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 BIOCHEMICAL REACTIO

FOR THEIDENTIFICATION OFBACTERIA

  (NITRATEREDUCTION )

PRESENTED BY:MODERATED BY

Department of Mic

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INTRODUCTION

 Biochemical tests are used for the iden

of the organisms

Bacteria produces metabolic products by

various substrates with the help of enzymes .

 The detection of the either these enzymes o

products are utilized in the principle of bio

tests.

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NITRATE REDUCTIONTEST

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MEDIA AND RAEGENT

  Pot!!iu" nitrt#$%NO&(nitrit#'r##)*

  P#+ton#,*

  Di!ti--# /t#r-itr#

 

REAGENT 0 A

Su-+hni-i1 1i2*

A1#ti1 1i(,N)$&34-it

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PRINCIPLE

  nitrate reductase

   NO3 + 2e +2H NO2 + H2O

 NO2 + Sulfanilic acid diazonium salt

 

 diazonium salt + alpha-naphthylamine Red color com

 

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IF NO COLOR

TRUE NEGATI6E

FALSE NE

( NO&(NH&$N8$NO

h5ro95-

NO& to

Zinc Dus

R# 1o

Tru# N#* r#1t

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PROCEDURE

Ino1u-t# nitrt# ;roth(,"-) /ith

or*ni!" n in1u;t# t &hour!

A "- #1h o r#*#nt A n B to;roth

O;!#r# or t-#!t & "inut#! or r#1o-or to ##-o+

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RESULTS

Positive ! "ed colour

#egative! #o colour

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  NITRATE POSITI6E ORGANISMS ?ITHE@CEPTIONS

Ent#ro;1t#ri1# #91#+t0 Y#ri!ini

#nt#ro1o-iti1 ;ior6i;rion1# #91#+t0 6i;rio "#-!1hnio

 P!#uo"on1# #91#+t0 P!#uo"on!i"inut

P!#u. +u1i"o;i-i!

A--i*in1# #91#+t0A--i*#n! #1-i!

P!t#ur#-- 1ni!

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  NITRATE NEGATI6E ORGANISMS ?ITHE@CEPTIONS

$ctinomycetaceae% e&cept! $ctinomyces

$rchnia propionica'isteria monocytogenes

Bordetella avium

 Bordetella pertussis

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