Novocastra HD - HematopathologyHighly Definitive Antibodies

Trust the HD Difference – Highly Definitive Antibodies for Diagnostic Confidence• Independently benchmarked against leading equivalents by external QA*

• Establishing a new quality standard in IHC; simplifying antibody selection

*Independent analysis commissioned by Leica Biosystems and conducted by NordiQC according to the manufacturers’ instructions for use and on the corresponding staining platform.

Anaplastic Large Cell LymphomaStrong nuclear and cytoplasmic staining in the neoplastic cells.

Human Follicular LymphomaNeoplastic cells show a moderate, predominantly cytoplasmic staining reaction.

Human Diffuse Large B Cell LymphomaThe majority of neoplastic cells show a moderate to strong nuclear staining reaction.

Human ThymomaNeoplastic cells show a moderate to strong and distinct membrane staining reaction.

Human Lymph Node, T Cell LymphomaNeoplastic cells show a moderate and distinct predominantly membrane staining reaction. Occasional plasma cells show a strong staining reaction.

T Cell LymphomaMembrane staining of tumor cells.

T Cell LymphomaTumor cells show a strong membrane staining reaction.

T Cell LymphomaIntense membrane staining of tumor cells.

BOND 7 mL PA0306

Liquid 0.5 mL NCL-L-ALK

Anaplastic Lymphoma Kinase (ALK)

Clone 5A4

BOND 7 mL PA0117

Liquid 1 mL NCL-L-bcl-2

Bcl-2 Oncoprotein Clone Bcl-2/100/D5

BOND 7 mL PA0204

Liquid 1 mL NCL-L-Bcl-6-564

Bcl-6 Oncoprotein Clone LN22

BOND 7 mL PA0235

Liquid 0.5 mL NCL-L-CD1a-235

Liquid 1 mL NCL-L-CD1a-235

CD1a Clone MTB1

CD3 Clone LN10

BOND 7 mL PA0553

BOND 30 mL PA0122

Liquid 1 mL NCL-L-CD3-565

CD4 Clone 4B12

BOND 7 mL PA0427

Liquid 0.5 mL NCL-L-CD4-368

Liquid 1 mL NCL-L-CD4-368

BOND 7 mL PA0168

Liquid 0.5 mL NCL-L-CD5-4C7

Liquid 1 mL NCL-L-CD5-4C7

CD5 Clone 4C7 CD7 Clone

LP15

BOND 7 mL PA0266

Liquid 1 mL NCL-L-CD7-580

Novocastra HD - Hematopathology

BOND 7 mL PA0843

Liquid 0.5 mL NCL-L-CD19-163

Liquid 1 mL NCL-L-CD19-163

Human Lymph Node, T Cell LymphomaNeoplastic cells show a moderate to strong, distinct membrane staining reaction.

Human Lymphoblastic LymphomaIntense membrane staining of neoplastic lymphoid cells.

Human Hairy Cell LeukemiaMembrane staining of malignant cells.

Hodgkin’s Disease, Mixed CellularityMixed cellular membrane staining and characteristic staining of paranuclear hofs of Reed Sternberg cells.

Pre B Acute Lymphoblastic LymphomaNeoplastic cells show a moderate to strong predominantly membrane staining reaction.

Mantle Cell LymphomaIntense membrane staining of malignant lymphocytes.

Follicular B Cell LymphomaFollicular B cell lymphoma showing a strong membrane staining reaction.

Human TonsilNK cells and CD4/CD8 double positive T cells show a weak to moderate and distinct membrane staining reaction while the majority of lymphocytes are unstained.

Mantle Cell LymphomaNeoplastic cells show a moderate and distinct predominantly membrane staining reaction. Occasional plasma cells show a strong staining reaction.

Hodgkin’s LymphomaNeoplastic cells show a moderate and distinct predominantly membrane staining reaction. Occasional plasma cells show a strong staining reaction.

Human LymphomaMembrane staining of endothelial cells.

Acute Myeloid LeukemiaNeoplastic cells show a moderate and distinct predominantly membrane staining reaction. Occasional plasma cells, as expected, show a strong staining reaction.

CD19 Clone BT51E

CD8 Clone 4B11

BOND 7 mL PA0183

Liquid 0.5 mL NCL-L-CD8-4B11

BOND 7 mL PA0270

BOND 30 mL PA0131

Liquid 1 mL NCL-L-CD10-270

CD10 Clone 56C6

BOND 7 mL PA0554

Liquid 1 mL NCL-L-CD11c-563

BOND 7 mL PA0473 NEW

CD11c Clone 5D11 CD15 Clone

MMA

CD20 Clone L26

BOND 7 mL PA0200

BOND 30 mL PA0359

Liquid 0.5 mL NCL-L-CD20-L26

Liquid 1 mL NCL-L-CD20-L26

BOND 7 mL PA0169

Liquid 0.5 mL NCL-L-CD23-1B12

Liquid 1 mL NCL-L-CD23-1B12

CD23 Clone 1B12

BOND 7 mL PA0790

Liquid 1 mL NCL-L-CD30-591

CD30 Clone JCM182

CD31 Clone JC70A

BOND 7 mL PA0414

Liquid 1 mL NCL-L-CD31-607

BOND 7 mL PA0555

Liquid 1 mL NCL-L-CD33

CD33 Clone PWS44

BOND 7 mL PA0042

Liquid 0.5 mL NCL-L-LCA

Liquid 1 mL NCL-L-LCA

CD45 Clone X16/99

BOND 7 mL PA0191

Liquid 1 mL NCL-L-CD56-504

CD56 Clone CD564

Human AppendixIntense staining of plasma cells and secreted immunoglobulin A.

Myeloid SarcomaSelective medium to strong staining of neoplastic cells.

