and Tubes ........................................................... 72 Clean the Fluidics Cover ................................................................. 72 Clean the Dispense Head................................................................ 73 Clean the Plate Carrier System........................................................ 75 Clean the Priming Trough ............................................................... 76 Clean the Compressor.................................................................... 76

Decontamination ............................................................................... 77 Decontaminate External Surfaces .................................................... 78

vi | Preface


Decontaminate the Fluid Path ......................................................... 79 Replace Components.......................................................................... 80

Replace a Solenoid Valve Assembly.................................................. 80 Replace the Fluid Path Assembly ..................................................... 84

Prepare for Storage or Shipment.......................................................... 86 Remove Liquid from the Fluid Path................................................... 86 Store the Dispenser in an Appropriate Environment............................ 86

Maintenance Logs .............................................................................. 87

Chapter 6: Troubleshooting .........................................................91

Overview.......................................................................................... 92 Troubleshooting Charts....................................................................... 93

Dispenser Start-Up........................................................................ 93 Fluid Delivery ............................................................................... 93 Fluid Leakage ............................................................................... 95 Microplate Carrier Movement .......................................................... 96 Dispense Head Movement .............................................................. 96 PC-to-Dispenser Communication ..................................................... 96

Appendix A: Error Codes..............................................................97

Overview.......................................................................................... 98 Errors During Operation with the Bio-Stack ........................................... 99 NanoQuot™ Instrument Errors............................................................100 PC Software Errors............................................................................102

Appendix B: Chemical Compatibility ..........................................105

Components and Materials Composition ...............................................106

Contacting BioTek Instruments, Inc. | vii


Contacting BioTek Instruments, Inc.

BioTek® Instruments, Inc.

Highland Park, P.O. Box 998 Winooski, Vermont 05404-0998 USA

Customer Service and Sales

Internet: www.biotek.com

Phone: 888-451-5171 (toll free in the U.S.)

802-655-4740 (outside the U.S.)

Fax: 802-655-7941

E-Mail: [email protected]

Service/TAC

Phone: 800-242-4685 (toll free in the U.S.)

802-655-4740 (outside the U.S.)

Fax: 802-655-3399


European Coordination Center/Authorized European Representative

BioTek® Instruments GmbH

Kocherwaldstrasse 34 D-74177 Bad Friedrichshall Germany

Internet: www.biotek.de

Phone: +49 (0) 7136 9680

Fax: +49 (0) 7136 968 111


mailto:[email protected]

viii | Preface


Document Conventions

This manual uses the following typographic conventions:

Example Description

This icon calls attention to important safety notes.

Warning! A Warning indicates the potential for bodily harm and tells you how to avoid the problem.

Caution A Caution indicates potential damage to the instrument and tells you how to avoid the problem.

Note: Bold text is primarily used for emphasis.

This icon calls attention to important information.

About This Manual

The intent of this Operator’s Manual is to instruct the new user how to set up and operate BioTek’s NanoQuot™ Microplate Dispenser. To help you read and understand this manual, certain document conventions have been used.

Major topic headings start a new page (such as Document Conventions, above) to give you a visual and style clue that a new major subject is being introduced. One or more subheadings may appear below each major heading.

Revision History | ix


Revision History

Revision Date Changes

A 12/06 Initial release.

x | Preface


Intended Use Statement

• The NanoQuot™ Microplate Dispenser provides for dispensing to 96- , 384-, and 1536-well microplates in flat, round, and “V” bottom configuration.

• This product may only be used for Research and Development and other nonclinical uses.

Quality Control

It is considered good laboratory practice to run laboratory samples according to instructions and specific recommendations included in the assay package insert for the test to be conducted. Failure to conduct Quality Control checks could result in erroneous test data.

Repackaging and Shipping

Important! Save all packaging materials. If you need to ship the NanoQuot to BioTek for service or repair, you must use the original packing. Other forms of commercially available packing are not recommended and can void the warranty. See Repackaging and Shipping in Chapter 2, Installation, for complete repackaging instructions.

Warnings

Operate the NanoQuot™ Microplate Dispenser on a level surface and away from excessive humidity.

When operated in a safe environment, according to the instructions in this manual, there are no known hazards associated with the NanoQuot. However, the operator should be aware of certain situations that could result in serious injury.

Do not reach into the instrument during operation; some areas of the NanoQuot can present pinch hazards when the unit is operating.

Hazards and Precautions | xi


Hazards and Precautions

Hazards

Warning! Power Rating. The NanoQuot Dispenser’s power supply must be connected to a power receptacle that provides voltage and current within the specified rating for the system. Use of an incompatible power receptacle may produce electrical shock and fire hazards.

Warning! Electrical Grounding. Never use a plug adapter to connect primary power to the instrument. Use of an adapter disconnects the utility ground, creating a severe shock hazard. Always connect the system power cord directly to an appropriate receptacle with a functional ground.

Warning! Internal Voltage. Always turn off the power switch and unplug the power cord before cleaning the Dispenser’s outer surface.

Warning! Liquids. Avoid spilling liquids on the Dispenser; fluid seepage into internal components creates a potential shock hazard. Wipe up all spills immediately. Do not operate the instrument if internal components have been exposed to fluid.

Warning! Potential Biohazards. Some assays or specimens may pose a biohazard. Adequate safety precautions should be taken as outlined in the assay’s package insert. Always wear safety glasses and appropriate protective equipment, such as chemically resistant rubber gloves and apron.

Warning! Pressure Hazard. Do not exceed recommended pressures of 30 psi inlet pressure and 15 psi to the 100-ml glass supply bottle! Pressures outside the specified limits may cause the bottle to burst and result in possible injury.

Warning! Aerosol Hazard. Do not let air follow fluid into the supply tubing during an operation. This will cause the fluid to be emitted as an aerosol. Depending on the fluid, this could be hazardous to your health.

Warning! Unspecified Use. Failure to operate this equipment according to the guidelines and safeguards specified in this manual could result in a hazardous condition.

xii | Preface


Precautions

The following precautions are provided to help avoid damage to the instrument:

Caution: Service. Authorized BioTek service personnel should service the Dispenser. Only qualified technical personnel should perform troubleshooting and service procedures on internal components.

Caution: Environmental Conditions. Do not expose the instrument to temperature extremes. For proper operation, ambient temperatures should remain between 10º to 40ºC (50° to 104°F). Performance may be adversely affected if temperatures fluctuate above or below this range. Storage temperature limits are broader.

Caution: Static Sensitive. Do not expose the instrument to electrical discharges on any conductive surfaces.

Caution: Power Supply. Only use the correct line voltage when operating the Dispenser.

Caution: Chemical Compatibility. Some chemicals may cause irreparable damage to this instrument. The following chemicals have been deemed safe for use in this instrument: buffer solutions (such as PBS), saline, surfactants, deionized water, 70% ethyl, isopropyl, or methyl alcohol, 40% formaldehyde, and 20% sodium hydroxide. Other chemicals may cause severe damage to the instrument. Contact BioTek Instruments if you require further assistance. Refer to Appendix B, Chemical Compatibility.

Caution: Sodium Hypochlorite. Do not expose any part of the instrument to sodium hypochlorite solution (bleach) for more than 20 minutes. Prolonged contact may damage the instrument surfaces. Be certain to rinse and thoroughly wipe all surfaces.

Caution: Buffer Solution. Although many precautions have been taken to ensure that the instrument is as corrosion-proof as possible, the Dispenser is not sealed and liquids can seep into sensitive components. Make sure that any spilled buffer solution is wiped off the Dispenser. Continuous exposure to salt solution may corrode parts of the microplate carrier, movement rail, springs, and other hardware.

Caution: Warranty. Failure to follow preventive maintenance protocols may void the NanoQuot™ warranty. See Chapter 5, Preventive Maintenance.

Caution: Disposal. This instrument contains printed circuit boards and wiring with lead solder. Dispose of the instrument according to Directive 2002/96/EC, “on waste electrical and electronic equipment (WEEE).”

Hazards and Precautions | xiii


Based on the testing described below and information contained herein, this instrument bears the CE mark

EC Directive 73/23/EEC Low Voltage (Safety)

IEC/EN 61010-1 (2001)

“Safety Requirements for Electrical Equipment for Measurement, Control and Laboratory Use, Part 1: General Requirements.”

EMC Directive 89/336/EEC Electromagnetic Compatibility

Emissions - CLASS A

IEC 61326-1:1997/ +A1 to A3

CISPR 11 & EN 55011 Class A

Immunity

IEC 61326-1:1997/ +A1 to A3

IEC 61000-4-2 Electrostatic Discharge

IEC 61000-4-3 Radiated EM Fields

IEC 61000-4-4 Electrical Fast Transient/Burst

IEC 61000-4-5 Surge Immunity

IEC 61000-4-6 Conducted Disturbances

IEC 61000-4-11 Voltage Dips, Short Interruptions and Variations

EN 61000-3-2 Harmonics Current Emission

EN 61000-3-3 Voltage Fluctuations and Flicker

Directive 2002/96/EC Waste Electrical and Electronic Equipment

Disposal Notice

This instrument contains printed circuit boards and wiring with lead solder. Dispose of the instrument according to Directive 2002/96/EC, “on waste electrical and electronic equipment (WEEE).”

xiv | Preface


Electromagnetic Interference and Susceptibility

USA FCC CLASS A

Warning: Changes or modifications to this unit not expressly approved by the manufacturer could void the user’s authority to operate the equipment.

This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the FCC Rules.

These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment. Like all similar equipment, this equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in accordance with the instruction manual, may cause harmful interference to radio communications. Operation of this equipment in a residential area is likely to cause interference, in which case the user will be required to correct the interference at their own expense.

Canadian Department of Communications Class A

This digital apparatus does not exceed Class A limits for radio emissions from digital apparatus set out in the Radio Interference Regulations of the Canadian Department of Communications.

Le present appareil numerique n’met pas du bruits radioelectriques depassant les limites applicables aux appareils numerique de la Class A prescrites dans le Reglement sur le brouillage radioelectrique edicte par le ministere des Communications du Canada.

User Safety | xv


User Safety

The NanoQuot™ Microplate Dispenser has been type tested by an independent laboratory and found to meet the requirements of the following:

North America

• Canadian Standards Association CAN/CSA C22.2 No. 61010-1-04


• Underwriter’s Laboratories UL 61010-1-2004


International

• See IEC/EN 61010-1 (2001) on page xiii.

xvi | Preface


Safety Symbols

Some of these symbols may appear on the instrument.

Only qualified personnel who recognize shock hazards and are familiar with the safety precautions should repair this instrument. Read the manual carefully before operating this instrument.

Alternating current Courant alternatif Wechselstrom Corriente alterna Corrente alternata

Direct current Courant continu Gleichstrom Corriente continua Corrente continua

Both direct and alternating current Courant continu et courant alternatif Gleich - und Wechselstrom Corriente continua y corriente alterna Corrente continua e corrente alternata

Earth ground terminal Borne de terre Erde (Betriebserde) Borne de tierra Terra (di funzionamento)

Protective conductor terminal Borne de terre de protection Schutzleiteranschluss Borne de tierra de protección Terra di protezione

On (Supply) Marche (alimentation) Ein (Verbindung mit dem Netz) Conectado Chiuso

Safety Symbols | xvii


Off (Supply) Arrêt (alimentation) Aus (Trennung vom Netz) Desconectado Aperto (sconnessione dalla rete di alimentazione)

Caution (refer to accompanying documents) Attention (voir documents d’accompanement) Achtung siehe Begleitpapiere Atención (vease los documentos incluidos) Attenzione, consultare la doc annessa

Warning, risk of electric shock Attention, risque de choc électrique Gefährliche elektrische Schlag Precaución, riesgo de sacudida eléctrica Attenzione, rischio di scossa elettrica

Warning, hot surface Attention, surface chaude Warnen, heiße Oberfläche Precaución, superficie caliente Attenzione, superficie calda

Warning, risk of crushing or pinching Attention, risque d’écrasement et pincement Warnen, Gefahr des Zerquetschens und Klemmen Precaución, riesgo del machacamiento y sejeción Attenzione, rischio di schiacciare ed intrappolarsi

Separate collection for electrical and electronic equipment Les équipements électriques et électroniques font l’objet d’une collecte sélective Getrennte Sammlung von Elektro- und Elektronikgeräten Recogida selectiva de aparatos eléctricos y electrónicos Raccolta separata delle apparecchiature elettriche ed elettroniche

xviii | Preface


Chapter 1

Introduction

This chapter introduces the NanoQuot™ Microplate Dispenser and describes its hardware and software features and technical specifications. Instructions on how to contact BioTek for Product Support & Service are included on page 10.

Introducing the NanoQuot™ ....................................................... 2 Dispense Head..................................................................... 2 Software ............................................................................. 3 Liquid Profiles ...................................................................... 4 Compatibility with the Bio-Stack™ .......................................... 5 Hardware Features.................................................................... 6 Package Contents ..................................................................... 6 Optional Accessories & Replacement Parts.................................... 7 Specifications........................................................................... 8 Microplates.......................................................................... 8 Hardware and Environmental ................................................. 8 Electrical............................................................................. 8 Dispense Accuracy and Precision ............................................ 8 PC (Optional)....................................................................... 9 Product Support & Service ........................................................10 Contacting the Technical Assistance Center (“TAC”) .................10 Returning Instruments for Service/Repair ...............................10

2 | Chapter 1: Introduction


Introducing the NanoQuot™

Compact, fast, and easy to use, the NanoQuot is a self-contained, user-programmable, universal microplate dispenser. As the best value in sub-microliter dispensers available today, the NanoQuot dispenses volumes down to 100 nl precisely and accurately into standard-height as well as shorter-profile microplates.

The following unique combination of features expands applications far beyond the typical microplate dispenser:

• The NanoQuot can be operated using just the keypad and thumbwheel input, or via a computer, under the control of the NanoQuot™ PC Control Software. For high throughput dispensing requirements, the NanoQuot can be operated with a Bio-Stack™ Microplate Stacker or other robotic systems.

• Precision solenoid valves and a pressurized dispense system ensure precise fluid delivery, as well as reproducibility for repeated dispenses.

• An 8-tip fluid system can dispense to 96-, 384-, and 1536-well plates. Flat, round, and “V” bottom geometries are supported.

• The NanoQuot has a wide dispense volume range of 100 nl to 40 μl per well, with aqueous solutions, and can dispense a variety of solutions, including buffered saline and reagent solutions. A dead volume < 2 ml ensures negligible reagent loss.

• An intuitive interface allows you to run a program by simply selecting the volume and specifying the number of columns. The NanoQuot will then process the plate according to the program parameters.

• The NanoQuot may be used for a wide variety of applications, including ELISA, cell-based assays, PCR setups, diluent dispensing, and compound storage.

• Several pre-defined functions are provided to simplify preventive maintenance, which should be performed regularly to ensure optimum Dispenser performance.

• The NanoQuot has an extremely small footprint, which saves precious bench space.

Dispense Head

The Dispense Head is the Dispenser component that delivers fluid to the microwells. Each NanoQuot™ is equipped with a dispense head containing 8 dispensing valves. The dispense head can dispense into 96-, 384-, and 1536-well microplates, and it can be easily removed for cleaning or replacement.

Introducing the NanoQuot™ | 3

NanoQuot Operator's Manual

Software

On-Board Software

The NanoQuot Dispenser is operated by on-board software, controlled through the Dispenser’s keypad, thumbwheels, and rocker switches. This on-board software, referred to as the “basecode,” has an identifying part number.

Your Dispenser is installed with NanoQuot basecode part number 7150201-FW. The basecode also has an identifying version number. If you need to contact BioTek Instruments for technical assistance, you will be asked for the part number and version of the basecode currently residing on your Dispenser. This information may be viewed in the NanoQuot™ PC Control Software’s main screen, in the Instrument Software field.

The 7150201-FW basecode supports the following features:

• Easy-to-use manual controls (keypad, thumbwheels, switches).

• Ability to define and run Dispense and Step programs for automated dispensing to microplates.

• Storage of up to three programmable internal liquid profiles. (See Liquid Profiles on page 4).

• Configurable program parameters, such as liquid profile selected, dispense volume, plate type, and ending column.

• Predefined maintenance programs Purge and Prime, which are used to remove all air from the fluid lines and keep the valves wet and free from clogging.

NanoQuot™ PC Control Software

The NanoQuot PC Control Software allows you to run Prime, Purge, Clean, and Dispense programs, as well as

• Create Liquid Profiles (LPs)

• Download LPs to the NanoQuot™

• Define the A1 location

• Specify starting and stopping columns

• Select tips used for dispensing

• Perform tip priming The NanoQuot PC Control Software is required for display of error codes and basecode software information, and for use of the 13-point liquid profiles developed and tested by BioTek for the NanoQuot. See the next section, Liquid Profiles.



