national institute of research and development for biological sciences under the authority of...

NATIONAL INSTITUTE OF RESEARCH AND NATIONAL INSTITUTE OF RESEARCH AND DEVELOPMENT FOR BIOLOGICAL SCIENCESDEVELOPMENT FOR BIOLOGICAL SCIENCES

www.dbioro.euwww.dbioro.eu

under the authority of

Ministry of Science and Education of Romania

National Authority for Scientific Research of

RomaniaAddress:

Splaiul Independentei, no. 296, sector 6,

postal code 060031, C.P. 17-16

Bucharest, Romania

Phone: +4021-220.77.80

Fax: +4021-220.7695

E-mail: [email protected]

Brussels, November 2008 National Institute R&D for Biological Sciences


Organizational structureOrganizational structure

The institute was founded in 1996 when the following research institutes

• The Biological Research Institute Cluj (founded 1948),

• The Biological Research Institute Iasi (founded 1956). • The Biological Research Centre “STEJARUL” Piatra Neamt (founded in 1956)

were brought together under the same umbrella.

•The main task of the institute is the fundamental and applicative research in the vast field of life sciences.

•To promote excellent quality research and development, advanced education and technology transfer, ensuring national and international competitiveness in the domains of Biology, Biotechnology, Cellular and Molecular Biology, Microbiology and Ecology.

•To develop and transfer technologies in bioproducts.

The Institute works in close cooperation with universities from all over the country and abroad, with hospitals, other research centres and economical agents working in the field of pharmacology.



Main activities of INCDSB

Frame Programme 7 topics Departments of the Institute

Biology and medicine (health, medical biotechnology);

Agriculture and food supply (food and food

safety issues, fisheries, agricultural

biotechnology);

Environment and climate (environmental

protection);

Information and communication

technology (key words: discrete models,

proteines, health).

-Cellular and Molecular Biology

-Biotechnologies

-Biomatherials and Bioproducts

-Bioanalysis and Bioinformatics

-Microbiology

-Ecotoxicology

-Ecology, Taxonomy, Biological

Control, Resources and

Environment

The domains of bio-, nano- and information technology have an interdisciplinary character.

They contribute to different industrial sectors and policy objectives, they are synergic and sometime complementary.

That is why our research themes are interaction between the topics from FP7 and that is why our research fields are referring to:


Cross-thematic approaches

•Systems biology/bioinformatics – relevant to themes: Health and Information and communication technology

•Factors impacting health – relevant to Health

•Biodiversity and its sustainable exploration for the production of biological resources - relevant to Environment, which deals with the conservation of biodiversity and natural resources

•Novel biotechnology approaches and improved biotechnologies for the production of renewable biomaterials- relevant to biomaterial research under Nanosciences, Nanotechnologies, Materials and new Production technologies


Cross-thematic approaches

Personnel


200 people working in the main office and in the branches.

120 researchers working in the main office and in the branches 65% having a PhD degree 2 scientific coordinators Average researchers age - under 40 yearsThe percentage of PhDs

65% 21%

14%

Doctors

Trainers for a doctor's degree

Research workers

Where do the funds come from ?

National and international competitions

National Programme II PartnershipsCapacitiesIdeas

25 national programmes as coordinators and 100 as partners

International projects: FP6, FP7

Bilateral agreements


EU Projects - Present and Forecast

Theme: 'Food, Agriculture and Fisheries, and Biotechnology'

FP6-STREP Contract FOOD-023044/2006Ready to eat food for breakfast and sport activity with high content of nutraceutics

reducing a disease risk and promoting public healthCoordinator CNR Italy

The project aims the realisation of ready-to-eat food with a high content of compounds endowed with functional activity like carotenoids, alliin and allicin, phenolics, rosmarinic acid, ferulic acids and derivatives (e.g. curcumin). Main objectives of the Nutra-Snacks project:•Selection of organisms producing natural biologically active metabolites with recognized antioxidant activity.•Realization of a bioreactor/sensors for innovative plant cell and in vitro systems suitable for nutrient production•Development of safety and quality control protocols for the new ready-to-eat-food.

Theme: ‘Environment and Climate Change' FP6- SUSTDEV – 2005 - 3.III.1.1

Challenges in assessing and forecasting biodiversity and ecosystem changes in EUROPE

Coordinator Universite Joseph Fourier, France

Advanced modelling approaches has been used so far to assess the impact of global change on biodiversity and ecosystems. These approaches yield projections of the distribution of species, communities and biomass and the functioning of ecosystems. Future goods and services are then asessed from these projections. However four main limitations remain associated to these approaches.



