neoplasia - department of pathology, bsmmupathbsmmu.weebly.com/.../5/9/7/0/5970547/neoplasia... ·...
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CLINICAL NEOPLASIAMay 2011
Prof. Mohammed Kamal
Dept. of Pathology, BSMMU
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BASIC CONCEPTS
• WHAT IS NEOPLASIA? Definition
• Non neoplastic growth disturbances
• How tumours are formed And grow? (Biology of
tumour growth)
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WHAT IS NEOPLASIA?
Definition (Sir Rupert Willis)
A neoplasm is
• an abnormal mass of tissue,
• the growth of which exceeds and is uncoordinated with that of the normal tissue
• and persists in the same excessive manner after cessation of the stimuli which evoked the change
• The abnormal mass in purpose less, preys
on the host; is virtually autonomous.
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Non neoplastic growth disturbances
or
Tumour like lesions
• Hamartomas : Lung, nevus, angiomas
• Choristoma (heterotopia, ectopic rest) :
Ectopic thyroid tissue
• Repair process : Keloid
• Hypertrophy / atrophy
• Hyperplasia (macroscopic/microscopic)
• Metaplasia (microscopic)
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Pulmonary hamartoma
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Choristoma
(heterotopic
pancreas)
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Keloid
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Prostatic hyperplasia
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Squamous metaplasia of cervix
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Biology of tumour growth
• Phases in the natural history of malignant tumours
• Loss of normal growth control
• Genetic damage in Cancer
• Multi-step Nature of Cancer
• Environmental Carcinogens
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Biology of tumour growth
• Four phases in the natural history of malignant tumours
• 1. Transformation or malignant change in the target cell –(initiation: DNA alteration or cell change)
• 2. Growth of the transformed cells (tumor-promotion: from single mutated cell to formation of tumor)
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• 3. Local invasion (tumor-progression:development of malignancy)
• 4. Distant metastasis
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Heredity and neoplasia
• Inherited cancer syndromes
– Retinoblastoma
– Familial adenomatous polyposis
– Multiple endocrine neoplasia syndrome (MENS)
– Ca breast, ovary, colon and pancreatic cancer (Familial cancer )
– Xeroderma pigmentosum
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Genetic Cancers• Breast and ovarian
• Colon cancers
• Cowden syndrome
• Gastric cancer
• Gorlin Syndrome
• Li-Fraumeni
• Multiple endocrine neoplasia (MEN)
• Neurofibromatosis
• Peutz-Jeghers syndrome
• Phaeochromocytoma
• Retinoblastoma
• von Hippel-Lindau disease
• Wilm’s tumour
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Hereditary Cancer Several affected family
members
Earlier than average age of onset
Multiple generations are affected on one side of the family
A particular pattern of cancers noted
Individuals with more than one primary tumour site
5-10% of Cancer Cases
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Hereditary Breast and Ovarian Cancer
• Most cases caused by a mutation in
BRCA1 or BRCA2 gene
• BRCA1 / 2 are tumour suppressor genes,
which are involved in the repair of DNA
• Accounts for about 5% of breast cancer
cases and about 12% of ovarian cancer
cases
BRCA1BRCA2
5-10%
Other genes
Sporadic
Hereditary
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BRCA1 -Associated Cancers:
Lifetime Risk
Increased risk of other cancers, eg about double
population risk for prostate cancer in men
Breast cancer 56%-87%
(often early age at onset)
Second primary breast cancer 64%
Ovarian cancer 16%-44%
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BRCA2-Associated Cancers:
Lifetime Risk
Other cancers:
pancreatic
malignant melanoma
breast cancer
(50%-80%)
ovarian cancer
(15-27%)
male breast cancer
(7%)contralateral breast
(50%)
prostate
(~30%)
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• Nonhereditary predisposing conditions• Regenerative, hyperplastic and dysplastic proliferations
• • Bronchial metaplasia and dysplasia
• • Cirrhosis of liver
• Oral ulcer due to sharp tooth
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• TAXONOMY & NOMENCLATURE
• Classification of tumours
• Characteristics of benign and malignant neoplasm
• Spread of malignant tumours
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TAXONOMY & NOMENCLATURE
• ON BIOLOGICAL BEHAVIOUR DIVIDED INTO:
• Benign tumour. oma suffix to cell of origin
–osteoma, fibroma, adenoma (gland or no gland formation),
papilloma, cystadenoma, papillary cystadenoma.
• Malignant tumour : Sar- prefix (fleshy) sarcoma, carcinoma,
–e.g. adenocarcinoma.
• Confusing names: Melanoma, lymphoma, seminoma.