Liquid Profiles

When dispensing sub-microliter volumes, numerous variables can affect accuracy. The NanoQuot™ uses an air compressor to force fluid through a valve to the wells of the microplate. The amount of fluid dispensed through the valves is regulated by a Liquid Profile. The Liquid Profile defines the length of time to keep open the valves to dispense the required volume. Liquid Profiles are fluid specific: the more viscous the fluid, the longer the valves must be kept open to dispense fluid. Conversely, at the same pressure, less viscous fluid can be dispensed in a shorter time period.

Liquid Profiles are also considered instrument-specific. They correct for any deviation in performance due to hardware variations in the dispenser and compressor.

Up to five Liquid Profiles can be stored on the dispenser. Two of these profiles are pre-defined and cannot be changed. The other three profiles are programmable and can be downloaded to the dispenser using the PC Control software. After the profiles are loaded on the dispenser, you can detach it from the PC and operate it manually.

BioTek installs several Liquid Profiles with the NanoQuot PC-Control Software. You should test these profiles with your NanoQuot. If you are not satisfied with the dispense performance (at varying pressure settings), create your own profiles for your dispenser. You must also create Liquid Profiles for fluids not defined by the installed profiles, including fluids with different concentrations (%) than the installed profiles.

The NanoQuot is shipped with these three Liquid Profiles loaded:

LP1: DI water, 12.5 PSI

LP2: DMSO 100%, 12.5 PSI

LP3: Glycerol 40%, 12.5 PSI

Chapter 3, Operation contains instructions for creating, modifying, and downloading Liquid Profiles, using the NanoQuot PC Control Software.

Introducing the NanoQuot™ | 5


Compatibility with the Bio-Stack™

The NanoQuot™ is compatible with BioTek’s Bio-Stack™ Microplate Stacker and Bio-Stack Twister II Microplate Handler.

Controlled serially by the NanoQuot, the Bio-Stack can rapidly and systematically transfer microplates one at a time to and from the Dispenser, and includes:

• Removable stacks (one input and one output) that can each hold up to 30 standard height or 45 half-height plates (e.g., low volume 384-well or 1536-well).

• Optional restacking of plates to maintain correct sequencing. If you have purchased the Bio-Stack to operate with the NanoQuot, see the Bio-Stack Operator’s Manual for instructions on configuring the Dispenser to run with the Bio-Stack. If you are interested in purchasing the Bio-Stack, contact your local BioTek dealer for more information.

Figure 1: The NanoQuot Dispenser with the Bio-Stack Microplate Stacker



Hardware Features

• Thumbwheel input

• Rocker switches

• Membrane keypad with five action keys

• Dispenses to 96-well microplates with 0.355” (9 mm) well centers

• Dispenses to 384-well microplates with 0.177” (4.5 mm) well centers

• Dispenses to 1536-well microplates with 0.885” (2.25 mm) well centers

• External 12 VDC power supply

• One serial RS-232 COM port (9-pin female connector)

• Controllable flow rates and volumes provided by high-speed solenoid valves. Settings are adjustable via the on-board software and air pressure regulator, for low- to high-velocity dispensing

• Stepper motors provide accurate and repeatable carrier and manifold positioning

• Dispenses to 96-, 384-, and 1536-well plates, standard and half-height depths

• Priming trough built into the chassis

Package Contents

• NanoQuot™ Microplate Dispenser

• External 12 VDC power supply with power cord (PN 61101)

• RS-232 serial cable, DB9F to DB9M (PN 75116)

• Programmer’s cable (PN 75117) and DB9F to RJ-12 adapter (PN 48660)

• Pressure gauge assembly (PN 76071), that includes regulator, gauge, and On/Off toggle switch, attached to a mounting plate

• 100 ml glass supply bottle with cap and tubing to distribution manifold (PN 48659)

• Fluid path assembly (PN 76070), that includes a dispense head, 8 solenoid valves, 8 tubes, distribution manifold, and supply tubing

• Waste tubing, 3/8” ID, cut to length 5’ (PN 71226)

• Air tubing, 1/8” ID, cut to length 10’ (PN 71229)

• 3/32” hex wrench (PN 98259)

Optional Accessories & Replacement Parts | 7


• 0.050” hex wrench (PN 98260)

• Compressor fitting, 115V (PN 48664)

• Compressor fitting, 230V (PN 48665)

• NanoQuot™ PC Control Software/Programmer’s Package CD (PN 7150202)

• NanoQuot Operator’s Manual on CD (PN 7151008)

• Spare solenoid valve assembly (PN 76068) that includes valve and tubing to distribution manifold

• Shipping document (PN 94075) that includes a Warranty Statement and Certificate of Compliance and Calibration

• Declaration of Conformity (PN 7151004)

• Valve Maintenance Notice (PN 7151006)

Optional Accessories & Replacement Parts

• External pressure system (compressor):

115 V (PN 7150500)

230 V (PN 7150501)

Note: Alternatively, house air (30 psi inlet) can also be used.

• Reagent bottles, with cap and tubing to the distribution manifold:

250 ml glass supply bottle (PN 48657)

1000 ml glass supply bottle (PN 48658)

• Product Qualification (IQ-OQ-PQ) Package (PN 7150503)

• BioTek NanoQuot Blue Test Dye, 125 ml (PN 7773004)

• Alignment kit for installation of the Bio-Stack™ Microplate Stacker with the NanoQuot (PN 7150001)

• Spare tubing set assembly (PN 71225) that includes 8 tubes (from solenoid valves to the distribution manifold), distribution manifold, and supply tubing



Specifications

Microplates

Microplates: 96-, 384-, and 1536-well plates, standard or half-height depth

Microstrips: 1 x 8

Hardware and Environmental

Dimensions: 12” W x 12.5” D x 8” H (31 cm x 32 cm x 20 cm)

Weight: 15.7 lb. (7.1 kg)

Manifold: 8-tip dispense head; precision solenoid valves

Pressure System: Optional compressor or house air (30 psi inlet)

User Interface: Thumbwheel input, five function keys

Environment: Operational temperature: 10° to 40°C (50° to 104°F)

Relative humidity: 10% - 85% (non-condensing)

Electrical

Power Supply: External 100-240V~ power supply, 50 to 60 Hz

Dispense Accuracy and Precision

The NanoQuot is factory-tested using dispense volumes of 1 µl and 100 nl.

When dispensing water with FD&C #1 blue dye, at dual wavelength, using wavelengths appropriate for the dye:

Dispense Accuracy: ± 7% at volumes ≥ 1 µl ± 12% at 500 nl ± 12% at 100 nl

Dispense Precision: < 7% CV at volumes ≥ 1 µl < 12% CV at 500 nl < 12% CV at 100 nl

Specifications | 9


PC (Optional)

NanoQuot™ PC Control Software: Version 1.00.4 or greater

System Requirements:

• Intel Pentium processor / 266 MHz or equivalent (or the minimum CPU required to run the operating system, whichever is higher)

• Microsoft® Windows 2000 or XP operating systems

• 256 MB of RAM (or the minimum RAM required to run the operating system, whichever is higher)

• Color monitor, 1024 x 768 resolution with 256 colors

• CD ROM drive

• 200 MB of available hard drive space

• Keyboard and mouse

• Serial ports

For operation of the Bio-Stack™ with the NanoQuot™, you will need the following minimum software versions for the Bio-Stack: - Bio-Stack basecode software version 1.25.1 - Bio-Stack™ PC Control Software version 2.00.4

For PC system requirements for the Bio-Stack, refer to “System Requirements” in the Bio-Stack™ PC Control Software Installation Guide.



Product Support & Service

All of BioTek’s products are backed by a superior support staff. If your instrument ever fails to function perfectly, if you have questions about how to use or maintain it, or if you need to send the instrument to BioTek for service or repair, please contact our Technical Assistance Center.

Contacting the Technical Assistance Center (“TAC”)

Our Technical Assistance Center is open from 8:30 AM to 5:30 PM (EST), Monday through Friday, excluding standard U.S. holidays. You can send a fax or an e-mail any time.

Phone: 800-242-4685 (in the U.S.) or 802-655-4740 (outside the U.S.)

Fax: 802-655-3399


Web: www.biotek.com, “Service & Support”

Please be prepared to provide the following information:

• Your name and company information

• A daytime phone or fax number, and/or an e-mail address

• The product name, model, and serial number

• The NanoQuot™ basecode software part number and version number (viewed in the NanoQuot™ PC Control Software’s main screen, Instrument Software field).

• For troubleshooting assistance or instruments needing repair: the specific steps that produce your problem, and any error codes displayed via the PC interface (see also Chapter 6, Troubleshooting, and Appendix A, Error Codes).

Returning Instruments for Service/Repair

If you need to return an instrument for service or repair, please contact the TAC for a Return Materials Authorization (RMA) number and shipping information.

See also Repackaging and Shipping in Chapter 2.

http://www.biotek.com/

Chapter 2

Installation

This chapter includes instructions for unpacking and setting up the NanoQuot™ Microplate Dispenser, installing the dispenser’s components, and repackaging the instrument for shipment.

1: Unpack and Inspect the Instrument........................................12 2: Select an Appropriate Location ..............................................13 3: Remove Shipping Hardware ..................................................13 4: Install the NanoQuot’s Components........................................16 Connect the Tubes and Bottles ..............................................16 Connect the Power Supply ....................................................22 (Optional) Connect the Serial Cable .......................................22 (Optional) Connect the Programmer’s Cable............................23 (Optional) Install Software ...................................................24 5: Turn on the NanoQuot and Verify Performance.........................26 Self-Test ............................................................................26 Fluidics System Test ............................................................26 Installation of the Bio-Stack with the NanoQuot ...........................27 Repackaging and Shipping ........................................................28

12 | Chapter 2: Installation


1: Unpack and Inspect the Instrument

Important! Save all packaging materials.

If you need to ship the NanoQuot™ to BioTek for service or repair, you must use the original packing materials. Using other forms of commercially available packing materials, or failing to follow the repackaging instructions, may void your warranty.

If the original packaging materials have been damaged, contact BioTek for replacements.

See Repackaging and Shipping at the end of this chapter for complete packaging and shipping instructions.

During this process, inspect the packaging, dispenser, and accessories for shipping damage. If the dispenser is damaged, notify the carrier and your manufacturer’s representative. Keep the shipping cartons and the packing materials for the carrier’s inspection. The manufacturer will arrange for repair or replacement of your instrument immediately, before the shipping-related claim is settled.

Perform these steps to unpack and inspect the NanoQuot:

1. Open the outer shipping box.

2. Remove the inner shipping box containing the dispenser and accessories and place it on a level surface.

3. Carefully open the inner shipping box and remove the accessories box.

4. Carefully lift the dispenser with its foam caps out of the box and place it on a level surface.

5. Carefully pull off the foam caps.

6. Remove the dispenser from the plastic bag.

7. Place all packaging materials into the outer shipping box for storage.

Important: The NanoQuot ships with a Final Acceptance data sheet containing calibration data and other settings from the factory. Store this document in a safe place.

2: Select an Appropriate Location | 13


2: Select an Appropriate Location

For optimal operation, install the NanoQuot™ Microplate Dispenser on a level surface in an area where ambient temperatures between 10°C (50°F) and 40°C (104°F) can be maintained. The dispenser is sensitive to extreme environmental conditions. Conditions to avoid are:

• Excessive humidity: Condensation directly on the sensitive electronic circuits can cause the instrument to fail internal self-checks. The NanoQuot requires a relative humidity of 10% - 85% (non-condensing).

• Dust: Efficient microplate transporting may be affected by extraneous particles (such as dust) on the carrier’s linear ways. A clean work area is necessary to ensure smooth plate transporting.

3: Remove Shipping Hardware

Caution! Remove the shipping hardware before turning on the instrument. Replace the hardware before re-shipping the instrument.

The NanoQuot ships with two “motion limiting components” that must be removed before the instrument can be turned on. Figure 4 on page 15 shows the locations of these two items.

1. Remove the fluidics cover:

• Remove the two phillips-head screws attaching the cover to the front of the instrument. The screws are partially hidden under the cover; see Figure 1 on page 14.

• Loosen the thumbscrew attaching the distribution manifold to the inside of the cover (Figure 2).

• Tilt the cover forward and lift it off the two mounting posts (Figure 3 on page 15).

2. The plate carrier is secured to the pressure regulator with a rubber band. Carefully remove the rubber band.

3. One of the dispense head cylinders is wrapped in a red plastic tube. Carefully remove the red plastic tube.

4. Store the rubber band and tube with the rest of the packaging, in case you need to ship the instrument.



Figure 1: Locate and remove two screws holding two corners of the fluidics cover to the front of the instrument

Figure 2: Loosen the thumbscrew attaching the distribution manifold to the fluidics cover

Thumbscrew attaching the distribution manifold to the inside of the fluidics cover

3: Remove Shipping Hardware | 15


Figure 3: Lift the fluidics cover off the two mounting posts

Figure 4: Remove the rubber band (A) and red plastic tubing (B). Note: The supply bottle and fluid path assembly are removed from this image for clarity.

A

B



4: Install the NanoQuot’s Components

Connect the Tubes and Bottles

Important! Review Figure 5 through Figure 9 on the following pages. Identify the different tubes and bottle that came with your dispenser. Note: A 100-ml glass supply bottle is included in the pressure gauge assembly.

Warning! Pressure Hazard! Do not exceed recommended pressures of 30 psi inlet pressure and 15 psi to the supply bottle! Pressures outside the specified limits may cause the bottle to burst and possibly result in injury.

The glass supply bottle that is included with the NanoQuot™ is enclosed in a special net, for your protection, in case the bottle bursts. Do not remove this covering!

The supply bottle tubing has Luer Fittings. Finger-tighten only!

For optimal operation of the NanoQuot™, all tubing and bottles must be properly connected, and the air pressures verified within limits.

4: Install the NanoQuot’s Components | 17


Connect the Waste Tube and Bottle

You will need a waste bottle for the following instructions. (The NanoQuot includes a supply bottle only.)

Rinse the waste tube and bottle with clean, filtered water before connecting them to the NanoQuot.

1. Slide one end of the clear 3/8” waste tube onto the drain fitting on the front of the dispenser (see Figure 5). Ensure that the tubing is not crimped or pinched. If the tubing impedes the free-flow of liquid, the dispenser’s priming trough may flood.

2. Insert the other end of the tube into a waste bottle supplied by you.

3. The NanoQuot’s priming trough empties by gravity. Place the waste bottle a minimum of two feet lower than the drain fitting. Ensure that the tubing does not hang below the waste bottle opening before going into the bottle, or water may pool in the low spot, creating an air lock. If this is the case, use a razor or other cutting tool to shorten the tubing to an appropriate length.

4. Verify that the waste tube is not crimped.

Figure 5: Connect the waste tube to the drain fitting. Note: The drain fitting is black on some instruments.

Waste tube

Drain fitting



Connect the Supply Tube and Bottle

Refer to the figures on the following pages for these instructions.

The pressure gauge assembly containing the supply bottle is shipped already attached to the dispenser. To remove the supply bottle for cleaning, use a flat-blade screwdriver to open the clamp that holds the bottle in place. See Figure 7.

Rinse the supply tube and bottle with clean, filtered water before connecting them.

1. Connect an appropriate fitting to the free end of the supplied 1/8” airline tubing.

• Two fittings are supplied for use with BioTek’s optional compressors (115 V or 230 V). If you are using house air, you may need a different or additional fitting/adapter.

2. Connect the fitting to an acceptable pressure source: BioTek’s optional compressor or house air.

3. Set up the compressor according to the manufacturer’s instructions. If you are using one of BioTek’s compressors:

• Ensure that the drain valve underneath the compressor tank is closed.

• Turn the compressor’s regulator knob fully counter-clockwise (to the left) to close the air flow.

• Turn the on/off switch to the OFF position.

• Plug in the power supply cord.

• Turn the on/off switch to the ON position and let the compressor run until it reaches the automatic shutoff pressure.

4. Attach the supply bottle to the pressure source, by connecting the 1/8” supply line from the compressed air source to the 1/8” line going into the bottle, via their respective Luer fittings. See Figure 6 for an illustration of the tubing connections and Luer fittings.

5. Ensure that the supply bottle is on the same horizontal plane as the dispenser. If it is slightly above the plane:

• Use a flat-blade screwdriver to open the clamp that holds the bottle in place in the pressure gauge assembly (see Figure 7).

• Slide the bottle down until it is on the same plane as the dispenser. (This ensures optimum performance.)

• Close the clamp to secure the bottle in place.

6. Fill the supply bottle with clean, filtered water. Ensure that the bottle’s cap is securely fastened to the bottle.



7. Adjust the air pressure on the compressor or house air to the recommended setting of 30 psi. Do not exceed 30 psi!

• Figure 8 shows one of BioTek’s optional compressors.

8. Turn the NanoQuot’s pressure toggle switch ON. Turn the regulator knob clockwise to increase, or counterclockwise to decrease, the pressure to the supply bottle, as needed (see Figure 9). 12.5 psi is a general recommended setting. Do not exceed 15 psi!

