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Neuroanatomical techniques Golgi - whole neuron Nissl - (cresyl violet) - cell bodies Electron microscope - everything Myelin Stain - Tracing techniques - anterograde - retrograde. Trace Connections retrograde (afferent) anterograde (efferent) - immunochemical - PowerPoint PPT Presentation

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Page 1: Neuroanatomical techniques Golgi - whole neuron Nissl - (cresyl violet) - cell bodies
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Neuroanatomical techniques

Golgi - whole neuronNissl - (cresyl violet) - cell bodies

Electron microscope - everything

Myelin Stain -

Tracing techniques - anterograde- retrograde

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Trace Connectionsretrograde (afferent)anterograde (efferent)

- immunochemical- kidney bean PHA-L

- anterograde- horseradish peroxidase (HRP)

retrograde

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PHA-L injection site in VMH

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PHA-L anterograde VMH to PAG

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Fluorogold - retrograde - VMH - amygdala

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PAG - Anterograde tracing - PHA-LAntibody stain for hormone receptor

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Major Division Ventricle Subdivision Structures

Forebrain Lateral Telencephalon Cerebral CortexBasal GangliaLimbic System

Third Diencephalon ThalamusHypothalamus

Midbrain Cerebral Aqueduct Mesencephalon TectumTegmentum

Hindbrain Fourth Metencephalon CerebellumPons

Myelencephalon Medulla Oblongota

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