2

09:30 Bus departs Corinthia Hotel

10:00 – 10:15 Arrival and Registration CCP 249

10:15 – 10:45 Welcome and Introduction of team members CCP 249 Radislav Sedlacek (IMG/CCP)

10:45 – 12:30 Introduction to the course and teaching material CCP 249 Participants: presentation individual projects Björn Schuster (IMG/CCP) and all instructors 12:30 – 13:30 LUNCH

13:30 – 14:00 Lecture: Pros-and-cons of programmable nucleases CCP 249 Radislav Sedlacek (IMG/CCP)

14:00 – 14:30 Lecture: Gene targeting with programmable nucleases: The basics CCP 249 Björn Schuster (IMG/CCP)

14:30 – 16:00 Practical training session: gRNA validation I CCP 115 Jana Kopkanová & Björn Schuster (IMG/CCP) 16:00 – 16:15 TEA & COFFEE

16:15 –17:00 Lecture: Historical perspective - how we came to nucleases SO.001* Lluis Montoliu (CNB, Spain) 17:00 –18:00 Get together with time for discussions with the speaker and instructors CCP 249

18:10 Bus leaves for Corinthia Hotel

19:30 COURSE DINNER I U Tunelů, Karlín (see map 1)

Day 1 – Monday 3rd April 2017

Czech Centre for Phenogenomics

2nd Programmable Nucleases (CRISPR/Cas9)

Transgenesis Course

Sponsors:

3

07:15 Bus departs Corinthia Hotel

08:00 – 08:45 Lecture: Breaking-Cas, a new guide designing program and CRISPR/Cas function SO.001* Lluis Montoliu (CNB, Spain)

08:45 – 09:00 Daily agenda and outcomes overview CCP 249 Björn Schuster (IMG/CCP) 09:00 - 10:00 Practical training session: gRNA preparation I CCP 115 Jana Kopkanová & Björn Schuster (IMG/CCP)

10:00 – 10:15 TEA AND COFFEE

10:15 – 13:15 Practical training sessions:

Group A Zygote injection – Ronald Naumann (MPI-CBG, Dresden) and Inken Beck CCP 265 (IMG/CCP) Group B Genotyping, digital PCR - Jana Kopkanova (IMG/CCP) and Guillaume Pavlovic CCP 115 (PHENOMIN-ICS, Strasbourg) Group C Bioinformatics, gene targeting and CRISPR design - Björn Schuster (IMG/CCP), CCP 249 Marie- Christine Birling (PHENOMIN-ICS, Strasbourg), Lluis Montoliu (CNB, Spain)

13:15 – 14:15 LUNCH

14:15 – 14:45 Lecture: gRNA validation and genotyping of Indel mutations CCP 249 Björn Schuster (IMG/CCP) 14:45 - 16:15 Practical training session: gRNA preparation II CCP 115 Jana Kopkanová & Björn Schuster (IMG/CCP)

16:15 – 16:30 TEA & COFFEE

16:30 – 17:15 Lecture: Tackling complex genome engineering challenges with CRISPR SO.001* Francis Stewart (BIOTEC, TU Dresden)

17:15 –18:00 Get together with time for discussions with the speaker and instructors CCP 249 18:10 Bus leaves for Corinthia Hotel

Free evening

Day 2 – Tuesday 4th April 2017

Czech Centre for Phenogenomics

Sponsors:

4

Day 3 – Wednesday 5th April 2017

07:15 Bus departs Corinthia Hotel

08:00 – 08:45 Lecture: repertoire of gene targeting methods in rodents SO.001* Guillaume Pavlovic and Marie-Christine Birling (PHENOMIN-ICS, Strasbourg)

08:45 – 09:00 Daily agenda and outcomes overview CCP 249 Björn Schuster (IMG/CCP)

09:00 - 10:00 Practical training session: gRNA preparation III CCP 115 Jana Kopkanová & Björn Schuster (IMG/CCP)

10:00 – 10:15 TEA AND COFFEE

10:15 – 13:15 Practical training sessions:

