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Fakultas Peternakan Universitas Jambi Kampus Pinang Masak KM 15 Mendalo Darat Jambi 36361 Indonesia Extracted Pearl Grass (Hedyotis corymbosa L Lamk) as a Feed Additive for Broiler Chicken Dr. Ir. Nurhayati, M.Sc.agr. * 1 Dr. Madyawati Latief, S.P., M.Si ** *Faculty of Animal Science, University of Jambi ** Faculty of Agricultural Science, University of Jambi Kampus Pinang Masak KM 15 Mendalo Darat Jambi 36361 1 E-mail: [email protected] , [email protected] Nurhayati and M Latief : Pearl grass as feed additive in Broiler 1

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Page 1: Nurhayati icomp surabaya

Fakultas Peternakan Universitas Jambi

Kampus Pinang Masak KM 15 Mendalo Darat Jambi 36361 Indonesia

Extracted Pearl Grass (Hedyotis corymbosa L Lamk) as

a Feed Additive for Broiler Chicken

Dr. Ir. Nurhayati, M.Sc.agr. * 1

Dr. Madyawati Latief, S.P., M.Si **

*Faculty of Animal Science, University of Jambi

** Faculty of Agricultural Science, University of Jambi

Kampus Pinang Masak KM 15 Mendalo Darat Jambi 36361

1E-mail: [email protected], [email protected]

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Fakultas Peternakan Universitas Jambi

Kampus Pinang Masak KM 15 Mendalo Darat Jambi 36361 Indonesia

Introduction

• Pearl grass (Hedyotis corymbosa L Lamk

or Oldenlandia corymbosa)

Genus Hedyotis,

Family Rubiaceae

- white flower

- narrow leaf

- branched stems

- circle fruit

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Fakultas Peternakan Universitas Jambi

Kampus Pinang Masak KM 15 Mendalo Darat Jambi 36361 Indonesia

Pearl grass (Hedyotis corymbosa L Lamk) Nurh

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Fakultas Peternakan Universitas Jambi

Kampus Pinang Masak KM 15 Mendalo Darat Jambi 36361 Indonesia

Pearl grass (Hedyotis corymbosa L Lamk)

• People use for their health to treat digestion

disorder, cancer, appendix sore, microbial

infection (bacteria, protozoa and fungi)

• Contains hentriacontan, stigmasterol, ursolic

acid, oleonic acid, ß-sitosterol, sitisterol, D-

glukosida, p-cumaric acid, flavonoid, tannin and

kumarin (Djauhariya and Hernani, 2004), 5

compounds of iridoit included Asperulosid

(Sudarsono, 1999), certain compound for

prevent tumor development (Hsu, 1998).

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Fakultas Peternakan Universitas Jambi

Kampus Pinang Masak KM 15 Mendalo Darat Jambi 36361 Indonesia

Pearl grass (Hedyotis corymbosa L Lamk)

• If it can be used for human health, could it

is used for animal to improve their

resistance, increase their production and

produce safe product for human?

• Is there any negative effect if it is used as

a feed additive for animal?

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Fakultas Peternakan Universitas Jambi

Kampus Pinang Masak KM 15 Mendalo Darat Jambi 36361 Indonesia

MATERIALS AND METHODS

• Experiment has been started since 2006

• Laboratory experiment to determine the

active compounds and antimicrobial

activities

• Feeding trial to determine the effect on

broiler chicken where their litter was

sprayed by E. coli

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Fakultas Peternakan Universitas Jambi

Kampus Pinang Masak KM 15 Mendalo Darat Jambi 36361 Indonesia

Laboratory experiment

• Activities :

- extraction, isolation, fractionation and

purification, characteristic of the structure and

antimicrobial (bacteria and fungi) activity test.

• Methods

- Chromatographic method

- Spectroscopic Method : IR, 1H and 13C NMR.

