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The NII i Guidr :tnn(i~inc~s5cientific initlatnu and pro\idca policy mncl administrati\,ein5)rnm;ition t ( i indi\,i d u k and organizrrtions \Tho need t o he krpt inR)rnmed ~ i f o p p ~ i n u n ~ t ~ e a . rcquirenic.nt\, and changes in extra niuctl Imigranis ;idniintster~tJb!. the

-/ National Insritutes o f 1 Iealth

Vol. 17, No. 22 Ju ly 1, 1988

Vol. 17, N O . 22, J u l y 1 , 1988

NOTICES

AVAILABILITY OF RETINAL DEGENERATION MUTANTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1 e N a t i o n a l Eye I n s t i t u t e Index: EYE

THE ALCOHOL, DRUG ABUSE, AND MENTAL HEALTH ADMINISTRATION ESTABLISHES THE ADAMHA REVIEWERS RESERVE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 Alcohol , Drug Abuse, and Mental Hea l th A d m i n i s t r a t i o n Index: ALCOHOL, DRUG ABUSE, AND MENTAL HEALTH

DATED ANNOUNCEMENTS (RFPs AND RFAs AVAILABLE)

PRIMARY RODENT PRODUCTION CENTERS (RFP) ..................................... N a t i o n a l Cancer I n s t i t u t e

3

Index : CANCER

MICROSTIMULATION OF THE SACRAL SPINAL CORD (RFP) ............................ N a t i o n a l I n s t i t u t e of N e u r o l o g i c a l and Communicative D i s o r d e r s and S t r o k e

4

Index: NEUROLOGICAL AND COMMUNICATIVE DISORDERS AND STROKE

REVERSIBLE CONTRACEPTION AND RISK OF H I V INFECTION I N WOMEN (RFP) . . . . . . . . . . . 4 N a t i o n a l I n s t i t u t e o f Ch i ld Hea l th and Human DevelopmentIndex: C H I L D HEALTH AND HUMAN DEVELOPMENT

LIVER TRANSPLANTATION C O O R D I N A T I N G CENTER (RFP) ............................. 5 N a t i o n a l I n s t i t u t e o f D i a b e t e s , and D i g e s t i v e and Kidney Diseases Index: DIABETES, DIGESTIVE AND K I D N E Y DISEASES -LIVER TRANSPLANTATION CENTERS (RFP) ......................................... 5 N a t i o n a l I n s t i t u t e o f D i a b e t e s , and D i g e s t i v e and Kidney Diseases Index : DIABETES, DIGESTIVE ,AND K I D N E Y DISEASES

CHARACTERIZATION OF THE GENOMES OF HUMANS AND MODEL ORGANISMS (RFA) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6 N a t i o n a l I n s t i t u t e of General Medical S c i e n c e s Index : GENERAL MEDICAL SCIENCES

NEW TECHNOLOGY FOR GENOMIC ANALYSIS (RFA) ................................... 7 N a t i o n a l I n s t i t u t e o f General Medical S c i e n c e s Index: GENERAL MEDICAL SCIENCES

NOTICES

AVAILABILITY OF RETINAL DEGENERATION MUTANTS

P.T. 3 4 ; K.W. 0715100, 0780005, 1002002 Irr'

N a t i o n a l Eye I n s t i t u t e

The N a t i o n a l Eye I n s t i t u t e (NE11 s u p p o r t s t h e b reed ing and t h e d i s t r i b u t i o n of w e l l - c h a r a c t e r i z e d r e t i n a l d e g e n e r a t i o n mutants t o q u a l i f i e d i n v e s t i g a t o r s .Mutants (mice, ra ts , and dogs) and some l i m i t e d s e r v i c e s ( e . g . , t i s s u e p r e p a r a t i o n ) are p rov ided . There w i l l be no fee f o r an ima l s , t i s s u e s , o r s e r v i c e s , b u t a l l s h i p p i n g cha rges must be m e t by t h e i n v e s t i g a t o r . The NE1 program g o a l i s t o a c c e l e r a t e t h e pace o f r e s e a r c h on R e t i n i t i s Pigmentosa and o t h e r r e t i n a l d e g e n e r a t i v e d i s o r d e r s and t o a t t r a c t new i n v e s t i g a t o r s t o t h e f i e l d . Some s p e c i f i c s and t h e name o f t h e a p p r o p r i a t e pe r son t o c o n t a c t f o r complete i n f o r m a t i o n f o l l o w . NOTE: Guide format r e s t r i c t i o n s p r e c l u d e t h e u s e o f s u p e r s c r i p t s y b o l s ; g e n e t i c s u p e r s c r i p t symbols a r e p r i n t e d on t h e same l i n e as r e g u l a r Text, b u t are preceded by an a s t e r i s k ( X I .

i

MICE

Inb red l i n e s o f mice wi th i n h e r i t e d r e t i n a l d e g e n e r a t i o n s o r o t h e r v i s u a l system d e f e c t s a r e a v a i l a b l e from two s o u r c e s , t h e D i v i s i o n o f Neuroscience,C h i l d r e n ' s H o s p i t a l (Bos ton) and Erasmus U n i v e r s i t y (Rot te rdam) .

MICE (BOSTON COLONY)

The f o l l o w i n g t a b l e l i s t s t h e mutant s t r a i n s t h a t are a l r e a d y e s t a b l i s h e d and t h o s e t h a t w i l l become a v a i l a b l e s h o r t l y . A d d i t i o n a l hypopigmentat ion mutants and a l l e l e s w i l l b e added i f a demand f o r them i s e x p r e s s e d .

