NEXTFLEX® RAPID XP DNA-SEQ KIT N O VA - 5 1 4 9 - 0 2 - P R O D U C T I N T R O D U C T I O N

Commercial launch on October 12, 2018 perkinelmer-appliedgenomics.com/rapid-xp

Catalog #NOVA-5149-02 (48 reactions)

List price USD1200

Robust DNA-seq kit that includes

enzymatic fragmentation to obtain

sequence-ready libraries from genomic

DNA using a streamlined protocol

NEXTFLEX® RAPID XP DNA-SEQ KIT XP STANDS FOR EXTRA PERFORMANCE

Reliable

Convenient

Flexible

Consistent fragmentation, library yields, and high-quality

sequencing data

All-inclusive kit that has been streamlined and automated

on Sciclone® and Zephyr® NGS workstations

Use with NEXTFLEX® color-balanced barcodes for

multiplexing up to 384 samples and PCR-free workflows

NEXTFLEX® RAPID XP DNA-SEQ KIT VALUE PROPOSITION

NEXTFLEX® RAPID XP DNA-SEQ KIT WORKFLOW

Manual (1.5 to 2.5 hours) Sciclone® workstation

Zephyr® workstation

NEXTFLEX® RAPID XP DNA-SEQ KIT CONSISTENT ENZYMATIC FRAGMENTATION

Figure 1: Reliable DNA enzymatic fragmentation with NEXTFLEX® Rapid XP

DNA-seq kit. Each dot indicates the library peak size. DNA is uniformly and

reproducibly fragmented into smaller sizes using the NEXTFLEX® Rapid XP DNA-

seq kit (457 ± 12 bp, c.v.=3 %, n=18). In comparison, the DNA libraries obtained

using Competitor K’s kit was non-uniform and variable indicated by the diffuse

pattern observed in the plot (730 ± 119 bp, c.v.=16 %, n=17). Libraries were

prepared from 100 ng of gDNA from 5 different commercially available sources

with 8 minutes of fragmentation and 5 cycles of PCR.

NEXTFLEX® RAPID XP DNA-SEQ KIT HIGH LIBRARY YIELD

Figure 2: High yield obtained for libraries prepared

using the NEXTFLEX® Rapid XP DNA-seq

kit. Libraries were prepared using both NEXTFLEX®

Rapid XP DNA-seq kit and Competitor K’s kit. Libraries

were prepared from 100 ng of DNA with 8 minutes of

fragmentation with 5 PCR cycles.

0

50

100

150

200

250

Human male Human mixed Jurkat Hela MCF7 Placenta

Yiel

d (

nM

)

NEXTFLEX® Competitor K

NEXTFLEX® RAPID XP DNA-SEQ KIT LOW GC BIAS

Figure 3. Libraries prepared with the

NEXTFLEX® Rapid XP DNA-seq kit show less or

comparable GC-bias than libraries prepared

with Competitor K’s kit. Picard GC bias analysis,

which show read coverage over the indicated

genome with respect to GC content, is shown as a

fraction of normalized coverage (blue) calculated in

100 bp windows (red) and plotted against the left y-

axis. Mean base quality at each window (green) is

calculated and plotted against the right y-axis.

Saccharomyces cerevisiae

NEXTFLEX®

Competitor K

Escherichia coli Bordetella pertussis

NEXTFLEX® RAPID XP DNA-SEQ KIT HIGH GENOME COVERAGE

Figure 4. Libraries prepared with the NEXTFLEX® Rapid XP DNA-seq kit show higher or comparable genome coverage

compared to libraries prepared using Competitor K’s kit. The percentage of genome bases covered at a minimum depth of 1X, 5X,

and 10X of S. cerevisiae, E. coli, and B. pertussis.

70 %

75 %

80 %

85 %

90 %

95 %

100 %

1 X 5 X 10 X

S. cerevisiae

NEXTFLEX® Competitor K

92 %

93 %

93 %

94 %

94 %

95 %

95 %

96 %

96 %

97 %

1 X 5 X 10 X

E. coli

NEXTFLEX® Competitor K

92 %

93 %

93 %

94 %

94 %

95 %

1 X 5 X 10 X

B. pertussis

NEXTFLEX® Competitor K

NEXTFLEX® RAPID XP DNA-SEQ KIT HIGH MAPPING RATE AND LOW DUPLICATION RATE

Figure 5. Libraries prepared with the NEXTFLEX® Rapid XP DNA-seq kit resulted in better or comparable data with respect to

mapping rate and duplication rate, compared to libraries prepared using Competitor K’s kit. (A) Mapping rates represented as the

percentage of reads which aligned to the indicated genome. (B) Duplication rates represented as the percentage of reads predicted to be PCR

duplicates by Picard MarkDuplicates.

