nikon techinical setup c1 c1si setup screen expanded

175
CONFOCAL MICROSCOPE C1 (Spectral Imaging System C1si) <SETUP> This manual is intended to provide setup instructions for Nikon representatives who have attended the lecture on laser safety and have been trained in setup operations. Attempts by others to set up the product may lead to accidents or equipment failure. NIKON is not responsible for personal injury or equipment damage resulting from equipment setup performed by unqualified persons. Be sure to observe the warnings and cautions described in this manual to ensure safety during setup. M296E 05.5.NF.6 WARNING

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Nikon Confocal Microscope Technical Setup

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Page 1: Nikon Techinical Setup C1 C1si Setup Screen Expanded

CONFOCAL MICROSCOPE C1 (Spectral Imaging System C1si)

<SETUP>

This manual is intended to provide setup instructions for Nikon representatives who have attended the lecture on laser safety and have been trained in setup operations. Attempts by others to set up the product may lead to accidents or equipment failure. NIKON is not responsible for personal injury or equipment damage resulting from equipment setup performed by unqualified persons. Be sure to observe the warnings and cautions described in this manual to ensure safety during setup.

M296E 05.5.NF.6

WARNING

Page 2: Nikon Techinical Setup C1 C1si Setup Screen Expanded
Page 3: Nikon Techinical Setup C1 C1si Setup Screen Expanded

i

Introduction

This product is a laser apparatus that uses Class IIIB lasers (air-cooled Ar lasers, He-Ne lasers, semiconductor lasers). Accidental exposure of eyes or skin to laser light may result in injury or other problems. Confirm that there is no one in the area before turning on the lasers for adjustment. Always wear laser-protection goggles when using the laser.

This laser product was designed and manufactured in compliance with the "Performance Standards for Light-Emitting Products" stipulated by the FDA in the USA and "Safety of Laser Products" (IEC 60825-1) stipulated by the IEC. Compliance with FDA standards is in accordance with Laser Notice No. 50. Those using this laser product must complete laser safety courses and C1 setup training. • FDA Class IIIB laser product

• IEC Class 3B laser product

All manuals and accessories used in this laser product and provided by the distributor must be provided to the user.

Warning and Caution symbols in this manual

The following symbols are used in this manual to highlight important safety points. Carefully read and follow the instructions that accompany these symbols.

Symbol Meaning

Disregarding instructions accompanying this symbol may lead to serious injury or death.

Disregarding instructions accompanying this symbol may lead to injury or property damage.

All procedures should be performed safely and in accordance with the instructions in this manual to prevent accidents or equipment failures.

WARNING

WARNING

WARNING

WARNING

CAUTION

Page 4: Nikon Techinical Setup C1 C1si Setup Screen Expanded

Contents

ii

Contents

I System Configuration .................................................................................... 1

1 System ...................................................................................................................1 2 System Configuration ...............................................................................................3

II Laser Safety................................................................................................. 7

1 Laser Path................................................................................................................7 1.1 Laser Unit .......................................................................................................7 1.2 Scan Head.......................................................................................................8 1.3 Spectral Detector Unit.....................................................................................10 1.4 Standard Detector Unit ...................................................................................11 1.5 Upright Microscope Set ...................................................................................12 1.6 Inverted Microscope Set..................................................................................12

2 Safety Labels and Components with Apertures..............................................................13 2.1 Controller ......................................................................................................14 2.2 Eclipse 80i/90i, DIH, and Scan Head.................................................................15 2.3 Eclipse 80i/90i, U-Epi, and Scan Head...............................................................16 2.4 E800/E1000 and Scan Head ............................................................................17 2.5 E600 and Scan Head.......................................................................................18 2.6 TE2000 and Scan Head ...................................................................................19 2.7 Three-laser Unit EX ........................................................................................20 2.8 Three-laser Unit S and Three-laser Unit ............................................................21 2.9 Two-laser Unit S and Two-laser Unit..................................................................22 2.10 Two-detector Unit...........................................................................................23 2.11 Three-detector Unit ........................................................................................23 2.12 Spectral Detector Unit.....................................................................................24 2.13 Transmission Detector.....................................................................................25

III Preparations............................................................................................... 26

1 Checking Items and Tools........................................................................................26 1.1 Required Items ..............................................................................................26 1.2 Tools.............................................................................................................27

2 Overview of Setup Procedure...................................................................................28 IV Setting Up the PC and Operation Software...................................................... 30

1 Installing the AOM Control Board (Only When the AOM Unit Is Used.)...........................30 2 Network Settings ...................................................................................................30 3 Installing the Software............................................................................................32 4 System Settings.....................................................................................................32

4.1 AOM Control Board Setting: [Bleach] Tab ..........................................................32 4.2 Laser-type Setting: [Lasers] Tab ......................................................................34 4.3 Pinhole-break Delay: [Pinhole] Tab ...................................................................37 4.4 Controller IP Address Setting: [Information] Tab ................................................38 4.5 Channel Setting: [Grabber] Tab........................................................................39 4.6 Laser Power Meter Setting: [Power Monitor] Tab ................................................40 4.7 Mirror Options................................................................................................41

V Microscope................................................................................................. 42

1 Setting Up the Eclipse 80i/90i and the DIH................................................................43

Page 5: Nikon Techinical Setup C1 C1si Setup Screen Expanded

Contents

iii

2 Setting up the Eclipse 80i/90i, U-Epi, and Dedicated Trinocular Eyepiece Tube.............................43 3 Setting Up the E800/E1000 .....................................................................................44 4 Setting Up the E600/E600FN ...................................................................................45 5 Setting Up the TE2000-E/U .....................................................................................46 6 Attaching the Transmission Detector Unit ..................................................................51

6.1 Attaching the Transmission Detector Unit to the Microscope.................................52 6.2 Incorporating the Electric Board and the Light Guide Fiber into the C1 Controller ...58

6.2.1 For Transmission Detector (MHE47100)...................................................58 6.2.2 For Motorized Transmission Detector (MHE47200) ....................................61

VI Scan Head ................................................................................................. 65

1 Installing the Ring Adapter ......................................................................................65 2 Securing the Scan Head in Place ..............................................................................66

2.1 For the Eclipse 80i/90i ....................................................................................66 2.2 For the E800/E1000........................................................................................67 2.3 For the E600/E600FN......................................................................................68 2.4 For the TE2000-E/U........................................................................................69

3 Inserting the First Dichroic Mirror.............................................................................70 4 Connecting the Optical Fiber Cables..........................................................................71

VII Detector Unit.............................................................................................. 72

1 Spectral Detector Unit ..............................................................................................72 2 Standard Detector Unit.............................................................................................73

VIII Laser Unit.................................................................................................. 74

1 System Description..................................................................................................75 2 Two-laser Unit S, Two-laser Unit, Three-laser Unit S, and Three-laser Unit.....................77

2.1 Preparation....................................................................................................77 2.2 Laser Installation ...........................................................................................78 2.3 Laser Rough Adjustment .................................................................................80 2.4 Final Adjustment for the Ar Laser Light with the Optical Fiber ..............................87 2.5 Final Adjustment for the He-Ne Laser Light and 405 Laser Light with the Optical Fiber.....94 2.6 Attaching the Sheet Metal Covers.....................................................................96

3 Three-laser Unit EX ................................................................................................97 3.1 Preparation....................................................................................................98 3.2 Laser Installation ......................................................................................... 100 3.3 Laser Rough Adjustment 1............................................................................. 103 3.4 Laser Rough Adjustment 2............................................................................. 115 3.5 Final Adjustment for the Ar Laser Light with the Optical Fiber ............................ 122 3.6 Final Adjustment for the He-Ne Laser Light and 405 Laser Light with the Optical Fiber ...129 3.7 Adjusting the AOM Controller ......................................................................... 131 3.8 Attaching the Sheet Metal Cover .................................................................... 133

IX Controller .................................................................................................135

1 Installing the Second Line-grabber Board (MHE41900)................................................... 135 2 Connecting the Cable for the Interlock Unit (MHB47100) for Inverted Microscopes ....... 137

3 Connecting the DIH Interlock Cable (MXA22046) ..................................................... 137

4 AOM Unit SYNC Cable Connection........................................................................... 138

X Connecting Cables Between Units.................................................................139

XI General Adjustment ...................................................................................144

Page 6: Nikon Techinical Setup C1 C1si Setup Screen Expanded

Contents

iv

1 Confirmation of the Standard Detector Unit ............................................................. 144 2 Checking the TE2000 Interlock Unit........................................................................ 146 3 Checking the DIH Interlock.................................................................................... 147 4 Adjusting the Scan Head Mount ............................................................................. 148

4.1 Upright Microscope....................................................................................... 148 4.2 Inverted Microscope ..................................................................................... 154

5 Adjustment and Confirmation of the Fluorescent Image ............................................ 156 6 Compensating the Image Offset for the Bi-directional Scanning ................................. 157 7 Microscope Settings for the Diascopic DIC Microscopy............................................... 161 8 Accuracy Check of the Spectral Detector Unit.............................................................. 162

XII Confirmation and Check Sheet Entry.............................................................163

XIII Performance and Specifications....................................................................165

Page 7: Nikon Techinical Setup C1 C1si Setup Screen Expanded

1

System ConfigurationI

1 System (1) C1-SHV

(1) Monitor

(2) PC

(3) Keyboard

(4) Mouse

(5) Control Software

(6) Scan head

(7) First dichroic mirror

(8) Laser unit

(9) Detector unit

(10) Fluorescent filter

(11) Optical fiber for the excitation light

(12) Optical fiber for the fluorescence light

(13) Microscope

(14) Transmission detector (Cable connections are necessary only for the motorized transmission detector.)

(15) Controller

(16) AOM controller

Electrical connection

Optical connection

(1)

(5)

(4)

(2)

(6)

(7) (8)

(9)

(10)

(11)

(12)

(13) (14) (15)(16)

(3)

Page 8: Nikon Techinical Setup C1 C1si Setup Screen Expanded

I System Configuration

2

(2) C1-SHS

(1) Monitor

(2) PC

(3) Keyboard

(4) Mouse

(5) Control Software

(6) Scan head

(7) First dichroic mirror

(8) Laser unit

(9) Standard detector unit

(10) Spectral detector unit

(11) Fluorescent filter

(12) Optical fiber for the excitation light

(13) Optical fiber for the fluorescence light (For the standard detector)

(14) Optical fiber for the fluorescence light (For the spectral detector)

(15) Microscope

(16) Transmission detector (Cable connections are necessary only for the motorized transmission detector.)

(17) Controller

(18) AOM controller

(1)

(5)

(3) (4)

(2)

(6)

(7)

(8)

(9)

(10)

(11)

(17)

(18)

(12)

(13)

(14)

(15) (16)

Electrical connection

Optical connection

Page 9: Nikon Techinical Setup C1 C1si Setup Screen Expanded

I System Configuration

3

2 System Configuration (1) SHV

Unit Name of equipment Model Remark

1 C1 scan head (including the controller)

C1-SHV (*1)

C1 ring adaptor L C1-RAL 1

C1 ring adaptor S C1-RAS

Refer to “1 Installing the Ring Adapter” in Chapter VI “Scan Head” for details of application.

1 PC Commercially-available PC OS: Windows 2000 Professional with service pack 4 or higher or Windows XP Professional with service pack 2 or higher

CPU: Intel Pentium4 (3.2 GHz or higher processor recommended.)

RAM: 1 GB min.(recommended)

Network ports: 2 (One of the two is Intel 82550 compatible.)

Graphics card: True color mode (24-bit), SXGA or higher

Other: Keyboard, mouse, CD-ROM drive

Confocal Microscope C1

1 Network cable Commercially-available cable 100 Base-T (cross wired cable)

Software 1 EZ-C1 EZ-C1 Ver.3.0 or higher Contact your distributor for the latest versions.

C1 dichroic mirror 488/543/633 C1 1STDM 488/543/633

C1 dichroic mirror 488/594 C1 1STDM 488/594

C1 dichroic mirror 488/543 C1 1STDM 488/543

C1 dichroic mirror 408/488/543 C1 1STDM 408/488/543

First dichroic mirror

1

C1 dichroic mirror 408/514 C1 1STDM 408/514

Select the mirror suitable for the laser and use it with the fl-filter set described in Table 2.

C1 two-detector unit C1-DUD See Table 2 to find the appropriate fl-filter.

Detector unit 1

C1 three-detector unit C1-DUT The detector board (C1-CU-EDB) is required for use with this model.

See Table 2 to find the appropriate fl-filter.

*1 This product is not compatible with the previous set. Be careful.

Page 10: Nikon Techinical Setup C1 C1si Setup Screen Expanded

I System Configuration

4

(2) SHS

Unit Name of equipment Model Remark

1 C1 scan head (including the controller and spectral detector)

C1-SHS (*2) The controller and spectral detector are included in the C1-SHS.

C1 ring adaptor L C1-RAL 1

C1 ring adaptor S C1-RAS

Refer to “1 Installing the Ring Adapter” in Chapter VI “Scan Head” for details of application.

1 PC Commercially-available PC OS: Windows 2000 Professional with service pack 4 or higher or Windows XP Professional with service pack 2 or higher

CPU: Intel Pentium4 (3.2 GHz or higher processor recommended.)

RAM: 1 GB min.(recommended)

Network ports: 2 (One of the two is Intel 82550 compatible.)

Graphics card: True color mode (24-bit), SXGA or higher

Other: Keyboard, mouse, CD-ROM drive

Confocal Microscope C1

1 Network cable Commercially-available cable 100 Base-T (cross wired cable)

Software 1 EZ-C1 EZ-C1 Ver.3.0 or higher Contact your distributor for the latest versions.

C1 dichroic mirror BS 20/80 C1 1STDM BS 20/80 Used for the spectral detector.

C1 dichroic mirror 488/543/633 C1 1STDM 488/543/633

C1 dichroic mirror 488/594 C1 1STDM 488/594

C1 dichroic mirror 488/543 C1 1STDM 488/543

C1 dichroic mirror 408/488/543 C1 1STDM 408/488/543

First dichroic mirror

1

C1 dichroic mirror 408/514 C1 1STDM 408/514

Select the mirror suitable for the laser and use it with the fl-filter set described in Table 2.

Spectral Detector unit

1 C1 spectral detector unit C1-SHS The scan head and controller are included in the C1-SHS.

C1 two-detector unit C1-DUD See Table 2 to find the appropriate fl-filter.

Standard Detector unit

1

C1 three-detector unit C1-DUT The detector board (C1-CU-EDB) is required for use with this model.

See Table 2 to find the appropriate fl-filter.

*2 This product is not compatible with C1-SHV. Be careful.

(3) Common units

Unit Name of equipment Model Remark

C1 two-laser unit S or

two-laser unit

C1-LU2S (*3)

C-LU2 (*3)

See Table 1 for mountable lasers.

C1 three-laser unit S or

three-laser unit

C1-LU3S (*3)

C-LU3 (*3)

See Table 1 for mountable lasers.

Laser unit 1

C1 three-laser unit EX or

three-laser unit EX

C1-LU3EX (*3)

C-LU3EX (*3)

See Table 1 for mountable lasers.

Attachment: • Ar wavelength switch board

(C1-EX) • AOM unit (C1-AOM)

Page 11: Nikon Techinical Setup C1 C1si Setup Screen Expanded

I System Configuration

5

Unit Name of equipment Model Remark

Eclipse 80i/90i 80i/90i The DIH interlock cable (C1-DIH) and C1 TE2 mount (C1-TE) are necessary to connect with the DIH.

Use the C1E6 trinocular eyepiece tube (C1-Y-TT) if not using the DIH.

E1000/E800 E1000/E800 The C1E8 beam-splitter module (C1-E8) is required for use with this model.

E600 E600 The C1E6 trinocular eyepiece tube (C1-Y-TT) is required for use with this model.

Microscope 1

TE2000E/U TE2000E/TE2000U All of the following equipment is required for use with this model:

• C1 TE2 shutter (C1-T-SM) • C1 TE2 mount (C1-TE) • C1 TE2 light shield (C1-T-SP-U)

C1 transmission detector C1-TD The detector board (C1-CU-EDB) is required for use with this model.

(1)

C1 Motorized transmission detector

C1-ETD The detector board (C1-CU-EDB) is required for use with this model. (*4)

C1 transmit adapter U C1-TD-U This adapter is required for the E1000, E800, and E600.

C1 transmit adapter I C1-TD-I This adapter is required for the TE2000.

Transmission detector unit (optional)

(1)

C1 transmit adapter J C1-TD-J This adapter is required for the Eclipse 80i/90i.

Z focus unit E6 For E600 only

Z focus unit E8 For E800 only

Z focus unit TE2 For TE2000U only

Z focus (optional)

(1)

Z focus unit C

Remote focus accessory

For all units

In this manual, E600 represents the following five models, E600, E600 POL, E600W, E600W POL, and E600FN. *3 This product is not compatible with the previous set. Be careful. *4 This product cannot be mounted on E1000, E800, and E600. Be careful.

Table 1 Wavelength Product Specifications Mountable laser unit (position)

Manufacturer: Coherent

Head: RADIUS 405

Power supply: CDRH control box

405±5nm/25mW The 408 laser adapter R (C1-LAR) is required for use with the following units:

C1-LU3S (L1), C-LU3 (L1)

C1-LU3EX (L1), C-LU3EX (L1)

Manufacturer: Melles Griot

Head: 56 ICS 423 (*5)

Power supply: 56 IMA 016

408±5nm/17mW The 408 laser adapter S (C1-LAS) is required for use with the following units:

C1-LU3EX (L1), C-LU3EX (L1)

Violet

Manufacturer: Melles Griot

Head: 56 ICS 426 (*5)

Power supply: 56 IMA 016

440 to 445 nm/ 15 mW

The 408 laser adapter S (C1-LAS) is required for use with the following units:

C1-LU3EX (L1), C-LU3EX (L1)

Page 12: Nikon Techinical Setup C1 C1si Setup Screen Expanded

I System Configuration

6

Wavelength Product Specifications Mountable laser unit (position)

Manufacturer: Spectra-physics

Head: 163-C02

Power supply: 263-C04

488 and 514 nm/ 40 mW

C1-LU3EX (L2), C-LU3EX (L2)

Manufacturer: Spectra-physics

Head: 163-C12

Power supply: 263-C04

488nm/25mW C1-LU3S (L2), C-LU3 (L2)

C1-LU3EX (L2), C-LU3EX (L2)

Manufacturer: Spectra-physics

Head: 161C-030

Power supply: 263-C04

488nm/10mW C1-LU2S (L1), C-LU2 (L1)

C1-LU3S (L2), C-LU3 (L2)

C1-LU3EX (L2), C-LU3EX (L2)

Manufacturer: Melles Griot

Head: IMA101040

Power supply: Model 175B

488 and 514 nm/ 40 mW

C1-LU3EX (L2), C-LU3EX (L2)

Blue

Manufacturer: Coherent

Head: Sapphire 488-20

Power supply: CDRH controller

488nm/20mW The 488 laser adapter (C-LAR) is required for use with the following units:

C1-LU3EX (L2), C-LU3EX (L2)

Green Manufacturer: Melles Griot

Head: 25LGP 193

Power supply: 05LPL951-065/100V, 05LPL915-065/115-230V

543nm/1mW C1-LU2S (L2), C-LU2 (L2)

C1-LU3S (L3), C-LU3 (L3)

C1-LU3EX (L3), C-LU3EX (L3)

Manufacturer: Melles Griot

Head: 85YCA010

Power supply: 85YCA series control box

561 nm/10 mW A laser adapter is required for use. For the adapter information, contact your nearest Nikon representative. C1-LU3EX (L3), C-LU3EX (L3)

Yellow

Manufacturer: JDS Uniphase

Head: 1677P

Power supply: 1207-1/115V, 1207-2/230V, 1207-3/100V

594nm/2mW C1-LU2S (L2), C-LU2 (L2)

C1-LU3S (L1), C-LU3 (L1)

C1-LU3EX (L1), C-LU3EX (L1)

Manufacturer: JDS Uniphase

Head: 1125P

Power supply: 1202-3/100V, 1202-1/115V, 1202-2/230V

633nm/5mW C1-LU3S (L1), C-LU3 (L1)

C1-LU3EX (L1), C-LU3EX (L1)

Manufacturer: Melles Griot

Head: 56 ICS 272 (*5)

Power supply: 56 IMA 015

635nm/3mW The 408 laser adapter S (C1-LAS) is required for use with the following unit:

C1-LU3EX (L1), C-LU3EX (L1)

Red

Manufacturer: Melles Griot

Head: 56 ICS 264 (*5)

Power supply: 56 IMA 015

640nm/12mW The 408 laser adapter S (C1-LAS) is required for use with the following unit:

C1-LU3EX (L1), C-LU3EX (L1)

*5 To use the 56ICS series laser manufactured by Melles Griot, select a Nikon specification type laser that has the base plate of 6 mm thickness and a cable for the modulation.