Name Symbol

A v a i l a b l e mutant s t r a i n s :

A 1b i n o cX2J Nervous n r P e a r l Pe

r r J pe+H

P e a r l ( w i l d t y p e - r e v e r t . ) pe*+Pin p e++2Pi n

Pink-eyed u n s t a b l e p+unP u r k i n j e c e l l d e g e n e r a t i o n pcd

R e t i n a l d e g e n e r a t i o n r d

Ruby-eye-2 ru-2XJ Flecked T(X;7)1Ct( C a t t a n a c h ' s t r a n s l o c a t i o n )

S t r a i n

C57BL/6J-c+2JBALBIcByJ-nrC57BL/6 J-pe C57BL16J P in-pe C57BL/6JPin-pe+HC57BL/6JPin-peX+PinC57BL/6JPin-peX+2PinC57BL/6J-p+unBALB/cByJ-pcdC 5 7BL/6J -pcdC3H/HeJC57BL/6J-rd l e Gus-snh C57BL/6J-rdC 5 7BL/6 J -ru-2* J Dp/X c+2J/Df

A d d i t i o n a l s t r a i n s t o be made a v a i l a b l e a t a l a t e r d a t e :

P u r k i n j e c e l l d e g e n e r a t i o n pcdn2J SM/J-pcdnZJP u r k i n j e c e l l d e g e n e r a t i o n C57BL/6J-pcd%2JP u r k i n j e c e l l d e g e n e r a t i o n pcd*Sid C57BL/6J-pcd%SidS t e e l D ick ie S lnd CS 7BL/ 6J V i t i l i g o v i t C57BL/6 J -v it

For f u r t h e r i n fo rma t ion , c o n t a c t :

D r . R ichard L. Sidman o r D r . Paul Neumann D i v i s i o n o f Neuroscience C h i l d r e n ' s H o s p i t a l 3 0 0 Longwood Avenue Boston, Massachuse t t s 02115 Telephone: (617) 735-6077 o r -6076

MICE (ROTTERDAM COLONY)

R e t i n a l d e g e n e r a t i o n ( r d ) and R e t i n a l degene ra t ion s low, ( r d s ) are a v a i l a b l e t q / s e p a r a t e l y and as a double mutant on t h e pigmented C3H-rd*+ and a l b i n o BALB/c

congenic l i n e s .

For f u r t h e r i n f o r m a t i o n c o n t a c t :

Vol . 17, No. 22, J u l y 1 , 1988 - Page 1

D r . Somes C . Sanyal Department o f Anatomy I Erasmus U n i v e r s i t y Postbox 1738 3 0 0 0 DR Rotterdam, The Ne the r l ands Telephone: 01-4635182

RATS

Royal Co l l ege o f Surgeons (RCS) r a t s w i t h i n h e r i t e d r e t i n a l dys t rophy and s e v e r a l congen ic s t r a i n s o f RCS an ima l s are a v a i l a b l e as f o l l o w s :

RCS Pink-eyed d y s t r o p h i c s t r a i n RCS -rdyXt Pink-eyed normal ( c o n t r o l ) s t r a i n RCS-pX+ Black-eyed d y s t r o p h i c s t r a i n RCS-rdyX+pX+ Black-eyed normal ( c o n t r o l ) s t r a i n RCS-c Albino d y s t r o p h i c s t r a i n

( A v a i l a b l e as non-congenic s t o c k 1988, congenic s t o c k , 1990)

Congenic F344-c/+ rats are a l s o a v a i l a b l e . T h i s s t r a i n p r o v i d e s g e n e t i c a l l y similar a l b i n o and hooded ( b l a c k pigmented) l i t t e r m a t e an ima l s w i t h normal r e t i n a s . These animals a re o p t i m a l f o r t h e s t u d y o f p igmen ta t ion d i f f e r e n c e s i n such r e s e a r c h areas as l i g h t - i n d u c e d r e t i n a l d e g e n e r a t i o n o r g a n g l i o n c e l l axona l gu idance mechanisms.

For f u r t h e r i n f o r m a t i o n , c o n t a c t :

D r . Matthew M . L a V a i l o r M s . Nancy Lawson U n i v e r s i t y o f C a l i f o r n i a , San F r a n c i s c o Department o f Anatomy, Box 0452 San F r a n c i s c o , C a l i f o r n i a 94143 Telephone: (415) 476-4234

DOGS

Animals and t i s s u e s are a v a i l a b l e on a c o m p e t i t i v e b a s i s . A b r i e f r e s e a r c h p r o t o c o l w i l l b e r e q u e s t e d and reviewed f o r s c i e n t i f i c merit by an independent a d v i s o r y commit tee . Approved i n v e s t i g a t o r s may e l e c t t o have dogs sh ipped t o t h e i r i n s t i t u t i o n o r t o have o c u l a r and/or nonocu la r t i s s u e s c o l l e c t e d and sh ipped from t h e co lony fo:llowing agreement on p r e s e r v a t i o n and s h i p p i n g p r o t o c o l s .

M i n i a t u r e pood les e i t h e r a f f e c t e d w i t h , he t e rozygous f o r , o r homozygous normal f o r p r o g r e s s i v e rod cone d e g e n e r a t i o n ( p r c d ) . The r e t i n a l d e g e n e r a t i o n i n p r c d - a f f e c t e d m . pood les i s a l a t e o n s e t d i s o r d e r c h a r a c t e r i z e d by a abnormally low r o d o u t e r segment (ROS) renewal r a t e .

I r i s h s e t t e r s e i t h e r a f f e c t e d w i t h , he t e rozygous f o r , o r homozygous normal f o r r o d cone d y s p l a s i a ( r c d l ) . The r e t i n a l d e g e n e r a t i o n i n r c d l - a f f e c t e d I r i s h se t t e r s i s an e a r l y o n s e t d i s o r d e r c h a r a c t e r i z e d by a r r e s t e d development o f ROS and abnormal r e t i n a l cycl ic GMP metabolism.

