nihms400638 supplement 03

8/11/2019 NIHMS400638 Supplement 03

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Supplemental Table 3 related to figure 4. Lineage tracing

gene translational patterndie-1 ubiquitous and germline expressed; degraded at morphogenesislin-13 ubiquitous and germline expressed; subnuclear foci

sdc-2 ubiquitous and germline expressed; becoming focal within nucleushlh-1 muscle precursorspha-4 pharyngeal, intestinal and hindgut cellsceh-16 lateral row of cellsceh-27 subset of pharyngeal and neuronal precursorscnd-1 lateral neuronal precursorsegl-5 C lineage muscle subsetelt-2 intestinal precursorselt-3 hypodermal p recursorsglp-1 highly patterned, primarily membrane-localizedlin-12 highly patterned, primarily membrane-localizedlin-39 lateral blast cell and C lineage subsetmed-2 EMS progenynhr-2 broadly expressed (most cells) from 24-100 cell stage, then degradedsea-1 Patterned in early embryos, then becoming ubiquitoustbx-8 highly patternedunc-130 head cells


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transcriptional pattern Match Additional informationubiquitous; not germline expressed yes + germ regulated degradationubiquitous in embryo, not germline expressed yes + germ subcellular localization

ubiquitous; not germline expressed yes + germ subcellular localizationmuscle precursors and other MS cells yes increased specificity of patternpharyngeal, intestinal and hindgut cells yes regulated degradationlateral row of cells yessubset of pharyngeal and neuronal precursors yeslateral neuronal precursors yes regulated degradationC lineage muscle subset yesintestinal precursors yes onset timehypodermal precursors yeshighly patterned yes subcellular localizationhighly patterned yes subcellular localizationlateral blast cell and C lineage subset yesEMS progeny yes degradationubiquitous yesUbiquitous with quantitative patterning yeshighly patterned yeshead cells yes


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ene strainGenes with movies and

alr-1 RW10757ama-1 RW10424

ceh-14 RW10726

ceh-26 RW10913

ceh-6 RW10871cnd-1 RW10434cnd-1 RW10435

die-1 RW10376

die-1 RW11053

e l-5 RW10600e l-5 RW10867elt-2 RW10714eor-1 RW10868fkh-4 RW10914hlh-1 RW10481hnd-1 RW10196lin-11 RW10493lin-13 RW10560lin-39 RW10349lir-2 RW11163

mab-5 RW10350med-2 RW10479me -1 RW11050nhr-2 RW11045

nhr-23 RW11070nhr-25 RW10231nhr-25 RW10348

pal-1 RW10889

pal-1 RW10993

ha-4 RW10425tbx-8 RW10558ttx-3 RW10713

unc-130 RW10866unc-130 RW11144


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Ex ression summar raced linea es

L-R As mmetric in C and MS ex ressedin GLR recursors and several neuro enic and h odermal ABUbi uitousNo expression by 350 cell stage; expressing cells at comma identified as VR, PVT, DVC, one

har n eal land cell and AFD neuronsExcretor cell and RMEV

Several AB linea es includin rectal and excretor recursors, SMBV and dorsal motor neuronsD namic in AB neuro enic linea es, ersistent in subset of DD motorneuronsD namic in AB neuro enic linea es, ersistent in subset of DD motorneuronsWeak maternal expression becoming bright around 200 cell stage and then lost except in subset ofhead neurons h odermal and har n eal cellsWeak maternal expression becoming bright around 200 cell stage and then lost except in subset ofhead neurons, h odermal and har n eal cellsPosterior C muscle, rectal cell UPosterior C muscleE linea eUbi uitous with bri hter ex ression in some neuronal linea esUbi uitous for short tem oral window at 28-50 cell sta eMost muscle recursorsMost muscle recursorsNo ex ression b 350 cell sta e ex ressed b comma in several likel neuro enic cellsUbi uitous with bri ht subnuclear foci in some cellsMedial to anterior P cells, seam cells, C h odermal and C muscle cellsUbi uitousMedial to osterior P cells, seam cells, C h odermal and C muscle cellsWeak in E, MSUbi uitous, with bri ht subnuclear foci in some cellsIn most or all cells for a short tem oral window eakin at the 100-200 cell sta eMan h odermal linea esMost h odermal linea esMost h odermal linea esMaternal, retained and strengthened in C and D lineages, later expressed in posterior hypodermis andmesodermal cellsMaternal, retained and strengthened in C and D lineages, later expressed in posterior hypodermis andmesodermal cellsEx ressed in all har n eal recursors and more weakl in intestinal and rectal recursorsEx ressed in ABa-derived neuronal and h odermal linea es, MS muscle, C h odermis and osteriorEx ressed after 350 cells in neurons includin AINL, ILshL, ASKL, ASKR, AIYL, AIYR,SMDDLEx ressed in a subset of D muscle, C h odermis, intestine, and AB -derived h odermis and neuronsEx ressed in a subset of AB -derived h odermis and neurons


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protein CND-1 DIE-1correlation coefficient 0.85 0.66

replication fraction 0.98 1

LIN-12/Notch nuclear GFP fluorescence compsister lineage pairs with/without known Notch

si nalinEel/Ear ABplpap/ABprpap

significance of the fluorescence intensitydifferences

2.87E-05 4.77E-05

Reproducibility between different types of repreporter types protein/protein promoter/promoter

reproducibility (mean correlation coefficient) 0.79 0.85re lication fraction 0.94 0.99number of strains 13 92

Reproducibility between independent strains f


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EGL-5 PAL-1 UNC-1300.8 0.89 0.91

0.98 1 0.93

red to sister lineages without signaling

ABplaaa/ABpraaa

0.036309133

rtersprotein/promoter

0.540.6212

r the same protein

nihms400638 supplement 03

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