Nasonia vitripenniNasonia vitripenniYou are the You are the PIPI ( (PPrimary rimary IInvestigator)nvestigator)

Jewel WaspJewel WaspTell me about it…Tell me about it…

Life CyclesLife Cycles

Haplo/Diplo GeneticsHaplo/Diplo Genetics

Wasp Development Wasp Development TimelinesTimelines

1818oo, 28, 28o o CC

ComplementationComplementation

Process by which an allele of each Process by which an allele of each of two mutant genes makes up for of two mutant genes makes up for

the defect in the other gene.the defect in the other gene.

You Find Three Mutants With a You Find Three Mutants With a Similar Phenotype Similar Phenotype

Are they the Same Genotype?

Possible PhysiologyPossible Physiology

Enzyme A

Enzyme B

Enzyme C

Enzyme A

Enzyme B

Enzyme C

Enzyme A

Enzyme B

Enzyme C

Enzyme A

Enzyme B

Enzyme C

ComplementComplement

a

|

|

B

a

|

|

B

A

|

|

b

A

|

|

b

x

A

|

|

b

a

|

|

B

Two genes

Fail to ComplementFail to Complement

A

|

|

b

A

|

|

b

A

|

|

b

A

|

|

b

x

A

|

|

b

A

|

|

b

1 gene

Three scarlet eyed mutants, how many Three scarlet eyed mutants, how many loci?loci?

ststDRDR,, ststCC,, stst318318

Connect same gene with a line.

stDR

st318

stC

Fail to complem

ent

For example.

HandlinHandling Waspsg Wasps

Sexing WaspsSexing Waspsadultsadults

Sexing WaspsSexing Waspslarvae, tuxedo – black stageslarvae, tuxedo – black stages

Sex PicturesSex Pictures

Scoring Red EyesScoring Red Eyes

Here

Scoring Eye ColorScoring Eye Color

Another Chart, Same Another Chart, Same InfoInfo

Wasp BusinessWasp Business

Link

To DoTo DoWednesdayWednesday

ststDRDR,, ststCC,, stst318318

F1 data, F2 data that means somethingF1 data, F2 data that means something

FridayFriday