Norwegian elkhounds e i t h e r a f f e c t e d w i t h o r he t e rozygous f o r e a r l y r e t i n a l d e g e n e r a t i o n ( e r d ) . The co lony i s composed o f c r o s s b r e d elkhound-beagles , d e r i v e d from t h e o r i g i n a l pu reb red Norwegian elkhounds i n which t h e d i s e a s e e r d was f i rs t r e c o g n i z e d . The r e t i n a l d e g e n e r a t i o n i n e r d - a f f e c t e d dogs i s an e a r l y o n s e t d i s o r d e r c h a r a c t e r i z e d by abnormal development o f rod i n n e r and o u t e r segments and o f r o d and cone s y n a p t i c t e r m i n a l s . R e t i n a l c y c l i c GMP metabolism i s normal .

For f u r t h e r i n f o r m a t i o n , c o n t a c t :

D r . A n i t a A . Suran N a t i o n a l Eye I n s t i t u t e Nat iona l I n s t i t u t e s o f H e a l t h B u i l d i n g 31 , Room 6A49 Bethesda, Maryland 20892 Telephone: (301) 496-5983

Vol. 1 7 , No. 22, J u l y 1 , 1988 - Page 2

THE ALCOHOL, DRUG ABUSE, AND MENTAL HEALTH ADMINISTRATION ESTABLISHES THE ADAMHA REVIEWERS RESERVE

P.T. 34; K.W. 1014002

Alcohol , Drug Abuse, and Mental Hea l th Admin i s t r a t ion

'Iu# The Alcohol , Drug Abuse, and Mental Hea l th Admin i s t r a t ion (ADAMHA) is e s t a b l i s h i n g t h e ADAMHA Reviewers Reserve (ARR) , which w i l l become o p e r a t i o n a li n J u l y . ARR members w i l l be a b l e t o p a r t i c i p a t e i n t h e same manner as f u l l yappo in ted members i n meet ings o f ADAMHA's c h a r t e r e d i n i t i a l review groups( I R G s ) t h a t e v a l u a t e g r a n t and c o o p e r a t i v e agreement a p p l i c a t i o n s and r e s e a r c h and development c o n t r a c t p r o p o s a l s . The Office o f Ext ramura l Programs, Office o f t h e A d m i n i s t r a t o r , ADAMHA, w i l l manage t h e Reserve f o r agency-wide u s e . Nominations t o t h e Reserve w i l l be made by t h e t h r e e ADAMHA I n s t i t u t e s ,p r i m a r i l y from among t h e pool o f r e t i r i n g members o f c h a r t e r e d I R G s . On b e h a l f o f t h e A d m i n i s t r a t o r , ADAMHA, t h e I n s t i t u t e D i r e c t o r s w i l l se lect ,i n v i t e , and a p p o i n t h i g h l y q u a l i f i e d s c i e n t i s t s and o t h e r t e c h n i c a l e x p e r t s t o s e r v e on t h e Reserve . Appointment t o t h e Reserve may b e f o r up t o f o u r y e a r s a s l o n g as members f i l e and ma in ta in a c u r r e n t Form HHS 474 (S ta tement o f Employment and F i n a n c i a l I n t e r e s t s ) and do n o t a c c e p t appointment t o anyc h a r t e r e d Department o f Hea l th and Human S e r v i c e s p u b l i c a d v i s o r y commit tee . A t t h e r e q u e s t o f an Execut ive S e c r e t a r y , Reserve members may p a r t i c i p a t e w i t h up t o two c h a r t e r e d I R G s d u r i n g any one g r a n t review c y c l e ; however, t h e y may n o t s e r v e on t h e same IRG more t h a n twice i n one y e a r . The number o f Reserve members t h a t may p a r t i c i p a t e i n t h i s c a p a c i t y a t a g iven c h a r t e r e d IRG meet ingis l i m i t e d t o no more t h a n o n e l h a l f of t h e commit tee ' s quorum. A s i n t h e p a s t , Execu t ive S e c r e t a r i e s may a l s o i n v i t e ad hoc s p e c i a l r e v i e w e r s t o p rov ide a d v i c e and counse l t o c h a r t e r e d I R G s . However, ad hoc s p e c i a lr e v i e w e r s do n o t have t h e r i g h t s , p r i v i l e g e s , n o r o b l i g a t i o n s of appo in ted I R G o r Reserve members and may n o t o f f e r or v o t e on motions no r a s s i g n . p r i o r i t yr a t i n g s . The r o s t e r o f r ev iewers provided as p a r t o f t h e summary s t a t e m e n t ( p i n k s h e e t ) w i l l l i s t and s p e c i f y appo in ted I R G and ARR members and ad hoc c o n s u l t a n t s .