Table 2

Type Name of equipment Performance Application

C1 fl-filter 488/594 HQ535/50m, 570dclp, HQ630LP

C1 fl-filter 488/543 HQ515/30m, 540dclp, HQ570LP

Fl 2 1

C1 fl-filter 408/514 HQ480/25, 505dclp, HQ540/30

The fl-filter 2 for the detector unit 2 is compatible with the detector unit 3. Use the appropriate filter with the same wavelength as that of the first dichroic mirror.

C1 fl-filter 488/543/633-1

C1 fl-filter 488/543/633-2

HQ515/30m, 540dclp, HQ590/50m, 625dclp, HQ650LP

Fl 3 1

C1 fl-filter 408/488/543-1

C1 fl-filter 408/488/543-2

HQ450/35m, 480dclp, HQ515/30m, 545dclp, HQ605LP/75m

The fl-filter 3 is used only for the detector unit 3.

Use the appropriate filter with the same wavelength as that of the first dichroic mirror.

Page 13: Nikon Techinical Setup C1 C1si Setup Screen Expanded

2005/05/23

7

Laser SafetyII

1 Laser Path

1.1 Laser Unit

Fig II-1

(1) L1 (See the table below.)

(2) L2 (See the table below.)

(3) L3 (See the table below.)

(4) Motorized shutter

(5) ND filter (ND4, ND8)

(6) Laser synthesis mirror

(7) Laser outlet (single-mode fiber)

(8) Continuous light control (optional AOM)

(9) Wavelength selection filter

Table II-1

L1 L2 L3

Two-laser unit S and two-laser unit

Ar laser (single line) He-Ne laser (green or yellow)

Three-laser unit S and three-laser unit

408 laser, Red or yellow laser Ar laser (single line) He-Ne laser (green)

Three-laser unit EX 408 laser, red or yellow laser,440 laser

Ar laser (single line or multiple lines) or 488 solid-state laser

He-Ne laser (green) 561 solid-state laser

(2)

(1)

(8)

(7)

(4)

(3)

Three-laser unit EX (C1-LU3EX, C-LU3EX)

Three-laser unit S(C1-LU3S)

three-laser unit (C-LU3)

Two-laser unit S (C1-LU2S)

two-laser unit (C-LU2)

(6)

(9)

(2)

(3)

(4) (5)

(6)

(4)(5)

(6) (7) (7)

(1)

(2)

laser optical path

(1)

Page 14: Nikon Techinical Setup C1 C1si Setup Screen Expanded

II Laser Safety

8

1.2 Scan Head (1) C1-SHV

(1) Laser inlet (single-mode fiber)

(2) Collimator lens

(3) First dichroic mirror

(4) Scanner mirror

(5) Scanner lens

(6) Collector lens

(7) Pinhole

(8) Fluorescence outlet (high-efficiency multi-mode fiber)

(3)

(5)(7)

(8)

(1)

(2)

(4)

(6)

Page 15: Nikon Techinical Setup C1 C1si Setup Screen Expanded

II Laser Safety

9

(2) C1-SHS

Fig II-2

(1) Laser inlet (single-mode fiber)

(2) Collimator lens

(3) First dichroic mirror

(4) Scanner mirror

(5) Scanner lens

(6) Collector lens

(7) Pinhole

(8) Fluorescence outlet to the standard detector (high-efficiency multi-mode fiber)

(9) Fluorescence outlet to the spectral detector (high-efficiency multi-mode fiber)

(3)

(5)(7)

(8)

(1)

(2)(4)

(6)

(6)

(6)

(9)

Page 16: Nikon Techinical Setup C1 C1si Setup Screen Expanded

II Laser Safety

10

1.3 Spectral Detector Unit

[Optical path detail]

(1) Fluorescence inlet into the spectral detector (high-efficiency multi-mode fiber)

(2) Collimator lens

(3) Polarized beam splitter

(4) Total reflection mirror

(5) Fresnel rhomb wave plate

(6) Diffraction grating

(7) Concave mirror

(8) Optical detector array

(1)

(8)

(7)

(2) (4) (5) (6)

(8)

(6)

(7)

(7)

(4)

(1)

(2) (3)

(5)

Page 17: Nikon Techinical Setup C1 C1si Setup Screen Expanded

II Laser Safety

11

1.4 Standard Detector Unit

Fig II-3

(1) Fluorescence inlet into the standard detector (high-efficiency multi-mode fiber)

(2) Collector lens

(3) First fluorescent filter block

(4) Second fluorescent filter block

(5) Optical detector (channel 1)

(6) Optical detector (channel 2)

(7) Optical detector (channel 3)

(5)

(1)

(2)

(6) (3) (6) (3) (4)

(1)

(2)

(7) (5)

Two-detector unit Three-detector unit

Page 18: Nikon Techinical Setup C1 C1si Setup Screen Expanded

II Laser Safety

12

1.5 Upright Microscope Set

Fig II-4

1.6 Inverted Microscope Set

Fig II-5

Scan head Laser inletLaser unit Detector unit

Controller

Laser beam Optical fiber

Transmission detector

Transmission detector

Optical fiber

Laser beam

Controller

Page 19: Nikon Techinical Setup C1 C1si Setup Screen Expanded

II Laser Safety

13

2 Safety Labels and Components with Apertures

The following figures show the types and positions of safety labels on the various units, including components with apertures. The microscope labels are found in the C1 main unit package (scan head, controller). Affix these to the specified positions when setting up the C1. All other labels are attached before shipment.

(1) IEC warning label - Laser hazard symbol

(2) IEC explanatory label

(3) Safety standard label

(4) IEC aperture label

(5) IEC caution label - Class 3B areas

(6) IEC caution label - Class 3R areas

(7) IEC caution label - Class 3B areas (for laser unit)

Page 20: Nikon Techinical Setup C1 C1si Setup Screen Expanded

II Laser Safety

14

2.1 Controller

Check that the labels are affixed.

(1) IEC warning label - Laser hazard symbol

(2) IEC explanatory label

(3) Safety standard label

(1) (2)

(3)

Page 21: Nikon Techinical Setup C1 C1si Setup Screen Expanded

II Laser Safety

15

2.2 Eclipse 80i/90i, DIH, and Scan Head

The labels must be affixed to the positions shown in the diagram below.

(1) IEC warning label - Laser hazard symbol

(4) IEC aperture label

(5) IEC caution label - Class 3B areas

(6) IEC caution label - Class 3R areas

A Component with aperture

B Component with aperture

C Component with aperture

D Component with aperture

E Head unit

(5) (5) (5)

ApertureAperture

Aperture

A

B

C

D

(4)

(5)

(1) (5)

E

(5)

Aperture

(6)

Page 22: Nikon Techinical Setup C1 C1si Setup Screen Expanded

II Laser Safety

16

2.3 Eclipse 80i/90i, U-Epi, and Scan Head

The labels must be affixed to the positions shown in the diagram below.

(1) IEC warning label - Laser hazard symbol

(4) IEC aperture label

(5) IEC caution label - Class 3B areas

(6) IEC caution label - Class 3R areas

A Component with aperture

B Component with aperture

C Component with aperture

D Component with aperture

E Component with aperture

F Head unit

(5)

Aperture Aperture

Aperture

ApertureAperture

(5)

(5)

(5)

(5)

A

C D

E

(4)

(5)

(1)(5)

B

F

(6)

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2.4 E800/E1000 and Scan Head

The labels must be affixed to the positions shown in the diagram below.

(1) IEC warning label - Laser hazard symbol

(4) IEC aperture label

(5) IEC caution label - Class 3B areas

(6) IEC caution label - Class 3R areas

A Component with aperture

B Component with aperture

C Component with aperture

D Component with aperture

E Head unit

(5) (5)

(5)

Aperture

Aperture

Aperture

A

B

C

D

(4)

(5)

(1)

(5)

E

(5)

Aperture

(6)

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2.5 E600 and Scan Head

The labels must be affixed to the positions shown in the diagram below.

(1) IEC warning label - Laser hazard symbol

(4) IEC aperture label

(5) IEC caution label - Class 3B areas

(6) IEC caution label - Class 3R areas

A Component with aperture

B Component with aperture

C Component with aperture

D Component with aperture

E Component with aperture

F Head unit

(5)

Aperture

(5)

(5)

Aperture ApertureAperture

(5)

(5)

Aperture

A

C

D

E

(4)

(5)

(1)

(5)

B

F

(6)

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2.6 TE2000 and Scan Head

The labels must be affixed to the positions shown in the diagram below.

(1) IEC warning label - Laser hazard symbol

(4) IEC aperture label

(5) IEC caution label - Class 3B areas

(6) IEC caution label - Class 3R areas

A Component with aperture

B Component with aperture

C Component with aperture

C

(5)

Aperture

(5)

Aperture

(5)

Aperture

B A

(5)

(5)

(4)

(6) (1)

(5)

(5)

(5)

(5)

Aperture

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2.7 Three-laser Unit EX

Check that the labels are affixed.

(1) IEC warning label - Laser hazard symbol

(7) IEC caution label - Class 3B areas

(7)

(1)

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2.8 Three-laser Unit S and Three-laser Unit

Check that the labels are affixed.

(1) IEC warning label - Laser hazard symbol

(7) IEC caution label - Class 3B areas

(7)

(1)

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2.9 Two-laser Unit S and Two-laser Unit

Check that the labels are affixed.

(1) IEC warning label - Laser hazard symbol

(7) IEC caution label - Class 3B areas

(7)

(1)

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2.10 Two-detector Unit

Confirm that the label is affixed.

(6) IEC caution label - Class 3R areas

2.11 Three-detector Unit

Confirm that the label is affixed.

(6) IEC caution label - Class 3R areas

(6)

(6)

(6)

(6)

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2.12 Spectral Detector Unit

Confirm that the label is affixed.

(6) IEC caution label - Class 3R areas

View without the top cover

Only service personnel are allowed to open the cover.

Internal cover

Aperture (top view without the internal cover)

(6)

Top view (view A)

WARNING

A

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2.13 Transmission Detector

Confirm that the label is affixed.

(5) IEC caution label - Class 3B areas

(5)

(5)

[C1-TD: Manual operation type]

[C1-ETD: Motorized switch type](5)

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PreparationsIII

1 Checking Items and Tools

1.1 Required Items

Make sure that you have all of the following items before starting.

• PC system (main unit, monitor, mouse, keyboard, network board, 100Base-T cross wired cable) (See “2. System Configuration” in “I. System Configuration.”)

• PC accessories (network board, network cable, PCI-6711 board [for optional AOM unit]) (See “2. Sytem Configuration” in “I. System Configuration.”)

• Operating system (See “2. System Configuration” in “I. System Configuration.”)

• Controller: Power cable

• Laser: Recommended lasers (See “2. System Configuration” in “I. System Configuration.”)

• Fluorescent filter

• Focus motor: Optional

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1.2 Tools

Before starting, make sure that you have the following tools required to set up the C1 system.

General tools

• Allen wrench (mm, inch) • Phillips screwdriver

• Fluorescent slide sample (you can also use a HE-stained sample)

Special tools

• Laser-protection goggles

• Calibrated power meter

• Power measurement attachment

• Dichroic mirror-centering tool

• Coupler-centering tool

• Centering tool for pupil observation and the mirror for observing the centering tool

• Nikon 2-piece M4 screwdriver set (with red handles)

• Polarization orientation adjustment tool and the polarizer-equipped tool

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2 Overview of Setup Procedure

1 PC assembly and software installation

(Chapter IV)

・ PCI-6711 board installation and driver software installation (only

when using the AOM unit)

・ Network card installation and network setup

・ Software (EZ-C1) installation

・ System setup

2 Microscope setup (Chapter V)

・ Microscope setup

・ Interlock unit installation (only when using the TE2000E/U)

・ Transmission detector installation (only when using the

transmission detector)

3 Scan head setup (Chapter VI)

・ Ring adapter installation

・ Installation onto the microscope

・ First dichroic mirror installation

・ Optical fiber connection

4 Detector unit setup (Chapter VII)

・ Spectral detector unit

* Check the environmental conditions of the installation location. The spectral detector unit must be installed under 35 ºC.

・ Standard detector unit

5 Laser unit assembly and adjustment

(Chapter VIII)

・ For two-laser unit S , two-laser unit, three-laser unit S, or

three-laser unit

・ For three-laser unit EX

* To use a laser diode or a solid-state laser with the spectral detector unit, its wavelength must be measured with a spectrometer and registered into the EZ-C1 beforehand.

* You must have performed the following Controller setup procedure when using an AOM unit.

6 Controller setup (Chapter IX)

・ Board installation (only when using the three-detector unit or

transmission detector)

・ Interlock unit cable connection (when using the Eclipse 80i/90i and

DIH, and when using the TE2000E/U)

・ AOM unit SYNC cable connection (only when using the AOM unit)

7 Unit cable connections (Chapter X)

・ Controller – laser unit cable connection

・ Controller – scan head cable connection

・ Controller – detector unit cable connection

・ Controller – PC cable connection

・ Controller – microscope connection (when using the transmission

detector, when using the Eclipse 80i/90i and DIH, and using the TE2000E/U)

・ AOM unit cable connection (only when using the AOM unit)

・ PC – microscope connection (only when using the TE2000E)

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8 General adjustment (Chapter XI)

・ Detector unit check

・ Interlock unit operation check (when using the TE2000E/U and

when using the Eclipse 80i/90i and DIH)

・ Shading (brightness uniformity adjustment)

・ Adjustment and check for the fluorescent image

・ Microscope setup for diascopic DIC microscopy (only when using

the transmission detector)

・ Accuracy check of the spectral detector unit

9 Confirmation and check sheet entry

(Chapter XII)

・ Laser safety check

・ Check sheet entry

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Setting Up the PC and Operation Software IV

1 Installing the AOM Control Board (Only When the AOM Unit Is Used.)

The following procedure describes how to install the PCI expansion board (manufactured by National Instruments) in the PC. This board is used to control the AOM unit.

(1) Install the control board for controlling the AOM unit in a free PCI slot in the PC.

(2) Install the control board driver software using the driver CD-ROM (provided).

2 Network Settings

Establish network settings for one of the two network cards installed in the PC for direct connection of the cross wired cable to the C1 controller.

(1) Right-click on the [My Network] icon on the desktop and select [Properties] from the pop-up menu.

The [Network and Dial-up Connections] window opens.

(2) Check the device name to confirm that the network card is connected to the C1 controller. Right-click the card name and select [Properties] from the pop-up menu.

The [Local Area Connection Properties] window opens.

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(3) Select “Internet Protocol (TCP/IP)” and click on the [Properties] button.

The “Internet Protocol (TCP/IP) Properties” window opens.

(4) Check the “use the following IP address:”, and set the IP address and subnet mask as follows:

IP address ・・・ 192.168.255.253

Subnet mask ・・・ 255.255.255.248

* The procedure for displaying the network settings window will differ somewhat on PCs running Windows XP.

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3 Installing the Software

The Installation Wizard will launch when you insert the “Install” CD-ROM in the CD-ROM drive. Follow the instructions displayed on screen to install the software.

Installing this version of EZ-C1 (Ver 3.00) on a PC on which EZ-C1 Ver 2.30 is already installed will automatically uninstall Ver 2.30. For more information, refer to the EZ-C1 Software Manual.

4 System Settings

Launch EZ-C1 and select [Configure] [Confocal C1] from the menu to display the screen shown below. Select the required tabs and enter the corresponding system settings.

4.1 AOM Control Board Setting: [Bleach] Tab

Set so that EZ-C1 can access the AOM unit control board installed in the PC.

(1) Press the [Advanced…] button.

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(2) Confirm that the [Device Number] in the setting window is <1>. Set [External Reference Voltage] to <2.500 V>. Press the OK button to close the window.

* Do not alter any of the other settings.

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4.2 Laser-type Setting: [Lasers] Tab

The name, wavelength, and AOM module must be set for the laser used with the EZ-C1.

・To set the AOM module

(1) Enter a suitable name for the laser in the [Name] field. Select the laser wavelength from the [Wavelength] pull-down menu.

(2) Select control channel 1 or 2 from the [Laser Control] pull-down menu for each laser to control AOM units.

(3) Set [Brand:] for [Device Type] in the following setup dialog window. Select the device to be controlled using the control board (manufactured by National Instruments) and set the control voltage. To set the AOM device, select <Nikon AOM> from the pull-down menu.

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(4) In the following Select Calibration Table dialog, select the wavelength data for the laser on which the AOM is installed.

(5) Enter the AOM Setting default value when starting EZ-C1 in [Initial Value:]. Select <100%> from the [Laser Power:] pull-down menu under [Calibration]. Set [Logarithmic Scroll:] under [Calibration] to <1.0>.

* These procedures must be repeated for all lasers with AOMs installed.

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・408 laser diode settings

(1) Select control channel <4> from the [Laser Control] pull-down menu for the corresponding laser to control a 408 laser diode.

(2) Select <Melles Griot> from the [Brand:] pull-down menu under [Device Type] in the following setup dialog window.

(3) Select the name <56ICS423 408.cal> in the following Select Calibration Table dialog window.

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4.3 Pinhole-break Delay: [Pinhole] Tab

The timing with which the pinhole (selected in the [Selection] field) is click-stopped can be adjusted in the [Break Delay] field. (This procedure is usually unnecessary.) The default setting for [Mode] in the [Break Delay] field is <initial setting>, meaning that the value preset in the controller will be used. Changing the [Mode] to <manual setting> allows the value to be set manually in the field below. Adjust the delay time accordingly when the movement of the pinhole is not determined by the value of the <initial setting>. The controller must be restarted to restore the <initial setting> after changing to <manual setting>.

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4.4 Controller IP Address Setting: [Information] Tab

The procedure below describes how to modify IP addresses. When the software runs for the first time, the window will display the default IP address (this is not shown in the screen shown below). Check the indicated IP address. If it differs from the address given below, correct the setting.

