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Non Homologous End Joining

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Page 1: Non Homologous End Joining. Homologous Recombination Non Homologous End Joining

Non Homologous End Joining

Page 2: Non Homologous End Joining. Homologous Recombination Non Homologous End Joining
Page 3: Non Homologous End Joining. Homologous Recombination Non Homologous End Joining

Homologous Recombination

Non Homologous End Joining

Page 4: Non Homologous End Joining. Homologous Recombination Non Homologous End Joining
Page 5: Non Homologous End Joining. Homologous Recombination Non Homologous End Joining
Page 6: Non Homologous End Joining. Homologous Recombination Non Homologous End Joining

Differences between HR and NHEJ:

• Error- free / Error- prone• Cell cycle regulated • Varies between species

HR NHEJ

yeast mammalsplants

Page 7: Non Homologous End Joining. Homologous Recombination Non Homologous End Joining

HR NHEJ

MHC rearrangementMeiosis

Cell-induced Double Strand Brakes

Page 8: Non Homologous End Joining. Homologous Recombination Non Homologous End Joining

V(D)J recombination

Page 9: Non Homologous End Joining. Homologous Recombination Non Homologous End Joining

NHEJ Defects

• SCID (Severe Combined Immune Deficiency).

• Cancer – genome instability: rearrangements, deletions, amplifications.

Page 10: Non Homologous End Joining. Homologous Recombination Non Homologous End Joining

Ku 70/80 heterodimer

Page 11: Non Homologous End Joining. Homologous Recombination Non Homologous End Joining

Ku has a role in telomeres

?

Page 12: Non Homologous End Joining. Homologous Recombination Non Homologous End Joining

KU 70/80

Page 13: Non Homologous End Joining. Homologous Recombination Non Homologous End Joining

Ku is critical for telomere maintenance

Page 14: Non Homologous End Joining. Homologous Recombination Non Homologous End Joining

Ku Plays Several Roles at Telomeres

Telomere addition

Telomeric silencing

Page 15: Non Homologous End Joining. Homologous Recombination Non Homologous End Joining

Ku is a multifunctional protein

Page 16: Non Homologous End Joining. Homologous Recombination Non Homologous End Joining

HR

NHEJ


Homologous Series Members

In Vivo Blunt-End Cloning Through CRISPR/Cas9-Facilitated ...1 In Vivo Blunt-End Cloning Through CRISPR/Cas9-Facilitated Non-Homologous End-Joining Jonathan M. Geisinger, Sören Turan,

ARCHIVES OF...recombination repair (HR), and non-homologous end-joining repair (NHEJ)11. XRCC4 gene is found on the chromosomal 5q14.2 and it is involved in precise end-joining of

Organization and dynamics of the nonhomologous end-joining

Is Homologous Recombination

CRISPR Genome Editing: Considerations for Therapeutic ......CRISPR Flexibility Addresses Diverse Mutations Non-homologous end joining typically disrupts a gene or eliminates a disease-causing

Relative rates of homologous and nonhomologous ... · The deleted genomes presumably arose from nonhomologous end joining after breakage of input molecules, as observed previously

Summary of Homologous Series

Homologous Series, Formulae & IUPAC

The repair of a double-strand break in DNA by homologous end- joining. Damaged site is copied from the other chromosome by recombination proteins

DEMONSTRATION OF HOMOLOGOUS RECOMBINATION EVENTS … · DEMONSTRATION OF HOMOLOGOUS RECOMBINATION EVENTS IN THE EVOLUTION OF BOVINE VIRAL DIARRHOEA ... good chance for homologous

Mechanism of Eukaryotic Homologous Recombination

DNA Damage and Mutation in Population Studies Workshop ... · DNA repair pathways (nucleotide excision repair, base excision repair, non-homologous end-joining, and mismatch repair)

Change of Gene Structure and Function by Non-Homologous End- Joining, Homologous Recombination, and Transposition of DNA. Presenta: ELIZABETH HERNÁNDEZ

Upset Protrusion Joining Techniques For Joining Dissimilar ...€¦ · Overview •Start date: September 30, 2013 •End date: December 31, 2016 •Percent complete: ~40% •F- “High-volume,

Olaparib and temozolomide in SCLC · 2018. 5. 8. · PARP inhibition in SCLC. • PARP1 regulates base excision repair, homologous recombination, and non-homologous end joining. Inhibition

Generation of an Alpha-1 Antitrypsin Knockout Mouse Model ... · Homologous End Joining (NHEJ). With 5 copies of the SerpinA gene in the mouse genome, the CRISPR/Cas9 system offered

Research Paper ATM independent, high fidelity ... · Research Paper 1 under homologous end joining (NHEJ) [6]. Ataxia telangiectasia mutated (ATM), ATM and Rad3-related (ATR), and

Staff Training/Joining time.pdf · take joining time at the end of the vacation. (b) If vacation is combined with leave, joining time shall be regulated under Rule 9.1 (b) (i), if

Insights into the non-homologous end joining pathway and

End-to-end mechanical joining of tubular components · End-to-end mechanical joining of tubular components Guilherme Batalha Reis da Gama Nunes ... There are many different welding

Heterologous Cas9 and non-homologous end joining as a Potentially Organism … · Cas9 and NHEJ into a Potentially Organism-Agnostic Knockout (POAK) system, I first combinedCas9,aCRISPRarray(forgRNAproduction),andNHEJontoasingleplasmid

The non-homologous end-joining activity is required for ...as DNA replication, cell-cycle control, and DNA damage response and repair [9–11]. Recent studies suggested that the FA

DNA break repair by homologous recombination Homologous Recombination · 2007-01-01 · Homologous Recombination target replaced with donor DNA sequence ! induced break ! donor DNA

CAT’s views on classification of ATMPs: homologous use · Organ transplantation BMT Pancreatic iPSc islest Bone marrow in bone healing CD34 in myocardium . 6 Homologous vs/Non homologous

Homologous Recombination (HR)

Research Paper DNA-PKcs Negatively Regulates Cyclin B1 ... · non-homologous end -joining pathway of DNA double -stranded break repair. DNA -PKcs has also DNA -PKcs has also been

Emerging IC Packaging Technologies - SMTA. Materials HighHigh-End FC End FC. ... Target devices Logics on Si interposer ... Die Joining Adaptive Learning

Direct Involvement of Retinoblastoma Family Proteins in DNA Repair by Non-homologous End-Joining

Replicação na presença de erros. Recombination at the Molecular Level Breakage and joining also directed by enzymes. Homologous recombination

Development of NTLA-1001: First-in-Class, LNP-CRISPR/Cas9 … · 2020-04-29 · Editing Strategy Relies on Knockout Caused By Error-Prone Non-Homologous End Joining (NHEJ) of a Double-Strand

CHARACTERIZATION OF APLF IN NON-HOMOLOGOUS END-JOINING · 4.4 The FHA and PBZ domains are not required for the nuclear localization of APLF 80 4.5 Mapping the APLF-binding site on

facilitate homologous recombination and contributes to DSB … · promotes end resection to facilitate homologous recombination and contributes to DSB repair within heterochromatin

Homologous genes

Two-Stage Synapsis of DNA Ends during Non-homologous End ... · repair by non-homologous end joining (NHEJ) in Xenopus laevis egg extract. Synapsis of DNA ends is found to proceed