national reports - overview of regional status regional workshop 2: improving capacity for diagnosis...
DESCRIPTION
Movement/transfers Between farms within country Importation of eggs/fry/live fish Importation of market sized fish for human consumption Importation of fry for cultivation from neighboring countries and European countries (Hungary, Italy, Bulgaria etc) Importation of eggs mainly from Denmark and USATRANSCRIPT
National reports -overview of regional
status
Regional Workshop 2: Improving Capacity for Diagnosis of Fish and Molluscan Diseases
Banja Luka, Bosnia and Herzegovina, 20-24 October 2013, Hotel Palas
Production data 2012
Most represented – carp and rainbow troutLess – other cyprinid and salmonid speciesand marine fish (Croatia, Montenegro, BiH)MolluscsMusselsOysters
Movement/transfers Between farms within country Importation of eggs/fry/live fishImportation of market sized fish for human
consumptionImportation of fry for cultivation from
neighboring countries and European countries (Hungary, Italy, Bulgaria etc)
Importation of eggs mainly from Denmark and USA
Diseases recorded in the countries
Viral diseases – IPNV in Croatia, Bosnia and Herzegovina, Serbia
VHSV – single outbreak in CroatiaBacterial diseases – very similar in all countries
– furunculosis, yersiniosis, BKD, flavobacteriosis, carp erithrodermatitis, columnaris disease, motile aeromonas septicaemia (MAS)
Marine fish species – vibriosis, tenacibaculosisParasitic diseases – marteiliosis in mussels in
Croatia and Montenegro; ectoparasites
Fungal diseases – saprolegniosis
Other health problems – Newly described diseases
RMS /strawberry disease
Diagnostic capacity in the countries
Aquatic animal diseases laboratory within country
All coutries some kind of NRL with different responsibilities; research and educational institution dealing with aquatic animal health
Some are mostly educational and other research
Level of diagnostic
Level of diagnostic:Level 1: Gross observation, general
necropsy (external, internal)Level II: Bacteriology, Parasitology,
Mycology, HistologyLevel III: Cell lines, Immunological tests,
TEM, Molecular techniquesMOST COUNTRIES – LEVEL II
Analysis of diagnstic capacity
List of diseases and diagnostic methods used List is more or less same VHS, IPN, SVC, KHV – clinical observation,
detection of gross changes, histological observation, isolation of virus on cell culture, identification by ELISA, PCR,
Furunculosis, yersiniosis, BKD, carp erythrodermatitis, columnaris – Isolation of bacteria on bacteriological media, identification of genus by selective media and general properties, identification of the species by API or any other kit for biochemical identification
Surveillance of nationally listed diseases
Diagnostic methods – VHS, IHNV, PVŠ, IPNV, KHV, BKD – according to the level of lab capacity
Sampling – organised by Competent Authority
Results – presence of IPNV, VHSV, PVŠ not diagnosed in last two years, BKD sporadically
Surveillance and monitoring of other pathogens
Targeted pathogens and aquaculture species –
Croatia – bacteria V. anguillarum and Tenacibaculum maritimum in sea bass, Yersinia ruckeri and Flavobacterium psyhrophilum – antimicrobial resistance
Eustrongyloides sp. – possible human health threat – no programme of control until now
RMS, MAS, ECV – regionally present - due to insufficient funding no surveillance or monitoring programmes
Study of abnormal mortality
Notification of abnormal mortalities in Macedonia, available diagnostic methods performed – mortality caused by abiotic factors
Similar cases in fish farms/ open waters are notified in other counties -
Conclusions and perspectives
Decrease of aquaculture production in BiHImproving progress in harmonisation of
legislative in Serbia, MontenegroUnsufficent laboratory capacity in BiH,
Montenegro and Macedonia (they are not complying with OIE/EU standards for diagnosis of listed viral diseases)
There are no diagnostic methods for crustacean diseases developed in any country in the region
Future needs
Education for insufficient laboratory methodsImprovement of lab equipment and
proceduresEvaluation of diagnostic possibilities through
interlaboratory proficiency testing (maybe it is possible to organise it regionally within this project)
Implementation of diagnostic methods for other species (crustacean)
Regional networking