9. Turn the NanoQuot’s pressure toggle switch OFF for now.

10. Verify that the supply tube is not crimped.

Figure 6: Connect the supply tubing

Luer fittings

1/8” air tubing from supply bottle to pressure

gauge assembly

Tube from supply bottle to distribution

manifold

Waste tube from drain fitting to waste

bottle

Luer fittings

Pressure gauge assembly

Air tubing from

pressure gauge

assembly to compressor or house air



Figure 7: Adjust the supply bottle position

Supply tube from bottle to distribution

manifold

Supply bottle holder and clamp

Luer fittings

Luer fittings

Pressure gauge assembly

1/8” air tubing to

supply bottle

1/8” air tubing to house air or

compressor



Figure 8: Adjust the pressure on the compressor. BioTek’s optional 115 V compressor is shown above. Turn the knob clockwise to increase, or counterclockwise

to decrease, the inlet pressure to the NanoQuot™.

Figure 9: Adjust the pressure to the supply bottle

Gauge indicates pressure to the supply bottle

OFF/ON toggle switch, shown in the OFF position

NanoQuot’s regulator knob

Gauge indicates pressure in

compressor tank

Gauge indicates inlet pressure to NanoQuot

Compressor’s regulator

knob



Connect the Power Supply

Warning! Power Rating. The NanoQuot’s power supply must be connected to a power receptacle that provides voltage and current within the specified rating for the system. Use of an incompatible power receptacle may produce electrical shock and fire hazards.

Warning! Electrical Grounding. Never use a plug adapter to connect primary power to the instrument. Use of an adapter disconnects the utility ground, creating a severe shock hazard. Always connect the system power cord directly to an appropriate receptacle with a functional ground.

The NanoQuot™ uses an external 100-240V~, 50-60 Hz power supply. Perform these steps to connect the power supply to the NanoQuot:

1. Connect the power cord to the external power supply.

2. Locate the power inlet on the rear panel of the dispenser (see Figure 10).

3. Plug the rounded end of the power supply’s line cord into the power inlet.

4. Plug the power cord into an appropriate power receptacle.

(Optional) Connect the Serial Cable

The NanoQuot has a 9-pin RS-232 serial port located on the rear panel of the instrument (see Figure 10). The serial port allows the dispenser to be operated using a PC interface (NanoQuot™ PC Control Software for serial commands), to receive data from a computer for purposes of factory or dealer service, or to control the Bio-Stack™ Microplate Stacker.

The package contents for the NanoQuot include one 9-pin to 9-pin female/male serial cable for connecting the dispenser to a host computer. Perform the following steps to connect the serial cable between the two machines:

1. Turn off the computer.

2. Attach the DB9F end of the serial cable to the serial port on the computer.

3. Attach the DB9M end of the serial cable to the serial port on the rear of the NanoQuot (see Figure 10).

4. Tighten the securing screws on both ends of the cable.

For instructions for connecting the Bio-Stack to the NanoQuot, refer to the Bio-Stack Operator’s Manual.



(Optional) Connect the Programmer’s Cable

The NanoQuot™ has an RJ-12 port located on the rear panel of the instrument for connecting a programmer’s cable. This port allows you to download basecode software upgrades to the dispenser.

The package contents for the NanoQuot include a “programmer’s cable” with DB9F to RJ-12 adapter, for connecting the dispenser to a host computer. Perform the following steps to connect the programmer’s cable between the two machines:

1. Turn off the computer.

2. Attach the DB9F end of the programmer’s cable to a serial port on the computer.

3. Remove the access panel from the rear of the NanoQuot, to expose the port for the programmer’s cable.

4. Plug the RJ-12 end of the cable into the port on the rear of the NanoQuot, as shown in Figure 10 below.

Figure 10: Rear Panel of the NanoQuot

DB9F adapter on programmer’s cable

Serial cable Power supply cord Programmer’s cable

Access panel



(Optional) Install Software

You will need to install the NanoQuot™ PC Control Software on a host PC if you wish to display error codes and NanoQuot basecode software information, create and download Liquid Profiles to the dispenser, or use other options that are only available in the PC Software.

Note: The user who is installing the software is required to have Administrator privileges for the Windows operating system. If a user with restricted access attempts to install the software, errors may occur. Contact your organization’s system administrator if you are uncertain about your privileges.

Perform these steps to install the NanoQuot PC Control Software on a host computer:

1. Turn on the computer and start Microsoft® Windows.

• If you are prompted with a Windows password entry dialog, enter a valid password; this helps to ensure a complete installation. If you were not prompted with the Windows password entry, disregard this notice.

2. Place the NanoQuot PC Control Software installation CD in the CD ROM drive.

• If the CD does not launch the InstallShield Wizard automatically, follow steps 3 through 5 below. The InstallShield Wizard instructions begin at step 6.

3. Open the Windows Control Panel and select Add/Remove Programs.

4. When the ‘Add/Remove Programs’ dialog appears, click the Add New Programs button.

5. Click the CD or Floppy button and follow the prompts to “Run Installation Program.”

6. A couple of ‘Preparing to install’ dialogs will briefly appear, followed by the ‘Welcome’ dialog. Click Next. The ‘License Agreement’ dialog will appear.

7. If you accept the terms, click on ‘I accept the terms in the license agreement,’ then click Next to continue. Otherwise, click Cancel to discontinue the software installation.

• If you clicked Next, the ‘Customer Information’ dialog will appear.

8. Enter your User Name and Organization (if they do not appear in the dialog), then click Next. The ‘Destination Folder’ dialog will appear.

9. Specify the folder in which to install the software. If the default location is acceptable, click Next. To choose a different location, click Change, select the desired folder, then click OK > Next. The ‘Setup Type’ dialog will appear.



10. Choose Typical or Custom, (BioTek recommends that you choose ‘Typical’), then click Next.

Note: ‘Liquid Profiles’ and ‘Program Files’ folders will be installed in the default storage location (C:\Program Files\ BioTek\NanoQuot\Liquid Profiles) during a Typical installation.

11. The ‘Ready to Install the Program’ dialog will appear. Click Back if you need to make any changes, otherwise, click Install to begin the installation.

12. When the program confirms that the installation is complete, click Finish to exit the InstallShield Wizard.

Depending upon your Windows version, you may be asked to restart the computer before running the application. If asked, it is imperative that you restart your computer, or the NanoQuot™ PC Control Software and possibly other applications may not run correctly.

Do not open the NanoQuot PC Control Software before you have turned on the NanoQuot™ in step 13 below, or you will receive a communication error.

13. Turn on the NanoQuot™ and wait until the dispenser has completed its Self-Test.

14. Launch the NanoQuot PC Control Software. When the software’s main screen appears, verify that the dispenser’s Serial No. and Instrument Software information (basecode part number and version) are displayed and that the correct COM Port is selected.

15. Click Test Communications.

• If the software does not display the serial number and instrument software version, verify that the instrument is turned on and the serial cable is connected properly. Click ‘Test Communications’ again.

• If you receive the ‘Communication test was successful’ dialog, click OK.

• If the test is not successful, try a different COM port or check the serial cable connections. Refer also to the Troubleshooting section of the PC software’s Help system for assistance.



5: Turn on the NanoQuot and Verify Performance

Self-Test

Turn on the NanoQuot™ by pressing the On/Off switch on its left side. The keypad and thumbwheels will remain inactive while the dispenser performs a Self-Test. The Self-Test includes a homing sequence (x- and y-axis home positions), to ensure that the microplate carrier track is clear of obstructions (e.g., Bio-Stack claw/gripper).

• If the Self-Test passes, the green Power LED will illuminate, and the dispenser is ready for use.

• If the Self-Test fails, the red Alert LED will flash and the dispenser will retain an error code. To retrieve an error code, you will need a host computer connected and the NanoQuot™ PC Control Software installed on the computer, as instructed in the previous sections. At the software’s main screen, click Get System Status to retrieve the error code, and then look up the code in Appendix A, Error Codes.

Note: When the dispenser is on, you can run the Self-Test manually by pressing and releasing the Stop key.

Fluidics System Test

Note: These instructions apply to the NanoQuot’s front panel. The NanoQuot PC Control software can also be used to perform this test.

Perform these steps to test the fluidics system:

1. If you have not already done so:

• Fill the supply bottle with clean, filtered water.

• Securely fasten the bottle cap.

• Turn on the compressor or house air. If using the compressor, allow it to run until it reaches its automatic shutoff pressure. Set the compressor or house air to 30 psi. Do not exceed 30 psi!

• Turn on the NanoQuot. Turn the NanoQuot’s pressure toggle switch On. Set the pressure to approximately 12.5 psi.

2. Set the Plate Type switch to 96/LP1.

3. Set the Mode switch to Manual.

4. Set the Volume to 40 µl (40.00).

5. Set the Cols to 12.

Installation of the Bio-Stack with the NanoQuot | 27


6. Press the Prime button. The fluid lines should begin to fill with water. Repeat the Prime until water consistently dispenses out of all 8 valves into the priming trough.

• Verify that there are no leaks in the tubing. If there are any leaks, tighten all fittings.

• If the lines are not filling with water and/or if there are large air bubbles in the tubing, verify that the supply tubing is correctly installed and then check the pressure from the compressor or house air and the NanoQuot’s pressure gauge.

• If/when the supply bottle volume drops about halfway:

Turn the NanoQuot’s pressure toggle switch Off to depressurize the bottle.

Unscrew the cap on the supply bottle, taking care not to pull the supply tube out of the remaining fluid.

Unscrew the cap on the supply bottle, taking care not to pull the supply tube out of the remaining fluid. Refill the supply bottle or change to a full spare bottle, and securely fasten the cap.

Turn the NanoQuot’s pressure toggle switch On and ensure the pressure is set to 12.5 psi.

7. When finished, you can either leave the fluid in the lines, or Purge the lines. See “Manual Control of the NanoQuot “ in Chapter 3 for instructions on Purging.

For information regarding dispenser performance, see Chapter 4, Instrument Qualification.

Important! Before operating the NanoQuot, review the General User Guidelines in Chapter 3, Operation. These guidelines include necessary steps to perform before running any program, information on performing periodic maintenance, and points to consider when creating or editing dispenser programs.

Installation of the Bio-Stack with the NanoQuot

Special alignment hardware and serial cable connections must be installed before the NanoQuot can control the Bio-Stack. Refer to the Bio-Stack Operator’s Manual for installation instructions.



Repackaging and Shipping

If you need to return the NanoQuot™ for service or repair, be sure to use the original packing. Other forms of commercially available packing are not recommended and can void the warranty. If the original packing materials have been damaged or lost, contact BioTek for replacement materials (see Product Support & Service in Chapter 1 for contact information).

Warning! If the NanoQuot has been exposed to potentially hazardous material, decontaminate it to minimize the risk to all who come in contact with the instrument during shipping, handling, and servicing. Decontamination prior to shipping is required by the U.S. Department of Transportation regulations.

Important! Perform the following steps in the order presented before repackaging the instrument for shipment.

1. Decontaminate the dispenser and its accessories as necessary. Refer to the Decontamination section in Chapter 5, Preventive Maintenance, for complete instructions.

2. Turn off the NanoQuot and disconnect the power supply cord.

3. Important! Replace the shipping hardware:

• Remove the fluidics cover. Wrap the red plastic tubing around one of the dispense head cylinders (see Figure 4 on page 15).

• Slide the plate carrier all the way to the left. Secure the plate carrier to the pressure gauge using the rubber band as shown in Figure 11.

Repackaging and Shipping | 29


Figure 11: Securing the plate carrier for shipment (fluid path assembly removed for clarity)

4. Repackage the instrument and accessories as described below. Failure to comply with these packaging instructions may void the instrument’s warranty.

• Place the dispenser into a plastic bag.

• Slide the foam caps onto the dispenser.

• Carefully set the dispenser with its foam caps into the inner shipping box.

• Put the accessories in the accessory box, and place the accessory box inside the inner shipping box.

• Carefully lower the inner shipping box into the outer shipping box.

• Securely tape the inner and outer boxes closed.

• Record the RMA on the side of the outer shipping box.

5. Contact BioTek for a Return Materials Authorization number (RMA) and shipping information. Chapter 1 contains contact information.

When obtaining the RMA, explain whether the NanoQuot requires calibration, cleaning, periodic maintenance, warranty work, and/or repair. Make a note of any error messages that were displayed via the PC interface, and their frequency.

6. Write the RMA number on the outside of the shipping box.

7. Provide BioTek with the name and e-mail address, fax or telephone number of a person who may be contacted if questions arise.

8. Insure the instrument for full value.

Chapter 3

Operation

This chapter provides instructions for operation of the NanoQuot™ Dispenser, using either the keypad or the NanoQuot™ PC Control Software. A description of the Dispenser’s front panel and general usage guidelines are also provided.

Overview................................................................................32 Manual Control vs. PC Control ...............................................32 NanoQuot User Interface ..........................................................33 Rocker Switches..................................................................33 Thumbwheels .....................................................................33 Keypad Functions and LEDs ..................................................34 General Usage Guidelines .........................................................34 Before Running Any Program ................................................34 Maintenance.......................................................................36 Programming Considerations ................................................36 Adjust the Dispense Head.....................................................36 Define A1 Locations .................................................................37 Manual Control of the NanoQuot™..............................................38 Purge ................................................................................38 Prime ................................................................................39 Dispense............................................................................40 Step ..................................................................................41 PC Control of the NanoQuot™....................................................42 Purge ................................................................................43 Prime ................................................................................43 Clean ................................................................................44 Dispense Programs..............................................................45 Liquid Profiles .....................................................................47

32 | Chapter 3: Operation


Overview

Manual Control vs. PC Control

Manual control of the NanoQuot™ (via the keypad, thumbwheels, and rocker switches) allows you to run Prime, Purge, Dispense, and Step programs, and control a Bio-Stack™ instrument.

The NanoQuot PC Control Software allows you to run Prime, Purge, Clean, and Dispense programs, as well as create Liquid Profiles (LPs), download LPs to the dispenser, define the A1 well location, and perform many other tasks.

This chapter provides instructions for both manual and PC control of the NanoQuot. Regardless of which mode you use to control the dispenser, you should review the information in NanoQuot User Interface and General Usage Guidelines on the following pages before operating the Dispenser.

If you have not already done so, we suggest you turn to page 4 now and read the introductory material under Liquid Profiles. See also the information on liquid profiles in this chapter, starting on page 47.

If you purchased the Bio-Stack to operate with the NanoQuot™, refer to the instructions in your Bio-Stack Operator’s Manual for operation of the Bio-Stack with the Dispenser.

Note: To control the Bio-Stack with the NanoQuot, the Mode rocker switch on the Dispenser must be set to Bio-Stack.

NanoQuot User Interface | 33


NanoQuot User Interface

The user interface on the front panel of the NanoQuot™ includes thumbwheels, rocker switches, and a 5-key keypad, as shown below.

Figure 12: NanoQuot Front Panel

Rocker Switches

Use the rocker switches to select the plate type (96, 384, or 1536) or Liquid Profile (LP1, LP2, LP3), and to select Manual or Bio-Stack mode.

Thumbwheels

Use the thumbwheels to select the Volume and ending column (Cols).

For a Prime program, the Cols thumbwheel is used to select repetitions (number of replicate dispenses).

Manual/Bio-Stack Mode rocker switch

Volume/Columns thumbwheels

Plate type/Liquid Profile rocker switch

Alert and Power LEDs

Keypad with functions listed above/below



Keypad Functions and LEDs

Most of the keypad functions are listed above the NanoQuot’s keypad. To make a selection, press the key below the menu function.

Certain functions, such as purging or selecting an on-board liquid profile, are listed below the keys. To activate these functions, hold the key down for approximately 5 seconds. When the red Alert LED flashes to indicate the function has been selected, release the key.

Press the Start key to begin running a dispenser program. Press the Stop key to stop running (abort) a dispenser program.

General Usage Guidelines

Important! Please read the following Guidelines carefully before operating the NanoQuot.

Before Running Any Program

• Fill the supply bottle with a sufficient volume of clean, filtered liquid. Ensure that the supply tube is in the liquid and the tube end does not contain an air bubble. Do not let the supply bottle run empty during an operation.

• The supply tube should extend to the bottom of the bottle and be cut at the end (we recommend at an angle) to ensure free flow of liquid at the bottom of the supply bottle.

• Check the external tubing connections for kinks and clogs.

• Make sure the bottles are clean and do not contain any particles or mold.

• If there are any air bubbles in the tubing, run a Purge program using clean, filtered water before running another program. Multiple Purges may be required for some solutions. Use a pressure setting of 12.5 psi. Note: Dispense accuracy and precision can be affected if there are air bubbles in the tubing, and the Purge program is an effective way to eliminate them. The Purge takes approximately 18 minutes to complete.

