national seminar on biodiversity · 2019. 2. 21. · time schedule national seminar on biodiversity...

NationalSeminar onBiodiversity

Abs Masy Biodiv Indonvol. 4 | no. 1 |pp. 1-18 | January 2017

ISSN: 2407-8069

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SECRETARIAT ADDRESSSekretariat Masyarakat Biodiversitas Indonesia, Kantor Jurnal Biodiversitas, Jurusan Biologi Gd. A, Lt. 1, FMIPA UNS,Jl. Ir. Sutami 36A Surakarta 57126, Jawa Tengah, Indonesia. Tel. +62-897-6655-281. Email: [email protected]: biodiversitas.mipa.uns.ac.id/snmbi.html

Organized by Selected manuscriptswill be available at

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TIME SCHEDULENational Seminar on Biodiversity

Society for Indonesian Biodiversity (SIB)Depok, Indonesia, 28-29 January 2017

TIME ACTIVITIES PERSON IN CHARGE SITE

January 28, 2017

07.30-08.00 Registration Committee R108.00-08.30 Opening ceremony Chairman of the SIB R1

08.30-10.00 Panel I Moderator R1Prof. Dr. Nurheni WijayantoProf. Dr. Enny Sudarmonowati

10.00-10.15 Photo session and coffee break Committee R1, Lobby

10.15-12.00 Panel II Moderator R1Dr. Mohammed Ali BerawiDr. Tribidasari A. Ivandini

12.00-13.30 Rest, prayer, lunch & Poster session Committee Lobby

13.30-14.30 Parallel presentation IGroup 1: AO-01 to AO-05 Moderator R1Group 2: AO-06 to AO-10 Moderator R2Group 3: BO-01 to BO-05 Moderator R3

14.30-15.30 Parallel presentation IIGroup 4: BO-06 to BO-09 Moderator R1Group 5: CO-01 to CO-04 Moderator R2Group 6: DO-01 to EO-03 Moderator R3

15.30-15.45 Coffee break, prayer Committee Lobby

15.45-16.00 Announcement of the Best Presenters Chairman of the Board ofAssessors

R1

16.00-17.00 The writing style of the journal Biodiversitas andNusantara Bioscience

Editor-in-Chief/Managing Editor

R1

17.00-18.00 Individual coaching *)Group 1, 4 Guest editor R1Group 2, 5 Guest editor R2Group 3, 6 Guest editor R3

18.00-19.30 Rest, prayer, dinner Committee Lobby

iv

19.30-21.30 Individual coaching (cont’d)Group 1, 4 Guest editor R1Group 2, 5 Guest editor R2Group 3, 6 Guest editor R3

21.30 Closing speech and other explanations Chairman of the committee R1

January 29, 201707.30- ... City tour Committee Lobby

Note: *) tentative

Upcoming events:1. February 18-19, 2017 – Malang, East Java (National Seminar and Scientific Research Paper Writing)

http://biodiversitas.mipa.uns.ac.id/S/2017/malang/home.html

2. March 18-19, 2017 – Yogyakarta (International Conference on Biodiversity)http://biodiversitas.mipa.uns.ac.id/S/2017/jogja/home.html

3. April 13-15, 2017 – Palembang, South Sumatra (National Seminar on Biodiversity)http://biodiversitas.mipa.uns.ac.id/S/2017/palembang/home.html

4. May 20-21, 2017 – Jatinangor-Sumedang, West Java (International Conference on Biodiversity)http://biodiversitas.mipa.uns.ac.id/S/2017/bandung/home.html

5. July 5-8, 2017 – Derawan, Berau, East Kalimantan (International Conference on Biodiversity)http://biodiversitas.mipa.uns.ac.id/S/2017/berau/home.html

6. August 12-13, 2017 – Palu, Central Sulawesi (International Conference on Biodiversity)http://biodiversitas.mipa.uns.ac.id/S/2017/palu/home.html

7. September 9-10, 2017 – Bogor, West Java (National Seminar & Scientific Research Paper Writing)http://biodiversitas.mipa.uns.ac.id/S/2017/bogor/home.html

8. October 14-15, 2017 – Pontianak, West Kalimantan (International Conference on Biodiversity)http://biodiversitas.mipa.uns.ac.id/S/2017/pontianak/home.html

9. November 4-5, 2017 – Medan, North Sumatra (International Conference on Biodiversity)http://biodiversitas.mipa.uns.ac.id/S/2017/medan/home.html

10. December 8-10, 2017 – Bali (International Conference on Biodiversity)http://biodiversitas.mipa.uns.ac.id/S/2017/bali/home.html

TABLE OF CONTENTSNational Seminar on Biodiversity


CODE TITLE AUTHOR(S) PAGES

Genetic diversityAO-01 Diversity study of gaga chicken (Gallus gallus

domesticus) based on DNA barcoding analysisPipih Suningsih Effendi,Abinawanto

1

AO-02 Karakteristik genetik ikan lais Ompok spp danKryptopterus spp (Siluridae) asal Sungai MahakamKalimantan Timur berdasarkan gen COI DNAmitokondria

Jusmaldi, Dedy Duryadi Solihin,Ridwan Affandi, Mf Rahardjo,Rudhy Gustiano

1

AO-03 Weak delineation of Styrax species growing in NorthSumatra, Indonesia by matK+rbcL gene

Henti H. Rahmat, AridaSusilowati, Supriyanto, CutRizlani Kholibrina, RiniRamadhani

2

AO-04 Identitas dan keragaman genetik abalon (Haliotisasinina) di perairan wilayah barat dan timur Indonesiaberdasarkan gen COI DNA mitokondria

Ajeng Daniarsih ,, Dedy DuryadiSolihin, Rudhy Gustiano

2

AO-05 Masukan jumlah energi satuan panas terhadap kualitashasil panen babycorn (Zea mays) di dataran rendah

Indra Dwipa 2

AO-06 New polymorphisms of exon 8 of the Growth HormoneReceptor Gene in Pesisir Cattle

Yurnalis, Arnim, Sarbaini Anwar,Dino Eka Putra

3

AO-07 Polimorfisme gen reseptor hormon pertumbuhan (GHR)pada sapi Pasundan, Jawa Barat

Widya Pintaka Bayu Putra, PaskahPartogi Agung, Saiful Anwar,Syahruddin Said

3

AO-08 Keragaman gen growth hormone (GH) pada beberaparumpun sapi lokal Indonesia

Paskah Partogi Agung, SaifulAnwar, Widya Pintaka BayuPutra, Dan Syahruddin Said

3

AO-09 Moleceular phylogeny of Eastern Sumatran Species ofMangifera (Anacardiaceae) within the genus Inferredfrom ITS Sequence

Ibna Hayati, Fitmawati, NerySofiyanti

4

AO-10 Anti-Mycobacterium tuberculosis Strain H37Rv activityagainst Brazilin Compound In Vitro

Ratu Safitri, Aya Sofa Novia W,Mas Rizky A.A. Syamsunarno,,Ani Melani Maskoen

4

vi

Diversity of speciesBO-01 Application of Open Source Software ImageJ for

morphometrical measurements of of bats spermatozoaGenus Cynopterus

Taufiq Purna Nugraha, ImanSupriatna, Srihadi Agungpriyono,Gono Semiadi

4

BO-02 Keanekaragaman jenis serta distribusi populasimammalia arboreal di kawasan Hutan Kemuning, Bejen,Kabupaten Temanggung, Jawa Tengah

Agnes Audina Krisanti, TetriWidiyani, M. Ali Imron

5

BO-03 Diversity of Rhizosphere entomopathogenic fungi ofgroundnut and its virulence against the pod borer, EtiellaZinckenella, in West Sumatra

Reflinaldon, Hasmiandy Hamid,Trizelia, Faridwan Amir, NelpiGusnita, Nova Rosa

5

BO-04 In vitro characterization of indigenous Bacillus spp. tocontrol Bacterial Wilt Disease (Ralstonia solanacearum)and increase growth rate

Yulmira Yanti, Trimurti Habazar,Reflinaldon, Chainur RahmanNasution, Srimano Felia

6

BO-05 Keanekaragaman isolat rizobakteria indigenus danpotensinya sebagai induser ketahanan bawang daunterhadap hama Spodoptera exigua (Lepidoptera:Noctuidae)

Trimurti Habazar, Arneti, IlmaJuita

6

BO-06 Variasi musiman keragaman dan distribusifungi mikoriza arbuskula di areal pertanaman sawit

Delvian 6

BO-07 Conservation of biodiversity aquatic plants from Borneo,Indonesia through tissue culture techniques for freepathogen aquarium scape

Media Fitri Isma Nugraha,, RosaYunita, Endang Gati Lestari,Anjang Bangun Prasetio

7

BO-08 The preferences of lobster fishermen to catching thePainted Spiny Lobster (Panulirus versicolor Lattreille,1804) in South Coast of Java, Indonesia

Hanum Isfaeni 7

BO-09 Keanekaragaman tumbuhan tropis berpotensi sebagaiatraktan alami untuk pengendalian rayap dengan sistemumpan

Yuliati Indrayani,, Musrizal Muin,Tsuyoshi Yoshimura

7

BP-01 Spatial distribution of abundant tree species at a mixeddipterocarps forest in Bukit Bangkirai, East Kalimantanthree years after long drought and forest fire

Mustaid Siregar 8

BP-02 Variability in abundance and diversity of copepods inMond River estuary, Bushehr, Persian Gulf

Aliakbar Hedayati, MojtabaPouladi, Amir Vazirizadeh, AmirQadermarzi, Ahmadreza Jabaleh

8

BP-03 Production and characterization of a neutral phytase ofPenicillium oxalicum strain EUFR-3 from Himalayanregion

Ramandeep Kaur, AbhishakeSaxena, Punesh Sangwan, AjarNath Yadav, Vinod Kumar,

Harcharan Singh Dhaliwal

8

Diversity of ecosystemCO-01 The estimation potential livestock breeding of buffalo in

Ulakan Tapakis Sub-district, Padang Pariaman District,West Sumatra, Indonesia

Dino Eka Putra, Sarbaini, TindaAfriani

9

CO-02 Economic valuation of whale shark tourism inCendrawasih Bay National Park, Papua, Indonesia

Zuzy Anna, Dicky Surya Saputra 9

CO-03 Influence of planting line width on the increment ofShorea leprosula and Dryobalanops lanceolata atSelective Logging Line Planting System

M. Taufan Tirkaamiana, AfifRuchaemi, M. Sumaryono

10

CO-04 Dekomposisi serasah Avicennia alba pada tingkat Kansih Sri Hartini, Yunasfi, Media 10

vii

salinitas yang berbeda di hutan mangrove KampungNipah, Sei Nagalawan, Sumatera Utara

Oktari

CP-01 The population and habitat characteristics of Sundapangolin (Manis javanica, Desmarest 1822) in theBojonglarang Jayanti Natural Reserve, West Java,Indonesia

Ruhyat Partasasmita,,, IrinaAnidya Mustikasari,, Erri NoviarMegantara,

10

CP-02 Kandungan nutrien rumput laut asal Pantai Binuangeun,Propinsi Banten

Lusi Herawati Suryaningrum,Reza Samsudin

11

CP-03 True mangrove species on the remote small islands ofTambelan, Riau Archipelago Province, Indonesia

Ahmad Dwi Setyawan, Yaya IhyaUlumuddin

11

CP-04 Biodiversity of endophytic fungifrom macroalgae(Sargassum sp., Gracilaria sp., Gelidium sp., andCaulerpa sp.) at Pari Island, Seribu Islands, Jakarta

Mujizat Kawaroe,, AdrianiSunudin, Junkwan Hwangbo, AmiShaumi

11

CP-05 Biodiversity of aquatic fauna in mangrove area ofPurworejo District, Central Java

Slamet Mardiyanto Rahayu,

Wiryanto, Sunarto12

CP-06 Important weeds and their control in soybean productionunder rice-soybean cropping patern

Arief Harsono, Suryantini, SitiMuzaiyanah

12

CP-07 Effects of felling intensity on Hymenoptera biodiversityin a natural production forest in East Kalimantan,Indonesia

Ahmad Budiaman, Noor FarikhahHaneda, Ima Duwi Robaikah

12

CP-08 The profile of Dipterocarpaceae in MomiwarenProtected Forest in South Manokwari District, WestPapua, Indonesia

Rima H. S. Siburian 13

CP-09 Effect of Italian Cypress (Cupressus sempervirens var.horizontalis) reforestation on represence native treespecies in Hyrcanian forest (North of Iran)

Leila Vatani, Seyed MosehHosseini, Moslem Akbarinia,Saeed Shamsi

13

CP-10 The pardox of exotic versus indigenous chickenpopulation dynamics and distribution pattern inEthiopia: Review

Kefyalew Alemayehu, TilahunSisay

13

Ethnobiology & SocioeconomicsDO-01 Inventory and comparison of species of plants in Obat

Pahit Herb Ancestors Legacy Of Ethnic Malay ofLingga, Riau Islands

Fitmawati, Nery Sofiyanti,Rodesia Mustika Roza, HidayatulHazimi, Yulisa Resti Irawan,Dhaniel Ridho Winata, AwalPrichatin Kusumo Dewi

14

DP-01 Factors influencing the adoption of rice paddy threshingmachine “Powered-Thresher”: A case study of SerangDistrict, Banten Province

Tian Mulyaqin,, Ishii Keiichi 14

DP-02 The partial budgeting analysis of power thresherutilization for rice farming

Tian Mulyaqin,, Ishii Keiichi 14

DP-03 Society empowerment model, harvesting technique andproduction potential of Trigona spp. in Kahu, SubDistrict of Bontocani, District of Bone District, SouthSulawesi

Budiaman, Octaviani W,Sadapotto A, Iswara Gautama,Andi Mujetahid, Baharuddin,Muhammad Agung

15

BioscienceEO-01 On skeletochronology of Asian grass frog Fejervarya

limnocharis (Gravenhorst, 1829) from Java, IndonesiaNi Luh Putu Rischa Phadmacanty,Amir Hamidy

15

viii

EO-02 Mycorrhizal inoculum potent of the peat soils inTumbang Nusa, Central Kalimantan

Hesti Lestari Tata 15

EO-03 Flowering morphology, flower development and insectvisitors to flowers of Nepenthes mirabilis (Lour.) Druce.