DATED ANNOUNCEMENTS (RFPs AND RFAs AVAILABLE)

PRIMARY RODENT PRODUCTION CENTERS

4,-J RFP AVAILABLE: NCI-CM-97575-72

P.T. 34; K.W. 1002002

N a t i o n a l Cancer I n s t i t u t e

The Developmental T h e r a p e u t i c s Program (DTP) o f t h e N a t i o n a l Cancer I n s t i t u t e ( N C I ) , N a t i o n a l I n s t i t u t e s o f Hea l th ( N I H ) , is seek ing o r g a n i z a t i o n s w i t h t h e c a p a b i l i t i e s and f a c i l i t i e s f o r producing l a r g e numbers o f i n b r e d r o d e n t s which are g e n e t i c a l l y sound and free o f pa thogen ic organisms. To b e c o n s i d e r e d f o r cont rac t a w a r d , o f f e r o r s shou ld m e e t t h e f o l l o w i n g c r i t e r i a : ( 1 ) t h e p r i n c i p a l i n v e s t i g a t o r and o t h e r key pe r sonne l must have expe r i enceand e x p e r t i s e i n t h e p roduc t ion of t h e h i g h e s t q u a l i t y r o d e n t s free from pa thogen ic organisms; ( 2 ) t h e f a c i l i t y must be a v a i l a b l e a t t h e time o f c o n t r a c t award, c a p a b l e o f producing h i g h e s t q u a l i t y r o d e n t s a t t a s k l e v e l s ;(3) o r g a n i z a t i o n a l expe r i ence i n p e r t i n e n t areas o f q u a l i t y r o d e n t p r o d u c t i o ni n c l u d i n g p e d i g r e e i n g p rocedures , i s o l a t o r p roduc t ion , e t c . , a t a scale commensurate w i t h t a s k performance; and ( 4 ) w i l l i n g n e s s t o p a r t i c i p a t e i n g r a n t e e reimbursement c o l l e c t i o n s . It is a n t i c i p a t e d t h a t t h r e e (3) awards w i l l be made a t t h e v a r i o u s t a s k l e v e l s . Only one ( 1 ) award w i l l be made t o any o r g a n i z a t i o n .

A l l i n t e r e s t e d o r g a n i z a t i o n s may submit w r i t t e n r e q u e s t s f o r c o p i e s o f t h e Request f o r P r o p o s a l s (RFP) N C I CM 97575-72. For ou r convenience , p l e a s ee n c l o s e two r e t u r n l a b e l s w i t h a l l r e q u e s t s . The RFP is scheduled t o be a v a i l a b l e on or about June 27, 1988, w i th a d e a d l i n e f o r r e c e i p t o f p r o p o s a l s on o r about August 15, 1988. A l l r e s p o n s i b l e o f f e r o r s may submit p r o p o s a l sf o r c o n s i d e r a t i o n by t h e N a t i o n a l Cancer I n s t i t u t e . For f u r t h e r i n fo rma t ion r e g a r d i n g t h i s s o l i c i t a t i o n p l e a s e d i r e c t a l l cor respondence t o :

Ms. J a c q u e l i n e B a l l a r d Con t rac t S p e c i a l i s tTreatment C o n t r a c t s S e c t i o n Research C o n t r a c t s Branch

'-f Nat iona l Cancer I n s t i t u t e Bla i r Bu i ld ing , Rm. 224 Bethesda, Maryland 20892

Vol . 17, No. 22, J u l y 1 , 1988 - Page 3

MICROSTIMULATION OF THE SACRAL SPINAL CORD

RFP AVAILABLE: NIH-NINCDS-88-15

P.T. 34; K.W. 0740050

N a t i o n a l I n s t i t u t e o f Neuro log ica l and Communicative D i s o r d e r s and S t r o k e . -The N a t i o n a l I n s t i t u t e o f Neuro log ica l and Communicative D i s o r d e r s and S t r o k e h a s a requi rement t o i n v e s t i g a t e t h e f e a s i b i l i t y o f m i c r o s t i m u l a t i o n o f t h e sacral s p i n a l c o r d as a method o f c o n t r o l l i n g m i c t u r i t i o n and s e x u a l f u n c t i o n s .

O f f e r o r s shou ld have expe r i ence i n e l e c t r o p h y s i o l o g y i n c l u d i n g e l ec t r i ca l s t i m u l a t i o n o f n e u r a l t i s s u e .

T h i s is an announcement of an a n t i c i p a t e d Request f o r P r o p o s a l s . RFP-NIH-NINCDS-88-15 w i l l be i s s u e d on o r about August 1 , 1988, w i t h a c l o s i n g d a t e f o r r e c e i p t o f p r o p o s a l s s e t f o r September 3 0 , 1988.

To r e c e i v e a copy o f t h e RFP, p l e a s e supp ly t h i s o f f i c e w i t h two s e l f - a d d r e s s e d m a i l i n g l a b e l s . A l l r e s p o n s i b l e s o u r c e s may submit a p roposa l which w i l l be c o n s i d e r e d by t h e agency. The RFP w i l l be a v a i l a b l e uponw r i t t e n r e q u e s t t o :

C o n t r a c t i n g Officer C o n t r a c t s Management Branch, NINCDS N a t i o n a l I n s t i t u t e s o f Hea l th F e d e r a l Bu i ld ing , Room 901 Bethesda, Maryland 20892

REVERSIBLE CONTRACEPTION AND RISK OF H I V INFECTION I N WOMEN

RFP AVAILABLE: NICHD-CE-88-12

P.T. 34; K.W. 0750020, 0411005, 0715120

N a t i o n a l I n s t i t u t e o f Ch i ld Hea l th and Human Development

The C o n t r a c e p t i v e E v a l u a t i o n Branch of t h e Cen te r f o r P o p u l a t i o n Research , N I C H D , h a s a requi rement f o r a c a s e - c o n t r o l s t u d y o f t h e r e l a t i o n s h i p s between -4-

r e v e r s i b l e c o n t r a c e p t i v e methods and i n c i d e n t H I V i n f e c t i o n i n s e x u a l l y a c t i v e women o f r e p r o d u c t i v e age w i t h no r i s k f a c t o r s f o r H I V i n f e c t i o n o t h e r t h a n v a g i n a l i n t e r c o u r s e . The o b j e c t i v e s o f t h e s t u d y are t o de t e rmine :

( 1 ) R e l a t i v e r i s k of i n c i d e n t H I V i n f e c t i o n i n women who are c u r r e n t l yu s i n g v a r i o u s r e v e r s i b l e c o n t r a c e p t i v e methods when compared t o non-con t racep to r s . Although a f u l l c o n t r a c e p t i v e h i s t o r y w i l l be o b t a i n e d , s p e c i f i c a t t e n t i o n w i l l be p a i d t o t h e u s e o f condoms,spe rmic ides and o r a l c o n t r a c e p t i v e s .