• To avoid creating air bubbles every time the supply bottle is filled, make a mark halfway down the bottle and refill when the fluid level has dropped to that point. Unscrew the cap and let it hang over the side just enough to avoid emptying the inside tube and enough to refill the bottle. Leave a droplet of fluid at the end of the tube; do not shake it off.

General Usage Guidelines | 35


• If using the NanoQuot daily, keep the fluid lines wet with filtered, deionized water and pressurized overnight.

• When placing a microplate into the carrier, make sure that well A1 is in the left rear corner as you face the front of the dispenser. Make sure that the plate is firmly seated in the carrier. If necessary, use the two carrier adjustment brackets to best position the plate:

Using your fingers, loosen (but do not remove) the screws that attach the two adjustable brackets to the plate carrier.

Adjust the brackets to accommodate the plate width and length, so the plate fits snugly in all four corners.

Finger-tighten the screws in the brackets.

• After changing to a different plate type, you should perform a test dispense to see if the NanoQuot is correctly dispensing to well A1. If you find that you need to adjust the A1 location, open the NanoQuot™ PC Control Software and click Define A1 Locations. Refer to the instructions in Define A1 Location on page 37.

• If fluid collects on the tips between runs or as the result of a run, use the Tip Prime Repetitions feature in the NanoQuot™ PC Control Software to remove it. You may need to wipe the tips with a lint-free cloth; see Clean the Dispense Head in Chapter 5. Tip priming will also resupply fluid to the tips, if the instrument has been sitting idle for a while.

If you are using expensive reagent, you may decide that the reagent costs more than it is worth to prime the tips.

• Use clean, filtered reagent for daily maintenance routines.

• Ensure that the fluid being used in the reagent is standardized, e.g., not from a faucet one day (very aerated), and a stock bottle the next (very low air).

• Watch for reagents that show outgassing overnight (indicated by bubbles in the inlet line in the morning). Run a Purge if you detect any air bubbles.

• Watch for pressure going down over time (possibly because the compressed air is not turned on). Ensure that the pressure regulator is regulating properly (it should regulate down to 5 psi).

• Before running a plate, ensure that the dispense head is positioned close enough to the plate. Refer to the instructions in Adjust the Dispense Head, on the following page.

• Ensure that you standardize the read method. For instance, if you are checking dispense accuracy, the evaporation rate of alcohol may affect results. Volatile compounds (e.g., alcohol, acetonitrile) will evaporate quickly. Minimize the time between the dispense routine and weighing during calibration.



Maintenance

Perform Daily Maintenance and Periodic Maintenance regularly. See Chapter 5, Preventive Maintenance, for more information. Periodic Maintenance includes:

• Purging and Priming at the start of the day, and Cleaning at the end of the day, using clean, filtered water

• Cleaning the bottles and tubes

• Cleaning all external surfaces including the fluidics cover and plate carrier system

• Cleaning the dispense head

• Decontamination of external surfaces and the fluid path

Programming Considerations

• Ensure that the correct Liquid Profile has been created and is selected.

• Ensure that the correct Plate Type and Volume are selected.

• Ensure that the correct starting and ending Columns are specified.

Adjust the Dispense Head

Before dispensing to a microplate (especially low volume plates), you may need to adjust the dispense head to vertically position it closer to the plate:

1. Place the microplate on the carrier.

2. If the fluidics cover is attached to the instrument, remove the cover (see the photos under “Remove Shipping Hardware” in Chapter 2):

Remove the two screws attaching the cover to the front of the instrument. The screws are partially hidden under the cover.

Loosen the thumbscrew attaching the distribution manifold to the inside of the cover.

Tilt the cover forward and lift it off the two mounting posts and set aside.

3. Loosen the thumbscrew that raises and lowers the dispense head (Z-axis).

4. Move the dispense head up or down, as necessary until it is at the desired height for the plate.

5. Tighten the thumbscrew on the dispense head.

6. Replace the fluidics cover, if desired, or leave it off for operation of the dispenser.

Define A1 Locations | 37


Define A1 Locations

The NanoQuot™ is factory-calibrated for standard 96-, 384-, and 1536-well microplates. If you are using a non-standard plate, and/or you observe that the NanoQuot is not dispensing in the centers of the microwells, you may need to adjust the “A1” well location. Use the Define A1 Locations feature in the NanoQuot™ PC Control Software to “tell” the NanoQuot the exact position of well A1. The goal is to align the rear-most dispense tube over the exact center of well A1. You will adjust the dispense head in the x- and y-axes, and then run a test dispense, observing the location of the dispensed fluid.

1. Fill the reagent bottle with clean, filtered water or test fluid.

2. Place the plate on the carrier. If necessary, use the two adjustment brackets to best position the plate:

Using your fingers, loosen (but do not remove) the screws that attach the two brackets to the plate carrier.

Adjust the brackets to accommodate the plate width and length, so the plate fits snugly in all four corners.

Finger-tighten the screws in the brackets.

3. Remove the fluidics cover to access the thumbscrew and lower the dispense head to approximately 1/8” above the plate.

4. Click Define A1 Locations at the main screen.

5. In the Position Verification section, use the drop-down list to select the Plate Type: 96-, 384-, or 1536-well.

6. Increase the number of Repetitions to a sufficient number so you can easily observe the action (10-15 is recommended).

7. Enter the Volume of fluid to be dispensed. Reduce the volume when you have low confidence of the dispense head’s position above the plate. Later, when you are more certain about the alignment, you can increase the volume dispensed to make it easier to visually assess the performance.

8. Click the Check Position button. Visually verify that the A tip is centered over well A1, as the NanoQuot dispenses the defined volume to the well for the defined number of repetitions.

9. If not centered, go to the Coordinate Settings section and adjust the X- or Y- Coordinates to best position the plate under the valves.

X-axis: increase the value to move left, decrease to move right. Y-axis: increase the value to move forward, decrease to move back.

• The X-Coordinate is right to left looking at the instrument from the front.

• The Y-Coordinate is back to front looking at the instrument from the front.

• Values increase as components move away from the home position.



• X home is the plate platform all the way to the right.

• Y home is the head closest to the instrument.

10. Click Download Settings and re-check the positions.

11. Repeat steps 6-10 until you are satisfied with its precision.

12. Finally, run a test program to validate your settings.

Manual Control of the NanoQuot™

Warning: Do not let air follow fluid into the supply tubing during an operation. This will cause the fluid to be emitted as an aerosol. Depending on the fluid, this could be hazardous to your health.

Important! When using deionized water as a reagent, fluid may collect on the tips. If this is a problem, distilled water could be used instead.

This section provides instructions for operation of the dispenser using just the keypad, thumbwheels, and rocker switches.

Purge

The Purge routine is designed to remove all air from the tubing. It pushes fluid through the tubing at several different frequencies to ensure air bubbles of various sizes are eliminated. The fluid volume, number of repetitions and other parameters are pre-defined and cannot be reset with the keypad controls or PC software.

To run a Purge:

1. Fill the supply bottle with the desired fluid (e.g., clean, filtered water).

2. Turn the pressure on the compressor or house air to 30 psi.

3. Turn the NanoQuot’s pressure gauge to 12.5 psi.

4. Select Manual mode.

5. Set the Cols value to 9 to purge all valves, or from 1-8 to purge valves A-H individually. Note: The Volume is pre-defined.

6. Press the Purge key. The program will run for approximately 18 minutes. If you wish to abort the program, press the Stop key.

Manual Control of the NanoQuot™ | 39


Prime

Run a Prime program to prime the system with fluid before running a dispense program. Priming is required before the first dispense of the day, and recommended after a long delay between dispense runs. It is especially important to run the Prime after changing the type of liquid or refilling the supply bottle.

To run a Prime:

1. Turn off the pressure to the supply bottle using the toggle switch. Fill the bottle with the desired reagent. Try not to let air into the pickup at the bottom of the tubing.


3. Select a desired Liquid Profile (this is only necessary when changing the Liquid Profile selection):

Use the Plate/Liquid Profile rocker switch to select the desired Liquid Profile (LP1, LP2, or LP3). Note: If you do not know which liquid profiles have been downloaded to the dispenser, we recommend you re-download the desired profiles using the NanoQuot PC Control Software.

Press and hold the Cal key until the Alert light repeatedly flashes. The Alert light will then verify the Liquid Profile selected by blinking once for LP1, twice for LP2, and three times for LP3. The carrier will then home. If necessary, reset the rocker to the correct plate type.

4. Set a desired volume with the Volume thumbwheels.

Note: If you select a volume that is higher than defined in the current Liquid Profile, the Dispenser will automatically run a Clean program (approximately 2.5 ml when dispensing water from all 8 tips at 12.5 psi).

5. Set the number of replicate dispenses (repetitions) with the Cols thumbwheels.

Repetitions = the number of times that each tip dispenses fluid.

6. Press the Prime key. The actual volume dispense is equal to the Volume x Repetitions x 8 (the number of tips).

To completely replace fluid in the tubing, set the Volume and Repetitions combination to be greater than or equal to 2 ml. The NanoQuot’s dead volume is approximately 1.5 ml.



Dispense

Important! Prior to running a Dispense program, it is important that the dispenser has been adequately purged and primed. Failure to do so may leave residual air in the fluid lines and valves that will affect the dispense volumes.

First, run the Purge program with clean, filtered water. This takes about 18 minutes and will eliminate any air from the system. Now, replace the water with the desired reagent, being careful not to introduce air into the pickup at the bottom of the tubing. Next, perform a Prime sufficient to clear the lines of the water. The Dispenser is now ready to run a Dispense program.

To run a Dispense:



Use the Plate/Liquid Profile rocker switch to select the desired Liquid Profile (LP1, LP2, or LP3). Note: If you do not know which liquid profiles have been downloaded to the dispenser, we recommend you re-download the desired profiles using the NanoQuot PC Control Software.

Press and hold the Cal key until the Alert light repeatedly flashes. The Alert light will then verify the Liquid Profile selected by blinking once for LP1, twice for LP2, and three times for LP3. The carrier will then home.

3. Set a desired volume with the Volume thumbwheels. Some examples:

XX.10 = 0.1 µl (100 nl) X1.00 = 1 µl 10.00 = 10 µl

4. Set the ending column number with the Cols thumbwheels.

The starting column is pre-defined as column 1. The starting column can only be adjusted when running a program with the NanoQuot™ PC Control Software.

5. Select the plate type with the plate type (96, 384, 1536) rocker switch.

6. Press the Start key. If you wish to abort the program, press the Stop key.

Manual Control of the NanoQuot™ | 41


Step

Step programs are defined in the same way as Prime and Dispense programs. Step programs are unique, however, because they run one column at a time and wait for your to continue.

Note: This is a manual operation only. Step programs cannot be run via the NanoQuot™ PC Control Software.

To run a Step:



Use the Plate/Liquid Profile rocker switch to select the desired Liquid Profile (LP1, LP2, or LP3).

Press and hold the Cal key until the Alert light repeatedly flashes. The Alert light will then verify the Liquid Profile selected by blinking once for LP1, twice for LP2, and three times for LP3. The carrier will then home.

3. Set a desired volume with the Volume thumbwheels.

4. Set the ending column number with the Cols thumbwheels.

5. Select the plate type with the plate type (96, 384, 1536) rocker switch.

6. Press the Step key. The unit will advance to the first column and dispense per the defined program. The dispenser will then wait for Step to be pressed again, and will deliver a liquid volume as defined (or redefined) to the next column. This can be repeated until it reaches the Cols selected, when it will then home.

The Stop button may be pressed to end the sequence early.



PC Control of the NanoQuot™

Warning: Do not let air follow fluid into the supply tubing during an operation. This will cause the fluid to be emitted as an aerosol. Depending on the fluid, this could be hazardous to your health.

Important! When using deionized water as a reagent, fluid may collect on the tips. If this is a problem, distilled water could be used instead.

This section provides the following instructions for operation of the dispenser using the NanoQuot™ PC Control Software:

• Prime, page 43

• Purge, page 43

• Clean, page 43

• Create a Dispense Program, page 45

• Run a Dispense Program, page 46

• Learn about Liquid Profiles and see a list of the Liquid Profiles that come with the NanoQuot, page 47

• Create a Liquid Profile, page 48

• Modify a Liquid Profile, page 51

• Download a Liquid Profile to the Dispenser, page 51

• Verify a Liquid Profile, page 52

In addition, refer to the Help system in the NanoQuot PC Control Software for additional information on all of the topics listed above.

PC Control of the NanoQuot™ | 43


Purge

The Purge routine is designed to remove all air from the tubing. It pushes fluid through the tubing at several different frequencies to ensure air bubbles of various sizes are eliminated. The fluid volume, number of repetitions and other parameters are pre-defined and cannot be reset with the keypad controls or PC software.

To run a Purge:

1. Fill the supply bottle with the desired fluid (e.g, clean, filtered water).

2. Turn the pressure on the compressor or house air to 30 psi.

3. Turn the NanoQuot’s pressure gauge to 12.5 psi.

4. Select the tips (Tip Selections) for the valves you wish to purge. Select 1-8 for tips A-H, or 9 for all tips.

5. Click Purge. The program will run for approximately 18 minutes. If you wish to abort the program, press the Stop key on the dispenser.

Tip Selections is the only control available for the Purge routine (Volume and Repetitions are pre-defined). This feature is also helpful for troubleshooting a valve.

Prime

Run a Prime program to prime the system with fluid before running a dispense program. Priming is required before the first dispense of the day, and recommended after a long delay between dispense runs. It is especially important to run the Prime after changing the type of liquid or refilling the supply bottle.

To run a Prime:

1. Turn off the pressure to the supply bottle and fill it with the desired reagent. Try not to let air into the pickup at the bottom of the tubing.

2. Turn on the pressure to the supply bottle to the desired psi.

3. Download the Liquid Profile for the reagent, if necessary. (See Download a Liquid Profile to the Dispenser on page 51.)

4. At the PC Software’s main screen, in the Fluidic Line Operation area, select the A1 Position Plate Type.

5. Enter the desired Volume (the volume cannot exceed the maximum volume of the Liquid Profile).

6. Set Repetitions to adequately fill tubing with reagent.

7. Click Prime.

To completely replace fluid in the tubing, set the Volume and Repetitions combination to be greater than or equal to 2 ml. The NanoQuot’s dead volume is approximately 1.5 ml.



Clean

The Clean routine is designed to flush the tubing with the desired cleaning fluid. A Clean should be run at the end of each day for daily maintenance, and whenever the dispenser needs to be decontaminated.

To run a Clean:

1. Turn off the pressure to the supply bottle and fill it with clean, filtered water or another recommended cleaning fluid.

2. Set the pressure gauge to 12.5 psi.

3. At the PC Software’s main screen, in the Fluidic Line Operations area, select the Plate Type.

4. Set Repetitions to adequately flush the tubing (10 is recommended). Approximately 2.5 ml per repetition is dispensed when dispensing water from all 8 tips at 12.5 psi.

5. Click Clean. If you wish to abort the program, press the Stop key on the dispenser.

6. If you did not use clean, filtered water during the clean routine, run another Clean with clean, filtered water.



Dispense Programs

Create a Dispense Program

Important! The NanoQuot™ cannot determine if the requested dispense Volume is appropriate for the selected Plate Type (maximum well volume varies greatly from one plate type to another). You must keep track of how much fluid will be dispensed to the wells and ensure the requested dispense volume will not overflow the wells.

Six dispense programs are included with the NanoQuot: 96-well Full/Half Plate, 384-well Full/Half Plate, and 1536-well Full/Half Plate. The programs are installed on the host computer in C:\Program Files\BioTek\NanoQuot\ Program Files when you perform a “Typical” installation of the NanoQuot™ PC Control Software. You can modify these files as needed, and then save them under another file name. (The installed files are read-only and should not be overwritten.)

To create a Dispense program:

1. At the main screen, click Program File Management.

2. Select File > New.

3. Enter a Program Name.

4. Select a Plate Type.

5. Enter the Volume to dispense to each well. (The dispense volume cannot exceed the highest defined value in the Volume allowed for current profile field.)

6. If desired, change the setting for Start Column. (The default setting starts dispensing to the first column of the plate.)

7. Define the # Columns to dispense into. The NanoQuot will start at the column defined in the previous field and continue dispensing to the number of columns defined here.

8. If desired, change the setting for Tip Prime Repetitions. (The default setting is 2.) Tip priming is performed immediately before the dispense and dispenses the selected volume over the drain trough.

9. If desired, change the Tip Selections. (Unchecked tips will not dispense.)



10. Optionally, add a description or special instructions for running this program in the Notes box.

11. Select File > Save and give this program a unique filename to use it again in the future. The file will be saved with a .pgm extension.

Run a Dispense Program

Important! Prior to running a Dispense program, it is important that the dispenser has been adequately purged and primed. Failure to do so may leave residual air in the fluid lines and valves that will affect the dispense volumes.

