Please refer disclaimer Overleaf.

O-Toluidine Reagent (For chlorine estimation)

R022

O-Toluidine Reagent is used for estimation of chlorine from water.

Composition**IngredientsO-Toluidine 1.0gm

Hydrochloric acid 100.0mlDistilled water 899.0ml**Formula adjusted, standardized to suit performance parameters

Directions1) Add 1ml of O-Toluidine reagent to100ml of the sample and mix thoroughly.

2) Allow the sample to stay in dark for 5-15minutes for the colour to develop if the sample is free of chloramine and nitrite

and iron are each less than 0.3ppm. If chloramine is present, allow it to stand for full 15 minutes.

3) If the sample is highly alkaline, it may give blue-green colour instead of yellow. Add 1ml of 1:4 hydrochloric acid to

reduce the pH to 4 or less.

4) Compare the colour produced with that of standards from of standard colour comparison chart.

5)Match the correct colour and report as ppm of chloride.

Principle And InterpretationA solution of O-Toluidine in hydrochloric acid react with active chlorine present in water to form a yellow product. This test

is applicable to water that have been treated with chlorine or hypochlorite such as potable water, swimming pools or

condenser water where the residual (free) chlorine range between 0-2ppm.At a pH less than 4, chlorine develops a colour with

O-Toluidine reagent, varying from pale yellow at low concentration to dark yellow at higher concentration.

Type of specimen Water samples Specimen Collection and Handling: For water samples, follow appropriate techniques for sample collection, processing as per guidelines and local standards. After use, contaminated materials must be sterilized by autoclaving before discarding.

Warning and Precautions :In Vitro diagnostic Use only. Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per established guidleines should be followed while handling clincal specimens. Saftey guidelines may be referred in individual safety data sheets

Performance and EvaluationPerformace of the product is expected when used as per the direction on the label within the expiry period when stored at recommended temperature.

Quality ControlAppearanceColourless clear solution.

ClarityClear without any precipitate.

Chlorine estimationResidual (Free)Chlorine (as ppm) is obtained by comparing the colour produced with that of the standard colour comparison chart.Colour chart for Free chlorine content (by O-Toluidine method 0-2ppm)

Intended Use

Disclaimer :

User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained inthis and other related HiMedia™ publications. The information contained in this publication is based on our research and developmentwork and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes tospecifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use butfor laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should notbe considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.

HiMedia Laboratories Technical Data

Reference1. Kuffmann F.and Moeller U.,1995,Acta Pathol .Microbiol. Scand;36:173

Revision : 02 / 2019

Storage and Shelf LifeStore between 10- 30°C in tightly closed container and away from bright light. Use before expiry date on label. On opening, product should be properly stored in dry ventilated area protected from extremes of temperature and sources of ignition. Seal the container tightly after use.

DisposalUser must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (2, 3).

2. Jorgensen,J.H., Pfaller , M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015) Manualof Clinical Microbiology, 11th Edition. Vol. 1.

3. Downes F. P. and Ito K. (Ed.), 2001, Compendium of Methods for the Microbiological Examination of Foods, 4th ed.,APHA, Washington, D.C.

In vitro diagnostic medical

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Do not use if package is damaged

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Storage temperature10°C

30°C

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