FP7-SME-2008-1 - Research for the benefit of specific groups232522/2008

Biosensors and Sensors for the industrial biosynthesis process of widely used commercial antioxidants: nutraceuticals as additives for food and aquaculture

promoting public health and safetyCoordinator BIOSENSORS Italy

The purpose of this 2-years project is to develop sensors and biosensors for on-line monitoring growth parameters ofindustrial bioprocesses for the production of algal biomass and antioxidant compounds such as Xanthophylls.

EU Projects - Present and Forecast

Finalized International Projects

IntraBioaDiv - Specific Targeted Research Project 2004 – 2006

Title: “Tracking surrogates for intraspecific biodiversity: towards efficient selection strategies for the conservation of natural genetic resources using comparative mapping and modelling approaches”

Coordinator Université Joseph Fourier, Grenoble, France

Realisation of a Chorological Atlas for subalpine and alpine panerogam species of the Carpathian mountains and Alpes within Gloria Europe, INTRABIODIV international projects.

Realisation a synthesis for conservation stade of Romanian Natural Reservation subalpines and alpines panerogam species – international projectsBrussels, November 2008 National Institute R&D for Biological Sciences

Bilateral Agreements

Universities of Montpellier, Perpignan, Lyon, Strasbourg (France), University of Bari (Italy), University of Athens (Greece)

Unité mixte de Rechérche CNRS/USTL No 8576 Franta, Director: Profesor Jean Claude Michalski, Pr. Emerit Jean Montreuil

Université des Science et Technologie de Lille, Laboratoire de Glycobiologie Structurale et Fonctionnelle

Universitaté Paris 7, Laboratoire EMI - U 9922 INSERM – Centre Hospitalier Bichat, France, Profesor Jean Claude Ameisen

Les Laboratoires Pharmaceutiques MacoPharma, Tourcoing, France, Directeur General Francis Goudaliezl

Institut Pasteur Paris, Unite de Physiopathologie des Infections Lentivirales, France, Dr.Jerome Estaquiez


Facilities


Biological Compounds Separation, Identification & QuantificationChromatography: •High-liquid chromatography– diode array detection •Bidimensional Ultra Flow Liquid Chromatography – Fluorescence detection-diode array detection- MALDISpectrometry: Fourier Transformed InfraRed, UV-Vis, FluorescenceElectrochemistry: quartz crystal microbalance, electrochemical systems AUTOLAB, VOLTALABCapillary electrophoresis

Proteomics:

•Matrix Assisted Laser Desorption Ionisation-Time of Flight analysis


Cellular and molecular biology, biomaterial characterisation:•Flow cytometer•Cell separator•Transmission Electron Microscope•Advanced Biosafety Cabinet Class II•Laboratory CO2 incubator•Inverted microscope Zeiss

Facilities

Facilities The Cell Culture Laboratory

set up in order to implement the specific European directives (93/42/EEC)

Aim: to replace in vivo testing on laboratory animals

In vitro cytotoxicity assessing of medical devices; Currently in process of accreditation according to SR EN ISO/CEI 17025:2005 standard directives.


Results 2007 – 2008 Papers

1. Influence of polysaccharides from Viscum album L. on human dermal fibroblasts in vitro, Craciunescu O., Balan M., Gherghina E., Moldovan L., Planta Medica, 73(9), p. 900, 2007

2. Antioxidant activity, phenol and flavonoid contents of some selected Romanian medicinal plants, Alexandru V., Balan M., Gaspar A., Coroiu V., Planta Medica, 73(9), p. 905, 2007

3. ESEM observations on new polyurethane-based materials for biomedical applications: structure and cell adhesion, Craciunescu O., Moldovan L., Bojin D., Vasile C., Zarnescu O., Acta Microscopica, 16 (1-2, Suppl. 2) p.199-200, 2007

4. Lipid nanostructures as carriers for targeting pharmacological active agents; formulation and in vitro studies, Trif M., Moisei M., Chelu F., Moldovan L., Craciunescu O., Roseanu A., Proceedings of European and International Forum on Nanotechnology, Dusseldorf, Germany, 80-82, 2007

