nurhayati icomp surabaya
TRANSCRIPT
Fakultas Peternakan Universitas Jambi
Kampus Pinang Masak KM 15 Mendalo Darat Jambi 36361 Indonesia
Extracted Pearl Grass (Hedyotis corymbosa L Lamk) as
a Feed Additive for Broiler Chicken
Dr. Ir. Nurhayati, M.Sc.agr. * 1
Dr. Madyawati Latief, S.P., M.Si **
*Faculty of Animal Science, University of Jambi
** Faculty of Agricultural Science, University of Jambi
Kampus Pinang Masak KM 15 Mendalo Darat Jambi 36361
1E-mail: [email protected], [email protected]
Nurh
ayati a
nd
M L
atie
f : Pearl g
rass a
s fe
ed
add
itive in
Bro
iler
1
Fakultas Peternakan Universitas Jambi
Kampus Pinang Masak KM 15 Mendalo Darat Jambi 36361 Indonesia
Introduction
• Pearl grass (Hedyotis corymbosa L Lamk
or Oldenlandia corymbosa)
Genus Hedyotis,
Family Rubiaceae
- white flower
- narrow leaf
- branched stems
- circle fruit
Nurh
ayati a
nd
M L
atie
f : Pearl g
rass a
s fe
ed
add
itive in
Bro
iler
2
Fakultas Peternakan Universitas Jambi
Kampus Pinang Masak KM 15 Mendalo Darat Jambi 36361 Indonesia
Pearl grass (Hedyotis corymbosa L Lamk) Nurh
ayati a
nd
M L
atie
f : Pearl g
rass a
s fe
ed
add
itive in
Bro
iler
3
Fakultas Peternakan Universitas Jambi
Kampus Pinang Masak KM 15 Mendalo Darat Jambi 36361 Indonesia
Pearl grass (Hedyotis corymbosa L Lamk)
• People use for their health to treat digestion
disorder, cancer, appendix sore, microbial
infection (bacteria, protozoa and fungi)
• Contains hentriacontan, stigmasterol, ursolic
acid, oleonic acid, ß-sitosterol, sitisterol, D-
glukosida, p-cumaric acid, flavonoid, tannin and
kumarin (Djauhariya and Hernani, 2004), 5
compounds of iridoit included Asperulosid
(Sudarsono, 1999), certain compound for
prevent tumor development (Hsu, 1998).
Nurh
ayati a
nd
M L
atie
f : Pearl g
rass a
s fe
ed
add
itive in
Bro
iler
4
Fakultas Peternakan Universitas Jambi
Kampus Pinang Masak KM 15 Mendalo Darat Jambi 36361 Indonesia
Pearl grass (Hedyotis corymbosa L Lamk)
• If it can be used for human health, could it
is used for animal to improve their
resistance, increase their production and
produce safe product for human?
• Is there any negative effect if it is used as
a feed additive for animal?
Nurh
ayati a
nd
M L
atie
f : Pearl g
rass a
s fe
ed
add
itive in
Bro
iler
5
Fakultas Peternakan Universitas Jambi
Kampus Pinang Masak KM 15 Mendalo Darat Jambi 36361 Indonesia
MATERIALS AND METHODS
• Experiment has been started since 2006
• Laboratory experiment to determine the
active compounds and antimicrobial
activities
• Feeding trial to determine the effect on
broiler chicken where their litter was
sprayed by E. coli
Nurh
ayati a
nd
M L
atie
f : Pearl g
rass a
s fe
ed
add
itive in
Bro
iler
6
Fakultas Peternakan Universitas Jambi
Kampus Pinang Masak KM 15 Mendalo Darat Jambi 36361 Indonesia
Laboratory experiment
• Activities :
- extraction, isolation, fractionation and
purification, characteristic of the structure and
antimicrobial (bacteria and fungi) activity test.
• Methods
- Chromatographic method
- Spectroscopic Method : IR, 1H and 13C NMR.
- Disk Diffusion Method : Antimicrobial activity test
Nurh
ayati a
nd
M L
atie
f : Pearl g
rass a
s fe
ed
add
itive in
Bro
iler
7
Fakultas Peternakan Universitas Jambi
Kampus Pinang Masak KM 15 Mendalo Darat Jambi 36361 Indonesia
Feeding Trial
• 100 Chickens fed
R0 : Basal diet + 0 g pearl grass extract, R1 :
Basal diet + 0.8 g pearl grass extract per kg diet,
R2 : Basal diet + 1.6 g pearl grass extract per kg
diet,
R3 : Basal diet + 2.4 g pearl grass extract per kg
diet and
R4 : Basal diet + 3.2 g pearl grass extract per kg
diet.