Controller IP address: 192.168.255.254

(1) Disconnect the network cable linking the C1 controller to the PC.

(2) Launch the EZ-C1.

A C1 controller connection timeout error occurs.

(3) Press the Cancel button to display the IP address setup window.

(4) Pressing the Default button here sets <192.168.255.254> as the C1 controller IP address.

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4.5 Channel Setting: [Grabber] Tab

When two line-grabber boards are used for three input channels, the following setting must be made so that the PMT-gain setting and the input image display can be controlled using the software. For this purpose, input “3” for [Number] in the [Channel] field.

When the transmission detector is installed on the fourth channel, set “4” for [Number] and check the [4] checkbox in the [Enable] field.

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4.6 Laser Power Meter Setting: [Power Monitor] Tab

Enter the following setting in the window below to monitor the laser power.

* The settings entered in this window will be used only for the laser power monitoring displayed in the [Laser Power Monitor] toolbar. Only certain settings are used in the power monitoring for each laser displayed in the [Laser Control Bar] toolbar.

The setting details are as follows:

• [Measure Total Power each] Specifies the interval for monitoring laser power.

• [Measure Total Power each start:] Measures laser power when scanning starts and makes a note of it.

• [Time for the shutter to open:] Sets the wait time before the laser shutter opens.

• [Number of readings to average:] Specifies the number of laser power readings obtained and displayed as an average.

• [Lower Limit:] Sets the threshold at which <Under> is displayed on the laser power meter display.

• [Higher Limit:] Sets the threshold at which <Over> is displayed on the laser power meter display.

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4.7 Mirror Options

Specifying the options to change the scanning direction allows the obtained image to be flipped horizontally or vertically. Set the two options below to give an image matching the one seen through the binocular eyepiece.

The settings are as follows.

• [mirror image][x direction] Specifies the horizontal scanning direction.

• [mirror image][y direction] Specifies the vertical scanning direction.

* Do not change any of the other settings. For information on the various settings, refer to the EZ-C1 Software Manual.

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Microscope V

This section describes the preparations involved in attaching the scan head to the microscope. Compatible microscopes and mounting sections are listed below.

• Do not attempt to attach to microscopes other than those listed below.

Doing so may result in accidental exposure to laser radiation through the eyepiece.

• Turn off power supplies to all lasers and units before starting the setup procedures described here.

• Upright microscope: Eclipse 80i/90i, E1000, E800, E600, E600FN

• Inverted microscope: TE2000E, TE2000U

Microscope Eyepiece tube Mounting section

Fluorescent device

Ring Adaptor

DIH (using C1-TE) Front port Built-in L Eclipse 80i/90i Dedicated trinocular

eyepiece tube Camera port U-Epi L

E1000

E800

Dedicated beam splitter Rear port Built-in None

E600 Dedicated trinocular eyepiece tube

Camera port U-Epi L

Upright microscope

E600FN Dedicated trinocular eyepiece tube

Camera port U-Epi S

TE2000E Inverted microscope TE2000U

Provided (using C1-TE) Left side port Built-in S

WARNING

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1 Setting Up the Eclipse 80i/90i and the DIH

(1) Set up the Eclipse 80i/90i and the DIH.

(2) Connect the DIH to the controller using the interlock cable.

(3) Assemble the interlock unit cable (I-lock cable) inside the controller. Then, insert the

pin jack into the connector for the I-lock on the rear panel of the controller. For more information, see “3. Connecting the DIH Interlock Cable (MXA22046)” in “IX. Controller.”

(4) Affix the label provided with the C1 on the microscope, as shown in the diagram in "2.2 Eclipse 80i/90i, DIH and Scan Head" in "II. Laser Safety" (P.15).

2 Setting up the Eclipse 80i/90i, U-Epi, and Dedicated Trinocular Eyepiece Tube

(1) Set up the Eclipse 80i/90i, U-Epi, and dedicated trinocular eyepiece tube.

(2) Affix the label provided with the C1 onto the microscope, as shown in the diagram in "2.3 Eclipse 80i/90i, U-Epi, and Scan Head" in "II. Laser Safety" (P.16).

DIH interlock connector Rear of the DIH

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3 Setting Up the E800/E1000

(1) Set up the main E800/E1000 microscope unit.

(2) Mount the laser-safety C1-E8 beam-splitter module (MHD46110) on top of the microscope stand, and secure the module in place by following the same mounting procedure as for an ordinary beam-splitter module. (Unlike ordinary beam-splitter modules (such as the MAD56111), the C1-E8 module is equipped with a tilting mechanism on the vertical tube and features a different optical system.)

(3) Affix the label provided with the C1 onto the microscope, as shown in the diagram in "2.4 E800/1000 and Scan Head" in "II. Laser Safety" (P.17).

Fig. V-1

Fig. V-2

C1-E8 beam-splitter module (MHD46110)

Scan head mounting part

Scan head mounting part

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4 Setting Up the E600/E600FN

(1) Set up the main microscope unit.

(2) Mount the laser-safety trinocular eyepiece tube (MHB42200). Make sure that the notch (index mark) on the straight tube is positioned on the right side. Then tighten set screws A (3 locations).

(3) Affix the label provided with the C1 onto the microscope, as shown in the diagram in "2.5 E600 and Scan Head" in "II. Laser Safety" (P.18).

Fig. V-3

Trinocular eyepiece tube (MHB42200)

Straight tubeNotch(Index mark)

Set screw A

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5 Setting Up the TE2000-E/U

Install the interlock unit for added safety when using the inverted microscope. This unit (MHB47100) consists of a shutter module and illumination pillar switch. Attach the shutter module between the main microscope unit and eyepiece tube, and mount the illumination pillar switch on the pillar.

(1) Set up the main microscope unit.

(2) Affix the label provided with the C1 on the microscope, as shown in the diagram in "2.6 TE2000 and Scan Head" in "II. Laser Safety" (P.19).

(3) Attach the straight tube (TE mount) for the TE2000 to the side port. As shown in Fig. V-4, align the origin index mark on the straight tube with the reference index mark and attach the straight tube to the side port, making sure that the notch (index mark) faces the front side. Then tighten upper set screws B (2 locations).

Fig. V-4

Set screw B

Reference index mark

Origin index mark

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(4) Install the shutter module between the eyepiece tube and main microscope unit.

1) As shown in Fig. V-5, hook the shutter module on the two holes located on the bottom surface of the eyepiece tube.

Fig. V-5

2) Mount the shutter module and eyepiece tube to the TE2000 While supporting the bottom side of the shutter module with your hand to prevent it from disengaging from the holes, install the assembly by sliding completely into the square groove on the TE2000 main unit. Then tighten the set screw (one location) on the right side of the mount for the eyepiece tube (see Fig. V-6).

Precaution when attaching the shutter module to the TE2000

• When mounting the shutter module to the TE2000, be sure to turn the

module dial to the “Confocal” side (see Fig. V-7). If the shutter module is attached with the dial in the “Bino” position, the shutter module may be damaged.

• When inserting the assembly to the TE2000, support the shutter module with your hand to keep it from disengaging from eyepiece tube.

Shutter module

Hook the shutter module on these holes

CAUTION

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Fig. V-6

Fig. V-7

Precautions when removing the shutter module from the TE2000

• When unmounting the shutter module from the TE2000, be sure to turn

the dial to the “Confocal” side and lift the eyepiece tube and shutter module TOGETHER. The shutter module cannot be removed with the dial in the “Bino” position. Attempting to do so may damage the shutter module.

(2)Tighten the set screw

(1)

(2)

(1) Install the shutter module and eyepiece tube assembly by sliding into the square groove on the TE2000 main unit.

Turn the dial to the “Confocal” side.

CAUTION

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(5) Attach the illumination pillar switch (consisting of the switch box and angle piece) to the TE2000 100W illumination pillar.

1) As shown in Fig. V-8, attach the switch box to the illumination pillar mounting section and the angle piece (movable section) to the tilting section. Attach the angle piece with the tilting section tilted. Use the manipulator mounting tap holes (2 locations) to secure both.

Fig. V-8

2) Remove the upper cover of the switch box (Fig. V-9). Raise the illumination pillar to the original vertical position. In this state, make sure that the protrusion on the angle piece is pressing against (i.e., turning ON) the two microswitches (switch body) inside the switch box. If the protrusion does not press against the microswitches, or if it presses too hard on the switch body, loosen the screw (#209) and adjust the position of the switch body.

Fig. V-9

3) Install the upper cover on the switch box.

Screw (#209)

Microswitch

Make sure that the protrusion on the angle piece is pressing against the two microswitches.

Microswitch

Switch box with upper cover removed

Incline the illumination pillar tilting section

Angle piece

Switch box

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(6) Bind the cable from shutter module with the cable of the illumination pillar switch. Pass the cable from the shutter module through the cable catch located on the left side of the TE2000, as shown in Fig. V-10, and bind it with the cable of the illumination pillar switch. Then pass both cables through the cable catch located at the back of the illumination pillar to secure it in place.

* With the TE2000-E, the shutter module cable and illumination pillar switch cable can

be routed through the hole in the rear hub for secure installation.

Fig. V-10

(7) Insert the pin jacks into the I-Lock connectors on the controller rear panel only after inserting the I-Lock cable into the controller. For more information, see “2. Connecting the Cable for the Interlock Unit (MHB47100) for Inverted Microscopes” in “IX. Controller.”

Rear cable catch

Pass the cables through the cable catches.

Side cable catch

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6 Attaching the Transmission Detector Unit

• Before beginning the setup process, be sure to check that the lasers,

the controller, and the microscope are turned OFF. Note that the labels attached to the transmission detector unit warns of potential danger of exposing the eyes and skin to the laser beam if any laser is left ON during setup. (See “2.13. Transmission Detector” in “II. Laser Safety.”)

Fig. V-11

WARNING

Mounting to the upright microscope Mounting to the inverted microscope

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6.1 Attaching the Transmission Detector Unit to the Microscope

I C1-TD (manual operation type)

When using the Eclipse 80i, E800, E1000, or E600/E600FN

(1) Detach the diascopic illumination lamphouse from the microscope.

(2) For the Eclipse 80i Remove the microscope ring (male). Use the three screws (M3×8) to clamp the 80i adapter ring (female, C1-TD-J). Use the three screws (M3×5) to clamp the male adapter ring to the lamphouse, placing the black plastic ring between the lamphouse and ring. (See Fig. V-12) Insert the transmission detector into the 80i lamphouse mounting. Use the two setscrews to clamp it into place. Next, insert the lamphouse into the transmission detector and clamp with the setscrew. Finally, connect the lamphouse cable to the 80i connector.

Fig. V-12

(3) For the E800, E1000, or E600/E600FN Use the three screws (M3) to clamp the adapter ring (female, C1-TD-U) to the lamphouse, with the black plastic ring between the lamphouse and ring. Insert the transmission detector into the microscope lamphouse mounting. Use the two setscrews to clamp it into place. Next, insert the power connector, insert the lamphouse into the transmission detector, and clamp with the setscrew. (See Figs. V-13, 14, and 15)

Fig. V-13

M3x5 (3pieces) M3x8 (3pieces)

C1-TD-J C1-TD-J

Lamphouse side

Microscope side

M3 (3pieces)

C1-TD-U C1-TD-U

Lamphouse side

Microscope side

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(4) Insert the tip of the light guide fiber that has been secured in an arc shape with a leaf spring into the transmission detector unit and secure it with two set screws.

Fig. V-14

Fig. V-15

When using the TE2000

(1) Detach the diascopic illumination lamphouse from the microscope.

(2) Secure the adaptor ring(C1-TD-I) to the transmission detector unit with a black resin ring (supplied with the C1-TD-I adaptor) in between them. Use three screws to secure the adaptor. (See Fig. V-16.)

Fig. V-16

(3) Fit the cylindrical adaptor for inverted microscope to the front of the transmission detector unit and secure it with a set screw.

(4) Fit the transmission detector unit to the lamphouse mount on the microscope.

E800

Light guide fiber

Lamphouse side Microscope side

C1-TD-I C1-TD-I

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(5) Fit the diascopic illumination lamphouse to the rear of the transmission detector unit and secure it with a set screw. (See Fig. V-17.)

(6) Fit the tip of the light guide fiber, which has been secured in an arc shape with a leaf spring, to the transmission detector unit and secure it with two set screws.

(7) Detach the TE2000 illumination cable from the power supply and tie them in a bundle with one of the supplied cable bands.

(8) Connect the illumination cable (extending out of the adaptor for inverted microscope) to the power supply.

(9) Secure part of the light guide fiber with a cable band (supplied with the TE2000 illumination cable) so that the light guide fiber is flexed.

Fig. V-17

Light guide

Tie the TE2000 illumination cable in a bundle with a cable band.

Cable from the adaptor for inverted microscopes

Secure the light guide fiber and TE2000 illumination cables with a cable band.

To the controller

To the power transformer

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II C1-ETD (motorized switch type)

When using the Eclipse 80i/90i

(1) Remove the diascopic illumination lamphouse from the microscope.

(2) Insert the light guide (optical) fiber into the transmission detector body and secure the light guide fiber with a set screw.

Fig V-18

(3) By using the leaf spring on the detector body, secure the light guide fiber in an arc shape.

(4) Remove the microscope side mount on the detector body, and then secure the cylindrical adaptor ring with three screws (M3 x 8).

(5) Secure the adaptor ring to the lamphouse side of the detector body through a black ring, attached as an accessory, with three screws (M3 x 5).

Fig V-19

(6) Fit the detector body to the lamphouse mount on the microscope.

(7) Connect the electric wire to the detector body.

(8) Route the electric wire along the light guide fiber, and then fix it with a cable band (attached as an accessory). The other edge of the electric wire will be connected to the controller. For details, refer to “X. Connecting the Cables Between Units.”

(9) Mount the lamphouse.

M3x5 (3 locations) M3x8 (3 locations)

C1-TD-J C1-TD-J

Lamphouse side

Microscope side

Light guide fiber

M3x10 (3 locations)

Remove it.

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When using the TE2000

(1) Remove the diascopic illumination lamphouse from the microscope.

(2) Insert the light guide (optical) fiber into the transmission detector body and secure the light guide fiber with a set screw.

(3) By using the leaf spring on the detector body, secure the light guide fiber in an arc shape.

(4) Secure the adaptor ring to the lamphouse side of the detector body through a black ring, attached as an accessory, with three screws.

Fig V-20

(5) Fit the cylindrical adaptor for inverted microscopes to the front of the detector body and secure it with a set screw.

(6) Fit the detector body to the lamphouse mount on the microscope.

(7) Connect the electric wire to the detector body.

(8) Route the electric wire along the light guide fiber, and then fix it with a cable band (attached as an accessory). The other edge of the electric wire will be connected to the controller. For details, refer to “X. Connecting the Cables Between Units.”

(9) Detach the TE2000 illumination cable from the power transformer and tie them in a bundle with one of the supplied cable bands.

(10) Connect the illumination cable (extending out of the adaptor ring for inverted microscopes) to the power transformer.

(11) Secure the light guide with a cable band (attached as an accessory) so that the TE2000 illumination cable is flexed.

(12) Mount the diascopic lamphouse on the back of the detector body, and then secure it with a set screw.

Lamphouse side

Microscope side

C1-TD-I C1-TD-I

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Fig V-21

Light guide fiber

Tie the TE2000 illumination cable in a bundle with a cable band.

Cable from the adaptor for inverted microscopes

Secure the light guide and electric wire with a cable band.

To the controller

To the power transformer Electric wire

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6.2 Incorporating the Electric Board and the Light Guide Fiber into the C1 Controller

6.2.1 For Transmission Detector (MHE47100)

• Before beginning the setup process, be sure to check that the C1

controller is turned OFF. Note that the plate attached to the transmission detector unit warns of potential danger of exposing the eyes and skin to the laser beam if any laser is left ON during setup.

(1) Detach the side panel of the controller. A total of four screws are used: two at the upper and lower rear, and the other two at the bottom.

(2) Remove the screw from the lowermost bracket of the controller and then detach the bracket.

(3) Loosen the two set screws on the outer metal flange extending out of the board and detach the flange-2. (See Fig. V-22.)

(4) Insert the board into the lowermost empty slot (ISA slot) with the inner metal flange extending out from the rear panel. Secure the bracket with the screw that was removed in step 2. (See Fig. V-22.)

Fig. V-22 Side view

WARNING

Controller

Screw

Set screw

Flange-2

Flange Bracket

Fit to the line-grabber board.

Slot (ISA)

T-PMT board

MHE47100

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(5) Connect the J2 on the T-PMT board with the J4 of the second line-grabber board using the supplied cable.(See Fig. V-23.)

SYNC-control board

3-SYNC cable

First line-grabber board

Second line-grabber boa

I/O board

T-PMT board

I-LOCK connector

J2

J4

Fig. V-23 Side view

• To use the transmission detector unit, the second line-grabber board

should be installed. See “Installing the Second Line-grabber Board (MHE41900)” in “IX. Controller” for details.

(6) Press the metal flange 2 removed in step 3 against the rear of the controller and secure in place with two set screws. (See Fig. V-24.)

Set screws.Be careful of the set-screw orientation.

Press the flange-2 against the rear of the controller ansecure with set screws.

Fig. V-24 Rear view

CAUTION

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(7) Insert the light guide fiber into the metal flange-2 and secure it with two set screws. (See Fig. V-25.)

(8) Reattach the side panel of the controller.

Fig. V-25 Side view

Two set screws

Light guide fiber

Flange-2

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6.2.2 For Motorized Transmission Detector (MHE47200)

• Before beginning the setup process, be sure to check that the C1

controller is turned OFF. Note that the plate attached to the transmission detector unit of motorized switch type warns of potential danger of exposing the eyes and skin to the laser beam if any laser is left ON during setup.

(1) Detach the side panel of the controller. A total of four screws are used: two at the upper and lower rear, and the other two at the bottom.

(2) Remove the screw from the lowermost bracket of the controller and then detach the bracket.

(3) Loosen the two set screws on the outer metal flange extending out of the board and detach the flange-2. (See Fig. V-26.)

(4) Insert the board into the lowermost empty slot (ISA slot) with the inner metal flange extending out from the rear panel. Secure the bracket with the screw that was removed in step 2. (See Fig. V-26.)

Fig. V-26 Side view

WARNING

Controller

Screw

Set screw

Flange-2

Flange Bracket

Fit to the line-grabber board.

Slot (ISA)

T-PMT board

MHE47200

Fit to the I/O board.

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For C1

(5) Connect the cable from the D-sub connector on the bracket to the J8 (8 pin, white) on the I/O board. (See Fig. V-27.)

(6) Connect the J2 on the T-PMT board with the J4 of the second line-grabber board using the supplied cable. (See Fig. V-27.)

SYNC-control board

3-SYNC cable

First line-grabber board

Second line-grabber boa

I/O board

T-PMT board

D-sub connector

I-LOCK connector

J2

J4

J8

Fig. V-27 Side view

• To use the transmission detector unit of motorized switch type, the

second line-grabber board should be installed. See "installing the Second Line-grabber Board (MHE41900)" in "IX. Controller" for details.

(7) Press the metal flange-2 removed in step 3 against the rear of the controller and secure in place with two set screws. (See Fig. V-28.)

Set screws.Be careful with the set-screw direction.

Press the flange-2 against the rear of the controller ansecure with set screws.

Fig. V-28 Rear view

CAUTION

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For C1si

(5) Connect the cable from the D-sub connector on the bracket to the J8 (8 pin, white) on the I/O board. (See Fig. V-29.)