Run the Purge program with clean, filtered water. This will eliminate any air from the system. Replace the water with the desired reagent, being careful not to introduce air into the pickup at the bottom of the tubing. Perform a Prime sufficient to clear the lines of the water. The Dispenser is now ready to run a Dispense program.

To run a Dispense program:

1. Review and change, if necessary, the currently loaded Liquid Profile.

2. Prime the dispenser.

3. Place the defined plate type in the carrier.

4. At the main screen, click Program File Management.

5. Select File > Open.

6. Select a dispense program (.pgm file).

7. Check the settings and change them if necessary.

The Volume cannot exceed the parameters defined for the currently loaded Liquid Profile.

8. Click Run Program when you are ready.

Note: If you wish to abort the program, press the Stop key on the dispenser’s keypad.



Liquid Profiles

BioTek has developed a set of Liquid Profiles that use 13 data points to precisely describe the dispensing variables of specific liquids when using the NanoQuot™. The NanoQuot uses the profiles to ensure dispensing precision and accuracy for a liquid at a defined concentration.

A Liquid Profiles folder is included on the NanoQuot™ PC Control Software installation CD, and is automatically installed on the host computer when you perform a “Typical” installation of the PC Software. The table below is a list of the Liquid Profiles included in the folder.

Fluid Pressure

Deionized Water @ 12.5 psi

BSA 1% @ 4 psi @ 12.5 psi

DMSO 100% @ 4 psi @ 12.5 psi

Ethanol 100% @ 12.5 psi

Glycerol 10% @ 12.5 psi

Glycerol 40% @ 12.5 psi

PBS @ 4 psi @ 12.5 psi

PEG 400 24% @ 12.5 psi

PEG 400 50% @ 12.5 psi

TE @ 4 psi @ 12.5 psi

BSA = Bovine Serum Albumin

DMSO = Dimethyl Sulfoxide

PBS = Phosphate Buffered Saline

PEG = Polyethylene Glycol 400

TE = 10 mM Tris, 1 mM EDTA

The 4 psi Liquid Profiles are specifically designed for low-volume 384-well V-bottom plates, to avoid splashing.

Up to five Liquid Profiles can be stored on the NanoQuot. Two of these profiles are pre-defined: a Unit Calibration Profile (for use when creating Liquid Profiles), and a Default Liquid Profile (a read-only file that shows relative values for an aqueous liquid). You can program and select the other three profiles via the PC Software and download them to the dispenser.



The NanoQuot™ is shipped with the following Liquid Profiles on-board:

• Unit Calibration Profile

• Default Liquid Profile

• DI water, 12.5 psi (Profile 1)

• DMSO 100%, 12.5 psi (Profile 2)

• Glycerol 40%, 12.5 psi (Profile 3)

Due to differences in % humidity, temperature, and other factors (such as slight variations from one instrument to another), BioTek recommends that you use the Liquid Profiles as a starting point. You will need to create your own Liquid Profiles for fluids that are not included in the Liquid Profiles developed by BioTek, or if the liquid profiles provided by BioTek are not performing adequately for your environment.

Create a Liquid Profile

Prerequisites:

Perform steps 1-7 if the system is dry and/or bubbles are visible in the tubing.

1. Have ready for use a precision balance with readability of 0.001 grams, one 96-well format 1x8 microstrip for each data point (13), and a 96-well format microstrip holder.

2. Make sure the supply bottle has sufficient liquid.

3. Load the Unit Calibration Profile:

At the NanoQuot PC Control Software’s main screen, click Liquid Profile Management.

Using the drop-down button in the Current Profile Selection box, select Unit Calibration Profile.

Click Use As Current. The Current column should now display Unit Cal.

4. In the PC Software’s main screen, set the Fluidic Line Operations/Settings: A1 Position Plate Type to 384-Well, Volume to 1.00 μl, Repetitions to 1. Select all Tip Selections check boxes.

5. Purge the dispenser.

Set the input pressure to the NanoQuot to 30 psi.

Fill the supply bottle with 100 ml of clean, filtered water.

Turn the pressure switch to On.

Set the output pressure to the supply bottle to 12.5 psi.



Click the Purge button in the PC Software’s main screen.

Verify that water flows freely from all 8 valves.

Wait for the Purge operation to complete (approximately 18 minutes).

6. Verify the Purge:

Place a plate cover on the plate platform.

Lower the dispense head as close to the plate cover as possible, without actually touching the cover.

Click the Program File Management button.

Set the Program File Management settings: Plate Type to 384-Well, Volume to 100 nl, Start Column to 1, # Columns to 24, Tip Prime Repetitions to 2. Select all Tip Selections check boxes.

Click the Run Program button.

Visually verify that dispensed droplets appear uniform in size on the plate cover. If they do not appear uniform in size (position might vary), then repeat the purging operation and purge verification.

Exit the ‘Program File Management’ dialog to return to the PC Software’s main screen.

7. Prime the dispenser before the first run, setting the Volume and Repetitions combination to be greater than or equal to 2 ml, remembering that the Volume setting cannot exceed the maximum volume of the Unit Calibration Liquid Profile (60 µl).

To create a new Liquid Profile specifically for your NanoQuot and a certain fluid:

1. If you have not already done so, Prime the dispenser with the solution of interest.

2. At the main screen, click Liquid Profile Management.

3. Select File > New (or click the New Liquid Profile icon).

4. Assign this file a unique Liquid Profile Name.

5. Click Define Profile to open the ‘Liquid Profile Preparation’ dialog.

6. Check the Pressure Used setting and revise as needed. Set the NanoQuot’s pressure gauge to match your setting.

7. Enter the Specific Gravity of the liquid.

8. Weigh 13 microstrips and record weights in the Initial Weight (g) column.

You can either weigh all 13 strips before conducting the remaining steps, or weigh them one at a time: initial weigh, dispense, and final weigh.



9. Review and revise, if needed, the Requested Volume Setting.

10. Volumes must be entered in ascending order, from lowest to highest volume. Take note of the Time (msecs) column of the table. It is set by the Requested Volume: 1000 msecs for every 1 µl of fluid. This is the time the valve will stay open.

11. Review and revise, if needed, the Repetitions.

Increase the Repetitions for low volumes to increase your confidence of the weight results. Lower the Repetitions for higher volumes to prevent overfilling the plate.

12. Double-click the row heading number to duplicate the settings in the Calibration Control area at the bottom of the screen. Make sure the correct A1 Position Plate Type is defined and the Current Profile says Unit Calibration Profile.

13. Place the plate with the 1st strip wells in column 1 onto the plate carrier.

14. Remove the fluidics cover to access the thumbscrew and raise the dispense head to the correct height.

15. Adjust the bottle pressure to the value selected in Pressure Used. Do not exceed 15 psi to the bottle.

16. Click the Dispense button.

17. Observe the tips during the dispense to determine if the fluid is flowing without forming a ball at the end of the tips.

If fluid is balling up on the tips, carefully wipe them clean with a lint-free swab, and change the current pressure setting. Raise the pressure (psi) on the Dispenser for more viscous fluids and try again. Be careful when using low-volume plates, e.g., 384-well V-bottom; too high a pressure setting causes the fluid to splash out of the wells. Be sure to update the Pressure Used field of the Liquid Profile record. Note: All 13 strips must be measured using the same pressure.

If a single tip valve is dispensing too high or too low, you may need to change the valve bias setting (at the Liquid Profile Management screen, select Utilities > Valve Bias Settings and click the Help button for guidance).

18. After the dispense, carefully remove the strip, weigh it, and record the weight in the Final Weight column.

19. Repeat the process, from step 8, for each of the 13 strip wells.

20. Click OK to save and close the ‘Liquid Profile Preparation’ dialog.

21. Click File > Save (or click the Save icon) at the ‘Liquid Profile Management’ dialog. The profile will be saved with a .pfl extension.



Modify a Liquid Profile

To modify a Liquid Profile:



3. To make a copy of the open file, select File > Save As and assign it a unique file name; otherwise, skip this step.

The installed files are Read-Only.

4. If you performed Step 2, re-enter the file name as the Liquid Profile Name.

5. Click Define Profile.

6. Make changes as needed. Follow the instructions to Create a Liquid Profile on page 48.

Download a Liquid Profile to the Dispenser

To download a Liquid Profile to the Dispenser:



3. Locate and select the desired Liquid Profile. The Liquid Profile Name and details will be displayed in the Opened Liquid Profile area.

You can modify the profile, if necessary.

4. At the bottom of the Opened Liquid Profile area, in the Data Transfer Control box, use the buttons to select a profile position: Profile 1, 2, or 3.

5. Click Download Profile.

6. At the bottom of the On-Board Liquid Profiles area, in the Current Profile Selection box, use the drop-down button to select the profile defined above: Profile 1, 2, or 3.

7. Click Use As Current.



Verify a Liquid Profile

To verify a Liquid Profile:

1. In the Data Transfer Control section of the Liquid Profile Data File management window, select one of the Liquid Profile radio buttons (Profile 1 is the default).

2. In the Current Profile Selection sections, select the Profile you just transferred from the pull-down menu.

3. Click the Download Profile button to overwrite the selected profile on the NanoQuot™ with the calibration profile you just created.

4. Click the Use As Current button to select the recently downloaded profile as the NanoQuot’s active profile. The Current column in the On-Board Liquid Profiles section should now display the selected profile in the header, and contain the recently created profile data.

5. Click the Define Profile button to open the ‘Liquid Profile Preparation’ dialog.

6. Confirm that in the lower right corner of the screen, the Current Profile is the Profile you selected.

7. Add to the Liquid Profile Data Filename “Verification.”

8. Enter in the Requested Volume Setting column the volume settings you would like to verify.

9. Repeat the steps from the Calibration Procedure for the rows you are verifying.

10. Run once at default pressure in Pressure Used.

11. If Dispensed Volume is higher than Requested Volume Setting (desired volume), then reduce the regulated pressure to the bottle. (If Dispensed Volume is low, increase the pressure; 1 psi is approximately 10% volume change.)

12. If the pressure changed, repeat the Verification step. Keep changing the pressure and verifying until the Dispensed Volume matches the Requested Volume Setting within your acceptable error.

13. Exit the ‘Liquid Profile Preparation’ window and enter the final pressure into the Profile Qualification Information settings. These are notes to record the optimum pressure at three Volume/Pressure settings. They have no active function.

Chapter 4

Instrument Qualification

This chapter contains procedures for qualifying the initial and ongoing performance of the NanoQuot™.

Overview................................................................................54 IQ/OQ/PQ...............................................................................54 Qualification Schedule ..............................................................56 Dispense Accuracy and Precision Tests........................................57 Specifications .....................................................................57 Required Materials...............................................................58 Test Solution Recipes...........................................................59 Equipment Setup ................................................................59 Dispense Accuracy Test Procedure .........................................60 Dispense Precision Test Procedure .........................................62 Dispense Accuracy & Precision Worksheet: 0.10 µl Dispense Dispense Accuracy & Precision Worksheet: 1.0 µl Dispense

54 | Chapter 4: Instrument Qualification


Overview

Every NanoQuot™ is fully tested at BioTek prior to shipment and should operate properly upon initial setup. If you suspect that a problem occurred during shipment, if you have received the equipment after returning it to the factory for service, and/or if regulatory requirements dictate that you qualify the equipment on a routine basis, you should perform the procedures outlined in this chapter.

This chapter contains BioTek Instruments’ recommended Installation Qualification (IQ), Operational Qualification (OQ), and Performance Qualification (PQ) procedures for the NanoQuot Microplate Dispenser.

A Product Qualification Package (PN 7150503) for the NanoQuot is available for purchase. The package contains complete procedures for performing Installation Qualification, Operational Qualification, Performance Qualification, and Preventive Maintenance procedures. Extensive Checklists and Logbooks are provided for recording results. Contact your local BioTek dealer for more information.

IQ/OQ/PQ

Installation Qualification confirms that the instrument and its components have been supplied as ordered and ensures that they are assembled and configured properly for your lab environment.

• The recommended IQ procedure consists of setting up the NanoQuot and its components as described in Chapter 2, Installation, and performing the Self-Test. A quick test of the fluidics system is also performed, to ensure that the tubing is properly installed and there are no leaks.

• The IQ procedure should be performed initially (before the instrument is used for the first time).

• The successful completion of the IQ procedure confirms that the instrument and its components are installed correctly. The Operational Qualification should be performed immediately following the successful IQ.

IQ/OQ/PQ | 55


Operational Qualification confirms that the instrument operates according to specification initially and over time.

• The recommended OQ procedure consists of performing the Dispense Accuracy and Precision Tests.

• Your facility’s operating policies may also require that you perform an actual assay prior to accepting the instrument for routine use. If this is the case, you should not use the data obtained from the first assay run with the instrument until you have confirmed that the package insert criteria have been met.

• The OQ procedure should be performed initially (before first use) and then routinely; the recommended interval is annually. It should also be performed after any major repair or upgrade to the hardware or software.

• Results should be compared with those from the routine Performance Qualification tests and previous OQ tests to monitor for trends.

• Although out-of-tolerance failures will be detected by the OQ tests, results should be compared with those from the routine Performance Qualification tests and previous OQ tests to monitor for trends.

• The successful completion of the OQ procedure, in combination with results that are comparable to previous PQ and OQ tests, confirms that the instrument is operating according to specification initially and over time.

Performance Qualification confirms that the instrument consistently meets the requirements of the tests performed at your laboratory.

• The recommended PQ procedure consists of performing the Dispense Accuracy and Precision Tests.

• Your facility’s operating policies may also require that you routinely perform an actual assay, to confirm that the instrument will consistently give adequate results for the assays to be run with it.

• These tests should be performed routinely; the recommended interval is monthly or quarterly, depending on the test. This frequency may be adjusted depending on the trends observed over time.

• The successful completion of the PQ procedure confirms that the instrument is performing consistently under normal operating conditions.



Qualification Schedule

The schedule below defines the factory-recommended intervals for qualifying a NanoQuot™ used two to five days a week. The actual frequency, however, may be adjusted depending on your usage of the instrument. This schedule assumes the dispenser is properly maintained as outlined in Chapter 5, Preventive Maintenance.

IQ OQ PQ Tasks/Tests

Initially Initially & Annually

Monthly Quarterly

Installation and Setup:

NanoQuot Dispenser and its components

Host Computer (if required)

NanoQuot PC Control Software (if required)

Power-Up System Test

Fluidic System Test

Dispense Accuracy and Precision Tests

‘Run Assay’ Test (optional)

Dispense Accuracy and Precision Tests | 57


Dispense Accuracy and Precision Tests

BioTek Instruments, Inc. has developed a set of tests that you can perform to ensure that the dispenser performs to specification initially and over time. We recommend that you perform these tests before first use (after the IQ), and then every three months.

• The Accuracy Test is a measure of the average volume dispensed per well. It is independent of precision. The volume per well may vary over a plate, yet the accuracy may be exact because it is an average of the volumes dispensed per well. When testing accuracy, a program is run that dispenses the selected volume (x replicates) into a new, 96-well strip. The strip is weighed before and after dispensing.

• The Precision Test is a measure of the variability of volumes dispensed from tip to tip across the manifold. The optical density of the solution in a well is proportional to the total volume of the solution in the well. If the % Coefficient of Variation (%CV) is calculated, the result is a measure of the uniformity of the distribution of dispensed volumes across the manifold. It is the ratio, expressed in percent, of the standard deviation of the distribution of fluid volumes in the wells to the mean value of volume per well. Precision is checked in this test. The %CV is lowered as the uniformity of distribution across the plate improves.

When testing precision, dye solution is dispensed to a new, 384-well microplate. Testing is done by dispensing 1.0 µl and 0.10 µl per well. 80 µl of filtered water is added to each well before reading, using a hand pipette or other calibrated dispensing tool. The contents of the plate are then shaken on a microplate shaker or in a microplate reader before reading. The average absorbance of the dye solution is between 0.700 and 1.300 OD when read on a microplate reader using an appropriate wavelength.

Specifications

Dispense Accuracy Dispense Precision

± 7% at volumes ≥ 1 µl < 7% CV at volumes ≥ 1 µl

± 12% at 500 nl < 12% CV at 500 nl

± 12% at 100 nl < 12% CV at 100 nl



Required Materials

• Absorbance microplate reader capable of dual wavelength reading at 630/450 nm or 630/405 nm. The reader must support 384-well plates.

• Microplate shaker (if the absorbance reader does not support plate shaking)

• Precision balance with resolution of 0.001 g

• Hand pipette and disposable tips

• (Optional) bulk reagent dispenser or automated pipettor capable of dispensing to 384-well plates

• Clean, filtered water (at least 100 ml)

• BioTek NanoQuot Blue Test Dye, 125 ml (PN 7773004) or FD&C #1 Blue Dye powder

BioTek’s NanoQuot Blue Test Dye contains FD&C Blue #1 and Sodium Azide N3Na as a preservative.