0 %

10 %

20 %

30 %

40 %

50 %

60 %

70 %

80 %

90 %

100 %

S. cerevisiae E. coli B. pertussis

Mapping Rate

NEXTFLEX® Competitor K

0 %

1 %

2 %

3 %

4 %

5 %

6 %

7 %

8 %

S. cerevisiae E. coli B. pertussis

Duplication Rate

NEXTFLEX® Competitor K

NEXTFLEX® RAPID XP DNA-SEQ KIT POSITIVE EARLY ACCESS DATA

Successful displacement of incumbent chemistry

(Agilent® SureSelectXT®)

Compatibility with hybrid capture and multiple sample

types (saliva, whole blood, DBS, swab)

Fewer PCR cycles (less bias)

Robust Library yields reported from 50ng gDNA input

Site 1: Perkin Elmer Genomics

Site 2: Anonymous (customer site)

NEXTFLEX® RAPID XP DNA-SEQ KIT POSITIVE EARLY ACCESS DATA

Fewer steps => time and consumable savings Faster and more consistent fragmentation

Site 3: Perkin Elmer Health Sciences Australia

NEXTFLEX® Competitor K

12 minutes fragmentation 15 minutes fragmentation

XP produces tighter & more

consistent size distribution

compared with KAPA Hyperplus

with less incubation time.

Combined fragmentation and

ERAT reduces hands on time.

EXTERNAL POSITIONING: KAPA®

You Customer

Kapa® HyperPlus™ Library Prep Kit w amp NEXTFLEX® Rapid XP DNA-Seq Kit

$32 Price Per Sample (no barcodes, only kit) $25

No (~$3-$5 per prep) Beads included? Yes (No additional cost)

96 How many barcodes are available? 384

Two steps Fragmentation and end-repair/adenylation One step

50 ng Minimum Input amount complex genomes 1 ng

Variable (see supporting data in the appendix) Fragmentation efficiency Consistent (see data in the deck)

Reagent One-stop shop Automation + Reagent

I have been using Kapa

kits and am generally

happy with it.

I understand. Kapa is a widely-used kit. However, we have a new

compelling alternative that can save you money, time, and trouble.

Save >$10 prep sample (at list price)

Save on sequencing by higher multiplexing

More consistent enzymatic fragmentation

Input for complex genomes down to 1ng

Single point of contact for all library prep support

EXTERNAL POSITIONING: NEB®

You Customer

NEBNext® Ultra™ II FS DNA Library Prep with Sample Purification Beads NEXTFLEX® Rapid XP DNA-Seq Kit

$27.5 Price Per Sample (no barcodes, only kit) $25

96 How many barcodes are available? 384

No, indexed primers also increase index hopping PCR-free workflow Yes

Variable (see supporting data in the apendix) Operator repeatability Consistent

Reagent One-stop shop Automation + Reagent

I have been using

NEBNext kits and am

generally happy with it.

I understand. NEB is a high-performing kit. However, we

have a new compelling alternative that can save you

money, time, and offer flexibility.

Save >$2 prep sample prep (at list price)

Save on sequencing by higher multiplexing

PCR-free workflows

More consistent operator-to-operator reproducibility

Single point of contact for all library prep support

EXTERNAL POSITIONING: NEXTERA®

You Customer

I have been using Nextera

kits and am generally

happy with it.

Nextera® DNA and Nextera® XT DNAand Nextera® DNA Flex Library Preparation Kit NEXTFLEX® Rapid XP DNA-Seq

Kit

$34 or $82 Price Per Sample (no barcodes, only kit) $25

No Beads included? Yes (No additional cost)

No PCR-free workflow compatible? Yes

Locked at 1ng or 50ng only. High risk if badly quantified. (Flex allows 1-500ng) Range of Inputs? Yes

Variable Fragmentation efficiency and library yield Consistent (see data in the deck)

No, locked to tagmentation protocol (~250 or ~350 for Flex) Adjustable fragment size? Yes

>300bp, lose distal 50bp PCR Amplicon compatibility? Yes

Reagent One-stop shop Automation + Reagent

I understand. Illumina would be an obvious choice for library prep.