Mantle Cell LymphomaMantle cell tumor cells show moderate staining, with a stronger reaction seen in heavy chain expressing plasma cells.

Control Tissue, Human ColonIntense staining of the epithelium in the crypts.

Human Mantle Cell LymphomaNuclear staining of malignant pre-germinal B lymphocytes.

Human TonsilGerminal centre macrophages show a strong cytoplasmic staining reaction, while the interfollicular macrophages show correct weak to moderate staining reaction.

PlasmacytomaIntense membrane staining of tumor cells.

Human Gastrointestinal Stromal TumorIntense membrane staining of tumor cells.

Human Diffuse Large B Cell LymphomaMembrane staining of tumor cells.

CD68 Clone 514H12

BOND 7 mL PA0273

Liquid 1 mL NCL-L-CD68

BOND 7 mL PA0007

Liquid 1 mL NCL-L-CD117-032

BOND 7 mL PA0599 NEW

CD117 (c-kit Oncoprotein)

Clone EP10 CD79a Clone

JCB117

BOND 7 mL PA0088

CD138 Clone MI15

Liquid 1 mL NCL-L-CD163 NEW

CD163 Clone 10D6 Cytokeratin 20 Clone

Ks20.8 Cyclin D1 Clone EP12

BOND 7 mL PA0022

BOND 30 mL PA0037

Liquid 0.5 mL NCL-L-CK20

Liquid 1 mL NCL-L-CK20

BOND 7 mL PA0046 NEW

Immunoglobulin A Clone N1CLA

Liquid 1 mL NCL-L-IgA

Immunoglobulin D Clone DRN1C

BOND 7 mL PA0061 He Only

Liquid 1 mL NCL-L-IgD

Invasive Breast CarcinomaIntense staining of invasive tumor cells.

Cytokeratin 7 Clone RN7

BOND 7 mL PA0942

BOND 30 mL PA0138

Liquid 1 mL NCL-L-CK7-560

Human Skin, Kaposi’s SarcomaNeoplastic cells show moderate cytoplasmic staining, while normal endothelial cells show strong staining.

Factor VIII related antigen (von Willebrand Factor)

Clone 36B11

BOND 7 mL PA0055 NEW

Liquid 1 mL NCL-L-vWF

Immunoglobulin G Clone RWP49

BOND 7 mL PA0905 NEW

Liquid 1 mL NCL-L-IgG

Human TonsilCytoplasmic staining of the plasma myeloma cells.

LeicaBiosystems.com

Novocastra HDHighly Definitive Antibodies

Benefits of BOND Ready-to-Use antibodies

✓ Optimized for staining performance on BOND

✓ Validated antibody concentrations

✓ Ease of use: just plug and play

✓ Increase standardization through automation

✓ Eliminate variability in antibody preparation

Benefits of concentrated liquid antibodies

✓ Flexibility of use on various platforms1

✓ Adaptable to a variety of detection systems1

✓ Adjustable concentration to suit user’s tissue1

✓ Economical option that retains confidence in clone selection

1. The performance of antibodies should be validated when used with automated platforms or manual staining systems other than stated on Instructions for Use.

Improve workflow efficiency and streamline validation.

95.11988 Rev F · 03/2016 · Copyright©2016 Leica Biosystems Newcastle Ltd. All rights reserved. LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH. Novocastra and BOND are trademarks of Leica Biosystems and its affiliates. Other logos, product and/or company names might be trademarks of their respective owners. The EP clones have been created by Epitomics Inc., using Epitomics’ proprietary rabbit monoclonal antibody technology covered under Patent No.’s 5, 675, 063 and 7, 402, 409.

Pre B Cell Acute Lymphoblastic LeukemiaTumor cells show a strong, nuclear staining reaction.

Pre B Cell Acute Lymphoblastic LeukemiaNeoplastic cells show a strong and distinct nuclear staining reaction.

Human Kidney, Wilms’ TumorNuclear staining of invasive tumor cells.

BOND 7 mL PA0552

Liquid 1 mL NCL-L-PAX-5

Pax-5 Clone 1EW

BOND 7 mL PA0339

Liquid 0.5 mL NCL-L-TdT-339

Liquid 1 mL NCL-L-TdT-339

Terminal Deoxynucleotidyl Transferase (TdT)

Clone SEN28

BOND 7 mL PA0562

Liquid 1 mL NCL-L-WT1-562

Wilms’ Tumor Clone WT49

BOND 7 mL PA0129

Liquid 1 mL NCL-L-MUM1

Multiple Myeloma Oncogene 1 (MUM-1)

Clone EAU32

Hodgkin’s LymphomaA moderate to strong predominantly nuclear staining reaction is seen in the Reed-Sternberg cells and plasma cells.

B Cell Chronic Lymphocytic LeukemiaNeoplastic cells show a moderate and distinct predominantly membrane staining reaction. Occasional plasma cells show a strong staining reaction.

High Grade B Cell Immunoblastic LymphomaIntense nuclear staining in proliferating B cells.

Kappa Light Chain Clone CH15 Ki67 Clone

MM1

BOND 7 mL PA0606 He Only

Liquid 1 mL NCL-L-KAP-581

BOND 7 mL PA0118

BOND 30 mL PA0410

Liquid 1 mL NCL-L-Ki67-MM1

Lambda Light Chain Clone SHL53

BOND 7 mL PA0570 He Only

Liquid 1 mL NCL-L-LAM-578

B Cell Chronic Lymphocytic LeukemiaNeoplastic cells show a moderate and distinct predominantly membrane staining reaction. Occasional plasma cells show a strong staining reaction.

Immunoglobulin M Clone 8HC

BOND 7 mL PA0278 NEW

Liquid 1 mL NCL-L-IgM NEW

Normal Human LarynxIntense membrane staining of lymphocytes.