Nurh
ayati a
nd
M L
atie
f : Pearl g
rass a
s fe
ed
add
itive in
Bro
iler
8
Fakultas Peternakan Universitas Jambi
Kampus Pinang Masak KM 15 Mendalo Darat Jambi 36361 Indonesia
Feeding Trial
• Basal diet : starter diet (until 3 weeks of
age) and finisher diet (after 3 weeks of
age)
• After 3 weeks of age, litter was sprayed
with Escherichia coli as much as 106
CFU/100 ml of liquid agar. Then, chickens
were kept until 6 weeks of age.
• Parameters measured were feed
consumption and live weight.
Nurh
ayati a
nd
M L
atie
f : Pearl g
rass a
s fe
ed
add
itive in
Bro
iler
9
Fakultas Peternakan Universitas Jambi
Kampus Pinang Masak KM 15 Mendalo Darat Jambi 36361 Indonesia
Basal Diet Ingredients Starter Diet
(0 – 3 weeks)
Finisher Diet
(3 – 6 weeks)
Yellow corn 40.3 45.3
Polish 10.0 15.0
Soybean meal 30.0 25.0
Fish meal 15.0 10.0
Dicalsium
phosphat
1.2 1.2
Vegetable oil 2.0 2.0
Vitamin mineral
mix (Premix)
1.0 1.0
Methionin 0.5 0.5
Total 100.0 100.0
Nurh
ayati a
nd
M L
atie
f : Pearl g
rass a
s fe
ed
add
itive in
Bro
iler
10
Fakultas Peternakan Universitas Jambi
Kampus Pinang Masak KM 15 Mendalo Darat Jambi 36361 Indonesia
Basal Diet
Nutrient composition % %
Dry matter (%) 85.64 86.06
Crude protein (%) 24.12 23.26
Crude fibre (%) 3.76 3.70
Ether extract (%) 7.15 5.71
Ash (%) 6.04 5.22
Nitogen Free Extract (%) 44.57 48.16
Gross Energy (kcal/kg) 4281.74 4269.89
Metabolizable energy (kcal/kg)1 3104.26 3095.67
Nurh
ayati a
nd
M L
atie
f : Pearl g
rass a
s fe
ed
add
itive in
Bro
iler
11
Fakultas Peternakan Universitas Jambi
Kampus Pinang Masak KM 15 Mendalo Darat Jambi 36361 Indonesia
RESULTS
• Active Compounds
- 4-metil-2-en-heksanol (group of alcohol),
tersier butyl isopropyl keton (group of
keton), and stigmasta-5,22-dien-3-ol
(group of steroid).
- group of alcohol was significant, group of
keton was highly significant and group of
steroid was not significant antibacterial
activity against Escherichia coli
Nurh
ayati a
nd
M L
atie
f : Pearl g
rass a
s fe
ed
add
itive in
Bro
iler
12
Fakultas Peternakan Universitas Jambi
Kampus Pinang Masak KM 15 Mendalo Darat Jambi 36361 Indonesia
RESULTS
Parameters Treatment
Mean ± SD R0 R1 R2 R3 R4
FC (g/h/wk) 579.69 568.98 555.53 548.31 529.54 556.41 ± 19.29
LW (g/h) 1.217.50 1.231.50 1.243.88 1.265.38 1.276.88 1.247.03 ± 24.24
Carcass (%) 65.50 66.40 68.31 67.60 68.61 67.28 ± 1.31
Nurh
ayati a
nd
M L
atie
f : Pearl g
rass a
s fe
ed
add
itive in
Bro
iler
13
Fakultas Peternakan Universitas Jambi
Kampus Pinang Masak KM 15 Mendalo Darat Jambi 36361 Indonesia
CONCLUSION
Extracted Pearl grass could be used as a natural
feed additive in broiler chicken.
Nurh
ayati a
nd
M L
atie
f : Pearl g
rass a
s fe
ed
add
itive in
Bro
iler
14
ACKNOWLEDGEMENT DP2M Directorate General Higher Education for grant of
Fundamental research financial, Head of Research Centre
University of Jambi and Dean of Faculty of Animal Science
University of Jambi for permission and support, the
assistance of staffs and students of Dept. Animal Nutrition
and Feed Science, Faculty of Animal Science, University of
Jambi for the care and management of birds is acknowledged.
Fakultas Peternakan Universitas Jambi
Kampus Pinang Masak KM 15 Mendalo Darat Jambi 36361 Indonesia
Terima kasih, Thank you,
Vielen Dank, Merci, Gracias
Nurh
ayati a
nd
M L
atie
f : Pearl g
rass a
s fe
ed
add
itive in
Bro
iler
15