5. Nouveaux critères d’évaluation de la viabilité des hématies destinées à la transfusion Mitrofan-Oprea L., Palii C., Tissier J-P., Héron A., Verpoort T., Behague M. , Goudaliez F., Smagghe E., Schooneman, F. Huart J-J., Montreuil J., Bratosin D., Transfus. Clin. Biol., 14, 393-401, 2007

6. Simulating FAS-induced apoptosis by using P systems, S. Cheruku, A. Paun, F.J. Romero-Campero, M.J. Perez-Jimenez, O.H. Ibarra; Progress in Natural Science, 17(4), 424-431, 2007, (ISI Impact factor : 0.531); Review: review number in MathSciNetdata base: MR2332695 (2008e:92015)Brussels, November 2008 National Institute R&D for Biological Sciences

7. Normal forms for spiking neural P systems, O. H. Ibarra, A. Paun, Gh. Paun, A. Rodríguez-Patón, P. Sosík, S. Woodworth; Theoretical Computer Science 372(2-3), 2007, 196-217. (ISI Impact factor: 0.843). Review: review number in MathSciNet data base: MR2303146 (2008a:68058)

8. Small universal spiking neural P systems, A. Paun, Gh. Paun, BioSystems, 90 (1), 2007, pp. 48-60. (ISI impact factor:1.080)

9. The influence of refugial population on Lateglacial and early Holocene vegetational changes in Romania, Feurdean, A., Wohlfarth, B., Bjorkman, L., Tantau, I., Bennike, O., Willis, K.J., Farcas, S., Robertsson, A.M., 2007, Review of Paleobot. & Palynology, 145, 305-320. Impact factor: 0.886

10. Weak and variable relationships between environmental severity and small-scale

co-occurrence in alpine plant communities, Dullinger S., Kleinbauer I., Pauli H., Gottfried M., Brooker R., Nagy L., Theurillat J.-P., Holten J.I., Abdaladze O., Benito J.-L., Borel J.-L., Coldea G., Ghosn D., Kanka R., Merzouki A., Klettner C., Moiseev P., Molau U., Reiter K., Rossi G., Stancisi A., Tomaselli M., Unterlugauer P., Vittoz P., Grabherr G., 2007, J. Ecol. 95, 1284-1295, 2007. Impact factor: 2.7

11. Cryopreservation of carnation (Dianthus caryophyllus L.) shoot tips by

encapsulation-vitrification, Halmagyi, A., Deliu, C., 2007, Scientia Horticulturae, 133(3), 300-306. Impact factor: 0.697



12. The procedure to obtain an electrode with chemical modified surface using L-cisteine and metallic ion determination with consequences in physiological processes Vasilescu, I., Litescu, S., Penu, R., Radu, G.L., 2007, Chemistry Journal, 58 (12), pp. 1101-1166

13. Determination opf hydrosoluble vitamins content and antioxidant capacity of some yeast strains with probiotic potential, Teodor, E., Birsan, C., Smarandache, D., Penu, R., Radu, G.L., 2007, Chemistry Journal, 58 (12), pp. 1249-1252

14. Composite membranes on the basis of new nanomaterials in the class MCM-41 modified with metals and SAPO applied in wastewater purification, Roman, G.P., Parvulescu, V., Radu, G.L., Su, B.-L., 2007, Chemistry Journal, 58 (1), pp. 98-101

15. Stress and displacement in cantilever-based transducers for biosensing application, Bineva, I., Voicu, R., Esinenco, D., Dinescu, A., Muller, R., Bucur, B., Diaconu, M., Radu, L.G. ,2007, Proceedings of the International Semiconductor Conference, CAS, 1, art. no. 4063200, pp. 223-226

16. Improvement of the storage stability for biosensors based on alcohol oxidase immobilized by entrapment in a photopolymerisable matrix, Bucur, B., Bucur, M.P., Radu, G.L., 2007, Chemistry Journal, 58 (11), pp. 1145-1148



17. On the Hopcroft's minimization technique for DFA and DFCA, A. Paun, M. Paun, A. Rodriguez-Paton, Theoretical Computer Science, Accepted, 0.843

18. Discrete nondeterministic modeling of the FAS pathway, J. Jack, A. Paun, A. Rodriguez-Paton, International Journal of Foundations of Computer Science, Accepted

