newsletter jan-mar 2013

7/28/2019 Newsletter Jan-Mar 2013

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Setting New Standards

Megazyme International Ireland, Bray Business Park, Bray, Co. Wicklow, IrelandTel: + 353 1 2861220 Fax: + 353 1 2861264 Email: [email protected] Web: www.megazyme.com

We are pleased to announce that we can now offer ultra-pure, recombinant, afnity puried

endo-inulinase (E-ENDOIAN) and exo-inulinase (E-EXOIAN).

These enzymes have been produced at our research facility in Bray, Ireland.They are available separately for research applications and have also been incorporated into theFructan and Fructan HK analytical kits offered by Megazyme.

Megazyme now offers highly pure, recombinant exo-Inulinase fromA. niger.Recombinant

Cat. code: E-EXOIAN

(EC 3.2.1.80) CAZy: GH Family 32

SPECIFIC ACTIVITY:~ 2000 U/mg (60C, pH 4.5 on kestose); ~ 1000 U/mg (40C, pH 4.5 on kestose).

RELATIVE RATES OF HYDROLYSIS OF SUBSTRATES:

Substrate Relative Hydrolysis Rate

Kestose 100Sucrose 143Raffinose 36Inulin (dahlia) 11

PHYSICOCHEMICAL PROPERTIES:pH Optima: 3.5 - 4.5 Temperature Optima: 50C - 60C

pH Stability: 3.0 - 9.0 Temperature Stability: up to 50C

Cat. Code: E-EXOIANContent: 5000 Units at 40CPrice: 127.00 / $173.00

Megazyme now offers highly pure, recombinant endo-Inulinase fromA. niger.Recombinant

Cat. code: E-ENDOIAN

(EC 3.2.1.7) CAZy: GH Family 32SPECIFIC ACTIVITY:

~ 580 U/mg (60C, pH 4.5 on inulin); ~ 300 U/mg (40C, pH 4.5 on inulin).

RELATIVE RATES OF HYDROLYSIS OF SUBSTRATES:

Substrate Relative Hydrolysis Rate

Inulin (Raftiline) (20 mg/mL) 100Inulin (dahlia) (10 mg/mL) ~ 95

PHYSICOCHEMICAL PROPERTIES:pH Optima: 4.5 - 5.5 Temperature Optima: 60C

pH Stability: 3.0 - 8.0 Temperature Stability: up to 40C

Cat. Code: E-ENDOIAN

Content: 500 Units at 40CPrice: 118.00 / $160.00

Enzymes & Kits for Fructan Analysis & Research


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Fructan Kit (K-FRUC) suitable for the measurement of even trace levels of fructan (inulin orfructo-oligosaccharides, FOS) in plant and food products e.g. in grain and in infant formulations. Theremoval of interfering sugars through borohydride reduction also reduces the terminal reducingfructose in partially hydrolysed fructans. This leads to an underestimation of up to 20% for highlydepolymerised fructans. However, if the pure FOS ingredient is available, the level of underestimation can

be determined and allowed for.Fructan HK (K-FRUCHK) gives no underestimation of fructan, even with highly depolymerisedsamples. However, the method is not recommended for samples containing high levels of D-glucose,D-fructose, sucrose and/or maltose.

Analytical Kits

S Fructan (K-FRUC)

- AOAC Method 999.03; AACC Method 32-32.01

- Incorporating highly puried enzymes including

recombinant exo-inulinase and endo-inulinase

S Fructan HK (K-FRUCHK)

- Incorporating highly puried enzymes including

recombinant exo-inulinase and endo-inulinase

- For quantitative measurement of fructan and FOS

Pure Research Enzymes

S exo-Inulinase (E-EXOIAN) (recombinant, afnity puried) NEW

S endo-Inulinase (E-ENDOIAN) (recombinant, afnity puried) NEW

Puried Enzymes for Specic Analytical Applications

S exo-Inulinase plus endo-Inulinase mixture (E-FRMXLQ)

- For measurement of fructan according to AOAC Method 997.08

S exo-Inulinase plus endo-Inulinase mixture (E-FRMXPD)- For measurement of fructan according to AOAC Method 997.08

S Amyloglucosidase (E-AMGDFR100 & E-AMGFR500)

- For use in fructan determination according to AOAC Method 997.08

Soluble Chromogenic Substrates

S Azo-Fructan (S-AZFR5)

- For the measurement ofendo-inulinase


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Isoamylase (E-ISAMY) Sfor complete debranching of starch and glycogen. S Ultrapure. Contaminating enzymes < 1 part in 106 (by activity).

a-Amylase (B. amyloliquefaciens)(E-BAASS) S for analysing the cluster structure of starches ( Bertoft et al, 2012*). S highly purified. Contaminating enzymes < 1 part in 106 (by activity).

* Bertoft, E., Koch, K. & Aman, P. (2012) Building block organisation of clusters in amylopectinsof different structural types, Int. J. Biol. Macromol., 50, 1212-1223.

Amyloglucosidase (Rhizopus sp.)(E-AMGPU)Sfor complete hydrolysis of soluble starch. No action on blocked substrates.

S Ultrapure. Contaminating enzymes < 1 part in 107 (by activity).

Amyloglucosidase (H. resinae)(E-GAMP)Sfor complete hydrolysis of soluble starch. No action on blocked substrates.

S Recombinant / affinity purified. Contaminating enzymes < 1 part in 107 (by activity).

a-Glucosidase (B. Stearothermophilus)(E-TSAGS) Srapidly hydrolyses all a-1,4-linked maltodextrins.

S Recombinant / affinity purified. Contaminating enzymes < 1 part in 107 (by activity).

a-Glucosidase (yeast)(E-MALTS)Srapidly hydrolyses maltose and maltotriose. Not active on a-1,6-linked D-glucose.

S Ultrapure. Contaminating enzymes < 1 part in 107 (by activity).

b-Amylase (barley)(E-BARBL) S removal of maltosyl units from the non-reducing ends of maltodextrin chains.

S Recrystallised. Contaminating enzymes < 1 part in 106 (by activity).

Pullulanase(E-PULKP)S for partial but controlled debranching of starch.

S Affinity purified. Contaminating enzymes < 1 part in 105 (by activity).

Pure Enzymes for Starch Research



newsletter jan-mar 2013

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