• The Final Acceptance data sheet that came with your NanoQuot (contact BioTek TAC for a copy if necessary)

• 100 ml glass supply bottle

• 100 ml graduated cylinder

• Clean, dry 96-well format 1x8 microstrip and microstrip holder

• Clean, dry 384-well clear-bottom microplate

• Data reduction software (either on a computer or in the absorbance reader) that can be configured to calculate the mean, standard deviation, and %CV

• (Optional) Worksheets at the end of this chapter



Test Solution Recipes

Important! Use clean, filtered water in the following solutions.

• The Test Solution should contain water and dye.

• The absorbance of the blue dye solutions should be measured at 630 nm and 450 nm (or 405) reference.

• Add enough colored dye to the solution to produce an absorbance between 0.700 and 1.300 OD when dispensing 1.0 µl or 0.10 µl into the microwell and diluting it with 80 µl of water. The recipe will vary, depending on dye strength.

• If you are using FD&C #1 Blue Dye, mix 0.75 g of the powder in 100 ml of clean, filtered water. Mix until the powder is completely dissolved. Verify that the solution produces acceptable OD readings.

• The BioTek NanoQuot Blue Test Dye, 125 ml (PN 7773004) is ideal for this application. If using the Test Dye:

0.10 μl Test: Use the dye as-is; no dilution is required.

1.0 μl Test Solution: Mix 90 ml of clean, filtered water with 10 ml of the Blue Test Dye. Verify that the solution produces acceptable OD readings.

Contact BioTek’s Technical Assistance Center if further instructions are necessary. Refer to Chapter 1 for contact information.

Equipment Setup

For both tests:

• Set up the precision balance according to the manufacturer’s instructions. For the Dispense Precision Test:

• Set up the absorbance microplate reader to read 384-well plates at 630/450 (or 405) nm.

• Set up the bulk reagent dispenser to dispense 80 μl per well of water to 384-well plates, per the manufacturer’s instructions.



Dispense Accuracy Test Procedure

Perform this procedure twice: once at 0.10 µl, then again at 1.0 µl.

This procedure can be performed manually using the keypad and thumbwheels, or from the PC Control Software using the Prime button and the Program File Management dialog.

Turn the pressure to the supply bottle OFF before removing the cap to fill the bottle. Turn the pressure back ON only after the bottle is tightly secured.

1. Select the Liquid Profile that corresponds with the test solution.

2. If using BioTek’s NanoQuot Blue Test Dye, or a dye solution created using FD&C #1 powder:

• Select the Liquid Profile for DI water @ 12.5 psi.

• Locate the Final Acceptance data sheet that shipped with the NanoQuot. In the “Volume Accuracy Verification” section, three Pressure values were recorded at the factory, one per test volume. These values are specific to the instrument serial number recorded at the top of the data sheet, using BioTek’s NanoQuot Blue Test Dye.

• Set the supply bottle pressure to match the appropriate Pressure value recorded on the data sheet.

3. If the system is dry and/or air bubbles are present in the tubing, Purge the Dispenser to eliminate any air bubbles from the system.

4. Fill the supply bottle with clean, filtered water. Prime the dispenser with 10 µl Volume with 50 Repetitions (set Cols to 50, if using manual mode). Verify that the water has filled the tubing completely. Repeat the prime if necessary.

Exercise caution when transferring tubing between bottles. Keep the tube pointed down to prevent an air bubble from entering the tube. If an air bubble does enter the tubing, the NanoQuot should be repurged.

5. Release the bottle clamp and then loosen the bottle cap. Very carefully remove the cap with tubing from the bottle.

6. Replace the water in the supply bottle with the test solution and then tighten the cap.

7. Prime the dispenser with 10 µl Volume with 50 Repetitions (set Cols to 50, if using manual mode). This will utilize 4 ml of liquid (10 µl x 50 repetitions x 8 tips = 4 ml).



8. Verify that the test solution has filled the tubing completely. Repeat the prime if necessary.

9. Weigh a clean, dry 96-well format 1x8 strip; this is the Initial Weight.

10. Place the strip into position 1 in the microstrip holder and then place the holder on the dispenser’s microplate carrier.

11. Set the test Volume (Volume thumbwheels) and # of Replicates (Cols thumbwheels) for the Dispense procedure. We recommend that for 1.0 µl, the replicates are set to 50, and for 0.10 µl, the replicates are set to 99. Specify 96-well plate format, Manual mode.

12. Press the Cal key (or click Dispense in the PC Control Software). Fluid will dispense into all wells of the strip.

13. Weigh the strip again and subtract its Initial Weight. Record the Actual Weight (mass) in the worksheet at the end of this chapter.

14. Calculate the Expected Mass and record it in the worksheet.

The Expected Mass of the dispensed fluid is the volume in milliliters times the number of replicates dispensed times the number of tips dispensing. For example, if 1 µl is dispensed with 50 replicates through 8 tips, then the expected mass is 0.001 x 50 x 8 = 0.400 grams.

15. Calculate the % Accuracy Error and record it in the worksheet.

Subtract the Expected mass from the Actual mass dispensed, and divide the result by the Expected mass. Multiply this value by 100 to determine the % Accuracy Error. Example:

Actual Mass = 0.410 grams (1 µl dispense) Expected Mass = 0.400 grams = (0.001 grams x 50 replicates x 8 tips) % Accuracy Error = (0.410 – 0.400) / 0.400 = 0.025 x 100 = 2.5% = PASS

• If the result is greater than the Accuracy specification, perform another Purge and then repeat the test.

• If using a stock Liquid Profile, or if better accuracy is required, adjust the pressure to the supply bottle. Note that increases in pressure will result in larger dispense volumes.

Dispense Accuracy Specifications. When dispensing water with FD&C #1 blue dye, at dual wavelength, using wavelengths appropriate for the dye:

± 7% at volumes ≥ 1 µl ± 12% at 500 nl ± 12% at 100 nl



Dispense Precision Test Procedure

Perform this procedure twice: once at 0.10 µl, then again at 1.0 µl.

Turn the pressure to the supply bottle OFF before removing the cap to fill the bottle. Turn the pressure back ON only after the bottle is tightly secured.

1. Select the Liquid Profile that corresponds with the test solution. If using BioTek’s NanoQuot Blue Test Dye, or a dye solution created using FD&C #1, select the Liquid Profile for DI water @ 12.5 psi.

2. Dispense the required test volume into 24 columns of a clean, dry 384-well microplate. Visually verify that the droplets are dispensed into the wells.

3. Add 80 µl of water to the dye solution in the wells using a calibrated hand pipette or other automated pipettor.

4. Shake the contents of the plate on a microplate shaker or in a microplate reader for 60 seconds. This will result in a uniformly mixed solution and create a more uniform meniscus in each of the wells. Note any obvious error-causing formations (large bubbles, foreign matter, etc.).

5. Read the plate in an absorbance microplate reader using the dual-wavelength method, to reduce the influence of scratches and foreign particles that could be in the well. Print the results.

6. Calculate the Mean Absorbance, Standard Deviation, and the %CV (% Coefficient of Variation) for the wells being tested. Record the information in the appropriate worksheet at the end of this chapter.

Example:

Mean Absorbance = 1.200 OD Standard Deviation = 0.040 % CV = SD / Mean * 100 = 3.3% = PASS

• The %CV must be < 12% to Pass for the 0.10 µl/384-well test.

• The %CV must be < 7% to Pass for the 1.0 µl/384-well test.

If your result fails these criteria, Purge the dispenser and retest. If you notice that one or more columns in the plate are not in compliance, use the Valve Bias Settings in the NanoQuot™ PC Control Software to adjust the bias. Refer to the help system in the PC Control Software for instructions.

Dispense Precision Specifications. When dispensing water with FD&C #1 blue dye, at dual wavelength, using wavelengths appropriate for the dye:

< 7% CV at volumes ≥ 1 µl < 12% CV at 500 nl < 12% CV at 100 nl

© 2006, BioTek Instruments, Inc.

Dispense Accuracy & Precision Worksheet: 0.10 µl Dispense

0.10 µl Dispense Accuracy Test

Record the pressure to the supply bottle: psi

Record the liquid profile used:

Total Dispense Weight (Actual Mass): grams

Expected Mass: (0.0001 g/well x number of replicates x number of tips)

grams

% Accuracy Error: (Actual Mass – Expected Mass) ÷ Expected Mass x 100

%

% Accuracy Error must be ± 12% Pass Fail

0.10 µl Dispense Precision Test


Mean Absorbance:

Standard Deviation (SD):

% CV (SD ÷ Mean x 100): %

% CV must be < 12% Pass Fail

NanoQuot Serial Number:

Tests Performed By:

Date:

Reviewed/Approved By:

Date:

© 2006, BioTek Instruments, Inc.

Dispense Accuracy & Precision Worksheet: 1.0 µl Dispense

1.0 µl Dispense Accuracy Test

Record the pressure to the supply bottle: psi


Total Dispense Weight (Actual Mass): grams

Expected Mass: (0.001 g/well x number of replicates x number of tips)

grams

% Accuracy Error: (Actual Mass – Expected Mass) ÷ Expected Mass x 100

%

% Accuracy Error must be ± 7% Pass Fail

1.0 µl Dispense Precision Test


Mean Absorbance:

Standard Deviation (SD):

% CV (SD ÷ Mean x 100): %

% CV must be < 7% Pass Fail

NanoQuot Serial Number:

Tests Performed By:

Date:

Reviewed/Approved By:

Date:

Chapter 5

Preventive Maintenance

This chapter describes how to maintain, decontaminate, and store the NanoQuot™.

Recommended Maintenance Schedule.........................................66 Warnings and Precautions .........................................................68 Tools and Supplies ...................................................................69 Rinse the Fluid Path .................................................................70 Clean Components...................................................................72 Decontamination .....................................................................77 Replace Components................................................................80 Prepare for Storage or Shipment................................................86 Maintenance Logs ....................................................................87

66 | Chapter 5: Preventive Maintenance


Recommended Maintenance Schedule

Overview

Preventive Maintenance (PM) represents a set of procedures that should be performed regularly to maintain equipment in top condition, maximize running time, and extend the life of the dispenser. A routine maintenance schedule includes:

• Rinsing the fluid path

• Cleaning components

• Decontaminating the external surfaces and fluid path

• Replacing components as needed For proper care of the NanoQuot™, BioTek created three maintenance programs that may be run during either manual or PC control of the dispenser: Purge, Prime, and Clean. The following table compares these three programs:

Program Suggested Use Settings Recommendations

Purge At initial setup and when air gets into tubing; also recommended for daily maintenance

System-defined settings; 18 minute run time

Set Repetitions/Cols to 9 to purge all valves, or 1-8 to purge valves A-H individually.

Prime Prior to dispensing, and after a long delay, to fill tubing with reagent; for daily maintenance

User-defined settings; volume limited by Liquid Profile

Set Volume and Repetitions greater than or equal to 2 ml to replace fluid in tubing

Clean For flushing tubing with water, detergent, etc.; for daily maintenance and Decontamination

User-defined repetitions, system-defined volume

Set Repetitions to 10 to completely flush system

To run a Clean program during manual control of the NanoQuot, run a Prime with the Volume set higher than the current Liquid Profile (e.g., 99.99). See Manual Control of the NanoQuot in Chapter 3.

Recommended Maintenance Schedule | 67


Schedule

The following chart lists maintenance tasks and the frequency with which each task should be performed. Maintenance Logs are provided at the end of this chapter.

It is important to note that the risk and performance factors associated with your assays may require performing some or all of the procedures more frequently than presented in the schedule.

Frequency

Tasks Page Daily Monthly

Before Storage/ Shipment

As Needed

Rinse the Fluid Path

Run Purge (optional, but recommended) and Prime at the start of the day

70

Run Clean at the end of the day

71

Remove protein residuals 71 weekly

Clean Components

Clean bottles and tubes 72

Clean fluidics cover 72

Clean dispense head 73

Clean plate carrier system 75

Clean priming trough 76

Clean compressor 76

Decontaminate the Dispenser

Decontaminate external surfaces

78

Decontaminate fluid path 79

Replace Components

Replace dispense valves and tubing

80

Replace fluid path assembly 84



Warnings and Precautions

Please read the following before performing any maintenance procedures.

Warning! Internal Voltage. Turn off and disconnect the NanoQuot™ from its power supply for all cleaning and decontamination operations.

Warning! Wear prophylactic gloves when handling contaminated instruments. Gloved hands should be considered contaminated at all times; keep gloved hands away from eyes, mouth, nose, and ears. Eating and drinking while decontaminating instruments is not advised.

Warning! Mucous membranes are considered prime entry routes for infectious agents. Wear eye protection and a surgical mask when there is a possibility of aerosol contamination. Intact skin is generally considered an effective barrier against infectious organisms; however, small abrasions and cuts may not always be visible. Wear protective gloves when handling contaminated instruments.

Warning! Do not let air follow fluid into the supply tubing during an operation. This will cause the fluid to be emitted as an aerosol. Depending on the fluid, this could be hazardous to your health.

Warning! Do not immerse the instrument, spray it with liquid, or use a “wet” cloth. Do not allow water or other cleaning solution to run into the interior of the instrument. If this happens, contact BioTek’s Technical Assistance Center.

Tools and Supplies | 69


Caution! Some chemicals may cause irreparable damage to this instrument. The following chemicals have been deemed safe for use in this instrument: buffer solutions (such as PBS), saline, surfactants, filtered deionized or distilled water, 70% ethyl, isopropyl, or methyl alcohol, 40% formaldehyde, and 20% sodium hydroxide. Other chemicals may cause severe damage to the instrument. Contact BioTek Instruments if you require further assistance. Refer to Appendix B, Chemical Compatibility. Do not expose any part of the instrument to sodium hypochlorite solution (bleach) for more than 20 minutes. Prolonged contact may damage the instrument surfaces. Be certain to rinse and thoroughly wipe all surfaces.

Caution! Do not soak the keypad, thumbwheels, or rocker switches—this will cause damage. Wipe with a damp cloth.

Tools and Supplies

• Dish soap or other mild cleaner

• Clean, filtered water

• 125 ml beakers

• 0.5% sodium hypochlorite or 70% isopropyl alcohol

• Surgical mask

• Safety glasses

• Protective gloves

• Lab coat

• Biohazard trash bags

• Biohazard container

• Clean, lint-free cotton cloths, or disposable towels

• 1/4” slotted (straight) screwdriver

• 3/32” hex wrench

• Phillips-head screwdriver

• Small piece of sandpaper (when replacing valves)

• Spare 100 ml supply bottles (when preparing for long-term storage or shipment)



Rinse the Fluid Path

Important! When changing fluids, try not to let air into the tubing. Leave a droplet of fluid at the end of the tube in the supply bottle; do not shake it off. Do not let the supply bottle empty during an operation. Do not let air follow fluid into the tubing during an operation.

Important! When rinsing the fluid path with deionized water, fluid may collect on the tips. If this is a problem, distilled water could be used instead.

For all daily maintenance programs, ensure that the supply bottle contains at least 100 ml of rinse solution, and that the prime trough and waste bottle are empty.

Purge and Prime at the Start of the Day

Daily maintenance involves flushing the dispenser with an appropriate reagent at the beginning and end of the day. (BioTek recommends using clean, filtered water.) The Purge and Prime programs are recommended for the start of the day.

1. Turn the pressure to the supply bottle Off.

2. Fill the bottle with clean, filtered water and tighten the cap.

3. Turn the pressure to the supply bottle On.

4. Run the Purge program. This program runs for approximately 18 minutes and will remove all air from the system. It pushes fluid through the tubing at several different frequencies, to ensure air bubbles of various sizes are eliminated.

5. Replace the water with the desired reagent, being careful not to introduce air into the pick-up at the bottom of the tubing.

6. Perform a Prime sufficient to clear the lines of the water. The dispenser is now ready to run a Dispense program.

This should be done on the same day the microplates are dispensed. This procedure helps prevent the dispense tubes from clogging between uses.

BioTek recommends running the Prime program periodically if the dispenser is used intermittently throughout the day.

Rinse the Fluid Path | 71


Clean at the End of the Day

Daily maintenance involves flushing the dispenser with an appropriate reagent at the beginning and end of the day (BioTek recommends using clean, filtered water). The Clean program is recommended for the end of the day.


2. Replace the reagent with clean, filtered water, being careful not to introduce air into the pick-up at the bottom of the tubing in the supply bottle.


4. Perform a Clean sufficient to clear the lines of the reagent.

5. Leave the water in the dispense tubing overnight.

Remove Protein Residuals and Fungi Growth

Important! Solutions containing proteins, such as bovine serum albumin (BSA), will compromise the dispenser’s performance over time unless a strict maintenance regime is adhered to. Do not use ethanol or isopropyl alcohol to flush out BSA.

BioTek recommends performing the following maintenance procedure to thoroughly flush out protein particles and other contaminants from the dispenser’s fluid path. The recommended frequency is weekly.