Group B Zygote injection – Ronald Naumann (MPI-CBG, Dresden) and Inken Beck CCP 265 (IMG/CCP)Group C Genotyping, digital PCR - Jana Kopkanova (IMG/CCP) and Guillaume Pavlovic CCP 115 (PHENOMIN-ICS, Strasbourg) Group A Bioinformatics, gene targeting and CRISPR design - Björn Schuster (IMG/CCP), CCP 249 Marie- Christine Birling (PHENOMIN-ICS, Strasbourg), Lluis Montoliu (CNB, Spain), Bernd Zetsche (Broad Institute, USA)

13:15 – 14:15 LUNCH

14:15 –14:45 Lecture: Targeting vector design CCP 249 Björn Schuster (IMG/CCP) 14:45 - 16:15 Practical training session: gRNA validation II CCP 115 Jana Kopkanová & Björn Schuster (IMG/CCP)

16:15 – 16:30 TEA & COFFEE

16:30 – 17:15 Lecture: Expanding the Research Applications for CRISPR SO.001* Guillaume Pavlovic (PHENOMIN-ICS, Strasbourg)

17:15 – 18:00 Get together with time for discussions with the speaker and instructors CCP 249

18:10 Bus leaves for Corinthia Hotel

Free evening

Czech Centre for Phenogenomics

Sponsors:

5

Day 4 – Thursday 6th April 2017

07:15 Bus departs Corinthia Hotel

08:30 – 09:15 Lecture: Development CRISPR-Cas9 & Cpf1 technology SO.001* Bernd Zetsche (Broad Institute, USA) 09:15 – 09:30 Daily agenda and outcomes overview SO.001 Björn Schuster (IMG/CCP)

09:30 - 10:30 Practical training session: gRNA validation III CCP 115 Jana Kopkanová & Björn Schuster (IMG/CCP)

10:30 – 10:45 TEA AND COFFEE

10:45 – 13:45 Practical training sessions:

Group C Zygote injection – Ronald Naumann (MPI-CBG, Dresden) and Inken Beck CCP 265 (IMG/CCP)

Group A Genotyping, digital PCR - Jana Kopkanova (IMG/CCP) and Guillaume Pavlovic CCP 115 (PHENOMIN-ICS, Strasbourg) Group B Bioinformatics, gene targeting and CRISPR design - Björn Schuster (IMG/CCP), CCP 249 Marie- Christine Birling (PHENOMIN-ICS, Strasbourg), Lluis Montoliu (CNB, Spain), Bernd Zetsche (Broad Institute, USA)

13:45 – 14:45 LUNCH

14:45 – 15:15 Lecture: CRISPR/nuclease troubleshooting and genotyping CCP 249 Björn Schuster and Jana Kopkanova (IMG/CCP)

15:15 - 16:45 Practical training session: collection of results and time for discussion/ CCP 115 trouble shooting All instructors

16:45 – 17:00 TEA & COFFEE

17:00 – 17:45 Lecture: High Specificity and Enhanced HDR Efficiency CRISPR/Cas9 for CCP 249 Precision Genome Engineering Qingzhou Ji (MilliporeSigma)

17:45- 18:00 Get together with time for discussions with the speaker and instructors CCP 249

18:10 Bus leaves for Corinthia Hotel

19:30 COURSE DINNER II U Bansethů (PIVOVAR; see map 2)

Czech Centre for Phenogenomics

Sponsors:

6

Day 5 – Friday 7th April 2017

07:15 Bus departs Corinthia Hotel

08:00 – 08:45 Lecture: genome editing using nucleases: new possibilities and application to SO.001* reveal gene function in diseases Marie-Christine Birling (PHENOMIN-ICS, Strasbourg)

08:45 – 09:00 Daily agenda and outcomes overview CCP 249 Björn Schuster (IMG/CCP)

09:00 - 10:00 Collection of results and preparation of short presentations CCP 249 All instructors

10:00 – 10:15 TEA AND COFFEE

10:15 - 12:30 Short group presentations Summary & Conclusions CCP 249

12:30 – 13:15 Lecture: “from genotype to phenotype” SO.001* Guillaume Pavlovic (PHENOMIN-ICS, Strasbourg)

