Neutrophils protein synthesis dysfunction in community

acquired pneumonia

Mishlanov V.Ju., Perm, 2013

Actuality

• Neutrophils synthesize some antimicrobic peptides, such as defensins, cathelicidines et all.

• Defensins are small Cysteine-rich cationic proteins

Methods

Scheme of leukocytes protein-lipid complexes

release activity

Cholesterol

3-LAProtein

Neutrophil

Method of lipid release leukocytes ability (LRLA)

measurement

100 mcl

400 mcl

50 mcl

Any devices for cholesterol or defensins concentration

determination

Leukocytes lipid release ability in healthy people of different ages

Some proteins and peptides synthesized by Neutrophils

Proteins and peptides Mean value (ng/ml)

Defensins-alfa 115,9±83,81

Lp(a) 35,7±11,75

Pro-BNP 18,3±32,66

Factor VII 2,8±4,70

CRP 1,9±2,08

Amyloid beta ab42 0,8±0,87

Factor von Willebrand 0,032±0,016

Defensins synthesis in elderly patients

Defensins (ng/ml)

115,9

62,1

0

20

40

60

80

100

120

140

Здоровые ИБС

Defensins(ng/ml)

The aim of the study

• Investigate neutrophils protein synthesis in community acquired pneumonia patients

Material of the Study

• 52 CAP patients were examined,

• 25 – male, 27 - female;

• age 20-60 yrs., mean age 40,3;

• 35 – with non-severe and

• 17 – with severe pneumonia

Clinical characteristics

Symptoms No severe Severe

Breath rate 22,6±1,6822,6±1,68 27,0±3,38*27,0±3,38*

Heart rate 85,6±11,5185,6±11,51 94,6±7,23*94,6±7,23*

BP systol. 115,9±9,03115,9±9,03 113,1±7.99113,1±7.99

BP dyastol. 72,6±1,2072,6±1,20 72,5±8,8672,5±8,86

Temperature 38,6±0,6138,6±0,61 38,9±0,6738,9±0,67

Roentgen: Number of segments

2,61±1,202,61±1,20 4,9±2,30*4,9±2,30*

SpO2 96,2±±1,231,23 90,2±2,64*

Age depending LRLA in CAP patients and healthy people

LRLA in nonsmokers CAP patients at the age of 21-40 yrs in accordance with the

severity of the disease

LRLA dynamics according to CAP phase

LRLA in smokers and nonsmokers with CAP

LRLA in CAP and LTB patients (mmol/l)

Conclusion

• The results of the study showed significant antimicrobial peptides neutrophils synthesis dysfunction in community acquired pneumonia patients

• This dysfunction usually was revealed in patients aged after 40 yrs., with severe course of CAP and with lung tuberculosis

Hypotesis

• Antimicrobial peptides synthesis neutrophils dysfunction is one of the basic mechanisms of acute microbial respiratory pathology

• This mechanism is released only in organ tissue, but not in bloodstream, and it may be revealed by cultural diagnostic technique