nsf-itr meeting 10-05 miniaturized sensors air molecules aerosols water molecules
DESCRIPTION
NSF-ITR Meeting 10-05 Miniaturized Sensors Air molecules aerosols Water molecules cells, viruses. Sensor Operation Sensor Specific Initial event Transduction to a signal Measurement of signal Communication of result. Initial Event Binding of analyte - PowerPoint PPT PresentationTRANSCRIPT
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NSF-ITR Meeting 10-05
Miniaturized Sensors
Airmoleculesaerosols
Watermoleculescells, viruses
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Sensor Operation
Sensor Specific Initial event
Transduction to a signal
Measurement of signal
Communication of result
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Initial Event
Binding of analyte
Interrogation of free analyte
Chemical reaction
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Binding of Analyte
GasChemical or physical adsorption
Metal oxides & semiconductors
BiologicalsAntibodiesNucleic acidsLigands
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Interrogation of free analyte
GasIonizationSpectroscopy
Light, microwave, massWater
SpectroscopyLight, microwave, mass
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Transduction to a SignalElectrical
ConductanceImpedance spectroscopyvoltametry
Light & MicrowavesAbsorptionFluorescence
Resonance OscillatorsMechanical motionSurface acoustic waves
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Gas Ionization
Modi, et al., Nature, 424, 171-174, 2003
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Gas Ionization
Modi, et al., Nature, 424, 171-174, 2003
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Complex Mixtures
Difficult to distinguish components
Signal of one component can swampothers
Difficult to standardize
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A Possible Solution
Fractionate the analyte stream
Differentiate molecules based on size, shape or charge
On-chip family of membranes
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On-chip Family of Membranes
Controlled filtration characteristics
Pattern membrane characteristics
Biocompatible
Use with or without electrophoresis
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Two Membrane Types Under Evaluation
Cellulose AcetateSpin cast
AluminaNanofabricated
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Filtration Characteristics vs Preparation
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Purification: DNA from HemeMembrane + electrophoresis
Mohamed, et al., Chromatography A, 2005
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Proposed Selective Gas SensorOn-chip membrane & ionization
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An Antibody Based Cell Sensor
Binding agent - immobilized antibody
Electrical measurement - impedance
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Number of captured CD4+ cells vs Impedance
Mishra, et al., Biosensors & Bioelcectronics, 2005.
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Amperometric biosensor using ion channel proteins
4 LAYERS
4. Protein: sensing component
3. Lipid membrane: provides the proper environment for the sensor proteins
2. Hydrogel: structural (localization and stability)
1. Electrode: Detection
4
321
I analyte