13:15 - 14:15 LUNCH

14:15 End of course

Optional:

14:30 – 16:00 Tour through Czech Centre for Phenogenomics (phenotyping and breeding)

Guest Speakers:Lluis Montoliu (CNB, Spain) Ronald Naumann (MPI-CBG, Dresden)Bernd Zetsche (Broad Institute, USA) Francis Stewart (BIOTEC, Dresden)Qingzhou Ji (Sigma/Merck)

Organising Team: Yann Herault (PHENOMIN-ICS, Strasbourg) Radislav Sedlacek (IMG/CCP)Marie-Christine Birling (PHENOMIN-ICS, Strasbourg) Bjorn Schuster (IMG/CCP)Guillaume Pavlovic (PHENOMIN-ICS, Strasbourg) Inken M Beck (IMG/CCP)

Jana Kopkanova (IMG/CCP)and all other members of Transgenic and Archiving Module

Czech Centre for Phenogenomics

Sponsors:

7

Sponsors:

Czech Centre for Phenogenomics

Bus timetable

The complementary shuttle bus will depart the Corinthia Hotel (Kongresová 1655/1, 140 69 Praha 4) at the stated times. Please gather in the lobby area 10 mins before the scheduled departure. Dr Lluis Montoliu (pictured) will be in the lobby to check all participants are present before the bus departs.

Monday 3rd April: 9.30 amTuesday 4th April: 7.15 amWednesday 5th April: 7.15 amThursday 6th April 7.15 amFriday 7th April: 7.15 am

The shuttle bus will depart the Czech Centre for Phenogenomics (CCP) at 18:10 and will return to the Hotel Corinthia everyday.

Course Venue

The course will take place at the Czech Centre for Phenogenomics, BIOCEV, Prumyslova 595, 252 50 Vestec, Czech Republic.

Lunch

Lunch will be provided as part of the course. Please ensure you have your name badges with you when you go for lunch. This will ensure you are not charged for your meal.

Course Dinners

As part of the course, two course dinners are organised. Please see the attached maps for the locations. More information will be given during the course.

Monday7.30 pm - U Tunelů, Karlín (Map 1)

Thursday7.30 pm - U Bansethů (Map 2)

Certificate of attendance

Certificates will be available at the end of the course.

Dietry Requirements

If you have any special dietry requirements please let us know of them as soon as possible.

Bus time schedule

2nd

programmable nucleases (CRISPR/Cas9) Transgenesis Course Date: 3

rd – 7

th April, 2017

Czech Centre for Phenogenomics, Prumyslova 595, 25250 Vestec

Meeting point and pick-up location: Hotel Corinthia (Corinthia Hotel Prague, Kongresová 1, Prague 4,

140 69, CZECH REPUBLIC) – near Metro station Vysehrad.

Time schedule for the bus (departure times):

Corinthia Hotel – Vestec (morning)

Vestec – Corinthia Hotel (evening)

Monday, 3rd April 2017 9:30 18:10

Tuesday, 4th April 2017 7:15 18:10

Wednesday, 5th April 2017 7:15 18:10

Thursday, 6th April 2017 7:15 18:10

Friday, 7th April 2017 7:15 18:10

8

Maps

Course dinner Monday 7.30 pm - U Tunelů, Karlín

Course dinner Thursday 7.30 pm - U Bansethů

Map 1

Map 2

9

Prague City Metro Map

Change here for bus 326 to Vestec, BIOCEV

Directions from Prague Airport to Hotel Corinthia

From airport take the bus 119 (direction Nádraží Veleslavín) and go to station Nádraží Veleslavín, then go to subway A (direction Skalka) and go to station Muzeum, change the subway to line C (direction Háje) go to station Vysehrad and signs will be there for Hotel Corinthia.

10

Main BIOCEV building

CCP building

Main BIOCEV Campus

CRISPR complete programmeCRISPR completed programmeBus time scheduleCRISPR complete programme