(6) Connect the J2 on the T-PMT board with the J4 on the second line-grabber board using the supplied cable. (See Fig. V-29.)

SYNC-control board 4-SYNC cable

First line-grabber board

Second line-grabber board

I/O board

T-PMT board

D-sub connector

I-LOCK connector

J2

J4

J8 Spectral board

Fig V-29 Side view

• To use the transmission detector unit, the second line-grabber board

should be installed. See “Installing the Second Line-grabber Board (MHE41900)” in “IX. Controller” for details.

(7) Press the metal flange-2 removed in step 3 against the rear of the controller and secure in place with two set screws. (See Fig. V-30.)

Set screws. Be careful with the set-screw direction.

Press the flange-2 against rear of the controller and secure with set screws.

Fig V-30 Rear view

CAUTION

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(8) Insert the light guide fiber into the metal flange-2 and secure it with two set screws. (See Fig. V-31.)

(9) Reattach the side panel of the controller.

Fig V-31

Two set screws

Light guide fiber

Flange-2

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Scan Head VI

• Turn off power to all lasers and units before starting the setup

procedures described here.

• This system must be used with the specified microscope and attached to the specified section of the specified eyepiece tube. Use with non-specified items may result in accidental exposure of eyes to laser radiation through the eyepiece when the laser is turned on, with resulting injuries.

• This system must be set up by a trained service personnel in accordance with the setup manual instructions. Contact your distributor if you wish to use a different microscope.

1 Installing the Ring Adapter

Although the scan head is attached to the microscope, the position of the pupil varies depending on the type of the microscope or whether the intermediate tube are attached. Thus the ring adapter must be installed to adjust the position of the pupil. Two types of ring adapters are available. They have different lengths (L = 9 mm; S = 4 mm). They can be installed individually or in combination.

The following table shows the ring adapters used for particular combinations of a microscope and an intermediate tube.

Microscope Eyepiece tube Mounting section

Fluorescent device

Ring Adaptor

DIH (using C1-TE) Front port Built-in L Eclipse 80i/90i Dedicated trinocular

eyepiece tube Camera port U-Epi L

E1000

E800

Dedicated beam splitter Rear port Built-in None

E600 Dedicated trinocular eyepiece tube

Camera port U-Epi L

Upright microscope

E600FN Dedicated trinocular eyepiece tube

Camera port U-Epi S

TE2000E Inverted microscope TE2000U

Provided (using C1-TE) Left side port Built-in S

• Always attach the specified size ring adapter for a particular microscope

setup. Incorrect attachment will increase shading (darkening of the peripheral image field).

Remove the relay tube (screw-in type) from the scan head, then install the ring adapter under the standard ring.

WARNING

CAUTION

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Fig. VI-1

2 Securing the Scan Head in Place

2.1 For the Eclipse 80i/90i

• Be sure to install the scan head to the vertical tube section of the

trinocular eyepiece tube (MHB42200). If the scan head is mounted in an incorrect location, the laser beam may project from the eyepiece and cause eye damage.

Install the scan head so that the laser indicator is located on the front side of the microscope. Set the scan head on the vertical tube and tighten set screws C in three locations. Make sure that the ring adapter is properly inserted in the scan head.

Fig. VI-2

Laser indicator

Set screw C

Ring adapter (S)

Ring adapter (L)

Standard ring

Relay tube

Scan head

WARNING

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2.2 For the E800/E1000

• Be sure to install the scan head on the back port side of the

beam-splitter module (MAD56111). If the scan head is mounted in an incorrect location, the laser beam may project from the eyepiece and cause eye damage.

Install the scan head so that the laser indicator is located on the front side of the microscope. Set the scan head on the vertical tube and tighten set screws C in two locations. (No ring adapter is required.)

Fig. VI-3

Laser indicator

Set screw C

* No ring adapter is required.

WARNING

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2.3 For the E600/E600FN

• Be sure to install the scan head to the vertical tube section of the

trinocular eyepiece tube (MHB42200). If the scan head is mounted in an incorrect location, the laser beam may project from the eyepiece and cause eye damage.

Install the scan head so that the laser indicator is located on the front side of the microscope. Set the scan head on the vertical tube and tighten set screws C in three locations. Make sure that the ring adapter is properly inserted in the scan head.

Fig. VI-4

Laser indicator

Set screw C

WARNING

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2.4 For the TE2000-E/U

• Be sure to install the interlock module for inverted microscopes when

installing the scan head. If the interlock module for inverted microscopes is not installed, the laser beam may project from the eyepiece and cause eye damage.

Install the scan head to the special straight tube (TE mount) attached to the left side port. Install the scan head so that the laser indicator is located on the front side (first dichroic mirror inlet position at the top) of the microscope. Set the scan head on the straight tube and tighten set screws C in two locations. Make sure that the ring adapter is properly inserted in the scan head.

Fig. VI-5

First dichroic mirror inlet

Laser indicator Set screw C

Set screw C

WARNING

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3 Inserting the First Dichroic Mirror

Remove the dummy dichroic mirror from the first dichroic mirror inlet. Insert the first dichroic mirror.

Fig. VI-6

First dichroic mirror inlet

First dichroic mirror

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4 Connecting the Optical Fiber Cables

1) Remove the sheet metal cover from the back side of the scan head.

2) Connect the optical fiber cable for the excitation light by aligning the connector key with the grooved receptacle and screwing the connector key into place. Bent needle jaw pliers are useful in screwing the receptacle completely. Check the connector freeplay at the connection part.

3) Screw the optical fiber cable for the fluorescence light and the optical fiber cable for the spectral detector (with key on the connector) to connect the scan head.

4) For cable connections, refer to “X. Connecting the Cables Between Units.”

• When the optical fiber for the fluorescence light or the optical fiber for

the spectral detector is not connected, be sure to put the metal cap onto the connector. If the cap is not mounted, laser beam may project onto eyes and skin and cause damage.

Fig. VI-7

Fig. VI-8

Optical fiber for the excitation light

Optical fiber for the fluorescence light

Sheet metal cover

Optical fiber for the spectral detector

Bent needle jaw pliers

CAUTION

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Detector Unit VII 1 Spectral Detector Unit

Turn off all lasers and power supplies to perform the setup work.

Check item before the setup work The ambient temperature of this unit must be under 35°C during the setup

work. Be sure to check the temperature beforehand.

(1) Remove the optical fiber for fluorescence light.

1) Remove fiber retaining fixtures (four pieces) on the rear panel.

2) Release the optical fiber, and then put the fiber retaining fixtures back on the rear panel.

Fig VII-1

The pigtail of the optical fiber will be connected to the scan head.

(2) Replace cover plates for the optical fiber.

1) Remove the cover plate for transport on the rear panel.

2) Remove the cap on the tip of the optical fiber. And then remove the cover plate for transport from the optical fiber.(See Fig VII-2)

3) Draw the optical fiber through the opening of the cover plate included as an accessory.

4) Attach the replaced cover plate on the rear panel. Fig VII-2

Fig VII-3

(3) Wiring Refer to “X. Connecting the Cables between units.”

Removed cover plate for transport

Optical fiber retaining fixtures

Cover plate included as an accessory.

Cover plate for transport

WARNING

CAUTION

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2 Standard Detector Unit

Turn off all lasers and power supplies to perform the setup work.

(1) Connecting the optical fiber for the fluorescence light

1) Remove the flange and cap from the connection port for the optical fiber for the fluorescence light and insert the optical fiber (provided with the scan head) into the opening. Since both ends of the optical fiber for the fluorescence light are identical, either cable end may be inserted into the port.

Fig. VII-4

• Do not touch the glass section of the optical-fiber . Staining the glass may degrade image quality.

2) Securely screw one end of the optical fiber for the fluorescence light into the receptacle in the detector unit.

3) Reinstall the flange and cap.

NOTE The opposite end of the optical fiber for the fluorescence light is connected to the scan head after the detector unit is checked, as described in “XI. General Adjustment.”

Fig. VII-5

(2) Cable connections Refer to “X. Connecting the Cables Between Units.”

(3) Three-detector unit (MHE41300) The three-detector unit requires the addition of the second line grabber board (MHE41900). (See the section describing the setup of the three-detector unit in “IX. Controller” and “IV. Setting Up the PC and Operation Software.”)

CAUTION Do not touch the glass section of the tip.

Connection port

Flange and cap

Fluorescent-light optical-fiber

CAUTION

WARNING

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Laser Unit VIII

• The unit must be set up as instructed by the local LSO (Laser Safety

Officer).

• This laser unit uses Class IIIB lasers. Accidental exposure of eyes or skin to laser light may result in injury or other problems. Do not turn on the lasers until they are installed in the laser unit

• Confirm that there is no one in the area before turning on the lasers.

• Always wear laser-protection goggles while lasers are turned on.

• Do not place tools or optical components such as mirrors in the light path while lasers are turned on.

• Use the lowest output power rating during setup. For Ar lasers, use standby mode during setup.

• The laser units are heavy. Without the lasers installed, the two-laser unit S and the two-laser unit weigh approximately 13 kg, the three-laser unit S and the three-laser unit weigh approximately 27 kg, and the three-laser unit EX weighs approximately 24 kg. Do not attempt to lift laser units unassisted.

• The laser manual must be provided to the user.

To use a laser diode or a solid-state laser with the spectral detector unit, its wavelength must be measured with a spectrometer and registered into the EZ-C1 beforehand.

WARNING

CAUTION

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1 System Description

1 Two-laser unit S and two-laser unit

Attach two of the lasers listed below to the unit in the combination of (1) and (2). To control laser intensity, insert or remove the two ND filters listed below in front of each laser head. Each laser is equipped with a software-controlled motorized shutter.

2 Three-laser unit S and three-laser unit

Attach three of the lasers listed below to the unit in the combination of (3), (4) and (5). To control laser intensity, insert or remove the two ND filters described below for each laser head. Each laser is equipped with a software-controlled motorized shutter.

3 Three-laser unit EX

Attach three of the lasers listed below to the unit in the combination of (6), (7), and (8). To control light intensity, use the light control knob for each laser head, or attach the optional AOM unit for software control. Keep in mind that the 408 variable and the R-LD are adjustable through software, even without the AOM unit. (But the AOM controller is required.) Each laser is equipped with a software-controlled motorized shutter. Optional Ar wavelength selector units permit selection of either 488 nm or 514 nm wavelength output.

Two-laser unit Stwo-laser unit

Three-laser unit S three-laser unit

Three-laser unit EX

Coherent Model RADIUS405 (405) - (3) (6)

Melles Griot Model 56ICS 423 (408) - - (6)

Melles Griot Model 56ICS 426 (440) - - (6)

Spectra-Physics Model 163-C02 (488/514) - - (7)

Spectra-Physics Model 163-C12 (488) - (4) (7)

Spectra-Physics Model 161C-030 (488) (1) (4) (7)

Melles Griot Model IMA101040 (488/514) (1) (4) (7)

Coherent Model Sapphire-488-20 (488) - - (7)

Melles Griot Model 25LGP193 (543) (2) (5) (8)

Melles Griot Model 85YCA010 (561) - - (8)

JDP Uniphase Model 1677P (594) (2) (3) (6)

JDP Uniphase Model 1125P (633) - (3) (6)

Melles Griot Model 56ICS 272 (635) - - (6)

Melles Griot Model 56ICS 264 (640) - - (6)

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(9) ND filter slider

ND4 Slider status and transmittance OUT IN

OUT 100% 25%

ND8 IN 12.5% 3.1%

(10) Single-mode fiber lead-in section Provided to connect to the single-mode fiber

(11) Light control knob (optional AOM) This knob controls light intensity in 11 discrete steps or continuously. You can also use the optional AOM unit to control light output. Use the transmittance figures as a guide when using an Ar laser. Laser focus adjustments will significantly affect transmittance when using the RADIUS405. Adjust focus carefully, referring to "3.2 Laser Installation."

Transmittance (%) 100 80 50 25 10 3.5 1.3 0.5 0.15 0.05 0.02

(12) Ar wavelength selector (optional) Select one of two Ar laser wavelengths (488 nm or 514 nm).

(13) Motorized shutter connector Used to open/close the built-in motorized shutter via software to emit or cut off laser emission.

(14) AOM connector (optional) Used to control the built-in AOM unit via software to vary laser intensity.

(6)

(7)

(10)

(8)

(13) (12)

(11)

(11)

(14)

(11)

(5) (4)

(3)

(9)

(10)

(2)

(1)

(9)

(10)

(13)

(13)

Fig. VIII-2

IN: Push in OUT:Pull out

Fig. VIII-1

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2 Two-laser Unit S, Two-laser Unit, Three-laser Unit S, and Three-laser Unit

The setup procedures for the two-laser unit S, two-laser unit, three-laser unit Sand three-laser unit are given below.

(1) Preparation

(2) Laser installation

(3) Laser rough adjustment

(4) Final adjustment for transmitting Ar laser light through the optical fiber

(5) Final adjustment for transmitting He-Ne laser and 405 laser light through the optical fiber

(6) Attaching the sheet metal covers

2.1 Preparation

1 Checking accessories

Confirm that the following accessories have been provided:

• Cable (×1)

• Hexagonal head screws (×3)

• Harness bands (×6)

• Harness band retainers (×4)

• Label (×1)

• Black sponges (×2)

2 Removing sheet metal covers

Remove the screws and detach the sheet metal covers.

Two-laser unit S andtwo-laser unit

Three-laser unit S and three-laser unit

ND filterTop sheet metal cover

Side sheet metal cover

Adjuster

Fig. VIII-3

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2.2 Laser Installation

Install the lasers shown in the table described in “1. System Description” onto the specified positions.

1 Ar laser installation

1) Loosen the screw on the mounting plate attached to the laser unit and remove the mounting plate. Fasten it securely to the bottom of the head using the screw provided with the laser head.

2) Place the Ar laser at the specified position within the laser unit and secure temporarily using the screw above.

2 He-Ne laser installation

1) Loosen the sleeve-retaining screws (3 locations) and slot-tightening screw (1 location) to allow insertion of the laser head.

2) Insert the front edge of the laser head into the two sleeves. To do this, push down the plunger projecting from the bottom of the sleeve. To prevent leakage of scattered laser light, press the edge of the laser head against the sponge ring attached to the plate just enough to compress the ring slightly. When inserting the laser head through the sleeves, carefully insert in the slotted sections to keep the shutter section on the laser head from catching.

3) Turn the set screw on top of the block to adjust the laser head position to the center of the opening in the mounting block.

3 405 laser installation (for RADIUS405)

Use the focus adjustment tool provided with the laser to adjust the laser to give a minimum beam diameter at 1 m distance from the emitter.

1) Loosen the sleeve-retaining screws (3 locations) and slot-tightening screw (1 location) to allow insertion of the laser head clamp.

2) Attach the clamps to the laser head (2 locations, front and back).

3) Remove the rear mounting block. (*2)

4) Insert the clamp on the front end of the laser head into the sleeve fitted to the front mounting block.

5) Insert the clamp on the rear of the laser head into the sleeve on the rear mounting block. Make sure the label is facing up. (The major axis of the beam is horizontal. Note that axis of the beam from the three-laser unit EX is vertical.)

4 Laser warm-up

1) Open the shutters on each laser head and check that the laser unit shutter is closed. Turn on the lasers with both of the ND filters for each laser set to "IN."

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NOTE: Leave the lasers turned on for approximately 10 minutes before adjusting. Making adjustments immediately after turning on the lasers may result in shifts from adjusted levels.

Fig. VIII-4

Two-laser unit S and two-laser unit

Three-laser unit S and three-laser unit

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2.3 Laser Rough Adjustment

Determine the position of the argon laser by using the optical axis of the farthest He-Ne laser (He-Ne1 laser in Fig. VIII-5 or in Fig. VIII-6) as the reference. Align the lasers so that their laser beams can be transmitted through the optical fiber for the excitation light.

1 Setting up the dichroic mirror centering tool

Remove the dichroic mirrors and mount the dichroic mirror centering tool (see Fig. VIII-7). Install the lasers so that their incident points are on the tool.

Remove the dichroic mirrors.

He-Ne1

Ar

He-Ne2

Ar

He-Ne1

Fig. VIII-6

Fig. VIII-5

Fig. VIII-7

After the dichroic mirror of the He-Ne2 laser has been removed

Pinhole for mounting the dichroic mirror centering tool

Dichroic mirror centering tool

Remove the dichroic mirrors.

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2 Adjusting the lasers

[Adjustment of the argon laser]

1) Mount the dichroic mirror centering tool at the location of the dichroic mirror for the argon laser (see Fig. VIII-7 and Fig. VIII-8).

2) Open the shutter for the argon laser.

3) Adjust the horizontal position by matching the securing position along the screw hole on the mounting plate with hole L on the dichroic mirror centering tool. For vertical positioning, adjust the hole on the dichroic mirror centering tool so that the argon laser is aligned with hole L on the centering tool. (The height can be adjusted by holding one side of the dichroic mirror centering tool and rotating the other side. See Fig. VIII-9.)

4) After adjustment, tighten the four hexagonal socket-head bolts on the argon laser.

5) Close the shutter for the argon laser.

[Adjustment of the He-Ne laser]

Mount the dichroic mirror centering tool after you have adjusted its height using the argon laser at the location of the dichroic mirror for the He-Ne laser, as shown in Fig. VIII-11, and adjust each He-Ne laser.

1) Open the shutter for the He-Ne laser. (Insert the red rubber cap placed on the tip of the excitation-light optical-fiber cable between the shutter and the sheet metal cover to allow the shutter to be held open. See Fig. VIII-10.)

2) With the rear mount of the He-Ne laser positioned at the center, adjust the front-mount centering adjustment screws with hole L on the dichroic mirror centering tool. The centering accuracy should be within 0.2 mm.

3) Close the shutter for the He-Ne laser.

4) For the three-laser unit, repeat the adjustment procedure for the other He-Ne laser.

Adjust the height of the dichroic mirror centering tool.

Dichroic mirror centering tool

Fig. VIII-8

1) Hold to prevent movement

2) Rotate here while holding the upper section to adjust the height

hole R hole L

1mm Shutter

Fig. VIII-9

Shutter Rubber cap

Sheet metal coverFig.VIII-10

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3 Installing the dichroic mirror for the He-Ne laser

As shown in Fig. VIII-12, install the dichroic mirror for each He-Ne laser. (Do not attach the dichroic mirror for the argon laser.)

• For the three-laser unit, the dichroic mirror for the He-Ne laser located farthest from that for the argon laser has a total reflection surface, while the middle dichroic mirror has a green reflection surface.

• For the two-laser unit, the dichroic mirror for the He-Ne laser located farthest from that for the argon laser has a total reflection surface.

He-Ne2

Dichroic mirror centering tool.

Shutter Adjust only the front mount to align with the centering tool.

Fig. VIII-11

Adjust the dichroic mirror to align with the centering tool.

Dichroic mirror centering tool

Fig.VIII-12

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4 Adjusting the angle of the dichroic mirror for the He-Ne laser

Change the direction of the dichroic mirror centering tool re-mounted at the argon laser location. Referring to Fig. VIII-13, adjust the angle of the dichroic mirror for the He-Ne laser so that the beam of the He-Ne laser located farthest from the argon laser is aligned with hole R (Refer to Fig. VIII-9).