However, we have a new compelling alternative that can save you

money, time, trouble, and offer flexibility.

Save >$10 on prep sample (at list price)

Adjust fragmentation time and library size for different applications

Range of sample input concentration

PCR-free workflow

More consistent enzymatic fragmentation and high library yield

Single point of contact for all library prep support

NEXTFLEX® RAPID XP DNA-SEQ KIT DIFFERENCE BETWEEN RAPID XP DNA-SEQ AND RAPID DNA-SEQ KIT 2.0

NEXTFLEX® Rapid DNA-Seq 2.0 Kit NEXTFLEX® Rapid XP DNA-Seq Kit

Fragmentation Included? Not included, customer provided (Covaris® etc.) Included (enzymatic)

Turnaround Time ~2 to 3 hours (does not include fragmentation) ~1.5 to 2.5 hours (includes fragmentation)

Input Amount 1 ng to 1 ug 1 ng to 1 ug

# of Cleanup Steps 2 (1 if PCR-free workflow) 2 (1 if PCR-free workflow)

Barcodes Included? NEXTFLEX-HT™ barcodes (bundle) OR bundle

pricing (ex. NEXTFLEX® UDI) Bundle pricing, 50% off NEXTFLEX® barcodes

Beads Included? Yes Yes

Reliable

Convenient

Flexible

Consistent fragmentation, library yields and high-quality

sequencing data

All-inclusive kit that has been streamlined and automated

on Sciclone® and Zephyr® NGS workstations

Use with NEXTFLEX® color-balanced barcodes for

multiplexing up to 384 samples and PCR-free workflows

NEXTFLEX® RAPID XP DNA-SEQ KIT VALUE PROPOSITION

NEXTFLEX® RAPID XP DNA-SEQ KIT TARGET CUSTOMERS

Any DNA-seq application (WGS, WES, targeted seq, cDNA seq, PCR-free seq, etc.)

Tier 1: All Sciclone® and Zephyr® accounts

Tier 2: Core labs with high throughput that may benefit by replacing Covaris® with enzymatic

solution (drivers are performance and price)

Tier 3: Commercial and academic labs performing DNA-seq

Recommended Actions

Send product introduction email/collateral to Sciclone® and Zephyr® accounts

Use promotions to motivate customers to evaluate and switch

NEXTFLEX® RAPID XP DNA-SEQ KIT NEXTFLEX® PROMOS

NEXTFLEX® RAPID XP DNA-SEQ KIT SALES TOOL

Collateral Content Availability Location

Flyer General overview and selling points

October 12

BOX folder

Technical App Note Technical overview and competitor

comparison BOX folder

Internal Product Training Deck General overview and selling points BOX folder

Customer-facing Product Intro Deck General overview and competitive data BOX folder

Website Product page and ordering (SAP) Rapid-XP

User Guide/Manual User guide for library prep.

Instructions to obtain platform scripts BOX folder

Sales Training Product details Week of October 8 Webinar

NEXTFLEX® RAPID XP DNA-SEQ KIT QUESTIONS?

NGS@PerkinElmer.com

NEXTFLEX® RAPID XP DNA-SEQ KIT APPENDIX

NEXTFLEX® RAPID DNA-SEQ KIT 2.0 (NOT XP) COMPETITOR DETAILS

Kapa® HyperPrep™

Kit

Illumina® Nextera® or Nextera® XT DNA

Library Preparation Kit

Illumina® Nextera® DNA Flex Library Preparation Kit

Illumina® Truseq® Nano DNA Kits or

PCT-free Kits

NEBNext® Ultra™ II DNA Library Prep With Sample

Purification Beads

NEXTFLEX® Rapid DNA-Seq 2.0 kit

NEXTFLEX® Rapid DNA-Seq 2.0 Bundle

kit

Kit Price

$2,469 (96rxn), $720 (24rxn), $272

(8rxn)

$7870 (96rxn), $2200 (24rxn) OR $3270

(96rxn), $860 (24 rxn)

$4032 (96rxn), $1008 (24rxn)

$2650 (96rxn), $660 (24rxn)

$2465 (96rxn), $645 (24rxn) $2208 (96rxn), $1132 (48rxn), $194 (8rxn)

$2640 (96rxn), $1353 (48rxn), $232 (8rxn)

Price Per Sample (including barcode

purchase) $39 - $31

$85 - $94 OR $37 - $39

$47 $33 - $35 $30 - $45 $23 - $24 $27.5 - $29

Package Size (RXNs) 8, 24, 96 24, 96 24, 96 24, 96 24, 96 8, 48, 96 8, 48, 96

Fragmentation included? No Yes Yes No No No No

Beads included? No No No Yes Yes Yes Yes

Barcodes included? No No No No No No Yes (8, 48, 96 –HT

barcodes)

How many barcodes available?