19. Computing with Cells: Membrane Systems - Some Complexity Issues, O.H. Ibarra, A. Paun, Accepted at Journal of Cellular Automata

20. Spiking neural P systems with extended rules: universality and languages, Chen, H.; Ionescu, M.; Ishdorj, TO; Păun, A.; Păun, Gh.; Pérez-Jiménez, M.. Nat. Comput. 7 (2008), no. 2, 147-166. Review: review number in MathSciNet data base: MR2403208

21. On spiking neural P systems and partially blind counter machines Ibarra, Oscar H.; Woodworth, Sara; Yu, Fang; Păun, Andrei. Nat. Comput. 7 (2008), no. 1, 3—19. Review: review number in MathSciNet data base: MR2407929

22. Sequentiality Induced by Spike Number in SNP Systems, Pre-proceedings of Fourteenth meeting on DNA Computing (DNA14), O.H. Ibarra, A. Paun, A. Rodriguez-Paton, June 2-6, 2008, Prague, Czech Republic, pp. 36-47



23. Hopcroft's minimization technique: queues or stacks?, A. Paun, M. Paun, A. Rodriguez-Paton, CIAA 13, July 21-24, 2008, San Francisco, USA

24. Effects of HIV-1 Proteins on the Fas-Mediated Apoptotic Signaling Cascade: A Computational Study of Latent CD4+ T Cell Activation, J. Jack, A. Paun, A. Rodriguez-Paton, accepted at Ninth Workshop on Molecular Computation, WMC9, Edinburgh (UK) July 28-31, 2008, 20pp

25. Determination of S-adenosylmethionine and S-adenosylhomocysteine from human blood samples by HPLC-Fl, Camelia Birsan, SC Litescu, N Cucu, GL Radu, 2008, Analytical Letters, 41(10), 1720-1731

26. EPDM-HDPE blends with differentcure systems/mechanical and infra-red spectrometric properties, Moldovan Z, Ionescu F, Simona Litescu, I Vasilescu, GL Radu,, 2008, Journal of Applied Sciences 8 (1), 86-94

27. Genetic characterisation of some Romanian red wine grapevine varieties, LC Ghetea, RM Motoc, AM Niculescu, SC Litescu, VF Duma, CF Popescu,, 2008, Progress in Biomedical Optics and Ima ging-Proceedings of SPIE 6991, art no 699128

28. Analysis of methanol-ethanol mixtures from falsified beverages using a dual biosensors amperometric system based on alcohol dehydrogenase and alcohol oxidase, Bucur, B., Radu, G.L., Toader, C.N., 2008, European Food Research and Technology, 226 (6), pp. 1335-1342



29. Obtaining and caracterization of biocompatible supports as microparticles and chitosan-alginate films with immobilized urease, Roman, G.P., Neagu, E., Radu, G.L., Teodor, E., 2008, Chemistry Journal, 59 (2), pp. 208-211

30. Optimization of hydroxyl radical formation using TiO2 as photocatalyst by response surface methodology, Eremia, S.A.V., Chevalier-Lucia, D., Radu, G.-L., Marty, J.-L., 2008, Talanta. Article in Press

31. Inhibition of Low - Density Lipoprotein Peroxidation by BHA use. Fluorimetric Assay,Sandra Eremia, SC Litescu, JL Marty, GL Radu, accepted 2008, in press Analytical Letters

32. The procedure to obtain an electrode with chemical modified surface using L-cisteine and metallic ion determination with consequences in physiological processes, Ioana Vasilescu, Simona Liţescu, R. Penu, G. L. Radu 2007, Chemistry Journal, 58(12), 1101-1106



Experimental Techniques in Bioanalysis - ISBN 973-718-373-8 Bioinformatics - Vol I : Molecular computing and simulating methods ISBN (10) 973-31-2299-8

Romanian Biological Sciences (since 1998) - ISSN 1584-0158


Results Books and journals

Other over 10 books

Produced by PlantaVorel:

1. Products with antiiflamatory and antirheumatic actions: REUMA- solution and REUMA- cream that contains the phytocomplex with antiinflamatory properties and of stimulating the circulatory system, produced from Amoracia rusticana

2. Product with a detoxifying action: DETOX A - tablets with a complex action of eliminating the residues and toxic substances from the organism, assuring its rebalancing