Tri Handayani 16

EP-01 Characterization of cellulase enzyme from thermophilicbacteria isolates based on ph and optimum temperature

Enni Ayu Safitri, MaswatiBaharuddin, Sappewali

16

EP-02 Relationship between Self-directed Learning andAcademic Motivation in Nursing Students

Masoomeh Adib, Shokoh Varaei,Shahrzad Ghiyasvandian, ZahraAtrkar Roushan

16

EP-03 Presence of arbuscular mycorrhizal fungal sporesassociated with corn planted with traditional and moremodern farming systems

Lily Ishaq, Anthonius S. J. AduTae, Moresi A. Airthur, Peters O.Bako

17

Note: A. Genetic Diversity, B. Diversity of Species, C. Diversity of Ecosystem, D. Ethnobiology, E. Bioscience (LifeScience and Technology); O. Oral, P. Poster

ABS SOC INDON BIODIVVol. 4, No. 1, January 2017 ISSN: 2407-8069Pages: 1-18 DOI: 10.13057/asnmbi/m040101

ABSTRACTNational Seminar on Biodiversity


Genetic diversity

AO-01Diversity study of gaga chicken (Gallus gallusdomesticus) based on DNA barcoding analysisPipih Suningsih Effendi♥, AbinawantoPostgraduate Program of Biological Sciences, Faculty of Mathematics andNatural Sciences, Universitas Indonesia. Kampus UI Gedung E Lt. 2, Jl.Lingkar Kampus Raya, Depok 16424, West Java, Indonesia. Tel.: +62-21-7270163 Fax.: +62-21-78849010. ♥email: [email protected]

Diversity study of Gaga chicken (Gallus gallus domesticus)based on DNA barcoding analyses has been studied.Research was conducted during August 2015-January2016. Whole Blood chickens were collected from 17chicken of slow type and 5 chicken of dangdut types,respectively, originated from Sidrap. While, seven bloodsamples were collected from Jakarta, consisted of fourdangdut types and three slow types, respectively. Bloodsamples were kept in the tube containing EDTA, and werestored at-20 ºC. Cytochrome Oxydase subunit I gene wasused as a marker of DNA Barcoding to identify thediversity of gaga chicken from Sidrap or Jakarta. TheBLAST result showed that 50% of sample sequencedconsisted of 700-800 base in length. The reconstructionresult of the phylogenetic tree showed that gaga chickensfrom Bullo village (Sidrap) had closed relatives with gagachicken from Jakarta (Kebayoran Lama) with 89.7% ofbootstrap value. The highest boostrap value (96%) showedbetween gaga chicken from Sidenreng and Bullo village.

Bootstrap, Cytochrome Oxidase Subunit-I, DNA barcoding,gaga chicken

AO-02Karakteristik genetik ikan lais Ompok spp danKryptopterus spp (Siluridae) asal Sungai Mahakam

Kalimantan Timur berdasarkan gen COI DNAmitokondriaJusmaldi1,♥, Dedy Duryadi Solihin2, Ridwan Affandi3,Mf Rahardjo3, Rudhy Gustiano4

1 Department of Biology, Faculty of Mathematics and Natural Sciences,Mulawarman University. Jl. Barong Tongkok No. 4, Gunung Kelua,Samarinda Ulu, Samarinda-75123, East Kalimantan, Indonesia. Tel./Fax.:+62-541-749140, 749152, 749153. ♥email: [email protected] Department of Biology, Faculty of Mathematics and Natural Sciences,Institut Pertanian Bogor, Bogor 16680, West Java, Indonesia.3 Department of Aquatic Resources Management, Faculty of Fisheries andMarine Sciences, Institut Pertanian Bogor, Bogor 16680, West Java,Indonesia.4 Institute of Research and Development for Freshwater Aquaculture. Jl.Sempur No.1, Bogor 16154, West Java, Indonesia.

Ikan air tawar famili Siluridae dari genus Ompok danKryptopterus adalah spesies kompleks dan dikenal dengannama ikan lais. Metode konvensional untuk karakterisasiikan banyak berdasarkan pada perbedaan morfologis,namun metode ini terkadang dapat bias pada kasus spesiescryptic. Metode Polymerase Chain Reaction (PCR)menggunakan runutan gen COI DNA mitokondria sebagaiDNA barkoding memberikan hasil yang lebih pasti untukmembedakan spesies. Total 30 runutan gen COI DNAmitokondria ikan lais (O. miostoma, O. hypophthalmus, K.limpok, K. apogon, K. bicirrhis, dan K. micronema) asalSungai Mahakam diamplifikasi menggunakan primer COIFish F1 dan COI Fish R1, dan ditambah 19 runutan daridata GenBank sebagai spesies pembanding. Hasilpenelitian menunjukkan perbedaan karakteristik genetikberdasarkan gen COI parsial DNA mitokondria sepanjang655 pasang basa (pb) antara genus Ompok danKryptopterus sebanyak 9 single nucleotide polymorphism(SNP), antara spesies O. miostoma dan O. hypophthalmussebanyak 22 SNP dan antara spesies K. limpok, K. apogon,K. bicirrhis, K. micronema sebanyak 73 SNP. Rata-ratajarak genetik antar genus Ompok dan Kryptopterus19,01%. ± 1,68%, antarspesies dalam genus Ompok12,77% ± 0,10% dan antarspesies dalam genusKryptopterus 10,99% ± 0,29%. Konstruksi ulang pohonfilogenetik menunjukkan ada 6 spesies berbeda ikan laisasal Sungai Mahakam.

ABS SOC INDON BIODIV 4 (1): 1-18, Depok, 28-29 January 20172

Ikan bersungut, spesies kompleks, Siluridae, SungaiMahakam

AO-03Weak delineation of Styrax species growing inNorth Sumatra, Indonesia by matK+rbcL geneHenti H. Rahmat1, ♥, Arida Susilowati2, Supriyanto3,Cut Rizlani Kholibrina4, Rini Ramadhani4

1 Forest Research and Development Agency, Ministry of Environment andForestry. Jl. Raya Gunung Batu. Bogor. West Java. Indonesia. Telp/Fax.+62251 8384445/+62251 8334314. ♥email:[email protected] Faculty of Forestry, University of Sumatera Utara. Jl. Tri Dharma UjungNo 1, Kampus USU Medan. North Sumatera Indonesia,3 Faculty of Forestry Bogor Agricultural University. Jl. Ulin Kampus IPBDramaga. Bogor West Java. Indonesia4 Aeknauli Forest Research Agency. Jl. Raya Parapat 10.5, Parapat NorthSumatera. Indonesia

DNA barcoding enables precise identification of speciesfrom analysis of unique DNA sequence of a target gene.The present study was undertaken to develop barcodes fordifferent species of Styrax spp growing in North Sumatera-Indonesia. Four species of Styrax, each represented by twoindividuals, collected from two districts in North Sumaterawere evaluated using two regions in the plastid genome(matK and RbcL) in order to discriminate them at specieslevel. Result showed that matK yielded 891 bp afteralignment, however there was no precise identificationuntil species level in which the matK gene onlydifferentiate the four known species into two group, eachgroup consisted of different species. The RbcL geneyielded 588 bp and showed no variation for all species,those it determined all species into one same haplotype.Furthermore, combined mat K + RbcL will give similarresult to those of matK grouping. Considering the overallperformance of these loci, we suggest matK + RbcL is notstrong enough to determine Styrax growing in NorthSumatera until the species level. These findings showed thenecessity to find other candidate genes or markers that canpotentially be helpful in delineating the various species ofStyrax growing in North Sumatera, as well as other relatedStyrax genera.

Barcoding, matK + RbcL gene, kemenyan, deliniating,haplotype

AO-04Identitas dan keragaman genetik abalon (Haliotisasinina) di perairan wilayah barat dan timurIndonesia berdasarkan gen COI DNA mitokondriaAjeng Daniarsih 1,♥, Dedy Duryadi Solihin2, RudhyGustiano3

1 Program Studi Biosains Hewan, Departemen Biologi, FakultasMatematika dan Ilmu Pengetahuan Alam, Institut Pertanian Bogor. Jl.Raya Darmaga Bogor 16680, West Java, Indonesia. Tel./Fax. +62-251-8622833, ♥email: [email protected]

(2) Departemen Biologi, Fakultas Matematika dan Ilmu PengetahuanAlam, Institut Pertanian Bogor,Bogor 16680, Jawa Barat, Indonesia.(3) Balai Penelitian dan Pengembangan Budidaya Air Tawar, Jl. SempurNo.1, Bogor 16154, Jawa Barat, Indonesia

Haliotis asinina merupakan salah satu spesies abalon tropisdi perairan Indonesia yang bernilai ekonomis danberpotensi untuk usaha budidaya. Namun informasimendasar tentang identitas dan keragaman genetik spesiesini di alam belum banyak diketahui. Penelitian inibertujuan untuk mendapatkan identitas spesies, jarakgenetik dan keragaman haplotipe antar populasi H. asininadi perairan Indonesia yang meliputi Pulau Kelapa, PulauBira, Pulau Raas Madura dan Pulau Naeng Sulawesimenggunakan metode Polymerase Chain Reaction (PCR).Hasil penelitian menunjukan identitas spesies berdasarkanBLAST nukleotida dari 10 runutan gen COI parsialsepanjang 580 pasang basa (pb) diperoleh persentaseidentity spesies sebesar 99% dan Query cover berkisar 96 –98%. Rata-rata jarak genetik antar populasi 1,17%.Keragaman haplotipe menunjukkan terdapat empathaplotipe H. asinina diantaranya haplotipe 1 asal PulauKelapa (Kep. Seribu); haplotipe 2 asal Pulau Bira (Kep.Seribu); haplotipe 3 asal Pulau Raas Madura; haplotipe 4asal pulau Naeng Sulawesi dengan keragaman rata-rata0,836. Analisis network antar haplotipe dan konstruksiulang pohon filogenetik antar populasi H. asinina diperairan indonesia terbagi atas dua kelompok utama yaituperairan Indonesia bagian barat (Pulau kelapa dan PulauBira) dan perairan Indonesia bagian timur (Pulau RaasMadura dan Pulau Naeng Sulawesi).

COI, Haliotis asinina, keragaman genetik, perairanIndonesia

AO-05Masukan jumlah energi satuan panas terhadapkualitas hasil panen babycorn (Zea mays) didataran rendahIndra DwipaJurusan Budidaya Pertanian, Fakultas Pertanian, Universitas Andalas.Kampus Unand Limau Manih, Padang 25163, Sumatera Barat. Tel. +62-751-72773, Fax.: +62-751-72702, email: [email protected]

Penggunaan satuan panas panen merupakan salah satu carauntuk mendapatkan kualitas hasil babycorn (Zea mays L.)yang baik. Penelitian dilaksanakan di lapangan, KelurahanKuranji, Kecamatan Kuranji Kota Padang pada ketinggianlahan ± 25 mdpl dari bulan Agustus sampai Desember2015. Penelitian ini bertujuan untuk mengetahui jumlahmasukan energi satuan panas yang tepat untukmenghasilkan tanaman babycorn yang berkualitas baik.Rancangan Acak Kelompok (RAK) dengan 5 perlakuandan 4 ulangan digunakan dalam penelitian ini denganmasing masing-masing perlakuan: A (710-730 SP), B (740-760 SP), C (770-790 SP), D (800-820 SP) dan E (830-850SP). Data hasil pengamatan dinalisis dengan uji F dandilanjutkan dengan Uji Duncan’s New Multiple Range(DNMRT) pada taraf nyata 5%. Hasil penelitian

ABS MASY BIODIV INDON 4 (1): 1-18, Depok, 28-29 January 2017 3

menunjukkan bahwa panen dengan satuan panas 830-850SP memberikan hasil terbaik terhadap diameter tongkol,panjang tongkol dan kadar serat.