24 SI or 96 DI 96 96 24 SI or 96 DI 12 SI or 96 DI 384 NA

Minimum input amount 1 ng 50 ng or 1ng 1 ng (rec 100ng) 100ng or 1ug 500 pg 1ng 1ng

Turnaround time ~2-3 hours ~1.5 hours ~1.5 hours ~5-6 hours ~2.5 hours ~3 hours ~3 hours

Yield +++ unknown unknown unknown ++++ ++++ ++++

*Pricing information and attributes found on vendor’s websites on 6-7-18

NEXTFLEX® RAPID XP DNA-SEQ KIT COMPETITOR DETAILS *Pricing information and attributes found on vendor’s websites on 10-6-18

Nextera® DNA Library

Preparation Kit

Nextera® XT DNA

Library Preparation Kit

Nextera® DNA Flex

Library Prepparation Kit

Kapa® HyperPlus™

Library Prep Kit w amp

NEBNext® Ultra™ II FS DNA

Library Prep for Illumina®

Qiagen® QIAseq® FX

DNA Library Kit

Swift® S2 Turbo™/Turbo

Flexible™ DNA Library Kits

NEXTFLEX® Rapid

XP DNA-Seq Kit

Kit Price (large size) $7,870 $3,270 $4,032 $3,072 $2,400 $3,280 $1,440 $1,200

Price Per Sample (no

barcodes, just kit) $82 $34 $42 $32 $25

$34 (includes

barcodes) $15 $25

Large Size (RXNs) 96 96 96 96 96 96 96 48

Fragmentation included? Yes Yes Yes Yes Yes Yes Yes Yes

Beads included? No No No for size selection No No No No Yes

Barcodes included? No No No No No Yes No No, price bundling

How many barcodes

available? 96 384 96 96 96 96 96 384

Minimum input amount 50 ng lock 1 ng lock

1ng (microbial), 100 ng

(complex and for

integrated normalization)

1 ng (microbial), 50 ng

(complex) 500 pg 1ng 1ng 1 ng

PCR-free workflow

compatible? No No No Yes No Yes No Yes

Turnaround time ~1.5 hours ~1.5 hours

~ 3-4 hours (INCLUDING

DNA extraction and

library normalization)

~1.5 to 2.5 hours ~2.5 hours ~2.5 hours ~1.5 to 2.5 hours ~1.5 – 2.5 hours

Notes:

Free-Adapter Blockers

(+ $).

Input is locked.

Not PCR-free

compatible.

For small genomes.

Free-Adapter Blockers

(+ $)

Input is 1ng only.

Not PCR-free

compatible.

Small, large, human

genomes

Blood, gDNA, Saliva

Bead-linked

transposome can do

tagmentation, index

tagging, normalization in

one step

Flex Lysis (for blood,

0.5hrs added only)

Market Leader.

Fragmentation and end-

repair/adenylation are

separate steps.

Quality brand.

Not PCR-free compatible,

barcoded primers contribute

significantly to index hopping

on patterned flow cells.

Includes barcode plate

with pierceable seal

for partial plate usage.

Up to 768-plex using

Combinatorial DI

(complicated)

Not PCR-free compatible,

barcoded primers contribute

significantly to index

hopping on patterned flow

cells.

Very EDTA sensitive

Sell together with

superior quality of our

barcodes.

Yield No notes/++ No notes/++ No notes/++ +++ ++++ No notes on yield No notes on yield +++

Kapa®’s Inconsistent Fragmentation

*from NEB® technical document available online 10-4-18. *from Agilent® technical document available online 10-4-18.

NEB®’s Variation by Operator

*from NEB® technical document available online 10-4-18. Same Control sample produced variable results for each User.

NEXTERA®’s Locked Tagmentation Protocols

*from NEB® technical document available online 10-4-18.

*from Illumina® technical document available online 10-5-18.