(2) The e f fec ts o f s e x u a l l y t r a n s m i t t e d d i s e a s e s and p e l v i ci n f l a m a t o r y d i s e a s e ( P I D ) on t h e r e l a t i v e r i s k s de te rmined f o r ( 1 )above.

The r e s u l t s from t h i s s t u d y w i l l p rov ide N I C H D w i t h in fo rma t ion n e c e s s a r y f o r p r e p a r i n g g u i d e l i n e s f o r t h e use o f c o n t r a c e p t i v e methods i n t h e c o n t r o l o f t h e AIDS epidemic .

T h i s announcement is n o t a r e q u e s t f o r p r o p o s a l s (RFP). RFP-NICHD-CE-88-12 w i l l be i s s u e d on o r about June 3 0 , 1988. P roposa l s w i l l be due approximate ly 120 days t h e r e a f t e r . Copies o f t h e RFP may be o b t a i n e d by send ing a w r i t t e n r e q u e s t t o t h e f o l l o w i n g a d d r e s s . P l e a s e e n c l o s e a s e l f - a d d r e s s e d l a b e l .

Paul J . Duska, C o n t r a c t i n g Officer C o n t r a c t s Management S e c t i o n , OGC N a t i o n a l I n s t i t u t e o f Ch i ld Hea l th and Human DevelopmentExecu t ive P l a z a North, Roam 610 Bethesda , Maryland 20892

Vol . 17, No. 22, J u l y 1 , 1988 - Page 4

LIVER TRANSPLANTATION COORDINATING CENTER

RFP AVAILABLE: RFP-NIH-NIDDK-88-3

P.T. 34; K.W. 0745015, 1004008

N a t i o n a l I n s t i t u t e o f D iabe te s , and D i g e s t i v e and Kidney Diseases

The N a t i o n a l I n s t i t u t e of D i a b e t e s and D i g e s t i v e and Kidney Diseases ( N I D D K ) h a s a requirement t o s u p p o r t a f u l l y o p e r a t i o n a l l i v e r t r a n s p l a n t a t i o n d a t a b a s e f o r t h e c o l l e c t i o n of d a t a from p a t i e n t s i n t h e United S t a t e s who have been e v a l u a t e d and have had l i v e r t r a n s p l a n t a t i o n f o r a v a r i e t y o f end- s t age l i v e r d i s e a s e s . The o v e r a l l g o a l o f t h i s L i v e r T r a n s p l a n t a t i o n Database i s t o answer impor t an t r e s e a r c h q u e s t i o n s about l i v e r t r a n s p l a n t a t i o n . T h i s p r o j e c t w i l l c o n s i s t of c o n t r a c t s f o r approx ima te ly 5 L i v e r T r a n s p l a n t a t i o n C e n t e r s and one Coord ina t ing C e n t e r . T h i s O p e r a t i o n a l Phase w i l l b e subd iv ided i n t o t h e f o l l o w i n g segments: a 3-month t r a n s i t i o n phase w i l l a l l o w time f o r any n e c e s s a r y m o d i f i c a t i o n of t h e d a t a c o l l e c t i o n forms and p rocedures developed i n t h e developmental and p i l o t phases ; t h e r e c r u i t m e n t segment is planned t o l a s t 3 y e a r s o r u n t i l a t l ea s t 750 p a t i e n t s and no more t h a n 1500 p a t i e n t s who r e c e i v e l i v e r t r a n s p l a n t a t i o n have been e n t e r e d i n t o t h e d a t a b a s e ; t h e follow-up phase w i l l a l l o w f o r t h e follow-up o f a l l p a t i e n t s e n t e r e d i n t o t h e d a t a b a s e f o r a n a d d i t i o n a l 2 y e a r s ; and t h e a n a l y s i s phase w i l l a l l o w 2 y e a r s f o r t h e complet ion o f d a t a a n a l y s i s and t h e s t o r a g e of d a t a .

During t h i s o p e r a t i o n a l phase, t h e Coord ina t ing Cen te r and T r a n s p l a n t a t i o n C e n t e r s w i l l b e invo lved i n app ly ing t h e methodology developed d u r i n g t h e p r e c e d i n g development and p i l o t phases .

The Coord ina t ing Cen te r w i l l b e r e s p o n s i b l e f o r mon i to r ing t h e d a t a c o l l e c t i o n and r e c o r d i n g , e d i t i n g , s t o r i n g , and a n a l y z i n g a l l o f t h e d a t a , and f o r m a i n t a i n i n g t h e d a t a c o l l e c t i o n system used by t h e T r a n s p l a n t a t i o n C e n t e r s . The Coord ina t ing Cen te r w i l l work w i t h each T r a n s p l a n t a t i o n Cen te r t o a s s u r e t h a t t h e d a t a i s c o l l e c t e d a c c o r d i n g t o s t a n d a r d p r o c e d u r e s . I t w i l l a n a l y z e t h e d a t a t o monitor t h e p r o g r e s s of t h e p r o j e c t and t o answer r e s e a r c h q u e s t i o n s about l i v e r t r a n s p l a n t a t i o n .

T h i s Request f o r P r o p o s a l s , RFP No. N I H - N I D D K - 8 8 - 3 , w i l l b e i s s u e d on o r about J u l y 1 1 , 1988, w i t h a c l o s i n g d a t e s e t f o r October 11, 1988.