1) For horizontal adjustment, slightly loosen hexagonal socket-head bolts a1, insert a screwdriver or thin stick into a2 to permit tilting, then rotate the entire mirror. For vertical adjustment, slightly loosen set screws b1, insert a screwdriver or thin stick into b2 to permit tilting, then rotate the entire mirror.

2) When the dichroic mirror is properly angled, tighten hexagonal socket-head bolts a1 and set screws b1 to secure the mirror in place. (Simultaneous tightening of the two screws minimizes positional deviation.)

3) Adjust the accuracy of the dichroic mirror angle within ±0.2 mm from centering tool hole

R. (Make the adjustment carefully, to ensure smooth entry of the laser beam into the optical-fiber cable.) For the three-laser unit, the adjustment of the dichroic mirror for the He-Ne1 laser will be made in the final adjustment , rather than at this step.

Fig. VIII-13

5 Setting up the dichroic mirror for the argon laser

Mount the dichroic mirror for the argon laser on the argon laser section. This dichroic mirror has a blue reflection surface.

a2a1b1b2

a2Set screws b1

b2

Hexagonal socket- head bolts

a1

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6 Centering the He-Ne laser

As shown in Fig. VIII-14, place the target tool at a distance of about 50 cm from the coupler. To perform adjustment of the target tool, move its pinhole so that the beam of the He-Ne laser (He-Ne2 with the three-laser unit, He-Ne1 with the two-laser unit) farthest from the argon laser is aligned with the pinhole of the target tool. The pinhole used for centering should be slightly narrower than the laser beam. The centering accuracy should be within 0.1 mm.

The target tool position can be adjusted with the two set screws. The setup condition of the target tool varies by installation location. When selecting a location, make sure that the laser beam can reach the target tool.

Fig. VIII-14

7 Centering the argon laser beam

Align the argon laser beam with the pinhole of the target tool that has been adjusted with the He-Ne2 laser. Adjust the dichroic mirror for the argon laser so that the argon laser beam aligns with the pinhole of the target tool that has been adjusted with the He-Ne2 laser. The accuracy should be within 0.4 mm.

Do not look directly at the incident laser light during adjustment.

1) Target tool Align the pinhole position with the He-Ne2 laser beam.

He-Ne2

Ar

He-Ne1

Centering screw

pinhole

Target tool

2) Adjust the dichroic mirror for the argon laser and align with the pinhole.

3) Adjust the dichroic mirror for the He-Ne1 laser and align with the pinhole.

50cm

WARNING

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8 Centering the He-Ne1 laser beam in the middle

If the He-Ne1 laser is located in the middle of the enclosure, adjust its dichroic mirror so that the He-Ne1 laser beam aligns with the pinhole of the target tool set in the previous step. The accuracy should be within 0.8 mm.

9 Checking the screws

Make sure that all the dichroic mirror adjustment screws are firmly tightened.

10 Aligning polarization of lasers

This section describes how to align the direction of polarization of the He-Ne laser with reference to that of the argon laser. (See Fig. VIII-15.)

1) Place a polarizer-equipped tool at the end of the argon-laser dichroic mirror. At this time, place the polarizer-equipped tool with its flat surface at the back, as shown in Fig. VIII-15.

2) Place a light-scattering target (such as paper) at the end of the coupler and then move the knob on the polarizer-equipped tool to determine the position at which the laser beam is the darkest. (At this time, remove the two ND filters from the argon laser. This will make it easier to locate the darkest position.) At this time, the direction of polarization of the argon laser is as shown in Fig. VIII-15.

3) Place a light-scattering target (such as paper) at the end of the fiber mount and then rotate the He-Ne laser to determine the position at which the laser beam is the darkest. (At this time, remove the two ND filters from the He-Ne laser. This will make it easier to locate the darkest position.) At this time, the polarizer must be oriented in the same direction as that of the argon laser. At this time, the direction of polarization of the He-Ne laser is as shown in Fig. VIII-15, where the polarization directions of argon laser and He-Ne laser are aligned. Adjust carefully within the range of approximately ±2 degrees. (A discussion of the direction of

laser polarization is included in the laser instruction manual. Refer to the manual for rough configuration of the lasers. This will make it easier to determine the direction of polarization.)

4) For two He-Ne lasers system, adjust the polarization direction of the second laser in the same manner.

5) Rotating the He-Ne laser may displace it from the pinhole of the target tool adjusted as directed in “6 Centering the He-Ne laser” in this section. If this occurs, adjust the set screws L1 and R1 provided at the He-Ne laser radiation side as shown in Fig. VIII-15 to align the laser with the target.

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Fig. VIII-15

11 Laser intensity measurement

At first, maximize each laser intensity. Move off all of movable ND filters from the optical path and adjust Argon laser to its maximum power. Measure and record the light intensity of each laser emitting from the coupler with a calibrated laser intensity power meter.

This completes the rough setup of the lasers.

Polarizer-equipped tool (Push the tool until its edge surface touches the dichroic mirror.)

He-Ne2

Ar

He-Ne1

Adjusting screw on the incoming laser side

Adjusting screw on the fiber

Coupler

Target (such as paper)

Direction of polarization of polarizer

Rotate polarizer so that the argon laser becomes darker.

Direction of polarization of laser

A B

R1

L1 R1

L1

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2.4 Final Adjustment for the Ar Laser Light with the Optical Fiber

1 Centering the Ar laser

Align the Ar laser beam with the coupler centering tool pinhole.

1) Loosen the clamping screw on the coupler unit.

2) Set the coupler centering tool pinhole in the laser incident direction, place a paper on the emission side, and adjust the H and V laser incidence side adjustment screws to find the point giving maximum brightness.

3) Set the coupler centering tool pinhole in the direction of laser emission and adjust the H and V fiber side adjustment screws to find the point giving maximum brightness.

4) Repeat steps 2) and 3) two or three times to find the point of maximum brightness.

Laser incidence side adjustment screw

Fiber side adjustment screw

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2 Attaching the fiber coupling

1) Close all laser shutters.

2) Tighten the three screws and attach the fiber coupling unit. Since this will be rotated at a subsequent step, do not tighten excessively at this time. Tighten so that the units can still be rotated by inserting the tool in the tool opening. Make sure that the key slot in the fiber coupling is at the bottom (different from EX). Handle the coupling carefully to keep out debris.

3 Transmitting Ar laser light through the fiber

Never look directly at the fiber tip. Always monitor laser emissions by aiming the tip down onto a sheet of paper.

1) Carefully align the end of the fiber (the end without the tag) with the key slot in the coupling and insert as far as it will go. Tighten the fiber connector. Gradually tighten, stopping when light is first emitted from the tip of the fiber.

2) If no light is emitted from the end of the fiber in step 1), loosen the fiber connector approximately 10 degrees, gradually pull out the fiber, and observe whether light is emitted. (Light is emitted more easily if defocused.) If this is not effective, repeat the steps of loosening the connector and pulling the fiber a couple of times.

WARNING

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3) If even a small amount of laser light is observed, adjust the two fiber side adjustment screws on the coupling to find the point at which the Ar laser light increases in intensity. (Final fine adjustment will be performed after adjusting the polarizing orientation.)

4) Gradually tighten the fiber connector. Stop just before the point at which light is no longer emitted. Now adjust the two fiber side adjustment screws to find the point giving a maximum light intensity. Repeat this procedure to ensure a maximum intensity, checking to confirm that the fiber connector is tightened.

5) If no light is emitted even after repeating these steps, repeat the procedure from step 1 in “2.4 Final Adjustment for the Ar Laser Light with the Optical Fiber.”

6) Tighten the fiber connector at the point giving a maximum Ar laser light brightness. Check that there is no play in the fiber.

7) Close the Ar laser shutter.

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4 Adjusting the fiber polarizing orientation

1) Align the knob on the polarizer-equipped tool with the index. Mount the polarizer-equipped tool on the polarizing orientation adjustment tool with the knob at the top.

2) Clamp the emission end of the optical fiber to the polarizing orientation adjustment tool. Place a light-scattering target (such as paper) before it. Position the fiber so that it is not subjected to excessive force, and so that both ends are laid out straight.

3) Open the Ar laser shutter. Insert a tool into the coupling and rotate while monitoring the laser light image on the target to find the point giving the minimum brightness. (If the three screws on the coupling are too tight, loosen them slightly before rotating.)

4) Place the power meter in front of the polarizer to measure the laser power.

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5) Rotate the polarizer-equipped tool by 90 degrees so that the knob faces to the side, place the power meter in front of the polarizer, and measure laser power.

6) Check that the extinction ratio (ratio of bright to dark laser power) is at least 20:1. If not, repeat the adjustment procedure from step 1.

7) Tighten the three clamping screws on the

coupling.

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5 Adjusting to give maximum Ar laser light transmission through the fiber

1) Attach the emitter end of the optical fiber to the power meter tool and adjust to find the point giving maximum brightness, while measuring the brightness on the power meter. To adjust brightness, use either the laser incidence side H adjustment screw (horizontal) or V adjustment screw (vertical), together with the fiber side V adjustment screw and H adjustment screw. As a guide to Ar laser incidence efficiency, the brightness at the end of the coupler should be at least 50% of the brightness in front of the coupler.

(Transmitting the light through the AOM unit will distort the beam shape and result in a lower transmission ratio than without the AOM unit.)

(Adjustment example)

(1) Turn the laser incidence side H adjustment screw 30 to 60 degrees in one direction.

(2) Turn the fiber side H adjustment screw in the same direction as (1) to find the point giving a maximum brightness. If brightness does not increase, turn the laser incidence side adjustment screw 30 to 60 degrees in the direction opposite to (1). Turn the fiber side adjustment screw in the same direction to find the point giving a maximum brightness.

(3) Repeat the adjustment in step (2) to find the point in the horizontal axis giving a maximum brightness.

(4) Turn the laser incidence side V adjustment screw 30 to 60 degrees. Turn the fiber side V adjustment screw in the same direction to find the point giving a maximum brightness.

(5) Repeat the adjustment in step (4) to find the point in the vertical axis giving a maximum brightness.

(6) Repeated adjustments along the horizontal and vertical axes may result in even greater brightness.

Fiber side adjustment screw Laser incidence side adjustment screw

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2) Record the Ar laser brightness. (Note the power meter wavelength setting.)

3) Open the other laser shutters and check that the laser light is emitted. If no light is emitted from the other lasers, repeat the adjustments from P.84, “6. Centering the He-Ne laser” in “2.3 Laser Rough Adjustment.”

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2.5 Final Adjustment for the He-Ne Laser Light and 405 Laser Light with the Optical Fiber

1 Transmitting the He-Ne1 laser beam through the optical-fiber cable

Without touching the coupler, adjust only the laser body to transmit the laser beam through the optical-fiber cable. (Fig. VIII-16) Incident light efficiency of more than 50% should be achieved at the emitting end of the optical-fiber cable relative to values measured in front of the coupler.

1) Close the shutters for the argon and He-Ne2 lasers so that only the He-Ne1 laser beam enters the optical-fiber cable.

2) Mount the end of the optical-fiber cable onto the power meter tool and measure the light intensity with the power meter.

3) Adjust the front and rear set screws on either the right or the left side of the laser. (Front set screw R1 and rear set screw R2 on the right side are adjusted by the following example.)

(1) Rotate the front set screw R1 (close to the laser-emitting end) 30 to 60 degrees in either direction.

(2) Rotate the rear set screw R2 (on the same side as the set screw rotated in step (1)), located on the back side of the laser, in the same direction as in step (1). Adjust the laser body position at which the power meter indicates a greater light intensity.

(3) If light intensity does not increase, rotate the set screws R1 and R2 (in that order) in the opposite direction of the rotation in step (1). Adjust the laser body position at which light intensity becomes greater.

(4) Repeat steps (2) and (3) to adjust the position at which light intensity is greatest.

4) Adjust the front and rear set screws on the other side of the laser and locate the laser body position at which light intensity is greatest. (Front set screw L1 and rear set screw L2 on the left side, in this example.)

5) When set screws R1 and R2 are adjusted again, the light intensity may be further increased.

6) Record the light intensity.

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Fig. VIII-16

2 Transmitting the He-Ne2 laser beam through the optical-fiber cable

As with the He-Ne1 laser, adjust the He-Ne2 laser body position to transmit the laser beam at maximum light intensity through the optical-fiber cable. At this time, close the shutters for the argon and He-Ne1 laser so that only the He-Ne2 laser beam enters the optical-fiber cable. Incident light efficiency of about 50% should be achieved at the emitting end of the optical-fiber cable relative to values measured in front of the coupler. Record the light intensity.

3 Attaching the He-Ne laser to the holder

1) While pressing the sleeve against the sleeve-mounting section, lightly tighten the slot-tightening screw. During this process, light intensity may decrease slightly. Work carefully, while monitoring the light intensity at the end of the optical-fiber cable on the power meter.

2) Tighten the front and rear sleeve-retaining screws of each laser.

He-Ne2

Ar

He-Ne1

Rotate screw R1 first. Note the rotating direction and turning angle.

Set screw L1

Rotate R2 after R1 and in the same direction as R1.

Set screw L2

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Fig. VIII-17

4 Laser intensity measurement

At first, maximize each laser intensity. Move off all of movable ND filters from the optical path and adjust Argon laser to its maximum power. Measure and record the light intensity of each laser emitting from the optical fiber with a calibrated laser intensity power meter.

5 Handling of cables and codes.

Fix cables and codes with bundling band.

2.6 Attaching the Sheet Metal Covers

Install the sheet metal covers.

This completes the laser adjustment.

He-Ne2

Ar

Sleeve-mounting section

Sleeve

Sleeve-retaining screw

Slot-tightening screw

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3 Three-laser Unit EX

The setup procedure for the three-laser unit EX is given below. Note that the setup procedure will differ, depending on whether or not the AOM unit is used.

(1) Preparation

(2) Laser installation

(3) Laser rough adjustment 1

* The AOM unit must be installed after the controller setup when using the AOM unit (see "IX. Controller").

(4) Laser rough adjustment 2

(5) Final adjustment for transmitting Ar laser light through the optical fiber

(6) Final adjustment for transmitting He-Ne laser and 405 laser light through the optical fiber

(7) AOM controller adjustment (only when using AOM unit)

(8) Attaching the sheet metal cover

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3.1 Preparation

1 Checking accessories

Confirm that the following accessories have been provided:

• Cable (×1)

• Hexagonal head screws (×7)

• Harness bands (×6)

• Harness band retainers (×4)

• Label (×1)

• Black sponges (×2)

2 Knob position adjustment

Turn the light control knob (white line) to the MAX position on the stepped scale.

3 Removing the sheet metal cover

Remove the six screws and the sheet metal cover.

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4 Attaching the black rings (provided)

1) Remove the bolts and nuts attached at three locations on the laser mounting position opening, holding them to avoid dropping them.

2) Attach the black (sponge) rings provided, centering them on the opening.

5 Removing the fiber coupling

Remove the three external screws and remove the fiber coupling. Handle the coupling carefully to prevent contamination of the space inside.

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3.2 Laser Installation

Overall laser unit diagram DSCN103

1 Installing the Ar laser

1) Detach the mounting plate from the laser unit.

2) Attach the mounting plate to the Ar laser. Note that the mounting plate screw positions differ for the 488 multi-line Ar (black) and 488 single-line Ar (white).

• Front three holes: 488 multi-line Ar

• Rear three holes: 488 single-line Ar

3) Place the Ar laser at the specified position in the laser unit. Be sure to press the laser emitter end against the black ring just enough to compress the ring to prevent leakage of scattered laser light. Temporarily secure the Ar laser to the laser unit using the screws.

4) Check that the laser head shutter is closed (at the CLOSE position).

Ar laser He-Ne laser 405 laser (cylindrical type)

Black ringAttach while pressing against the black ring just enough to compress it.

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2 Installing the He-Ne laser and 405 laser (cylindrical type)

1) Loosen the slot-tightening screw and three sleeve-retaining screws to allow insertion of the laser head.

2) Insert the He-Ne laser head into the sleeve. To prevent leakage of scattered laser light, press the end of the laser head against the black ring attached to the plate just enough to compress the ring slightly. Make sure that the label on the He-Ne laser is facing upward when installed.

3) 405 (for cylindrical type)

• Use the focus adjustment tool provided with the laser to adjust the laser to give a minimum beam diameter at 1.5 m to 1.7 m distance from the emitter. (This must be performed to increase transmission efficiency across the fiber connection and to ensure that the light controller is operational.)

• Insert the 405 laser head into the sleeve, making sure that the emitter end adapter is pressed against the plate. Confirm that the label on the 405 laser faces to the outer side when installed (elliptic shaped beam is in vertically oriented).

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4) Adjust the positions of the units so that the sleeve side face aligns with each mount.

5) Check that the laser head shutters are closed (at the CLOSE position).

6) Partially tighten the slot-tightening screw and one of the three sleeve-retaining screws loosened in step 1).

3 Installing the 408 laser (rectangular type)

1) Detach the two cylindrical laser holders.

2) Use the four screws provided to attach the C1-LAR408 laser adapter R.

3) Use the three screws provided to attach the 408 laser (rectangular type).

4 Laser warm-up

1) Turn on the lasers.

2) Allow the lasers to warm up for 10 minutes.

NOTE: Leave the lasers turned on for approximately 10 minutes before adjusting. Making adjustments immediately after turning on the lasers may result in shifts from adjusted levels.

3) Set the power to minimum (Standby mode) for the Ar laser.

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3.3 Laser Rough Adjustment 1

Overall view from above DSCN150

Fig. VIII-18

1 Checking the initial beam shift unit position

Check that the horizontal set screw tool openings on the sides of the two beam shift units are perpendicular to the light beam, and that the cylindrical glass section is not significantly tilted.

(They are set to these positions before shipping.)

Light control unit

Glass section

Beam shift unit

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2 Installing the dichroic mirror centering tool (for the EX)

1) Remove the screw, then remove the three dichroic mirror adjustment spring units. Take care to keep the springs from flying off.

2) Remove the two screws (total 6) on each unit, then remove the three dichroic mirror adjustment units.

Spring unit

Dichroic mirror adjustment unit

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3 Adjusting the laser installation position

3-1 Adjusting the Ar laser

1) Install the dichroic mirror centering tool (for the EX) on the Ar laser dichroic mirror unit, making sure that the tool opening faces in the direction in which the laser is emitted.

2) Open the Ar laser shutter (set to OPEN position). Open the Ar laser optical path shutter on the laser unit. The shutter can be kept open by inserting an Allen key here.

3) Adjust the rotation direction by inserting a tool into the Ar mirror adjustment hole to vertically align the laser beam and dichroic mirror centering tool opening (for the EX).

Dichroic mirror centering tool (for the EX)

Adjustment hole

Ar mirror unit Clamp screw

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4) Move the entire Ar laser unit to horizontally align the laser beam and dichroic mirror centering tool opening (for the EX).

5) Repeat the adjustments in steps 3) and 4) until the laser beam passes through the center of the dichroic mirror centering tool opening (for the EX). The centering tolerances should be within approximately 0.2 mm. Centering can be easily checked by holding a sheet of white paper behind the tool opening.

6) On the continuous control range, turn the light control knob to MAX. Confirm that the optical path is completely clear. After checking, return the knob to MAX in the step control range to adjust the laser. (See 3.1-2)

7) Tighten the Ar mirror clamp screw and the four mounting plate screws to clamp the Ar laser into place.

8) Close the shutter on the laser unit.