• Special names: Hodgkin’s disease, Wilm’s tumour
(nephroblastoma) more than one name.
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Tumours have two components–Parenchyma (Proliferating cells) and
–Stroma (Connective tissue, blood vessels & lymphatics)
• Parenchyma determines biologic behavior
• Stroma is supporting framework
Stroma
Parenchyma
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Name based on naked eye morphology :
Papilloma: Finger like structures
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Polyp: Larger surface projections
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Dentigerous Cyst
Cyst: Space containing fluid
lined by epithelium
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Fungating colonic carcinoma
Ulcerative colonic carcinoma
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Classification of tumours
• Histogenesis:– Tissue of origin e.g. squamous epithelium, mesenchymal tissue, glia etc.
– Practically done by microscopic/ immunologic/ genetic assessment of
tumour
• Behavioral classification:
• based on known or anticipated biologic behavior.
–Two groups: Benign & malignant
–Rarely borderline/unknown
Currently used classification : Combination of the
histogenetic and behavioural features i.e. squamous
papilloma, squamous cell carcinoma etc.
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Characteristics of benign and malignant
neoplasm
–GROSS PATHOLOGICAL FEATURES
• Benign : Capsulated or well circumscribed
• Freely movable
• Firm, uniform
• Retrograde changes less frequent.
• Malignant : Irregular shape, poorly circumscribed
• Projection of growth into surrounding tissue.
• Fixed
• Fleshy (Sarcoma), very firm (Carcinoma).
• Regressive changes (necrosis, haemorrhage)
frequent.
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Gross pathological features
of benign & malignant
tumours
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Malignant tumour
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Characteristics of benign and malignant
neoplasm
• MICROSCOPIC FEATURES:
• Differentiation and anaplasia :
• Differentiation - extent to which parenchymal
cells resemble comparable normal cells both
morphologically and functionally
• Well differentiated- normal appearing cells.
Poorly/Undifferentiated -primitive appearing
• Benign Tu. are well differentiated
• Malignant Tu. well diff. to undifferentiated
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Differentiation
and anaplasia
Well differentiated
poorly differentiated
Tumour cells in groupKeratin pearl
Normal squamous epithelium
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Adenocarcinoma
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Characteristics of benign and malignant
neoplasm
• Cellular changes (anaplasia)
• Pleomorphism Large or very small cells.
–Nuclei hyperchromatic
– disproportionately large 1:1= N:C
– Shape variable.
– Chromatin coarsely clumped
– Large nucleoli
–Cytoplasm No typical change
–Mitoses increased and may be atypical
–Tumour giant cells
• Orientation of cells markedly disturbed.
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Anaplasia:
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Characteristics of benign and malignant
neoplasm
–Dysplasia pleomorphism / dark nuclei/ mitoses are normal but
in abnormal locations / Architectural anarchy.
–Loss of uniformity of individual cells as well as loss of their
architectural orientation
–Carcinoma in situ: marked dysplasia involving whole
thickness of the epithelium. Dysplasia does not necessary
progress to Carcinoma. May revert if inciting came is removed.
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Dysplasia &
carcinoma in situ
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Characteristics of benign and malignant
neoplasm
–Functional differentiation
–Benign & well differentiated malignant tumours elaborate
normal function (secretion, keratin etc) Other tumours may not
be functional. Yet other may produce ectopic hormones
–Rate of growth
–Benign slow growth, depends on blood supply, other factors
–Malignant rapid, erratic pace. In general growth rate
correlates with level of differentiation.
–Exceptions - Leiomyma show rapid growth in pregnancy.
Malignant tumours may show slow growth for years of them
suddenly in increase size. CLL, low grade NHC, where an
aggressive subclone emerges.
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Characteristics of benign and malignant
neoplasm
• Local invasion
• Benign tumours –do not invade, infiltrate or
metastasize
• Malignant tumour – infiltrate, invade and destroy
surrounding tissue
• Metastasis
• Metastases are tumour implants discontinuous with
the primary tumour.
• Benign tumours do not metastasize
• Malignant tumours frequently metastasize.
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Spread of malignant tumours
• Local spread
–surrounding tissue
–lymphatic vessel
–Perineural space
–venous / arterial invasion
–serous spaces
–Local invasion is an important sign necessary
for the diagnosis of malignant tumour at the
primary site
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Local invasion of malignant
tumours
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Spread of malignant tumours
• Metastasis or distant spread
–Lymphatic spread
–Blood spread
–Seeding of body cavities or surfaces]
–Transplantation
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Metastasis
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Lymphatic spread
Most common pathway to spread for carcinoma
Sarcoma may also use this route
LN involvement follows natural routes of drainage
Skip metastasis or retrograde involvement may occur.