To r e c e i v e a copy o f t h i s RFP, p l e a s e supp ly t h i s o f f i c e w i t h two s e l f - a d d r e s s e d m a i l i n g l a b e l s and c i t e t h e RFP number r e f e r e n c e d above. Reques t s must be i n w r i t i n g and addres sed t o :

S h i r l e y A . Shores C o n t r a c t s Management Branch ~N a t i o n a l I n s t i t u t e o f D i a b e t e s and D i g e s t i v e and KidneyD i s e a s e s Westwood B u i l d i n g , Room 602 Bethesda, Maryland 20892

Telephone r e q u e s t s w i l l n o t be honored. A r e a s o n a b l e number o f t h e RFP h a s been p r e p a r e d and w i l l b e i s s u e d on an a s - a v a i l a b l e b a s i s . T h i s a d v e r t i s e m e n t does n o t commit t h e Government t o make a n award.

LIVER TRANSPLANTATION CENTERS

RFP AVAILABLE: RFP-NIH-NIDDK-88-4

P.T. 04; K . W . 0745065, 0785035, 0785085

N a t i o n a l I n s t i t u t e o f D i a b e t e s , and D i g e s t i v e and Kidney Diseases

The N a t i o n a l I n s t i t u t e o f D i a b e t e s and D i g e s t i v e and Kidney Diseases ( N I D D K ) h a s a r equ i r emen t t o s u p p o r t a f u l l y o p e r a t i o n a l l i v e r t r a n s p l a n t a t i o n d a t a b a s e f o r t h e c o l l e c t i o n o f d a t a from p a t i e n t s i n t h e United S t a t e s who have been e v a l u a t e d and have had l i v e r t r a n s p l a n t a t i o n f o r a v a r i e t y o f end- s t age l i v e r d i s e a s e s . The o v e r a l l g o a l o f t h i s L ive r T r a n s p l a n t a t i o n Database is t o answer impor t an t r e s e a r c h q u e s t i o n s about l i v e r t r a n s p l a n t a t i o n . T h i s p r o j e c t w i l l c o n s i s t o f c o n t r a c t s f o r approx ima te ly 5 L i v e r T r a n s p l a n t a t i o n C e n t e r s and one Coord ina t ing C e n t e r . T h i s O p e r a t i o n a l Phase w i l l be subd iv ided i n t o t h e f o l l o w i n g segments: a 3-month t r a n s i t i o n phase w i l l a l l o w t i m e f o r any n e c e s s a r y m o d i f i c a t i o n o f t h e d a t a c o l l e c t i o n forms and p r o c e d u r e s developed i n t h e developmental and p i l o t phases ; t h e r e c r u i t m e n t segment i s planned t o l a s t 3 y e a r s o r u n t i l a t l e a s t 750 p a t i e n t s

Vol. 17, No. 22, J u l y 1 , 1988 - Page 5

and no more t h a n 1500 p a t i e n t s who r e c e i v e l i v e r t r a n s p l a n t a t i o n have been e n t e r e d i n t o t h e d a t a b a s e ; t h e follow-up phase w i l l a l l ow f o r t h e follow-up o f a l l p a t i e n t s e n t e r e d i n t o t h e d a t a b a s e f o r an a d d i t i o n a l 2 y e a r s ; and t h e a n a l y s i s phase w i l l a l l o w 2 y e a r s f o r t h e complet ion o f d a t a a n a l y s i s and t h e s t o r a g e o f d a t a .

During t h i s o p e r a t i o n a l phase, t h e Coord ina t ing Cen te r and T r a n s p l a n t a t i o nC e n t e r s w i l l b e invo lved i n a p p l y i n g t h e methodology developed d u r i n g t h e p r e c e d i n g development and p i l o t p h a s e s .

The T r a n s p l a n t a t i o n C e n t e r s w i l l b e r e s p o n s i b l e f o r p a t i e n t r e c r u i t m e n t and f o r c o l l e c t i n g t h e r e q u i r e d d a t a and s u b m i t t i n g it t o t h e Coord ina t ing C e n t e r . These C e n t e r s w i l l be expec ted t o r e c r u i t a s u f f i c i e n t number of p a t i e n t se v a l u a t e d f o r l i v e r t r a n s p l . a n t a t i o n t o p r o v i d e t h e L i v e r T r a n s p l a n t a t i o nDatabase w i t h a t l ea s t one l i v e r t r a n s p l a n t a t i o n p a t i e n t a week o r a t l ea s t 50 l i v e r t r a n s p l a n t a t i o n p a t i e n t s a y e a r . The T r a n s p l a n t a t i o n C e n t e r s w i l l a l s o be r e s p o n s i b l e f o r c o l l e c t i n g d a t a on t h e donor and on t h e h a r v e s t i n gprocedure .

T h i s Request f o r P r o p o s a l s , RFP No. NIH-NIDDK-88-4, w i l l b e i s s u e d on o r about J u l y 11, 1988, w i t h a c l o s i n g d a t e s e t f o r October 1 1 , 1988.

To r e c e i v e a copy o f t h i s RFP, p l e a s e supp ly t h i s o f f i c e w i t h two s e l f - a d d r e s s e d m a i l i n g l a b e l s and c i t e t h e RFP number r e f e r e n c e d above. Reques t s must be i n w r i t i n g and a d d r e s s e d t o :

S h i r l e y A. Shores C o n t r a c t s Management Branch N a t i o n a l I n s t i t u t e o f D i a b e t e s and D i g e s t i v e and KidneyDiseases Westwood B u i l d i n g , Room 602 Bethesda, Maryland 20892

Telephone r e q u e s t s w i l l n o t be honored. A r e a s o n a b l e number of t h e RFP h a s been p r e p a r e d and w i l l b e . issued on an as a v a i l a b l e b a s i s . T h i s a d v e r t i s e m e n t does n o t commit t h e Government t o make an award.