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3-2 Adjusting the He-Ne laser and 405 cylindrical laser

1) Install the dichroic mirror centering tool (for the EX) on the dichroic mirror unit.

2) Open the shutters for the He-Ne laser and 405 cylindrical laser (Set to OPEN position). Open the He-Ne laser optical path shutter on the laser unit. The shutter can be kept open by inserting an Allen key here.

3) Adjust the 405 cylindrical laser orientation so that the beam cross-section forms an upright ellipse.

4) Adjust by moving the rear mount with the front mount fixed so that the laser beam passes through the center of the dichroic mirror centering tool opening (for the EX). If this cannot be accomplished by moving just the rear mount, the front mount may also be moved.

5) On the continuous control range, turn the light control knob to MAX. Confirm that the optical path is completely clear. After checking, return the knob to MAX in the step control range to adjust the laser. (See 3.1-2)

6) Tighten the two sleeve-retaining screws below the center, leaving the sleeve-retaining screw above the center and the slot-tightening screw loose. This permits later adjustment of laser polarization by rotating the entire laser. (No polarization adjustment is required for the 405 cylindrical laser.) The centering tolerance should be within approximately 0.2 mm.

7) Adjust the other laser in the same way.

8) Close the shutter on the laser unit.

When the AOM unit will not be used, this completes the rough adjustment of the lasers. Proceed to "3.4 Laser Rough Adjustment 2." To use the AOM unit, set up the controller referring to the "IX. Controller" section, then proceed to the sections from "4. Attaching the AOM unit" to "7. Adjusting the AOM unit."

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4 Attaching the AOM unit

1) Turn off power to all units.

2) Remove the tape covering the opening on the AOM unit.

3) Detach the two light control units on the laser optical path to which the AOM unit will be attached, and attach the two AOM units using the three screws provided. Standard positions for attaching AOM unit are on the Ar laser unit and the G-He-Ne laser unit.

4) Detach the plate on the laser unit sheet metal unit and attach the AOM cable connector with a panel provided.

5) Connect the AOM cables to the AOM unit, routing AOM1 to the Ar laser and AOM2 to the G-He-Ne laser.

6) Wire the cables as shown in the diagram and secure with cable retainers.

AOM cable connector

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5 Wiring the AOM controller

1) Attach the SYNC panel provided to the C1 controller. (See "4. AOM Unit SYNC Cable Connection" in "IX. Controller.")

2) Connect the SYNC cable provided to the SYNC connectors on the AOM controller and C1 controller (connected in step 1).

3) Connect the RF cable provided to the AOM connectors on the AOM controller and laser unit.

4) Connect the PC cable to the PC PCI-6711 connector and AOM controller PC connector. (See the section on "IV. Setting Up the PC and Operation Software" for detailed information on installing the PCI board.)

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6 Adjusting the AOM controller adjuster knob

1) Confirm the AOM controller power switch is turned off, then remove the screws and the AOM controller cover.

2) Using the tip of a screwdriver, remove the silver caps on the AOM driver PWR adjuster (PWRADJ) and BIAS adjuster (BIASADJ).

3) Insert the tip of a small screwdriver into the PWR and BIAS adjusters and slowly turn the adjuster screws all the way counter-clockwise.

AOM1

AOM2

AOM1

AOM2

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7 Adjusting the AOM unit

1) Loosen the X-axis, Z-axis, and rotation clamp screws on the AOM unit.

2) Open the laser shutter, and adjust along the X-and Z-axes so that the laser beam passes through the AOM opening. Then, turn off the power for the AOM controller to pass the 0-order light. Adjust in the Z-axis using the adjuster screw after loosening the clamp screw completely. The transmission ratio of after-AOM to pre-AOM should be approximately 95%.

3) Detach the laser unit front sheet metal cover.

4) Turn off the AOM controller REMOTE switch. (If the REMOTE switch is in the ON position, the AOM controller will be turned on automatically when the PC is turned on. If the REMOTE switch is in the OFF position, the AOM controller can be turned on or off independent of the PC.)

X-axis clamp screw

Rotation clamp screw

Z-axis clamp screw

Rotation adjuster screw

Z-axis adjuster screw(set screw)

X-axis clamp screw

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5) Adjust the AOM rotation direction.

The AOM controller is connected to the primary power supply. Be careful to avoid touching the primary side.

When the AOM controller is turned on, laser light passing through the AOM unit is split into multiple beams. The light transmitted when the AOM controller is off is called the zero-order light. When the power is turned on, the first-order light, the second-order light, and the third-order light appear on either side of the zero-order light. Loosen the rotation direction clamp screw.

Adjust the AOM unit rotation direction so that the first-order light on the left of the laser emitter when viewed from the front is the maximum. Place a power meter about 50 cm from the AOM unit so that it is struck only by the first-order light.

Before measuring the first-order light, check the position of the zero-order light with the AOM controller turned off. The brightness may increase slightly when the AOM unit transmission opening is fine-adjusted along the X- and Z-axes.

Use the first-order light for all subsequent adjustments.

6) Carefully tighten the X-axis, Z-axis, and rotation clamp screws on the AOM unit.

Second-order light

First-order light

Zero-order light

First-order light

Second-order light

Use the first-order light.

WARNING

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8 Adjusting the AOM driver

8-1 Software setup

1) Install the PCI-6711 driver software.

2) Select Configure --> confocal --> Bleach --> Advanced from the menu after launching EZ-C1. Select 1 for Device number.

3) Select Configure --> confocal --> laser from the menu. Set the lasers. For information on making these settings, see "4.2 Laser-type Setting" in "IV. Setting Up the PC and Operation Software."

4) Display the Laser Control Bar. Select Tools Laser Control Bar from the menu.

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8-2 AOM driver PWR adjustment

1) Open [Setup] for the laser to be set up. Set [Laser Power] to <uncalibrated>. Set [Type] to <linear range>. Next, select [Close]. (The Laser Control Bar displays the voltage.)

2) Open the shutter of the laser to be set. Either set [Shutter] to <Open> or force open the laser unit shutter.

3) Set the Laser Control Bar voltage to +1V.

4) Measure the first-order light intensity using the power meter to determine the maximum brightness. Insert a fine screwdriver (like the screwdriver provided with the PCI-6711) into the PWR adjuster and turn clockwise very slowly. The maximum brightness will be achieved almost immediately for Ar lasers. G-He-Ne lasers will require a small amount of rotation, and R-He-Ne lasers somewhat more.

5) Turn the PWR adjuster counter-clockwise from the position of the maximum brightness. For Ar and G-He-Ne lasers, reduce to approximately 98% of the maximum brightness. For R-He-Ne lasers, reduce to approximately 80% of the maximum brightness. PWR adjustment is finalized where the adjuster turns in the clockwise direction. To make fine adjustments, briefly rotate counter-clockwise, then slowly turn the adjuster back in the clockwise direction. (This adjustment is required to prevent too much power being applied to the AOM elements.) Stop at the limit position if the brightness does not fall to 98% when the adjuster is turned fully counter-clockwise for the Ar laser.

6) The AOM controller adjustment procedure is left here. Turn on the AOM controller and set the Laser Control Bar voltage to 1V. Next, proceed to "3.4 Laser Rough Adjustment 2" (P.115) using the first-order light from each laser.

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3.4 Laser Rough Adjustment 2

1 Attaching the dichroic mirror adjustment unit

1) Attach the three dichroic mirror adjustment units, aligning the pin positions and partially tightening the two screws on each unit. The following color mirrors are attached for each laser:

• L1 (405, 633): Violet

• L2 (488): Blue

• L3 (543): Total reflection

2) Attach the spring units while pressing them against the side of the dichroic mirror adjustment unit.

3) Rotate the dichroic mirror adjustment unit by hand to check that it rotates smoothly. If the lower face of the dichroic mirror adjustment unit does not contact the laser unit, the rotation will not be smooth.

Press.

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2 Adjusting the Ar dichroic mirror adjustment unit angle

(The dichroic mirror adjustment unit adjusts the laser angle.)

1) Place the dichroic mirror centering tool (for the EX) in front of the fiber coupling.

2) Loosen the two horizontal rotation clamp screws of the Ar dichroic mirror adjustment unit.

3) Adjust the horizontal axis set screws and align the horizontal axis of the laser light with the tool opening position. If the AOM is attached, first turn off the AOM controller and check the zero-order light position. Always check that the first-order (strong) light is aligned with the hole in the tool. Check that the first-order light is used for subsequent adjustments.

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4) Loosen the Ar dichroic mirror adjustment unit vertical clamp screw. The L1 and L3 clamp screws are spring plungers. (On products manufactured after May 2004) Unscrew the screws by approximately 90° from the fully-tightened position to tension the spring.

5) Adjust the vertical axis set screw and align the vertical axis of the laser light with the tool opening position.

6) Carefully tighten the horizontal clamp screws.

7) If the beam becomes misaligned from the center after tightening, fine-adjust the vertical set screw to align the beam with the tool opening.

8) Carefully tighten the vertical clamp screw.

This completes the adjustment of the Ar dichroic mirror adjustment unit.

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3 Removing the front sheet metal cover

Remove the three screws and remove the front sheet metal cover.

4 Coarse-adjusting the angle of the other two dichroic mirror adjustment units

1) As a target, prepare a card on which crosshairs are drawn. Place the target at least 50 cm from the fiber attachment unit, aim the Ar laser, and clamp it at the position at which the beam strikes the center of the target.

2) Using the procedure in “2. Adjusting the Ar dichroic mirror adjustment unit angle”, adjust the dichroic mirror adjustment units vertically and horizontally so that the beams from the other two lasers also roughly coincide with the target.

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5 Adjust the laser polarization orientations (except for 405 laser)

1) Place the polarizer-equipped tool in front of the fiber coupling with the index facing forward.

2) Open the Ar laser shutter to emit the Ar laser only.

3) Place a white paper behind the fiber coupling and move the polarizer knob to provide minimum light transmission.

4) Open the shutters for and emit the other two lasers.

5) Rotate the laser heads for these two lasers to find the position that gives the darkest results. Do not alter the polarizer orientation from the position determined for the Ar laser.

6) Tighten the slot-tightening screws (1 each) on the two other lasers after adjusting the polarizer orientation, then tighten one sleeve-clamping screw above the center.

7) Remove the polarizer-equipped tool.

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6 Fine-adjusting the angle of the other two dichroic mirror adjustment units and adjusting the beam shift unit

1) Adjust the angle of the dichroic mirror adjustment unit and align the He-Ne laser beam position with the target positioned at a distance of 50 cm. Since the cross-section of the 405 cylindrical laser beam is upright elliptical, estimate a center point and use this for alignment.

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2) Adjust the beam shift unit to align the positions of the other laser beams with the position of the Ar laser beam scattered by the mirror reflector in front of the fiber coupling. Adjust horizontally either by rotating the beam shift unit by hand or by inserting an Allen wrench in the side and turning the horizontal axis set screw. Adjust vertically by moving the vertical axis set screw up or down with the clamp screw loosened.

3) Repeat steps 1) and 2) to make the light from the three lasers coincide with the Ar laser beam position on the mirror reflecting surface and the target position at a distance of 50 cm. (Take enough time to perform this step thoroughly. Getting the laser beams to coincide here will make it easier to adjust transmission into the optical fiber.)

Horizontal axis set screw

Vertical axis set screw (farther from axis A)

Vertical axis clamp screw(closer to axis A)

Mirror reflector

Axis A

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3.5 Final Adjustment for the Ar Laser Light with the Optical Fiber

1 Centering the Ar laser

The Ar laser beam is aligned with the coupler centering tool pinhole.

1) Loosen the clamp screw on the coupler unit.

2) Set the coupler centering tool pinhole in the laser incident direction, place a card on the emission side, and adjust the H and V laser incidence side adjustment screws to find the point giving maximum brightness.

3) Set the coupler centering tool pinhole in the direction of laser emission and adjust the H and V fiber side adjustment screws to find the point giving maximum brightness.

4) Repeat steps 2) and 3) two or three times to find the point of maximum brightness.

Laser incidence side adjustment screw

Fiber side adjustment screw

Clamp screw(center)

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2 Attaching the fiber coupling

1) Close all laser shutters.

2) Tighten the three screws and attach the fiber coupling unit. Since this will be rotated at a subsequent step, do not tighten excessively at this time. Tighten so that the units can still be rotated by inserting the tool in the tool opening. Make sure that the key slot in the fiber coupling is on top. Handle the coupling carefully to keep out debris.

3 Transmitting Ar laser light through the fiber

Never look directly at the fiber tip. Always monitor laser emissions by aiming the tip down onto a sheet of paper.

1) Carefully align the end of the fiber (the end without the tag) with the key slot in the coupling and insert as far as it will go. Tighten the fiber connector. Gradually tighten, stopping when light is first emitted from the tip of the fiber.

2) If no light is emitted from the end of the fiber in step 1), loosen the fiber connector approximately 10 degrees, gradually pull out the fiber, and observe whether light is emitted. (Light is emitted more easily if defocused.) If this is not effective, repeat the steps of loosening the connector and pulling the fiber a couple of times.

WARNING

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3) If even a small amount of laser light is observed, adjust the two fiber side adjustment screws on the coupling to find the point at which the Ar laser light increases in intensity. (Final fine adjustment will be performed after adjusting the polarizing orientation.)

4) Gradually tighten the fiber connector. Stop just before the point at which the light emission is extinguished. Now adjust the two fiber side adjustment screws to find the point giving a maximum light intensity. Repeat this procedure to ensure a maximum intensity with the fiber connector tightened.

5) If no light is emitted even after repeating these steps, repeat the procedure from step 1 in “3.5 Final Adjustment for the Ar Laser Light with the Optical Fiber.”

6) Tighten the fiber connector at the point giving a maximum Ar laser light brightness. Check that there is no play in the fiber.

7) Close the Ar laser shutter.

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4 Adjusting the fiber polarizing orientation

1) Align the knob on the polarizer-equipped tool with the index. Mount the polarizer-equipped tool on the polarizing orientation adjustment tool with the knob at the top.

2) Clamp the emission end of the optical fiber to the polarizing orientation adjustment tool. Place a light-scattering target (such as paper) before it. Position the fiber so that it is not subjected to excessive force, and so that both ends are laid out straight.

3) Open the Ar laser shutter. Insert a tool into the coupling and rotate while monitoring the laser light image on the target to find the point giving the minimum brightness. (If the three screws on the coupling are too tight, loosen them slightly before rotating.)

4) Place the power meter in front of the polarizer to measure the laser power.

Coupling clamping screw

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5) Rotate the polarizer-equipped tool by 90 degrees so that the knob faces to the side, place the power meter in front of the polarizer, and measure laser power.

6) Check that the extinction ratio (ratio of bright to dark laser power) is at least 20:1. If not, repeat the adjustment procedure from step 1.

7) Tighten the three clamping screws on the coupling.

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5 Adjusting to give maximum Ar laser light transmission through the fiber

1) Attach the emitter end of the optical fiber to the power meter tool and adjust to find the point giving the maximum brightness, while measuring the brightness on the power meter. To adjust the brightness, use either the laser incidence side H adjustment screw (horizontal) or V adjustment screw (vertical), together with the fiber side V adjustment screw and H adjustment screw. As a guide to Ar laser incidence efficiency, the brightness at the end of the coupler should be at least 50% of the brightness in front of the coupler.

(Transmitting the light through the AOM unit will distort the beam shape and result in a lower transmission ratio than without the AOM unit.)

(Adjustment example)

(1) Turn the laser incidence side H adjustment screw 30 to 60 degrees in one direction.

(2) Turn the fiber side H adjustment screw in the same direction as (1) to find the point giving a maximum brightness. If brightness does not increase, turn the laser incidence side adjustment screw 30 to 60 degrees in the direction opposite to (1). Turn the fiber side adjustment screw in the same direction to find the point giving a maximum brightness.

(3) Repeat the adjustment in step (2) to find the point in the horizontal axis giving a maximum brightness.

(4) Turn the laser incidence side V adjustment screw 30 to 60 degrees. Turn the fiber side V adjustment screw in the same direction to find the point giving a maximum brightness.

(5) Repeat the adjustment in step (4) to find the point in the vertical axis giving a maximum brightness.

(6) Repeated adjustments along the horizontal and vertical axes may result in even greater brightness.

Fiber side adjustment screw

Laser incidence side adjustment screw

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2) Tighten the two clamp screws on the coupler, and then tighten the rotation clamp screw.

3) Record the Ar laser brightness. (Note the power meter wavelength setting.)

4) Open the other laser shutters and check that laser light is emitted. If no light is emitted from the other lasers, repeat the adjustments from P.107, “3-2 Adjusting the He-Ne laser and 405 cylindrical laser” in “3.3 Laser Rough Adjustment 1.”

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3.6 Final Adjustment for the He-Ne Laser Light and 405 Laser Light with the Optical Fiber

The procedure given below describes how to transmit laser light through the fiber by adjusting the dichroic mirror adjustment unit and beam shift unit alone, without using the coupler.

1) Open the shutter only for the laser to be used. Close the shutters on the other lasers.

2) Adjust the horizontal and vertical axis set screws on the dichroic mirror adjustment unit while monitoring the brightness on the power meter to find the point giving a maximum brightness. To reduce tightening deviations, tighten the two horizontal axis clamp screws to the point at which the dichroic mirror is able to rotate before adjusting horizontal rotation.

3) Adjust the beam shift unit to find the point giving a maximum brightness. Adjust horizontally either by rotating the beam shift unit by hand or by inserting an Allen key in the tool hole. Adjust vertically by moving the vertical axis set screw up or down with the clamp screw loosened. Approximately 45% should be achieved in front of and behind the fiber coupling for each laser. (Transmitting the light through the AOM unit will distort the beam shape and result in a lower transmission ratio than without the AOM unit.) For the 405 cylindrical laser, this figure is approximately 10%.

Tool hole

Vertical axis clamp screw Vertical axis set screw

Horizontal axis clamp screws

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4) Carefully tighten the beam shift unit vertical axis clamp screw. Readjust the rotation axis on the beam shift unit if the brightness decreases here. Then carefully tighten the horizontal axis clamp screw.

5) Once the point giving a maximum brightness has been found, carefully tighten the two horizontal axis clamp screws alternately on the dichroic mirror adjustment unit. If the brightness decreases after tightening, readjust the vertical axis.

6) Carefully tighten the vertical axis clamp screw. Excessive tightening is not necessary if large tightening deviations occur.

7) Record the brightness for each laser.

8) Close all the laser shutters.

When the AOM unit will not be used, proceed to "3.8 Attaching the Sheet Metal Cover." To use the AOM unit, continue to "3.7 Adjusting the AOM Controller."

Horizontal axis clamp screw

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3.7 Adjusting the AOM Controller

1 Adjusting the AOM controller bias

1) Record the brightness at the fiber tip when the Laser Control Bar reads +1V.

2) Set the Laser Control Bar to 0V.

3) Very slowly turn the AOM driver bias adjuster clockwise until brightness is reduced to half the level for 1V.

4) Reset the Laser Control Bar to +1V and measure the brightness at the fiber tip. If the brightness measured in step 1) has decreased, measure the new brightness.

5) Set the Laser Control Bar to 0V and readjust the bias adjuster until brightness is half the reduced level. Adjust carefully here to ensure that brightness is 50% ±2.5% of the brightness at 1V. Adjust slowly. Immediately after the bias adjuster is turned to a specific setting, brightness will fluctuate briefly.

2 Attaching the AOM controller cover

1) Attach the cover to the AOM controller.