Babycorn, panen Satuan Panas, Zea mays,

AO-06New polymorphisms of exon 8 of the GrowthHormone Receptor Gene in Pesisir CattleYurnalis1, ♥, Arnim2, Sarbaini Anwar1, Dino Eka Putra1

1 Department of Genetic and Animal Breeding, Faculty of Animal Science,Universitas Andalas. Jl. Prof. Dr. Hamka Kampus Unand. Limau Manis,Padang 25163, West Sumatra, Indonesia. Tel.: +62-751-71464 ext 617,Fax.: +62-751-71464, ♥email: [email protected] Department of Animal Production, Faculty of Animal Science,Universitas Andalas. Jl. Prof. Dr. Hamka Kampus Unand. Limau Manis,Padang 25163, West Sumatra, Indonesia

Growth hormone (GH) exerts its effects on growth andmetabolism by interacting with a specific receptor (GHR)on the surface of the target cells. Therefore, GHR has beensuggested as candidate gene for growth traits in cattle. Thepurposed of this study were to identify geneticpolymorphism of the Growth Hormone Receptor (GHR)genes and its relation with average daily gain and bodymeasurement in Pesisir Cattle. Total of 176 blood sampleswere collected from two populations belonging to PadangMangatas Breeding Station (23 samples) and from PesisirSelatan district (153 samples). Genomic DNAs wereextracted by Promega DNA purification kit and amplifiedby a polymerase chain reaction (PCR) techniques then PCRproducts were direct sequenced to detect polymorphismand then were genotyped by the Polymerase ChainReaction-Restriction Fragment Length Polymorphism(PCR-RFLP) methods using two enzyme Tas1 and Tsca1.For GHR/Tas1 there were two allele dan three genotypeswere found namely: allele A and B with A frequency 0.42relatively higher than allele B frequency 0.58 andgenotypes AA, AB, and BB with frequency genotype were0.17 , 0.50, and 0.33 respectively. For GHR/Tsca1 therewere two allele and two genotypes were found namely:allele C and D with A frequency 0.61 relatively higher thanallele B frequency 0.39 and genotypes CC and CD withfrequency genotypes 0.22 and 0.78 respectively. These twopolymorphism were not significant for average daily gainand body measurement as body length (BL), height atwithers (WH) andchest depth (CD)

Body measurement, GHR, growth, Pesisir cattle

AO-07Polimorfisme gen reseptor hormon pertumbuhan(GHR) pada sapi Pasundan, Jawa BaratWidya Pintaka Bayu Putra♥, Paskah Partogi Agung,Saiful Anwar, Syahruddin Said

Pusat Penelitian Bioteknologi, Lembaga Ilmu Pengetahuan Indonesia.Cibinong Science Center, Jl. Raya Bogor Km 46 Cibinong-Bogor 16911,West Java, Indonesia. Tel. +62-21-8754587, Fax. +62-21-8754588,♥email: [email protected]

Identifikasi polimorfisme pada gen yang mengontrol sifatpertumbuhan berguna untuk melakukan seleksi ternaksecara molekuler. Salah satu gen yang mengontrol sifatpertumbuhan adalah gen reseptor hormon pertumbuhan(GHR) yang telah banyak digunakan sebagai marka genetikdalam meningkatkan produksi susu dan berat badan padabeberapa bangsa sapi. Penelitian ini bertujuan untukmengidentifikasi keragaman gen GHR pada sapi Pasundan.Penelitian ini menggunakan 69 sampel DNA sapi Pasundandari BPPT-SP Ciamis, Jawa Barat. Metode analisis DNAyang digunakan adalah PCR-RFLP dengan enzim restriksiAluI (AG*CT). Hasil penelitian menunjukkan bahwa genGHR (298 pb) pada sapi Pasundan bersifat polimorfikdengan tiga tipe genotip yaitu: AA (0,41); AG (0,41) danGG (0,18). Frekuensi allel A dan G masing-masing sebesar0,61 dan 0,39. Jumlah allel efektif (Ne) sebesar 1,91 dantidak menunjukkan efek terfixsasi (Fis<1,00). Hasil uji Chisquare (χ2) menunjukkan bahwa frekuensi genotip padasampel populasi yang diuji masih dalam keseimbanganHardy-Weinberg (HWE). Nilai heterozigositas harapan(He) dan observasi (Ho) memiliki nilai yang sama (0,48).Tingkat informatif penanda genetik (PIC) yang diperolehsebesar 0,36 (moderate). Tingkat keragaman allel antarindividu pada sampel populasi yang diuji termasuk rendah(H<1,00). Kesimpulan dari penelitian ini adalah gen GHRpada sapi Pasundan di BPPT-SP Ciamis bersifat polimorfiksehingga memungkinkan untuk digunakan sebagai seleksimolekuler melalui kajian yang lebih dalam lagi.Penambahan jumlah sampel dari beberapa wilayah populasisapi Pasundan akan memberikan informasi yang lebihakurat mengenai keragaman genetik GHR pada sapiPasundan di Jawa Barat.

Gen GHR, PCR-RFLP, polimorfisme, sapi Pasundan

AO-08Keragaman gen growth hormone (GH) padabeberapa rumpun sapi lokal IndonesiaPaskah Partogi Agung♥, Saiful Anwar, Widya PintakaBayu Putra, Dan Syahruddin SaidPusat Penelitian Bioteknologi, Lembaga Ilmu Pengetahuan Indonesia.Cibinong Science Center, Jl. Raya Bogor Km 46 Cibinong-Bogor 16911,West Java, Indonesia. Tel. +62-21-8754587, Fax. +62-21-8754588,♥email: [email protected]

Melalui kajian ilmu genetika molekuler, individu yangberpotensi genetik unggul dalam suatu kelompok sapidapat dideteksi lebih dini sehingga dapat digunakansebagai calon pejantan maupun induk untuk meningkatkanproduktivitas sapi potong lokal. Gen GH (GrowthHormone) menjadi salah satu gen yang mempengaruhiproduktivitas ternak seperti sifat pertumbuhan danreproduksi. Gen GH sering dijadikan sebagai kandidat genpada program MAS (Marker Assisted Selection) yaitu


seleksi ternak dengan bantuan teknologi marker genetikuntuk sifat pertumbuhan dan karkas pada sapi. Penelitianini bertujuan untuk mengidentifikasi keragaman gen GHpada beberapa rumpun sapi lokal. Beberapa rumpun sapilokal yang digunakan dalam penelitian ini antara lainrumpun sapi SO, PO, Bali, Pesisir dan Simmental SumateraBarat yang berturut-turut sebanyak 41, 24, 19, 8, dan 31sampel. Metode pendeteksian keragaman gen GH yangdigunakan adalah metode PCR-RFLP menggunakan enzimrestriksi MspI. Hasil penelitian menunjukkan bahwa padasapi SO, PO, dan Pesisir ditemukan tiga genotipe (AA, BBdan AB), sapi Simmental Sumatera Barat dua genotipe (BBdan AB), sedangkan sapi Bali hanya satu genotipe (AA).Berdasarkan hasil tersebut dapat disimpulkan bahwa genGH pada sapi SO, PO, Pesisir dan Simmental SumateraBarat dalam kondisi beragam (polimorfik) sedangkan padasapi Bali tidak beragam (monomorfik). Informasi dalampenelitian ini dapat dijadikan dasar atau landasan dalammemulai program seleksi dan perkawinan sapi potong lokalIndonesia berbasis teknologi molekuler.

Gen GH, Indonesia, keragaman, sapi lokal

AO-09Moleceular phylogeny of Eastern SumatranSpecies of Mangifera (Anacardiaceae) within thegenus Inferred from ITS SequenceIbna Hayati♥, Fitmawati, Nery SofiyantiDepartment of Biology, Faculty of Mathematics and Natural Sciences,Universitas Riau. Kampus Bina Widya Jl. H.R. Subrantas KM 12,5,Pekanbaru 28293, Riau. Tel. +62-761-65593. ♥email:[email protected]

The phylogenetic relationships of the genus Mangiferawith emphasis on eastern Sumatran species were estimatedusing sequence data from internal transcribed spacer (ITS)regions of nrDNA. The separation of the two traditionallyaccepted subgenera was not well supported. Themonophyly of each section of Deciduae, Perennes,Marchandora, Euantherae, Rawa, and Mangifera withingenus remained unclear. To clarify the phylogeneticrelationship, we examined four different methods ofphylogenetic tree reconstruction with the addition ofoutgroups and sequences of Mangifera from the NCBIGenBank as representative species of each section. Theresulting tree topologies were mostly consistent with oneanother. Judging from the phylogenetic tree some ofsection should be fused. We also provide the moleculardescription for the common mango species found inSumatra and create comparison among them. The resultwas support the resurrection of species Mangiferasumatrana Miq. Nucleotide variation among Mangiferaspecies should be interpreted as modification ofenvironment. Molecular data support placement of specieswithin the genus.

Eastern Sumatra, ITS, Mangifera, molecular descriptionPhylogenetic relationship

AO-10Anti-Mycobacterium tuberculosis Strain H37Rvactivity against Brazilin Compound In VitroRatu Safitri 1, 2,♥, Aya Sofa Novia W1, Mas Rizky A.A.Syamsunarno2,3, Ani Melani Maskoen3

1 Departement of Biology, Faculty of Mathematics and Natural Sciences,Universitas Padjadjaran. Jl. Bandung-Sumedang Km 21 Jatinangor,Sumedang 45363, West Java, Indonesia.Tel./Fax. + 62-22-77964, ♥email:[email protected] Central Laboratory of Universitas Padjadjaran Jl. Bandung-Sumedang Km 21 Jatinangor, Sumedang, 45363, West Java, Indonesia.3 Faculty Of Medicine, Universitas Padjadjaran Jl. Raya Bandung-Sumedang KM 21, Jatinangor 45363. Sumedang West Java, Indonesia

Brazilin compound is known to have capability againstseveral pathogenic bacteria, but until now, the ability ofbrazilin as antituberculosis haven’t been investigated yet.Based on some researches, it’s known that brazilin iscapable to chelate iron. Mtb’s growth also stronglyinfluenced by the presence of iron. The purpose of thisstudy was to assess the potential of anti Mtb from brazilin.This research was conducted by examining the minimuminhibitory concentration (MIC) and minimum bactericidalconcentration (MBC). The MIC and MBC tests usedproportion method consisting 4 treatment groups, namelythe positive control (Lownstein-Jensen medium inoculatedwith Mtb), the negative control (LJ medium), the anti TBdrugs (rifampicin, isoniazid, ethambutol and streptomycin),and brazilin (1, 2, 4, 8, 16, 32, 64, 128, 256, 512, and 1024ppm) that were observed for 8 weeks. The capability ofbrazilin compound to chelate iron in 4 different groups wasassessed using Atomic Absorption Spectrophotometer(AAS) method. The test results showed that the MIC ofbrazilin presented at 128 ppm, while the MBC presented at256 ppm. The results also showed that brazilin compoundat 128 ppm concentration was able to chelate iron up to32.96% and reduce the growth of Mtb up to 72% in 10-3Mtb dilution and up to 60% in 10-5 Mtb dilution. Theantituberculosis potential of brazilin is suspected becauseof its ability to chelate iron and the antimicrobial propertiesof brazilin structure itself.

Brazilin, iron chelation MBC, MIC, Mycobacteriumtuberculosis

Diversity of Species

BO-01Application of Open Source Software ImageJ formorphometrical measurements of of batsspermatozoa Genus CynopterusTaufiq Purna Nugraha1, ♥, Iman Supriatna,2 SrihadiAgungpriyono3, Gono Semiadi4


1 Pusat Penelitian Biologi, Lembaga Ilmu Pengetahuan Indonesia.Cibinong Science Center, Jl. Raya Bogor Km 46 Cibinong, Bogor 16911,West Java, Indonesia. Tel.: +62-21-876156, Fax. +62-218765062, ♥email:[email protected] Bagian Reproduksi dan Kebidanan, Departemen Klinik, Reproduksi danPatologi,Fakultas Kedokteran Hewan, Institut Pertanian Bogor, KampusIPB Darmaga Bogor 16680, Indonesia3 Bagian Histologi, Departemen Anatomi, Fisiologi dan Farmakologi ,Fakultas Kedokteran Hewan, Institut Pertanian Bogor, Kampus IPBDarmaga Bogor 16680, Indonesia4 Pusat Penelitian Biologi, Lembaga Ilmu Pengetahuan Indonesia, Jl RayaCibinong Km. 46, Cibinong, Bogor –Indonesia

The morphology in male reproductive system is one of themost striking and widespread patterns in the evolutionaryradiation of animal taxis. Features such as anatomy of glanspenis, baculum, accessories glands, sperm morphometryand structure of penile spine is known as a reproductivebarrier between species that and is known to be useful intaxonomic studies. This study was conducted todifferentiate species-level boundaries within theCynopterus trough the analysis of sperm morphometry.Morphometry of spermatozoa cell from 7 species withinthe genus Cynopterus were measured using open sourcesoftware ImageJ. The results showed that C. nusatenggarahave the most distinctive sperm morphometricalmeasurement compared with other Cynopterus, with thebiggest head area (28.59 ± 1.95 µm2) and the shortestmidpiece and tail length (22.62 ± 1.35 µm; 87.19 ±3.25µm) while C. minutus has the smallest head area (24.24 ±1.83 µm), C. horsfieldii has the longest midpiece (25.57 ±1.03 µm) and C. luzoniensis has the longest tail (98.13 ±3.10 µm). ImageJ has shown to be reliable to assistmorphometrical measurement of spermatozoa, its opennessand free of use is giving and advantage compared toproprietary Computer Assisted Sperm Analysis Program.