LN enlargement near a cancer is not always due to metastasis
may be due to Reactive changes
Extent of LN involvement is important in assessing prognosis / Staging of tumour
Spread of malignant tumours
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Hematogenous spread
Typical of sarcoma but carcinoma may also use this pathway
e.g. renal cell carcinoma, HCC
Venous invasion common
Arteries are rarely involved
Metastatic deposits are found in liver, lung, brain, bone etc.
Responsible for most of the cancer deaths
Limits surgical and radiotherapeutic treatment
Spread of malignant tumours
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• Nonhereditary predisposing conditions
• Precancerous conditions
• • Chronic atrophic gastritis of pernicious anaemia
• • Solar keratosis
• • Chronic ulcerative colitis
• • Leukoplakia
• • Villous adenoma of colon
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Clinical aspects of neoplasia
• Grading and staging of tumours
• Tumour immunity
• Effects of tumour on host
• Laboratory diagnosis of tumour
• Examples of common human tumours
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Tumour Grade• Tumor grade is system used to classify
cancer in terms of how abnormal they look
under microscope
• Factors considered for tumor grading:
– The differentiation of the tumour cells and
– Growth pattern of the cells.
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GRADING SYSTEMS
Grading Systems
- well, moderately, or poorly differentiated
- Grade 1, 2, 3
- Grade I, II, III, IV
OTHER SYSTEMS
The higher the grade, the worse the
prognosis
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NEOPLASIA, Broder’s
Grading
• Grade 1--->75% cells differentiated
• Grade 2--->50% cells differentiated
• Grade 3--->25% cells differentiated
• Grade 4---<25% cells differentiated
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Grading of Squamous Cell
Carcinoma• WELL MODERATE POOR
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Does the same grading scale apply
to all tumors?
NO!
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TUMOUR STAGING
• Staging describes :
• the extent or severity of an individual’s cancer
• based on
– the extent of the original (primary) tumour and
– the extent of spread in the body.
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Purposes
• Aids in planning treatment
• Gives some indication of prognosis
• Assists in evaluating the results of
treatment
• Facilitates the exchange of information
between treatment centers
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What is the TNM system?
• The TNM system is one of the most commonly used staging systems.
• This system has been accepted by the International Union Against Cancer (UICC) and the American Joint Committee on Cancer (AJCC).
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• The TNM system is based on:
– The extent of the tumor (T),
– The extent of spread to the lymph
nodes (N), and
– The absence or presence of
metastasis (M).
• A number is added to each letter to
indicate the size or extent of the tumor
and the extent of spread
• e.g. T2 N1 M0
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Numerical Subsets of TNM
Definitions of T
Extension of Primary Tumor
• TX Primary tumor cannot be assessed
• T0 No evidence of primary tumor
• Tis Carcinoma in situ
• T1-4 Increasing size and/or local extent of the primary tumor
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Numerical Subsets of TNM
Definitions of N
Involvement of Regional Nodes
• NX Regional lymph nodes cannot be
assessed
• N0 No regional lymph node metastasis
• N1-3 Increasing involvement of regional
lymph nodes
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Numerical Subsets of TNM
Definitions of M
Distant Metastasis
• MX Distant metastasis cannot be
assessed
• M0 No distant metastasis
• M1 Distant metastasis
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Stage Grouping
• Group TNM into a stage
• Assignment is based on values of T,N,M
• Each site has a different stage grouping
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Tumor
Immunology/Immunotherapy
• How does our immune system eliminate
cancer cells?
• How do cancer cells escape from
Immunosurveilance?
• How can we manipulate immune response to
kill cancer cells?
• Current approaches of tumor immunotherapy.
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Evidence for Tumor Immunity
• Spontaneous regression: melanoma, lymphoma
• Regression of metastases after removal of primary tumor: pulmonary metastases from renal carcinoma
• Infiltration of tumors by lymphocytes and macrophages: melanoma and breast cancer
• Lymphocyte proliferation in draining lymph nodes
• Higher incidence of cancer after immunosuppression, immunodeficiency (AIDS, neonates), aging, etc.
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TUMOUR ANTIGENS
Classification of tumour antigen .
Previously (1) Tumour specific antigen.
(2) Tumour associated antigen.
TSAs evoke a cytotoxic T cell
TAAs do not evoke an immune response
Detection of these TAAs is of value in the diagnosis of
certain tumors, and antibodies raised against them
can be useful for immunotherapy.
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Tumour Markers
• Types of Tumour markers
– Cell surface antigens.