2) Turn on the REMOTE switch on the rear of the AOM controller.

3 EZ-C1 setup

1) Set LASER POWER to 100%.

2) Set TYPE to <calibration table>.

3) Check [Interpolate between calibration points]. Set Brand according to the laser wavelength to be used.

4) Close.

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4 Checking brightness adjustment accuracy

1) Set the Laser Control Bar to 100% and record the brightness. (Value A)

2) Set the Laser Control Bar to 50% and record the brightness. (Value B) Check that B/A × 100 is 50% ±2.5%. If not, repeat the AOM controller bias adjustment procedure.

This completes the AOM controller adjustment.

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3.8 Attaching the Sheet Metal Cover

1) Attach four harness bands to the laser unit mount.

2) Secure the cables and fibers with harness bands.

3) Attach the sheet metal cover using the six screws.

4) Align the knob with the control range indicator for the light control knob.

Harness bands

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5) Affix the label provided corresponding to the laser wavelength used.

This completes the laser adjustment.

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Controller IX

When using the three-detector unit, DIH interlock cable (MXA22046), inverted microscope interlock unit (MHB47100), or the transmission detector unit, the following options must be installed.

Precautions during setup

• Turn off the power switches of all devices when connecting wires.

• Do not open the cover of the detector unit while PMT is applied with gain. Doing so activates the excessive-light protection circuit and forcibly reduces the PMT gain to zero.

1 Installing the Second Line-grabber Board (MHE41900)

The line-grabber board mounted in the controller is provided with two input channels. When using the three-detector unit or transmission detector unit with the system, an additional line-grabber board must be installed.

(1) Checking the second line-grabber board The board ID jumper and connector indications on the second line-grabber board differ from those on the standard line-grabber board (i.e., on the first line-grabber board). Check the indications to make sure that the correct line-grabber board has been added. JP1: OUT JP2: IN J1: Affixed with a “G” sticker J2: Without sticker

(2) Inserting the second line-grabber board (see Fig. IX-1)

1) Remove the side panel from the controller. Two (upper and lower) screws are located on the back side and another two on the bottom face.

2) Remove the bracket from slot 5.

3) Insert the second line-grabber board into slot 5.

(3) Connecting the 3-SYNC cable (see Fig. IX-1)

1) Remove the 2-SYNC cable connected between the sync-control board and the first line-grabber board.

2) Connect the 3-SYNC cable to the sync-control board, the first line-grabber board, and the second line-grabber board.

3) Reattach the side panel to the controller.

CAUTION

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Fig IX-1 Side view

SYNC-control board

I-LOCK connectors (Interior of the controller) (For the DIH, one connector is used.)

I-LOCK connector

I/O board 3-SYNC cable

First line-grabber board

Second line-grabber board

J6

J4

J5

J5

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2 Connecting the Cable for the Interlock Unit (MHB47100) for Inverted Microscopes

Installing the interlock unit requires the installation of the I-LOCK cable in the controller.

(1) Installing the cable in the controller (see Fig. IX-1)

1) Remove the side panel from the controller. Two (upper and lower) screws are located on the back side and another two on the bottom face.

2) Remove the cover from the I-LOCK connector on the rear panel inside the controller.

3) Connect the I-LOCK cables to the terminals of the I-LOCK connector.

4) Remove the jumper pin from J6 on the I/O board, and connect the I-LOCK cable.

5) Reattach the side panel to the controller.

• When the I-LOCK connector is open, the interlock is engaged to prevent

the laser-unit shutters from opening.

(2) Operation check Refer to “2. Checking the TE2000 Interlock Unit” in “XI. General Adjustment.”

3 Connecting the DIH Interlock Cable (MXA22046)

(1) Installing the cable in the controller

1) Remove the side panel from the controller. Two (upper and lower) screws are located on the back side and another two on the bottom face.

2) Remove the cover from the I-LOCK connector on the rear panel inside the controller.

3) Connect the I-LOCK cable to one terminal of the I-LOCK connector.

4) Remove the jumper pin from J6 on the I/O board, and connect the I-LOCK cable.

5) Attach the cover provided (white) to the other terminal of the I-LOCK connector.

6) Reattach the side panel to the controller.

• When the I-LOCK connector is open, the interlock is activated to prevent

the laser-unit shutters from opening.

(2) Operation check Refer to “3. Checking the DIH Interlock” in “XI. General Adjustment.”

CAUTION

CAUTION

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4 AOM Unit SYNC Cable Connection

Turn off power before connecting the cable.

Connect the SYNC cable inside the C1 controller as follows:

(1) Remove the controller side panel. There are two screws at the rear and two at the bottom.

(2) Remove the top PCI bracket on the rear of the controller.

(3) Install the SYNC connector bracket provided into the top slot.

(4) Connect the cable to the SYNC-control board (longest board) J5 connector (10-pin, brown).

(5) Attach the controller side panel.

I-LOCK connector

I/O board3-SYNC cable

SYNC-control board

First line grabber board

CI-SYNCconnector

CAUTION

Top bracket

Second line grabber board

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Connecting Cables Between Units X

Precautions when making connections

• The scan head and it’s controller are adjusted in combination. Make sure that the scan head and controller being used have the same serial number.

• Turn off the power switches of all devices when connecting connectors.

• Use a dedicated AC outlet for the argon laser, as it requires a large current.

• Do not open the cover of the detector unit while the PMT is applied with gain. Doing so activates the excessive-light-protection circuit and forcibly reduces the PMT gain to zero.

1 Connect cables between units, as shown in Fig. X-1 and Table X-1. The cables for the laser unit, scan head, and detector unit are enclosed in equipment packages.

2 Connect the 100 base T cross wired cable (to be arranged by the distributor) between the controller and PC’s LAN connector.

3 Connect the power cable (to be arranged by the distributor) to the controller.

CAUTION

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Fig. X-2 Connector panel (SHV)

C1

C3

C5

C8

C9

C6

C7

C10

C12

C15

C14

C17

C16

C18

P9

P2

P5

P6

P7

P8

D1 D2

C1 controller PC Standard detector unit

P4

P3P1

C4

C2

C13

C11

A1

S1

S2

S3

S4

S5

Laser unit AOM controller

L1 L2 A2 A3 A4

A6

A5

Transmission detector (manual operation type)

Transmission detector(motorized switch type)

TD1TD1

TD2

C1 scan head(SHV)

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Fig. X-2 Connector panel (SHS)

P9

P2

P5

P6

P7

P8

D1 D2

P4

P3P1

C3 C4

C1

C6

C7

C8

C19 C10

C11

C16

C5

C12

C15 C17 C18

C1 controller PC Detector unit

C14 C13

C9

C2

A1

Laser unit AOM controller C1 scan head(SHS)

L1 L2 A2 A3 A4

A6

A5

S1 S2

S4

S6

S3

S5

Spectral detector Transmission detector(manual operation type)

Transmission detector (motorized switch type)

SP1

SP2

TD1TD1

TD2

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Table X-1 Table of Cable Connections

Unit No. Name Connect to Remarks

C1 Power supply

C2 LAN P8

C3 I/O S5

C4 Y galvanometer S4

C5 X galvanometer S3

C6 Interlock TE2000 For DIH of 80i/90i

C7 Interlock TE2000

C8 Laser shutter L2

C9 AOM (synchronization signal) A2

C10 Detector D2 For standard detector

C11 Trigger output External device

C12 Trigger input External device

C13 R signal D1

C14 B signal D1

C15 G signal D1

C16 Not used -

C17 Transmission detector TD2 Only for transmission detector with motorized switch type

C18 Transmission detector fiber TD1

Controller

C19 Spectral detector SP2

P1 Power supply

P2 Keyboard

P3 Mouse

P4 Printer

P5 RS232C z motor etc.

P6 Monitor

P7 LAN (network)

P8 LAN (controller) C2

PC

P9 AOM (control signal) A3

D1 Image signal C13,14,15 Standard detector

D2 Control signal C10

L1 AOM A6 Laser

L2 Shutter C8

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Unit No. Name Connect to Remarks

A1 Power supply

A2 C1-SYNC (synchronization signal) C9

A3 PC (control signal) P9

A4 Not used -

A5 LD power control 408LD etc.

AOM

A6 AOM control L1

SP1 Detector fiber (FC) S6 Spectral detector

SP2 Signal out C19

TD1 Light guide C18 Transmission detector

TD2 Control C17 Only for transmission detector with motorized switch type

S1 Laser fiber

S2 Detector fiber (SMA) For standard detector

S3 X galvanometer C5

S4 Y galvanometer C4

S5 I/O C3

Scan head

S6 Detector fiber (FC) For spectral detector

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General Adjustment XI

The following adjustments and confirmations must be made with the system and operation software running.

• Confirm that there is no one in the area before turning on the lasers.

• Avoid looking at laser light emitted from the objective during scanning when you are in the vicinity at the laser.

• Since shading adjustment involves working under laser safety class 2 conditions, the lasers must be handled as described below:

• When a green He-Ne laser is installed, use only this laser to make adjustments.

• When no green He-Ne laser is installed, use the Ar laser in Standby mode with ND4 set to IN (or equivalent light-reduced state).

• When making adjustments while observing the fluorescent image, use the specified objective and make sure that the intensity of the laser light emitted has been suitably reduced before starting. Use an adequately bright specimen, like an HE-stained specimen or a fluorescent plastic specimen. The light should be reduced approximately as follows when using a bright specimen:

• Ar laser: RUN mode, 20 mW, ND8 + ND4 (or ND8 only, depending on specimen)

• Green He-Ne: ND4 (or no ND depending on specimen)

• Make sure that all of the sockets on the nosepiece are filled up with the

centering tool for pupil observation, objectives or cover caps before

starting this process.

• Do not remove neither the diascopic illumination lamphouse or the

cover cap on the right side port (TE2000-E/U) to avoid laser radiation.

These procedures involve the final system inspection and adjustment. Check once again that the system is correctly assembled and connected before starting.

1 Confirmation of the Standard Detector Unit

• Make sure that the second and third dichroic mirrors are installed in the

standard detector unit.

• Confirm the following for all channels:

WARNING

CAUTION

WARNING

CAUTION

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(1) PMT (photomultiplier tube) confirmation

Introduce room light into the tip of the optical fiber for the fluorescent light to be

attached to the scan head. Using the operation software, gradually increase the PMT

gain. Confirm that the image brightness increases.

If the image brightness does not increase, check the optical fiber connection, the

channel of the displayed image, and the detector unit cable connection.

(2) Light-shield confirmation

Attach the protective cap on the SM connector on the tip of the optical fiber for the

fluorescent light to be attached to the scan head.

Using the operation software, increase the PMT gain to 140 and confirm that the image

brightness is not increased from the value when the PMT gain is set to 0.

If the image brightness increases, confirm that the protective cap is attached securely

and that the detector unit cover is completely closed.

After confirming the above, screw the optical fiber for the fluorescent light into the scan head

and attach the sheet metal cover.

Fig. XI-1

Optical fiber for the excitation light

Optical fiber for the fluorescence light

Sheet metal cover

Optical fiber for the spectral detector

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2 Checking the TE2000 Interlock Unit

When the C1 is attached to the TE2000, attach and inspect the interlock unit as follows: Activate the interlock function by turning the dial of the shutter module to the “Bino” position or by tilting the illumination pillar (see Fig. XI-2), and make sure that all laser shutters in the laser unit close. Reset the interlock (turn the dial of the shutter module to the “Confocal” position and move the illumination pillar to the upright position) and turn OFF the Interlock Status button on the Laser and Detector toolbar of the operation software. Then confirm that the system returns to the normal condition (i.e., is ready for image acquisition). In case of abnormal conditions, check the cable connections.

Fig. XI-2

Tilting the illumination pillar makes all laser shutters in the laser unit close.

Turning the dial to the “Bino” side makes all laser shutters in the laser unit close.

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3 Checking the DIH Interlock

The interlock cable must be connected and checked as described below when the C1 is attached to the DIH. For usual C1 observations, both knobs are pushed in on the DIH as shown in the photo below. Pulling out the upper knob (indicated by the arrow in the photo) will activate the interlock. Confirm that the motorized shutter on the laser unit closes in conjunction when the upper knob is pulled out. Confirm that the system returns to a ready state for image capture when you press in the knob once again and turn the Interlock Status Button to OFF state on the Laser and Detector toolbar in the system software.

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4 Adjusting the Scan Head Mount

Adjust the inclination of the head to minimize the brightness reduction (shading) at the periphery. The specific adjustment procedure will differ slightly from microscope to microscope.

4.1 Upright Microscope

When attached to DIH (80i/90i)/dedicated trinocular eyepiece tube (80i/90i/E600)

(1) Preparation

1) As shown in Fig. XI-3, set the centering tool for pupil observation on the nosepiece. Place a mirror on the stage to enable observation of the centering tool.

2) Turn on the He-Ne laser (green, yellow, or red).

3) Using the operation software, set the scan range to 0, then stop the scan.

4) Using the operation software, open the shutter and confirm that a circular pupil is projected onto the centering tool.

Fig. XI-3

All of the sockets on the nosepiece should be filled up with objectives or cover caps.

Centering tool for pupil observation

Mirror

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(2) Adjustment

1) Referring to the concentric circles on the centering tool, note the extent and direction of the deviation of the pupil position, as shown in Fig. XI-4. (The centering tool is marked in 1-mm graduated intervals.)

2) Determine the amount of inclination by shifting the origin index mark from the reference index mark on the vertical tube by a distance corresponding to the deviation indicated on the tool. Loosen set screw B, rotate the scan head and vertical tube mount according to the above scale indication. Then tighten set screw B (see Fig. XI-9). (Example) If the deviation as viewed from the front of the microscope is as shown in Fig. XI-4, the center is shifted 1 mm toward a, as illustrated in Fig. XI-6. In this case, move the origin index mark one division on the scale from the reference index mark, as shown in Fig. XI-8, and tighten set screw B. If the deviation is 0.5 mm, move the origin index mark one half-division, and tighten the set screw.

3) As illustrated in Fig. XI-8, the midpoint between the black reference index mark and the black origin index mark represents the position of maximum inclination. As shown in Fig. XI-7, determine the inclination by rotating the entire vertical tube so that the midpoint between the two index marks moves in the direction b, 180° opposite the direction of a.

Loosen set screws A and C. Holding the scan head in place with your hand, rotate the entire vertical tube in the direction determined in the above step. Be careful not to damage the scan head cable.

4) Tighten set screws A and C to secure the vertical tube and scan head in place (see Fig. XI-9).

Pupil of the centering tool, projected by the mirror (View from front of microscope) Center is deviated one division (1 mm) in direction a.

Fig. XI-4

a

45°

Pupil image from above

Reference index mark (black)

Fig. XI-5

Midpoint between two index marks

Origin index mark (black)

Rotate the entire vertical tube.Move the midpoint between two index marks in the opposite direction to a, and fix the vertical tube.

Mount

Vertical tube

Top view of vertical tube

Fig. XI-6

a

45°

a

Fig. XI-7

b

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(3) Confirmation

1) Make sure that the deviation of the pupil position is 0.5 mm or less, as measured by the centering tool.

2) If the deviation is larger than 0.5 mm, loosen set screws A and C. While supporting the scan head with your hand, slightly turn the entire vertical tube to perform fine adjustment. If this adjustment fails to reduce the deviation to the specified level, repeat the procedure described in “(2) Adjustment.”

3) When adjustment is complete, make sure all the set screws are firmly tightened.

For deviation of one division on centering tool Move the origin index mark one division (1 mm) from the reference index mark.

Position of maximum inclination (midpoint between two index marks) Origin index mark (black)

Reference index mark (black)

Mount

Vertical tube

Set screw C Set screw B

Set screw A

Fig. XI-8 Fig. XI-9

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When attached to dedicated beam splitter (E1000/E800)

• Note that the following adjustment procedure differs from that for the

eyepiece tube for the E600 and the TE mount.

(1) Preparation

1) As shown in Fig. XI-10, set the centering tool for pupil observation on the nosepiece. Place a mirror on the stage to enable observation of the centering tool.

Fig. XI-10

2) Turn on the He-Ne laser (green, yellow, or red).

3) Using the operation software, set the scan range to 0 and stop the scan.

4) Using the operation software, open the shutter and confirm that a circular entrance pupil is projected onto the centering tool.

All of the sockets on the nosepiece should be filled up with objectives or cover caps.

Centering tool for pupil observation

Mirror

CAUTION

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(2) Adjustment

1) Referring to the concentric circles on the centering tool, note the direction of deviation of the pupil position from the front of the microscope, as shown in Fig. XI-11.

2) While observing the direction of the pupil deviation, simultaneously turn the two set screws positioned on the diagonal line and tilt the scan head so that the pupil approaches the concentric circles on the centering tool. (Example) If the pupil on the centering tool is shifted as shown in Fig. XI-11, the pupil image is shifted as indicated in Fig. X-12 when viewed from above. As shown in Fig. XI-13, while keeping set screws A1 and 2 lightly tightened, simultaneously rotate two Allen wrenches in the direction that causes set screw B1 to tighten and in the direction that causes set screw B2 to be pulled out.

Pupil image from above

(observed from the front of the microscope)

Fig. XI-12

Fig. XI-13

Pupil of the centering tool, reflected by the mirror (View from the front side of the microscope) Center is deviated one division (1 mm) in direction a.

Fig. XI-11

a

45°

a

45°

After adjustment, cover the adjustment holes of set screws A1, A2, B1, and B2 with the urethane caps provided.

Set screw B1 (tilt)

Direction a

Set screw C (for securing the C1 head)

Set screw A1 (tilt)

Set screw A2 (tilt)

Set screw C (for securing the C1 head)

Set screw B2 (tilt)

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3) To move the objective’s pupil 90 degrees from direction a, rotate set screws A1 and A2 in opposite directions to tilt the scan head to bring the pupil closer to the center of the tool. A1 and A2 are located on a diagonal line different from the diagonal on which the previously rotated set screws (B1 and B2) were located.

4) Repeat steps 2) and 3) until the pupil is positioned within 0.5 mm of the concentric circles on the tool. When adjusting set screws, be sure to simultaneously rotate the two set screws located on a given diagonal line.

• Do not confuse the set screw C for securing the C1 head with location of

the hole for tilting adjustment.

(3) Confirmation

1) Make sure that the deviation of the pupil position is 0.5 mm or less, as measured by the centering tool.

2) If the deviation is larger than 0.5 mm, repeat steps 2) and 3) in “2 Adjustment” above.

3) After adjustment, tighten the set screws in four locations.

4) Cover the four set screw holes with black caps (provided with the beam splitter). Be careful not to block the scan-head mounting holes.

5) Loosen set screws A and C. Supporting the scan head with your hand, turn the vertical tube slightly to perform fine adjustment. If this adjustment fails to reduce the deviation to the specified level, repeat the procedure described in “(2) Adjustment.”

6) When adjustment is complete, make sure that all set screws B and C (two upper locations) and set screws A (two locations) are firmly tightened.

Fig. XI-14

CAUTION

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4.2 Inverted Microscope

When attached to the side port (TE2000E/U)

(1) Preparation

1) As shown in Fig. XI-15, set the centering tool for pupil observation on the nosepiece of the TE2000, and project the pupil onto the centering tool. In the case of an inverted microscope, a mirror is not used, since the pupil position can be observed directly.

Fig. XI-15

(2) Adjustment

1) Referring to the concentric circles on the centering tool, note the extent and direction of deviation of the pupil position, as shown in Fig. XI-16.

