nolineTM

Natural wrinkle lipofiller

ANTI-WRINKLES

Mature skin does not have the same vitality or repairing capacity than young skin. When aging, fragility and cell degradation increase, at the same time the matrix and its main components (elastin and collagen) diminish. This process leads to a thickness decrease up to 20%.

Due to intrinsic and extrinsic aging (solar radiation), expression lines (only affecting the epidermis) and wrinkles (affecting also the dermis) appear.

Adipose tissue plays an important role as it has pre-adipocytes, the precursor cells of adipocytes, the latter being able to store lipids. The maturing process (adipogenesis) increases tissue volume and its surface appears smoother and more homogeneous.

Mature skin has the same amount of pre-adipocytes than young skin but its maturing capacity diminishes. Some areas get hypertrophied (inferior face area, chin) and some others become atrophied (forehead, mouth, periorbital and perioral area).

The global result is a dramatic change in the face morphology with irregular filled areas and with wrinkles and expression lines happening to be more evident.

Natural wrinkle lipofiller

nolineTM

nolineTM stimulates the creation of new adipose cells and fill the wrinkles and expression

lines typical of mature skin

A younger skin without surgery

The reasons behind wrinkles

nolineTM replenishes without surgerynolineTM is composed by a polyphenol from nutmeg seeds (Myristica fragans), macelignan.

It acts on the cell differentiation process of the adipose tissue (in the pre-adipocyte / adipocyte step).

Through a receptor of skin fatty cells, it activates and increases adipogenesis. It also enlarges vacuoles volumes, where lipids are stored.

DERMIS

ADIPOCYTESPRE-ADIPOCYTES

DERMIS

ADIPOGENESIS

BEFORE AFTER

The safest and most natural way of replenishing and improving the appearance of wrinkles and expression lines is acting at a topical level with active ingredients, like nolineTM, designed to

stimulate the natural production of adipose tissue

Macelignan

results• Main wrinkle

- Maximum reduction: 19%- Average decrease: 3.4%

• Average roughness- Maximum reduction: 25%- Average decrease: 2.4%

• 76% of volunteers had a positive response

In vitro efficacy In vivo efficacy

CO

NTR

OL

CU

LTU

RE

nolin

eTM C

ULT

UR

EANTI-WRINKLES

Analysis of the lipid storage, as a sign of the cell maturing process: more lipid vacuoles indicate more volume.

Analysis of the aP2 gene expression

Emblematic of mature adipocyte (lipid storage). Its induction is calculated when adding nolineTM at two different concentrations.

700

600

500

400

300

200

100

0

% G

ENE

IND

UC

TIO

N

CONTROL nolineTM

(3 ppm)nolineTM

(8 ppm)

Up to 600%

Lipid storage

+22%

25 volunteers, between 35 and 50 years old. Experimental area: eyes contour. Application of an active formula (0.25% nolineTM

) on one side and placebo on the other. Two daily applications during 28 days. Measurements at D0 and D28, FOITS (3D image) and efficacy.

D0 D28

Wrinkle decrease

WRINKLE DECREASE

DEP

TH IM

PR

OVE

MEN

T (%

)

5

4

3

2

1

0MAIN WRINKLE AVERAGE ROUGHNESS

PLACEBOnolineTM

+3.4%

+2.4%

Results:Main wrinkle:

Maximum reduction: 19%.Average decrease: 3.4%.

Average roughness: Maximum reduction: 25%.Average decrease: 2.4%.

76% of volunteers had a positive response.

nolineTM

Natural wrinkle lipofiller

ANTI-WRINKLES

Technical specificationsnolineTM 72180PROPERTIES

ACTIVE MOLECULES

APPEARANCE

SOLUBILITY

RECOMMENDED DOSE

Macelignan (Myristica fragans)

SolidWhite-pale yellow color

Soluble in oilInsoluble in aqueous solutions

0.1 - 0.25%

FormulationWrinkle filler treatment

% (w/w)

2.002.500.504.003.255.00

80.670.150.300.300.20

0.10

0.50

0.50

0.03

INCI / PCPC

Glyceryl Stearate CitrateGlyceryl Stearate, Ceteareth-20nolineTM

Diethylhexyl CarbonatePolysilicone-15Hydrogenated Polyisobutene

A

Aqua (Water)Disodium EDTAPotassium SorbateSodium BenzoateSodium Polyacrylate

B

C

Polyacrylate-13, Polyisobutene, Polysorbate 20Acrylamide/Ammonium Acrylate Copolymer,Polyisobutene, Polysorbate 20

D

Aqua (Water), Lactic AcidE

Parfum (Fragrance)

cosmetic applications• Anti-aging products• Anti-aging specific serums• Treatment of mature skins with wrinkles and expression lines• Eyes and lips contours• Anti-aging hand-treatments• Complement for facial firming products

V01-2013

Enlarges the volume of the subcutaneous adipose tissue, filling wrinkles and expression lines of mature skinIncreases the creation of new adipose cells (adipogenesis) and the size of lipid vacuoles in the adipose tissue