CHARACTERIZATION OF THE GENOMES OF HUMANS AND MODEL ORGANISMS

RFA AVAILABLE: 88-GM-02 U t

P.T. 3 4 ; K.W. 1002019, 0755045

N a t i o n a l I n s t i t u t e o f General Medical S c i e n c e s

A p p l i c a t i o n Rece ip t Date : September 22, 1988

BACKGROUND INFORMATION

The N a t i o n a l I n s t i t u t e o f General Medical S c i e n c e s (NIGMS) s u p p o r t s r e s e a r c h i n t h e f i e l d o f genomic a n a l y s i s , w i t h t h e g o a l o f deve lop ing d e t a i l e d g e n e t i cand p h y s i c a l maps and, e v e n t u a l l y , de t e rmin ing t h e complete sequence o f t h e DNA o f a number o f organisms, i n c l u d i n g man. I t is expec ted t h a t t h i s knowledge w i l l u l t i m a t e l y be a p p l i e d t o t h e p r e v e n t i o n , d i a g n o s i s , and t r e a t m e n t o f human d i s o r d e r s . The o b j e c t i v e o f t h i s Request f o r A p p l i c a t i o n s(RFA) i s t o s t i m u l a t e i n n o v a t i v e r e s e a r c h t h a t w i l l r a p i d l y improve ou r a b i l i t y t o a n a l y z e t h e e n t i r e genome o f a n organism.

RESEARCH GOALS AND SCOPE

The NIGMS i n v i t e s a p p l i c a t i o n s from i n t e r e s t e d i n v e s t i g a t o r s f o r r e s e a r c h which i n v o l v e s t h e f u r t h e r development o f p h y s i c a l maps o f t h e human genome;development o f p h y s i c a l maps and/or d e t e r m i n a t i o n o f t h e DNA sequence o f t h e genome o f one o f t h e f o l l o w i n g model organisms: y e a s t , Drosoph i l a , mouse o r t h e nematode worm, C a e n o r h a b d i t i s e l e g a n s ; o r development o f new approachesf o r d e t e r m i n a t i o n o f such mapping o r sequence i n f o r m a t i o n . [Research p r o j e c t sd i r e c t e d toward t h e s e g o a l s b u t which u t i l i z e o t h e r organisms are a p p r o p r i a t e l y submi t t ed i n r e sponse t o a n e x i s t i n g N I H Program Announcement ( N I H Guide f o r G r a n t s and C o n t r a c t s , V o l . 16, No. 18, p . 1 1 ; May 27, 198711.

To be c o n s i d e r e d r e s p o n s i v e t o t h i s RFA, p r o p o s a l s must be p r i m a r i l y d i r e c t e d toward t h e development of new mapping and sequencing d a t a o r t h e developmento f new me thodo log ica l approaches which w i l l i n c r e a s e t h e accu racy , ease, and r a p i d i t y w i t h which such mapping and sequence d e t e r m i n a t i o n can be a c h i e v e d . I n n o v a t i v e approaches t o o b t a i n i n g such i n f o r m a t i o n are encouraged.U t i l i z a t i o n o f p h y s i c a l mapping and DNA sequence d a t a f o r t h e a n a l y s i s and

Vol . 17, No. 22, J u l y 1 , 1988 - Page 6

c h a r a c t e r i z a t i o n o f genomic i n f o r m a t i o n is a l s o encouraged as p a r t o f t h e proposed p r o j e c t s . However, a p p l i c a t i o n s i n which t h e pr imary g o a l i s t h e s t u d y o f t h e b i o l o g y o f s p e c i f i c genomic r e g i o n s ( p a r t i c u l a r l y r e g i o n s r e l a t e d t o d i s e a s e g e n e s ) , and i n which mapping o r sequence d a t a are t o be o b t a i n e d o n l y as a n e c e s s a r y f i rs t s t e p toward t h a t end, w i l l n o t be c o n s i d e r e d r e s p o n s i v e t o t h i s announcement.

MECHANISM OF SUPPORT

Support w i l l be th rough r e s e a r c h g r a n t s i n c l u d i n g i n d i v i d u a l p r o j e c t s g r a n t s (R01, R29) and program p r o j e c t s ( P o l ) . The t o t a l amount o f s u p p o r t f o r g r a n t s under t h i s RFA is c o n t i n g e n t upon t h e a p p r o p r i a t i o n o f funds f o r t h i s pu rpose . The number o f awards w i l l be determined by t h e merit o f t h e p r o p o s a l s , by t h e i r r e l e v a n c e t o t h e program g o a l s , and a v a i l a b i l i t y o f f u n d s . I t is a n t i c i p a t e d t h a t i n F i s c a l Year 1989 up t o f i v e m i l l i o n d o l l a r s w i l l b e a l l o c a t e d t o t h e r e s e a r c h i n i t i a t i v e s d e s c r i b e d i n t h i s RFA, a l l o w i n g approx ima te ly 10-30 awards t o be made. T h i s amount may be i n c r e a s e d i f a l a r g e number o f h i g h l y m e r i t o r i o u s a p p l i c a t i o n s are r e c e i v e d and i f funds are a v a i l a b l e .