3. Products with antioxidant action: ANTIOXIDANT - capsules and OREGANO hydroalcoholic solution, preventing the formation of the free radicals by an adequate contribution of antioxidants with the aim to maintain a metabolic balance essential to the health state of the organismBrussels, November 2008 National Institute R&D for Biological Sciences

Results Products

Products based on the results of the research made in our institute

Produced by PlantaVorel:

4. Products with immunostimulating, adaptogen, general tonic actions: IMUNOSTIM A - tablets and BASIL - glycerinated solution with a complex, general tonic action of the organism functions and of increasing the unspecific defending resistance of the organism

5. Products with sedative, cardiotonic action: CALMOTON - cardiotonic solution with a complex action on the central nervous system, neurovegetative and against the functional vegetative disorders of the heart


Results Products


Produced by Hofigal:

Gamut of Bio-products based on matricial biopolymers and plant extracts used in the treatment of tissue diseases, improving and sustaining the health of the orgamism.

These products are biocompatible, non-allergenic and biodegradable.


Results Products


1. PLANTINTIM – The product is a gel formula based on bioactive principles, essential oils with an marked antiseptic effect.

Produced by Hofigal:

2. Bucoprotect – This gel can be used in the treatment of painful and bleeding gums. It can be also used in inflammatory diseases of the mouth This bio-product has antiinflamatory, antibacterians, antifungic and cicatrizing effects.

3. Epifin - a formula that contains hidrosoluble collagen, an vegetable oil rich in vitamines, and a volatile oil with antiseptic effect.


Results Products


Diplomas and gold medals were achieved at the International Inventica Saloon – Geneva 2002, 2003, 2004 and in the Worlds Showroom Eureka in Brussels 2003 for bioproducts based on vegetal and animal extracts.


Results Patents

Methode pour stimuler la proliferation de cellules differenciees appurtenant au lignage chrondrogenique, French patent No. 0707113/10.10.2007. Authors: Bratosin Daniela, Buia Luminita, Heron A. Montreuil Jean.. INTERNATIONAL PATENT

Afinity support and obtain proceedingd for heparine. PATENT No. 98-01052/04.06.1998, Decision No. 3/404/27.10.2005. Authors: Luminita Tcacenco, Ghiocel Radu Paul, Maria Caloianu, Eugenia-Dumitra Teodor, Lucia Moldovan, Daniela Pomponiu.

Chromatographic method for polyphenols determination, Patent application No. A/00766/30.09.2008. Author: Gatea Florentina.

Chromatographic method for antocyanidins determination, Patent application No. A/00765/30.09.2008. Author: Gatea Florentina.

Characteristics improvement for simultaneous analysis of methanol and ethanol without analytes separation by using a biosensor based on AOX extracted from Hansenula polymorpha”, Patent application No. A696/11.09.2008 Authors: Bucur Bogdan, Bucur Madălina Petruța, Radu Gabriel Lucian, Rădulescu Cristina.

Biopolymeric materials based on mixtures of polyurethane and natural polymers and preparation method, Patent application No. A/00539/2008. Authors: Lungu Maria, Moldovan Lucia, Craciunescu Oana, Zarnescu Otilia, Vasile Cornelia

Biocompatible materials prepared by polyurethane cover with biopolymers and preparation method, Patent application No. A/00468/2008. Authors:Moldovan Lucia, Lungu Maria, Craciunescu Oana.


Results Patents and patent applications

Nanostructured material based on magnetic nanoparticles and hydrogel, and its obtain process, Patent application No. A/00453/26.06.2007. Authors: Eugenia Teodor, C. Petcu, Veronica Lazăr, Mihaela Mihalache.

Obtaining method for lypase with Yarrowia lipolitica stem, Patent application No. A/001 178/08.03.2007. Authors: Luminita Tcacenco, Diana Gabriela Groposila Constantinescu, Camelia Filofteia Diguta, Andreea Cozea, Diana Pelinescu, Elena Sasarman.

Organizing of Scientific Meetings

"Biological Diversity – Biotechnologies – socio-economical development“ - national symposium

"Bioanalysis versus Biotechnology” - Workshop

"Natural Sciences“ – Romanian – Austrian Symposium

"Microbiology and Biotechnology“ - Symposium

National Congress of Biology "Emil Racovita“

"General and applied entomology“ - National Conference

"Entomofaunistics in Central Europe“ - International Symposium

"Medicinal Plants - present and perspectives“ - National Symposium


Thank You !