Comparative sperm morphometry, Cynopterus, spermatozoa,ImageJ

BO-02Keanekaragaman jenis serta distribusi populasimammalia arboreal di kawasan Hutan Kemuning,Bejen, Kabupaten Temanggung, Jawa TengahAgnes Audina Krisanti1, ♥, Tetri Widiyani1, M. AliImron2

1Jurusan Biologi, Fakultas Matematika dan Ilmu Pengetahuan Alam,Universitas Sebelas Maret. Ir. Sutami, No. 36A, Kentingan, Surakarta57126, Jawa Tengah. Tel. +62-271-669124. ♥email:[email protected] Fakultas Kehutanan, Universitas Gadjah Mada. Yogyakarta 55281,Indonesia

Mammalia merupakan hewan vertebrata yang mendudukiberagam tingkat trofik, mulai dari konsumen hingga toppredator. Mammalia arboreal yang menghabiskan hampirseluruh waktunya di pepohonan memiliki peran pentingdalam keseimbangan ekosistem dan ketahanan ekosistemhutan. Pengetahuan terkait distribusi dan keanekaragamansuatu bidang kecil dari hutan tropis dataran rendah sepertiHutan Kemuning masih jarang dijumpai. Tujuan dari

penelitian ini adalah untuk mengetahui keanekaragamandan distribusi mammalia arboreal, serta pengguaan habitatoleh mammalia arboreal di Hutan Kemuning, KecamatanBejen, Temanggung, Jawa Tengah. Pengamatan dilakukanterhadap jenis-jenis mammalia arboreal selama 4 minggu,mulai dari bulan April sampai bulan Juli 2016. Pengamatandilakukan dalam waktu yang bervariasi antara pagi, siang,dan/ atau sore. Sebanyak 30 jalur Strip Transect (ST)sepanjang 200m ditentukan untuk mengamati jenis satwa,sedangkan Point-quarter Sampling (PQS) pada setiap 50mjalur transek dilakukan untuk menentukan keragaman,analisis vegetasi, dan kepadatan pohon pada habitat.Sebanyak 137 individu mammalia arboreal yang teramatiterdiri dari 6 spesies, yaitu Callosciurus notatus, Ratufabicolor, Galeopterus variegatus, Macaca fascicularis,Trachypithecus auratus, dan Nycteris javanica. Populasiterbesar ditemukan pada M. fascicularis dan jenis denganpopulasi terkecil adalah G. variegatus. Tegakan yangteramati adalah sebanyak 28 spesies. Satwa teramati pada22 jalur, sedangkan pada 6 jalur lainnya tidak teramatikehadiran satwa sehingga frekuensi kehadiran satwasebesar 80%. Indeks Shanon-Weiner menunjukkan tingkatkeragaman satwa pada kawasan rendah, sedangkankeragaman vegetasi sedang. Spesies tumbuhan yangmendominasi kawasan adalah Ficus macrophylla(INP=36%). Pola distribusi dari tiap jenis menunjukkanpola mengelompok.

Distribusi, Hutan Kemuning, keanekaragaman, mammaliaarboreal

BO-03Diversity of Rhizosphere entomopathogenic fungiof groundnut and its virulence against the podborer, Etiella zinckenella, in West SumatraReflinaldon♥, Hasmiandy Hamid, Trizelia, FaridwanAmir, Nelpi Gusnita, Nova RosaDepartment Plant Protection, Faculty of Agriculture, Universitas Andalas.Kampus Unand Limau Manih, Padang 25163, West Sumatra, Indonesia.Tel. +62-751-72773, Fax.: +62-751-72702, ♥email:[email protected]

Attack of pod borer on groundnut can be controlledeffectively with using of entomopathogenic fungi originatefrom rhizosphere of groundnut planting. This study aims toidentify and study the various species of entomopathogenicfungi in the rhizosphere of groundnut plants were testedagainst the pod borer, Etiella zinckenella in the laboratory.Fungi collection carried out in some central areas of WestSumatra peanut crop in the period 2012 to 2014. Theisolation method is carried out with dilution and usingTenebrio molitor larvae as baiting. All fungal were isolatedtested on the 5th instar larvae of E. zinckenella todetermine the pathogenicity of each. Isolated from therhizosphere soil found in various genera of fungi, namely:Metarhizium, Beauveria, Trichoderma, Aspergillus andFusarium with pathogenicity levels vary from each isolate.Based on the results of this study concluded that


entomopathogenic fungi from the rhizosphere highpotential to be developed as a biological control agent onpeanut pod borer

Beauveria, Metarhizium, patogenicity, peanut, Trichoderma

BO-04In vitro characterization of indigenous Bacillusspp. to control Bacterial Wilt Disease (Ralstoniasolanacearum) and increase growth rateYulmira Yanti1, ♥, Trimurti Habazar1, Reflinaldon1,Chainur Rahman Nasution2, Srimano Felia2

1 Department Plant Protection, Faculty of Agriculture, UniversitasAndalas. Kampus Unand Limau Manih, Padang 25163, West Sumatra,Indonesia. Tel. +62-751-72773, Fax.: +62-751-72702, ♥email:[email protected] Department of Agroecotechnology, Faculty of Agriculture, UniversitasAndalas. Kampus Unand Limau Manih, Padang 25163, West Sumatra,Indonesia

Rhizobacteria from group of Bacillus spp. are the mostpotential genera in development because of its sporeforming ability which could increase the adaptation. Basedon the previous research in planta screening, sixrhizobacterial isolates obtained which can control bacterialwilt disease and increase growth rate of chili had beenidentified. Those are Bacillus subtilis BSn5 (RZ 2.1. AP 3),Bacillus sp. RD-AZPVI-03 (RZ 2.1 AP 1), Bacillus sp.Y81-1 (RZ 2.2 AG 2), Bacillus cereus strain C38/15 (RZ 21 AG 1), Bacillus cereus strain APSB-03 (RA 1.2 AP.1),and Bacillus subtilis strain CIFT MFB 4158A (RZ 1.1AP1). This research aimed to characterized the ability ofpotential Bacillus spp. in vitro to control disease andpromote growth. Characters identified are production ofIndole Acetic Acid (IAA), Ammonia, HCN, siderophore,phosphate solvent, biosurfactant, protease, rootcolonization, and growth suppression of R. solanacearum.All strains had ability to produce variable concentrations ofIAA, B. subtilis strain CIFT MFB 4158A can producesiderophore, all isolates not producing HCN andbiosurfactant, 2 isolates can produce protease. Based onability to colonized roots, B. subtilis strain CIFT MFB4158A had best ability to colonized root surface(4,17 x 105sel/g root), while Bacillus subtilis BSn5 had best ability tocolonized internal roots (endophytic) (2,08 x 105 sel/g),and 2 Bacillus strains can suppress R. solanacearum invitro.

Bacillus, in vitro, Indole Acetic Acid, siderophore, protease

BO-05Keanekaragaman isolat rizobakteria indigenusdan potensinya sebagai induser ketahanan bawangdaun terhadap hama Spodoptera exigua(Lepidoptera: Noctuidae)Trimurti Habazar♥, Arneti, Ilma Juita

Faculty of Agriculture, Universitas Andalas. Kampus Unand LimauManih, Padang 25163, West Sumatra, Indonesia. Tel. +62-751-72773,Fax.: +62-751-72702, ♥ email: [email protected]

Rhizobakteria berperanan umumnya untuk pengendalianberbagai jenis penyakit tanaman, namun akhir-akhir inijuga sudah dilaporkan untuk pengendalian hama tanamanterutama serangga. Penelitian ini bertujuan untukmengetahui keanekaragaman isolat rizobakteria daririzosfer bawang daun dan kemampuannya meningkatkanketahanan tanaman bawang daun terhadap seranganSpodoptera exigua. Penelitian terdiri atas 3 tahap, yaitu: 1)eksplorasi dan karakterisasi keanekaragaman isolatrhizobakteria di daerah endemik S. exigua. 2) Seleksi isolatrizobakteria indigenos untuk pemacu pertumbuhan bawangmerah (PGPR). Pada tahap ini penelitian dalam bentukpercobaan yang dirancang secara acak lengkap dengan 16perlakuan (15 isolat Rizobakteria dan kontrol). dan 3ulangan. 3) Seleksi isolat rizobakteria indigenos untukpengendalian S. exigua pada tanaman bawang daun.Rancangan percobaan sama dengan tahap 2, yang terdiriatas 14 perlakuan {12 isolat Rhizobakteria terpilih (hasiltahap 1), insektisida dan kontrol} dan 3 ulangan. Peubahyang diamati adalah saat mulai serangan S. exigua, jumlahkelompok telur, persentase dan intensitas serangan padabawang daun. Hasil penelitian menunjukkan bahwa 15isolat rhizobakteria dari rhizosfer bawang daun tegolongberanekaragam yang terdiri atas 12 tipe koloni, sebagianbesar tergolong Gram (+) dan semuanya bersifat reaksihipersensitif negatif atau bukan patogen tanaman. Sebagianbesar isolat rizobakteria mampu memacu pertumbuhanbawang merah dan yang terbaik adalah AGRZ 14danAGRZ 21. Demikian juga untuk peningkatan ketahananbawang daun terhadap S. exigua, isolat rhizobakteria yangterbaik yaitu: AGRZ 21, AGRZ 43, AGRZ 11, AGRZ 14,AGRZ 13 dan AGRZ 21.

Bawang daun, induksi ketahanan, keanekargaman,rhizobakteria, Spodoptera exigua.

BO-06Variasi musiman keragaman dan distribusifungi mikoriza arbuskula di areal pertanamansawitDelvianFakultas Kehutanan, Universitas Sumatera Utara. Jl. Tridharma Ujung No.1, Kampus USU Medan20155, Sumatera Utara, Indonesia. Tel./Fax.: +62-061-8220506. ♥email: [email protected]

Penelitian ini bertujuan untuk mengetahui keragaman dandistribusi fungi mikoriza arbuskula pada rizosfirpertanaman sawit di beberapa areal perkebunan rakyat diKabupaten Deli Serdang, Sumatera Utara. Eksplorasi FMAdilakukan tiga kali dalam waktu yang berbeda. Hasilpenelitian menunjukkan adanya variasi kepadatan sporaFMA, persentase kolonisasi akar dan jumlah jenis FMAyang ditemukan pada setiap waktu sampling yang berbeda.Kepadatan spora dan jumlah jenis FMA terbanyak


diperoleh pada periode di mana curah hujan relatif rendah(Agustus), sedangkan persentase kolonisasi tertinggi terjadipada saat curah hujan tinggi (November). Jenis FMA yangditemukan sebanyak tiga genus yaitu, Glomus, Gigasporadan Acaulospra. Genus Glomus menghasilkan jumlahspora terbanyak baik pada lokasi maupun waktu samplingyang berbeda. Glomus juga mempunyai jumlah jenis yanglebih banyak daripada Gigaspora dan Acaulospora.

Jumlah spora, kolonisasi, variasi musiman, mikorizaarbuskula, sawit

BO-07Conservation of biodiversity aquatic plants fromBorneo, Indonesia through tissue culturetechniques for free pathogen aquarium scapeMedia Fitri Isma Nugraha1,♥, Rosa Yunita1, EndangGati Lestari2, Anjang Bangun Prasetio2

1Balai Penelitian dan Pengembangan Budidaya Ikan Hias. Jl PerikananNo. 13 Pancoran Mas, Depok 16436, Jawa Barat. ♥email: [email protected] Balai Besar Bioteknologi dan Sumberdaya Genetik Pertanian Bogor. Jl .Tentara Pelajar No. 3A Bogor 16111, Jawa Barat

Indonesia is a big mega biodiversity countries in the world.One of which Indonesian biodiversity very famous isaquatic plant. Many Indonesian aquatic plants speciesunkwon but their existence has threatened. Bucephalandraspecies is one of endemic species aquatic plants fromBorneo. These species is known as a genus semi aquaticflowering plants. Many of aquatic plants geeks in the worldhunt Bucephalandra species. Accordingly the sale price ofBucephalandra species very fantastic (US$ 50 / rhizome).Until now Bucephalandra sp., has been explorated in theirhabitat and sold in kilogram. Population this species hasbeen cutback in their natural habitat. Rescue of endemicspecies is very importance. The aim this study is tomultiplication Bucephalandra sp. for rescue andconservation through tissue culture technique, to supportthe marketing of aquatic plant without damaging naturalecosystems and pathogen-free. Mehode of this study are,we use based tissue culture medium (Murashe and skoog,1962) / medium MS, with sterilisation and growth regulatorthat our formulation. the results of this study is,Bucephalandra sp., are aquatic plant that can multiply withtissue culture technique using MS basic medium byproviding new modifications on sterilization techniquesand plant growth regulators.