- Disk Diffusion Method : Antimicrobial activity test

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Fakultas Peternakan Universitas Jambi

Kampus Pinang Masak KM 15 Mendalo Darat Jambi 36361 Indonesia

Feeding Trial

• 100 Chickens fed

R0 : Basal diet + 0 g pearl grass extract, R1 :

Basal diet + 0.8 g pearl grass extract per kg diet,

R2 : Basal diet + 1.6 g pearl grass extract per kg

diet,

R3 : Basal diet + 2.4 g pearl grass extract per kg

diet and

R4 : Basal diet + 3.2 g pearl grass extract per kg

diet.

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Fakultas Peternakan Universitas Jambi

Kampus Pinang Masak KM 15 Mendalo Darat Jambi 36361 Indonesia

Feeding Trial

• Basal diet : starter diet (until 3 weeks of

age) and finisher diet (after 3 weeks of

age)

• After 3 weeks of age, litter was sprayed

with Escherichia coli as much as 106

CFU/100 ml of liquid agar. Then, chickens

were kept until 6 weeks of age.

• Parameters measured were feed

consumption and live weight.

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Fakultas Peternakan Universitas Jambi

Kampus Pinang Masak KM 15 Mendalo Darat Jambi 36361 Indonesia

Basal Diet Ingredients Starter Diet

(0 – 3 weeks)

Finisher Diet

(3 – 6 weeks)

Yellow corn 40.3 45.3

Polish 10.0 15.0

Soybean meal 30.0 25.0

Fish meal 15.0 10.0

Dicalsium

phosphat

1.2 1.2

Vegetable oil 2.0 2.0

Vitamin mineral

mix (Premix)

1.0 1.0

Methionin 0.5 0.5

Total 100.0 100.0

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Fakultas Peternakan Universitas Jambi

Kampus Pinang Masak KM 15 Mendalo Darat Jambi 36361 Indonesia

Basal Diet

Nutrient composition % %

Dry matter (%) 85.64 86.06

Crude protein (%) 24.12 23.26

Crude fibre (%) 3.76 3.70

Ether extract (%) 7.15 5.71

Ash (%) 6.04 5.22

Nitogen Free Extract (%) 44.57 48.16

Gross Energy (kcal/kg) 4281.74 4269.89

Metabolizable energy (kcal/kg)1 3104.26 3095.67

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Fakultas Peternakan Universitas Jambi

Kampus Pinang Masak KM 15 Mendalo Darat Jambi 36361 Indonesia

RESULTS

• Active Compounds

- 4-metil-2-en-heksanol (group of alcohol),

tersier butyl isopropyl keton (group of

keton), and stigmasta-5,22-dien-3-ol

(group of steroid).

- group of alcohol was significant, group of

keton was highly significant and group of

steroid was not significant antibacterial

activity against Escherichia coli

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Fakultas Peternakan Universitas Jambi

Kampus Pinang Masak KM 15 Mendalo Darat Jambi 36361 Indonesia

RESULTS

Parameters Treatment

Mean ± SD R0 R1 R2 R3 R4

FC (g/h/wk) 579.69 568.98 555.53 548.31 529.54 556.41 ± 19.29

LW (g/h) 1.217.50 1.231.50 1.243.88 1.265.38 1.276.88 1.247.03 ± 24.24

Carcass (%) 65.50 66.40 68.31 67.60 68.61 67.28 ± 1.31

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Fakultas Peternakan Universitas Jambi

Kampus Pinang Masak KM 15 Mendalo Darat Jambi 36361 Indonesia

CONCLUSION

Extracted Pearl grass could be used as a natural

feed additive in broiler chicken.

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ACKNOWLEDGEMENT DP2M Directorate General Higher Education for grant of

Fundamental research financial, Head of Research Centre

University of Jambi and Dean of Faculty of Animal Science

University of Jambi for permission and support, the

assistance of staffs and students of Dept. Animal Nutrition

and Feed Science, Faculty of Animal Science, University of

Jambi for the care and management of birds is acknowledged.

Page 15: Nurhayati icomp surabaya

Fakultas Peternakan Universitas Jambi

Kampus Pinang Masak KM 15 Mendalo Darat Jambi 36361 Indonesia

Terima kasih, Thank you,

Vielen Dank, Merci, Gracias

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