– Cytoplasmic proteins.
– Enzymes.
– Hormone.
• Criteria of an Ideal Tumour Marker
– Specific
– Sensitive
– The method of assay must be cheap & easy
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LABORATORY DIAGNOSIS
OF TUMOUR
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DIAGNOSIS OF TUMOURSThe diagnosis of patients with neoplastic disease requires a logical and
careful application of
• history taking
• clinical examination and
• appropriate special tests and investigations.
• Many tests can be used including radiological imaging, haematological assays and biochemical tests but
• ULTIMATE diagnosis of neoplasia requires histopathological assessment or a tissue diagnosis.
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Tissue Diagnosis of neoplasia
1. Histologic methodsRoutine
Quick frozen section
2. Cytologic MethodExfoliative
Aspiration
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Types of biopsy procedures
• main types of biopsy procedures.
• Incisional biopsy
• Excisional biopsy.
• Endoscopic biopsy
• Core needle biopsy
• Shave biopsy.
• Punch biopsy.
• Bone marrow biopsy
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Incisional &
Punch
biopsy
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Excisional biopsy
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AFTER BIOPSY SURGICAL
SPECIMENS
In requisition give patient’s name &
identification
• Adequate clinical details
• Correct labeling of specimen bottle
• Adequate preservation of sample (10%
formalin; 1:10 proportion)
• Biopsy fixative - purposes of fixation : inhibit autolysis and bacterial overgrowth
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Techniques in Pathology:
• Gross Pathology:
• Light Microscopy:
– Histopathology, Cytology
• Histochemistry, Biochemical
• Immunohistochemistry
• Electron Microscopy
• Cell Cultures, Medical Microbiology
• Molecular Pathology
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GROSS EXAMINATION
• Description
• Specimen weight and measurement
• Consistency
• Photography
• Representative sections
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GROSS EXAMINATION- 2
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Tissue Processing :
TISSUE PROCESSOR
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Block and slide
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Sectioning
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staining
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Microscopic examination
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Frozen section
Freezing fresh tissue ( -20 to -30 C ) on cryostat stage, sectioned, stained and examined under microscope.
Bypasses routine processing The whole process takes average 20 to 30 minutesTherefore, report can be obtained while patient is on operating
table
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Indication for frozen diagnosis
1. To make an immediate therapeutic decision extent of the procedure must be made BENIGN VS MALIGNANT
2. To determine the adequacy of surgical margin3. To evaluate adequacy of biopsy material
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Frozen section
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Surgical Pathology Report
• Gross description of the specimens : • Microscopic description• Diagnosis
Organ, specific site in that organ, operation :-The morphologic diagnosis
Example: Dx: Cheek, buccal , right side, biopsy :
Invasive squamous cell carcinoma, grade II• Note or Comment
Differential diagnosis, give the reason for this diagnosis, Selected reference
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Limition of histologic diagnosis
• Inadequate tissue i.e. tiny sample
• Unsatisfactory for interpretation- edge of a ulcerating squamous carcinoma– pseudoepitheliomatous hyperplasia
– inadequate in depth
– Inproper fixation/ autolysis• Incomplete or no history
• Crush artifact
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Cytopathology:
• Cytopathology is study of cells in diagnosis of disease.
• Exfoliative & Non-Exfoliative - cytology.
• Exfoliative: Cell samples are collected from normally shedding tissues like epithelium. spatula or brush to enhances collection.
• Non-Exfoliative: Cells samples collected by needles with suction pressure. (FNAC)
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CYTOPATHOLOGY VS.
HISTOLOGY
• Rapid, non-invasive, cheap, fewer
complications
• Lower sensitivity, specificity, requires
expertise in interpretation
• Histology is the gold standard
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Cytology of oral carcinoma
Normal smear
large, flattened squamous cells
no malignant cells
Abnormal smear
pleomorphic, Hyper-chromatic nuclei
Malignant cells
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FNAC – Fine Needle
Aspiration Cytology
• Aspiration of cells/ tissue fragments using fine
needles ( 22 , 23, 25 G) ; external diameter 0.6
to 1.0 mm
• Diagnostic materials in the needle and not in
the syringe even in cystic lesions
• Smears made on slides
• Stained
• Examined under microscope
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FNAC
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Cytology vs Histology
Papillary carcinoma of thyroid - follicular variant
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Fluid cytology
• Peritoneal pleural, pericardial cytology.CSF,urine, cyst fluid etc.
• They have a different morphology because they are in a cozy environment.