Bucephalandra, conservation, Endemic Borneo, tissueculture

BO-08The preferences of lobster fishermen to catchingthe Painted Spiny Lobster (Panulirus versicolorLattreille, 1804) in South Coast of Java, Indonesia

Hanum IsfaeniDepartment of Biology, Faculty of Mathematics and Natural Sciences,Universitas Negeri Jakarta. Jl. Pemuda No. 10, Rawamangun, JakartaTimur. ♥email: [email protected]

The Painted spiny lobster (Panulirus versicolor Lattreille,1804) is one the most economical or commercial lobsterafter the ornate lobster in the lobster fishermen or trader inIndonesia. The research conducted to investigate thepreference of fishermen to get this painted spiny lobster.The research worked in a descriptive method throughsurvey technique. The data was collected by interview anddirect observations. We used the chi square test toanalyzing the data. The result show there was notdifferences between any lobster fishing locations to catchthe painted spiny lobster. The fishermen catching of thespiny lobster species was not differences in the South Coastof Java Island.

Panulirus versicolor, preference, South Coast, spiny lobster

BO-09Keanekaragaman tumbuhan tropis berpotensisebagai atraktan alami untuk pengendalian rayapdengan sistem umpanYuliati Indrayani1,♥, Musrizal Muin2, TsuyoshiYoshimura3

1 Faculty of Forestry, Universitas Tanjungpura. Jl. Prof. Hadari Nawawi,Pontianak 78121, West Kalimantan, Indonesia. Tel.: +62-561-765342,583865, 732500, Fax.: +62-561-765342, ♥email:[email protected] Faculty of Forestry Hasanuddin University, Jl. Perintis KemerdekaanKM 10 Makassar 90245, Indonesia3 Research Institute for Sustainable Humanosphere, Kyoto University,Gokasho, Uji, Kyoto 611-0011, Japan

Attractants have been used in termite baiting techniquesusually applied for bait-toxicant against subterraneantermites in buildings. This study was conducted to examinethe effectiveness of bio-based attractant on subterraneantermites Coptotermes curvignatus Holmgren underlaboratory conditions. Leaves of five plant species [clove(Syzygium aromaticum), cajuput (Melaleuca bracteata L.),cinnamon (Cinnamomum burmannii), basil (Ocimumsanctum L.), Bay (Syzygium polyanthum Wight)] wereseparately served as termite feeding bio-based attractantswith two different concentrations of 10% and 50%. Fiveworkers and five soldiers of the termites were tested in no-choice test bioassay arenas to determine their preferenceson the bio-based attractants. No-choice bioassay arenaswere assembled by connecting two clear plastic glasses(bottom diameter 5 cm, upper diameter 7 cm) with clearplastic hoses (inner diameter 3 mm, outer diameter 5 mm)to allow the termite movement. Results showed that theresponse of termites on clove leaf-based attractant wasgreater compared to other attractant types as indicated bytermite moving time. The effect of different concentrationsof bio-based attractants to the termite response was notstatistically significant. No direct relationships were noted


between speed of termite moving time and number oftermites recruited to attractants.

Bio-based attractants, diversity spesies, plant leaves, baitingsystem, subterranean termite

BP-01Spatial distribution of abundant tree species at amixed dipterocarps forest in Bukit Bangkirai, EastKalimantan three years after long drought andforest fireMustaid SiregarCenter for Plant Conservation-Bogor Botanic Gardens, IndonesianInstitute of Sciences. Jl. Ir. H.Juanda No. 13, P.O. Box 309, Bogor 16003,West Java, Indonesia. Tel./fax.: +62-251-8322187. ♥email:[email protected]

Spatial distribution of abundant tree species three yearsafter long drought and forest fire in three permanent plotsat Bukit Bangkirai, East Kalimantan was analyzed by usingMorisita’s indices of dispersion, Jaccard’s indices ofspecies association and correlation of tree density withground height and slope in each plots. The results showedthat most of the abundant tree species were clumped atsmall scales, and the clumps were randomly distributed(inter-clump distribution is random). An exception wasfound for Madhuca kingiana at lightly damaged forest dueto forest fires (LD-plot) which have random distribution.Individuals of M. kingiana in natural unburnt forest (K-plot) were clumped at larger scales, and they weredistributed uniformly within the clumps (intra-clumpdistribution is uniform), which were distributed in valleyand the sub-plots with steep slope facing southward.Inversely, mostly Shorea laevis and Macarangaglaberrimus were distributed almost over the entire sub-plots except in valley and the steep slope facing southeast.There is a positive correlation between ground height withtree density for S. laevis and M. glaberrimus, whereas forM. kingiana has a negative correlation, where it is moreabundant in the valley. At heavily damaged forest due toforest fires (HD-plot), distributions of Macarangagigantea, Homalanthus populneus and Mallotuspaniculatus were concentrated at the valley and in thelower parts of the slopes. They are spread out in the opencanopy at the valley which have water availability. Thesethree species were light demanding species and recruitedafter the fires.

Abundant species, forest fire, Bukit Bangkirai, EastKalimantan, spatial distribution

BP-02Variability in abundance and diversity of copepods inMond River estuary, Bushehr, Persian Gulf

Aliakbar Hedayati1,♥, Mojtaba Pouladi1, AmirVazirizadeh2, Amir Qadermarzi1, Ahmadreza Jabaleh1

1Department of Fisheries, Faculty of Fisheries and EnvironmentalSciences, Agriculture and Natural Resources University of Gorgan,Golestan, Iran, Tel.: +98-913 152 8572, ♥email:[email protected] of Marine Biology and Fisheries, Persian Gulf ResearchInstitute, Persian Gulf University, Bushehr, Iran

The present study was carried out to investigate theabundance and biodiversity of copepod from Mond Riverestuary, Bushehr, Persian Gulf during 4 seasons and itsrelationships with environmental parameters. The watersamples were collected in mid-season from spring 2012 towinter 2013 for one year period. Copepod samples werecollected by using of 140 µm plankton net with 25 cmmouth diameter and vertical towing in all stations.Copepod assemblages were comprised of 4 orders, 13families and 10 genera. Orders were included; Calanoida,Cyclopoida, Poecilostomatoida, Harpacticoida and Generawere included: Cathocalanus, Acrocalanus, Paracalanus,Subeucalanus, Centropages, Temora, Calanopia,Labidocera, Pontella, Acartia, Oithona, Oncaea,Corycaeus, Microsetella, Microsetella and Euterpina. Theorder Calanoida with 10 genera was the most diverse anddominant order between Identified orders. The means(±SE) of copepod abundance were recorded in spring(5853.4±1826.19 Individual m-3), in summer(6707.6±1930.15 Ind. m-3), in fall (4393.6±1263.37 Ind. m-

3) and in winter (3400±763.98 Ind. m-3), respectively.Simpson and Shannon-Wiener biodiversity indices wereobtained in spring (0.87±0.11 and 2.86±0.19), in summer(0.89±0.006 and 3.11±0.15), in fall (0.86±0.13 and2.66±0.2) and in winter (0.83±0.12 and 2.39±0.15),respectively. The copepod assemblages had most amountsof diversity and abundance at station 5 in all seasons.Pearson correlation showed significant correlation betweencopepod diversity and abundance with salinity, pH andtemperature. Results showed salinity factor was moreeffective environmental factor on Simpson (0.01> P andr=0.783) and Shannon-Weiner indices (0.01> P andr=0.727), and copepod abundance (0.01> P and r=0.664) inMR estuary.

Copepod, abundance, diversity, environmental factor, Mondestuary

BP-03Production and characterization of a neutralphytase of Penicillium oxalicum strain EUFR-3from Himalayan regionRamandeep Kaur1, Abhishake Saxena1, PuneshSangwan2, Ajar Nath Yadav1, Vinod Kumar1,♥,Harcharan Singh Dhaliwal1

1Department of Biotechnology, Akal College of Agriculture, EternalUniversity, Baru Sahib-173101, India. Tel: +91-1799-276012; Fax: +91-1799-276006, ♥email: [email protected] of Biochemistry, Akal College of Basic Sciences, EternalUniversity, Baru Sahib-173101, India


Cereals are major staple food crops in developingcountries. These have insufficient levels of micronutrientsin seeds to fulfill the recommended dietary allowance.However, the bioavailability of existing levels ofmicronutrients for monogastric animals is low because ofanti-nutrients like phytic acid in cereals which can chelatedivalent cations. Among several approaches of increasingmicronutrient bioavailability, addition of exogenousphytase to cereals based foods during processing canimprove its nutritional value. In the present investigation,40 fungal isolates including Aspergillus, Penicillium,Trichoderma, Toliposporium, Cladosporium, Talaromycesetc. from different habitats were screened positive forphytase activity on specific media. On the basis of phytaseproduction potential in solid state fermentation conditions,plant epiphytic fungi Penicillium oxalicum strain EUFR-3has been observed with maximum phytase activity of 12.8U/g. The biochemical properties i.e. activity and stabilityunder optimum pH and temperature, and effect ofmodulators on phytase activity from this isolate werestudied for its prospective application in human food. TheP. oxalicum EUFR-3 phytase (PhyP-EUFR3) hadmaximum activity at pH 7.0 and temperature 40 °C. It wasstable in pH range of 3-8 with more than 60% activitythroughout investigation. The thermostability of PhyP-EUFR3 was about 30% residual activities after 10 min at80°C. Further purification, characterization and study ofcatalytic mechanism will be useful in better utilization ofthis phytase in human food for increased bioavailability ofimportant metal ions.

Phytase, phytate, micronutrient bioavailability, Penicilliumoxalicum

Diversity of Ecosystem

CO-01The estimation potential livestock breeding ofbuffalo in Ulakan Tapakis Sub-district, PadangPariaman District, West Sumatra, IndonesiaDino Eka Putra1, ♥, Sarbaini2, Tinda Afriani2

1Department of Genetic and Animal Breeding, Faculty of Animal Science,Universitas Andalas. Jl. Prof. Dr. Hamka Kampus Unand. Limau Manis,Padang 25163, West Sumatra, Indonesia. Tel.: +62-751-71464 ext 617,Fax.: +62-751-71464, ♥email: [email protected] 2Department ofAnimal Reproduction, Faculty of Animal Science, Universitas Andalas. Jl.Prof. Dr. Hamka Kampus Unand. Limau Manis, Padang 25163, WestSumatra, Indonesia

In the course of the acceleration of national meatproduction contributes buffalo important after meat fromcattle. Buffaloes (Bubalus bubalis) large enough potentialto be developed as cattle meat, therefore the informationabout the ability to reproductive traits at the buffaloes is

necessary so that the potential of the region as a source ofseed for replacement as well as going to be used in cattlecan be preserved and enhanced. This study aimed todetermine the potential of buffaloes in Ulakan Tapakis sub-district to produce seeds and viability as a source buffaloes.Census and questionnaire are used in this study in whichthree sub-district characterized by high, medium and lowbuffalo population are chosen quota sampling. Theobserved variables in this study are the identity of thebreeder and the buffalo. The development of buffalopopulation was analyzed using breeding theory approach.The average increase of the population was analyzed usinga time series analysis. The results showed that NetReplacement Rate (NRR) value in male and female of157,31% and 191,27% and the natural increase (NI) valueof 23,66% indicating a moderate increase of the population.This study also shows that the population of buffaloes inthe region is sufficient to cover its need. Male and femaleseed potential of 4,55% and 5,33% of the population. Onaverage the increase population of buffalo from 2011 to2015 annual of as much as 172,75 head or 16.53%.Estimated population of buffaloes in 2016 and 2020 as in1675 head and 2183 head eith average increase populationas much as 63 head or 3,40%.