1. Run a Prime to flush the system with 0.1 – 0.5 N NaOH, followed by neutralization with an equivalent normality (0.1 – 0.5 N) of HCl.

2. Rinse well with clean, filtered water to remove the HCl.

3. Run a Clean program with filtered water, or run a Prime at least three times with filtered water if you plan to use the Dispenser immediately.

To completely replace fluid in the tubing, set the Volume and Repetitions combination to be greater than or equal to 2 ml.



Clean Components

Warning! Turn off and unplug the instrument for all cleaning operations. Do not immerse the instrument, spray it with liquid, or use a “wet” cloth. Do not allow the cleaning solution to run into the interior of the instrument. If this happens, contact the BioTek Service Department.

Clean the Bottles and Tubes

• To prevent bacteria growth, clean all bottles and tubes with filtered water before the first use, before each refill, and if they have been idle for any length of time.

• Rinse the bottle caps every time the dispense or rinse bottles are filled.

• Accumulated algae, fungi, or mold may require decontamination. Decontamination is described on page 77.

Clean the Fluidics Cover

To clean the fluidics cover:

1. Turn off and unplug the instrument.

2. Moisten a lint-free cotton cloth with water, or water and mild detergent. Do not soak the cloth.

3. Remove the fluidics cover (see instructions and photos under “Remove Shipping Hardware” in Chapter 2).

4. Wipe the inside and outside surfaces of the fluidics cover with the cloth.

5. Wipe the top surface of the dispenser’s base. Wipe all exposed surfaces of the instrument.

6. If detergent was used, wipe all surfaces with a cloth moistened with water.

7. Use a clean, dry cloth to dry all wet surfaces. Keep the cover off for the next maintenance procedure.

Clean Components | 73


Clean the Dispense Head

Important! The dispense head is not autoclavable.

Regular rinsing helps to keep the dispense head and dispense tips clean. Follow the Decontamination procedure on page 79 to disinfect the manifold and tubing.

If you suspect a particular problem is related to the dispense head (for example, air bubbles can result in uneven dispensing), try running a Purge first. If this is not effective, perform a thorough cleaning of the manifold.

To clean the dispense head:

1. If you have not already done so:

• Turn off and unplug the NanoQuot.

• Remove the fluidics cover (see page 72).


3. Disconnect the tubing between the supply bottle and the distribution manifold (Figure 13).

4. Use the “L” shaped 3/32” hex wrench to remove the setscrew on the front of the dispense head (Figure 14).

5. Pull the dispense head off the alignment pins.

6. Using a lint-free disposable towel or swab, thoroughly clean the underside of the dispense head, being careful not to damage the tips (Figure 15).

7. Set the dispense head back on the alignment pins and replace the set screw.

8. Replace the fluidics cover, being careful not to crimp the tubes on the distribution manifold, by guiding the tubes into the lip on the inside the cover. Tighten the thumbscrew. Tip: Use a tie wrap to temporarily bundle the tubes to keep them from kinking/crimping.

9. Reconnect the tubing between the supply bottle and the distribution manifold.

10. Plug in and turn on the NanoQuot.


12. Run a Purge using clean, filtered water.



Figure 13: Disconnect the supply tube fitting from the distribution manifold

Figure 14: Remove the setscrew on the front of the dispense head

Dispense Head

3/32” hex wrench

Supply tube fitting

Distribution manifold

Clean Components | 75


Figure 15: Clean the underside of the dispense head; do not damage the tips!

Clean the Plate Carrier System

If liquid has overflowed onto the plate carrier, some crystal buildup may occur and prevent the plate carrier from moving correctly.

1. Clean the carrier:

• Move the carrier to its ”home” position (turn the dispenser on, wait for the Self-Test to complete, then turn the dispenser off).

• Clean the carrier using soap and water or 70% isopropyl alcohol.

2. Wipe the rails:

• Using a lint-free wipe or swab, wipe the rails clean, moving the carrier as required for clearance. Do not lubricate the rails!

Note: Each carrier is specifically aligned to each Dispenser. Do not adjust the screws that fasten the carrier to the rails. Do not interchange the carrier with one from another instrument.



Clean the Priming Trough

Clean the priming trough regularly to prevent bacteria growth and residue buildup, by running a Prime several times with reagent or water to rinse the trough. Alternatively, pour the fluid from a beaker directly into the trough to fill it, and then allow it to drain.

Clean/Maintain the Compressor

Regular maintenance of the air compressor is necessary to ensure optimum pump performance. Clean/Maintain the compressor according to the procedures provided in the compressor’s instruction manual.

Decontamination | 77


Decontamination

Any laboratory instrument that has been used for research or clinical analysis is considered a biohazard and requires Decontamination prior to handling. Decontamination minimizes the risk to all who come into contact with the instrument during shipping, handling, and servicing. Decontamination is required by the U.S. Department of Transportation regulations.

Intact skin is generally considered an effective barrier against infectious organisms; however, small abrasions and cuts may not always be visible.

Persons performing the decontamination process must be familiar with the basic setup and operation of the instrument.

Important! BioTek Instruments, Inc. recommends the use of the following decontamination solutions and methods based on our knowledge of the instrument and recommendations of the Centers for Disease Control and Prevention (CDC). Neither BioTek nor the CDC assumes any liability for the adequacy of these solutions and methods. Each laboratory must ensure that decontamination procedures are adequate for the Biohazard(s) they handle.

Warning! Internal Voltage. Turn off and disconnect the NanoQuot™ from its power supply for all decontamination operations.

Warning! Wear prophylactic gloves when handling contaminated instruments. Keep gloved hands away from eyes, mouth, nose, and ears. Eating and drinking while decontaminating instruments is not advised.

Warning! Mucous membranes are considered prime entry routes for infectious agents. Wear eye protection and a surgical mask when there is a possibility of aerosol contamination.



Decontaminate External Surfaces

Warning! The bleach solution is caustic; wear gloves and eye protection when handling this solution. Do not soak the keypad, thumbwheels, or rocker switches—this will cause damage. Wipe them with a cloth moistened with the bleach solution or alcohol.

Caution! Be sure to check the percent NaClO of the bleach you are using; this information is printed on the side of the bottle. Commercial bleach is typically 10% NaClO; if this is the case, prepare a 1:20 dilution. Household bleach is typically 5% NaClO; if this is the case, prepare a 1:10 dilution.

1. Turn the instrument off and disconnect the power supply.

2. Prepare an aqueous solution of 0.5% sodium hypochlorite (NaClO, or bleach). As an alternative, 70% isopropyl alcohol may be used if the effects of bleach are a concern.

3. Moisten a cloth with the bleach solution or alcohol. Do not soak the cloth.

• Wipe the keypad, thumbwheels, and switches (do not soak). Wipe again with a clean cloth moistened with filtered or distilled water. Dry immediately with a clean, dry cloth.

• Remove the fluidics cover (see page 72). Wipe the inside and outside surfaces of the fluidics cover. Wipe the plate carrier, priming trough, top surface of the instrument’s base, supply bottles, bottle covers, tubing, and all exposed surfaces of the instrument.

4. Wait 20 minutes. Moisten a cloth with filtered or distilled water.

• Wipe the inside and outside surfaces of the fluidics cover. Wipe the plate carrier, priming trough, top surface of the instrument’s base, supply bottles, tubing, bottle covers, and all exposed surfaces of the instrument that have been cleaned with the bleach solution or alcohol.

5. Use a clean, dry cloth to dry all wet surfaces.

6. Reassemble the instrument.

7. Discard used gloves and cloths using a Biohazard trash bag and an approved Biohazard container.

Decontamination | 79


Decontaminate the Fluid Path

Important! When decontaminating the fluid path with deionized water, fluid may collect on the tips. If this is a problem, distilled water could be used instead.

Important! When changing fluids, try not to let air into the tubing. Leave a droplet of fluid at the end of the tube in the bottle; do not shake it off. Do not let the supply bottle run empty during an operation. Do not let air follow fluid into the tubing during an operation.

Caution! Be sure to check the percent NaClO of the bleach you are using; this information is printed on the side of the bottle. Commercial bleach is typically 10% NaClO; if this is the case, prepare a 1:20 dilution. Household bleach is typically 5% NaClO; if this is the case, prepare a 1:10 dilution.

Run a Clean program to decontaminate the internal tubing and manifold parts:

1. If you have not already done so, perform the decontamination procedure described on the previous page.

2. Prepare an aqueous solution of 0.5% sodium hypochlorite (NaClO, or bleach).

3. Fill the supply bottle with 100 ml of bleach solution (disinfectant).

4. Run a Clean program.

5. Replace the bleach solution in the bottle with 100 ml of clean, filtered water (rinse). Do not allow the bleach solution to soak inside the tubing.

6. Run a Clean program at least two times to flush out all bleach.



Replace Components

Replace a Solenoid Valve Assembly

Important! After replacing a valve asssembly, you will need to run a Purge and a Prime, and then perform the Dispense Accuracy and Precision Tests to verify performance.

Important! Do not overtighten the setscrews on the valves, or you may crush the valves!

If a dispense valve leaks or is stuck open or closed, the valve and its tubing should be replaced. The NanoQuot comes with one spare valve clipped to the front of the instrument under the fluidics cover. A spare tube with the fitting attached is also supplied. To order additional valve/tubing sets, contact BioTek Customer Care and ask for part number 76068.

Figure 16: Replacement solenoid valve assembly, that includes valve and tubing to distribution manifold with 1/8” fitting

To replace a dispense valve and its tubing:

1. Turn the pressure to the bottle OFF.

2. Turn off and unplug the NanoQuot.

Valve

1/8” fitting to distribution manifold

Replace Components | 81


3. Remove the fluidics cover (see instructions under “Remove Shipping Hardware” in Chapter 2).

4. Disconnect the tubing between the supply bottle and the distribution manifold (Figure 13 on page 74).

5. For the valve assembly that is being replaced:

• Disconnect the assembly’s fitting from the distribution manifold (Figure 17).

• Carefully pull the cable connector off the valve (Figure 18).

• Using a 0.050” hex wrench, loosen the set screw (see Figure 19) that holds the valve in place, and then slowly pull the valve up and out of the dispense head (Figure 20).

• Set the old valve assembly aside.

Note: The NanoQuot comes with one spare valve clipped to the front of the instrument under the fluidics cover. Contact BioTek to order additional spare valves.

7. To install the new valve and tubing:

• Push the “flared” (open) end of the tubing onto the valve’s barb. Grip the tubing with a small piece of sandpaper if necessary (Figure 21).

• Insert the new valve into the dispense head and then tighten the set screw. Do not overtighten!

• Replace the cable connector.

• Attach the fitting on the other end of the tube to the distribution manifold.

6. Replace the fluidics cover, being careful not to crimp the tubes on the distribution manifold. Guide the tubes into the lip on the inside the cover. Tip: Use a tie wrap to temporarily bundle the tubes to keep them from crimping.

7. Tighten the thumbscrew that attaches the distribution manifold to the inside of the fluidics cover.

8. Reconnect the tubing between the supply bottle and the distribution manifold.

9. Plug in and turn on the NanoQuot. Turn the pressure to the bottle ON.

10. Prepare the new valve assembly by running a Purge three times using clean, filtered water. Follow the Purge with at least one Prime. If there is a droplet on the valve tip after the Purge and Prime, gently wipe it off with a clean, lint-free cloth.

11. Perform the Dispense Accuracy and Precision Tests in Chapter 4, Instrument Qualification to test the valve performance.



Figure 17: Disconnect the valve assembly’s fitting from the distribution manifold

Figure 18: Remove the cable connector from the valve

Cable connector (1 of 8)

Valve to be replaced



Figure 19: Loosen the valve’s set screw

Figure 20: Pull the valve out of the dispense head

Valve

Tubing

Valve set screw (1 of 8)



Figure 21: Slide the flared end of the tubing over the valve’s “barb”. Use a small piece of sandpaper to grip the tube, if necessary.

Replace the Fluid Path Assembly

A replacement “fluid path assembly” is available from BioTek. It includes a dispense head, 8 solenoid valves, 8 tubes, distribution manifold, and supply tubing, completely assembled.

To remove the existing fluid path assembly:


2. Turn off and unplug the NanoQuot.

3. Remove the fluidics cover (see page 72).

4. Disconnect and remove the tube from the supply bottle.

5. Carefully remove all eight cable connectors from the valves (Figure 18).

6. Remove the set screw that holds the dispense head in place and then slide the dispense head off its alignment pins (Figure 14).

7. Remove the entire fluidic path assembly and set it aside.

To install the new fluid path assembly:

1. Set the dispense head on the alignment pins and replace the set screw.

2. Replace the cable connectors in their lettered order, starting with “A” which is closest to the rear of the instrument (Figure 22).

3. Connect the supply tube to the bottle and tighten the fitting.



4. Replace the fluidics cover, being careful not to crimp the tubes on the distribution manifold. Guide the tubes into the lip on the inside the cover.

Tip: Use a tie wrap to temporarily bundle the tubes to keep them from crimping.

5. Tighten the thumbscrew that attaches the distribution manifold to the inside of the fluidics cover.

6. Fill the supply bottle with clean, filtered water and then run a Purge, followed by a Prime.

8. Prepare the new valves/tubing by running a Purge three times using clean, filtered water. Watch the fluid flow from each valve to verify that the tubing from the distribution manifold is properly connected to the valves. Follow the Purge with at least one Prime. If there are droplets on the valve tips after the Purge and Prime, gently wipe them off with a clean, lint-free cloth.

9. Perform the Dispense Accuracy and Precision Tests in Chapter 4, Instrument Qualification to test performance.

Figure 22: Replace all eight cable connectors

Cable connectors (8 total) H

A



Prepare for Storage or Shipment

Remove Liquid from the Fluid Path

Warning! Be aware that, as fluid leaves the system, it may “aerosol.” If any harmful reagents have been used, do not allow the dispenser to “aerosol.”

Prepare one supply bottle with at least 100 ml of disinfectant (70% isopropanol) and one with at least 100 ml of rinse (clean, filtered water). You also need one empty supply bottle (this is the “air” bottle).

When using deionized water, fluid may collect on the tips. If this is a problem, distilled water could be used instead.

Before leaving the instrument unused for a long period of time, perform these steps:

1. Flush the supply tubing and manifold repeatedly with disinfectant.

2. Flush the system with clean, filtered water.

3. Purge the system of fluid by attaching a bottle with no fluid (pressurized air only), and pressing the Prime button until the remaining fluid in the lines is gone.

Store the Dispenser in an Appropriate Environment

After removing liquid from the instrument’s fluid path, turn off the dispenser and disconnect it from its power supply.

Store the dispenser in a dust- and particle-free environment, and protect it from temperature extremes that can cause condensation within the instrument and from corrosive fumes and vapors.

Store the dispenser under the following environmental conditions:

Temperature: 5° to 40°C (41° to 104°F)

Relative humidity: 10% to 85% (non-condensing)

Maintenance Logs | 87


Maintenance Logs

Daily and monthly maintenance logs are provided on the following pages.

Nan

oQ

uo

t S

eri

al N

um

ber:

Date

DA

ILY

M

AIN

TEN

AN

CE ~

Ch

eck

()

wh

en

task

is

com

ple

ted

Rin

se t

he F

luid

Path

Pu

rge a

nd P

rim

e a

t

the

star

t of

the

day

Cle

an

at

the

end

of

the

day

Op

era

tor’

s In

itia

ls

Date

DA

ILY

M

AIN

TEN

AN

CE ~

Ch

eck

()

wh

en

task

is

com

ple

ted

Rin

se t

he F

luid

Path

Pu

rge a

nd P

rim

e a

t

the

star

t of

the

day

Cle

an

at

the

end

of

the

day

Op

era

tor’

s In

itia

ls

Nan

oQ

uo

t Seria

l Nu

mb

er:

Date

MO

NTH

LY

/A

S N

EED

ED

MA

INTEN

AN

CE ~

Ch

eck

() w

hen

task

is com

ple

ted

Rin

se th

e F

luid

Path

Rem

ove Pro

tein R

esiduals

Cle

an

Co

mp

on

en

ts

Clean

Bottles an

d T

ubes

Clean

Fluid

ics Cover

Clean

Disp

ense H

ead

Clean

Plate Carrier S

ystem

Clean

Prim

ing T

rough

Clean

Com

presso

r

Deco

nta

min

atio

n

Deco

ntam

inate E

xternal S

urfaces

Deco

ntam

inate th

e Fluid

Path

As N

eed

ed

Main

ten

an

ce

Rep

lace solen

doid

valve assemblies

Rep

lace fluid

path

assembly

Op

era

tor’s In

itials

Chapter 6

Troubleshooting

This chapter lists problems that you may experience with the NanoQuot™ Dispenser, and suggests possible solutions for these problems.