STAFF CONTACT

A p p l i c a n t s shou ld r e q u e s t t h e complete RFA and o b t a i n a d d i t i o n a l i n f o r m a t i o n from:

D r . Mark Guyer o r D r . I r e n e Eckstand N a t i o n a l I n s t i t u t e o f General Medical S c i e n c e Westwood B u i l d i n g , Room 918 N a t i o n a l I n s t i t u t e o f Hea l th Bethesda, Maryland 20892 Telephone: ( 3 0 1 ) 496-7137

NEW TECHNOLOGY FOR G E N O M I C ANALYSIS

RFA AVAILABLE: 88-GM-03

P . T . 34; K . W . 1002019, 0755045, 1004000, 0735000

N a t i o n a l I n s t i t u t e o f General Medical S c i e n c e s

A p p l i c a t i o n Rece ip t Date: December 1 , 1988

BACKGROUND INFORMATION

The N a t i o n a l I n s t i t u t e o f General Medical S c i e n c e (NIGMS) s u p p o r t s r e s e a r c h i n b a s i c g e n e t i c s , i n c l u d i n g r e s e a r c h d i r e c t e d toward t h e c o n s t r u c t i o n o f d e t a i l e d g e n e t i c and p h y s i c a l maps o f t h e genomes o f ' a number o f organisms,i n c l u d i n g man, and t h e d e t e r m i n a t i o n o f t h e complete sequence o f t h e DNA o f t h o s e organisms. T h i s knowledge w i l l u l t i m a t e l y be a p p l i e d i n b iomed ica l r e s e a r c h f o r t h e d i a g n o s i s , t r e a t m e n t , and p r e v e n t i o n o f human d i s e a s e . S i g n i f i c a n t advances have been m a d e d u r i n g t h e p a s t decade i n t h e t e c h n i q u e sa v a i l a b l e f o r d e t e r m i n a t i o n o f genomic s t r u c t u r e and o r g a n i z a t i o n and of n u c l e i c ac id sequences . The o b j e c t i v e o f t h e Request f o r A p p l i c a t i o n s (RFA)i s t o s t i m u l a t e i n n o v a t i v e r e s e a r c h t h a t w i l l l e a d t o f u r t h e r improvements i n t h e speed and e f f i c i e n c y o f map c o n s t r u c t i o n and DNA sequence d e t e r m i n a t i o n , so t h a t major r e s e a r c h e f f o r t s such as de te rmin ing t h e e n t i r e DNA sequence o f any organism w i l l become p r a c t i c a l i n terms o f b o t h t i m e and c o s t .

RESEARCH GOALS AND SCOPE

The NIGMS i n v i t e s a p p l i c a t i o n s from i n v e s t i g a t o r s f o r r e s e a r c h t h a t w i l l l e a d t o new o r improved t echno logy a p p l i c a b l e t o genomic a n a l y s i s . The o b j e c t of t h i s program i s t o make s u b s t a n t i a l improvements i n t h e r a p i d i t y , e f f i c i e n c yand accuracy w i t h which g e n e t i c mapping, p h y s i c a l mapping, and DNA sequencein fo rma t ion can be o b t a i n e d , ana lyzed , and d i s t r i b u t e d . M u l t i - d i s c i p l i n a r yapproaches t o t h e a t t a i n m e n t o f t h e s e g o a l s are encouraged; i n a d d i t i o n t o s c i e n t i s t i n a l l areas o f t h e b i o l o g i c a l s c i e n c e s , w e encourage t h e p a r t i c i p a t i o n o f s c i e n t i s t i n d i s c i p l i n e s which have n o t p r e v i o u s l y r e c e i v e d major amounts o f s u p p o r t from t h e N a t i o n a l I n s t i t u t e o f Hea l th ( N I H ) , such as computer s c i e n c e , materials s c i e n c e , p h y s i c s , mathematics and e n g i n e e r i n g .

MECHANISM OF SUPPORT

Support w i l l b e th rough r e s e a r c h g r a n t s i n c l u d i n g i n d i v i d u a l p r o j e c t g r a n t s( R 0 1 , R29) and program p r o j e c t s g r a n t s ( P o l ) . The t o t a l amount o f s u p p o r t f o r g r a n t s under t h i s RFA i s c o n t i n g e n t upon t h e a p p r o p r i a t i o n o f funds f o r t h i s pu rpose . The number o f awards w i l l b e determined by t h e meri t o f t h e

Vol. 17, N O . 22, J u l y 1 , 1988 - Page 7

p r o p o s a l s and by t h e r e l e v a n c e t o t h e program g o a l s , as w e l l as by a v a i l a b i l i t y o f f u n d s . It i s a n t i c i p a t e d t h a t i n F i s c a l Year 1989 up t o f i v e m i l l i o n d o l l a r s w i l l b e a l l o c a t e d t o t h e r e s e a r c h i n i t i a t i v e s d e s c r i b e d i n t h i s RFA, a l l o w i n g approx ima te ly 10-30 awards t o be made. T h i s amount may be i n c r e a s e d i f a l a r g e number of h i g h l y m e r i t o r i o u s a p p l i c a t i o n s are r e c e i v e d and i f funds are a v a i l a b l e .

STAFF CONTACT

A p p l i c a n t s shou ld r e q u e s t t h e complete RFA and o b t a i n a d d i t i o n a l i n f o r m a t i o n from:

D r . Mark Guyer o r D r . I r e n e Ecks t r and N a t i o n a l I n s t i t u t e o f General Medical S c i e n c e s Westwood B u i l d i n g , Room 918 N a t i o n a l I n s t i t u t e s of Hea l th Bethesda, Maryland 20892 Telephone: ( 3 0 1 ) 496-7137

II

Vol. 17, No. 22, J u l y 1 , 1988 - Page 8 *u. 5.GOVERNllENT P R I N T I N G OFFICE :19B8- 201-770r80002