Buffalo, output, productivity, seeds

CO-02Economic valuation of whale shark tourism inCendrawasih Bay National Park, Papua, IndonesiaZuzy Anna♥, Dicky Surya SaputraDepartment of Fisheries Socio-Economics, Faculty of Fisheries andMarine Science, Universitas Padjadjaran. Jl. Raya Jatinangor Km 21,Jatinangor Sumedang, Sumedang 40600, West Java, Indonesia. Tel. +62-22-87701519. Fax. +62-22-87701518. ♥email: [email protected]

Whale sharks (Rhincodon typus) agregation in the waters ofCendrawasih Bay, has an impact on improving the marinetourism industry in the region. On the other hands, WhaleShark is one of the species listed in the red list ofthreatened species by the International Union forConservation of Nature (IUCN), the vulnerable status,means that a whale shark populations have been reduced by20% to 50% within 10 years or three generations. Thedecline numbers of whale sharks caused by humanactivities that damage the fish and the habitat, such asfishing and tourism activities. This is due to the lack ofpublic awareness about the function and value of theseresources and its habitat. Whale shark has an inherent valueas marine resources, and has an environmental servicesvalue, in relation to tourism activities. This paper measuresthe economic value and environmental services of thewhale shark and its habitat. Method of Travel Cost is usedto calculate the value of expenditures incurred by bothforeign and local tourists. The study also measured thevalue obtained by tourist operators, the value of fishingactivities, and the value of the habitat through people’sWillingness to Pay (WTP), using Contingent Valuation


method (CVM). The policy implication of this research isthe need for appreciation of the whale sharks value, as wellas its habitat, by managing and developing conservationareas, community capacity building on the understandingof the importance of whale sharks and its conservationarea, figuring out the carrying capacity of whale sharktourism, develop a community based management tools formaintaining conservation areas, including preventingillegal fishing, and developing a community-basedconservation area surveillance systems

Cendrawasih Bay, Economic Valuation, ContingentValuation Methods, travel cost method, Whale shark,tourism

CO-03Influence of planting line width on the incrementof Shorea leprosula and Dryobalanops lanceolata atSelective Logging Line Planting SystemM. Taufan Tirkaamiana1, ♥, Afif Ruchaemi2, M.Sumaryono2

1 Faculty of Agriculture, The University of 17 August 1945. Jl. Ir. H.Juanda 80, Samarinda 75124, East Kalimantan, Indonesia, Tel./Fax. +62-541-743390, ♥email: [email protected] Faculty of Forestry, Mulawarman University, Jl. Ki Hajar Dewantara,Samarinda 75119, East Kalimantan. Indonesia

One effort to establish sustainable forest production inIndonesia is Selective Logging and Line Planting System(TPTJ), that expected increase the productivity of the forestby planting the target species along the planting line. Thisstudy aims to determine the stand growth of Shorealeprosula (S. leprosula) and Dryobalanops lanceolata (D.lanceolata) planted in different planting line width.Data of stand growth and basal area of S. leprosula and D.lanceolata were collected by observing 4 PUPs (permanentsample plots) with the size of 100m x 100m at the blockarea of PT. Balikpapan Forest Industries (PT BFI) in theDistrict of Penajam Paser Utara, East Kalimantan,Indonesia. The results showed that the average of diameterincrement and basal area of S. leprosula at the planting linewidth of 3 meters was 1.47cm/yr and 1.09 m2/ha/yr, S.leprosula at the planting line width of 6 meters was 2.08cm/yr and 0.90 m2/ha/yr, D. lanceolata at the planting linewidth of 3 meters was 0.74 cm/yr and 0.34 m2/ha/yr, D.lanceolata at the planting line width of 6 meters was 1.14cm/yr and 0.33 m2/ha/yr. Acceptance of optimal lightintensity on leaf will accelerate transpiration rate andopening of stomata, thus affecting the rate ofphotosynthesis. The differences of planting line width of 3and 6 meters are very significant on the growth anddiameter increment of S. leprosula stands, but not on basalarea, while D. lanceolata has no significant effect affect onthe growth, diameter increment and basal area.

Dryobalanops lanceolata, light intensity, planting line,Shorea leprosula, silviculture

CO-04Dekomposisi serasah Avicennia alba pada tingkatsalinitas yang berbeda di hutan mangroveKampung Nipah, Sei Nagalawan, Sumatera UtaraKansih Sri Hartini♥, Yunasfi, Media OktariFakultas Kehutanan, Universitas Sumatera Utara. Jl. Tridharma Ujung No.1, Kampus USU Medan20155, Sumatera Utara, Indonesia. Tel./Fax.: +62-061-8220506. ♥ email: [email protected]

Dekomposisi serasah merupakan proses yang pentingdalam siklus hara pada suatu ekosistem. Pada proses iniakan terjadi perubahan baik secara fisik maupun kimia olehmikroorganisme (dekomposer). Penelitian ini bertujuanuntuk menganalisis dekomposisi serasah Avicennia albapada ekosistem hutan mangrove di Kampung Nipah padatingkat salinitas yang brbeda. serta menganalisiskandungan unsur hara karbon, nitrogen dan fosfor.Salinitas dibedakan menjadi 3 zona, yaitu zona 1 dengantingkat salinitas 0-10 ppt, zona 2 dengan tingkat salinitas11-20 ppt, dan zona 3 dengan tingkat salinitas 21-30 ppt.Serasah Avicennia alba yang digunakan sebanyak 18kantong dengan berat masing-masing 50 g, untuk masing-masing tingkat salinitas yang berbeda. Metode yangdigunakan untuk analisis data adalah metode Olson dan ujistatistik. Hasil penelitian menunjukkan bahwa dekomposisiserasah tercepat terjadi pada tingkat salinitas 11-20 ppt.Kandungan unsur hara karbon dan nitrogen tertinggi terjadipada tingkat salinitas 21-30 ppt, sedangkan kandunganfosfor tertinggi berada pada tingkat salinitas 11-20 ppt.

Avicennia alba, dekomposisi, salinitas, serasah

CP-01The population and habitat characteristics ofSunda pangolin (Manis javanica, Desmarest 1822)in the Bojonglarang Jayanti Natural Reserve,West Java, IndonesiaRuhyat Partasasmita1,2,3, ♥, Irina Anidya Mustikasari2,3,Erri Noviar Megantara2,3

1Program Study of Biology, Faculty of Mathematics and Natural Sciences,Universitas Padjajaran. Jl. Raya Bandung-Sumedang Km 21 Jatinangor,Sumedang 45363, West Java, Indonesia. Tel.: +62-22-7797712, ♥email:[email protected] Department of Biology, Faculty of Mathematics and Natural Sciences,Universitas Padjajaran. Jl. Raya Bandung-Sumedang Km 21 Jatinangor,Sumedang 45363, West Java, Indonesia.3 Institute of Ecology (PPSDAL), Universitas Padjajaran. Jl. Sekeloa,Coblong, Bandung City 40134, West Java, Indonesia.

The pangolin is a protected animal, rare and endangeredcloser to extinction (critically endangered), but thepresence of the wild population status is rarely known. Theexistence of the pangolin populations in a habitat can beindicated by the active nest. This is because the pangolin isa nocturnal animal and difficult to find. This study aimed tothe nesting habitat characteristics, population estimates andfactors of interference. Data collection techniques with


semi-structured interviews and sign surveys. The resultsshowed that pangolin populations around 4-16 individuals.The distance between the nearest nests are 2 m, with a nestsite in the range 15-45% slope, around the nest around23.4-30.60C temperature and humidity at the surface of thenest is 81-92%. Pangolin major disruptions in nature in theform of a boar, porcupine and dogs, besides poaching isstill a major factor of population decline.

Bojonglarang Jayanti Natural Reserve, nesting habitat,pangolin, population

CP-02Kandungan nutrien rumput laut asal PantaiBinuangeun, Propinsi BantenLusi Herawati Suryaningrum♥, Reza SamsudinBalai Penelitian dan Pengembangan Budidaya Air Tawar. Jl. Sempur No.1, Sempur, Bogor Tengah, Kota Bogor, Jawa Barat, Indonesia. Tel.: +62-251-313200. ♥email: [email protected]

Rumput laut merupakan sumber daya hayati yangmelimpah di perairan Indonesia. Rumput laut memilikinilai nutrisi yang cukup tinggi sehingga pemanfaatannyasangat beragam. Penelitian ini bertujuan untuk menentukankomposisi nutrien yang terdapat pada rumput laut jenisUlva lactuca, Caulerpa lentillifera, Palmaria palmata,Caulerpa sertularioides, Gracilaria sp. dan Ulva fasciatayang teridentifikasi di Pantai Binuangen, Propinsi Banten.Parameter yang diamati adalah kadar air, protein, lemak,abu, serat kasar dan karbohidrat (BETN). Pengujiandilakukan sebanyak tiga kali ulangan untuk setiapparameter. Kadar air ditentukan melalui pengeringanmenggunakan oven pada suhu 105 C, kadar protein(Nx6,25) ditentukan dengan metode Kjeldahl, lemakmenggunakan ekstraksi soxhlet, abu melalui pengabuanpada suhu 600 C, dan serat kasar ditentukan denganmereaksikan sampel dengan H2SO4 dan NaOH. Hasilpenelitian menunjukkan bahwa rumput laut yangteridentifikasi di pantai Binuangeun memiliki kandunganprotein berkisar 11,11-19,39%, lemak 0,23-0,94%, abu22,73-46,75%, serat kasar 7,66-21,14% dan BETN 19,82-56,93%

Binuangeun, nutrien, rumput laut

CP-03True mangrove species on the remote small islandsof Tambelan, Riau Archipelago Province,IndonesiaAhmad Dwi Setyawan1, ♥, Yaya Ihya Ulumuddin2

1Department of Biology, Faculty of Mathematics and Natural Sciences,Universitas Sebelas Maret University. Jl. Ir. Sutami 36A Surakarta 57126.Tel. +62-271-7994097, Tel. & Fax.: +62-271-663375. ♥email:[email protected]

2Research Center for Oceanography, Indonesian Institute of Sciences(PPO, LIPI), Jl. Pasir Putih I No.1 Ancol Timur, Jakarta Utara 14430,Indonesia

Tambelan Islands consists of 77 small islands, with a totalland area is 169.42 hectares, the sea area is 58993.42hectares, of which 47.82 hectares of mangrove ecosystems,with the largest mangrove area in the Tambelan island(31.35 ha). On the Tambelan Islands, mangrove ecosystemis relatively limited in the absence of major rivers thatsupply the mud and fresh water for the formation of adense mangrove ecosystem. Research by the end of 2010found 17 species of true mangrove, namely Aegicerasfloridum, Avicennia marina, Avicennia officinalis,Bruguiera gymnorrhiza, Bruguiera sexangula, Ceriopstagal, Lumnitzera littorea, Lumnitzera racemosa, Pemphisacidula, Rhizophora apiculata, Rhizophora lamarckii,Rhizophora mucronata, Rhizophora stylosa, Scyphiphorahydrophyllacea, Sonneratia alba, Xylocarpus granatum,and Nypa fruticans.

Mangrove, true mangrove plant, Tambelan

CP-04Biodiversity of endophytic fungifrom macroalgae(Sargassum sp., Gracilaria sp., Gelidium sp., andCaulerpa sp.) at Pari Island, Seribu Islands,JakartaMujizat Kawaroe1,2,♥, Adriani Sunudin2, JunkwanHwangbo3, Ami Shaumi2

1Surfactant and Bioenergy Research Centre, Bogor AgriculturalUniversity. Baranang Siang Campus, Bogor 16143, Indonesia. email:[email protected]; [email protected] of Marine Science and Technology, Faculty of Fisheries andMarine Science, Bogor Agricultural University, Darmaga Campus, Bogor16680, Indonesia3Research Institute of Science and Technology POSCO, Kumho-dong,Gwangyang City, Jeollanam-do, South Korea

In recent study, the potency of endophytic fungi thatisolated from four macroalgae species (Sargassum sp.,Caulerpa sp, Gracilaria sp. and Gelidium sp.) wasexamined with the goal to determine the characteristic andactivity of cellulolitic endophytic fungi. Colonies of fungipurified by colony propagation technique and identified inSEAMEO BIOTROP Laboratory. Cellulolitic activity wastested on CMC (Carboxy Methyl Cellulose) medium,Congo Red Staining, and Miles. A total of 21 isolates fungiwere found and the results showed that selulotic highestindex obtained from Aspergillus westerdijkial (PCLAR 6)in Caulerpa sp. Optimum day of cellulase activity forAspergillus versicolor and Aspergillus sydowii were on day2nd, while Penicillium citrinum and Aspergilluswesterdijkial were on day 1st. SeluloticactivityofAspergillus versicolor and Aspergillus sydowii showedtheir optimum activity at pH 7 phosphate and temperatureat 400C.