Overview................................................................................92 Troubleshooting Charts.............................................................93 Dispenser Start-Up..............................................................93 Fluid Delivery .....................................................................93 Fluid Leakage .....................................................................95 Microplate Carrier Movement ................................................96 Dispense Head Movement ....................................................96 PC-to-Dispenser Communication ...........................................96

92 | Chapter 6: Troubleshooting


Overview

Every effort has been made to ensure that the NanoQuot™ Dispenser is extremely reliable and easy to use. Nevertheless, you could experience problems with the Dispenser hardware, software, and/or accessories. This chapter offers Troubleshooting Charts to help resolve these problems.

The charts list problems, their possible causes, and possible solutions. The charts are categorized as follows:

• Dispenser Start-Up, page 93.

• Fluid Delivery, page 93.

• Fluid Leakage, page 95.

• Microplate Carrier Movement, page 96.

• Dispense Head Movement, page 96.

• PC-to-Dispenser Communication, page 96.

Note: If you are operating the NanoQuot with the Bio-Stack™ Microplate Stacker and encounter problems during plate transfers between the Bio-Stack and the Dispenser, or during communication between the two instruments, refer to the Troubleshooting and Error Codes section of your Bio-Stack Operator’s Manual.

If you cannot resolve the problems, contact BioTek’s Technical Assistance Center (TAC); see Product Support & Service in Chapter 1. You will need to provide TAC with the following configuration information, which is stored in the Dispenser:

• Dispenser software part number (“basecode”)

• Software version To view this information, open the NanoQuot™ PC Control Software. The Dispenser’s basecode part number and version are displayed in the PC Software’s main screen (in the Instrument Software field).

Troubleshooting Charts | 93


Troubleshooting Charts

Dispenser Start-Up

Problem Possible Cause Possible Solution

Power Light (LED) not on.

Power cord not plugged in. Check power connection.

Carrier or dispense head is being obstructed.

Remove obstruction. Clean the plate carrier. See Clean Components in chapter 5.

Motor, sensor, or electrical problem.

Turn Dispenser off, wait at least 15 seconds, then turn back on. If Dispenser does not pass its Self-Test, contact BioTek TAC.

Carrier or dispense head position error.

Misaligned carrier or dispense head.

Contact BioTek TAC.

Fluid Delivery


Inlet tube not connected at distribution manifold or at bottle, or connected to the wrong port on the bottle.

Check all tubing (see Install the NanoQuot Components in Chapter 2).

Supply tube inside the supply bottle is kinked or disconnected.

Straighten or connect supply tube. Make sure the end is properly trimmed.

Solenoid valve clogged. Remove and clean (see Clean the Dispense Head in Chapter 5).

Unable to dispense fluid, or dispense volume is lower than expected.

Inlet tube is touching bottom of bottle.

Make sure inlet is not touching the bottle bottom.

Continued on the next page.



Fluid Delivery, Continued


Incorrect Liquid Profile selected. Select correct Liquid Profile.

Solenoid valves are stuck closed.

Replace valves then run Purge/Prime programs.

No fluid. Fill bottles with appropriate fluid.

System not purged. Run a Purge program.

Supply pressure too low. Raise Inlet pressure to 30 psi, raise Reservoir Pressure.

Unable to dispense fluid, continued

Gas concentration different than Calibrated. Use same initial conditions.

Incorrect Plate Type selected. Select correct Plate Type.

Volume too large for the vessel.

Program a smaller Volume.

Pressure too high. Lower Reservoir Pressure.

Plate overfills (floods).

Fittings to distribution manifold are leaking.

Reconnect/reseat the fittings.

Loose fittings. Tighten or replace loose fittings.

Tubing not adequately primed.

Run a Purge program.

Fiber on valve tip. Remove fiber from the tip.

Uneven dispensing of fluid; wells not filled. Setscrew not tightened on

the solenoid valves; loose valves.

Seat valves and tighten.

Dripping dispense tips. Solenoid valve clogged.

Replace valve then run Purge/Prime programs.

Erratic dispense volumes with one channel.

Kink in dispense tubing from distribution manifold to solenoid valve.

Replace tubing.

Imprecise dispensing to 1536-well plates.

A1 location incorrect. Use Define A1 Locations in the NanoQuot™ PC Control Software to adjust the A1 location.

Troubleshooting Charts | 95


Fluid Delivery, Continued


Improper Purge/Prime after valve replacement.

Run Purge/Prime programs again.

Air in the valve.

Run a Purge program using 70% isopropyl alcohol instead of reagent or water; raise reservoir pressure to 15 psi.

Valve crushed by setscrew. Replace valve then run Purge/Prime programs.

Valve bias setting too high. Use Valve Bias Settings in NanoQuot™ PC Control Software to adjust, if necessary.

Solenoid valve dispensing too high.

Incorrect valve.

Remove valve and check rating (should be 12v). If incorrect, replace valve, then run Purge/Prime programs.

Valve pinched (casing bent).

Replace valve then run Purge/Prime programs.

Supply pressure too low (fluid balls on the end of the valves; fluid too thick).

Raise inlet pressure to 30 psi max, raise reservoir pressure.

Clog in valve seat. Run Purge/Prime programs.

Solenoid valve dispensing too low.

Valve bias setting too low. Use Valve Bias Settings in NanoQuot PC Control Software to adjust, if necessary.

Use the Tip Selections feature in the NanoQuot PC Control Software to select and then test problem valves.

Fluid Leakage


Defective connector. Replace fitting. Fluid leaking from external tubing connector. Worn tubing. Replace tubing.



Microplate Carrier Movement


Microplate not properly seated or strips not level.

Reseat the microplate in the carrier, or the strips in the holder. Make sure the carrier is clean (see Clean the Plate Carrier System in Chapter 5).

Dispense head not compatible with plate.

Only 96-, 384-, and 1536-well plates are supported.

Dispense tip(s) bent.

Gently attempt to straighten the tip using your fingers. If it remains bent, contact BioTek TAC.

Dispense tips not dispensing into wells correctly.

Dispense head tilted. Tighten dispense head screws (PN 12174).

Carrier position error.

Carrier movement is blocked.

Check for any obstruction of the carrier.

Dispense Head Movement


Check orientation of microplate; A1 should be in the left rear corner of the plate carrier as you face the front of the instrument.

Check for and remove any obstructions.

Dispense head position error.

Movement is blocked.

Contact BioTek TAC.

PC-to-Dispenser Communication

Problem Possible Solutions

Error when attempting to communicate with the NanoQuot from the PC Control Software

Select a different COM port. Check for loose cable connections. Ensure that ‘Manual’ mode is selected on the dispenser.

Appendix A

Error Codes

This appendix lists Error Codes that may be retained in the Dispenser and displayed via the PC interface. If an error is displayed, refer to Chapter 6, Troubleshooting.

Overview................................................................................98 Errors During Operation with the Bio-Stack .................................99 NanoQuot™ Instrument Errors...................................................100 PC Software Errors...................................................................102

98 | Appendix A: Error Codes


Overview

Errors that may occur during operation of the NanoQuot™ include instrument errors generated by the Dispenser as well as PC software errors found by any of the PC components running with the NanoQuot.

In order to retrieve error codes retained in the Dispenser, you will need to connect a host computer and install the NanoQuot™ PC Control Software on the computer.

Refer to Chapter 2, Installation, for instructions for connecting a host computer to the NanoQuot and for installing the NanoQuot PC Control Software.

To retrieve error codes in the NanoQuot PC Control Software:

1. Close any Liquid Profile or Dispense Program windows to return to the PC Software’s main screen, if necessary.

2. Click the Get System Status button to display any current error codes.

3. Click OK to clear the error, or refer to the tables in this chapter to identify the cause and remedy for the error.

4. After you clear the error, click Home All Axes.

5. If desired, open the Log File to display the error conditions history: At the PC Software’s main screen, select Tools > View Log File. The ‘Log File Summary’ dialog will appear.

• To save and print the Log File as a report: At the Log File Summary dialog, click Tools > Create Log Report. Launch Windows Notepad. Select File > Open. Browse to the report storage location and select File > Print.

• To purge the Log File: At the Log File Summary dialog, click Tools > Clear Log File. This will permanently delete its records.

Instrument errors will be displayed as hexadecimal codes (e.g., 0x0200); PC software errors will appear in a negative value format (e.g., -2, -100).

See page Error! Bookmark not defined. for a list of NanoQuot instrument errors. See page 102 for a list of PC software errors.

Errors During Operation with the Bio-Stack | 99


Errors During Operation with the Bio-Stack

To view Bio-Stack™ error codes during operation of the NanoQuot™ with the Bio-Stack Microplate Stacker, you will need to disconnect the 9-pin to 9-pin male/male serial cable that connects the Bio-Stack to the NanoQuot, and connect the 9-pin to 9-pin female/male serial cable from the host computer to the Dispenser.

Errors that are displayed (via Get System Status in the NanoQuot™ PC Control Software) may be NanoQuot instrument errors or Bio-Stack instrument errors; the software will provide this information.

Refer to the Bio-Stack Operator’s Manual for more information on errors during operation of the NanoQuot with the Bio-Stack.



NanoQuot™ Instrument Errors

Instrument errors are generated by the NanoQuot™ Dispenser. For the codes that represent motor errors, the fourth digit of the code represents the affected motor:

0 = carrier motor (x-axis)

1 = manifold motor (y-axis)

For example, error code 0x0200 means that the microplate carrier motor (x-axis) could not find home.

If an error is displayed (via the NanoQuot™ PC Control Software), refer to Chapter 6, Troubleshooting. Contact BioTek’s Technical Assistance Center if further instructions are necessary (see Product Support & Service in Chapter 1).

Internal Error Description Error Code

Brief Description

HOME_NOT_FOUND_X_AXIS 0x0200 Home sensor not found for the x-axis, either during the power-up or during the Home All Axes sequence.

HOME_NOT_FOUND_Y_AXIS 0x0201 Home sensor not found for the y-axis, either during the power-up or during the Home All Axes sequence.

LOST_STEPS_X_AXIS 0x0400 Steps lost for the x-axis during processing. Home the instrument and re-run the procedure. If it continues to occur, it could be a hardware problem.

LOST_STEPS_Y_AXIS 0x0401 Steps lost for the y-axis during processing. Home the instrument and re-run the procedure. If it continues to occur, it could be a hardware problem.

INVALID_REQUEST_TO_BIOSTACK 0x1100 The Bio-Stack™ did not recognize the request from the NanoQuot. Try re-booting the Bio-Stack.

INVALID_PARAMETER 0x1200 Invalid parameter sent down to the NanoQuot as part of the request message.

NanoQuot™ Instrument Errors | 101



Brief Description

NANOQUOT_SERIAL_TIMEOUT 0x1300 Serial timeout on the NanoQuot™, awaiting response from the Bio-Stack™. Verify that the Bio-Stack is powered up and operational, and that the serial cable is connected. We recommend turning on the Bio-Stack first and then the NanoQuot.

OUT_OF_RANGE 0x2800 Some value was out of range.

PROFILE_NUMBER_OUT_OF_RANGE 0x2801 The liquid profile number has to be 1, 2, or 3.

INVALID_PASSWORD 0x8300 Invalid password.

NO_MATCHING_ITEM 0x8301 There is no NanoQuot configuration item with that number.

BAD_CHECKSUM 0x8302 Bad checksum.

NOT_IN_DOWNLOAD_MODE 0x8303 The NanoQuot is not in PC control mode.

NAK_RECEIVED 0x8304 NAK received. The NanoQuot did not receive the request from the PC properly.

INVALID_RESPONSE 0x8305 Invalid response received from the NanoQuot.

TIMEOUT 0x8306 Serial message timeout.

MSG_OBJECT_NOT_SUPPORTED 0x8999 The serial message object sent down to the instrument is not supported by the firmware.



PC Software Errors

During computer control of the NanoQuot™, software errors may be generated by any of the PC components running with the Dispenser.

If an error is displayed (via the NanoQuot™ PC Control Software), refer to Chapter 6, Troubleshooting. Contact BioTek’s Technical Assistance Center if further instructions are necessary (see Product Support & Service in Chapter 1).

Table 1

PC Software Errors


Brief Description

UNKNOWN_STATUS -2 Unknown status returned.

USER_CANCELLED_OPERATION -3 User cancelled the operation.

UNABLE_TO_START_TIMER -4 Unable to start a timer.

INVALID_REQUEST -5 The request is invalid.

UNABLE_TO_PROCESS_REQUEST -6 Unable to process the request.

NO_DATA_RECEIVED_YET -7 No data received yet.

KEEP_LOOKING_FOR_SERIAL_DATA -8 Keep looking for serial data, since nothing has been returned yet.

INVALID_MESSAGE_CLASS -9 Invalid message class.

INVALID_MESSAGE_TYPE -10 Invalid message type.

-100 to –199 File Processing errors

FILE_DOES_NOT_EXIST -100 File does not exist. Check the filename.

UNABLE_TO_OPEN_FILE -101 Unable to open file.

FILE_ALREADY_EXISTS -102 File already exists.

FILE_READ_ONLY -103 File is read only.

INVALID_PATHNAME -104 Invalid pathname.

FILE_WRITE_ERROR -105 File write error.

FILE_READ_ERROR -106 File read error.

-200 to –299 Record Parsing errors

INVALID_RECORD_FORMAT -200 Invalid record format.

INVALID_PARAMETER_LIST -201 Invalid parameter list.

UNKNOWN_RECORD_TYPE -202 Invalid unknown record type.

PC Software Errors | 103



Brief Description

-300 to –399 Field Parsing errors

INVALID_FIELD_OPTION -300 Invalid field option.

FIELD_BELOW_MIN_RANGE -301 Field below minimum range.

FIELD_ABOVE_MAX_RANGE -302 Field above maximum range.

FIELD_TOO_LONG -303 Field too long.

FIELD_TOO_SHORT -304 Field too short.

-400 to –499 Object-Specific errors

OBJECT_CREATION_ERROR -400 Object creation error.

OBJECT_DOES_NOT_EXIST -401 Object does not exist.

INSUFFICIENT_FIELDS -402 Insufficient fields.

NAME_REQUIRED -403 Name required.

-500 to –599 Serial Communication errors

BAUD_RATE_INVALID -500 Invalid baud rate.

DATA_BITS_INVALID -501 Invalid data bits selection.

STOP_BITS_INVALID -502 Invalid stops bits selection.

PARITY_INVALID -503 Invalid parity selection.

SERIAL_PORT_ERROR -504 Serial port error.

SERIAL_WRITE_ERROR -505 Serial write error – failure to write out correct number of bytes.

SERIAL_READ_ERROR -506 Serial read error – failure to read in correct number of bytes.

CHECKSUM_ERROR -507 Checksum error.

SERIAL_NAK_ERROR -508 Serial NAK error. The NanoQuot did not receive the request from the PC properly.

INVALID_DATA_COUNT_RECEIVED -509 Excess data or not enough data received.

INVALID_MSG_OBJECT -510 Invalid serial message object.

INVALID_MSG_BODY_SIZE -511 Invalid message body size.

SERIAL_MSG_TIMEOUT -512 Serial message timeout.

PORT_HANDLE_ERROR -513 Port handle error. Cannot access the COM port.

READ_TIMEOUT_INVALID -514 Read timeout value is invalid.




Brief Description

UNAUTHORIZED_TO_OPEN_PORT -515 Unauthorized to open the COM port.

OUT_OF_RANGE_PARM_FOR_OPEN_PORT -516 Out of range parameter for the open port function.

UNABLE_TO_OPEN_PORT -517 Unable to open the COM port.

UNABLE_TO_CLEAR_TRANSMIT_BUFFER -518 Unable to clear the transmission buffer.

Appendix B

Chemical Compatibility

This appendix lists the name and material(s) of each NanoQuot™ component that will come in contact with dispensed chemicals. If you have questions about compatibility of chemicals with the Dispenser, contact BioTek.

Components and Materials Composition ......................................106

106 | Appendix B: Chemical Compatibility


Components and Materials Composition

Continuous contact with harsh chemicals is not recommended. It is best if the dispenser is rinsed with clean, filtered water after contact with any strong acid, base, or solvent, or after each use, and as part of a daily maintenance routine.

Refer to Chapter 5, Preventive Maintenance for instructions for daily maintenance of the dispenser.

Components Material Composition

Bottle Borosilicate glass

Fittings in bottles, inline fittings Polypropylene, PEEK™ (polyetheretherketone)

Bottle cap Polypropylene

Dispense tips 316 SS (stainless steel)

Solenoid valves EPDM (ethylene propylene diene monomer rubber), PEEK, PPS (polyphenylene sulfide), Epoxy

Distribution manifold PEEK

Carrier Aluminum

Assay plates Polystyrene

Supply tubing FEP (tetrafluorethylene-perfluorpropylene)

Priming/drain trough Polypropylene

Drain tubing Tygon

nanoquot operator's manual · 2014-04-14 · contacting biotek instruments, inc. | vii...

Documents