2) Determine the amount of inclination by shifting the origin index mark from the reference index mark on the straight tube by a distance corresponding to the deviation indicated on the tool. Loosen set screws B and C, then, while manually supporting the scan head so that it is pressed against the microscope side, rotate the straight tube mount according to the above scale indication. Tighten set screw B (see Fig. XI-21). (Example) If the center is shifted 1 mm in the direction of a, as shown in Fig. XI-16, the deviation as viewed from the side is as shown in Fig. XI-18. In this case, as shown in Fig. XI-19, move the origin index mark one division on the scale from the reference index mark and tighten the set screw B. If the deviation is 0.5 mm, move the origin index mark a half-division and tighten the set screw.

Centering tool for pupil observation

All of the sockets on the nosepiece should be filled up with objectives or cover caps.

Pupil of the centering tool (View from front of the microscope) Center is deviated one division (1 mm) in direction a.

Fig. XI-16

a

45°

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3) The midpoint between the black reference index mark and the black origin index mark is

the position of maximum inclination. As shown in Fig. XI-19, determine the direction of

inclination by rotating the entire straight tube so that the midpoint between the two

index in the direction b, 180° opposite the direction of a when viewed from the side port.

Loosen set screws A and C. Holding the scan head in place with your hand, rotate

the entire straight tube in the direction determined in the above step, then tighten

set screws A and C (at the two upper locations). When tightening set screws A and C,

keep the scan head pressed against the side port (see Fig. XI-21).

Be careful not to damage the scan head cable.

(3) Confirmation

1) Make sure that the deviation of the pupil position is 0.5 mm or less, as measured

by the centering tool.

2) If the deviation is larger than 0.5 mm, loosen set screws A and C. While

supporting the scan head with your hand, turn the straight tube slightly to

perform fine adjustment.

If this adjustment fails to reduce the deviation to the specified level, repeat the

procedure described in “(2) Adjustment.”

3) When adjustment is complete, make sure all the set screws are firmly tightened.

Fig. XI-17

Mount

Straight tube

Top stage surface image viewed from the side

Fig. XI-18

a

45°

Fig. XI-19

Origin index mark (black)

Reference index mark (black)

Move the midpoint between two index marks in the opposite direction to a, and fix the straight tube.

b Midpoint between two index marks

Rotate the entire straight tube.

Viewed from the side port

1

Set screw B

Fig. XI-21

Set screw C

Set screw A Reference

index mark (black)

Origin index mark (black)

Mount Straight tube

Fig. XI-20

Position of maximum inclination (midpoint between two index marks)

For deviation of one division on centering tool scale, move the origin index mark one division (1 mm) from the reference index mark.

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5 Adjustment and Confirmation of the Fluorescent Image

Adjustment and confirmation method

Place the second dichroic mirror in the detector unit. Perform a fluorescent confocal observation of a uniform fluorescent specimen and perform the following adjustment and confirmation procedure.

(1) Adjustment of the collector lens in front of the pinhole

1) Move the collector lens adjustment screw gradually until the center of the image is at its brightest.

2) To obtain the value of the brightness at the center of the image, use the analysis tool provided in the operation software.

Image acquisition conditions for adjustment

• Scanning mode: 512 x 512, 1x zoom

• Field of view: Maximum

• Objective: 60x or higher

• Pinhole: Small

• Method of obtaining brightness value: Using the analysis tool, position a circular spot with an area of about 32,000 (i.e., a radius of approx. 100 pixels) at the center of the image and display the average brightness.

(2) Confirmation of shading adjustment status After adjusting the collector lens in front of the pinhole as described above, check the following: The image acquisition conditions in this confirmation process are the same as those described above.

• There should be no noticeable shading (i.e., irregularities in brightness).

• Changing the focus should result in concentric circular blurring, not blurring in eccentric ways. (Note: check the tilting of the specimen.)

If either condition is not satisfied, repeat the procedures described in “4. Adjustment of the Scan Head Mount.”

Caution for checking the image and the accuracy with the spectral detector unit

To check the image and the accuracy with the spectral detector unit, be sure to comply with instructions below. If not, the accuracy will be inferior to the specifications.

Measurement conditions

• Warm-up time: approx. 40 minutes

• Temperature and humidity of the environment: 18°C to 28°C, 60% or less (with no

condensation)

• Measurement wavelength: 420 to 750 nm [applicable wavelength: 400 to 750 nm]

• PMT gain: 142 (-500 V) to 255 (-900 V)

[programmable range: 114 (-400 V) to 255 (-900 V)]

• Pixel dwell: 4 to 30 µs [programmable range: 4 to 60 µs]

Fig. XI-22

Collector lens adjustment screws (2 locations)

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6 Compensating the Image Offset for the Bi-directional Scanning

Adjust the image offset for the bi-directional scanning by altering the sampling timing.

(1) Preparation

1) Preparing a specimen

To make offsets easier to assess, prepare specimens with prominent vertical lines. A grid micro specimen (reflection observation) is ideal.

2) Setting the scanning direction

• Changing the scanning direction setting in the middle of adjustment will

offset the adjustment values. Make any changes in the scanning direction before adjusting.

For mounting on an inverted microscope (TE2000), you must invert the scanning

direction of the X and Y scanners. To invert the scanning direction of the X and Y scanners, select Configure Confocal C1 Mirrors Mirror Option from the EZC1 menu and check the options for "X direction" and "Y direction" for "Mirror image."

Do not check these options when using an upright microscope.

Fig. XI-23

CAUTION

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(2) Adjustment

1) Acquiring an image

Acquire the image for the specimen provided using the following conditions.

• Scanning mode: 512 × 512, bi-directional scanning

• Field of view: Maximum field of view (zoom ×1)

Fig. XI-24

• When adjusting with a reflected image using a grid micro specimen,

remove all detector unit filters and detect using channel 2.

Image size

Bi-directional scanning

Field of View

CAUTION

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2) Correcting image offset (delay time setting)

• Allow the system to warm up for at least 5 minutes after switching on

before performing the following procedures.

If the bi-directional images are offset, as shown in Fig. XI-26, display the

Bi-Directional Scan toolbar shown in Fig. XI-25 using Tools Bi-Directional Scan on the EZC1 menu, and alter the value of "Adjust Scan" to minimize the image offset. This value will be stored automatically in the PC as an INI file.

Fig. XI-25

Fig. XI-26

CAUTION

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3) Altering the field of view to correct the image offset

Set the field of view to 95% and output the bi-directional image. Click next to the handle section of the field of view slider to adjust in 5% intervals. Adjust the image offset as shown in section 2). Repeat this correction for 90%, 85%, 80%, and so on. (5% pitch)

Fig. XI-27

4) Resizing the image to correct the image offset Set the image size to 256 and correct in the same way as for 2) and 3).

Note:

The data shown in the table below will be stored in the PC by this adjustment.

Correction data stored in INI file

Field of view Image size

0%~ 5%~ 10%~ 15%~ ・・・・ 95%~ 100%

512x512 ・・・・

256x256 ・・・・

Click here to change the value in 5% decrements.

Field of view 95%

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7 Microscope Settings for the Diascopic DIC Microscopy

To observe the DIC image with the transmission detector unit, the microscope must be equipped with the DIC Set. See your microscope’s setup manual for information on DIC setup. Note also that polarized light is emitted from the optical fiber as usual. Therefore, the microscope’s analyzer is not used. When using your transmission detector unit in conjunction with the TE2000, turn off the illuminator’s diffuser to provide sufficient brightness. When using your transmission detector unit in conjunction with the Eclipse 80i/90i or E800/E1000, E600, turn on the diffuser (since sufficient brightness is already secured). Remove the NCB filter from the optical path. Though the ND filters should be removed from the optical path, they can be used for the brightness adjustment if the image is too bright even after the gain is reduced by the adjustment of the laser brightness or the switching of the objective. The optical fiber for the excitation-light (silver) should be fixed on the desk or some other place to prevent it from receiving any excessive force.

• The DIC images derived from this system should present more uneven

brightness compared to the DIC images of the usual DIC microscopes, especially when the low powered objective is used.

• If the light amount exceeds the limit because the gain is too high, the image slowly changes to white and black. If this happens, decrease the gain. It will take several seconds till the image recovers.

Accessories required for the microscope

<Eclipse 80i/90i, E800/E1000, or E600>

Condenser: 80i/E800/E1000/E600: D-CUD universal condenser unit (dry) 90i: D-CUD-E motorized universal condenser unit (dry) Polarizer: Rotating polarizer for the V-PP DIC Nosepiece: C-ND DIC nosepiece DIC prism: DIC prism set Objective: DIC objective

<TE2000>

Condenser: LWD condenser or high N.A. condenser Polarizer: T-P polarizer Nosepiece: T-ND6 DIC 6 nosepiece or T-ND6-E motorized DIC6 nosepiece DIC prism: Differential interference contrast condenser module, DIC slider Objective: DIC objective

Adjusting the microscope

You need to adjust the focus and centering of your condenser according to your microscope’s manual. Fully open the aperture diaphragm, but open the field diaphragm only in accordance with the target field of view. (Stopping down the field diaphragm excessively will cause vignetting.) Adjust the installation position of the polarizer using the analyzer and the halogen light source so that they will be set in the Crossed Nichols position when observed through the eyepieces.

CAUTION

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8 Accuracy Check of the Spectral Detector Unit

The accuracy of the spectral detector unit must be checked at the time of installation. For detailed information about the measurement method and it’s criteria, refer to the technical information provided separately.

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Confirmation and Check Sheet EntryXII

The person setting up the system should check the details listed in the sheet below and enter the results. The completed sheet should be sent to the supervisor.

Serial number:

1. Setup and user

Setup date (Month) (Day) (Year)

Setup location

Person performing setup

User Name:

Address:

Contact:

Date manufactured (Month) (Day) (Year)

2. Product configuration

No. Item Description

1 Manufacturer

2

PC

Model

3 EZ-C1 Version number

4 Ring adapter S L

5

Head

First dichroic mirror

C1-LU2S C1-LU3S C1-LU3EX 6 Unit

C-LU2 C-LU3 C-LU3EX

7

Laser

Laser type L1:

L2:

L3:

8 Unit DUD(2) DUT(3) Spectral

9

Detector

Filter 1:

2:

10 Main unit

11

Microscope

Fluorescent device Yes/No

12 Transmission Yes (Manual, Motorize)/No

13 AOM Yes/No

14

Option

Z Focus Yes/No

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3. Safety checks

No. Unit Check items Check

1 Is there a gap between the unit and laser head? Y N

2 Is the protective cover securely attached? Y N

3

Laser

Does any laser light leak from gaps in the protective cover? Y N

4 Head Is the sheet metal cover attached? Y N

5 Are all the head-retaining screws securely tightened? Y N

6 Are all the retaining screws on the accessories (eyepiece tube, fluorescent device, nosepiece, condenser, diascopic illumination, and so on) securely tightened?

Y N

7 Are objectives mounted in all the openings in the revolving nosepiece? Y N

8 Does the laser shutter close when the interlock activates? Y N

9

Microscope

Are labels affixed at the specified locations? Y N

10 Standard Detector

Are the flange-retaining screws securely tightened? Y N

11 Is the transmission detector adapter securely attached to the microscope?

Y N

12 Is the diascopic lamphouse securely attached to the transmission detector adapter?

Y N

13 Is the transmission detector fiber securely attached to the adapter? Y N

14

Option

Is the transmission detector fiber securely attached to the detector? Y N

4. Power check

No. Check items Value

1 Fiber efficiency: Intensity comparison for each laser immediately before and after transmission into a single-mode fiber.

%

2 Aperture intensity: Intensity immediately beneath the objective lens for each laser at maximum power output.

mW

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Performance and SpecificationsXIII

System configuration • Microscope: Eight types are available. • Laser safety tube: Use the tube appropriate for the microscope. • Z focus unit (optional): Use the unit appropriate for the microscope. • Laser unit: six types are available. • AOM unit (optional): Only for Three-laser unit EX • Laser: 14 types are available. • Scan head: Use the scan head with for the controller and spectral head

included. • Ring adapter: Use the adapter appropriate for the microscope. • First dichroic mirror: Five types are available. (A special BS is also

available for the SHS.) • Controller • Standard detector unit: Tow types are available. • Spectral detector unit • Fl-filter: Five types are available. • Transmission detector option; Depends on the microscope to be used. • PC unit: AT compatible machine, monitor (SXGA), keyboard, mouse • Network cable: 100 Base-TX • Software: EZ-C1 ver.3.0 or higher

Microscope unit Microscope

• Upright microscope

Eclipse 80i/90i: Use the DIH eyepiece tube with the interlock cable or the C1-dedicated trinocular eyepiece tube.

E1000/E800: Use the C1-dedicated beam-splitter. E600/E600FN: Use the C1-dedicated trinocular eyepiece tube.

• Inverted microscope TE2000E: Use the C1-dedicated shutter and the C1- dedicated

illumination pillar switch. TE2000U: Use the C1-dedicated shutter and the C1- dedicated

illumination pillar switch.

Motorized focus Eclipse 90i, TE2000E: Linear encoder 50-nm step E1000: Rotary encoder 50-nm step Z drive: Stepping motor 50-nm step

Light source Laser unit

• Two-laser unit S, two-laser unit

Number of lasers: 2 units Light control: ND4 and ND8 provided for each laser Shutter: For each laser beam

• Three-laser unit S, three-laser unit Number of lasers: 3 units Light control: ND4 and ND8 provided for each laser Shutter: For each laser beam

• Three-laser unit EX Number of lasers: 3 units Light control: 11 levels of step adjustment/continuous adjustment, AOM (optional) Shutter: For each laser beam Function: Ar wavelength switch

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Laser option • Violet (405-408) Manufacturer: Coherent Head: RADIUS 405 Power supply: CDRH control box, 15W max. Manufacturer: Melles Griot Head: 56 ICS 423 Power supply: 56 IMA 016, 7.5W max.

• Violet (440-445) Manufacturer: Melles Griot Head: 56 ICS 426 Power supply: 56 IMA 016, 7.5W max.

• Blue (488-514) Manufacturer: Spectra-physics Head: 163-C02 Power supply: 263-C04, 20A max. Manufacturer: Spectra-physics Head: 163-C12 Power supply: 263-C04, 20A max. Manufacturer: Spectra-physics Head: 161C-030 Power supply: 263-C04, 10A max. Manufacturer: Melles Griot Head: IMA 101040 Power supply: Model 175B Manufacturer: Coherent Head: Sapphire 488-20 Power supply: CDRH controller, 60W max.

• Green (543) Manufacturer: Melles Griot Head: 25 LGP 193 Power supply: 05LPL951-065/100V, 05LPL915-065/115-230V

• Yellow (561) Manufacturer: Melles Griot Head: 85YCA010 Power supply: 85YCA series control box

• Yellow (594) Manufacturer: JDS Uniphase Head: 1677P Power supply: 1207-1/115V, 1207-2/230V, 1207-3/100V

• Red (633-640) Manufacturer: JDS Uniphase Head: 1125P Power supply: 1202-3/100V, 1202-1/115V, 1202-2/230V Manufacturer: Melles Griot Head: 56 ICS 272 Power supply: 56 IMA 015 Manufacturer: Melles Griot Head: 56 ICS 264 Power supply: 56 IMA 015

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Scan • Two control-type galvano-scanner mirrors

With standard detector unit • Speed: 2 frame/second (512 x 512) • Pixel: 16 x 16 to 2048 x 2048 • Scan Zoom: Zooming up to 1/126 of full field of view • Scan area: Up to 12.7 mm x 12.7 mm on the first image • Scan mode: L-T, X-Y, X-Y-T, X-Y-Z, X-Y-Z-T, spot, mirror image,

reciprocation, and rotation

With spectral detector unit (only for the SHS) • Speed: 1 frame/second (512 x 256) • Pixel: 16 x 16 to 512 x 512 • Scan Zoom: Zooming up to 1/126 of full field of view • Scan area: Up to 12.7 mm x 12.7 mm on the first image • Scan mode: L-T, X-Y, X-Y-T, X-Y-Z, X-Y-Z-T, spot, mirror image, and

rotation

Detector Scan head

Pinhole: Four motorized switches: S, M, L, O First dichroic mirror: Six types

Detector unit Spectral detector unit (only for the SHS)

Detector: 32ch, PMT x 1 Wavelength resolution pitch: 3 steps (2.5, 5, 10 nm) Wavelength range: 420 to 750 nm (applicable wavelength range: 400 to 750 nm) Excitation light mask: Automatic type

Standard detector unit

• Two-detector unit Detector: Two PMTs Two filter cube positions

• Three-detector unit Detector: Three PMTs Three filter cube positions

Transmission detector unit

Detector: PMT Five different fl-filter sets

A/D Dynamic range: 12 bits

Controller Input ratings: 100-240VAC, 50/60Hz, 3A Fuse ratings: 250VAC, 5A (time-lag) Protection class: Class 1 Pollution degree: Degree 2 Installation: Category II

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PC • Main unit OS: Microsoft Windows 2000 with Service pack 4 or higher or XP Professional with Service pack 2 or higher CPU: Intel Pentium 4 processor (3.2 GHz or higher processor recommended.)Memory: 1 GB min. (recommended) Network board: Intel 82550-compatible Graphics card: 24 bit, 1280 x 1024 pixels min. Other: CD-ROM drive

• Accessories Monitor: SXGA or higher, 24-bit color

Weight • Laser unit Two-laser unit S, two-laser unit: 13 kg Three-laser unit S, three-laser unit: 27 kg Three-laser unit EX: 24 kg

• Scan head: 2 kg • Standard detector: 3 kg • Spectral detector: 23kg • Controller: 15 kg

Environmental conditions

SHV Operating conditions: (At the normal operation) +5°C to +35°C (with no condensation)

Storage conditions: -20°C to +60°C (with no condensation)

SHS Operating conditions: (At the normal operation) +5°C to +35°C (with no condensation) (At the measurement) +18°C to +28°C (with no condensation)

Storage conditions: -20°C to +35°C (with no condensation)

Transport conditions: -20°C to +50°C (with no condensation)

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XIII Performance and Specifications

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Product safety • Laser safety IEC60825-1 Class 3B FDA Class IIIB

• CE marking Low Voltage Directive: EN61010-1, EN60825-1 EMC Directive: EN61326 (EN55011 Group 1 Class B, EN61000-4-2/4-3/4-4/4-5/4-6/4-8/4-11), EN61000-3-2/3-3

• Australia EMI: AS/NZS2064 Class B • Complies with FCC: Part 15B Class A

This equipment has been tested and found to comply with the limits for a Class A digital devices pursuant to Part 15 of the FCC Rules.

These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment. This equipment generates, uses, and can radiate radio frequency energy and if not installed and used in accordance with this instruction manual, may cause harmful interference to radio communications. Operation of this equipment in a residential area is likely to cause harmful interference in which the user will be required to correct the interference at the user’s own expense.

This Class A digital apparatus meets all requirements of the Canadian Interference-Causing Equipment Regulations.

Cet appareil numérique de la Classe A respecte toutes les exigences du Réglement sur le matériel brouilleur du Canada.

Export regulations The spectral detector unit and the AOM optionally incorporated into the laser unit are classified as a controlled product (including provisions applicable to controlled technology) under foreign exchange and foreign trade control laws of Japan. You must obtain government permission and complete all required procedures before exporting this system.