nant xl system - brochure - v1 · .#(($.)(*)/#$;(6:
TRANSCRIPT
The Evolution of Cell Culturingfor Mid- up to Large-Scale Batches
NANT XL System
NANT XL Cartridge
Cell Culture Flask 1175 cm2
Cell Culture Flask 23180 cm2
Aseptic Connectorsand Disconnector
HarvestingBag1 L
Waste Bag4 L
Buffer Bag2 L
AuxiliaryBag 1
100 mL
AuxiliaryBag 21 L
Media Bag3 L
A success story: components you already know, engineered in an easy-to-load, disposable, closed system for safe and cGMP-compliant cell processing.
NANT XL Bioreactor
IntegratedImagingSystem
CO2percentagecontrol
RemovableTray
Two SeparatedCell CultureContainers
Advanced2-axis TiltingPrecise
VolumesManagement
Full automation, built-in microscope with confluency estimation, remote continuous monitoring: the key features for a successful and traceable cell production. Sophysticated technology made simple
thanks to the distinctive features of NANT Automated Cell Culturing Systems.
Aseptic Connections, in seconds
As in all NANT Automated Cell Culturing Systems, the different sections of NANT XL Cartridge are designed to be quickly and
easily connected and disconnected - when required - thanks to the
integration of validated aseptic connectors and disconnectors.
Ready for Downstream Processing
NANT XL System output is a cell suspension in a bag, ready for
virtually any kind of required downstream processing operations,
including those that require welding to other reagents-containing
bags too.
Unique Microscopic Imaging and
Cell Confluency EstimationThe built-in auto-focus microscopic imaging automatically
acquires and calculates Cell Confluency. The ideal feature to identify the optimal timeframe for cell harvesting - or just to see
cells growing in real-time.
Smart Process Monitoring
All key process parameters as Temperature, CO2 mixture
percentage and Cell Confluency always displayed on user interface. An intuitive, smart depicting of the current protocol
phase: everything at your fingertips.
Remote Monitoring
Special Web Application allows full, real-time remote monitoring
of in-process control information. No expensive and
time-consuming personnel access to the cell room required.
Subculturing, automatically
Automated subculturing from an initial 175 cm2 flask to a subsequent 3180 cm2, 5-layered container. A competitive feature
allowing to obtain large amounts of expanded cells even from
small quantities of starting material.
175 cm2 3180 cm2
Highlights
• Automated and flexible cell expansion system with an integrated sub-culturing process, for mid- up to large-scale batches.• Remote monitoring of culture conditions and in-process control.
• Consistent results in terms of yield, viability and quality of expanded MSCs.
• Flexibility of the system for different types of cell cultures, both in adhesion and suspension.• Full traceability of operations, disposables and identification of users, with final cell culture report downloadable as PDF.
Amount of starting material
Days of cultureCell Culture
Surface (cm2)
No. of cells harvested
Density of harvested cells
(no. of cells/cm2)
Viability of harvested cells
(%)
1x106
pre-expanded MSCs
8 ± 1175 cm2
to 3180 cm2
5 layers flask2.72 x 108
± 2.55 x 1078.56 x 104
± 8.00 x 10396.0
Representative images of MSCs taken by the NANT XL System during cell culture
0
20
40
60
80
100
CD45 CD14 CD31
% Positive markers expression % Negative markers expression
0
20
40
60
80
100
CD73 CD90 CD166 CD105
Example of Cell Expansion with the NANT XL System
ORDERING DETAILS
ItemNANT XL BioreactorNANT XL Cartridge
ENQUIRIES AND SERVICE
VivaBioCell S.p.A.
Via del Cotonificio, 127 - 33100 Udine, Italy+39 0432 54 56 [email protected]
Catalogue NumberNXB-01NXC-01
Technical Specifications
35 – 55 mL (175cm2)650 - 2000 mL (3180 cm2)Up to 3 LUp to 2 LUp to 100 mLUp to 1 LUp to 1 LUp to 4 L
PolystyreneEVA, EVOH Tygon® and SiliconePharmed® BPTPTFE and PES
>25 kGyUnidirectional sampling valvesGenderless sterile-septumconnectors and disconnector
Working volumes
Cell Culture Flask 1Cell Culture Flask 2
Media Bag
Buffer BagAuxiliary Bag 1Auxiliary Bag 2Harvesting Bag
Waste Bag
Materials
Cell Culture FlaskBags (UV-safe)
Tubing (fluid path)Tubing (pump segment)
Gas Filters (0,22 µm)
Other info
β-rays Irradiation DoseSampling Ports
Sections Connections
71 x 72 x 79 cm28,0 x 28,3 x 31,1 in
64 kg141,1 lbs
15-30°C59-86°F
1-120 mL/min
7” resistive touch-screen
Wireless remote process controland ethernet port
Barcode-based User andCartridge identification
Web application-based access tokey process parameters andmicroscopic images
Automated recording ofuser-dependent operations,key process parameters (temperature,% CO2, cell confluency), warning log,sampling log.
100-240 VAC, 50-60 Hz
Dimensions(W x D x H)
Weight
Operating
Temperature
Pump Flow Rate
User Interface
Connectivity
Traceability
Remote Access
Cell Culture Report
Information
Power Supply
NANT XL Bioreactor NANT XL Cartridge
NANT XL System is designed for Research purposes and any use for human therapy must be conducted under the full responsibility of the user.
VivaBioCell S.p.A. is part of NantCell, Inc., a subsidiary of NantWorks, LLC.www.vivabiocell.it
vivabiocell.it/nantxl
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