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Malignant cells in effusion fluid
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Immunohistochemistry
Categorization of undifferentiated neoplasm
Specific antibodies (monoclonal) are used to
identify cell products
and surface markers, e.g. Keratins– carcinoma
Categorization of leukemia's and lymphomas.
Identification and classification of tumours
arising from T and B lymphocytes
Determination of site of origin of metastatic
tumours
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Immunohistochemistry
• Antigen antibody
reaction
• Ab Tagged with
marker
– Simple Dye
– Enzyme
(peroxidase)
– Fluorescent Dye
– Radioactive Dye
Marker
Sec. Antibody
Pri. Antibody
Tissue Antigen
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NPC
H & E Pan CK
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MOLECULAR DIAGNOSIS
• PCR– T-cell R, IG gene
• Monoclonal = neoplastic, polyclonal = reactive
– BCR-ABL transcripts• CML minimal residual disease
• FISH– Translocations
• Ewing’s sarcoma, leukemias, lymphomas
– Amplification• HER-2 = breast, N-MYC = neuroblastoma
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MOLECULAR PROFILING
• Tumor Profiling means to obtain and process complex information from tumors or their precursors that can be used to optimize classification for the purpose of
• Diagnosis
• Staging
• Prognosis prediction
• Therapy selectionHereditary predisposition to cancer
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MOLECULAR PROFILING involves
• DNA microarray analysis
• Gene chip technology
• Simultaneous measurement of expression levels of thousands of genes
• mRNA - cDNA - fluorescent - hybridize with DNA probes on solid support (silicone chip) -laser
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Flow cytometryUseful for measuring:Membrane antigens for leukemia's,
lymphomas DNA content of tumour cells
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Tumour markersBiochemical indicators of the presence of a tumour.Include --- cell surface antigens, cytoplasm
proteins, enzymehormones.
Carcinoembryonic antigen (CEA)
Positive in:60 to 90% colorectal Ca50 to 80% pancreatic Ca25 to 50% gastric and breast CaAlpha- fetoprotein (AFP)Abnormal levels in :Ca of liver and germ cells of testis
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Clinical features of tumoursAll tumours have potential for morbidity and mortality
All masses require anatomic evaluation (Histodiagnosis)
Effects of tumour on host1. Local and hormonal effects
• Location – pituitary adenoma- endocrinopathy-Gut-
obstruction
• Tumours of endocrine glands may produce hormones
-cell adenoma hypoglycemia
• Erosive growth, ulceration, bleeding (melena,haematuria),
secondary infection
• Torsion of tumour in mobile organ infarction
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Cancer cachexia
Loss of body fat and lean body mass with profound
weakness, anorexia and anemia
Probably due to TNF-, IL-1, INF- etc.
Proteolysis inducing factor (PIF)
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Paraneoplastic Syndrome
• A constellation of symptoms and sings
at a site distant to the primary tumor,
unrelated to local effects or metastases.
•
• Syndrome complexes
• Can not readily be explained
• 10% cases of advanced cancer
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Causes of Paraneoplastic Syndrome
(PNS)
• Mechanism is not fully understood.
• Ectopic production of peptide proteins (hormones): SIADH, Cushing’s syndrome
• Immunologic mechanisms: anti-Hu antibiody syndrome
• Release of cytokines: anorexia-cachexia
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Importance:
• Earliest manifestatin of an occult neoplasm
• Significant clinical problems
• Mimic metastatic diseases
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Examples of paraneoplastic syndrome
• Cushing’s syndrome- small cell Ca lung- ACTH/ pro-opiome-lanocrtin (POMC)
• Hypocalcaemia : 1. S.C.C. of lung
• 2. Br Ca, renal Ca, ovary-parathyroid hormone
related protein PTHrP
• Hypoglycaemia– fibrocercoma--- insulin
• H.C.C, other sarcoma
• Acanthosis nigricans-- gastric carcinoma? EGF? Immunologic, lung carcinoma
• Clubbing of fingers & hypertrophic osteoarthropathy- - Bronch. Ca, unknown
• Venous thrombosis– pancreatic Ca.
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Features of a benign tumour are:
• Always encapsulated
• Slowly growing
• Have frequent mitosis
• May be poorly diffrentiated
• Do not metastasize
• F T F F T
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Regarding grading of malignant
tumours:
• It depends on cellular differentiation
• It determines staging
• Single grading system is used for all tumours
• Lymph node examination is needed
• It is done under microscope
• T F F F T
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Regarding spread of a tumour:
• Carcinomas principally spread by lymphatics
• Sarcomas do not metastasize to brain
• Metastasis indicates poor prognosis
• Metastasis can be evaluated by FNAC
• All malignant tumours have same petstatic
potential
• T F T T F