Cellulose enzyme, endhophytic, macroalgae, fungi


CP-05Biodiversity of aquatic fauna in mangrove area ofPurworejo District, Central JavaSlamet Mardiyanto Rahayu1,♥, Wiryanto2, Sunarto2

1Bioscience, Postgraduate School, Sebelas Maret University. Jl. Ir. Sutami36A Surakarta 57 126, Central Java, Indonesia. Tel./Fax. +62-271-663375, email: [email protected] of Biology, Department of Biology, Faculty of Mathematics andNatural Sciences, Sebelas Maret University, . Jl. Ir. Sutami 36A Surakarta57 126, Central Java, Indonesia. Tel./Fax. +62-271-663375

Mangroves area have important roles of ecologic, socio-economic, and socio-culture in ecosystem. Mangroves areain Purworejo is run into degradation as impact of illegallogging, fishponds area expansion, settlements expansionand extension of agriculture area. All of the activities willinfluenced the communities structure of mangrovesvegetation and aquatic fauna. The purposes of research is toobserve (i) biodiversity of mangroves vegetation and aquaticfauna; (ii) correlation between mangrove vegetation andaquatic fauna; and (iii) the influence of abiotic factorstowards biotic condition in research sites. This researchconducted in June-September 2016 at 3 station whichdetermined with purposive sampling method. The researchstation is Gedangan (station I), Jatikontal (station II) andNgentak (station III). Vegetation of mangrove and aquaticfauna is observed with methods of line transect andremoval sampling. Association between variables(vegetation and aquatic fauna) is analyzed with PearsonCorrelation Test. Influenced of abiotic factors (physics andchemicals) on biotic (vegetation and aquatic fauna)analyzed with T-Test and Regression Test. According tothe research, 18 species of plants and 34 species of aquaticfauna is observed from mangroves area of Purworejo.Highest value of vegetation diversity is found in station II,specifically in herbs phase and lower plants (ID 1,84)whereas for aquatic fauna in station III (ID 2,50). Pearsoncorrelation test between vegetation and aquatic faunaobtain r value is 0,104. The T Test had determine asignificant difference (sig 0,000) between abiotic factorsand biotic condition which also show the impact of changesin between them. Further test with regression method alsoindicate a weak impact on changes in abiotic factorstowards biotic condition (r2 0,032)

Aquatic fauna, biodiversity, mangrove, vegetation,Purworejo

CP-06Important weeds and their control in soybeanproduction under rice-soybean cropping paternArief Harsono, Suryantini, Siti Muzaiyanah♥

Indonesian Legumes and Tuber Crops Research Institute (ILETRI). Jl.Raya Kendalpayak KM 8, PO BOX 66, Malang 65101, East Java,Indonesia. Tel.: +62-341-801468, Fax. +62-341-801496, ♥email:[email protected]

Weed control is one of technology components in soybeancultivation that absorb high labor and cost. To solve thisproblem, in dry season 2013 we conducted two researcheswith aims to determine the important weeds on soybeanproduction and to obtain the effective weed controls forsoybean at the lowland after rice. The first research wasconducted by survey and the second research wasconducted by planting soybean after rice. The treatmentconsisted of six weed controls i.e : without weed control,twice hand weeding, pre emergence herbicide Oxyfluorfen,Oxyfluorfen + post emergence 2,4-D Dimethil Amida,Oxyfluorfen + once hand weeding, and once hand weeding.The result indicated that the important weeds for soybeanat the lowland after rice were Oriza sativa, Cyperusrotundus, Echinochloa crusgalli, Pilantus niruri, andAgeratum sp. respectively. The weeds can be controlledeffectively by using pre emergence herbicide Oxyfluorfen,or combination of Oxyfluorfen and post emergenceherbicide 2,4-D dimethil amida. These control methodscould reduced the importance weeds value significanly andincreased soybean yield higher than twice weeding.

Weeds, herbicide, soybean, lowland

CP-07Effects of felling intensity on Hymenopterabiodiversity in a natural production forest in EastKalimantan, IndonesiaAhmad Budiaman1, ♥, Noor Farikhah Haneda2, ImaDuwi Robaikah3

1Department of Forest Management, Faculty of Forestry, BogorAgricultural University, Ulin Street, IPB Darmaga Campus, PO BOX 168,Bogor 16680, Telp/Fax. 0251-8621244, ♥email: [email protected] of Silviculture, Faculty of Forestry, Bogor AgriculturalUniversity, Jalan Lingkar Kampus IPB Darmaga, PO BOX 168, Bogor16680.Telp/Fax. 0251-8626806. Email: [email protected] of Forest Management, Faculty of Forestry, BogorAgricultural University, Ulin Street, IPB Darmaga Campus, PO BOX 168,Bogor 16680, Telp/Fax. 0251-8621244, Email: [email protected].

Hymenoptera is one of the major components of insectbiodiversity. Timber harvest, as part of forest management,leads to change the Hymenoptera biodiversity. This studyanalyzes the effects of felling intensity on the Hymenopteradiversity in a production natural forest in East Kalimantan,Indonesia. In this study, the diversity of Hymenoptera wascompared before and after felling in three different fellingintensities (low, moderate and high). The results showedthat the timber felling tend to decrease the abundance ofHymenoptera. Diversity and species richness ofHymenoptera will decline in moderate and high fellingintensity, whereas evenness index of Hymenopteraunchanged at three felling intensities. The composition ofmorphospecies of Hymenoptera was changed after fellingwith high-intensity felling.

Biodiversity, felling intensity, Hymenoptera, productionnatural forest


CP-08The profile of Dipterocarpaceae in MomiwarenProtected Forest in South Manokwari District,West Papua, IndonesiaRima H. S. SiburianDepartmen of Silviculture, Forest Faculty, University of Papua. Jl.GunungSalju Amban Manokwari Papua Barat 98314.email:[email protected]

The Family Dipterocarpaceae is one of the families whichdominated tropical forest in lowland area, but the existenceof this type is very restricted in eastern Indonesia region.Information about Dipterocarpaceae is imperative becausethere are different types of Dipterocarpaceae which havebeen listed in the category of endangered status. Therefore,research is required to obtain data about the potency of thefamily Dipterocarpaceae in West Papua region. Themethod used for collecting data at tree and stake levels wascontinuous strip sampling method while at sapling andseedling levels was line plot sampling method with nestingplot. The data were analyzed to obtain density value,frequency, and important value index (IVI).The result ofthis study found three types of Dipterocarpaceae namelyVatica rassak, Anisopthera thurifera and Hopea iriana.Vatica rassak had the highest IVIat tree level as 14,302 andthe lowest level was the stake level as 9,90.Meanwhile,species H. iriana had INPof 0,176%at the seedling leveland0,114 at sapling level. Tree and stake levels were not foundto this species. Based on stratification type, Vatica rassakhad B stratification with the height between 20 and 30m.

Potency, Dipterocarpaceae, Vatica rassak, Anisoptherathurifera, Hopea iriana

CP-09Effect of Italian Cypress (Cupressus sempervirensvar. horizontalis) reforestation on represencenative tree species in Hyrcanian forest (North ofIran)Leila Vatani1, Seyed Moseh Hosseini 2,♥, MoslemAkbarinia3, Saeed Shamsi4

1 Ph. D. Candidate in Natural Resources Faculty of Tarbiat ModaresUniversity of Iran.2 Professor of Natural Resources Faculty of Tarbiat Modares University ofIran. [email protected] (corresponding author)3 Associate Professor of Natural Resources Faculty of Tarbiat ModaresUniversity of Iran.4 Staff of Mazandaran Wood and Paper Factory

Effects of 15 years old Italian cypress reforestation onrepresented native tree species in Hyrcanian forest (Northof Iran). Three 15 years old Italian cypress reforestationareas and an area of natural broad-leaved deciduous foreststand were selected as studied areas in the downstreamforests of Mazandaran Province. Three plots (20×20 m)were chosen in each studied area and the type of all woodyspecies were recorded as well as their abundance were

counted. Species diversity was calculated by usingShannon-Wiener diversity index. The evenness andrichness indexes were calculated by using Simpson andMenhenic indexes, respectively. Sixteen tree and shrubspecies were appeared in Italian cypress understorynaturally, of which, some species were rare. There was nosignificant difference between the rate of diversity andrichness index of Italian cypress afforestation and naturalbroad-leaved deciduous forest stand. From the point ofview of the rate of evenness, only in one area, a significantdifference was observed between Italian cypressafforestation areas and natural forest stand. Regarding tothe results of this study, Italian cypress afforestation did notreduce the woody plant biodiversity until the age of fifteenyears old.

Biodiversity, woody species, richness, reforestation,Hyrcanian forest

CP-10The pardox of exotic versus indigenous chickenpopulation dynamics and distribution pattern inEthiopia: ReviewKefyalew Alemayehu♥, Tilahun SisayDepartment of Animal Production and Technology, College ofAgriculture and Environmental Sciences, Bahir Dar University. Bahir Dar,Ethiopia. ♥email: [email protected]

Chicken in Ethiopia represents a significant part of thenational economy and contributes to 98.5% and 99.2% ofthe national egg and chicken meat production, respectively.The total chicken population is estimated to be 56.87million of which 95.86, 2.79 and 1.35% are indigenous,hybrid and exotic breeds, respectively. Therefore, theobjective of this paper was to quantify the populationdynamics and distribution patters of exotic versusindigenous chicken population in the country. Differentexotic chicken breeds have been introduced for upgradingof the potential of chicken ecotypes but, the survival,productivity and population size of either exotic orcrossbred is too low. The production and reproductive traitsof local chicken are characterized with small sized eggs,slow growth rate, late maturity, slow age at first mating,small clutch size, natural learning to broodiness and highmortality of chicks. To alleviate these, the Amhara regionalstate for example did massive introduction of exoticgenotypes (Isa Brown, Bovans Brown and PotchefstroomKoekoek) via distribution of fertile eggs, day-old chickens,crossbred pullets and exotic cockerels. However, the exoticchicken population size (206 200, 513705 and 17311216exotic, hybrid and indigenous, respectively) and (the meanegg production/hen/year for Isa, Bovans and PotchefstroomKoekoek was 276.1±11.03, 266.32±8.7 and 187.04±13.49,respectively) is too low. This indeed is due to inappropriateproduction system, genotype and management. Therefore,selection of indigenous chicken ecotypes and crossbreedingor upgrading by introduction of cocks, pullets and or fertileeggs of high egg producing strains with appropriate


production system and management in respectiveproduction system would increase production andproductivity.

Chicken, genotypes, population dynamics, productionsystem

Ethnobiology & Socioeconomics

DO-01Inventory and comparison of species of plants inObat Pahit Herb Ancestors Legacy Of EthnicMalay of Lingga, Riau IslandsFitmawati1, ♥, Nery Sofiyanti1, Rodesia Mustika Roza1,Hidayatul Hazimi1, Yulisa Resti Irawan1, DhanielRidho Winata1, Awal Prichatin Kusumo Dewi2

1 Department of Biology, Faculty of Mathematics and Natural Sciences,Universitas Riau. Kampus Bina Widya Jl. H.R. Subrantas KM 12,5,Pekanbaru 28293, Riau. Tel. +62-761-65593. ♥email:[email protected] Balai Besar Penelitian dan Pengembangan Tanaman Obat dan ObatTradisional Kementerian Kesehatan RI. Jl. Raya Lawu No. 11,Tawangmangu, Karanganyar 57792, Jawa Tengah, Indonesia

Lingga Malay is ethnicities in the Lingga regency, RiauIslands. This ethnis still holds strong ethnic ancestralbeliefs, such as the use of medicinal plants. One of the herbthat is still in use until today is the Obat Pahit herb to keepbody in fitness. This research aims to inventerize the kindsof medicinal plants used in ethnic Malays Lingga in ObatPahit herb. This research was conducted using the surveymethod and observation of the Obat Pahit in the packaging.Interviews were conducted with traditional medicinepractitioners (TMP) 5 (five) people POT, basedquestionnaire. The results of the study during the interviewand observation potions in the packaging shows the numberand different kinds of plants on each TMP. Based oninterview result obtained eight kinds of plants from TMPKalan, 28 species of the TMP SP4, 20 species of the TMPLinau, 13 species of the TMP Resun and 29 species of theTMP Musai. While the results of the observation of thepackaging is obtained, TMP Kalan many as three species ofplants, POT sp4 about 13 species of plants, TMP Linau asmany as 15 species of plants, TMP Resun many as ninespecies of plants and TMP Musai as many as 14 types ofplants. The dominant use of medicinal plants have 3 typesnamely Bauhinia semibifida, Roots Penawa and Cnestispalala.

Ethnic Lingga Malay, Obat Pahit herb

DP-01Factors influencing the adoption of rice paddythreshing machine “Powered-Thresher”: A casestudy of Serang District, Banten ProvinceTian Mulyaqin1,♥, Ishii Keiichi2

1 Banten Assessment Institute for Agricultural Technology of BantenProvince (BPTP). Jl. Ciptayasa Km 01 Ciruas-Serang 42182, Banten. Tel.+62-254-281055, Fax. +62-254282507. ♥email:[email protected] Graduate School of Agricultural Science, Tohoku University, Japan

For ensuring national food security, main focus policy ofIndonesia government is not only to increase riceproduction, but also to reduce harvest and post-harvestlosses in order to safe rice production. Introducing anddisseminating threshing machine such as powered-thresherto rice farmer is one of the government efforts to reduceyield losses and labor cost, especially on threshing stage.This paper examined the factors influencing the adoption ofpowered-thresher in rice production area in Serang District,Banten Province, Indonesia. The data used in this studywere collected mainly through an interview survey usingstructured questioner to 103 respondents, including 61respondents as and 42 respondents as non-adopters ofpaddy’s threshing machine. It was conducted in 4 (four)sub-district (Ciruas, Lebak Wangi, Pontang, Tirtayasa) inSerang district area for the first season of 2014/2015cropping season with descriptive statistics and probitlogistic regression model as a tool for data analysis.Empirical study shows that the significant factorsinfluencing the adoption of power threshers positively arefarm size and financial source, and the significant factorsthat influencing the adoption of power thresher negativelyare the labor availability including the number of thehousehold member who working in farm and side job as alabor and threshing cost. This is true in the case of a non-adopter area where high labor availability prevents the useof power thresher because of the possibility of conflict dueto labor displacement. However, dissemination informationabout the machine through agriculture extension activity bystakeholder (government, extension officer, farmers) to thefarmer as a user is the important factor to increase adoptionof agriculture technology, especially this kind of threshingmachine.

