1. Bacteria based micro robot used in cancer treatment

2. Korean research team has successfully developed First Cancer-treating Nanorobot 3. Introduction Cancer is the disease caused by an uncontrolled division of abnormal cells in a part of the body Chemotherapy , is the conventional treatment but.. Damage growing-healthy normal cells blood and hair cells Not specific and No continuous drug delivery for tissues Negative side effects ( anemia , nausea ) 4. Nano robots in medicine This research offer an alternative way instead of chemotherapy Bacteria based micro robot ( Bacteriobot ) Bacteriobot = genetically engineered Bacteria + micro structure bead ( drug ) 5. Why Nano robots ? Specific .. target cancer cells but not normal cells Fast .. one day make sense with cancer treatment Depends on biodegradable and biocompatible materials optimal drug quantities delivered to the target cells 6. How bacteriobot works? To be effective , bacteriobot moves toward tumors acting as combination of Micro sensor Micro actuator Therapeutic agent 7. Designing the experiment Tumor model : colorectal cancer , breast cancer 4T1 Animal model : CT_26 mouse Bacteria model : ppGpp S. typhimurium strain SHJ2037 8. Making the bacteriobot 9. How to combine Salmonella to capsule ? To deliver drug to tumor cells Salmonella work as mechanical engine Micro capsule work as drug container 10. Engineering the bacteriobot It is a fusion between RT* and BT* presents a novel anti tumor strategy Stage 1 : Interaction between biotin streptavidin Biotin found in all living organisms Attenuated salmonella genetically modified to express biotin on the outer membrane of the cell * RT : Robot technology * BT : Biotechnology 11. High affinity interaction between biotin streptavidin Biotin can conjugate to many proteins easily let that protein to interact to with streptavidin conjugated molecules 12. Stage 2 : Development of flow-free chemotactic microfluidic chamber to evaluate targeting , motility , velocity of bacteriobot This device composed of 3 chambers : Normal cells - Bacteriobots - tumor cells Uses fluorescent dyes to see Nano robots target cells 13. flow-free chemotactic microfluidic chamber 14. Stage 3 : Tumor targeting and localization of bacteriobot It is the chemotactic response of bacteriobot to the [con] gradients of various of cancer cells : NIH/3T3 , CT_26 ( colorectal cancer ) 4T1 ( breast cancer ) Targeting measured by migration of bacteria out of central region toward the side chambers 15. Results 16. References "New Paradigm for Tumor Theranostic Methodology Using Bacteria- based Microrobot."Nature.com. Nature Publishing Group, n.d. Web. 18 Feb. 2014. Done by : Belal Abu Haneya Hashemite University Jordan Supervisor : Dr. Abd Allatif Al Ghzawi