Adopter, powered-thresher, rice, yield loss

DP-02The partial budgeting analysis of power thresherutilizationfor rice farmingTian Mulyaqin1,♥, Ishii Keiichi2

1 Banten Assessment Institute for Agricultural Technology of BantenProvince (BPTP). Jl. Ciptayasa Km 01 Ciruas-Serang 42182, Banten. Tel.+62-254-281055, Fax. +62-254282507. ♥email:[email protected] Graduate School of Agricultural Science, Tohoku University, Japan


Adoption of rice paddy threshing machine such as “powerthresher” is still very low, one of them because of the lackof information about the advantage of this machineeconomically. The objective of this study was to comparethe cost and benefits between power thresher adopter andnon-adopter using partial budget analysis. Data werecollected by a survey of 103 farmers, including 61 farmerswho have adopted threshing machines and 42 farmers whoare non-adopters in the first season of rice paddy farm in2014/2015 in Serang District Banten Province, Indonesia.The results indicated that the power thresher adopterprovides higher yield because of low yield loss, less cost,and higher additional net income than non-adopter. Provedby the marginal rate of return is about 5.51 for powerthresher adopter. It is recommended for farmers to continueto use power thresher than manual way.

Adopter, partial budget analysis, powered thresher, ricepaddy

DP-03Society empowerment model, harvesting techniqueand production potential of Trigona spp. in Kahu,Sub District of Bontocani, District of Bone, SouthSulawesiBudiaman♥, Octaviani W, Sadapotto A, IswaraGautama, Andi Mujetahid, Baharuddin, MuhammadAgungForestry Departement, Faculty of Forestry, Hasanuddin University.Jl.Perintis Kemerdekaan km.10, Makassar 90245, South Sulawesi,Indonesia. Tel.: +62-411-585917, Fax.: +62-411-585917, ♥email:[email protected]

Raising of Trigona spp. bees have been done bycommunity in Kahu, sub district of Bontocani, district ofBone, South Sulawesi, Indonesia. This research purposedto know society empowerment model, harvesting techniqueof honey and production potential of Trigona spp. bees peryear in Kahu, sub district of Bontocani, District of Bone.The method that using in this research is survey method bycollecting data primary data from Direct Observation,Questionaire, Interview, and Focus Group Discussion,while secondary data is collected from literature studywhich have correlation with all of the descriptive dataanalyzing . Result of this research showed that harvestingtechnique that found consisted of four techniques i,e.: arebrooming billa’, technique produces 1.3 kg of honey and0.7 kg propolis/stup, knocking stup technique produced 1kg honey and 0.5 kg propolis/stup, empty stup trappingtechnique produced 1.2 kg honey and 1.9 kg propolis/stup,and stup joint technique produced 1.7 kg honey and 0.9 kgpropolis/box. Production potential in this area is 6.411kg/year of honey and 1.388 kg/year of propolis.

Empowerment model, harvesting technique, productionpotential, Trigona

Bioscience

EO-01On skeletochronology of Asian grass frogFejervarya limnocharis (Gravenhorst, 1829) fromJava, IndonesiaNi Luh Putu Rischa Phadmacanty♥, Amir HamidyResearch Centre for Biology, Indonesian Institute of Sciences. CibinongScience Center, Jl. Raya Bogor Km 46 Cibinong, Bogor 16911, WestJava, Indonesia. Tel.: +62-21-876156, Fax. +62-218765062, ♥email:[email protected]

Asian grass frog Fejervarya limnocharis (Gravenhorst,1829) is distributed from Indochina to Indonesia (Java,Sumatra, and Kalimantan). This frog commonly found inopen habitat such rice field and secondary forest. Apartfrom being a common species, they are also being utilizedas feed sources by Arowana fish industries. Highexploitation on this species needs further data on thebiology, including the age of the species when they wereharvested. The aim of the study was to determine the age ofharvested individuals. Here we examined skletochronology(age determination through the number of line in the bone)of F. limnocharis from Java. We used paraffin methods andstained with hematoxylin for 69 samples (male 46, female23). The results showed that harvested male F. limnocharisat reproductive age were ranged from 1 to 3 years, femalewere ranged from 2 to 3 years while juvenile were from 0to 1 year old, respectively. This information is useful todetermine the management of conservation of this speciesin Java.

Age determination, Asian grass frog, Fejervaryalimnocharis, Java scletochronology,

EO-02Mycorrhizal inoculum potent of the peat soils inTumbang Nusa, Central KalimantanHesti Lestari TataForest Research and Development Center, Ministry of Environmental andForestry. Jl. Gunung Batu No. 5. PO Box 165, Bogor 16001, West Java,Indonesia. ♥email: [email protected]

Indonesia covers the largest peatlands in Asia, however,owing to channel development, transformation to moreintensive land use, and fire, many peatlands have beendegraded and deforested. Peat swamp forest rehabilitationhas been started since years ago, using native tree species,such as Dyera pollyphylla a swamp jelutong, which has anassociation with arbuscular mycorrhizal fungi (AMF). Theresearch aimed to assess inoculums potential of AMF inpeat soils that were collected from three land use types, viz.secondary peat swamp forest, recurrent-burnt peat swampforest, and peatland Agroforestry, in Tumbang NusaCentral Kalimantan. AMF potent was observed from peat


soils using wet-sieving methods and root staining. Resultsshowed that land use types affected mycorrhiza inoculumspotential in the soils. Soils of secondary peat swamp foresthas the highest potent of mycorrhizal inoculums, followedby soils of recurrent burnt peat swamp forest and peatlandagroforestry, which were 43.3, 41.3 and 37.3 propagulesper 10 g of soils, respectively. D. polyphylla seedlings thatwere grown in the nursery have been infected naturally bynative AMF with 43±16.4% colonization. It assumes thatpeat soils in the surroundings area contains sufficientpropagule of AMF, which is beneficial for forestrehabilitation effort.

Dyera polyphylla, fire, forest rehabilitation, peatlandagroforestry

EO-03Flowering morphology, flower development andinsect visitors to flowers of Nepenthes mirabilis(Lour.) Druce.Tri HandayaniCenter for Plant Conservation-Bogor Botanic Gardens, IndonesianInstitute of Sciences. Jl. Ir. H. Juanda No. 13, P.O. Box 309, Bogor 16003,West Java, Indonesia. Tel./fax.: +62-251-8322187. ♥email:[email protected]

Nepenthes mirabilis Druce is a commercial ornamentalpitcher plant belonging to the Nepenthaceae family. Thisspecies is often used as a parent plant in artificialcrossbreeding.The plant is also used in traditionalmedicine, rope-making, handicraft and bouquets. Flowerdevelopment and pollen maturity are important factors inpitcher plant crossbreeding. However, information about itsflowering is still lacking. This study aimed to identify theflowering morphology, flower development, and faunalvisitors to flowers of N. mirabilis planted in Bogor BotanicGardens. Twelve racemes of flowers were taken as asample for observing the process of inflorescencedevelopment, while ten flowers on each raceme wereobserved for investigating the flower blooming pattern. Themorphology of flowering, the flowering process, the flowerblooming pattern, the number of blooming flowers, thelongevity of blooming, and the appearance of insect (and/orother faunal) visitors to flowers were observed andrecorded, using naked eyes, a hand lens, and a camera. Sixphases of inflorescence development were identified:growing of shoot phase, growing of inflorescence phase,opening of raceme-protecting leaf phase, inflorescence-stalk and flower-stalk growth phase, blooming flowerphase and pollen maturity phase. Four phases of flowerdevelopment were observed: growing of flower bud,opening of tepals, blooming of the flower, and flowersenescence. The pattern of flower blooming within aninflorescence was from the base toward the tip. Thenumber of flowers per raceme was 56 to 163. The longestduration for the blooming of a flower was 11 days (30.7%of flowers). The length of the raceme-stalks was 17-31 cm.The length of the racemes was 23-38 cm. The most

common visitors to the flowers were stingless bees,Trigona apicalis.

Flower, Nepenthes mirabilis, pollen, pitcher plant, andraceme

EP-01Characterization of cellulase enzyme fromthermophilic bacteria isolates based on ph andoptimum temperatureEnni Ayu Safitri♥, Maswati Baharuddin, SappewaliJurusan Kimia Sains, Fakultas Sains dan Teknologi, UIN AlauddinMakassar. Jl. Sultan AlauddinSamata-Gowa, No.36, ♥email:[email protected]

Cellulase enzyme can be isolated from microorganisms thatare resistant to high pH and temperature. Cellulase enzymehas a different character depending on the source and theenzyme environment. This study aimed to characterize thecellulase enzymes from thermophilic bacteria isolateswhich includes the determination of the optimum pH andtemperature of the enzyme activity. In this study, anenzyme produced from isolates of thermophilic bacteria bycentrifugation cold 4°C with a speed of 3000 rpm for 15min to obtain a crude extract of the enzyme cellulase.Determination of pH performed using phosphate bufferwith pH variation (6,0; 6,5; 7,0; 7,5; and 8,0) and boric acidbuffer of pH (8,6 and 9,0), while for the determination ofdone an optimum temperature variations in temperature(30, 40,50,60 and 70)°C enzyme activity were furthertested using the Nelson-Samogy measured on a UV-Visspektrofotometer at a wavelength of 545 nm. Resultshowed greatest activity at pH 8 enzyme cellulase activityof 8,6347 x 10-4 U/mL while the optimum temperature of40°C with activity obtained at 17,7217 x 10-4 U/mL.

Thermophilic bacteria, cellulase, pH, temperature

EP-02Relationship between Self-directed Learning andAcademic Motivation in Nursing StudentsMasoomeh Adib1, Shokoh Varaei2, ShahrzadGhiyasvandian3,♥, Zahra Atrkar Roushan4

1PhD Student at School of Nursing and Midwifery of Tehran University ofMedical Sciences, Theran , Iran , and Lecture of Shahid Beheshti Schoolof Nursing and Midwifery of Guilan University of Medical Sciences ,Rasht , Iran.2Assistant Professor, Member of Medical-Surgical Group,School ofNursing Midwifery Tehran University of Medical Sciences, Tehran, Iran.3Associate Professor,Member of Medical-Surgical Group,School ofNursing Midwifery Tehran University of Medical Sciences, Tehran, Iran.4Associate Professor, Biostatistic, Medical Collage of Guilan Universityof Medical Science, Rasht, Iran.

Introduction: rapid development of knowledge andinformation in various fields of medical sciences indicates


the need to encourage self-directed learning in nursingstudents in order to provide quality and updated care. Thisstudy examined the relationship between self-directedlearning and academic motivation in nursing students.Materials and Methods: through a descriptive-correlationalstudy, 305 nursing students studying at the Shahid BeheshtiSchool of Nursing and Midwifery, Rasht were recruited bycensus. These students were asked to fill SRSSDL andAMS. Reliability of these scales was confirmed in previousstudies. Data was analyzed by descriptive (mean andstandard deviation) and analytic tests (Pearson coefficientof correlation and Chi square). Results: there is a positiveand significant relationship between self-directed learningand academic motivation (r=0.164; p=0.004). Amongcomponents of self-directed learning, the highestcoefficient of correlation was related to interpersonal skills(r=0.125) and the lowest was related to evaluation(r=0.111). Conclusion: findings showed a significantrelationship between academic motivation and self-directedlearning. Planners and decision-makers need to plan forincreasing academic motivation of students, facilitating anddeveloping self-directed learning to provide optimal carefor patients.

Self-directed learning, academic motivation, lifelonglearning, nursing student

EP-03Presence of arbuscular mycorrhizal fungal sporesassociated with corn planted with traditional andmore modern farming systemsLily Ishaq♥, Anthonius S. J. Adu Tae, Moresi A.Airthur, Peters O. BakoFaculty of Agriculture, Nusa Cendana University. Jl. Adi Sucipto PenfuiKupang, East Nusa Tenggara Timur. ♥email: [email protected]

Mycorrhizal fungi play an important role in improvingplant productivity and soil conservation, therefore could beconsidered as a strategy for sustainable management ofagricultural ecosystem. One factor that may have impact onthe population of mycorrhizal fungi in the soil is landmanagement. The study undertaken at 6 sites in KupangDistrict of East Nusa Tenggara Province Indonesia aimedto investigate the impact of land management on the sporedensity of arbuscular mycorrhiza (AM) associated withcorn. Two farming systems (traditional and more modern)were chosen for the study, and three locations wereselected to represent each farming system practiced.Representative soil samples were taken from eachlocation/farming system for AM observation and soilanalysis. The results showed that spore density of AMfungi was higher in soil taken from corn plantation withtraditional practice than that found in soil taken from cornplantation with more modern practice, indicating a negativeimpact of more modern farming practice on the populationof AM fungi. The research should be extended to furtherinvestigate the diversity and communities of AM fungi atdifferent time and location.

Arbuscular mycorrhizal fungi, farming practice, landmanagement

national seminar on biodiversity · 2019. 2. 21. · time schedule national seminar on biodiversity...

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